金边 Hb 病例显示不同的 HbA1c 水平取决于高效液相色谱系统。

IF 1.1 4区 医学 Q4 MEDICAL LABORATORY TECHNOLOGY
Masayuki Tojikubo, Takafumi Uchimura, Hidekazu Tamai, Masafumi Koga
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引用次数: 0

摘要

本报告介绍了一名 49 岁的金边 Hb 患者的病例,他的 HbA1c 水平随高效液相色谱(HPLC)系统的不同而变化。健康检查结果显示,高效液相色谱法测得的 HbA1c 水平高达 7.6%,而在当地一家诊所用免疫测定法测得的另一项 HbA1c 检测结果显示为 5.2%。鉴于不同检测方法的 HbA1c 水平存在差异,他来到我们诊所就诊。他的空腹血浆葡萄糖水平为 95 毫克/分升,但口服葡萄糖耐量试验显示他患有糖尿病。间歇扫描连续血糖监测(isCGM)的平均血浆葡萄糖水平为 117 mg/dL,估计 HbA1c 水平为 5.9%。根据间歇扫描连续血糖监测(isCGM)的结果,本病例被认为不是糖尿病。对同一标本进行了不同方法(系统)的 HbA1c 检测,包括使用 Tosoh G9 的 HPLC、使用 Arkray HA-8180 和 HA-8180T 的 HPLC、亲和力检测和酶法检测,结果显示 HbA1c 水平分别为 7.3%、4.9%、5.0%、5.4% 和 5.3%。经球蛋白基因分析,诊断为金边血红蛋白(α1-117Phe-Ile-α1-118Thr)。本病例有一个独特的血红蛋白变异体,使用 Tosoh 高效液相色谱系统测量时,HbA1c 水平较高,而使用 Arkray 高效液相色谱系统测量时,HbA1c 水平较低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Case of Hb Phnom Penh Showing Different HbA1c Levels Depending on the High-Performance Liquid Chromatography System.

The case of a 49-year-old man with Hb Phnom Penh whose HbA1c levels varied depending on the high-performance liquid chromatography (HPLC) system is presented. After the results of a health checkup showed a high HbA1c level of 7.6% measured by HPLC method, another HbA1c test measured by immunoassay at a local clinic showed a level of 5.2%. Given the discrepancy in HbA1c levels between assay methods, he visited our clinic. His fasting plasma glucose level was 95 mg/dL, but an oral glucose tolerance test showed diabetes mellitus. The mean plasma glucose level was 117 mg/dL on intermittently scanned continuous glucose monitoring (isCGM), giving an estimated HbA1c level of 5.9%. Based on the results of isCGM, the present case was considered to be not diabetes mellitus. The same specimen was assayed for HbA1c by various methods (systems), including HPLC with Tosoh G9, HPLC with Arkray HA-8180 and HA-8180T, affinity assay, and enzymatic assay, giving HbA1c levels of 7.3%, 4.9%, 5.0%, 5.4%, and 5.3%, respectively. A globin gene analysis diagnosed Hb Phnom Penh (α1-117Phe-Ile-α1-118Thr). The present case had a unique hemoglobin variant, resulting in high HbA1c levels when measured with Tosoh's HPLC systems and low HbA1c levels with Arkray's HPLC systems.

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来源期刊
Annals of clinical and laboratory science
Annals of clinical and laboratory science 医学-医学实验技术
CiteScore
1.60
自引率
0.00%
发文量
112
审稿时长
6-12 weeks
期刊介绍: The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.
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