Analytical Sciences最新文献

Development of a portable battery-powered total reflection X-ray fluorescence spectrometer.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-27 DOI: 10.1007/s44211-025-00751-7

Hiroshi Yoshii, Tsugufumi Matsuyama, Hiroki Nagai, Yasuhiro Sakai

引用次数: 0

StageTip: a little giant unveiling the potential of mass spectrometry-based proteomics.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-26 DOI: 10.1007/s44211-025-00749-1

Eisuke Kanao, Yasushi Ishihama

引用次数: 0

Current advances in separation chemistry for antibody purification and analysis.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-24 DOI: 10.1007/s44211-025-00748-2

Suprit Deol, Yutaka Matsuda, Yuki Hiruta

{"title":"Current advances in separation chemistry for antibody purification and analysis.","authors":"Suprit Deol, Yutaka Matsuda, Yuki Hiruta","doi":"10.1007/s44211-025-00748-2","DOIUrl":"https://doi.org/10.1007/s44211-025-00748-2","url":null,"abstract":"Monoclonal antibodies (mAbs) have become essential in modern therapeutics, offering high specificity and efficacy in treating diseases such as cancer, autoimmune disorders, and infectious diseases. With the increasing demand for mAbs, robust analytical and purification technologies are critical to ensuring their safety, efficacy, and consistency. This review highlights recent advances in the application of HPLC for both mAb analysis and purification. In the analytical domain, techniques such as size exclusion chromatography (SEC), hydrophobic interaction chromatography (HIC), ion exchange chromatography (IEX), and reversed-phase chromatography (RPLC) are explored for their role in evaluating mAb structural attributes and post-translational modifications (PTMs). These methods are indispensable for ensuring stability and functionality while meeting stringent regulatory requirements. In the context of purification, Protein A affinity chromatography remains the gold standard for initial capture of mAb; however, challenges such as high cost and harsh elution conditions have prompted the development of alternative purification technologies. IEX, HIC, multimodal, and membrane chromatography are also critical in achieving high-purity mAb products through multistep workflows, including intermediate purification and polishing stages. This review emphasizes the central role of HPLC in addressing the complex challenges of mAb manufacturing and characterization. By integrating established and emerging chromatographic techniques, it provides insights into the future directions of therapeutic antibody development.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Confirmation of radicals auto-generated in [Bmim][PF₆]/[C₁₆mim][PF₆]/water reverse micelles and the radical quantification based on the 1,1-diphenyl-2-picrylhydrazine (DPPHH) spectroscopic probe.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-20 DOI: 10.1007/s44211-025-00744-6

Mengge Wang, Xiaofeng Chen, Zhigang Wang, Ming Li

{"title":"Confirmation of radicals auto-generated in [Bmim][PF6]/[C16mim][PF6]/water reverse micelles and the radical quantification based on the 1,1-diphenyl-2-picrylhydrazine (DPPHH) spectroscopic probe.","authors":"Mengge Wang, Xiaofeng Chen, Zhigang Wang, Ming Li","doi":"10.1007/s44211-025-00744-6","DOIUrl":"https://doi.org/10.1007/s44211-025-00744-6","url":null,"abstract":"Ionic liquids (ILs) which belong to the molten salt of a high ionic environment exhibit a number of unique properties, including the formation of various heterogeneities in their microemulsion. We found that auto-generated radicals within a [Bmim][PF6]/[C16mim][PF6]/PBS reverse micelles (IL-RMs) and then the radicals were quantified in this study. For the radical confirmation, EPR spectroscopy with 5,5-dimethyl-1-pyrroline N-oxide (DMPO) free radical scavenger was carried out. A spectroscopic chemical probe of 1,1-diphenyl-2-picrylhydrazine (DPPHH) was employed to quantify the radicals in the IL-RM systems. The mechanism of the radical generation was also proposed. The DPPHH probe method is simple, rapid, and sensitive to quantify free radicals in the IL-RM systems. Finally, IL-RMs were successfully applied to degrade Rhodamine B (Rho-B) dye with low degradation cost, simple operation, short time consumption, and remarkable degradation effect. The results show a near 100% degradation rate for 10 μmol·L-1 Rho-B in the IL-RMs system with pH 7.4 PBS containing 1 mmol·L-1 chloroacetic acid as an aqueous phase.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring liquid-liquid phase separation in vitro and in vivo using multimodal nonlinear optical imaging.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-20 DOI: 10.1007/s44211-025-00747-3

Yusuke Murakami, Mia Obuchi, Hiroshi Kamizawa, Shinichi Miyazaki, Akihiro Kishimura, Ryosuke Oketani, Kotaro Hiramatsu, Philippe Leproux, Yu Hayashi, Kentaro Shiraki, Hideaki Kano

引用次数: 0

Electrochemically active DNA ligands for gene detection: present and future.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-19 DOI: 10.1007/s44211-025-00745-5

Shigeori Takenaka, Shinobu Sato

{"title":"Electrochemically active DNA ligands for gene detection: present and future.","authors":"Shigeori Takenaka, Shinobu Sato","doi":"10.1007/s44211-025-00745-5","DOIUrl":"https://doi.org/10.1007/s44211-025-00745-5","url":null,"abstract":"Electrochemical gene sensing methods are gaining attention as diagnostic chips. Here, we review the electrochemically active DNA ligand-based sensing methods. Various DNA ligands have been reported in these studies, among which metal complexes, methylene blue, and ferrocenyl naphthalene diimide (FND) have been studied in detail. DNA probe immobilized electrodes have been created, hybridization reactions on the electrodes with target DNA fragments have been performed, and electrochemical gene detection has been possible using these DNA ligands. An example of the realization of this system is the successful and accurate cancer diagnosis using FND to examine abnormal methylation of the hTERT gene, providing reassurance about the system's reliability. In addition, electrochemical detection of PCR products has been realized using the current decrease due to the double-stranded DNA binding of methylene blue although it is a signal-off system. A naphthalene diimide derivative with ferrocene and β-CD, FNC, increased the current upon double-stranded DNA binding. Using these FNCs, the detection of PCR products in a homogeneous system was realized. Electrochemical qPCR was realized with these ligands. Since FNDs also bind strongly to tetraplex or G-quadruplex (G4) DNA, we succeeded in electrochemically detecting telomerase activity, which is known as a cancer marker, using FNDs to detect the amount of telomeric DNA elongation, which is its substrate, as the amount of G4 DNA. This technique has realized compassionate cancer diagnosis from oral swab fluid. It is known that G4 is also present in viral genome RNA, and a viral testing method using G4 is expected to be a potential alternative to PCR. The first example was the electrochemical detection of novel coronaviruses using incFND as an RNA G4 ligand.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing an optical sensor with exceptional sensitivity and specificity for the detection of ultra-trace amounts of boron

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-14 DOI: 10.1007/s44211-025-00737-5

Abdullah H. Alluhayb, Ahmed H. Alanazi, Alaa M. Younis, Refat El-Sayed, Khaled F. Debbabi, Alaa S. Amin

{"title":"Designing an optical sensor with exceptional sensitivity and specificity for the detection of ultra-trace amounts of boron","authors":"Abdullah H. Alluhayb, Ahmed H. Alanazi, Alaa M. Younis, Refat El-Sayed, Khaled F. Debbabi, Alaa S. Amin","doi":"10.1007/s44211-025-00737-5","DOIUrl":"10.1007/s44211-025-00737-5","url":null,"abstract":"<div>A specialized optical chemical sensor for boron detection was designed using a polymer inclusion membrane (PIM). This unique sensor relies on an encapsulation method to create its optical membrane. The components involved in this sensor include azomethine H (AMH) as the reactive agent, polyvinyl chloride as the foundational polymer, and dinonylnaphthalene sulphonic acid (DNNS) as the extraction agent within the PIM structure. Various parameters, such as membrane thickness, concentration of AMH and DNNS, plasticizer proportions, stirring dynamics, and the pH of the solution under study, significantly affected the sensor’s performance. Within a detection range spanning 4.0–116 ng mL−1 of boron, the sensor demonstrated a strong linear relationship with detection and quantification thresholds of 1.25 and 3.94 ng mL−1, respectively. The peak absorption wavelength (λmax) for this PIM-based sensor was identified at 424 nm. Moreover, the sensor displayed a reproducibility (RSD) of 1.65% and a repeatability RSD of 1.43%, coupled with a swift response time of approximately 3.0 min. The membrane selectivity was tested against boron in acidic environments. This proposed sensor effectively detected boron in diverse samples such as water, vegetation, soil, and ceramic samples, with findings corroborated by an AAS technique.<h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div><div>Schematic representation for the preparation, and complexation of the formed sensor and B‏‏–AMH–DNNS complex</div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 4","pages":"473 - 486"},"PeriodicalIF":1.8,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solvent properties of ionic liquids on complex formation of Ni(II) ion with molecular liquids.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-14 DOI: 10.1007/s44211-025-00741-9

Toshiyuki Takamuku

{"title":"Solvent properties of ionic liquids on complex formation of Ni(II) ion with molecular liquids.","authors":"Toshiyuki Takamuku","doi":"10.1007/s44211-025-00741-9","DOIUrl":"https://doi.org/10.1007/s44211-025-00741-9","url":null,"abstract":"Room-temperature ionic liquids (ILs) attract much attention as green solvents because of their negligible volatility and non-flammability. In analytical chemistry, the application of ILs to chemical separation and solvent extraction has been investigated. However, there have been a smaller number of reports on the mechanisms of chemical equilibria in the solvents of ILs at a molecular level. In this review, the series of our investigations on the complex formation of Ni2+ ion with molecular liquids (MLs) in imidazolium-based ILs, 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)amide ([C2mim][TFSA]) and 1-methyl-3-octylimidazolium bis(trifluoromethylsulfonyl)amide ([C8mim][TFSA]) was summarized. The MLs involved methanol (MeOH), ethanol (EtOH), acetonitrile (AN), and dimethyl sulfoxide (DMSO). In [C2mim][TFSA], the Ni2+-ML complexes are stabler in the sequence of DMSO >> AN > EtOH ≈ MeOH complexes, which almost depends on the electron donicities of MLs. In contrast, in [C8mim][TFSA], the sequence changes to AN > DMSO > EtOH > MeOH complexes, despite of the lowest electron donicity of AN. Thus, only the electron donicities of MLs do not decide the stability of the complexes. The reasons for the stabilities of the Ni2+-ML complexes were interpreted in terms of the microscopic interactions between ML and the ILs' cation observed by the infrared (IR) and 1H and 13C nuclear magnetic resonance (NMR) spectroscopic techniques. Furthermore, to clarify the effects of ML cluster formation on the complex formation mechanisms, the heterogeneous mixing between IL and ML were quantitatively evaluated by small-angle neutron scattering (SANS) at the mesoscopic scale.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification techniques of soil organic carbon: an appraisal.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-11 DOI: 10.1007/s44211-025-00746-4

Avinash Kanagaraj, Sathiya Bama Kaliappan, Thenmozhi Shanmugam, Bharani Alagirisamy, Kumaraperumal Ramalingam

{"title":"Quantification techniques of soil organic carbon: an appraisal.","authors":"Avinash Kanagaraj, Sathiya Bama Kaliappan, Thenmozhi Shanmugam, Bharani Alagirisamy, Kumaraperumal Ramalingam","doi":"10.1007/s44211-025-00746-4","DOIUrl":"https://doi.org/10.1007/s44211-025-00746-4","url":null,"abstract":"This review provides an overview of the analytical methods utilized across laboratory, field, landscape, and regional scales for assessing soil organic carbon (SOC) in agricultural soils. The significance of soil depth in SOC estimation underscores the importance of selecting appropriate sampling designs, soil depths, analytical methods, and baseline selection methods for accurate soil carbon stock estimation. Traditional methods such as wet digestion and dry combustion (DC) remain prevalent in routine laboratory analysis, with DC considered the standard reference method, surpassing wet digestion in accuracy and reliability. Recent advancements in spectroscopic techniques enable SOC measurement both in laboratory settings and in situ, even at greater depths. Aerial spectroscopy, which employs multispectral and hyperspectral sensors, unmanned aerial vehicles (UAVs), or satellites, facilitates surface SOC measurement. While the current precision levels of these techniques may be limited, forthcoming hyperspectral sensors with enhanced signal‒to‒noise ratios are expected to significantly increase the prediction accuracy. Furthermore, at the global level, satellite remote sensing techniques have considerable potential for SOC estimation. Regardless of whether traditional or novel approaches are utilized, the selection of SOC determination depends on available resources and research requirements, each of which plays a distinct role in soil carbon and climate research. This paper provides an overview of various scale-dependent techniques for measuring SOC in agricultural soil, along with its potential limitations.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional metal complex systems emerging from analytical chemistry for theranostic applications.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-11 DOI: 10.1007/s44211-025-00740-w

Nobuhiko Iki

{"title":"Multifunctional metal complex systems emerging from analytical chemistry for theranostic applications.","authors":"Nobuhiko Iki","doi":"10.1007/s44211-025-00740-w","DOIUrl":"https://doi.org/10.1007/s44211-025-00740-w","url":null,"abstract":"Metal complexes have long played a pivotal role in analytical chemistry due to their ability to detect and separate ions through the synergistic interaction between metal centers and ligands. This functionality can be further enhanced by integrating metal complexes non-covalently with various media, such as materials or separation platforms. Over the past four decades, the author has explored metal complex systems, including thiacalixarene-lanthanide(III) complexes, diradical platinum(II) complexes, and MOF-74, which exhibit a broad spectrum of functionalities spanning analytical applications to theranostics. Examples discussed in this review include luminescent supramolecular sensors for soft-metal ions, upconverting complexes, near-infrared light-absorbing probes for pH and hydrophobic cavities, magnetic resonance imaging (MRI), photoacoustic imaging, photothermal therapy, and neutron capture therapy. The multifunctionality of these systems arises from their cooperative, synergistic, and supramolecular nature, underpinned by non-covalent interactions among their components. Analytical chemistry has been, and will continue to be, a cornerstone for the discovery and development of such multifunctional metal complex systems.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0