Carbon black imprinted o-diaminobenzene sensing platform for sensitive electrochemical detection of interleukin 6

IF 1.8 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences Pub Date : 2025-02-21 DOI:10.1007/s44211-024-00709-1

Xu Cui, Zhiqiang Chen, Longyao Xu, Changwei Wu, Bin Qiu, Chaoming Zhou

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引用次数: 0

Abstract

As a typical proinflammatory cytokine, interleukin 6 (IL-6) exhibits great influence on treating diseases (e.g., urocystitis) and adjusting immune. Normally, the IL-6 degree keeps a low content (< 40.0 pg mL⁻¹) in bodies, but when its degree becomes higher, several serious conditions (e.g., urocystitis and infections) may occur. Hence, it is very essential to design a highly effective technology for monitoring IL-6 as a biomarker. In the present work, through adopting carbon black (CB) as substrate substance, o-diaminobenzene as electropolymerized monomer and [Fe(CN)₆]^4−/3− as the signal probe, a low-cost, sensitive and simple molecularly imprinted polymer (MIP) electrochemical sensing platform was designed to detect the IL-6 degree. The experiments revealed that the current of [Fe(CN)₆]^4−/3− probe at the MIP sensor would decrease along with the increase of IL-6 degree. Owing to the prominent properties of CB (e.g., high conductivity and surface area as well as stability) and high target-recognition capability of MIP, the as-proposed MIP sensor offers wide linearity (0.1–800 pg mL⁻¹) and low analytical limit (0.03 pg mL⁻¹); meanwhile, it possesses good repeatability, stability, and practical applications. It is, thus, expected this simple MIP sensor has some application value for IL-6 detection.

Graphical abstract

查看原文本刊更多论文

炭黑印迹邻二氨基苯敏感电化学检测白细胞介素6的平台。

白细胞介素6 （IL-6）是一种典型的促炎细胞因子，在治疗疾病（如尿囊炎）和调节免疫等方面具有重要作用。正常情况下，IL-6在体内保持较低的含量（-1），但当IL-6升高时，可能出现一些严重的情况（如尿膀胱炎和感染）。因此，设计一种高效的IL-6生物标志物监测技术是非常必要的。本文以炭黑（CB）为底物，邻二氨基苯为电聚合单体，[Fe(CN)6]4-/3-为信号探针，设计了一种低成本、灵敏、简单的分子印迹聚合物（MIP）电化学传感平台，用于检测IL-6度。实验表明，随着IL-6度的增加，MIP传感器处的[Fe(CN)6]4-/3-探针电流减小。由于CB的突出特性（例如，高导电性和表面积以及稳定性）和MIP的高目标识别能力，所提出的MIP传感器具有宽线性（0.1-800 pg mL-1）和低分析限（0.03 pg mL-1）；同时具有良好的重复性、稳定性和实际应用价值。因此，期望这种简单的MIP传感器对IL-6的检测具有一定的应用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical Sciences 化学-分析化学

CiteScore

2.90

自引率

18.80%

发文量

232

审稿时长

1 months

期刊介绍： Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.