Animal Nutrition最新文献

{"title":"Metabolic and muscular adaptations to dietary succinate in Tan sheep: Insights from transcriptomics","authors":"Wenyi Ren, Ning Zhang, Yuchen Cheng, Shuangming Yang, Xiaonv Liu, Miao Liu, Weihao Kong, Xiaofeng Xu, Lili Zhang","doi":"10.1016/j.aninu.2026.01.017","DOIUrl":"https://doi.org/10.1016/j.aninu.2026.01.017","url":null,"abstract":"","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"267 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148384497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diammonium phosphate supplementation in low-protein diets improves nitrogen utilization and feed efficiency without impairing carcass traits or meat quality in finishing pigs","authors":"Xianglin Zeng, Yan Tang, Siqi Li, Qiye Wang, Huansheng Yang","doi":"10.1016/j.aninu.2025.12.021","DOIUrl":"https://doi.org/10.1016/j.aninu.2025.12.021","url":null,"abstract":"","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"21 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148384493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation with raw potato starch enhanced tibia mass of meat ducks potentially linked to intestine-derived inflammation","authors":"Jixue Yin, Xin Yue, Simeng Qin, Yuying Huang, Minh Tu Nguyen, Herinda Pertiwi, Yanqun Huang, Rui Zheng, Wen Chen, Huaiyong Zhang","doi":"10.1016/j.aninu.2025.08.017","DOIUrl":"https://doi.org/10.1016/j.aninu.2025.08.017","url":null,"abstract":"","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"41 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148383906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-starch diet promotes growth and slaughter performance in Holstein bulls while enhancing intramuscular fat deposition and improving meat quality","authors":"Haoqi Han, Chunxiao Dong, Liqin Ma, Yanjun Shen, Weixuan Tang, Xiaojing Liu, Yangyi Hao, Wei Wang, Yanfen Ma, Shengli Li","doi":"10.1016/j.aninu.2026.02.008","DOIUrl":"https://doi.org/10.1016/j.aninu.2026.02.008","url":null,"abstract":"Owing to the increasing global demand for high-quality beef, Holstein bulls are an important beef production resource despite their inferior meat quality. As high-starch diets can enhance intramuscular fat deposition and improve meat quality, the effects of varying starch levels on growth, serum biochemical indicators, rumen fermentation, and slaughter performance including meat quality, and amino acid (AA) and fatty acid (FA) profiles in the longissimus lumborum muscle of finishing Holstein bulls were evaluated. In a randomized block design, 45 fifteen-month-old Holstein bulls (initial body weight: 501.5 ± 4.86 kg) were allocated to three dietary treatments containing 35% (35S), 40% (40S), or 45% (45S) starch in a randomized block design. Each treatment consisted of 15 bulls, organized into three replicates per group with five bulls per replicate. The feeding trial lasted 3 months prior to slaughter (six bulls per group were selected for slaughter evaluation). Average daily gain of dairy bulls in the 40S group tended to be higher than that in the 45S group (<ce:italic>P</ce:italic> = 0.093), and dry matter intake linearly decreased with increasing dietary starch level (<ce:italic>P</ce:italic> = 0.044). Serum malondialdehyde (<ce:italic>P</ce:italic> = 0.075), lipopolysaccharide (<ce:italic>P</ce:italic> = 0.012), and tumor necrosis factor-α (<ce:italic>P</ce:italic> = 0.022) increased linearly with increasing dietary starch level. Rumen pH decreased linearly (<ce:italic>P</ce:italic> &lt; 0.001) with increasing starch level, whereas propionate (<ce:italic>P</ce:italic> = 0.005), butyrate (<ce:italic>P</ce:italic> = 0.003), and total volatile FA (<ce:italic>P</ce:italic> = 0.030) increased linearly. The 40S group exhibited higher carcass yield (<ce:italic>P</ce:italic> = 0.027) and larger ribeye area (<ce:italic>P</ce:italic> = 0.036) than both 35S and 45S groups. The intramuscular fat content and lipid droplet area were similar between the 35S and 40S groups, but greater in the 40S group than in the 45S group (<ce:italic>P</ce:italic> = 0.048). The shear force was lower in 35S and 40S groups than that in 45S group (<ce:italic>P</ce:italic> = 0.010). Both 35S and 40S groups also had altered AA profiles and higher saturated, monounsaturated, and total FA contents relative to 45S group (<ce:italic>P</ce:italic> &lt; 0.05). Thus, 35%-40% starch diets improved growth and slaughter performance in Holstein bulls while enhancing rumen fermentation status and promoting IMF deposition for better meat quality.","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"8 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148356523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardized ileal digestible linoleic acid requirement of modern lactating sows: A dose–response study","authors":"Yuansen Yang, Yifan Chen, Wenjun Gao, Yu Wei, Nuo Xiao, Xiaokang Zhang, Lu Wang, Changhua Lai","doi":"10.1016/j.aninu.2026.03.003","DOIUrl":"https://doi.org/10.1016/j.aninu.2026.03.003","url":null,"abstract":"Continued genetics and management improvement necessitates the verification of nutrient requirements for prolific and high-producing modern sow. Therefore, the objective of this study was to determine the standardized ileal digestible (SID) linoleic acid (LA) requirement of lactating sows using dose-response estimation methods. A total of 100 healthy Yorkshire × Landrace sows (parity two to four; initial body weight 245.0 ± 15.8 kg) were randomly assigned to five treatment groups with 20 replicates per group. The five diets contained 1.47%, 1.91%, 2.32%, 2.74%, or 3.15% SID LA. The experimental period lasted 28 d from d 107 of gestation until weaning. Reproductive performance of sows were not affected by dietary SID LA levels (<ce:italic>P</ce:italic> &gt; 0.05); however, piglet weaning weight linearly increased with increasing SID LA levels (<ce:italic>P</ce:italic> = 0.044). A linear-broken relationship between litter weight and SID LA concentration was observed, litter weight on d 14 increased with SID LA until reaching a plateau at 2.36% of SID LA (<ce:italic>P</ce:italic> = 0.005), reaching a plateau at 2.15% of SID LA on d 21 (<ce:italic>P</ce:italic> = 0.083). The content of immunoglobulin A in colostrum (<ce:italic>P</ce:italic> = 0.061) or immunoglobulin M in milk (<ce:italic>P</ce:italic> = 0.088) showed a trend towards a quadratic polynomial relationship with SID LA levels, immunoglobulin M in milk peaked at 2.41% of SID LA (<ce:italic>P</ce:italic> = 0.038). In addition, the tumor necrosis factor-α content in plasma of piglets on d 7 of lactation showed a quadratic polynomial relationship with SID LA levels (<ce:italic>P</ce:italic> = 0.041). The content of immunoglobulin G on d 21 of lactation in sows linearly increased with higher SID LA levels (<ce:italic>P</ce:italic> &lt; 0.001). The level of total antioxidant capacity of piglets on d 21 showed a quadratic polynomial relationship with SID LA levels (<ce:italic>P</ce:italic> = 0.001), which peaked at 2.23 g/kg of SID LA (<ce:italic>P</ce:italic> = 0.002). The glutathione peroxidase activity of piglets on d 7 increased linearly with the increase of SID LA, and reached a plateau at 2.07% of SID LA (<ce:italic>P</ce:italic> = 0.002). In conclusion, using broken line and quadratic regression model, the SID LA requirement for modern lactating sows ranged from 2.07% to 2.43% (133 to 156 g/d) to achieve optimal litter weight, milk immunoglobulin, and antioxidant capacity of sows and piglets.","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"35 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148356672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanically activated zinc oxide enhances growth performance and protects against diarrhea with potentially regulating gut microbiota in weaned piglets","authors":"Zhijie Yuan, Jingqi Huang, Jinxin Guo, Bingbing Zhang, Weicheng Bei, Yong Wang, Yong Li, Hairui Xin, Weigang Xing, Yakuan Huang, Lvhui Sun, Zhangchao Deng","doi":"10.1016/j.aninu.2026.03.002","DOIUrl":"https://doi.org/10.1016/j.aninu.2026.03.002","url":null,"abstract":"High pharmacological doses of zinc oxide (ZnO) are widely used to control post-weaning diarrhea, but environmental pollution and potential adverse effects necessitate the search for effective low-dose alternatives. This study aimed to investigate the effects of dietary level of mechanically activated zinc oxide (MAXACTIVAT/Zn) on growth performance and diarrhea in weaned piglets. A total of 1152 healthy weaned piglets (6.72 ± 0.63 kg) at 21 d of age were randomly assigned to six treatment groups with six replicates of 32 pigs/pen. Piglets were fed a basal diet (BD), or BD supplemented with 100, 200, 400, 600 mg Zn/kg of MAXACTIVAT/Zn (100 Zn, 200 Zn, 400 Zn, and 600 Zn), or 1600 mg Zn/kg of conventional ZnO (1600 Zn) for 28 d. Compared with the BD group, dietary 400 Zn supplementation significantly increased average daily gain during d 1–14, and decreased the feed/gain ratio during d 1–14 and d 1–28 (<ce:italic>P</ce:italic> &lt; 0.05). Additionally, dietary 400 Zn supplementation consistently decreased the diarrhea rate regardless of the experimental period (<ce:italic>P</ce:italic> = 0.001). Notably, these effects are comparable to those observed with 1600 Zn treatment. Moreover, compared to 1600 Zn group, 400 and/or 600 Zn supplementation significantly reduced interleukin-6 (IL-6) content and diamine oxidase (DAO) activity, as well as significantly increased superoxide dismutase (SOD) activity (<ce:italic>P</ce:italic> &lt; 0.05) in serum at d 28. Conversely, no significant differences were observed in serum malondialdehyde (MDA) and interleukin-1β (IL-1β) concentrations between the 400 and/or 600 Zn groups and the 1600 Zn group throughout the experimental period (<ce:italic>P</ce:italic> &gt; 0.05). Further gut microbiome and serum metabolomic analysis found that the abundances of <ce:italic>Lactobacillus</ce:italic>, <ce:italic>Ligilactobacillus</ce:italic>, and <ce:italic>Roseburia</ce:italic> increased and tryptophan metabolism pathway was enriched by 400 Zn supplementation. Furthermore, the differential metabolites involved in tryptophan metabolism significantly correlated with most of differential genera. In conclusion, dietary supplementation with MAXACTIVAT/Zn at 400 mg Zn/kg could exerted a certain positive effect on the growth performance of weaned piglets, which was comparable to or even superior to that of 1600 mg Zn/kg ZnO, indicating that MAXACTIVAT/Zn could serve as an effective alternative to high-dose ZnO used in weaned piglets.","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"62 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148356724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary nisin on growth performance, meat quality, antioxidant status, and microbial homeostasis in broilers","authors":"Xiao Xiao, Tao Wang, Xin Li, Yushan Li, Zhenglie Dai, Agnieszka Lewińska, Caimei Yang","doi":"10.1016/j.aninu.2026.04.002","DOIUrl":"https://doi.org/10.1016/j.aninu.2026.04.002","url":null,"abstract":"The objective of this research is to explore the potential effects of different doses of nisin supplementation on the growth performance, meat quality, immune function, short chain fatty acid (SCFAs), and gut microbiota of broilers. Thus, 640 yellow-feathered broilers (initial body weight: 32.35 ± 0.13 g) at 1 d of age were randomly distributed into four groups (eight replicate pens per treatment and 20 birds per pen), fed with a basal diet with 0 (CON), 250 (N250), 500 (N500), and 1000 IU (N1000) nisin/g for 56 d. Nisin supplementation showed a quadratic increase in ADG from d 29 to 56 (<ce:italic>P</ce:italic> = 0.040) and d 0 to 56 (<ce:italic>P</ce:italic> = 0.048), and effectively increased the redness (a*) (<ce:italic>P</ce:italic> &lt; 0.001), significantly decreased the hardness (<ce:italic>P</ce:italic> &lt; 0.001) and chewiness (<ce:italic>P</ce:italic> &lt; 0.001) of breast muscles. The contents of serum immunoglobulin Y (IgY) at d 56 was significantly increased in the N500 and N1000 groups compared to the CON group (<ce:italic>P</ce:italic> = 0.013). Nisin significantly decreased the contents of interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) (<ce:italic>P</ce:italic> &lt; 0.05) in serum at d 28 and 56. Furthermore, nisin supplementation significantly increased the levels of acetic acid (<ce:italic>P</ce:italic> &lt; 0.05) at d 28 and 56, and propionic acid (<ce:italic>P</ce:italic> = 0.034) at d 56 to enrich the SCFAs content. More importantly, the α-diversity was increased (<ce:italic>P</ce:italic> &lt; 0.05) and the microbial structure were altered by nisin supplementation at d 28 and 56. Differential analysis and linear discriminant analysis with effect size (LEfSe) plots together showed that <ce:italic>Barnesiella</ce:italic> and <ce:italic>Lactobacillus</ce:italic> were enriched in the CON group, while other species, including <ce:italic>Bacteroides</ce:italic>, <ce:italic>Oscillibacter</ce:italic>, and f_Ruminococcaceae, were predominant in the nisin-supplemented groups. In conclusion, nisin supplementation in the diet showed little impact on growth performance, effectively increased the meat quality of breast muscles, and strengthened the immune and antioxidant functions as indicated by pro-inflammatory cytokines and antioxidant enzymes, increased the contents of acetic and propionic acids, and potentially changed the microbial composition of broilers. These findings suggest that nisin has the potential to be used as a natural dietary supplement for broilers.","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"10 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148356669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of a protein feeding strategy on performance and Lysine utilization efficiency in weaned piglets","authors":"Yao Zhu, Thomas Van De Putte, Chengcheng Li, Sam Millet, Jeroen Degroote","doi":"10.1016/j.aninu.2026.01.016","DOIUrl":"https://doi.org/10.1016/j.aninu.2026.01.016","url":null,"abstract":"Dietary reduction of crude protein (CP) can alleviate post-weaning diarrhea (PWD) in piglets but often limits growth even with crystalline amino acid supplementation. This study evaluated how high- or low-protein diets provided for short (10 d) or long (18 d) duration post-weaning affected growth performance and Lys utilization efficiency. A total of 101 piglets (26 to 28 d old; 7.02 ± 0.09 kg) were fed with a high-protein diet (HP; 188 g/kg CP and 12.4 g/kg standardized ileal digestible [SID] Lys) or a low-protein diet (LP; 162 g/kg CP and 10.6 g/kg SID Lys) for 10 or 18 d before transitioning to a common starter diet (179 g/kg CP and 11.8 g/kg SID Lys). Individual feed intake and individual body weight (BW) were continuously monitored using an electronic feeder and weighing station. Individual fecal consistency was assessed on d 6, 10, 14, 18, and 22. The daily SID Lys intake, theoretical SID Lys used for performance, Lys utilization efficiency, and residual SID Lys were calculated or estimated. The effect of protein feeding strategy was analyzed using a mixed model with treatment as a fixed effect and pen as a random effect. Linear and quadratic regressions assessed the relationship of SID Lys intake per metabolic BW (independent variable) with average daily gain (ADG), feed efficiency, Lys utilization efficiency, and residual SID Lys intake (dependent variables) across distinct post-weaning phases. Short-term LP feeding (LP10) did not impair growth, whereas long-term LP feeding (LP18) reduced ADG (<ce:italic>P</ce:italic> = 0.009) and feed efficiency (<ce:italic>P</ce:italic> = 0.010) during d 0–18 without affecting daily feed intake. Long-term HP feeding (HP18) piglets had higher SID Lys intake than LP18 pigs during d 10–18 (<ce:italic>P</ce:italic> = 0.035) and d 0–18 (<ce:italic>P</ce:italic> = 0.051). Lys utilization efficiency did not differ among treatments within 18 d post-weaning but was lower in HP18 than LP18 during d 18–39 and d 0–39 (<ce:italic>P</ce:italic> = 0.002), consistent with residual SID Lys intake. Lys utilization efficiency was positively associated with SID Lys intake per metabolic BW during d 0–10, but negatively associated thereafter (<ce:italic>P</ce:italic> &lt; 0.001). Overall, prolonged feeding of a LP diet compromised growth but enhanced Lys utilization efficiency post-weaning.","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"47 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148312997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromatin accessibility of the jejunum in two high-yielding laying hen strains under divergent mineral phosphorus supply during the transition to egg laying","authors":"Yosef A. Abitew, Henry Reyer, Frieder Hadlich, Michael Oster, Nares Trakooljul, Vera Sommerfeld, Markus Rodehutscord, Klaus Wimmers, Siriluck Ponsuksili","doi":"10.1016/j.aninu.2026.01.015","DOIUrl":"https://doi.org/10.1016/j.aninu.2026.01.015","url":null,"abstract":"Optimizing dietary phosphorus (P) levels is crucial for sustainable egg production, hen welfare, productivity, and reducing excessive P excretion. Dietary P influences systemic physiological responses, including gene expression dynamics and epigenetic regulation, particularly in the gut. Epigenetic modifications, including chromatin accessibility, play a crucial role in gene regulation by integrating genetic and environmental signals. It is hypothesized that age, strain, and mineral P levels together influence the epigenetic and transcriptional landscape in the jejunum during the transition to egg-laying. Using the domestic chicken (&lt;ce:italic&gt;Gallus gallus domesticus&lt;/ce:italic&gt;), two commercial laying-hen strains, Lohmann Brown (LB) and Lohmann Selected Leghorn (LSL) were studied. The experiment followed a 2 × 2 × 2 factorial design including two strains (LB vs. LSL), two production periods (transition before the onset of egg laying vs. onset of laying), and two dietary P levels (without supplemental mineral P [P−] or with 1 g/kg supplemental P from monocalcium phosphate [P+]). A total of 80 hens were used, 10 per strain × period × diet combination, in a randomised complete block design. In each period, hens were housed individually in metabolic units for 4 weeks, beginning either before the onset of egg-laying (transition; placed at 15 weeks of age, sampled at week 19) or after the onset of egg-laying (onset of laying; placed at 20 weeks of age, sampled at week 24). At placement, mean initial body weight was 1228 ± 134 g (LB) and 1036 ± 70 g (LSL) in the transition period, and 1552 ± 93 g (LB) and 1244 ± 64 g (LSL) in the onset-of-laying period. Chromatin accessibility was assessed using an assay for transposase-accessible chromatin with sequencing (ATAC-Seq). The analysis revealed distinct strain-specific chromatin accessibility profiles, identifying 6610 differentially accessible regions (DAR) (adjusted &lt;ce:italic&gt;P&lt;/ce:italic&gt; &lt; 0.01). The LSL strain genome exhibited more regions with reduced accessibility (3603 DAR) compared to the LB strain (adjusted &lt;ce:italic&gt;P&lt;/ce:italic&gt; &lt; 0.01). Extensive chromatin remodelling was observed during the transition to laying onset (7884 DARs), with greater loss of accessibility at 24 weeks (4193 DARs). Dietary P had subtler effects (1671 DARs; &lt;ce:italic&gt;P&lt;/ce:italic&gt; &lt; 0.01), including increased accessibility at loci such as &lt;ce:italic&gt;CALB1&lt;/ce:italic&gt; under the P- diet. Enrichment analysis of transcripts nearest these DARs revealed pathways related to lipid transport, fatty acid metabolism, Wnt signalling, transforming growth factor-β (TGF-β) signalling, and calcium signalling. Additionally, ANalysis Algorithm for Networks Specified by Enhancers (ANANSE) integrates predicted transcription factor (TF) binding in accessible chromatin with RNA-Seq expression to weight gene regulatory networks (GRN) edges and rank TF influence (P+ vs. P-), highlighting regulators linked to bone/cartilage remodell","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"69 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148288370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation of lysolecithin improves growth performance of piglets fed a low-nutrient density diet by modulating nutrient utilization and metabolism","authors":"Huaqi Song, Zhiming Zhang, Renjie Yao, Qiang Zhou, Yang Liu, Jin Jin, Junqi Jin, Zheng Luo, Jiayong Tang, Bin Feng, Shengyu Xu, Yan Lin, Lun Hua, Yong Zhuo, De Wu, Lianqiang Che","doi":"10.1016/j.aninu.2026.01.011","DOIUrl":"https://doi.org/10.1016/j.aninu.2026.01.011","url":null,"abstract":"This study aimed to investigate the effects of dietary lysolecithin (LPI) supplementation on growth performance, nutrient utilization, metabolism, and muscle development in piglets fed a low-nutrient density (LN) diet. A total of 96 nursery piglets (17.0 ± 0.2 kg in initial body weight [BW] and 50 d of age) were randomly assigned to three groups in a randomized complete block design and received either the normal-nutrient diet (CON), a LN diet, or the LN diet supplemented with 400 mg/kg lysolecithin (LN-LPI) for 28 d. Each dietary treatment included 8 replicates of 4 piglets each. Results showed that the LN-LPI diet improved the average daily gain (ADG; <ce:italic>P</ce:italic> = 0.040) during the first two weeks, feed-to-gain ratio (F:G; <ce:italic>P</ce:italic> = 0.032), and BW (<ce:italic>P</ce:italic> = 0.065) throughout the experimental period, reaching levels comparable to those of piglets in the CON group. Compared with the LN diet, the growth-promoting effect of LPI may be attributed to its role in increasing the digestibility of ether extract (EE; <ce:italic>P</ce:italic> &lt; 0.001) and gross energy (GE; <ce:italic>P</ce:italic> = 0.022) in diets, as well as increasing the relative weight of psoas major muscle (PMM) and the EE (<ce:italic>P</ce:italic> = 0.049) and crude protein (CP) content (<ce:italic>P</ce:italic> = 0.007) in muscle. Accordingly, compared with the LN diet, LPI supplementation decreased the blood urea nitrogen (<ce:italic>P</ce:italic> = 0.003) and total cholesterol (TC) concentrations (<ce:italic>P</ce:italic> = 0.017), and increased total superoxide dismutase (T-SOD) levels in plasma (<ce:italic>P</ce:italic> = 0.005) and liver (<ce:italic>P</ce:italic> = 0.006), along with the lower malondialdehyde (MDA) levels in blood (<ce:italic>P</ce:italic> &lt; 0.001) and liver (<ce:italic>P</ce:italic> = 0.003). In addition, microarray analysis indicated that dietary LPI supplementation promoted expression of lipid synthesis and transport-related genes in the liver (<ce:italic>FAS</ce:italic>, <ce:italic>P</ce:italic> = 0.040; <ce:italic>FABP5</ce:italic>, <ce:italic>P</ce:italic> = 0.001; and <ce:italic>SLC27A6</ce:italic>, <ce:italic>P</ce:italic> = 0.025), and amino acid (AA) transport and synthesis-related genes (<ce:italic>LAT</ce:italic>, <ce:italic>P</ce:italic> = 0.021; <ce:italic>LAMC3</ce:italic>, <ce:italic>P</ce:italic> = 0.004), compared with the LN diet in muscle. In conclusion, this study demonstrated that the LN-LPI diet mitigated the compromised growth performance commonly observed under nutrient restriction, potentially mediated by improving nutrient utilization and redox status, and modulating lipid and protein metabolism.","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"17 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148289932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}