Annals of clinical and laboratory science最新文献

{"title":"Bergenin Suppresses Glycolysis and Malignant Progression in Breast Cancer via the IGF1R-MAPK Signaling Pathway.","authors":"Minhui Fan, Qingyu Li, Renya Shuai, Yunfei Shen, Fanglin Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>This study endeavors to unravel the effects and mechanisms of bergenin (Ber) in suppressing breast cancer (BC) progression through modulating the IGF1R-MAPK signaling pathway, using an integrative approach combining network pharmacology and bioinformatics.</p><p><strong>Methods: </strong>Ber-BC targets and related signaling pathways were systematically identified via network pharmacology and bioinformatics analyses. Key targets were further prioritized via machine learning algorithms, and their binding affinities with Ber were evaluated through molecular docking. IGF1R-overexpressing BC cell lines were subsequently established and pretreated with the MAPK inhibitor SB203580 for one hour before Ber administration. Functional assays were conducted to assess cellular proliferation, migration, glucose uptake, as well as intracellular pyruvate, lactate, and ATP. The extracellular acidification rate was derived via a Seahorse XF Analyzer. IGF1R expression was visualized via immunofluorescence. A murine xenograft model was generated through subcutaneous BC cell injection. Protein levels of HK2, PKM2, IGF1R, and phosphorylated p38 MAPK were quantified via western blotting, while immunohistochemistry was performed to evaluate Ki67 and IGF1R expression in tumor tissues.</p><p><strong>Results: </strong>Network pharmacology, bioinformatics, and MD identified eight pivotal targets (KDM5B, IGF1R, ADA, LGALS9, EGFR, SERPINE2, PHGDH, and ALOX5), among which IGF1R was prominently linked to the MAPK signaling pathway. <i>In vitro</i>, Ber markedly suppressed BC cell proliferation, migration, as well as glycolytic activity, concomitant with reduced IGF1R expression and diminished p38 MAPK phosphorylation. Rescue experiments proved that IGF1R overexpression notably ameliorated proliferation, migration, and glycolysis in Ber-treated BC cells, whereas SB203580 administration effectively reversed these effects. <i>In vivo</i>, Ber treatment significantly suppressed tumor growth and downregulated HK2, PKM2, and IGF1R protein expression, as well as p38 MAPK phosphorylation.</p><p><strong>Conclusion: </strong>Ber suppresses glycolysis and malignant progression in BC through modulation of the IGF1R-MAPK signaling pathway.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"214-226"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of CSF Biomarkers with Cognitive Screening Tests and PET Scan: A Tandem Approach.","authors":"Melody B Nelson, Corey Crecelius, Andrew P Jones, Candice Coffey, Amitava Dasgupta","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Testing for amyloid beta protein (Aβ42), total tau (t-Tau), and phosphorylated tau (p-Tau at position 181: p-Tau 181) in CSF using Roche Elecsys assays has been approved by the FDA as biomarkers for Alzheimer's disease. We studied correlation between pTau181/Aβ42 ratios (>0.023 positive cutoff), screening for cognitive functions (commonly used for screening patients suspected of dementia and AD) with actual AD diagnosis based on PET scans in 143 patients.</p><p><strong>Methods: </strong>All Roche Elecsys (electrochemiluminescence) assays for biomarkers were run on Cobas e 801 analyzer. Imaging studies were performed using fluorodeoxyglucose PET scans. Screening patients for cognitive functions (SCFs) included either MOCA, SLUMS, STMS, or MMSE.</p><p><strong>Results: </strong>Out of 143 patients with CSF AD biomarker ratios studied, 80 patients showed positive values (mean: 0.0555), while 63 patients showed negative ratios (mean: 0.0138). We also compared biomarker results with PET imaging in 40 patients (PET scan results were not available for all patients). Chi-square analysis showed a statistically significant association between positive CSF biomarker tests and PET scans {X²(1, N=40)=14.593; <i>p</i><0.01}. In contrast, we observed poor correlation between SCFs and PET scan results using chi-square analysis {X²(1, N=40)=3.558, <i>p</i>=0.059}. However, binary logistic regression demonstrated a combination of abnormal SCF and a positive CSF screen significantly predicted a positive imaging result {X²(2, N=40)=15.940, <i>p</i><.001}.</p><p><strong>Conclusions: </strong>CSF pTau181/Aβ42 ratios correlated significantly better with PET scan findings compared to various SCFs. Furthermore, a combination approach that included SCF and CSF biomarkers provided better correlation with positive PET imaging.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"139-143"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrence of Primary Mediastinal Yolk Sac Tumor with Brain Metastases: A Case Report and Literature Review.","authors":"Yan-Yan Zhu, Hang Yin, Chun-Li Xu, Li-Pei Wu, Yu Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Primary mediastinal yolk sac tumor (PMYST) is a rare and highly malignant germ cell tumor. We present a 24-year-old male admitted in 2018 with PMYST, superior vena cava syndrome (SVCS), and pulmonary and lymph node metastases. Following chemotherapy and partial resection, brain metastasis occurred; complete remission (CR) was achieved with chemotherapy plus cranial radiotherapy. Multiple bone metastases developed after 6 years. Re-resection revealed adenocarcinoma, consistent with chemotherapy-related malignant transformation. Despite multiple lines of chemotherapy and radiotherapy, the disease progressed, with an overall survival (OS) of 82 months. This case illustrates the complex clinical course of PMYST and the value of dynamic tumor marker and imaging surveillance.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"251-255"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>NFYA</i> Activates <i>REG1A</i> Expression to Promote Malignant Progression and Chemosensitivity in Rectal Cancer.","authors":"Xiaolu Zhai, Mei Hua, Ying Chen, Dian Yin","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the molecular mechanisms of rectal cancer (RC) progression and provide new insights for its treatment.</p><p><strong>Methods: </strong>The Cancer Genome Atlas (TCGA), Gene Expression Profiling Interactive Analysis (GEPIA), Tumor Immune Estimation Resource (TIMER) databases, and the UALCAN platform were used to predict gene expression and correlations. The JASPAR database was used to predict transcription factors. Quantitative polymerase chain reaction (qPCR) and western blot (WB) were performed to detect gene and protein expression. Cell viability was measured using the cell counting kit-8 (CCK-8). Transcription factor-promoter interactions were examined through chromatin immunoprecipitation (ChIP) and the dual-luciferase reporter assay (DLR assay). Transcription factor-gene interactions were validated <i>in vivo</i>. The stats and ggplot2 packages in R were used for analysis and visualization.</p><p><strong>Results: </strong>Regenerating islet-derived protein 1 alpha (<i>REG1A</i>) was significantly associated with RC malignant progression and was the most markedly upregulated. <i>REG1A</i> expression was elevated in RC patient tissues and cells. <i>REG1A</i> knockdown reduced RC cell survival and increased phosphorylated H2A histone family member X (<i>γ</i>H2AX) levels under X-ray or 5-Fluorouracil (5FU). Nuclear transcription factor Y subunit alpha (NFYA) was correlated with <i>REG1A</i>, and <i>NFYA</i> knockdown decreased <i>REG1A</i> expression and cell survival while increasing <i>γ</i>H2AX; <i>REG1A</i> reversed these effects. <i>In vivo</i>, <i>NFYA</i> knockdown reduced tumor volume, weight, and <i>REG1A</i> levels.</p><p><strong>Conclusion: </strong>NFYA promoted <i>REG1A</i> expression, and the <i>NFYA-REG1A</i> axis enhanced RC resistance to RT and CT and malignant progression.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"202-213"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Condyloma Detection in Neovagina Raises the Demand for Transgender Screening Guidelines. A Case Report and Review of the Literature.","authors":"Jessica Claus, Matty Clark, L Corey Hanley, Cara Mathews, M Ruhul Quddus","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Due to the progress in contemporary surgical methods and the increased awareness of gender dysphoria, vaginoplasty procedures have become more consistently standardized. In recent years, there has been a notable rise in gender-affirming surgeries, including breast reconstruction as well as genital modification procedures like hysterectomy, orchiectomy, and vaginoplasty. Current vaginoplasty techniques include penile inversion vaginoplasty, which uses penile and scrotal tissue, and intestinal vaginoplasty, which uses a segment of bowel to create a neovagina. Considering the tissues used for vaginoplasties, inversion vaginoplasties carry a potential risk of developing human papillomavirus (HPV)- related lesions, such as high-grade squamous intraepithelial lesions or squamous cell carcinomas. Similarly, intestinal vaginoplasties may be at risk of developing adenocarcinoma. Despite the occurrence of HPV-related lesions or malignancies in individuals with neovaginal structures, there is a lack of established screening guidelines for the suitable screening of neovagina-bearing transgender women and intersex patients.</p><p><strong>Case report: </strong>We report a case of a transgender woman in her late 20s, status post-inversion vaginoplasty, who presented with peri-anal and vaginal condylomas associated with pain and vaginal bleeding. Examination of the neovagina revealed a 9.0 cm friable mass. Partial excision as well as plasma jet ablation was performed and revealed a low-grade squamous vaginal intraepithelial lesion (VAIN 1).</p><p><strong>Conclusion: </strong>Due to the scarcity of reported cases, we would like to bring the need for routine screening for this patient group to reduce this substantial disparity in cancer screening for lesbian, gay, bisexual, transgender, and other sexual/ gender minority (LGBT +) patients.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"227-231"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Case Report of Familial Primary Hyperoxaluria Type 1 Nephropathy with c.781C>G Gene Mutation and Literature Review.","authors":"Anquan Liao, Wenjuan Duan, Wen Chen, Haiyang Zhou, Changrong Ye, Pingzhen Chen, WenJing Cai, Wanqian Wu, Hailang Wei, Guohui Liu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Primary hyperoxaluria type 1 (PH1), the most severe form of autosomal recessive hyperoxaluria, is caused by <i>AGXT</i> gene mutations that result in hepatic alanine-glyoxylate aminotransferase deficiency, which causes systemic oxalate accumulation and progressive nephropathy.</p><p><strong>Case report: </strong>The study reported a unique <i>AGXT</i> c.781C>G mutation in a family with two PH1 patients; the mutation was not previously documented in the literature. A 19-year-old male (Patient 1) with a long history of nephrolithiasis presented with end-stage renal disease (ESRD), hyperoxaluria, and significant metabolic derangements, requiring renal replacement therapy (RRT). Genetic testing revealed a homozygous <i>AGXT</i> c.781C>G mutation, confirming the diagnosis of PH1. His parents both were heterozygous carriers of the same mutation, and the 46-year-old father (Patient 2) exhibited similar manifestations, including nephrolithiasis and chronic renal insufficiency, with progression to ESRD during follow-up. PH1 exhibits significant clinical and genotypic heterogeneity, ranging from asymptomatic cases to recurrent or occasional stone formation, nephrocalcinosis, and ESRD, often leading to missed or delayed diagnosis. Genetic testing is the gold standard. Early diagnosis allows for interventions such as hydration, pyridoxine therapy, RNAi treatments, or liver transplantation to slow renal function decline, while advanced cases may require RRT or combined liver-kidney transplantation.</p><p><strong>Conclusions: </strong>This study firstly identified the <i>AGXT</i> c.781C>G mutation in PH1, expanding the known mutational spectrum of <i>AGXT</i>. The finding underscores the necessity of genetic screening in suspicious cases of familial nephrolithiasis or unexplained renal dysfunction.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"245-250"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the DNA Preservation Capacity of Two Methods for the Long-Term Cryopreservation of Whole Blood Samples.","authors":"Xiaoyu Wang, Rong Tang, Jingjing Liu, Ru Yin, Ping Zhu, Ling Zhu, Chunxia Zheng","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Comparative studies evaluating the quality of DNA from whole blood samples cryopreserved long-term in commercial PAXgene^® Blood DNA tubes versus anticoagulated all-cell pellet (ACP) samples are limited. Here, we assessed the yield, integrity, and purity of DNA from these two types of samples.</p><p><strong>Methods: </strong>We analyzed 100 randomly selected PAXgene^® samples (3 mL of blood processed per tube) that were stratified by storage duration into five-year (n=50) and 10-year (n=50) groups. In total, 50 randomly selected ethylenediaminetetraacetic acid (EDTA)-ACP samples that had been cryopreserved for 10 years were processed. DNA integrity was assessed using the DNA quality number (DQN), which was generated by a DNA fragment analyzer. DNA yield and purity were measured via spectrophotometry (NanoDrop). The sequencing eligibility criteria were a DNA yield ≥10 μg and a DQN ≥8.</p><p><strong>Results: </strong>The DNA extracted from the five-year and 10-year PAXgene^® tubes exhibited high-quality metrics (median yield: 111.60 μg vs. 85.32 μg; DQN: 9.45 vs. 9.30; A_260/280: 1.95 vs. 1.96; A_260/230: 2.14 vs. 2.17; all <i>P</i><0.05). Although the 10-year samples presented slightly lower DNA yields and quality, all PAXgene^® samples met the sequencing criteria. Compared with the 10-year PAXgene^® samples, the 10-year EDTA-ACP samples demonstrated comparable median yields (93.18 μg) and DQN values (9.40) but significantly lower purities (A_260/280: 1.83; A_260/230: 1.96) (<i>P</i><0.05). The sequencing pass rates were 92% for the EDTA-ACP samples and 100% for the PAXgene^® samples (<i>P</i>>0.05).</p><p><strong>Conclusions: </strong>After long-term cryopreservation, both methods provided DNA with sufficient yield and integrity for genomic studies. However, compared with the EDTA-ACP samples, the whole blood stored in the PAXgene^® Blood DNA tubes yielded DNA with higher purities and tended to have a higher sequencing pass rate.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"152-158"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Letter to the Editor:</i> Detection of <i>Chlamydia trachomatis</i> Immunoglobulin E Antibody in Human Sera.","authors":"Tamar A Smith-Norowitz, Haram Abdelmajid, Elliot Klein, Rauno Joks, Margaret R Hammerschlag, Stephan A Kohlhoff","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"256-258"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraoperative Imprint Cytology of <i>SMARCB1</i>-Mutant Desmoplastic Myxoid Tumor of the Pineal Region: A Case Report with Cytologic Differential Diagnosis and Literature Review.","authors":"Yukako Kinoshita, Xinhai R Zhang, Yoshua Esquenazi, Hidehiro Takei","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Desmoplastic myxoid tumor of the pineal region (DMTPR), <i>SMARCB1</i>-mutant, is a recently recognized and rare pineal region tumor with distinctive clinicopathologic features that contrast sharply with those of atypical teratoid/rhabdoid tumor, a well-known <i>SMARCB1</i>-deficient neoplasm. Although DMTPR generally exhibits low- to intermediate-grade histologic features and relatively indolent clinical behavior, its rarity and morphologic overlap with other pineal region neoplasms may pose significant diagnostic challenges, particularly during intraoperative consultation when tissue is limited.</p><p><strong>Case report: </strong>We report a case of DMTPR in a 38-year-old woman, with particular emphasis on its intraoperative touch imprint cytologic findings and their correlation with histologic features. The imprint preparations were composed of bland, relatively monomorphic oval-to-spindled tumor cells with delicate fibrillary processes embedded in a myxoid background. Small fibrillary acellular zones with variably collagenous areas were also identified, closely mirroring the tumor's characteristic myxoid to myxofibrillary histology with irregular anastomosing collagenous structures.</p><p><strong>Conclusion: </strong>This case expands the limited literature describing the cytologic features of DMTPR and highlights the value of touch imprint cytology as a useful adjunct to frozen-section evaluation. Recognition of its distinctive cytomorphologic features is important to avoid misclassification as other pineal region tumors during intraoperative assessment and to guide appropriate patient management.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"232-238"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network Pharmacology and <i>in vitro</i> Experimental Validation Reveal the Anticancer Mechanisms of Cichoric Acid in Cervical Cancer.","authors":"Jiajia Zhou, Qingyan Huang, Haikun Yang, Qinghua Liu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To systemically elucidate the multi-target anti-cervical cancer (CC) profile of Cichoric Acid (CA) and to provide both computational and experimental evidence for its clinical translation.</p><p><strong>Methods: </strong>A network-pharmacology pipeline integrating six databases was established to screen CA-CC common targets. PPI topology, GO/KEGG, survival, mutation, and immune-infiltration analyses were performed (cut-off: <i>p</i><0.05, FDR<0.05). Docking scores≤-5 kcal/mol were considered strong. Molecular dynamics simulations were performed to evaluate the structural stability of the <i>EGFR</i>- and <i>IL1B</i>-ligand complexes by analyzing RMSD trajectories. <i>In vitro</i>, the effects of CA on CC cell proliferation and migration were assessed in SiHa and CaSki cells using Cell Counting Kit-8 (CCK-8), wound healing, and Transwell assays. Anti-proliferation activity was evaluated in SiHa and CaSki cells by live-cell imaging and RT-qPCR (n=3 biologically independent repeats; one-way ANOVA followed by Dunnett's test).</p><p><strong>Results: </strong>CA yielded 87 putative targets, 86 of which overlapped with CC-related genes (hypergeometric <i>p</i>=2.3×10^-18). Topological analysis identified <i>IL1B</i> and <i>EGFR</i> as the top two hub genes (degree=28 and 26; betweenness≥0.015). High expression of either gene predicted worse five-year overall survival (<i>EGFR</i>: HR=1.85, 95 %CI: 1.16-2.96, <i>p</i>=0.0091; IL1B: HR=2.40, 95%CI: 1.5-3.82, <i>p</i>=0.00015) in 304 TCGA-CESC patients. Both genes positively correlated with M1-macrophage and neutrophil infiltration (Spearman r≥0.42, <i>p</i>≤0.002). Docking showed high affinity for <i>EGFR</i> (-6.12 kcal/mol) and IL1B (-5.71 kcal/mol). RMSD analysis demonstrated stable protein backbones and sustained ligand binding in both <i>EGFR</i>- and <i>IL1B</i>-ligand complexes throughout the simulations. <i>In vitro</i>, CA decreased cell number dose-dependently (IC50 312±14 μM in SiHa; 294±11 μM in CaSki) and reduced <i>EGFR</i> and <i>IL1B</i> mRNA by up to 68% and 71% at 600 μM (<i>p</i><0.001vscontrol). The CCK-8, wound healing, and Transwell assays consistently demonstrated that CA inhibited CC cell proliferation and migration <i>in vitro</i>.</p><p><strong>Conclusion: </strong>CA suppresses CC growth via concerted inhibition of <i>EGFR/IL1B</i> signaling and modulation of tumor immunity, warranting further pre-clinical and clinical investigation.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"182-201"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}