Analytical Sciences最新文献

{"title":"Biomineralized gold nanoparticle-based colorimetric sensing platform for ascorbic acid detection.","authors":"Zhuqing Wang, Junyuan Zhang, Yuetong Ma, Mengran Zhang, Huixue Zhang, Bing Yang, Qiaorong Tang","doi":"10.1007/s44211-025-00852-3","DOIUrl":"https://doi.org/10.1007/s44211-025-00852-3","url":null,"abstract":"<p><p>Ascorbic acid (AA), a critical regulator of redox homeostasis, plays a vital role in disease prevention. Thus, the development of a rapid and precise detection method is urgently needed. In this work, a colorimetric sensing platform based on bioinspired mineralization-synthesized gold nanoparticles (AuNPs) was proposed for the visual and rapid detection of AA. The as-prepared AuNPs exhibited uniform morphology and excellent oxidase-like activity, efficiently catalyzing 3,3',5,5'-tetramethylbenzidine (TMB) oxidation to produce a characteristic 650 nm absorption peak. However, in the presence of AA, TMB oxidation was significantly inhibited due to the reducing property of AA. The color change was visually detectable within 5 min. The absorbance at 650 nm linearly decreased with the AA concentration in the range of 1-60 μM (R² = 0.999). The detection limit was estimated to 0.27 μM. Besides, the selectivity was excellent against common interfering species. The detection of AA in complex fruit samples was realized, and the recovery rate was 98.5-106.1%. Owing to the simplicity, cost-effectiveness, high sensitivity, and rapid response, this colorimetric sensing strategy provides new insights into the on-site monitoring of antioxidants in biological samples.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solid-phase extraction of ethyleneamines in river water prior to their determination by high-performance liquid chromatography with ultraviolet detection.","authors":"Azusa Yudo, Tomoko Kemmei, Shuji Kodama, Yoshinori Inoue, Shigehiro Kagaya","doi":"10.1007/s44211-025-00850-5","DOIUrl":"https://doi.org/10.1007/s44211-025-00850-5","url":null,"abstract":"<p><p>A method based on solid-phase extraction (SPE) has been developed for the preconcentration of three kinds of ethyleneamines (EAs) [ethylenediamine (EDA), diethylenetriamine (DETA), and triethylenetetramine (TETA)] in river water for their high-performance liquid chromatographic (HPLC) determination by using on-line complexation with Cu(II) ion and ultraviolet (UV) detection. EAs, which are protonated in acidic and neutral conditions, could be concentrated by using cation-exchange SPE. However, inorganic cations and humic substances, often present in river water, interfered with this preconcentration. Inorganic cations, such as Ca and Mg ions, compete with EAs in cation-exchange processes. This interference could be reduced by masking them with ethylenediaminetetraacetic acid. Humic substances, such as humic acid and fluvic acid, seem to be due to the electrostatic interaction of EAs with them in the sample solution. This interference was reduced by passing the sample solution through an anion-exchange cartridge before the cation-exchange SPE. The interacted EAs remained in the anion-exchange cartridge; they could be eluted by washing out the anion-exchange cartridge with diluted HCl. In this washing process, although fulvic acid was eluted at the same time, it could be removed by passing the eluate through a reversed-phase cartridge connected in a series. After removing these interferences, EAs could be extraced with the cation-exchange SPE and quantitatively eluted by passing Cu(II) solution through the cartridge. The proposed method of including the HPLC-UV combined with the SPE was available for the determination of EAs in river water at concentrations as low as 0.015 µM for EDA and DETA and 0.05 µM for TETA. The high recoveries (83%-103%) and repeatabilities (RSD 1.2%-4.3%) were obtained for EAs from two river water samples spiked with two different concentrations of EAs. The proposed method was applied to analyses of river water samples taken from four rivers in Toyama Prefecture, Japan.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virus inactivation effect of hypochlorite solution containing nitrate ions.","authors":"Erina Satake, Maho Ishiyama, Masaki Sekiguchi, Atsushi Sasaki, Shigekazu Yano, Masatoshi Endo","doi":"10.1007/s44211-025-00831-8","DOIUrl":"https://doi.org/10.1007/s44211-025-00831-8","url":null,"abstract":"<p><p>Hypochlorous acid (HClO) is widely used as a disinfectant due to its strong oxidizing power, but its efficacy is highly dependent on pH and decreases under neutral to alkaline conditions. In addition, its high concentration poses safety and environmental concerns. In this study, we investigated the enhancement of virucidal activity at lower HClO concentrations through the formation of monochloramine (NH₂Cl) by supplementing with ammonium-form nitrogen (NH₄⁺-N) or nitrate-form nitrogen (NO₃^--N) under different pH conditions. Using bacteriophage φ6 as an enveloped virus model, plaque assays revealed that virus inactivation was most effective at pH 8 when 15-20 ppm of NH₄⁺-N was added to 30 ppm HClO solution, with over 90% reduction in infectivity. Under acidic conditions (pH 6), the formation of NH₂Cl was limited due to the rapid consumption of free chlorine and insufficient generation of NH₃, resulting in reduced virucidal efficacy; however, increasing the amount of NH₄⁺-N led to improved inactivation effects. In contrast, at pH 8, NH₂Cl formation was enhanced, contributing to improved disinfection efficiency. Similar effects were observed when NO₃^--N was used, likely due to its reduction to NH₄⁺ in the presence of organic matter. These results demonstrate that optimizing pH and nitrogen sources can improve the efficacy of low-concentration HClO solutions by generating NH₂Cl in situ, offering a safer and more sustainable approach to viral disinfection.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in microfluidic chip technology for cell analysis.","authors":"Luxi Shu, Yan Zhang","doi":"10.1007/s44211-025-00851-4","DOIUrl":"https://doi.org/10.1007/s44211-025-00851-4","url":null,"abstract":"<p><p>Microfluidic chips play a crucial role in the field of cell analysis. These miniature chips integrate seamlessly across key cell analysis stages, including sample preparation, cell culture, sorting, lysis, and detection. They possess many advantages such as miniaturization, integration, automation, and portability. The size of their microchannels is comparable to that of cells, thus enabling research to be conducted at the single-cell or even subcellular organelle level. They can also simulate the physiological conditions in vivo, conduct non-destructive or minimally destructive detections, and meet the requirements of high-throughput cell analysis, which is beneficial for parallel operations and continuous analysis. This article reviews the technological progress of microfluidic chips, focusing on three major directions: cell sorting and enrichment, single-cell analysis, and dynamic microenvironment simulation. The article also analyzes the challenges faced by this technology, such as cell damage control, handling of multicellular heterogeneity, data interpretation, etc., and proposes coping strategies such as the development of new biomaterials, multimodal integration technology, and artificial intelligence assistance. By combining cutting-edge technologies such as nanotechnology, 3D printing, and organoid culture, the functions of microfluidic chips can be further expanded to enable the simulation and analysis of more complex biological systems and provide important technical support for cell biology research and clinical translation.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent topics in X-ray absorption spectroscopy: integrating theory, instrumentation, and applications","authors":"Daitaro Ishikawa","doi":"10.1007/s44211-025-00840-7","DOIUrl":"10.1007/s44211-025-00840-7","url":null,"abstract":"","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 10","pages":"1573 - 1574"},"PeriodicalIF":2.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145135100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rolling circle transcription-based fluorescent aptasensor for highly sensitive and onsite detection of microcystin-LR.","authors":"Shuran Yang, Yuling Xu, Cheng Zhang, Ke Xu, Zhiyong Hu, Zhiqiang Xia","doi":"10.1007/s44211-025-00839-0","DOIUrl":"https://doi.org/10.1007/s44211-025-00839-0","url":null,"abstract":"<p><p>Onsite and sensitive detection of microcystin-LR (MC-LR) is crucial for monitoring environmental pollution and protecting human health, particularly in the context of increasing cyanobacterial harmful algal blooms (CyanoHABs). Herein, this study reports a rolling circle transcription (RCT)-based fluorescent aptasensor designed for rapid and quantitative onsite detection of MC-LR in real water samples. Aptamers specific to MC-LR were immobilized onto magnetic beads (MBs) and hybridized with blocker complementary DNA strands to form the MB aptasensor complex. Upon recognition and binding of MC-LR molecules in water samples, the blocker DNA strands were displaced and subsequently cyclized into circular DNA structures via T4 DNA ligase-mediated ligation. These circular DNA templates initiated RCT, generating abundant fluorescent Mango RNA reporters. The resulting fluorescent signals exhibited a robust positive correlation with MC-LR concentration, with a detection limit of 39 pM and a linear range from 0.01 to 3000 nM, demonstrating high sensitivity and specificity. The aptasensor showed excellent specificity against structural analogs and common interferents. Recovery tests in Haihe River samples yielded rates between 98.33% and 101.17%, with relative standard deviations below 5%. This sensing platform offers a simple operational procedure, minimal sample preparation, and excellent applicability for real environmental water monitoring. Thus, the developed RCT-based aptasensor provides a promising, cost-effective alternative for onsite MC-LR detection and contributes significantly to environmental safety and public health management.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new approach for determining trace platinum concentrations in macroalgae and evaluating tissue-specific distribution.","authors":"Ziwei Li, Asami S Mashio, Kuo H Wong, Hiroshi Hasegawa","doi":"10.1007/s44211-025-00849-y","DOIUrl":"https://doi.org/10.1007/s44211-025-00849-y","url":null,"abstract":"<p><p>Platinum (Pt), extensively used in automotive catalytic converters and anti-cancer drugs, has led to elevated concentrations in the environment. Monitoring Pt levels in coastal ecosystems is crucial as excessive accumulation may cause ecotoxicological impacts. In this study, Undaria pinnatifida, a common edible macroalgae in Japan known for its high affinity for platinum group elements, was evaluated as a biomonitor for monitoring Pt concentrations and assessing its anthropogenic influences in coastal areas. An analytical protocol using inductively coupled plasma mass spectrometry (ICP-MS) was developed and optimized for Pt quantification in macroalgal tissues. The optimal digestion conditions included 0.24 mL/g of hydrofluoric acid and the addition of 7 mL/g of hydrochloric acid, which effectively reduced white precipitate formation and improved Pt recovery. The established method demonstrated excellent sensitivity, achieving a method blank of 16 ppt and a detection limit of 5 ppt, surpassing previously reported approaches. Applying this optimized protocol, tissue-specific Pt concentrations were determined in different parts of U. pinnatifida collected from various coastal zones. The results revealed significant spatial variations in Pt concentrations among plant organs: concentrations in blades, stipes, and sporophylls decreased from the outer bay to the inner bay, while holdfasts concentrations exhibited the opposite trend. These findings suggest distinct Pt accumulation behaviors between above-ground and below-ground tissues of U. pinnatifida, underscoring its potential for monitoring spatial variations of Pt in coastal environments.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Statistical quantification of SERS signals in microfluidic flow using AuNP-bound polystyrene microparticles.","authors":"Shu Mingcong, Akihisa Miyagawa, Shoji Ishizaka","doi":"10.1007/s44211-025-00846-1","DOIUrl":"https://doi.org/10.1007/s44211-025-00846-1","url":null,"abstract":"<p><p>Surface-enhanced Raman scattering (SERS) is a powerful analytical technique; however, its quantitative application has been limited by the instability of substrates and significant signal fluctuations. In this study, we demonstrated that 4-aminobenzenethiol (4-ATP) can be quantitatively detected through statistical analysis of SERS signal intensity distributions obtained using citrate-stabilized AuNPs, biotin-functionalized AuNPs, and gold nanoparticle (AuNP)-bound polystyrene (PS) microparticles. Raman spectra obtained in bulk aqueous solution under static conditions showed that the detection sensitivity of 4-ATP using AuNP-bound PS microparticles was approximately twice that achieved with citrate-stabilized AuNPs or biotin-modified AuNPs. Furthermore, the detection sensitivity of 4-ATP was enhanced by introducing AuNP-bound PS microparticles into a microfluidic chip and delivering an aqueous 4-ATP solution under controlled flow conditions. Analysis of the flow rate dependence of SERS signal intensity revealed that the optimal detection sensitivity for 4-ATP was achieved at a flow rate of 0.66 μL·min⁻¹, with a corresponding detection limit of 1.9 μM under these conditions. These results demonstrate that AuNP-bound PS microparticles introduced into the microfluidic chip serve as efficient SERS substrates, enabling highly sensitive and quantitative detection under flow conditions.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization and application of pretreatment for the analysis of typical per- and polyfluoroalkyl substances (PFAAs) in drinking water: a systematic evaluation of filter membranes and SPE Sorbents.","authors":"Duwang Li, Wanyuan He, Lingling Liu, Wanlu Ren, Yiqi Wan, Huiping Deng, Hongwei Yang","doi":"10.1007/s44211-025-00847-0","DOIUrl":"https://doi.org/10.1007/s44211-025-00847-0","url":null,"abstract":"<p><p>The accurate detection of trace perfluoroalkyl acids (PFAAs) in drinking water remains challenging due to nonspecific adsorption losses during pretreatment. This study systematically evaluated the adsorption behaviors of 11 PFAAs across five filtration membranes and four solid-phase extraction (SPE) sorbents to establish an optimized analytical protocol. Results demonstrated that glass fiber (GL) filters minimized PFAAs retention (94.85% recovery in aqueous matrices) through electrostatic repulsion, outperforming other membranes such as polypropylene(PP). Among SPE sorbents, Weak Anion Exchange (WAX) exhibited the highest recovery rates among the sorbents evaluated (100.04% ± 2.74% recovery) via a dual adsorption mechanism combining amine-mediated ion exchange and hydrophobic interactions, achieving 30% higher efficiency than Hydrophile-Lipophile Balance (HLB) sorbents. The optimized method achieved low detection limits (LOD: 0.016-0.324 ng/L), high accuracy (spiked recovery: 80.12-115.76%) and precision (RSD < 10%). Method application revealed significantly lower PFAAs contamination in reservoir-sourced finished water compared to river/lake-derived supplies in the mid-Yangtze River region. This work effectively mitigates critical pretreatment biases in PFAAs analysis and provides a reliable solution to address the urgent need for monitoring PFAAs contamination in drinking water systems.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of phenylphosphonic acid zinc salt in poly(lactic acid) using thermally assisted methylation pyrolysis-gas chromatography/mass spectrometry.","authors":"Kazuko Matsui, Makoto Niwa, Atsushi Watanabe, Chuichi Watanabe, Norio Teramae","doi":"10.1007/s44211-025-00845-2","DOIUrl":"https://doi.org/10.1007/s44211-025-00845-2","url":null,"abstract":"<p><p>Biomass-based polymers such as poly(lactic acid) (PLA) have attracted much attention, because they are renewable, biocompatible, and nontoxic to the environment and have been used in various fields such as biomedical, agricultural, and food packaging industries. However, one of the common drawbacks of PLA-based materials is their low glass transition temperature in the amorphous state, while adding phenylphosphonic acid zinc salt (PPA-Zn) as a nucleating agent was found to be a promising method to improve the physical property of PLA. On the other hand, degradation of PLA-based materials in the environment may cause the pollution from the metal of a nucleating agent in PLA and quantification of nucleating agents in polymers is of interest. Polar phosphonic acid, a ligand in PPA-Zn, is not favorable for the analysis by gas chromatography/mass spectrometry (GC/MS). Such compounds containing polar groups are known to be effectively analyzed by GC/MS in combination with thermally assisted methylation reaction. In this study, we report on determination of PPA-Zn in PLA by coupling thermally assisted methylation with pyrolysis-GC/MS.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}