Annals of clinical and laboratory science最新文献

{"title":"Annals of Clinical and Laboratory Science: Information for Authors.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"145-146"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucoepidermoid Carcinoma Presented as a Lymph Node Metastasis in 9-Year-Old Child: A Case Report.","authors":"Ahmed Ahmed, Sibel Ak, Sara Amin, Nourhan Ibrahim, Mahmoud Elsayad","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Salivary gland neoplasms are rare in pediatric and adolescent populations. Mucoepidermoid carcinoma (MEC) is the most common malignant neoplasm of the salivary glands that most frequently arises in the parotid gland as a painless swelling. Herein we present a rare case of MEC in a child with metastasis to the cervical lymph node as primary presentation.</p><p><strong>Case report: </strong>A nine-year-old female without significant past medical history presented with a neck mass behind the ear for two months. Imaging study showed a heterogeneous soft tissue mass with a radiologic impression of a suppurative lymph node with surrounding lymphadenopathy. Histological sections of the lymph node showed metastatic carcinoma formed of sheets of epidermoid-looking cells and scattered mucin producing cells (mucocytes). Immunohistochemistry (IHC) study showed that the tumor cells were diffusely positive for Pan-Cytokeratin, p40, and p63 with focal Mucicarmine expression. Follow up positron emission-computed tomography (PET-CT) scan showed a right parotid gland mass-like lesion.</p><p><strong>Conclusion: </strong>MECs are presented in either major or minor salivary glands. The primary presentation of MEC in children and in lymph node is extremely rare. Although the IHC and molecular profiling of the MECs in the pediatric population shows similarities to those tumors observed in the adult population, the overall prognosis in the pediatric group is excellent with approximately 95% five-year overall survival rate.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"128-132"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Barriers to Blood Donation: Donor Deferrals in a Saudi Hospital Setting.","authors":"Fahd A Kuriri, Abdulrahman Ahmed, Fahad Alhumud, Fehaid Alanazi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Blood donation plays a critical role in healthcare systems by supplying blood and blood products for surgeries, emergency care, and chronic disease management. In Saudi Arabia, the prevalence of genetic disorders, like sickle cell disease and thalassemia, further increase the need for a stable blood supply. However, high rates of donor deferrals remain a significant barrier to maintaining a reliable blood supply. This study aims to analyse the main factors contributing to blood donor deferrals at King Fahad Hospital Al Hofuf between January 2023 and August 2024. By identifying and correlating key deferral reasons with donor demographics, the study seeks to provide actionable insights to reduce deferral rates and enhance donor retention.</p><p><strong>Methods: </strong>This retrospective observational study analysed blood donation records of 21,149 donors, including both eligible and deferred individuals. Demographic data (age, gender, nationality) and medical assessments (hemoglobin levels, blood pressure) were collected and analysed to identify deferral trends. Descriptive and inferential statistics were applied to assess the relationships between donor characteristics and deferral reasons, with chi-square tests and logistic regression used to identify predictors of deferral.</p><p><strong>Results: </strong>Data analyses showed that 89.5% of deferred donors were male, with the largest group of deferrals aged 21-30 years. Low hemoglobin was the most common deferral reason, affecting 27.5% of all deferred donors, with female donors disproportionately affected by anemia-related deferrals. Male donors were more frequently deferred for aspirin use (13.2%) and blood pressure issues (15.9%). Seasonal trends showed deferral peaks in January and November, while the highest donation rates were recorded in April and October.</p><p><strong>Conclusion: </strong>Addressing the higher rates of deferral due to low hemoglobin and cardiovascular issues through targeted public health interventions could significantly improve donor retention and ensure a more reliable blood supply. Implementing iron supplementation programs for female donors and cardiovascular health screenings for male donors could reduce deferral rates.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"828-836"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Müllerian Hormone Enhances Migration and Invasion in Human Trophoblast Cells.","authors":"Xuhua Hu, Depeng Zhao, Wanxue Wang, Shuhua Wu, Litong Zhu, Xuemei Li, Zhuomin Huang, Jilong Yao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Anti-Müllerian hormone (AMH) belongs to the transforming growth factor-β superfamily. Recent evidence shows that AMH and its type II receptor (AMHRII) are expressed by the placenta at term. The physiological role of AMH in trophoblast invasion and migration remains to be elucidated.</p><p><strong>Methods: </strong>Immunofluorescence and western blot were used to detect the expression of AMH and AMHRII in trophoblast cell lines and chorionic-decidual mixture from early pregnancies. <i>In vitro</i> experiments, including siRNA transfection, transwell and wound healing assays were further performed to study the function of AMH in trophoblast cell line.</p><p><strong>Results: </strong>AMH and AMHR2 are mainly expressed in the interstitial trophoblast (ITB) in human chorionic-decidual mixture of early pregnancy. We found that knocking down AMH resulted in decreased migration ability of trophoblast cells compared to the control group.</p><p><strong>Conclusion: </strong>It is shown that AMH enhances trophoblast's invasion and migration abilities. AMH may play a role in embryo implantation and placenta formation.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"790-798"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histoplasmosis in a Solid Organ Transplant Patient in a Non-Endemic Region in New York City.","authors":"Sarah Shidid, Aashna Pandya, Kateryna Karpoff, Tamar A Smith-Norowitz","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To present the case of a solid organ transplant recipient with Histoplasmosis in New York City.</p><p><strong>Case report: </strong>We present a 39-year-old female liver transplant recipient, who experienced a two-week history of progressive shortness of breath and dyspnea on exertion that interfered with all activities of daily living. Physical examination by the team revealed the patient had a WBC of 11.7, + SARS-CoV-2 NP swab, CT Chest with pneumonia, and a urine antigen test positive for Histoplasmosis. Her overall clinical picture was concerning for new Histoplasmosis infection.</p><p><strong>Conclusion: </strong>Findings endorse the diagnosis of Histoplasmosis. Histoplasmosis is rare in non-endemic regions such as New York City. An infectious workup should include fungal markers to further evaluate shortness of breath and a need to look beyond epidemiologic measures.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"873-876"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Evaluation of Urinary HE4, CEA, ProGRP, CYFRA 21-1 and NSE in the Diagnosis of Lung Cancer.","authors":"Li Zhang, Jing Zhuang, Yuxia Pang, Yinan Song, Xiaofei Li, Kuo Liu, Shuomin Li, Tao Sun","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Urinary proteins are effective tumor biomarkers. Human epididymis protein 4 (HE4), progastrin-releasing peptide (ProGRP), carcinoembryonic antigen (CEA), cytokeratin-19 fragment 21-1(CYFRA 21-1), and neuron-specific enolase (NSE) in serum, were proposed as tumor biomarkers of lung cancer. Our aim was to identify the urine protein biomarkers that can distinguish patients with lung cancer from healthy individuals and/or patients with benign lung disease with a high level of sensitivity and specificity.</p><p><strong>Methods: </strong>These urinary protein concentrations were determined from 212 patients with lung cancer, 80 patients with benign pulmonary conditions, and 100 healthy individuals.</p><p><strong>Results: </strong>In the lung cancer group, urine HE4 median concentration was approximately ten times that in the healthy and/or pulmonary benign disease group; urine CEA, CYFRA21-1, and NSE median concentrations were about eight times, twice, and half as those in the healthy group, respectively. However, the median concentrations of urine ProGRP were almost the same in the healthy, lung benign, and cancer groups. Urine HE4 showed better specificity (94.0% vs 86.0%) and sensibility (72.38% vs 60.65%) than CEA in discriminating patients with lung cancer from healthy controls. A significantly higher ROC area was also obtained with urine HE4 than with CEA (0.87 vs 0.76).</p><p><strong>Conclusions: </strong>Urine HE4 showed the best diagnostic performance, followed by CEA. The levels of urine HE4 and CEA increased significantly in patients with lung cancer, which had little relationship with pathological type and metastasis.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"845-855"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Patient with a Delayed Diagnosis of Zinc Powder Inhalation Poisoning: A Case Report.","authors":"Min Chai, Xiuxian Zang, Xinran Yang, Chenglin Yu, Bin Nian, Yixin Chen, Dilimulat Maimaiti, Shuai Chen, Dahai Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Overexposure to zinc can lead to zinc poisoning. Zinc poisoning from zinc powder inhalation has rarely been reported.</p><p><strong>Case report: </strong>A 66-year-old male presented with nausea, chest tightness, and shortness of breath. He had no symptom improvement after antibiotic treatment. The patient's history revealed recent occupational exposure to cold zinc spray. Bronchoscopy showed a high zinc level (0.5 μmol/L) in the alveolar fluid of the patient. He was diagnosed with zinc powder inhalation poisoning (ZPIP).</p><p><strong>Results: </strong>The patient received supplemental oxygen, high-dose glucocorticoids, hemoperfusion, bronchoalveolar lavage, and supportive care. Eventually, both his symptoms and a repeat lung computed tomography scan showed improvements.</p><p><strong>Conclusion: </strong>ZPIP requires a careful history recording to avoid a missed diagnosis and delayed treatments. We present this case to share our experience regarding the clinical diagnosis of ZPIP and its treatment strategies.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"886-890"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of TRIM58 Gene Methylation-Based Minimal Residual Disease Assays in Non-Small Cell Lung Cancer for Prognosis Prediction and Treatment Decision.","authors":"Zishan Wang, Wentao Hu, Jianguang Shi, Chenwei Li, Jing Guo","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To identify key genes associated with the prognosis of non-small cell lung cancer (NSCLC) through bioinformatics analysis and experimental validation, exploring the expression of the TRIM58 gene and its potential effect as a tumor suppressor.</p><p><strong>Methods: </strong>In this study, differentially expressed genes (DEGs) and differentially methylated genes (DMGs) related to lung adenocarcinoma and lung squamous cell carcinoma were selected from the TCGA dataset, with the Limma package in R software used for further filtering and intersection, followed by the assessment of the relationship between these genes and NSCLC prognosis using log-rank tests and univariate Cox regression analysis. Meanwhile, six clinical NSCLC cancer and adjacent tissue samples were collected, along with the detection of TRIM58 mRNA and protein levels using RT-PCR and Western blot.</p><p><strong>Results: </strong>The study found that low expression of TRIM58 was significantly associated with poor prognosis in NSCLC patients, while experimental data suggested that the mRNA and protein expression levels of TRIM58 in NSCLC cancer tissues were significantly lower than those in adjacent normal tissues.</p><p><strong>Conclusion: </strong>This study indicates that low expression of TRIM58 may serve as a marker for poor prognosis in NSCLC patients. The low expression of TRIM58 and its promoter methylation state may be used for detecting minimal residual disease (MRD), providing new insights into early diagnosis and prognostic assessments and aiding in formulating individualized treatment strategies. Additionally, future research should increase the sample size and intensively explore the functional mechanisms of TRIM58 so as to validate its clinical application value.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"820-827"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Memoriam</i>: Frederick L. Kiechle, MD, PhD (1946-2024).","authors":"Charles D Hawker, Myra L Wilkerson","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"719-720"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of CXCR2 Deficiency in HeLa Cell on the Regulatory Network of Coding Genes and Non-Coding RNAs.","authors":"Wei Dong, Jifang Gao, Yilin Mu, Yanyan Wang, Xiaoyan Zhu, Jiayin Wang, Chen Zi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>C-X-C motif chemokine receptor 2 (CXCR2) plays a crucial role in inflammation and immunity, and the involvement of chemokine receptors in the tumor microenvironment is extensively documented. However, the impact of CXCR2 deficiency on the complete transcriptome, including mRNA and ncRNAs, in tumor cells remains unclear.</p><p><strong>Methods: </strong>In this study, we aimed to identify differentially expressed (DE) messenger RNA (mRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) in CXCR2 knockout HeLa cells through transcriptome sequencing and to construct regulatory networks.</p><p><strong>Results: </strong>Compared to control cells, differentially expressed mRNAs, lncRNAs and circRNAs, including 1306 coding genes, 397 lncRNAs, and 846 circRNAs were identified. Understanding these selected genes and ncRNAs could elucidate the influence of CXCR2 on tumor occurrence and progression. Furthermore, 6 DE genes, Rho guanine nucleotide exchange factor 4 (ARHGEF4), calcium voltage-gated channel auxiliary subunit alpha 2 delta 3 (CACNA2D3), fragile histidine triad (FHIT), potassium voltage-gated channel subfamily H member 5 (KCNH5), kelch like family member 1 (KLHL1) and teashirt zinc finger homeobox 2 (TSHZ2), 5 DE lncRNAs, CARMN, AL357060.1, AC098487.1, CECR7 and XLOC_009647, and 9 DE circRNAs, hsa_circ_0000196, hsa_circ_0000234, hsa_circ_0007976, hsa_circ_0008798, hsa_circ_0007766, hsa_circ_0116612, hsa_circ_0008012, hsa_circ_0004576 and hsa_circ_0118105 were verified using real-time PCR and sanger sequencing.</p><p><strong>Conclusion: </strong>Transcriptome analysis revealed that CXCR2 deficiency in HeLa cells alters the expression of coding genes and ncRNAs involved in tumor and cytokine signaling. Overall, our findings offer potential insights into the mechanisms and new research targets for cervical cancer and other tumors.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"799-809"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}