Analytical Sciences最新文献

{"title":"Light-up RNA aptamer-based T-NASBA: a one-pot, label-free strategy for miRNA detection","authors":"Yao Fu,&nbsp;Xuejuan Pei,&nbsp;Junhua Cao,&nbsp;Qiang Chen,&nbsp;Shixiong Deng","doi":"10.1007/s44211-025-00811-y","DOIUrl":"10.1007/s44211-025-00811-y","url":null,"abstract":"<div><p>MicroRNAs (miRNAs) are pivotal regulators of cellular processes, with dysregulation linked to diverse diseases, particularly cancer. Current methods, while effective, are often expensive, complex, and require sophisticated equipment. To address these limitations, we developed a label-free, one-pot miRNA detection platform integrating toehold-mediated strand displacement (TMSD) with nucleic acid sequence-based amplification (NASBA). This method utilizes a TMSD-regulated hairpin Primer1 that selectively responds to target miRNA, triggering NASBA to generate a light-Up RNA aptamer for real-time, target-dependent fluorescence signaling-eliminating the need for fluorophore-labeled probes or additional modifications. By systematic optimization of the reaction conditions, the platform achieved a detection limit of 4.31 pM, with a strong linear correlation between fluorescence intensity and miRNA concentration over a range from 10 pM to 1 nM. The platform also demonstrated excellent reproducibility and precision in spike-and-recovery tests with human serum, with recovery rates between 99.85<span>(%)</span> and 108.28<span>(%)</span>. T-NASBA provides a novel, cost-effective, and label-free platform for miRNA detection, offering a simple alternative that requires no complex instrumentation. This makes T-NASBA ideal for a wide range of applications, including point-of-care diagnostics and research, further advancing the field of miRNA-based diagnostics and monitoring.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 10","pages":"1607 - 1616"},"PeriodicalIF":2.0,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A conjugated pyridine diimine fluorene-based polymer for the detection of furaltadone","authors":"Lindan Gong,&nbsp;Yan Sun,&nbsp;Hongju Zhang,&nbsp;Junqing Li,&nbsp;Aohui Qin,&nbsp;Lu-an Fan","doi":"10.1007/s44211-025-00829-2","DOIUrl":"10.1007/s44211-025-00829-2","url":null,"abstract":"<div><p>The potential risks posed by antibiotics to human health and the ecological environment have long been a major public concern. Consequently, developing probe technologies capable of efficiently detecting trace levels of antibiotics in environments has emerged as a critical research priority. In this study, a conjugated fluorene-based pyridine diimine polymeric probe (FPD) was employed as a fluorescent probe for the specific recognition and detection of furaltadone. Experimental results showed that FPD rapidly interacts with furaltadone, resulting in a significant fluorescence quenching of FPD, and its fluorescence intensity could quickly reach a stable state. In the ethanol-PBS mixed solution system, the fluorescence quenching efficiency of FPD exhibits an excellent linear relationship with furaltadone concentrations ranging from 0 to 12 µM, achieving a detection limit as low as 0.0490 µM. In the presence of interfering substances, FPD maintains superior selectivity and anti-interference capability. Furthermore, a recovery experiment was conducted by spiking furaltadone into egg white, yielding recovery rates ranging from 97.29% to 104.22%, with a relative standard deviation (RSD) of less than 3.73%. This study develops a novel and reliable method for the efficient and precise detection of furaltadone, demonstrating broad application potential.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 10","pages":"1659 - 1668"},"PeriodicalIF":2.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surfactant aggregates for preconcentration of trace analytes","authors":"Hiroaki Matsumiya","doi":"10.1007/s44211-025-00810-z","DOIUrl":"10.1007/s44211-025-00810-z","url":null,"abstract":"","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 8","pages":"1103 - 1105"},"PeriodicalIF":2.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous determination of mumefural and citric acid in Japanese apricot fruit juice concentrate using LC with intramolecular excimer-forming derivatization","authors":"Hideyuki Yoshida,&nbsp;Yuka Imura,&nbsp;Sayaka Minematsu,&nbsp;Misaki Ono,&nbsp;Reiko Koga,&nbsp;Hitoshi Nohta","doi":"10.1007/s44211-025-00822-9","DOIUrl":"10.1007/s44211-025-00822-9","url":null,"abstract":"<div><p>Mumefural (MF) which is one of the citrate esters with 5-hydoxymethyl-2-furfural is a component of Japanese apricot fruit juice concentrate (plum extract). A selective LC-fluorescence detection method was developed for the simultaneous quantification of MF and citric acid in plum extracts using intramolecular excimer-forming derivatization. Analytes were derivatized to their corresponding polypyrene derivatives using 4-(1-pyrene)butyric hydrazide (PBH) in the presence of condensation reagent, separated by reversed-phase LC, and detected in the pyrene-excimer fluorescence region (Ex/Em, 345/475 nm). The detection limits (<i>S</i>/<i>N</i> = 3) for the analytes were sub-picomole levels per 10-μL injection volume. The method was applied for the determination of MF and citric acid in plum extracts and a typical plum wine with a simple pretreatment. Large amounts of citric acid were detected in the plum extracts and the plum wine, whereas MF was detected only in the commercial plum extracts.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 9","pages":"1547 - 1553"},"PeriodicalIF":2.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct visualization of phase-separation multiphase flow in a silica bead-packed microchannel using fluorescence microscopy","authors":"Yuya Yamashiro,&nbsp;Takeshi Iharada,&nbsp;Kazuhiko Tsukagoshi","doi":"10.1007/s44211-025-00830-9","DOIUrl":"10.1007/s44211-025-00830-9","url":null,"abstract":"<div><p>We previously developed a high-performance liquid chromatography (HPLC) system employing phase-separation multiphase flow (PSMF) as the eluent, referred to as the phase-separation mode in HPLC. However, direct visualization of the flow behavior within the HPLC column had not yet been achieved. In this study, we directly visualized the PSMF behavior in a silica bead-packed microchannel using fluorescence microscopy. A two-phase separation mixed solution composed of 1-butyl-3-methylimidazolium chloride [(C₄mim)Cl, an ionic liquid], K₂HPO₄, and water, with Eosin Y as a fluorescent dye, was introduced into a microchannel (200 μm wide, 40 μm deep) packed with 10 μm silica beads. Phase separation was induced by cooling from 40 to 25 °C, resulting in an ionic liquid-rich phase containing Eosin Y and a K₂HPO₄-rich phase. Fluorescence microscopy equipped with a CMOS color camera enabled visualization of the flow distribution. When the ionic liquid-rich solution was used, non-fluorescent (black) circular regions approximately 10 μm in diameter—corresponding to the silica beads—were observed, surrounded by green fluorescent areas resulting from the distribution of Eosin Y in the ionic liquid phase. These observations indicate that the ionic liquid-rich phase predominantly flows away from the surfaces of the beads. Conversely, when the K₂HPO₄-rich solution was used, green fluorescent regions appeared at the bead locations, surrounded by non-fluorescent areas. This indicates that the ionic liquid-rich phase, which contains Eosin Y, preferentially flows near the surfaces of the silica beads. These results provide the first direct visual evidence of PSMF behavior within a particle-packed microchannel. The findings support the proposed flow dynamics of PSMF in HPLC columns and validate the separation mechanism of the phase-separation mode.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 10","pages":"1689 - 1694"},"PeriodicalIF":2.0,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-antibody sandwich immunoassay using upconversion nanoparticles and magnetic beads for fibrinogen detection","authors":"Ke Lv,&nbsp;Weitao Wang,&nbsp;Li Zhang,&nbsp;Xi Zhou,&nbsp;Hongyan Yu,&nbsp;Xiaole Han,&nbsp;Li Wang,&nbsp;Guoming Xie,&nbsp;Gang Yang","doi":"10.1007/s44211-025-00826-5","DOIUrl":"10.1007/s44211-025-00826-5","url":null,"abstract":"<div><p>Fibrinogen is a vital biomarker for coagulation disorders, with reduced levels increasing bleeding risk and mortality, requiring rapid detection for early diagnosis. Enzyme-linked immunosorbent assay (ELISA) is commonly employed in clinical environments; however, its prolonged processing duration and insufficient sensitivity hinder its utility for swift diagnostics. We addressed these limitations by creating a dual-antibody sandwich immunoassay that employs upconversion nanoparticles (UCNPs) and magnetic beads (MBs) for the precise and sensitive detection of fibrinogen. The enhanced analytical performance of the method is attributed to the use of UCNPs and MBs. UCNPs enhance the signal-to-noise ratio by emitting visible light under near-infrared excitation, which reduces background interference from autofluorescence and light scattering in biological samples. Meanwhile, MBs facilitate specific enrichment of fibrinogen by selectively capturing the target analyte, enabling efficient isolation from complex sample matrices. This synergy improves sensitivity and specificity, achieving a 2 ng/mL detection limit with a 15-fold sensitivity increase, simpler operation, and shorter detection time compared to conventional ELISA kits. This platform offers a robust solution for fibrinogen detection, with potential for broad application in clinical diagnostics.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 10","pages":"1647 - 1657"},"PeriodicalIF":2.0,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a sensor-compatible vascular microphysiological system for metabolic monitoring during drug-induced endothelial injury","authors":"Yuning Fu,&nbsp;Ryota Okitsu,&nbsp;Yuji Nashimoto,&nbsp;Yasuhiko Shinoda,&nbsp;Yoshinobu Utagawa,&nbsp;Masateru Yamazaki,&nbsp;Koki Nakaya,&nbsp;Kosuke Ino,&nbsp;Hirokazu Kaji","doi":"10.1007/s44211-025-00825-6","DOIUrl":"10.1007/s44211-025-00825-6","url":null,"abstract":"<div><p>Endothelial metabolism is closely linked to vascular function and is often altered in response to pathological stimuli. Although vascular microphysiological systems (MPS) offer a promising in vitro platform for modeling vascular environments, the integration of real-time metabolic monitoring remains technically challenging. Enzyme-based electrochemical sensors are well-suited for detecting metabolites, such as glucose and lactate; however, their direct incorporation into culture systems is limited by the inactivation of enzymes under culture conditions. In this study, we investigated a vascular MPS with a sensor-compatible design to support future integration of printed enzyme-based biosensors. The device features a stacked open-bottom architecture that enables the integration of biosensors beneath the endothelial layer after monolayer formation, thus minimizing sensor exposure during culture. We validated the formation of an endothelial monolayer on a porous polyurethane membrane with a mortar-like structure and confirmed its compatibility with a model sensor substrate. As a preliminary step toward sensor-based metabolic analysis, we quantified glucose consumption and lactate production during endothelial monolayer formation and upon exposure to Minoxidil and Hydralazine, drugs known to induce vascular injury. These findings demonstrate the feasibility of sensor-compatible vascular MPS and provide foundational data to support the future development of integrated platforms for real-time metabolic monitoring in drug toxicity studies.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 10","pages":"1617 - 1625"},"PeriodicalIF":2.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cascade signal amplification strategy for the ultrasensitive fluorescence detection of kanamycin base on exonuclease III and mismatched catalytic hairpin assembly","authors":"Zhen Liu,&nbsp;Xing Liu,&nbsp;Qian Wu,&nbsp;Xilin Xiao","doi":"10.1007/s44211-025-00816-7","DOIUrl":"10.1007/s44211-025-00816-7","url":null,"abstract":"<div><p>This study employs a guanine (G)-rich DNA sequence as the recognition element and integrates exonuclease III (Exo III) with a mismatch catalytic hairpin assembly (MCHA)-based cascade isothermal signal amplification strategy to construct a novel fluorescent DNA biosensor for the highly sensitive detection of kanamycin (Kana). In the presence of the target, the recognition element HP1 is unfolded by the target and forms a double-stranded HP1-HP2 structure with HP2. This structure is subsequently cleaved by Exo III, releasing the trigger strand of MCHA. The trigger strand binds to H1, which contains dual fluorescent groups, resulting in the separation of carboxyfluorescein (FAM) and tetramethylrhodamine (TAMRA). This separation attenuates fluorescence resonance energy transfer and restores FAM fluorescence, generating a strong fluorescence signal at 520 nm. The fluorescence sensor demonstrates a linear detection range from 2 to 12 nM, with a detection limit of 0.16 nM. In real milk samples, the spiked recovery rate ranges from 97.4 to 106.3%, with relative standard deviations between 2.2 and 3.8%. The cascade isothermal signal amplification strategy significantly enhances the sensor's sensitivity, while MCHA reduces false positive rates. This aptamer-based sensor exhibits excellent specificity, minimal susceptibility to interference, and suitability for detecting Kana in milk.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 9","pages":"1523 - 1530"},"PeriodicalIF":2.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An ultrasensitive sensing strategy based on CRISPR/Cas13a and T7 RNA polymerase amplification for detection of extracellular vesicles","authors":"Fengying Ran,&nbsp;Huimin Huang,&nbsp;Bing Shang,&nbsp;Weidong Peng,&nbsp;Lun Wu,&nbsp;Kang Ling,&nbsp;Xiaoyu Xie","doi":"10.1007/s44211-025-00828-3","DOIUrl":"10.1007/s44211-025-00828-3","url":null,"abstract":"<div><p>Extracellular vesicles (EVs) are important biomarkers for an early diagnosis of lung cancer. Herein, we proposed an ultrasensitive fluorescent sensing platform for EVs detection, which involves aptamer and streptavidin-modified magnetic nanoparticles (SA-MB) magnetic separation technology as well as T7 RNA polymerase-assisted CRISPR/Cas13a system, which can achieve target recycling signal amplification. In this detection method, biotin-modified CD63 aptamer hybridizes first with the aptamer Blocker (T7 promoter) and then binds to SA-MB. When adding EVs, the CD63 aptamer in CD63 aptamer/Blocker/SA-MB complex captures EVs causing the release of Blocker single chain. Subsequently, large amounts of ssRNAs, which are generated with the assistance of Blocker-initiated T7 RNA polymerase, were recognized by CRISPR/Cas13a and trigger its trans-cleavage report probe (F-Q). Eventually, the report probe labeled with fluorescent dye (FAM) and quench group (BHQ) at both ends was cut to produce fluorescent signal. The designed sensor combined this with a signal amplification strategy based on T7 RNA polymerase and CRISPR/Cas13a to significantly enhance the sensitivity and specificity of EVs detection. The use of magnetic separation technology eliminates interference from complex matrices and improves EVs detection efficiency, while the introduction of T7 RNA polymerase and CRISPR/Cas13a enables multiple amplifications of the sensor signals, and enhancing the accuracy and sensitivity of the method. Ultimately, the combination of multiple amplification reactions resulted in a detection limit (LOD) for EVs as low as 60 particles/mL (approximately 1 zmol/L). In addition, this detection method can specifically distinguish EVs from other confounding substances and efficiently detect plasma EVs from lung cancer and healthy individuals in actual samples. Indicating this sensing platform is a valuable tool for early lung cancer detection.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 10","pages":"1627 - 1636"},"PeriodicalIF":2.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-025-00828-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress and prospect: major metabolite analysis of central carbon metabolism with saccharification using mix-mode chromatography-application to multiple parallel fermentation of sake","authors":"Daisuke Kozaki","doi":"10.1007/s44211-025-00819-4","DOIUrl":"10.1007/s44211-025-00819-4","url":null,"abstract":"<div><p>Japanese sake breweries, most of which generally operate as small- and medium-sized companies, use the central carbon metabolism (comprising glycolysis, the tricarboxylic acid cycle, and ethanol fermentation) for producing sake. However, it is difficult for such companies to use expensive advanced instrumentation. Metabolite analysis using LC–MS or GC–MS could be more useful for such breweries. Our research group has developed various mix-mode chromatography (MMC) methods for analyzing the major metabolites produced during the multiple parallel fermentation (MPF) of sake. This mini review reports on three different MMCs and makes an attempt to understand the behaviors of major metabolites of central carbon metabolism, along with saccharification, during the MPF of sake.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 8","pages":"1145 - 1155"},"PeriodicalIF":2.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}