Annals of clinical and laboratory science最新文献_第3页

Grading T Lymphocyte-Mediated Acute Interstitial Nephritis Following Checkpoint Inhibitor Therapy. 检查点抑制剂治疗后T淋巴细胞介导的急性间质性肾炎分级。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-03-01

Yazan A Al-Othman, Brandon D Metcalf, Olaf Kroneman, Jeffery M Gold, Sami Zarouk, Wei Li, Hassan D Kanaan, Ping L Zhang

{"title":"Grading T Lymphocyte-Mediated Acute Interstitial Nephritis Following Checkpoint Inhibitor Therapy.","authors":"Yazan A Al-Othman, Brandon D Metcalf, Olaf Kroneman, Jeffery M Gold, Sami Zarouk, Wei Li, Hassan D Kanaan, Ping L Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Immuno-checkpoint inhibitors (CPIs) such as PD-1/PD-L1 inhibitors have shown positive effects in treating various metastatic carcinomas but can cause complications like kidney dysfunction. This study aimed to determine if T lymphocytes were the dominant inflammatory cells in CPI-associated acute interstitial nephritis (AIN), graded using a modified Banff criteria for renal transplant cellular rejection.Methods: 20 renal biopsies from 18 patients with acute kidney injury following CPI treatment for metastatic carcinomas were evaluated. Infiltrating lymphocytes were stained for CD3 (T lymphocytes) and CD20 (B lymphocytes). AIN was graded using a modified Banff criteria for borderline changes and acute cellular rejection (ACR).Results: In 14 biopsies, typical AIN was dominated by CD3-positive T lymphocytes and a small percentage of B lymphocytes, with minimal eosinophils or plasma cells. There were one grade 3 AIN, four grade 2 AIN, and nine grade 1 AIN cases. Six biopsies without AIN showed either chronic thrombotic microangiopathy (TMA, n=1) or acute tubular injury (ATN, n=5). Following renal biopsies diagnosing AIN, nine out of 14 patients (64.3 %) experienced clinical improvement with steroid treatment.Conclusion: This study indicates that nephrotoxicity due to CPI treatment is characterized by T lymphocyte-mediated AIN, with the majority being grade 1 AIN according to the modified Banff criteria. Most patients showed some renal functional recovery in response to steroid treatment.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"172-178"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone Marrow Stromal Cells Promote Osteogenic Differentiation and Induce Bone Remodeling and Healing in Osteoporosis. 骨髓基质细胞促进骨质疏松症的成骨分化，诱导骨重塑和愈合。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-03-01

Xiaowei Jiang, Qiang Wang, Pu Ying, Wentao Jiang, Zhiyuan Qian, Wei Lu

{"title":"Bone Marrow Stromal Cells Promote Osteogenic Differentiation and Induce Bone Remodeling and Healing in Osteoporosis.","authors":"Xiaowei Jiang, Qiang Wang, Pu Ying, Wentao Jiang, Zhiyuan Qian, Wei Lu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Osteoporosis is a common disorder marked by bone loss, fragility, and fractures. microRNAs (miRNAs) are highlighted as potential regulators of osteoblast differentiation, and exosomes carrying miRNAs from bone marrow stromal cells (BMSCs) are applied to treatment. Herein, we aimed to clarify the role of miR-17 and BMSCs in ovariectomized rats with osteoporosis.Methods: After establishment of an osteoporosis animal model, miR-17 and BARX2 expression in ovariectomized rat tissues and osteoblasts were detected. Osteoblasts were transfected with mimics and inhibitor and co-cultured with BMSCs, whilst cell proliferation and differentiation were evaluated.Results: miR-17 was poorly expressed in ovariectomized rat tissue, while BARX2 was up-regulated. Overexpression of miR-17 induced decreased BARX2 expression but enhanced proliferation and differentiation of osteoblasts in osteoporotic rats. Importantly, the presence of BMSCs also facilitated osteoblast differentiation and decreased BARX2 expression, which was abolished by addition of exosome inhibitors. Mechanistically, miR-17 targeted BARX2 expression to up-regulate ALP and Co1-I in osteoporosis.Conclusion: Collectively, miR-17 delivered by BMSC exosomes promotes osteogenic differentiation and induces bone healing in osteoporosis through BARX2 regulation. The combined use of exosome inhibitors confirmed the therapeutic effect of BMSCs and BMSC exosomes containing miRNAs on various diseases.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"231-239"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Technical Note: Discordance Between Point of Care (POCT) BNP and Laboratory-Based NT-proBNP Values at Decision Cut-off in Some Patients - POCT BNP Values Below Cut-off Should be Interpreted with Caution. 技术说明：在一些患者中，护理点（POCT） BNP值与基于实验室的NT-proBNP值在决策临界值处不一致- POCT BNP值低于临界值应谨慎解释。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-01-01

Megan Nelson, Kristine Zinn, Mercedes Boyd, Amitava Dasgupta

{"title":"Technical Note: Discordance Between Point of Care (POCT) BNP and Laboratory-Based NT-proBNP Values at Decision Cut-off in Some Patients - POCT BNP Values Below Cut-off Should be Interpreted with Caution.","authors":"Megan Nelson, Kristine Zinn, Mercedes Boyd, Amitava Dasgupta","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Both B-type natriuretic peptide (BNP) and NT-pro BNP are markers for heart failure. In our hospital and other institutions, both point of care (POCT) BNP and clinical laboratory-based NT-Pro BNP test are used interchangeably by clinicians. We evaluated any discrepancy between these tests at decision cut-off point.Method: In a three-month period, in 120 patients, both POCT BNP and NT-proBNP were ordered at the same time and we evaluated these results.Results: We observed significant discordant results in 14 of these patients where POCT BNP concentrations were normal, but NT-pro BNP concentrations were elevated. Moreover, correlation between POCT BNP and NT-proBNP concentrations was also poor and in some cases NT-proBNP values were 15-fold higher than BNP.Conclusions: Because of this significant discordance, if both tests are ordered for initial diagnosis, POCT BNP result should be interpreted with caution if below the cut-off. Moreover, if only POCT BNP is ordered it may be normal in some patients with heart failure.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"142-144"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IL-10 Cytokine Responses in Chlamydia pneumoniae-Stimulated Human Peripheral Blood Mononuclear Cells. IL-10细胞因子在肺炎衣原体刺激的人外周血单核细胞中的反应。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-01-01

Tamar A Smith-Norowitz, Sarah Shidid, Nutchaya Amornruk, Haram Abdelmajid, Wefag Ahmed, Yitzchok M Norowitz, Stephan Kohlhoff

{"title":"IL-10 Cytokine Responses in Chlamydia pneumoniae-Stimulated Human Peripheral Blood Mononuclear Cells.","authors":"Tamar A Smith-Norowitz, Sarah Shidid, Nutchaya Amornruk, Haram Abdelmajid, Wefag Ahmed, Yitzchok M Norowitz, Stephan Kohlhoff","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Chlamydia pneumoniae (C. pneumoniae) is a gram-negative intracellular bacterium that causes respiratory infections and may contribute to inflammatory responses in asthma. Cell-mediated immune responses are important for protective immunity against C. pneumoniae; however, these responses may be impaired in asthma. Interleukin (IL)-10 is a cytokine that modulates innate and adaptive immunity. IL-10 is necessary for resolution of acute infection, and maturation of memory T cells. The aim of this study sought to compare IL-10 responses between subjects with or without asthma, and according to C. pneumoniae IgG antibody (Ab) or serum IgE status.Methods: Peripheral blood mononuclear cells (PBMC) (1×106/mL) from stable adult asthmatic (N=6) and non-asthmatic subjects (N=6) were infected +/- C. pneumoniae TW-183 at a multiplicity of infection (MOI)=0.1, using dose responses (1:10, 1:100), and cultured 48 hrs. IL-10 responses were measured in supernatants (ELISA). C. pneumoniae-IgG Abs and total serum IgE levels were measured in serum (EIA, ELISA).Results: Cytokine responses (mean differences: unstimulated-stimulated cells) were significant for IL-10 (1:10, 1:100) (-618±443, -1405±155) (P=0.0005, 0.0005) in all subjects (Wilcoxon signed-rank test). When subjects were stratified according to asthma, C. pneumoniae IgG Ab or serum IgE status, no significant cytokine responses were observed.Conclusions: C. pneumoniae stimulates IL-10 responses in subjects with or without asthma. These responses are not dependent on either asthma, C. pneumoniae IgG Ab or serum IgE status.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"79-83"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of miR-190a-3P on Chronic Diabetic Nephropathy by Regulating FGFR3. miR-190a-3P通过调节FGFR3对慢性糖尿病肾病的影响

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-01-01

Yuan Lin, Chaoyan Yin, Yanjing Liu, Donghui Lu, Xiaofen Lian

{"title":"Effect of miR-190a-3P on Chronic Diabetic Nephropathy by Regulating FGFR3.","authors":"Yuan Lin, Chaoyan Yin, Yanjing Liu, Donghui Lu, Xiaofen Lian","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Chronic diabetic nephropathy (CDN) is one of the common complications of the chronic diabetes. The FGF23/FGFR3-mediated signaling pathway is involved in CDN. Whether miR-190a-3P participates in CDN through regulation of FGFR3 remains to be elucidated. The present study evaluated miR-190a-3P's effect on CDN.Methods: Mice were divided into a control group (NC group), a CDN group, and CDN+miR-190a-3P antagonist group, followed by analysis of miR-190a-3P and FGFR3 level by qRT-PCR, FGFR3, and Tubulin protein level by Western blot as well as blood glucose, serum creatinine (Cr), and urea nitrogen (BUN) using an automatic biochemical analyzer.Results: Compared with the NC group, the CDN group had a significantly higher miR-190a-3P level in kidney tissue, while the FGFR3 mRNA level was lower (P<0.01). FGFR3 was a target gene of miR-190a-3P. miR-190a-3P levels in the CDN+miR-190a-3P antagonist group were significantly reduced compared with the CDN group (P<0.05). Meanwhile, FGFR3 protein levels in miR-190a-3P antagonist group were significantly increased (P<0.05). Compared with the NC group, the CDN group showed significantly reduced blood glucose level and elevated BUN and Cr level (P<0.01). However, CDN+miR-190a-3P antagonist group showed significantly increased blood glucose and reduced BUN and Cr level compared with CDN group (P<0.05).Conclusion: miR-190a-3P can directly bind to the 3'-UTR of FGFR3 mRNA and reduce FGFR3 protein levels, contributing to the occurrence of CDN.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"48-53"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression Profiles and Diagnostic Value of Serum Bile Acids in Intrahepatic Cholestasis of Pregnancy. 血清胆汁酸在妊娠肝内胆汁淤积症中的表达及诊断价值。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-01-01

Mengjie Xu, Kai Xu, Jun Li, Zibin Wang, Yujie Zhang, Tingting Yu

{"title":"Expression Profiles and Diagnostic Value of Serum Bile Acids in Intrahepatic Cholestasis of Pregnancy.","authors":"Mengjie Xu, Kai Xu, Jun Li, Zibin Wang, Yujie Zhang, Tingting Yu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: This study aimed to ascertain serum bile acid levels and explore the diagnostic value of differential bile acids in patients with intrahepatic cholestasis of pregnancy (ICP).Methods: 94 cases of ICP patients admitted to Wenzhou People's Hospital from Oct 2020 to July 2023 were selected as the ICP group, and 184 women who underwent normal prenatal examination in the same period as the healthy control group. The baseline data and 15 kinds of serum bile acid spectrum characteristics of the two groups of pregnant women were compared, and the risk factors of serum bile acid spectrum causing ICP were analyzed by univariate and multivariate logistic regression. A logistic regression model was constructed based on significant risk factors, and ROC curve was drawn to evaluate the diagnostic effect of the model.Results: Compared with the healthy control group, the other 14 serum bile acids except LCA were highly expressed in the ICP group, and there was significant difference (p<0.05). Univariate analysis showed that 15 serum bile acids were statistically different from ICP (p<0.05). Multivariate analysis showed that both TCA and DCA were risk factors for ICP, with 95% CI of 1.010 (1.003-1.021) and 1.003 (1.000-1.007), respectively. ROC curve showed that TCA, DCA, and TCA+ DCA were the diagnostic criteria of ICP, and their maximum diagnostic value was TCA+ DCA (AUC 0.932). The AUC values of TCA and DCA were 0.929 and 0.615, respectively. TCA+ DCA could play a complementary role in the diagnosis of ICP, and its sensitivity and specificity were 90.7% and 88.9%, respectively.Conclusion: TCA and DCA indicators are closely related to the occurrence of ICP, and the combined detection of TCA and DCA can significantly improve the efficiency of ICP diagnosis.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"72-78"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Consistency of Plasma Catecholamines and Metanephrines Analysis in China: A Comparison of Three Mainstream Commercially Available Liquid Chromatography Tandem Mass Spectrometry Kit Methods. 中国血浆儿茶酚胺和肾上腺素分析的一致性：三种主流市售液相色谱串联质谱试剂盒方法的比较

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-01-01

Nijiao Qin, Qingxiang Liu, Zhenni Liu, Ziyang Li, Binghui Li, Zhe Guo, Zhiyang Chen, Weiming Wu, Min Hu, Qichen Long

{"title":"The Consistency of Plasma Catecholamines and Metanephrines Analysis in China: A Comparison of Three Mainstream Commercially Available Liquid Chromatography Tandem Mass Spectrometry Kit Methods.","authors":"Nijiao Qin, Qingxiang Liu, Zhenni Liu, Ziyang Li, Binghui Li, Zhe Guo, Zhiyang Chen, Weiming Wu, Min Hu, Qichen Long","doi":"","DOIUrl":"","url":null,"abstract":"Objective: The consistency of plasma catecholamines and metanephrines measurements is crucial for the screening and diagnosis in pheochromocytomas and paragangliomas (PPGLs). There are many commercially available kits for plasma catecholamines and metanephrines testing, but their performance and consistency information are unknown. This study evaluated the performance of three mainstream commercially available LC-MS/MS kit methods and evaluated their consistency.Methods: Performance validation of three kit methods was performed according to Clinical Laboratory Standards Institute (CLSI) document C-62A. 40 plasma samples from patients tested for PPGLs were analyzed in three mainstream commercially available kit methods. Agreement of test results from different kit methods was assessed by Passing-Bablok regression and Bland-Altman analysis. Recalibration using uniform calibrations was performed to identify the possible calibration difference.Results: The performance of three mainstream commercially available LC-MS/MS kit methods was satisfied. The mean deviations ranges were as follows: epinephrine(E), -41.1% ~ -2.4%; norepinephrine (NE), -34.3% ~ -19.1%; metanephrine (MN), -6.5% ~ 0.3% and normetanephrine (NMN), -2.3% to 0.0%. After recalibration, the mean deviation ranges of the E and NE were reduced remarkably (E: -18% to -2.4%, NE: -19.1% to 8.0%).Conclusions: For MN and NMN, the consistency between the three commercially available kit methods is acceptable. The results for the E and NE showed unsatisfying comparability for almost all method pairs. The application of uniform calibrators could improve consistency among the mainstream systems for the measurement of plasma catecholamines.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"115-127"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The HbA1c-Adjusted Slow Valley Height Ratio, a Depth Indicator of the Valley Between HbA1c and HbA0 Measured by High-Performance Liquid Chromatography, Is Useful for the Diagnosis of Hb Toranomon. HbA1c调节慢谷高比是高效液相色谱法测定HbA1c与HbA0间谷的深度指标，对Hb异常的诊断有重要意义。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-01-01

Masafumi Koga, Aki Ishido, Yasushi Inoue, Yoshimi Imai, Hiroko Ijima, Hideaki Jinnouchi, Jun Murai, Mika Ito

{"title":"The HbA1c-Adjusted Slow Valley Height Ratio, a Depth Indicator of the Valley Between HbA1c and HbA0 Measured by High-Performance Liquid Chromatography, Is Useful for the Diagnosis of Hb Toranomon.","authors":"Masafumi Koga, Aki Ishido, Yasushi Inoue, Yoshimi Imai, Hiroko Ijima, Hideaki Jinnouchi, Jun Murai, Mika Ito","doi":"","DOIUrl":"","url":null,"abstract":"Objective: HbA1c values in patients with Hb Toranomon, a hemoglobin variant, are falsely high when measured with the Tosoh high-performance liquid chromatography (HPLC) analyzer. Moreover, a phenomenon has been observed in which the valley between HbA1c and HbA0 [slow valley (SV)] appears shallow on the chromatograms measured by the Tosoh HPLC analyzer in patients with Hb Toranomon. In the present study, the usefulness of the indicator, HbA1c (A1c)-adjusted SV height rate (AaSVHR), as a screening test for Hb Toranomon to quantify the SV height (SVH) was investigated.Methods: This study included six patients with Hb Toranomon and 100 control participants, comprising 50 diabetic patients and 50 non-diabetic participants. The SVH between HbA1c and HbA0 and the height of HbA1c (A1cH) on HPLC (G9) chromatograms were measured to determine the ratio of SVH to A1cH [SVH ratio (SVHR)]. Given that the SVHR obtained showed a significant negative correlation with HbA1c measured by HPLC, SVHR was corrected with HbA1c [A1c-adjusted SVHR (AaSVHR)], but it showed no significant correlation with HbA1c.Results: AaSVHR exceeded 14% across all participants with Hb Toranomon, but it was no more than 14% in all control participants.Conclusion: AaSVHR measurements are useful as an Hb Toranomon screening test in patients whose HbA1c measured with a Tosoh HPLC analyzer is falsely high. A high AaSVHR exceeding 14% raises a strong suspicion for the presence of a hemoglobin variant including Hb Toranomon.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"108-114"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Two SDS-Based Preparation Methods for Direct Identification and Antifungal Susceptibility Testing of Yeasts from Positive Blood Cultures. 评估两种基于 SDS 的制备方法，用于从阳性血培养物中直接鉴定酵母菌并进行抗真菌药敏试验。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-01-01

In Young Yoo, Hada Jang, Miran Lee, Hyun Soo Seok, Yeon-Joon Park

{"title":"Evaluation of Two SDS-Based Preparation Methods for Direct Identification and Antifungal Susceptibility Testing of Yeasts from Positive Blood Cultures.","authors":"In Young Yoo, Hada Jang, Miran Lee, Hyun Soo Seok, Yeon-Joon Park","doi":"","DOIUrl":"","url":null,"abstract":"Objective: We evaluated two SDS-based preparation methods for direct identification of yeasts using MALDI-TOF MS analysis and antifungal susceptibility testing (AFST).Methods: A total of 149 residual monomicrobial blood culture bottles (BCs) were included. For direct identification via MALDI-TOF MS analysis from positive BCs, two in-house methods with or without a 3-hr incubation step were evaluated. 55 samples were also prepared using a Sepsityper kit. In addition, to investigate the effects of differences in Candida spp. concentrations, simulated samples were incubated and colonies were counted. Direct AFST was performed using Sensititre YeastOne.Results: Correct identification rate was 65.8% for the saponin+0.5% SDS method and 70.5% for the 20% SDS after 3-hr incubation, respectively. For the 55 samples evaluated using the Sepsityper kit, both in-house methods showed significantly higher identification rates. For the simulated samples, Candida albicans had the lowest colony counts at the time of the positive signal in the BCs. Essential agreement was above 90% for all cases except for itraconazole using the pellet prepared by the 20% SDS method. Categorical agreement was above 90% for all cases.Conclusion: Our newly developed preparation method using 20% SDS demonstrated superior performance to a commercial kit, and can therefore be used for direct identification and AFST.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"102-107"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effect of Jiawei Shuxin Tang on Myocardial Ischemia-Reperfusion in Rats with Qi Deficiency and Blood Stasis and Its Correlation with NRG-1. 加味舒心汤对气虚血瘀大鼠心肌缺血再灌注的保护作用及其与NRG-1的相关性

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-01-01

Mingming Xu, Shen Wang, Dongming Lin

{"title":"Protective Effect of Jiawei Shuxin Tang on Myocardial Ischemia-Reperfusion in Rats with Qi Deficiency and Blood Stasis and Its Correlation with NRG-1.","authors":"Mingming Xu, Shen Wang, Dongming Lin","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the protective effect of Jiawei Shuxin Tang (JWSXT) on myocardial ischemia-reperfusion injury (MIRI) rats with Qi deficiency and blood stasis and its correlation with neuregulin-1 (NRG-1).Methods: 30 rats were used to establish the model of Qi deficiency and blood stasis, and were randomly divided into control group, MIRI model group, and JWSXT group. The traditional Chinese medicine (TCM) phenotypes, echocardiographic performance, myocardial reperfusion area, myocardial infarction area, HE staining, and NRG-1 content changes of rats in each group were compared one week after treatment.Results: We found that compared with the model group, rats in the JWSXT group showed increased activity as well as improved tongue texture and lip color (P<0.05); LVEF and LVFS increased in the JWSXT group (P<0.05; P<0.05). The percentage of infarct area and the percentage of myocardial ischemia reperfusion area in the JWSXT group was significantly reduced (P<0.05; P<0.05). Compared with the control group, the remaining NRG-1 content in myocardial tissues of rats in the two groups was decreased (P<0.01), and NRG-1 content in the JWSXT group was increased (P<0.05) compared with the model group.Conclusions: JWSXT can improve reperfusion injury in rats with Qi deficiency and blood stasis infarction, which correlates with NRG-1.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"84-95"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0