Determination of circulating tumor cells by surface-enhanced Raman scattering based on molecularly imprinted polymers doped with silver nanoparticles

IF 1.8 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences Pub Date : 2025-02-25 DOI:10.1007/s44211-025-00719-7

JunJie Wei, Yong Shen, GangFeng Ouyang

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Abstract

The rapid and simple detection of circulating tumor cells (CTCs) can help with early diagnosis of tumor diseases and assist clinical judgment of tumor disease development status and prognosis. An important alteration in cancer progression is a significant increase in sialic acid (SA) levels, making sialic acid one of an interesting biomarker for cancer type diversity. Here, novel SERS nano-biosensors were fabricated employing molecularly imprinted polymers (MIPs) particles loaded with Ag nanoparticles (AgNPs) to form high affinity toward sialic acid on CTCs. In this work, the glycol-specific MIPs are produced using precipitation polymerization. The nanocomposite was characterized by Fourier transform infrared spectroscopy (FTIR), zeta potential, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Consequently, CTC can be rapidly detected in mouse blood in a specific and sensitive manner, as low as 1 cells/ml. In addition, a good linear relationship was observed between CTC concentration and signal intensity (R² = 0.9881). Moreover, the SERS nano-biosensors was used to detect CTC with excellent precision (relative standard deviation, RSD = 3.9%) and good repeatability (RSD = 6.8%). Therefore, this inexpensive, highly sensitive, and rapid method using SERS nano-biosensors can quickly detect CTCs and may prove to be a new platform to be an early diagnostic tool for cancer patient detection.

Graphical abstract

Abstract Image

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循环肿瘤细胞（CTCs）快速、简便的检测有助于肿瘤疾病的早期诊断，有助于临床判断肿瘤疾病的发展状况和预后。唾液酸（SA）水平的显著增加是癌症进展中的一个重要变化，这使得唾液酸成为癌症类型多样性的一个有趣的生物标志物。本文利用分子印迹聚合物（MIPs）颗粒装载银纳米颗粒（AgNPs），在ctc上形成对唾液酸的高亲和力，制备了新型SERS纳米生物传感器。在这项工作中，用沉淀聚合法制备了乙醇特异性的mip。采用傅里叶变换红外光谱（FTIR）、zeta电位、扫描电镜（SEM）和透射电镜（TEM）对纳米复合材料进行了表征。因此，CTC可在小鼠血液中快速、特异、灵敏地检测到，低至1个细胞/ml。CTC浓度与信号强度呈良好的线性关系（R2 = 0.9881）。SERS纳米生物传感器检测CTC具有良好的精密度（相对标准偏差，RSD = 3.9%）和重复性（RSD = 6.8%）。因此，这种使用SERS纳米生物传感器的廉价、高灵敏度和快速的方法可以快速检测ctc，并可能被证明是一个新的平台，成为癌症患者检测的早期诊断工具。

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来源期刊

Analytical Sciences 化学-分析化学

CiteScore

2.90

自引率

18.80%

发文量

232

审稿时长

1 months

期刊介绍： Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.