Successful transposition of sperm freezing, in vitro fertilization and artificial insemination methodologies in the Tadjik markhor species (Capra falconeri heptneri).
IF 1.6 4区 农林科学Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Yann Locatelli, Colin Vion, Nicolas Duffard, Nicolas Bon, Jérémy Bernard, Charly Binaud, Nina Trontti, Gérard Baril, Pascal Mermillod
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引用次数: 0
Abstract
The objective of the present study was to transpose sperm freezing methodology from domestic goat to the Tadjik markhor (Capra falconeri heptneri) and to address the feasibility to develop IVP and artificial insemination using such frozen semen. Semen of different adult markhor males were successfully recovered by electro-ejaculation and were then frozen using caprine methodology. Frozen semen showed good survival rates at thawing and good fertility rates were assessed in heterologous in vitro fertilization system with goat oocytes. LOPU/IVF was applied for Tadjik markhor females allowing the first successful blastocyst production in vitro. In an applied program, we also transposed successfully intrauterine AI method with frozen/thawed semen to the Tadjik markhor.
期刊介绍：
Animal Reproduction (AR) publishes original scientific papers and invited literature reviews, in the form of Basic Research, Biotechnology, Applied Research and Review Articles, with the goal of contributing to a better understanding of phenomena related to animal reproduction.
The scope of the journal applies to students, researchers and practitioners in the fields of veterinary, biology and animal science, also being of interest to practitioners of human medicine. Animal Reproduction Journal is the official organ of the Brazilian College of Animal Reproduction in Brazil.
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