Association of MMP-2 Variant with Atrial Fibrillation in Elderly Patients: A Comparative Analysis Involving GJA1 and IL-6R Variants.

IF 1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Annals of clinical and laboratory science Pub Date : 2025-05-01

Akile Tuncal, Hikmet Hakan Aydın, Elif Karadadas, Levent Hurkan Can, Emre Demir, Handan Ak

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引用次数: 0

Abstract

Objective: Atrial fibrillation (AF) is a common arrhythmia caused by genetic modifications affecting biological processes such as cell communication, inflammation, and ion channels. Changes that occur over time happen gradually. By understanding the functional significance of these variations, we have analyzed single nucleotide polymorphisms (SNPs) in matrix metalloproteinase 2 (MMP-2), connexin 43 (GJA1/CX43), and interleukin 6 receptor (IL-6R) in patients aged 65 or older who have been diagnosed with atrial fibrillation. This analysis is grounded in the biological processes that are related to AF.

Methods: Blood samples were collected from 301 individuals, consisting of 151 patients (77 males and 74 females) and 150 control subjects (93 males and 57 females), aged between 65 and 80. We employed a TaqMan assay, a PCR-based genotyping technique, to examine samples for the presence of three specific SNPs: rs243865 in the MMP-2 gene, rs13216675 in the GJA1/CX43 gene, and rs4845625 in the IL-6R gene.

Results: Upon comparing the patient group to the control group, a notable contrast was found in MMP-2 variations between the reference allele (C) and alternate allele (T) (p=0.0053). Individuals with the TT homozygous genotype exhibited a higher odds ratio (4.57, p=0.02).

Conclusion: A significant association has been observed between rs243865 in the MMP-2 gene, highlighting its crucial role in the pathways related to the association and development of AF. However, no significant difference was found between the control group and patients regarding the GJA1 and IL-6R genetic variations.

本刊更多论文

老年患者心房颤动与MMP-2变异的关系：涉及GJA1和IL-6R变异的比较分析

目的：心房颤动（AF）是一种常见的心律失常，由影响细胞通讯、炎症和离子通道等生物过程的遗传修饰引起。随着时间的推移，变化是逐渐发生的。通过了解这些变异的功能意义，我们分析了65岁及以上房颤患者基质金属蛋白酶2 （MMP-2）、连接蛋白43 （GJA1/CX43）和白细胞介素6受体（IL-6R）的单核苷酸多态性（snp）。方法：采集301人的血液样本，包括151例患者（男性77例，女性74例）和150例对照组（男性93例，女性57例），年龄在65 - 80岁之间。我们采用TaqMan法（一种基于pcr的基因分型技术）检测样本中是否存在三个特定的snp: MMP-2基因中的rs243865、GJA1/CX43基因中的rs13216675和IL-6R基因中的rs4845625。结果：患者组与对照组比较，参比等位基因(C)与交替等位基因(T)的MMP-2变异有显著性差异（p=0.0053）。TT纯合子基因型个体的优势比更高（4.57,p=0.02）。结论：MMP-2基因rs243865存在显著相关性，在AF相关通路中发挥重要作用，但GJA1和IL-6R基因变异在对照组和患者之间无显著差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of clinical and laboratory science 医学-医学实验技术

CiteScore

1.60

自引率

0.00%

发文量

112

审稿时长

6-12 weeks

期刊介绍： The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.