Annals of clinical and laboratory science最新文献_第3页

STCB Inhibits HepG2 Cell Proliferation by Regulating Ras Signaling and Autophagy. STCB通过调控Ras信号和自噬抑制HepG2细胞增殖。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2026-01-01

Hao-Liang Ke, Zhen Zhou, Wang Ai, Lu Peng, Xiu-Ping Wang, Ying-Wen Zhang

{"title":"STCB Inhibits HepG2 Cell Proliferation by Regulating Ras Signaling and Autophagy.","authors":"Hao-Liang Ke, Zhen Zhou, Wang Ai, Lu Peng, Xiu-Ping Wang, Ying-Wen Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: The role of traditional Chinese medicine in cancer treatment is becoming increasingly pivotal. Tupistra chinensis Baker (STCB), a member of the Lily family, is an herbal plant with antipyretic and detoxifying properties. It has also been used to alleviate abdominal distension. Previous studies have shown that saponin extracted from STCB inhibits proliferation of hepatic carcinoma (HCC) cells. The present study aimed to explore the mechanisms implicated in the regulation of HCC progression by STCB.Methods: The impact of STCB on HepG2 hepatoblastoma cell proliferation in vitro was evaluated using Cell Counting Kit-8 assay. The levels of Ras signaling-related and autophagy-related proteins in cancer cells and tumor tissues were determined using Western blot, immunohistochemistry, and immunofluorescence assays.Results: STCB significantly inhibited cell growth similar to Salirasib in vitro. STCB combined with Salirasib resulted in a higher inhibition rate of HepG2 cell growth. STCB treatment also suppressed Ras expression and phosphorylation of its downstream proteins. STCB treatment also upregulated levels of autophagy-related proteins. In mouse xenograft models, STCB impeded growth of subcutaneously transplanted hepatic tumors as well as inhibited Ras signaling, similar to cisplatin treatment.Conclusion: STCB significantly inhibited HepG2 cell proliferation by downregulating Ras signaling-related proteins and inducing autophagy.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 1","pages":"42-50"},"PeriodicalIF":1.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147607784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Implication of Alterations in Serum Klotho Levels in Type 2 Diabetes Mellitus with Differential C-Peptide Patterns. 2型糖尿病血清Klotho水平变化与不同c肽模式的临床意义

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-11-01

Xiaohong Luo, Dongqing Zhang, Xuexuan Zhang, Tianming Liu, Yinge Wu

{"title":"Clinical Implication of Alterations in Serum Klotho Levels in Type 2 Diabetes Mellitus with Differential C-Peptide Patterns.","authors":"Xiaohong Luo, Dongqing Zhang, Xuexuan Zhang, Tianming Liu, Yinge Wu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Klotho is relevant to glucose and lipid metabolism and may be involved in insulin resistance (IR), which is a hallmark of type 2 diabetes mellitus (T2DM). This study aimed to elucidate the alterations in serum Klotho levels and their relationship with IR in T2DM with varying C-peptide patterns.Methods: 690 T2DM subjects with different levels of serum C-peptide were enrolled. Blood samples were analyzed for FPG, HbA1c, C-peptide, FINS, and Klotho. Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) was calculated as HOMA-IR=FINS (μU/ml)×FPG (mmol/L)/22.5. Finally, receiver operating characteristic (ROC) analysis was used to explore the diagnostic performance based on β-cell function in T2DM.Results: Klotho levels were significantly lower in T2DM patients compared to in controls, with decreasing maximum in the hyper-C-peptidemia group, while the HOMA-IR exhibited an inverse tendency across T2DM subgroups. In addition, Klotho was inversely correlated with HOMA-IR, FINS, FPG, and HbA1c, especially in T2DM with hyper-C-peptidemia. Moreover, the relationship remained significant even after additional adjustment for FGF23 and eGFR. Finally, Klotho displayed a good ability in distinguishing T2DM patients with different C-peptide patterns from controls, although its performance was marginal among T2DM subgroups.Conclusion: In T2DM, serum Klotho levels were negatively correlated with HOMA-IR, and Klotho showed a good ability in distinguishing T2DM patients with differential C-peptide level patterns from healthy controls.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 6","pages":"954-963"},"PeriodicalIF":1.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship of Serum Creatinine Level at Admission to an ICU for Cardiac Arrest with Subsequent Acute Kidney Injury. 心脏骤停患者入院时血清肌酐水平与随后急性肾损伤的关系。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-11-01

Wan-Wan Zou, Qi-Kun Zhu, Song Yang, En-Ze Bian, Jian Zhuang, Jian-Zheng Cen, Yun Teng, Qiang Gao

{"title":"Relationship of Serum Creatinine Level at Admission to an ICU for Cardiac Arrest with Subsequent Acute Kidney Injury.","authors":"Wan-Wan Zou, Qi-Kun Zhu, Song Yang, En-Ze Bian, Jian Zhuang, Jian-Zheng Cen, Yun Teng, Qiang Gao","doi":"","DOIUrl":"","url":null,"abstract":"Objective: We investigated the association between the serum creatinine (SCr) level at admission to an intensive care unit (ICU) for cardiac arrest (CA) and subsequent acute kidney injury (AKI).Methods: Data were from a previous study that was conducted at a single secondary care institute. Patients were included if they received successful cardiopulmonary resuscitation (CPR) after in-hospital CA (IHCA) or out-of-hospital CA (OHCA) and were admitted to an ICU. Patients were excluded if their SCr level at admission was missing or if they were younger than 18 years old. A total of 435 patients were screened for eligibility. 61 patients were excluded and the records of 374 patients who met the inclusion and exclusion criteria were examined.Results: Two multivariate models that adjusted for confounding factors showed that the SCr level at admission was independently associated with AKI in patients who received successful CPR. Receiver operating characteristic (ROC) analysis showed that an increased SCr level at admission was associated with subsequent AKI (area under the curve [AUC]: 0.823, 95% CI: 0.781, 0.865), and that the optimal SCr cutoff level was 1.15 mg/dL.Conclusion: An increased SCr level at ICU admission for CA was independently associated with subsequent AKI without sex-related discrepancies, and the optimal cutoff level was 1.15 mg/dL.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 6","pages":"879-887"},"PeriodicalIF":1.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentiation-Inducing Effects of Triciribine and All-Trans Retinoic Acid in Acute Myeloid Leukemia Cell lines. 曲西瑞滨和全反式维甲酸对急性髓系白血病细胞的诱导分化作用。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-11-01

Yuri Sato-Nagaoka, Shinichiro Takahashi

{"title":"Differentiation-Inducing Effects of Triciribine and All-Trans Retinoic Acid in Acute Myeloid Leukemia Cell lines.","authors":"Yuri Sato-Nagaoka, Shinichiro Takahashi","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the differentiation-inducing activity of triciribine (TCN), alone and in combination with all-trans retinoic acid (ATRA), in acute promyelocytic leukemia (APL) and acute myeloid leukemia (AML) cell lines, including ATRA-resistant HL-60-R2.Methods: APL (NB4) and AML (HL-60, K052, HL-60-R2) cell lines were treated with TCN and/or ATRA. Cell proliferation was measured using Cell Count Reagent SF. Differentiation was assessed by quantitative PCR, flow cytometry for surface markers, morphological analysis with Wright-Giemsa staining and nuclear-to-cytoplasmic ratio quantification, and nonspecific esterase staining.Results: TCN and ATRA each inhibited proliferation, with combination treatment producing a significant additive effect. Expression of differentiation markers, particularly CD11b and CD11c, was enhanced under combined treatment. Morphological analyses revealed cytoplasmic expansion, nuclear indentation, peripheral budding, and vacuole formation, with significantly reduced nuclear-to-cytoplasmic ratios. Nonspecific esterase staining confirmed α-naphthyl butyrate esterase positivity, supporting induction of partial myelomonocytic differentiation in all cell lines, including ATRA-resistant HL-60-R2.Conclusion: TCN augments the differentiation-inducing activity of ATRA and promotes partial myelomonocytic differentiation, even in ATRA-resistant AML cells. Combination of TCN with established differentiation therapy may provide a strategy to overcome differentiation blockade in AML.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 6","pages":"869-878"},"PeriodicalIF":1.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatal Eosinophilic Pulmonary Vasculitis in a Neonate - The First Documented Case in the English Literature. 新生儿致死性嗜酸性肺血管炎-英文文献中第一例。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-11-01

Jessica Claus, Robert Barno, Joseph M Bliss, Christine Yu, Monique DePaepe

{"title":"Fatal Eosinophilic Pulmonary Vasculitis in a Neonate - The First Documented Case in the English Literature.","authors":"Jessica Claus, Robert Barno, Joseph M Bliss, Christine Yu, Monique DePaepe","doi":"","DOIUrl":"","url":null,"abstract":"Objective: This case presentation offers an in-depth exploration of the complex and multifaceted realm of neonatal eosinophilic vasculitis, an extremely rare entity that has not been reported in the English literature. Only one similar case has been described in an Italian publication in 1995. Our case places an emphasis on the clinical presentation, postmortem findings, and evaluation of the importance of a multidisciplinary approach to this enigmatic disease.Case report: We report a case of a 33-week neonate born via cesarean section following premature preterm rupture of membranes who died of an unknown etiology within the first 30 minutes of life despite maximal resuscitation efforts. A limited postmortem examination of only the lungs and heart was requested and revealed a necrotizing eosinophilic vasculitis of the lungs; these histopathologic findings are extremely uncommon in the neonate population.Conclusion: In other patient groups, eosinophilic vasculitis is a hallmark of several interstitial lung diseases. The etiology widely ranges from viral, fungal, parasitic infections, allergic reactions, hypereosinophilic syndrome, and various vasculitis types such as eosinophilic granulomatosis with polyangiitis (formerly Churg-Strauss syndrome) and granulomatosis with polyangiitis. The potentially fatal outcome for neonates and the lack of a clear etiology identified in the neonatal population necessitates urgent attention to this rare condition.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 6","pages":"972-976"},"PeriodicalIF":1.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomics for Sudden Cardiac Death: A Review of the Topic and Call for Increased Professionalism in Clinico-Molecular Autopsy and Forensic Laboratory Sciences. 心源性猝死的基因组学：对该主题的回顾以及对临床分子解剖和法医实验室科学专业水平提高的呼吁。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-11-01

Ivan Šoša, Consolato M Sergi

{"title":"Genomics for Sudden Cardiac Death: A Review of the Topic and Call for Increased Professionalism in Clinico-Molecular Autopsy and Forensic Laboratory Sciences.","authors":"Ivan Šoša, Consolato M Sergi","doi":"","DOIUrl":"","url":null,"abstract":"In 2001, the first draft sequence of the human genome was released, the culmination of a decade-long, multibillion-dollar effort. Since then, an OMICs platform has been proposed to further evaluate and edit the human genome for diagnostic and therapeutic purposes. The Human Genome Project opened a Pandora's box, expanding the forensic laboratory physicians' toolbox. Kinship analysis has been used extensively for parentage testing and identifying cases of human remains and missing persons. The Combined DNA Index System has played a significant role in forensic DNA databases. Nanopore sequencing and improved genomic tools aid enormously in identifying amplicons from degraded samples. The application of genomics in determining the potential channelopathies of sudden cardiac death (SCD) has been an enormous step forward in recent years in forensic histopathology. We reviewed the literature. Kong et al.'s meta-analysis of the mean allele frequencies of SCN5A, NOS1AP, KCNH2, KCNE1, and KCNQ1 genes across Black, Caucasian, Asian, and Hispanic ethnicities has been pivotal to forensic science in the last decade. The authors used sequenced genomic data from the Exome Aggregation Consortium to compare allele frequencies between different ethnicities. They found that Asians had the highest overall mean allele frequencies for NOS1AP and SCN5A, Caucasians had the highest KCNH2 frequency, and Hispanics had the highest KCNQ1 frequency. In 2026, the call for increased professionalism in clinico-molecular autopsy and forensic laboratory sciences is driven by rapid technological advancements (e.g., forensic molecular genomic autopsies), critical workforce shortages in some geographical areas, and the increasing complexity of death investigations. This professionalization focuses on standardizing molecular protocols, enhancing ethical frameworks, and addressing the need for specialized interdisciplinary expertise.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 6","pages":"859-868"},"PeriodicalIF":1.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marine Microbial Ectoine Mitigates Lipoglucotoxic Injury in H9c2 Cardiomyocytes and Activates AMPK. 海洋微生物外托碱减轻H9c2心肌细胞脂糖毒性损伤并激活AMPK。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-11-01

Yu Zhang, Guangju Zhou

{"title":"Marine Microbial Ectoine Mitigates Lipoglucotoxic Injury in H9c2 Cardiomyocytes and Activates AMPK.","authors":"Yu Zhang, Guangju Zhou","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Ectoine is a natural compatible solute known for its cytoprotective properties, though its potential role in alleviating diabetic cardiomyopathy remains largely unexplored. This study aimed to examine the protective effects and underlying mechanisms of Ectoine on H9c2 cardiomyocytes under high-glucose and high-palmitic acid conditions.Methods: The investigation included assessment of cell viability and lactate dehydrogenase release. Oxidative stress was evaluated through measurements of reactive oxygen species, malondialdehyde levels, and superoxide dismutase activity. Inflammatory responses were analyzed via TNF-α secretion and NF-κB and ERK1/2 pathways. Molecular docking studies were conducted to explore Ectoine-AMPK interaction, while mechanistic insights were gained through examination of AMPK signaling, energy metabolism regulators, autophagic flux markers, and mitochondrial apoptosis.Results: Ectoine treatment was associated with enhanced cell viability and reduced lactate dehydrogenase release. It appeared to alleviate oxidative stress through decreased reactive oxygen species and malondialdehyde levels, along with increased superoxide dismutase activity. Ectoine also showed suppressive effects on inflammatory responses, including reduced TNF-α secretion and inhibited NF-κB and ERK1/2 pathways. Molecular docking suggested binding affinity between Ectoine and AMPK (-6.4 kcal/mol). Mechanistically, Ectoine demonstrated potential in activating AMPK signaling, modulating energy metabolism, restoring autophagic flux, and attenuating mitochondrial apoptosis.Conclusion: The findings suggest that Ectoine may protect against HG-PA-induced cardiomyocyte injury. This protective effect was associated with AMPK activation and the coordinated modulation of metabolic, autophagic, anti-inflammatory, and anti-apoptotic pathways. This study suggests the therapeutic potential of Ectoine and provides a basis for further investigation.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 6","pages":"939-953"},"PeriodicalIF":1.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Concurrent Elevation of CEA and CA 19-9 in a Patient with Allergic Bronchopulmonary Aspergillosis: A Case Report and Literature Review. 过敏性支气管肺曲菌病患者CEA和ca19 -9同时升高1例报告并文献复习。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-11-01

Changxiao Li, Fangyuan Zheng, Mingzhi Jiao, Mei Qi, Jiling Lv

{"title":"Concurrent Elevation of CEA and CA 19-9 in a Patient with Allergic Bronchopulmonary Aspergillosis: A Case Report and Literature Review.","authors":"Changxiao Li, Fangyuan Zheng, Mingzhi Jiao, Mei Qi, Jiling Lv","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Serum carbohydrate antigen (CA 19-9) and carcinoembryonic antigen (CEA) are common tumor markers that are mainly used in the diagnosis and therapeutic evaluation of malignant tumors. Here, we detail a case of allergic bronchopulmonary aspergillosis (ABPA) presenting with elevation of CEA and CA 19-9 levels.Case report: A 22-year-old non-asthmatic male presented with recurrent cough, white mucopurulent sputum, and elevated serum CEA (20.55 ng/mL) and CA 19-9 (>1000 U/mL) levels. Investigations revealed Aspergillus-positive sputum culture, central bronchiectasis, mucus plugs on CT, eosinophilia, and migratory pulmonary lesions. Initial voriconazole therapy failed to prevent relapses. ABPA diagnosis was confirmed after re-evaluation. Combined voriconazole and methylprednisolone treatment achieved sustained remission over three years with normalization of tumor markers.Results: Elevation of CEA and CA 19-9 levels correlated with ABPA disease activity but not IgE/eosinophil levels. Post-treatment normalization of CEA and CA 19-9 levels supports their utility as inflammatory biomarkers. This case expands the ABPA phenotype and cautions against misinterpreting tumor markers as malignancy indicators in benign inflammatory contexts.Conclusion: ABPA should be considered in non-asthmatic patients with recurrent respiratory symptoms and elevated tumor markers. Serial monitoring of CEA/CA 19-9 may aid therapeutic assessment.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 6","pages":"977-980"},"PeriodicalIF":1.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annals of Clinical and Laboratory Science: Information for Authors. 临床和实验室科学年鉴：作者信息。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-11-01

引用次数: 0

The Predictive Value of Subclinical Hypothyroidism in Coronary Artery Lesions in Patients with Coronary Heart Disease. 亚临床甲状腺功能减退对冠心病患者冠状动脉病变的预测价值。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-11-01

Tingting He, Peiyou Yang, Jin Ni, Yu Liu, Na Niu, Junpeng Ma, Yang Jiao, Tielong Fan

{"title":"The Predictive Value of Subclinical Hypothyroidism in Coronary Artery Lesions in Patients with Coronary Heart Disease.","authors":"Tingting He, Peiyou Yang, Jin Ni, Yu Liu, Na Niu, Junpeng Ma, Yang Jiao, Tielong Fan","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Subclinical hypothyroidism (SCH) is associated with an increased risk of coronary heart disease (CHD), yet its predictive value for the severity of coronary artery lesions remains unclear. This study aimed to investigate the relationship between SCH and the Gensini score, a quantitative measure of coronary artery lesion severity, in patients with CHD.Methods: A retrospective single-center study was conducted involving 311 patients with CHD confirmed by coronary angiography, including 166 with SCH (TSH>4.5 mIU/L with normal FT3/FT4) and 165 euthyroid controls. The Gensini score was assessed by blinded cardiologists. Thyroid function parameters (TSH, FT3, FT4) and traditional cardiovascular risk factors were analyzed. Statistical analyses included Spearman correlation, multiple linear regression, and ROC curve analysis.Results: Patients with SCH had significantly higher median Gensini scores than controls (46 vs. 15, P<0.0001). TSH was strongly positively correlated with the Gensini score (ρ=0.837, P<0.0001). Multiple regression confirmed that SCH independently predicted higher Gensini scores after adjusting for traditional risk factors (β=0.168, P<0.001). ROC analysis showed that SCH status alone had an AUC of 0.968 for predicting high-risk lesions (Gensini ≥40), with an optimal TSH cutoff of >5.8 mIU/L (sensitivity 85%, specificity 83%). The combined model including SCH and traditional factors further improved predictive performance (AUC=0.906, NRI=17.3%, P=0.002).Conclusion: SCH is independently associated with more severe coronary artery lesions in CHD patients, as quantified by the Gensini score. TSH>5.8 mIU/L may serve as a valuable threshold for identifying high-risk patients. Incorporating thyroid function screening into CHD risk assessment could enhance stratification and guide early intervention.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 6","pages":"898-905"},"PeriodicalIF":1.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0