Analytical Sciences最新文献

{"title":"Evaluation of AgFON-based SERS combined with various headspace methods for the detection of thiol compounds in wine","authors":"Kosuke Watanabe,&nbsp;Hiroyuki Takei","doi":"10.1007/s44211-026-00898-x","DOIUrl":"10.1007/s44211-026-00898-x","url":null,"abstract":"<div>\u0000 \u0000 <p>The thiol compounds present in wine 4-mercapto-4-methyl-2-pentanone (4MMP), 3-mercapto-1-hexanol (3MH), and 3-mercaptohexyl acetate (3MHA) are key components contributing to the aromatic characteristics of wines such as Sauvignon Blanc, giving muscat and blackcurrant notes. This study evaluated the effectiveness of surface-enhanced Raman spectroscopy (SERS) using silver-film over nanosphere (AgFON) substrate combined with immersion, static headspace, and dynamic headspace methods. The immersion method was found unsuitable for detection of target molecules in low concentrations due to interference from wine-derived components. In contrast, detection was difficult in the gas-phase conditions with the static headspace method, whereas the introduction of an acceptor phase under liquid-phase conditions facilitated spectral observation of the target compounds. Furthermore, the headspace method with dynamic control of the acceptor demonstrated the detectability of 4MMP, with characteristic spectral features observable at 10 nM under the examined experimental conditions. For 3MH and 3MHA, peaks overlapping with other wine components remained an issue. Principal component analysis (PCA) was applied as an exploratory tool to visualize spectral variance, and the results showed partial cluster overlap under certain conditions, while revealing trends that differentiate thiol-containing samples from wine-derived backgrounds. These results indicate that the headspace SERS approach using AgFON combined with an acceptor phase has potential for the detection of thiol compounds in wine, particularly for 4MMP.</p>\u0000 <span>AbstractSection</span>\u0000 Graphical abstract\u0000 <div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div>\u0000 \u0000 </div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 6","pages":"377 - 388"},"PeriodicalIF":2.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward non-enzymatic glucose sensing based on organic electronics: organic field-effect transistors with multifunctionalized extended-gate electrodes","authors":"Yui Sasaki,&nbsp;Muthukumar Govindaraj,&nbsp;Tsuyoshi Minami","doi":"10.1007/s44211-026-00892-3","DOIUrl":"10.1007/s44211-026-00892-3","url":null,"abstract":"<div><p>Accurate glucose analysis has been a major research focus for the past five decades since the pioneering development of enzymatic glucose sensing, driven by the global demand for rapid and reliable diagnostics. In contrast to well-established enzymatic glucose sensors, the development of chemical sensors based on supramolecular receptors remains a developing area. Among the supramolecular receptors for glucose, phenylboronic acid (PBA) derivatives provide unique functions, such as the formation of boronate esters with glucose and their reversibility. In this study, we focus on organic field-effect transistors (OFETs) combined with PBA derivatives as chemical sensors. Although the beneficial properties of OFETs have been explored for physical sensor applications in organic electronics, the instability of organic devices remains a major limitation for chemical sensing applications. To address this issue, an extended-gate structure was employed as the OFET-based chemical sensor configuration. In this sensor design, an extended-gate electrode functionalized with a PBA derivative self-assembled monolayer was connected to the gate electrode of the OFET, enabling glucose detection via boronate ester formation at the aqueous-sensing electrode interface. In this mini-review, we introduce strategies for establishing non-enzymatic glucose sensors using OFETs with multifunctionalized extended-gate electrodes. Notably, selective glucose detection in human blood plasma has been demonstrated by combining a two-dimensional material that suppresses the physical adsorption of endogenous proteins onto an extended-gate electrode. Finally, an approach that integrates a microfluidic chamber into an extended-gate-based OFET device is introduced to highlight the inherent reversibility of the PBA derivative.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 7","pages":"491 - 499"},"PeriodicalIF":2.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-026-00892-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical sensing based on electrochemiluminescence","authors":"Takahashi Fumiki","doi":"10.1007/s44211-026-00884-3","DOIUrl":"10.1007/s44211-026-00884-3","url":null,"abstract":"","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 4","pages":"197 - 199"},"PeriodicalIF":2.0,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and electrochemical properties of a novel thiophene–sulfamerazine Schiff base containing scaffold: comprehensive investigation of its interaction with DNA via voltammetric, spectrophotometric, and molecular docking studies","authors":"Nida Nur Adiyan,&nbsp;Abdulkadir Levent,&nbsp;Mehmet Sönmez","doi":"10.1007/s44211-026-00895-0","DOIUrl":"10.1007/s44211-026-00895-0","url":null,"abstract":"<div><p>This study reports the synthesis and comprehensive characterization of a new Schiff-base ligand (L), derived from the condensation of 2,5-thiophenedicarboxaldehyde and sulfamerazine. The structural identity of L was unequivocally confirmed through elemental analysis, FT-IR, ¹H/¹³C NMR spectroscopy, and LC-MS/MS measurements. The electrochemical behavior of L was investigated on a glassy carbon electrode in an anhydrous medium, revealing an irreversible, diffusion-controlled redox process best described by an electrochemical–chemical mechanism. The interaction between L and DNA was examined using voltammetric and spectrophotometric techniques. Differential pulse voltammetry demonstrated a pronounced decrease (44.4%) in the guanine oxidation signal upon interaction with L, indicating strong binding with an estimated binding constant of 1.04 × 10⁶ M^–1. Complementary UV–Vis studies showed a hypochromic effect accompanied by a hypsochromic shift in the ligand’s absorption spectrum, consistent with an intercalative binding mode, yielding a Kb value of 1.44 × 10⁶ M^–1. Molecular docking simulations supported the experimental findings by revealing that L preferentially binds within the minor groove of DNA. The interaction is stabilized through a network of noncovalent forces, including conventional hydrogen bonds, van der Waals interactions, C–H···X contacts, and π–sulfur contributions, with a calculated binding energy of − 8.8 kcal mol^− 1. Overall, the combined electrochemical, spectroscopic, and theoretical results demonstrate that the synthesized Schiff-base ligand L exhibits high affinity toward DNA, positioning it as a promising candidate for further pharmacological evaluation.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 5","pages":"341 - 351"},"PeriodicalIF":2.0,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147502736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiently analyzing lithium cobalt oxide using gas-generating reaction assisted headspace GC","authors":"Wei-Qi Xie,&nbsp;Zi-Fan Lan,&nbsp;Yi-Xian Gong","doi":"10.1007/s44211-026-00872-7","DOIUrl":"10.1007/s44211-026-00872-7","url":null,"abstract":"<div><p>This study introduces an innovative gas-generating reaction assisted headspace approach for precise quantification of lithium cobalt oxide using headspace GC. The approach utilizes gas chromatographic measurement of carbon dioxide generated through redox reaction between the analyte and oxalic acid in acidified sealed vials. Method validation demonstrated reliable analytical performance, with high precision and acceptable recovery rates in spiked samples. The automated headspace GC technique provides substantial benefits for the rapid quality control of lithium cobalt oxide products. Additionally, this gas-evolution based strategy establishes a framework for indirect determination methods through headspace analysis.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 5","pages":"313 - 318"},"PeriodicalIF":2.0,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on direct PCR and its applications","authors":"Ting Xu,&nbsp;Siyu Yang,&nbsp;Yingqiu Pan,&nbsp;Xingjuan Hu,&nbsp;Feng Guan,&nbsp;Huili Xia","doi":"10.1007/s44211-026-00893-2","DOIUrl":"10.1007/s44211-026-00893-2","url":null,"abstract":"<div>\u0000 \u0000 <p>Direct Amplification Technology (Direct PCR) is derived from conventional PCR and eliminates the cumbersome DNA extraction process, thereby enhancing detection efficiency and accuracy. Combined with evolving PCR-derived techniques, it has found extensive applications across multiple fields. This article provides an overview of the development process, fundamental principles, basic procedures, and derivative technologies of direct PCR. It further details its applications in testing various samples including blood, microbial specimens, and cellular/tissue samples, offering foundational knowledge to support the popularization and further advancement of this technology.</p>\u0000 <span>AbstractSection</span>\u0000 Graphical Abstract\u0000 <div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div>\u0000 \u0000 </div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 5","pages":"289 - 297"},"PeriodicalIF":2.0,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of infrared desolvation device for single cell element analysis using droplet sample injection inductively coupled plasma spectrometry","authors":"Kai Fukuchi,&nbsp;Syu Yamaji,&nbsp;Yuya Shimizu,&nbsp;Takashi Ohta,&nbsp;Akane Yaida,&nbsp;Yuki Maemoto,&nbsp;Motohide Aoki,&nbsp;Tomonari Umemura,&nbsp;Akitoshi Okino","doi":"10.1007/s44211-026-00888-z","DOIUrl":"10.1007/s44211-026-00888-z","url":null,"abstract":"<div><p>Microdroplet injection systems, which introduce single cells into an inductively coupled plasma by encapsulating them in droplets, have been developed by several research groups, including our group. In our laboratory, to improve the analytical sensitivity, a droplet desolvation system using a ribbon heater has achieved an approximately tenfold improvement in sensitivity. However, the desolvation throughput was limited to 100 Hz because of the low heat-transfer efficiency associated with indirect of indirect heating via heated gas. In this paper, a novel desolvation device using infrared radiation was developed to improve the desolvation throughput. Infrared radiation enables direct and efficient droplet heating. The developed device comprises a heating section and a cooling section. An infrared lamp with a luminous length of 140 mm was used in the heating section. Infrared radiation was focused on the flight axis of the droplets to enhance irradiation efficiency. The cooling section was placed downstream of the heating section to condense and remove vapor. With a power of 1000 W applied to the lamp and a carrier gas flow rate of 0.1 L min⁻¹, the carrier gas temperatures at the bottom of the heating section remained below 71 °C for both argon and helium. Under these conditions, the cells remained intact without rupture. Helium exhibited superior throughput compared to argon and when the carrier gas flow rate was above 0.5 L min⁻¹, the desolvation at a throughput of 1000 Hz could be achieved with an applied power of 150 W to the lamp.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 5","pages":"319 - 328"},"PeriodicalIF":2.0,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-026-00888-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated microfluidic system for automatic and self-validated analysis of cervical extracellular vesicle markers PD-L1 and ERBB3","authors":"Yunxing Lu,&nbsp;Han Qin,&nbsp;Wenjing Zhang,&nbsp;Qiang Shi,&nbsp;Jianan Hui,&nbsp;Zhenhua Wu,&nbsp;Yiman Song,&nbsp;Xiaoyue Yang","doi":"10.1007/s44211-026-00871-8","DOIUrl":"10.1007/s44211-026-00871-8","url":null,"abstract":"<div><p>The early and precise diagnosis of gynecological malignancies, such as cervical cancer, is critical for improving patient treatments. Extracellular vesicles (EVs), such as exosomes, which carry molecular signals from their parental cells, offer a promising method for non-invasive liquid biopsy, however, conventional detection methods are often complex, high in reagent consumption, and susceptible to environmental fluctuations. To address this, we present an integrated, self-validated microfluidic system for the rapid, on-chip isolation and multiplexed identification of the gynecological EV markers PD-L1 and ERBB3. The chip achieved simultaneous on-chip processing of test and positive samples for parallel analysis within 1 h, enabling synchronous detection under the same conditions and thereby significantly enhancing the reliability of the assay. Additionally, a deep learning YOLOv8-based self-validated detection strategy facilitates automated and precise fluorescence identification. Validation with four cell lines (SiHa, C33A, HeLa, and H8) revealed remarkable EV protein signatures, achieving a limit of detection (LOD) of 15.56 particles/μL. This platform provides an integrated tool for sensitive and precise EV marker analysis, holding prospective potential for the early screening and personalized therapy guidance of gynecological tumor detection.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div><p>Integrated analytical system for one-stop and self-validated exosome complex formation and multiplex tumor fingerprint analysis by deep learning. Exosome samples were immuno-isolated and labeled with probes, followed by monodispersed among the particular arrays for deep learning model YOLOv8-based positional migration and identification automatically. Four kinds of samples were measured and remarkable differences were acquired, the two tumor progressions: immune evasion and proliferative signaling were revealed. The integrated system is pospective for sensitive, easy-handing and automatic exosome markers analysis in POCT field.</p></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 5","pages":"299 - 312"},"PeriodicalIF":2.0,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-026-00871-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147466596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of ligand alkyl chain length on lanthanide extraction using liposomes incorporating diglycolamic acid","authors":"Takeru Uehara,&nbsp;Shinya Yamasaki,&nbsp;Sota Shimizu,&nbsp;Yuichi Takaku,&nbsp;Yudai Shigekawa,&nbsp;Aya Sakaguchi","doi":"10.1007/s44211-026-00889-y","DOIUrl":"10.1007/s44211-026-00889-y","url":null,"abstract":"<div><p>This study introduces an environmentally benign liposome-based extraction system that selectively separates and recovers lanthanides (Ln) without requiring an organic solvent. Recovery of Ln, particularly from high-level radioactive liquid waste streams, is critical for resource utilization and reducing long-term radiotoxicity. The proposed system incorporates highly Ln-selective diglycolamic acid (DGAA) ligands into phospholipid liposomes. Fluorescence spectroscopy demonstrated that europium (Eu) adsorption, mediated by the hydrophilic head group of the incorporated DGAA ligands, reached equilibrium almost immediately upon mixing. Inductively coupled plasma–mass spectrometry revealed that the adsorption efficiency increased with increasing concentration of incorporated DGAA. Approximately three DGAA ligands are required to adsorb one Eu(III) ion. The selectivity for heavy Ln was comparable to that of conventional DGAA-based solvent-extraction systems and was found to be independent of DGAA alkyl chain length. In contrast, the incorporation stability depended on alkyl chain length; for example, liposomes containing <i>N</i>,<i>N</i>-didodecyldiglycolamic acid retained the ligand without significant precipitation or fluorescence decrease for 7 days at concentrations up to 237 µM/g-egg lecithin. These findings establish liposome-based extraction as a flexible platform in which the ligand head group governs the adsorption characteristics while the alkyl chain length dictates the incorporation stability, providing a quantitative foundation for future solvent-free separation technologies.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 4","pages":"269 - 279"},"PeriodicalIF":2.0,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13013227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147455075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional synergistic action of metal–organic framework composites enhance the electrochemical detection sensitivity of dopamine","authors":"Yu Bai,&nbsp;Jine Wang,&nbsp;Shuang Han,&nbsp;Zhichao Zhang,&nbsp;Yuewu Zhao","doi":"10.1007/s44211-026-00882-5","DOIUrl":"10.1007/s44211-026-00882-5","url":null,"abstract":"<div><p>Metal–organic frameworks (MOFs) have shown major promise for electrochemical dopamine (DA) sensing owing to their high surface area, tunable pores, and abundant active sites. In this work, iron-tetra(4-carboxyphenyl)porphyrin-coordinated assembly metal–organic framework (Fe-TCPP MOF material) capable of effectively catalyzing the oxidation of DA was synthesized. Subsequently, it was combined with reduced graphene oxide (rGO) with excellent electrical conductivity and perfluorosulfonic acid polymer (Nafion) with cation selectivity to construct an electrochemical sensor that could synergistically enhance the sensitivity of DA detection. The resulting Fe-TCPP/rGO/Nafion electrode detected DA from 0.1 to 3200 μM with a detection limit of 0.021 μM, outperforming most reported sensors. It also maintained high stability, selectivity, and interference resistance over repeated cycles, and achieved recoveries of 84.84–110.76% in real samples. Mechanistically, the porous Fe-TCPP MOF increased accessibility of catalytic sites; rGO improved charge transport and π–π adsorption; and Nafion provided cation-exchange selectivity and electrostatic preconcentration. These complementary roles yielded synergistic gains in catalytic oxidation, preconcentration, selectivity, and anti-interference. The integrated strategy offered a practical route to high-performance MOF-based DA sensors and a useful reference for developing efficient analytical materials.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 4","pages":"245 - 255"},"PeriodicalIF":2.0,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147442083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}