Animal Reproduction最新文献

{"title":"Is FSH combined with equine chorionic gonadotropin able to modify lipid metabolism in bovine superstimulated antral follicles?","authors":"Priscila Helena Santos, Fernanda Fagali Franchi, Sarah Gomes Nunes, Patricia Kubo Fontes, Alan Brunholi Giroto, Fernanda Mani, Anthony César de Souza Castilho","doi":"10.1590/1984-3143-AR2023-0063","DOIUrl":"10.1590/1984-3143-AR2023-0063","url":null,"abstract":"<p><p>Lipid metabolism is essential for ensuring oocyte maturation and embryo development. β-Oxidized fatty acids (FA) are a potent source of energy for cells, particularly for bovine somatic follicular cells. Superstimulatory protocols using follicle stimulating hormone (FSH) or FSH combined with equine chorionic gonadotropin (eCG) are capable of stimulating the follicular microenvironment and drive the expression of biomarker genes associated with lipid metabolism in the cumulus-oocyte complex (COC) for better embryo development. In this study, we assesed the effects of FSH and FSH/eCG protocols on the expression of genes related to lipid metabolism in bovine granulosa cells (GCs). Further, we measured triglyceride levels in follicular fluid (FF) obtained from both superstimulatd and non-superstimulated cows (synchronized cows). In summary, superstimulation with gonadotropins maintained the TG levels in bovine FF and ensured GCs mRNA abundance of <i>ACSL1, ACSL3, ACSL6, SCD, ELOVL5, ELOVL6, FASN, FADS2,</i> and <i>SREBP1.</i> We, however, found the abundance of <i>CPTIB</i> mRNA to be lower in GCs obtained from cows subjected to FSH/eCG protocols than synchronized cows. In conclusion, the findings of this study showed that ovarian superstimulation around the preovulatory phase has a mild impact on the lipid metabolism in GCs.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230063"},"PeriodicalIF":1.6,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory and anti-oxidant action of tadalafil in testicular regeneration process after heat stress.","authors":"Fernando Leonel da Silva, Fernanda Carolina Ribeiro Dias, Sandra Maria Torres, Virginia Maria Barros de Lorena, Sebastião Rogerio de Freitas Silva, Vinicius Vasconcelos Gomes de Oliveira, Emanoel Felipe de Oliveira, Pierre Castro Soares, Valdemiro Amaro da Silva","doi":"10.1590/1984-3143-AR2023-0095","DOIUrl":"10.1590/1984-3143-AR2023-0095","url":null,"abstract":"<p><p>Tadalafil, a potent phosphodiesterase inhibitor 5 (PDE-5), is commonly used for the management of erectile dysfunction. However, its therapeutic potential extends beyond this indication. This study aimed to investigate the impact of tadalafil on the recovery of testicular parenchyma in male Wistar rats exposed to testicular thermal stress. Fifty-four Wistar rats were subjected to testicular thermal stress and randomly assigned to receive either tadalafil treatment (TAD) or no treatment (control). TAD was administered intraperitoneally at a dose of either 0.9 mg/kg or 1.8 mg/kg. Biometric parameters, histopathological assessment of the testis, serum testosterone levels, oxidative stress, and interleukin levels were evaluated on days 7, 15, and 30 after thermal shock. The animals were euthanized at the end of each experimental period, and samples were collected. TAD treatment maintained testicular weight and reduced the testicular degenerative process up to day 7 post-injury. However, despite TAD therapy, serum testosterone levels were decreased in the treated groups at days 7 and 15 post-thermal stress. TAD also decreased TNF-α and NO levels at different doses but had no effect on IL-6. The treatment with TAD after heat shock demonstrated anti-inflammatory and antioxidant properties but did not prevent the aggravation of testicular lesions in subsequent periods, even with the systematic reduction in TNF-α and NO levels. Therefore, this selective PDE-5 inhibitor, at the dosages used, did not have a positive impact on testosterone levels during the post-thermal stress period, which could compromise the resumption of the spermatogenic process.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230095"},"PeriodicalIF":1.6,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA sequencing and gene co-expression network of <i>in vitro</i> matured oocytes and blastocysts of buffalo.","authors":"Priscila Di Paula Bessa Santana, Kenny da Costa Pinheiro, Lino César de Souza Pereira, Soraya Silva Andrade, Flávia Figueira Aburjaile, Priscilla do Carmo de Azevedo Ramos, Eduardo Baia de Souza, Nathalia Nogueira da Costa, Marcela da Silva Cordeiro, Simone do Socorro Damasceno Santos, Moysés Dos Santos Miranda, Rommel Thiago Jucá Ramos, Artur Luiz da Costa da Silva","doi":"10.1590/1984-3143-AR2023-0131","DOIUrl":"10.1590/1984-3143-AR2023-0131","url":null,"abstract":"<p><p>In reproductive technologies, uncovering the molecular aspects of oocyte and embryo competence under different conditions is crucial for refining protocols and enhancing efficiency. RNA-seq generates high-throughput data and provides transcriptomes that can undergo additional computational analyses. This study presented the transcriptomic profiles of <i>in vitro</i> matured oocytes and blastocysts produced <i>in vitro</i> from buffalo crossbred (<i>Bubalus bubalis</i>), coupled with gene co-expression and module preservation analysis. Cumulus Oophorus Complexes, obtained from slaughterhouse-derived ovaries, were subjected to <i>in vitro</i> maturation to yield metaphase II oocytes (616) or followed <i>in vitro</i> fertilization and culture to yield blastocysts for sequencing (526). Oocyte maturation (72%, ±3.34 sd) and embryo development (21.3%, ±4.18 sd) rates were obtained from three <i>in vitro</i> embryo production routines following standard protocols. Sequencing of 410 metaphase II oocytes and 70 hatched blastocysts (grade 1 and 2) identified a total of 13,976 genes, with 62% being ubiquitously expressed (8,649). Among them, the differentially expressed genes (4,153) and the strongly variable genes with the higher expression (fold-change above 11) were highlighted in oocytes (<i>BMP15</i>, <i>UCHL1</i>, <i>WEE1</i>, <i>NLRPs, KPNA7</i>, <i>ZP2,</i> and <i>ZP4</i>) and blastocysts (<i>APOA1</i>, <i>KRT18</i>, <i>ANXA2</i>, <i>S100A14</i>, <i>SLC34A2</i>, <i>PRSS8</i> and <i>ANXA2</i>) as representative indicators of molecular quality. Additionally, genes exclusively found in oocytes (224) and blastocysts (2,200) with specific biological functions were identified. Gene co-expression network and module preservation analysis revealed strong preservation of functional modules related to exosome components, steroid metabolism, cell proliferation, and morphogenesis. However, cell cycle and amino acid transport modules exhibited weak preservation, which may reflect differences in embryo development kinetics and the activation of cell signaling pathways between buffalo and bovine. This comprehensive transcriptomic profile serves as a valuable resource for assessing the molecular quality of buffalo oocytes and embryos in future <i>in vitro</i> embryo production assays.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230131"},"PeriodicalIF":1.6,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11192227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic review of hormonal strategies to improve fertility in rams.","authors":"Estela Garza-Brenner, Fernando Sánchez-Dávila, Keyla Mauleón-Tolentino, Cecilia Carmela Zapata-Campos, Carlos Luna-Palomera, Javier Hernandez-Melendez, Marisol Gonzalez-Delgado, José Fernando Vázquez-Armijo","doi":"10.1590/1984-3143-AR2024-0007","DOIUrl":"10.1590/1984-3143-AR2024-0007","url":null,"abstract":"<p><p>Reviewing the current state of knowledge on reproductive performance and productive traits in rams has many advantages. First, the compilation of this information will serve as a literature resource for scientists conducting research around the world and will contribute to the understanding of the data collected and interpreted by researchers on the different hormonal strategies used to improve reproductive performance in rams. Second, it will allow scientists to identify current knowledge gaps and set future research priorities in ram reproduction. Rams play an important role in the global flock economy, but their reproductive analysis has been limited in the use of hormonal technologies to increase the productivity of sheep flocks. In this review, we cite the most important works on six hormones that, in one way or another, modify the hypothalamus-pituitary-gonadal axis, at different doses, in and out of the reproductive season, breeds, application methods, among other factors. The overall aim is to increase the reproductive efficiency of rams in different scenarios and, in some cases, of other species due to the lack of limited information on rams.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20240007"},"PeriodicalIF":1.6,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11189135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth performance, reproductive status, and chromosomal instability in triploid Nile tilapias.","authors":"Williane Ferreira Menezes, Érika Ramos Alvarenga, Rafael Henrique Nóbrega, Luiz Renato França, Marcelo Rezende Luz, Ludson Guimarães Manduca, Franklin Fernando Batista da Costa, Vinícius Monteiro Bezerra, Arthur Francisco de Araújo Fernandes, Eduardo Maldonado Turra","doi":"10.1590/1984-3143-AR2023-0147","DOIUrl":"10.1590/1984-3143-AR2023-0147","url":null,"abstract":"<p><p>Reproductive control is one of the biggest challenges in tilapia production and triploidy was developed as an alternative to sterilization. In general, polyploids present chromosomal instability but for triploid Nile tilapia it has yet to be reported. This study evaluated the chromosomal instability from juveniles to adulthood, growth performance and gonadal status of tilapia hatched from eggs submitted or not to heat shock for triploid induction. Nile tilapia oocytes were fertilized (1,476 oocytes), half of the eggs were subjected to a four-minute shock in 41 °C water four minutes after fertilization and the other half were not (Control group). The eggs were incubated (at 27°C) and 160 larvae from the treated group hatched and survived after yolk sac absorption. The determination of ploidy was performed by flow cytometry at 85^th (juveniles) and 301^st (adults) days of age post yolk sac absorption. At the time of the first cytometry analysis there were 73 surviving juveniles from the treated group, and only 14 were confirmed triploid. However, at the analysis of adult ploidy, one out of 8 surviving adult tilapias from the 14 confirmed triploid juveniles remained triploid. Gonadal histology showed that the non-remaining triploids continued to produce gametes. The growth performance of triploid tilapia was initially superior to that of diploid tilapia during the juvenile phase, but similar in adults. Once the chromosome sets are lost and the tilapias become diploid again, at least in tissues with a high proliferation rate, such as the hematopoietic tissue that was analyzed (and possibly in gonads), all possible advantages of triploids are probably lost. Thus, our results suggest that, due to genomic instabilities, the triploid generation of tilapia has low efficiency.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230147"},"PeriodicalIF":1.7,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11129864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141157851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trans 10, cis 12-conjugated linoleic acid reduced reproductive ability by disrupting the estrus cycle in female mice.","authors":"Shuai Yu, Baozhu Wang, Yu Rao, Mei Liu, Luwen Liang, Kemian Gou","doi":"10.1590/1984-3143-AR2024-0010","DOIUrl":"10.1590/1984-3143-AR2024-0010","url":null,"abstract":"<p><p>As a positional and geometrical isomer of linoleic acid, trans 10, cis 12 conjugated linoleic acid (t10c12-CLA) reduces white fat by reducing food intake, modulating lipid metabolism, and stimulating energy expenditure. However, the t10c12-CLA products are mostly mixtures, making it difficult to obtain accurate results. Studies are needed to investigate the effects of pure t10c12-CLA on animals and humans. In this study, we used the biallelic transgenic (tg) mice, which could produce t10c12-CLA itself, to investigate the effects of pure t10c12-CLA on female reproductive ability. The results showed that the body and relative ovary weights had no significant difference between tg and wild-type (wt) littermates at ages 3 or 10 weeks. While the fecundity test found that tg mice had a significantly longer first litter time (32.0 ± 4.70 days vs. 21.3 ± 2.31 days, <i>P</i><0.05), and a significantly lower number of litters (4.75 ± 2.75 vs. 6.67 ± 0.57, <i>P</i><0.05) when compared with wt mice during continuous mating within seven months. Hormone profiles showed that serum estradiol levels did not change in tg mice; however, significantly (<i>P</i><0.05) decreased progesterone and increased prostaglandin E2 levels were observed in tg mice compared with those of wt mice. Hematoxylin-eosin staining showed no pathological characteristics in tg ovaries, except for the increased atresia follicles (<i>P</i><0.05). Moreover, the tg mice had a significantly more extended diestrus period than the wt mice (48.4 ± 6.38% vs. 39.6 ± 3.81%, <i>P</i><0.05). In summary, t10c12-CLA could affect serum progesterone and prostaglandin E2 levels, lead to a disordered estrus cycle, and impact the reproductive performance of female mice. This study provided theoretical and biosafety recommendations for applying t10c12-CLA in female mammals.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20240010"},"PeriodicalIF":1.6,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11095849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm HSP70: may not be an age-dependent gene but is associated with field fertility in Bali bulls (<i>Bos sondaicus</i>).","authors":"Dian Tria Fatmila, Berlin Pandapotan Pardede, Tulus Maulana, Syahruddin Said, Yudi Yudi, Bambang Purwantara","doi":"10.1590/1984-3143-AR2023-0048","DOIUrl":"https://doi.org/10.1590/1984-3143-AR2023-0048","url":null,"abstract":"<p><p>This study aimed to analyze the characteristics of the HSP70 gene and protein in spermatozoa of Bali bulls of different age groups and to examine its potential as a biomarker determining bull fertility. This study used frozen semen produced from six Bali bulls divided into two groups based on age (≤ 9 years and ≥ 12 years). Parameters of frozen semen quality analyzed included sperm motility and kinetics using computer-assisted semen analysis, sperm morphological defects using Diff-Quick staining, acrosome integrity using FITC-PNA staining, and DNA fragmentation using acridine orange staining. HSP70 gene expression characterization was analyzed using qRT-PCR, and HSP70 protein abundance was analyzed using enzyme immunoassays. Fertility field data were obtained by analyzing the percentage conception rate for each bull based on the artificial insemination service data contained in the Indonesian-integrated system of the National Animal Health Information System (iSIKHNAS). The results showed significant differences (P<0.05) in total and progressive motility, morphological defects of the neck and midpiece, and tail of sperm, and acrosome integrity between the age groups of Bali bulls. HSP70 gene expression and protein abundance showed no significant differences (P>0.05) in different age groups. HSP70 gene expression correlated with fertility rate (P<0.05). Age affected several semen quality parameters but did not affect HSP70 gene expression and protein abundance. The HSP70 gene molecule could be a biomarker that determines the fertility of Bali bulls.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230048"},"PeriodicalIF":1.7,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11095850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Chorionic Gonadotrophin (hCG) induces changes in IFN-pathway and Interferon-Stimulated Genes (ISGs) on the bovine endometrium at Day 18 of pregnancy.","authors":"Manuela Wolker Manta, Eduardo Pradebon da Silva, Suzana Rossato Feltrin, Amanda Luiza Prante, Karine de Vargas Aires, Leonardo Guedes de Andrade, Ana Paula da Silva, Carolina Dos Santos Amaral, Letícia Minussi Wink, Valério Marques Portela, Alfredo Quites Antoniazzi","doi":"10.1590/1984-3143-AR2023-0130","DOIUrl":"10.1590/1984-3143-AR2023-0130","url":null,"abstract":"<p><p>We hypothesized that the hCG modulates the expression of IFNT-pathway and ISGs in bovine endometrium during early pregnancy. The aim of the current study is to evaluate the effect of hCG on IFNT-pathway signals and ISGs expression in endometrial cells. For this, 29 non-lactating cross-bread cows were used in the study and submitted to a 9-day fixed-time artificial insemination (FTAI) protocol. The day of the AI was considered Day 0 (D0), and five days (D5) after the FTAI, the cows were allocated into two groups: Control and hCG group, when a hCG group received a single dose of 2.500UI of hCG. On day 18 after FTAI (D18) cows were slaughtered and endometrial tissue samples were collected. There was no difference between the embryo recovery rate of the cows in C compared to the hCG. The hCG group increased the accessory corpus luteum formation rate. The hCG resulted in greater serum progesterone concentration in the hCG group compared to the C on Day 14. Only the expression of IFNAR2 and STAT1 were upregulated on pregnant cows of the hCG group compared to the C group. The pathway genes (JAK1, STAT2, and IRF9) were not regulated. The mRNA abundance of ISG15, MX1, MX2, and OAS1 was upregulated in pregnant cows for hCG group, compared to C group. The results show that the administration of hCG, 5 days after AI, in addition to increasing the serum progesterone, modulates the expression of IFNT-pathway and ISGs on bovine endometrium on Day 18 of pregnancy.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 1","pages":"e20230130"},"PeriodicalIF":1.7,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10984569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140334445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of prenatal corticosteroid therapy on neonatal vitality and utility as a labor-inducing agent in Santa Inês ewes.","authors":"Elisiane Sateles Dos Santos, Rodrigo Freitas Bittencourt, Gleice Mendes Xavier, Carmo Emanuel Almeida Biscarde, Isabella de Matos Brandão Carneiro, Mateus Martins Rodrigues Dos Santos, Antonio de Lisboa Ribeiro","doi":"10.1590/1984-3143-AR2022-0109","DOIUrl":"10.1590/1984-3143-AR2022-0109","url":null,"abstract":"<p><p>Since the 1970s, maternal corticosteroid therapy has been used successfully to induce labor. This allows for better monitoring of parturients and provision of first aid to neonates, improving neonatal viability, as this treatment induces maturation in a variety of fetal tissues, thereby reducing morbidity and mortality. Although the effects of corticosteroids are well known, few studies have investigated the influence of this therapy in Santa Inês sheep. This study aimed to evaluate the efficacy of dexamethasone at two doses (8 and 16 mg) to induce lambing in Santa Inês ewes at 145 days of gestation and assess its effects on neonatal vitality. For this study, 58 ewes raised in an extensive system were investigated. Pregnancy was confirmed after artificial insemination at a set time or after controlled mounting. Ewes were separated into three groups: an untreated control group (G1: 0 mg) and groups treated with two doses of dexamethasone (G2: 8 mg and G3: 16 mg). In total, 79 lambs were born. Their vitality was assessed based on their Apgar score, weight, temperature, and postnatal behavior. SAS v9.1.3 (SAS Institute, Cary, NC) was used to analyze data, considering a 5% significance level for all analyses. The births in the induced groups occurred 48.4 ± 22.1 h after induction, while the ewes that underwent non-induced labor gave birth 131.96 ± 41.9 h after placebo application (p < 0.05), confirming the efficacy of dexamethasone to induce and synchronize labor. The induced and non-induced neonates had similar Apgar scores, temperatures, weights, and postnatal behavioral parameters (p > 0.05). This study showed that inducing labor in Santa Inês ewes at 145 days of gestation with a full (16 mg) or half dose (8 mg) of dexamethasone is an effective technique and does not compromise neonate vitality.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 1","pages":"e20220109"},"PeriodicalIF":1.7,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10984568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140334446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Padi6 expression patterns in buffalo oocytes and preimplantation embryos.","authors":"Qinqiang Sun, Yuan Yang, Yue Zhang, Dongrong Chen, Haiying Zheng, Guangsheng Qin, Qiang Fu","doi":"10.1590/1984-3143-AR2023-0146","DOIUrl":"10.1590/1984-3143-AR2023-0146","url":null,"abstract":"<p><p>The subcortical maternal complex, which consists of maternal-effect genes, plays a crucial role in the development of oocytes and preimplantation embryo until the activation of the zygote genome. One such gene, known as peptidyl-arginine deiminase VI (<i>Padi6</i>), is involved in the oocyte maturation, fertilization and embryonic development. However, the precise function of <i>Padi6</i> gene in buffalo is still unclear and requires further investigation. In this study, the sequence, mRNA and protein expression patterns of <i>Padi6</i> gene were analyzed in oocytes, preimplantation embryos and somatic tissues of buffalo. The coding sequence of gene was successfully cloned and characterized. Real-time quantitative PCR results indicated an absence of <i>Padi6</i> transcripts in somatic tissues. Notably, the expression levels of <i>Padi6</i> in oocytes showed an increased from the germinal vesicle stage to metaphase II stage, followed by a rapid decrease during the morula and blastocyst stages. Immunofluorescence analysis confirmed these findings, revealing a noticeable decline in protein expression levels. Our research provides the initial comprehensive expression profile of <i>Padi6</i> in buffalo oocytes and preimplantation embryos, serving as a solid foundation for further investigations into the functionality of maternal-effect genes in buffalo.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 1","pages":"e20230146"},"PeriodicalIF":1.7,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10984561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140334447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}