Animal Nutrition最新文献

{"title":"Dietary coated sodium selenite enhances muscle growth associated with mitochondrial function in sub-adult grass carp (Ctenopharyngodon idellus)","authors":"Fei Zhao, Pei Wu, Weidan Jiang, Hongyun Zhang, Yang Liu, Yaobin Ma, Hongmei Ren, Xiaowan Jin, Yinggu Kuang, Lin Feng, Xiaoqiu Zhou","doi":"10.1016/j.aninu.2025.12.011","DOIUrl":"https://doi.org/10.1016/j.aninu.2025.12.011","url":null,"abstract":"","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"63 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147620165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guanidinoacetic acid improves flesh quality associated with collagen synthesis and nucleotide metabolism in gibel carp CAS V (Carassius gibelio, CAS V)","authors":"Yu Wang, Yan Zhang, Liyun Wu, Chaoyue Li, Qiaozhen Chen, Dong Han, Haokun Liu, Zhimin Zhang, Shouqi Xie, Junyan Jin","doi":"10.1016/j.aninu.2026.01.003","DOIUrl":"https://doi.org/10.1016/j.aninu.2026.01.003","url":null,"abstract":"","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"118 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147598216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of the iron requirements and its determination in commercial poultry.","authors":"Natalie K Morgan, Judith L O'Keeffe, Mark J Hackett","doi":"10.1016/j.aninu.2026.01.002","DOIUrl":"10.1016/j.aninu.2026.01.002","url":null,"abstract":"<p><p>Iron (Fe) is an essential mineral for poultry, playing a critical role in oxygen transport and energy metabolism. Consequently, it is routinely supplemented into commercial poultry diets to safeguard against deficiencies that can negatively impact productive performance. Conversely, excessive dietary Fe can compromise performance by inducing oxidative stress, reducing absorption of other nutrients and negatively disrupting the gastrointestinal microbiota. The bioavailability, and thus absorption, of Fe is dictated by the form it is present in the feed, with haem, the organic form of Fe, being preferentially absorbed. Modern poultry diets, depending on location or context, may be devoid of animal proteins, and therefore without haem. Inorganic Fe sulphates have been the primary source of supplemental Fe in poultry diets, via premixes. These sources may provide Fe in the ferric state (Fe³⁺), which must first be converted to a ferrous state (Fe²⁺) for uptake, though it is more commonly supplied as ferrous sulphate monohydrate (Fe²⁺). The poor bioavailability of inorganic Fe has led to increased interest in alternative organic Fe sources, such Fe-amino acid chelates and Fe proteinates, with higher absorption efficiency at lower active inclusion levels. Iron absorption is also dictated by interactions with anti-nutrients, notably phytic acid and mineral antagonists. There is considerable variation in recommended supplemental Fe levels for poultry across published guidelines, feeding standards and scientific articles. For example, in broilers and broiler breeders recommended total Fe ranges from 20 to 110 mg/kg and in laying hens from 30 to 80 mg/kg. Advances in feed formulation, including use of organic sources of Fe, reduced concentration of antagonistic trace minerals and phytase supplementation, has enhanced bioavailability of Fe, indicating that commercial poultry may now be over-supplemented with Fe. Updated and context-specific Fe recommendations are essential for optimising poultry productivity. This review examines how to assess Fe utilisation in poultry, including methods for determining Fe status, proposes Fe recommendations for broilers, broiler breeders and laying hens based on outputs from research trials, and explores how Fe interacts with other nutrients and the microbiota.</p>","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"26 ","pages":"1-21"},"PeriodicalIF":8.3,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative effects of bile salts and free bile acids on intestinal health of grouper (Epinephelus spp.) fed a high cottonseed protein concentrate diet","authors":"Fan Wang, Yuchen Bao, Yuhua Zhao, Junxiang Lai, Ermeng Yu, Fajun Jiang, Jia Xu","doi":"10.1016/j.aninu.2025.12.008","DOIUrl":"https://doi.org/10.1016/j.aninu.2025.12.008","url":null,"abstract":"","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"59 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147496615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-Acetylcysteine alleviates glycinin-induced intestinal damage in common carp: Multi-target regulation inhibiting apoptosis and restoring mucosal barrier integrity","authors":"Rui Zhu, Deng-Lai Li, Bao-Yuan Zhang, Feng-Feng Zhang, Ke Zhou, Gui-Xin Qin, Li-Fang Wu, Feng-Xing Xie","doi":"10.1016/j.aninu.2026.02.001","DOIUrl":"https://doi.org/10.1016/j.aninu.2026.02.001","url":null,"abstract":"Glycinin is a major anti-nutritional factor in soybeans and can induce growth inhibition and intestinal damage in aquatic animals. N-Acetylcysteine (NAC) possesses antioxidant and anti-inflammatory properties while promoting intestinal mucosal development. Therefore, this study aimed to elucidate the protective effects and underlying mechanisms of NAC against glycinin-induced intestinal damage in common carp (<ce:italic>Cyprinus carpio</ce:italic>). A total of 450 juvenile common carp (2.93 ± 0.03 g) were randomly assigned to five groups: a control group (CK), an 8.00% glycinin-damaged group (Gly), and three NAC-treated groups receiving 0.15%, 0.30% and 0.60% NAC supplementation (Gly-N1, Gly-N2, and Gly-N3). Each group contained three replicates for a 56-d feeding trial. Results showed that dietary 0.30%–0.60% NAC supplementation effectively alleviated the glycinin-induced impairments in growth performance and feed utilization, while concurrently restoring intestinal protease activity and muscle protein deposition (<ce:italic>P</ce:italic> &lt; 0.05). NAC mitigated the intestinal morphological damage induced by glycinin, including mucosal fold atrophy and microvilli shedding, and enhanced barrier integrity by upregulating the mRNA expression of tight junction proteins occludin, claudin-3, claudin-7 and <ce:italic>zo-1</ce:italic>, and reducing intestinal permeability (<ce:italic>P</ce:italic> &lt; 0.05). Intestinal transcriptome analysis indicated NAC's ameliorative effects involving inflammation- and apoptosis-related pathways. Mechanistically, NAC exerted multi-target protection: it inhibited the MAPK/PI3K-AKT/NF-κB inflammatory network by downregulating the phosphorylation of p38, JNK, PI3K and AKT, and the expression of NF-κB p65, thereby inhibiting pro-inflammatory cytokines IL-1β and TNF-α release (<ce:italic>P</ce:italic> &lt; 0.05). Concurrently, NAC activated the Nrf2 antioxidant pathway to counteract oxidative stress. Furthermore, NAC inhibited the mitochondrial apoptosis pathway by modulating Bcl-2/Bax expression and inhibiting <ce:italic>Caspases</ce:italic> activation, while restoring ATPase activity and membrane potential to improve intestinal mitochondrial function. In summary, dietary supplementation with 0.30%–0.60% NAC alleviated glycinin-induced intestinal damage through a multifaceted mechanism involving inhibition of inflammatory signaling, activation of antioxidant defense, and inhibition of mitochondrial apoptosis.","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"15 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147524929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating dietary cholesterol and taurine interactions: Implications for growth performance, bile acid metabolism, and lipid regulation in Chinese mitten crab (Eriocheir sinensis)","authors":"Qiuran Yu, Xiaodan Wang, Song Wang, Han Wang, Chuanjie Qin, Jianguang Qin, Erchao Li, Liqiao Chen","doi":"10.1016/j.aninu.2025.09.019","DOIUrl":"https://doi.org/10.1016/j.aninu.2025.09.019","url":null,"abstract":"Cholesterol serves as a precursor for bile acids and molting hormones, while taurine is involved in bile acid conjugation and lipid regulation in vertebrates. However, their synergetic roles in crustacean nutrition remain poorly understood. This study investigated the interactions between dietary cholesterol (0%, 0.64%, and 1.00%) and taurine (0% and 0.65%) in juvenile Chinese mitten crabs (<ce:italic>Eriocheir sinensis</ce:italic>). A total of 960 juvenile crabs (3.08 ± 0.02 g) were randomly assigned to six dietary treatments (four replicates per treatment, 40 crabs per replicate) in a 3 × 2 factorial design for 8 weeks. Growth performance, body proximate composition, serum and hepatopancreas biochemical indices, bile acid profiles, and expression of genes related to lipid metabolism and the nutrient-sensitive mammalian target of rapamycin (mTOR) signaling pathway were evaluated. Significant improvements in final body weight, weight gain, specific growth rate, and feed conversion ratio were observed in crabs fed the diet containing 0.64% cholesterol and 0.65% taurine (<ce:italic>P</ce:italic> &lt; 0.05). Dietary cholesterol significantly increased hepatopancreas total cholesterol and bile acid contents (<ce:italic>P</ce:italic> &lt; 0.05), particularly taurohyodeoxycholic acid and epi-allolithocholic acid, while taurine supplementation further elevated cholic acid content. Taurine effectively reduced serum total cholesterol and low-density lipoprotein cholesterol concentrations (<ce:italic>P</ce:italic> &lt; 0.05), especially at higher dietary cholesterol levels. The combination of cholesterol and taurine significantly alleviated antioxidant impairment by increasing superoxide dismutase activity (<ce:italic>P</ce:italic> &lt; 0.001), and upregulated key genes in lipid metabolism (<ce:italic>fas</ce:italic>, <ce:italic>cpt1a</ce:italic>), cholesterol transport (<ce:italic>abcg8</ce:italic>), and mTOR pathway components (<ce:italic>mtorc1</ce:italic>, <ce:italic>s6k1</ce:italic>, <ce:italic>4ebp1</ce:italic>) (<ce:italic>P</ce:italic> &lt; 0.05). Dietary 0.64% cholesterol in combination with 0.65% taurine optimized growth performance and improved the health of <ce:italic>E. sinensis</ce:italic>. Taurine enhanced cholesterol utilization by facilitating its conversion into bile acids and activating key nutrient-sensing pathways such as the mTOR pathway, thereby improving lipid metabolism and antioxidant capacity. These findings reveal a synergistic metabolic interaction in which taurine enhances the nutritional value of cholesterol by facilitating its conversion into signaling-active bile acids and directly modulating lipid metabolism pathways.","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"52 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147524930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory effects of soy isoflavones on ovarian development in female Chinese mitten crabs (Eriocheir sinensis)","authors":"Long He, Jinping Li, Dexiang Cao, Zhijun Liu, Chuanjie Qin, Xiaodan Wang, Jianguang Qin, Erchao Li, Liqiao Chen","doi":"10.1016/j.aninu.2025.10.014","DOIUrl":"https://doi.org/10.1016/j.aninu.2025.10.014","url":null,"abstract":"Soy isoflavones are phytoestrogens that exhibit both estrogenic and/or antiestrogenic effects. This research investigated the potential of soy isoflavones as functional feed additives to promote ovarian development in female Chinese mitten crabs (<ce:italic>Eriocheir sinensis</ce:italic>). One hundred ninety-two crabs (101.52 ± 4.57 g) were randomly assigned to four groups (six replicates per group and eight crabs per replicate), and fed diets supplemented with 0, 32.51, 70.83, or 369.03 mg/kg soy isoflavones for 11 weeks. Compared to the control group, supplementation with 32.51 mg/kg soy isoflavones significantly increased the gonadosomatic index, hemolymph vitellogenin content, and the <ce:italic>vtg</ce:italic> mRNA levels in the hepatopancreas and ovary (<ce:italic>P</ce:italic> &lt; 0.05). Moreover, supplementation with 32.51 and 70.83 mg/kg soy isoflavones significantly promoted yolk granule formation (<ce:italic>P</ce:italic> &lt; 0.05). At the molecular level, soy isoflavones modulated estradiol levels and activated the estrogen-related receptor signaling. They also upregulated the expression levels of <ce:italic>esrrb</ce:italic>, <ce:italic>hsd3b2</ce:italic> and <ce:italic>hsd17b6</ce:italic> genes (<ce:italic>P</ce:italic> &lt; 0.05). Additionally, they increased estradiol synthesis through activating the cyclic adenosine monophosphate/PKA/CREB protein signaling pathway. High dose supplementation (369.03 mg/kg) did not significantly affect ovarian development (<ce:italic>P</ce:italic> &gt; 0.05). Therefore, soy isoflavones exhibit a U-shaped effect on ovarian development of <ce:italic>E. sinensis</ce:italic>, with 32.51 mg/kg being an effective dose for promoting ovarian maturation.","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"33 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147524931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional alternatives to commercial lipid sources: Impact of the dietary inclusion of black soldier fly (Hermetia illucens) larvae oil on broiler chicken productivity, breast meat quality traits and caeca microbiome","authors":"Jonathan Dayan, Alessandra De Cesare, Francesca Soglia, Marco Zampiga, Valentina Indio, Emilia Luigia Antenucci, Massimiliano Petracci, Federico Sirri","doi":"10.1016/j.aninu.2025.12.005","DOIUrl":"https://doi.org/10.1016/j.aninu.2025.12.005","url":null,"abstract":"","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"16 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147447657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of fraction size on the efficacy of xylo-oligosaccharides in broiler chicken diets","authors":"Andrew Wallace, Michael.R. Bedford, Gemma González-Ortiz, Natalie K. Morgan","doi":"10.1016/j.aninu.2025.12.007","DOIUrl":"https://doi.org/10.1016/j.aninu.2025.12.007","url":null,"abstract":"","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"93 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147447656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The response of broilers to xylanase and β-glucanase combination in maize-based diets containing wheat distillers’ dried grain with solubles","authors":"Eunjoo Kim, Mingan Choct, Anna Fickler, Leon Hall, Tamsyn M. Crowley, Nishchal K. Sharma","doi":"10.1016/j.aninu.2025.12.006","DOIUrl":"https://doi.org/10.1016/j.aninu.2025.12.006","url":null,"abstract":"Broiler diets containing wheat distillers’ dried grain with solubles (wDDGS) have higher levels of non-starch polysaccharides (NSP) compared to conventional maize-soybean meal-based diets, which can increase intestinal viscosity and impair nutrient utilisation. This study investigated the effects of supplemental xylanase and β-glucanase (XG) preparation on growth performance, ileal flow of soluble NSP constituent sugars, and the gastrointestinal environment in broilers fed maize-based diets with varying wDDGS levels. A total of 768 mixed-sex Cobb 500 broilers (on hatch day and 40 ± 1 g) were assigned to 6 treatments in a 3 × 2 factorial arrangement, with wDDGS levels (none), 6%–12% (medium), 12%–20% (high) with or without XG supplementation across eight replicate pens of 16 birds each, totaling 128 birds per treatment. From d 0–10, XG and wDDGS interacted for weight gain (WG; &lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.019), with XG improving WG only in broilers fed high wDDGS diets but not in others. High wDDGS worsened feed conversion ratio (FCR; &lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.014), while XG tended to improve it (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.079). From d 21–35, XG increased WG (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.001) and improved FCR (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.006) regardless of wDDGS level. From d 0–35, XG increased WG (&lt;ce:italic&gt;P&lt;/ce:italic&gt; &lt; 0.001) and tended to improve FCR regardless of wDDGS level (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.097). Medium and high wDDGS diets raised ileal viscosity at d 21 (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.020) and high wDDGS at d 35 (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.002). XG lowered ileal viscosity at d 21 (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.006) and d 35 (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.001). Medium and high wDDGS diets resulted in higher ileal flow of soluble NSP constituent sugars, including soluble arabinose, mannose, and glucose (&lt;ce:italic&gt;P&lt;/ce:italic&gt; &lt; 0.001) vs. the control. XG reduced soluble arabinose (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.012) and total soluble NSP constituent sugars (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.007). Dietary XG and wDDGS interacted for ileal soluble xylose concentration within the NSP fraction (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.018), where XG reduced soluble xylose in broilers fed medium and high wDDGS diets but not the control (no wDDGS). High wDDGS diets increased the ileal flow of insoluble NSP constituent sugars (&lt;ce:italic&gt;P&lt;/ce:italic&gt; &lt; 0.001) vs. the control diet with no wDDGS. XG supplementation did not affect ileal insoluble NSP constituent sugars (&lt;ce:italic&gt;P&lt;/ce:italic&gt; &gt; 0.05), but tended to elevate xylose in the free oligosaccharide fraction in the ileum (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.053). XG increased caecal propionic acid (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.004) and tended to increase caecal short-chain fatty acids (&lt;ce:italic&gt;P&lt;/ce:italic&gt; = 0.060). In conclusion, XG supplementation enhanced broiler performance in maize-based diets with wDDGS by reducing soluble NSP and small intestinal viscosity while promoting ","PeriodicalId":8184,"journal":{"name":"Animal Nutrition","volume":"109 1","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147393540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}