Analytical Sciences最新文献

Correction: Recent advances in the development of ²²⁵Ac- and ²¹¹At-labeled radioligands for radiotheranostics.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-09 DOI: 10.1007/s44211-025-00763-3

Masayuki Munekane, Takeshi Fuchigami, Kazuma Ogawa

引用次数: 0

Detection of a large antigen through the masking and exposure of a fragment of split luciferase.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-08 DOI: 10.1007/s44211-025-00754-4

Cheng Qian, Ayumu Ninomiya, Natsuki Shibukawa, Hiroshi Ueda, Takanobu Yasuda, Bo Zhu, Tetsuya Kitaguchi

{"title":"Detection of a large antigen through the masking and exposure of a fragment of split luciferase.","authors":"Cheng Qian, Ayumu Ninomiya, Natsuki Shibukawa, Hiroshi Ueda, Takanobu Yasuda, Bo Zhu, Tetsuya Kitaguchi","doi":"10.1007/s44211-025-00754-4","DOIUrl":"https://doi.org/10.1007/s44211-025-00754-4","url":null,"abstract":"We developed PMBiT, an antibody-binding Protein M (PM)-based bioluminescent probe that detects large antigens via luciferase reconstitution by exposing a luciferase fragment. Detection is achieved by exploiting the principle that the antibody, large antigen, and PM cannot form a complex simultaneously. PMBiT was prepared by conjugating PM with a HiBiT-based peptide from split NanoLuc luciferase through an Azide-DBCO click reaction. It retained its binding activity to the antibody and showed bioluminescence upon reconstitution of the luciferase with LgBiT, the other fragment of the split NanoLuc. Mixing PMBiT with various IgG antibodies resulted in decreased bioluminescence. In contrast, when PMBiT was mixed with IgG bound to its large antigen, such as human C-reactive protein, a dose-dependent increase in bioluminescence was obtained. Molecular dynamics simulations of PM showed that two regions in the C-terminus contribute to steric clashes with antigens owing to their relatively rigid structures. Furthermore, in silico analysis of the structure suggested that the antigen size was the primary factor blocking the binding of PMBiT to IgG for antigen detection. An immunoassay utilizing PMBiT does not require genetic manipulation of antibodies, allowing for seamless and scalable antibody replacement, and will advance the future of on-site detection and rapid diagnostics.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosensing approaches in body fluids using extended-gate-type organic field-effect transistor enzymatic sensors.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-05 DOI: 10.1007/s44211-025-00750-8

Yui Sasaki, Tsuyoshi Minami

引用次数: 0

Recent advances in single-particle analysis with nanopore technology. 利用纳米孔技术进行单颗粒分析的最新进展。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-05 DOI: 10.1007/s44211-025-00757-1

Akihide Arima

引用次数: 0

Development of a portable battery-powered total reflection X-ray fluorescence spectrometer.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-27 DOI: 10.1007/s44211-025-00751-7

Hiroshi Yoshii, Tsugufumi Matsuyama, Hiroki Nagai, Yasuhiro Sakai

引用次数: 0

StageTip: a little giant unveiling the potential of mass spectrometry-based proteomics.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-26 DOI: 10.1007/s44211-025-00749-1

Eisuke Kanao, Yasushi Ishihama

引用次数: 0

Current advances in separation chemistry for antibody purification and analysis.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-24 DOI: 10.1007/s44211-025-00748-2

Suprit Deol, Yutaka Matsuda, Yuki Hiruta

{"title":"Current advances in separation chemistry for antibody purification and analysis.","authors":"Suprit Deol, Yutaka Matsuda, Yuki Hiruta","doi":"10.1007/s44211-025-00748-2","DOIUrl":"https://doi.org/10.1007/s44211-025-00748-2","url":null,"abstract":"Monoclonal antibodies (mAbs) have become essential in modern therapeutics, offering high specificity and efficacy in treating diseases such as cancer, autoimmune disorders, and infectious diseases. With the increasing demand for mAbs, robust analytical and purification technologies are critical to ensuring their safety, efficacy, and consistency. This review highlights recent advances in the application of HPLC for both mAb analysis and purification. In the analytical domain, techniques such as size exclusion chromatography (SEC), hydrophobic interaction chromatography (HIC), ion exchange chromatography (IEX), and reversed-phase chromatography (RPLC) are explored for their role in evaluating mAb structural attributes and post-translational modifications (PTMs). These methods are indispensable for ensuring stability and functionality while meeting stringent regulatory requirements. In the context of purification, Protein A affinity chromatography remains the gold standard for initial capture of mAb; however, challenges such as high cost and harsh elution conditions have prompted the development of alternative purification technologies. IEX, HIC, multimodal, and membrane chromatography are also critical in achieving high-purity mAb products through multistep workflows, including intermediate purification and polishing stages. This review emphasizes the central role of HPLC in addressing the complex challenges of mAb manufacturing and characterization. By integrating established and emerging chromatographic techniques, it provides insights into the future directions of therapeutic antibody development.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Confirmation of radicals auto-generated in [Bmim][PF₆]/[C₁₆mim][PF₆]/water reverse micelles and the radical quantification based on the 1,1-diphenyl-2-picrylhydrazine (DPPHH) spectroscopic probe.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-20 DOI: 10.1007/s44211-025-00744-6

Mengge Wang, Xiaofeng Chen, Zhigang Wang, Ming Li

{"title":"Confirmation of radicals auto-generated in [Bmim][PF6]/[C16mim][PF6]/water reverse micelles and the radical quantification based on the 1,1-diphenyl-2-picrylhydrazine (DPPHH) spectroscopic probe.","authors":"Mengge Wang, Xiaofeng Chen, Zhigang Wang, Ming Li","doi":"10.1007/s44211-025-00744-6","DOIUrl":"https://doi.org/10.1007/s44211-025-00744-6","url":null,"abstract":"Ionic liquids (ILs) which belong to the molten salt of a high ionic environment exhibit a number of unique properties, including the formation of various heterogeneities in their microemulsion. We found that auto-generated radicals within a [Bmim][PF6]/[C16mim][PF6]/PBS reverse micelles (IL-RMs) and then the radicals were quantified in this study. For the radical confirmation, EPR spectroscopy with 5,5-dimethyl-1-pyrroline N-oxide (DMPO) free radical scavenger was carried out. A spectroscopic chemical probe of 1,1-diphenyl-2-picrylhydrazine (DPPHH) was employed to quantify the radicals in the IL-RM systems. The mechanism of the radical generation was also proposed. The DPPHH probe method is simple, rapid, and sensitive to quantify free radicals in the IL-RM systems. Finally, IL-RMs were successfully applied to degrade Rhodamine B (Rho-B) dye with low degradation cost, simple operation, short time consumption, and remarkable degradation effect. The results show a near 100% degradation rate for 10 μmol·L-1 Rho-B in the IL-RMs system with pH 7.4 PBS containing 1 mmol·L-1 chloroacetic acid as an aqueous phase.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring liquid-liquid phase separation in vitro and in vivo using multimodal nonlinear optical imaging.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-20 DOI: 10.1007/s44211-025-00747-3

Yusuke Murakami, Mia Obuchi, Hiroshi Kamizawa, Shinichi Miyazaki, Akihiro Kishimura, Ryosuke Oketani, Kotaro Hiramatsu, Philippe Leproux, Yu Hayashi, Kentaro Shiraki, Hideaki Kano

引用次数: 0

Electrochemically active DNA ligands for gene detection: present and future.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-03-19 DOI: 10.1007/s44211-025-00745-5

Shigeori Takenaka, Shinobu Sato

{"title":"Electrochemically active DNA ligands for gene detection: present and future.","authors":"Shigeori Takenaka, Shinobu Sato","doi":"10.1007/s44211-025-00745-5","DOIUrl":"https://doi.org/10.1007/s44211-025-00745-5","url":null,"abstract":"Electrochemical gene sensing methods are gaining attention as diagnostic chips. Here, we review the electrochemically active DNA ligand-based sensing methods. Various DNA ligands have been reported in these studies, among which metal complexes, methylene blue, and ferrocenyl naphthalene diimide (FND) have been studied in detail. DNA probe immobilized electrodes have been created, hybridization reactions on the electrodes with target DNA fragments have been performed, and electrochemical gene detection has been possible using these DNA ligands. An example of the realization of this system is the successful and accurate cancer diagnosis using FND to examine abnormal methylation of the hTERT gene, providing reassurance about the system's reliability. In addition, electrochemical detection of PCR products has been realized using the current decrease due to the double-stranded DNA binding of methylene blue although it is a signal-off system. A naphthalene diimide derivative with ferrocene and β-CD, FNC, increased the current upon double-stranded DNA binding. Using these FNCs, the detection of PCR products in a homogeneous system was realized. Electrochemical qPCR was realized with these ligands. Since FNDs also bind strongly to tetraplex or G-quadruplex (G4) DNA, we succeeded in electrochemically detecting telomerase activity, which is known as a cancer marker, using FNDs to detect the amount of telomeric DNA elongation, which is its substrate, as the amount of G4 DNA. This technique has realized compassionate cancer diagnosis from oral swab fluid. It is known that G4 is also present in viral genome RNA, and a viral testing method using G4 is expected to be a potential alternative to PCR. The first example was the electrochemical detection of novel coronaviruses using incFND as an RNA G4 ligand.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0