{"title":"StageTip: a little giant unveiling the potential of mass spectrometry-based proteomics.","authors":"Eisuke Kanao, Yasushi Ishihama","doi":"10.1007/s44211-025-00749-1","DOIUrl":null,"url":null,"abstract":"<p><p>This review highlights the growing impact of StageTips (Stop and Go Extraction Tips), a pipette tip-based LC column in MS-based proteomics. By packing standard pipette tips with reversed-phase, ion-exchange, or metal oxide materials, StageTips enable efficient peptide desalting, fractionation, selective enrichment, and in-tip reactions with minimal sample loss. Recent improvements, including new resin designs and integrated workflows, have further expanded the applications to phosphoproteomics, protein terminomics, and single-cell proteomics. With their simplicity, high reproducibility, and low cost, StageTips offer a versatile platform that can be seamlessly integrated into automated pipelines, increasing the throughput and the depth of proteome analysis. As materials and protocols continue to evolve, StageTips will continue to develop as an essential keystone for robust sample preparation in next-generation proteomics research.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Sciences","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s44211-025-00749-1","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
This review highlights the growing impact of StageTips (Stop and Go Extraction Tips), a pipette tip-based LC column in MS-based proteomics. By packing standard pipette tips with reversed-phase, ion-exchange, or metal oxide materials, StageTips enable efficient peptide desalting, fractionation, selective enrichment, and in-tip reactions with minimal sample loss. Recent improvements, including new resin designs and integrated workflows, have further expanded the applications to phosphoproteomics, protein terminomics, and single-cell proteomics. With their simplicity, high reproducibility, and low cost, StageTips offer a versatile platform that can be seamlessly integrated into automated pipelines, increasing the throughput and the depth of proteome analysis. As materials and protocols continue to evolve, StageTips will continue to develop as an essential keystone for robust sample preparation in next-generation proteomics research.
这篇综述强调了StageTips （Stop and Go Extraction Tips）在MS-based蛋白质组学中日益增长的影响，StageTips是一种基于移液头的LC柱。通过用反相、离子交换或金属氧化物材料包装标准移液头，StageTips能够有效地进行肽脱盐、分馏、选择性富集和针尖内反应，样品损失最小。最近的改进，包括新的树脂设计和集成的工作流程，进一步扩展了磷蛋白质组学、蛋白质术语组学和单细胞蛋白质组学的应用。凭借其简单性、高重复性和低成本，StageTips提供了一个多功能平台，可以无缝集成到自动化管道中，提高了蛋白质组分析的吞吐量和深度。随着材料和方案的不断发展，StageTips将继续发展成为下一代蛋白质组学研究中稳健样品制备的重要基石。
期刊介绍：
Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods.
This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.
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