Analytical Sciences最新文献

{"title":"Simple, accurate, and precise detection of total nitrogen in surface waters using a smartphone-based analytical method.","authors":"Fatih Polat","doi":"10.1007/s44211-024-00696-3","DOIUrl":"https://doi.org/10.1007/s44211-024-00696-3","url":null,"abstract":"<p><p>In this study, a simple, accurate, and precise analytical strategy was developed for the determination of total nitrogen (total dissolved nitrogen + particulate nitrogen) in surface waters using smartphone technology. The spectrophotometric method based on digestion with persulfate, commonly used in water analyses, was integrated with a digital image-based colorimetric detection system (DIC). To evaluate the accuracy of the total nitrogen-digital image-based colorimetric detection system (TN-DIC), results obtained from the Hach method and UV-VIS spectrophotometer were compared with the RGB values obtained from the smartphone imaging system. T-Test results indicated no statistically significant difference between the results obtained from the TN-DIC method and the standard methods. The linear range of the TN-DIC method was determined to be 0.25-5 mg/L. The analytical quality indicators of the system were established as follows: limit of detection (LOD) 0.095 ppm, limit of quantification (LOQ) 0.29 ppm, %RSD 0.32, and R² value 0.9982. Recovery percentages (99-112%) used to measure the analytical performance of the method were calculated through spike applications at concentrations of 1 and 2 ppm in real water samples. Unlike traditional spectrophotometric methods, this smartphone-based approach is fast, simple, and cost-effective, requiring no complex equipment during analysis.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality control of corn silk extract using IR spectroscopy along with statistical methods.","authors":"Aiko Sano, Yutaka Inoue, Yuji Higuchi, Ken-Ichi Akao, Ryuichiro Suzuki","doi":"10.1007/s44211-024-00699-0","DOIUrl":"https://doi.org/10.1007/s44211-024-00699-0","url":null,"abstract":"<p><p>Aqueous extracts of corn silk exhibit glycation-inhibitory activity. Lignin is the active component of these extracts. As corn silk is highly nutritious and has medicinal value, it can be used as a functional food and cosmetics. However, to achieve this goal, it is necessary to evaluate its quality. As lignin, which could be used as a marker compound for quality control, is a macromolecule, HPLC cannot be employed for the quality control of the aqueous extracts of corn silk. We here develop a method to evaluate the anti-glycation activity of the aqueous extracts of corn silk using attenuated total reflectance (ATR)-Fourier transform infrared (FTIR) spectroscopy along with multivariate statistical analysis. The inhibitory activity was evaluated using two multivariate calibrations: principal component regression (PCR) and partial least square regression (PLSR). The spectral areas of the PCR model were 633.5-880.3, 1191.8-1359.6, 1423.1-1492.6, and 2572.6-2974.7 cm^-1. Its coefficient of correlation (R² = 0.981) and root mean square error of cross validation (RMSECV = 2.356) were highly predictable. The spectral regions of 983.5-985.5 and 1021.1-1107.9 cm^-1 offered the best prediction models for the PLSR model. The R² value for the correlation between the actual values and the FTIR-predicted values was 0.994, while the corresponding RMSECV was 1.325%. Hence, FTIR spectroscopy along with multivariate calibration is a useful method for evaluating active corn silk aqueous extracts.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid purification method for trace amounts of cell-free DNA in urine.","authors":"Ryo Fukushima, Yuina Ogura, Chikako Hosokawa, Noriko Watanabe, Fumihiro Ishikawa, Motoko Shibanuma, Masaru Kato","doi":"10.1007/s44211-024-00687-4","DOIUrl":"https://doi.org/10.1007/s44211-024-00687-4","url":null,"abstract":"<p><p>Cell-free DNA (cfDNA) is a valuable biomarker for the early detection of genetic diseases and for evaluating treatment efficacy. We developed a rapid and cost-effective purification method for urinary cfDNA using a commercially available DNA purification kit. This method enables the rapid purification (< 20 min) of DNA suitable for use in the polymerase chain reaction (PCR) using only a centrifuge and a heater. Additionally, we discovered that short-chain DNA could be efficiently purified by incorporating a concentration step using cationic particles. Quantitative PCR (qPCR) analysis of the purified DNA demonstrated that use of the developed method effectively decreased the DNA detection limit. Overall, this method enables the rapid and inexpensive purification of DNA, and it is suitable for combination with recent advanced DNA analysis technologies such as qPCR, next-generation sequencing, and mass spectrometry. It is therefore expected to contribute to the early detection of cancer and have a major impact on the medical field.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microwave-derivatized enhanced-spectrofluorophotometric characterization of nateglinide-loaded solid dispersion adsorbate and greenness profile assessment of method.","authors":"Pintu Prajapati, Anzarul Haque, Mohd Abul Kalam, Shailesh Shah","doi":"10.1007/s44211-024-00694-5","DOIUrl":"https://doi.org/10.1007/s44211-024-00694-5","url":null,"abstract":"<p><p>Nateglinide is an oral meglitinide that treats diabetes. Nateglinide was determined in several commercial formulations using chromatographic and spectrophotometric methods in published research. Quality-by-design approach was applied to develop the in-house nateglinide-loaded Solid Dispersion Adsorbate (SDA) for improving bioavailability and solubility. To determine nanogram-level nateglinide concentration for the in-house developed nateglinide-SDA pharmacokinetics required time-consuming and expensive hyphenated analytical techniques such as LC-MS or UPLC-MS. Hence, a sensitive spectrofluorophotometric method was developed to estimate nateglinide using NBD-Cl (7-chloro-4-nitrobenzoxadiazole) as the fluorophore. Microwave-aided chemical derivatization of nateglinide with NBD-Cl sped up sample analysis. Chemical reaction solvents were eco-friendly to safeguard aquatic life and environment. The chemical process was optimized by quality by design approach using screening design and response surface modelling. The fluorescence intensity of nateglinide was found to be linear over the concentration range of 20-250 ng/mL with a correlation coefficient of 0.9978. The limits of detection and quantification were found to be 10 and 20 ng/mL respectively. Precision and robustness investigations of the spectrofluorimetric method showed less than 2% relative standard deviations. Nateglinide-SDA drug-release study and pharmacokinetics were performed using the sensitive spectrofluorophotometric method. The in-house developed nateglinide-SDA released 95% of the drug in 30 min time duration. In-house-developed nateglinide-SDA has 1.5 times the oral bioavailability of commercial formulations due to in-vivo absorption. The published analytical methods were compared to the enhanced-spectrofluorophotometric method for user-friendliness, efficiency, cost-of-analysis, and environmental friendliness. The proposed sensitive-spectrofluorophotometric method was found to be robust, rapid, eco-friendly, cost-effective, and simple for the estimation of nateglinide.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Line-dropped gelatin multi-element calibration standards in LA-ICP-MS: a statistically verifying comparison with cryosectioned homogenized lung and liver as matrix-matched calibration standards and as corresponding reference materials.","authors":"Sven Thoröe-Boveleth, Ruth Becker, Jens Bertram, Thomas Schettgen, Manfred Möller, Danny Jonigk, Thomas Kraus, Ralf Weiskirchen","doi":"10.1007/s44211-024-00691-8","DOIUrl":"https://doi.org/10.1007/s44211-024-00691-8","url":null,"abstract":"<p><p>Calibrations in LA-ICP-MS are typically very time-consuming and complex, as they need to be matched to the samples being measured and sectioned on a microtome. Alternatively, gelatin can be in droplet form or as a section, which is a more recent development. In this study, we report on investigations where hot multi-element gelatin solutions are placed in a linear fashion on microscopic slides to conduct comparative statistical observations between doped tissue homogenates from the liver and lung. The tissue homogenates served as both samples (complete ablation) and calibration standards (partial ablation) for verification purposes. We explored the effects of different laser fluences (0.50-1.50 J/cm²), gelatin contents (0.3-20.0%) and section thicknesses (10-30 µm). To do this, we evaluated the samples by calculating median and mean values over the entire section with and without removal of elementary spikes (de-spiking). A reduction in distribution was achieved with averaging. The data was normalized using ¹³C as an internal standard. In these experiments and under these measurement conditions, it was observed that the selected laser fluences, gelatin contents, and section thicknesses did not visibly affect the results, making them comparable. Each sample could be assessed with each gelatin calibration, allowing for determination of expected reference values. Despite interruptions in the measurement operation, due to the high number of measurements, where samples and calibrations could not be analyzed in one measurement run, no negative effects of stopping and starting the LA-ICP-MS were observed.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time-domain nuclear magnetic resonance for serum analysis","authors":"Tatsuya Umecky","doi":"10.1007/s44211-024-00679-4","DOIUrl":"10.1007/s44211-024-00679-4","url":null,"abstract":"","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"40 12","pages":"2099 - 2100"},"PeriodicalIF":1.8,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Femtosecond Raman-induced Kerr effect spectroscopic study of the intermolecular dynamics in aqueous solutions of imidazolium hydrochloride, imidazole, sodium triazolide, and triazole: concentration dependence.","authors":"Masako Shimizu, Hideaki Shirota","doi":"10.1007/s44211-024-00692-7","DOIUrl":"https://doi.org/10.1007/s44211-024-00692-7","url":null,"abstract":"<p><p>In this study, we employed femtosecond Raman-induced Kerr effect spectroscopy to analyze the concentration-dependent intermolecular dynamics in positively or negatively charged aromatics and their neutral analogous aromatics (imidazolium hydrochloride (ImHCl), imidazole (Im), sodium triazolide (NaTr), and triazole (Tr)) in aqueous solutions at 293 K. We also measured their liquid properties, such as density, viscosity, and surface tension, at 293 K, and compared them with their dynamic properties. Furthermore, we performed the quantum chemistry calculations of the target aromatics and some clusters to elucidate their optimized structures, interaction energies, charge populations, and Raman-active normal modes. We characterized the Kerr transients over 2 ps using a triexponential function. The results revealed that the aqueous solutions' intermediate and slow relaxation time constants were linearly proportional to the viscosities. The slopes of the time constants to the viscosity of the aqueous ImHCl solutions were steeper than those of the aqueous Im solutions, whereas the slopes of the aqueous NaTr solutions were milder than those of the aqueous Tr solutions. These findings indicated that the charge of the aromatics in the aqueous solutions affected the coupling parameter between the solute and solvent in the orientational dynamics with different ways. The first moment (M₁) of the low-frequency band (< 200 cm^-1), coming from the intermolecular vibrations, in the difference spectra between the aqueous aromatic solutions and neat water shifted to the high-frequency region as the concentration increased. The M₁ slope to the concentration for the aqueous ImHCl solutions was steeper than that for the aqueous Im solutions. Conversely, the concentration dependence of M₁ for the aqueous NaTr solutions was similar to that for the aqueous Tr solutions. We used the local structures of the target aromatics based on the quantum chemistry calculations to rationally clarify their concentration-dependent intermolecular dynamics in the aqueous solutions.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric quantification of vancomycin by highly active nitroxyl radical compounds.","authors":"Kyoko Sugiyama, Fumiya Sato, Kentaro Yoshida, Sachiko Komatsu, Tetsuya Ono, Yusuke Sasano, Yoshiharu Iwabuchi, Tsutomu Fujimura, Yoshitomo Kashiwagi, Katsuhiko Sato","doi":"10.1007/s44211-024-00686-5","DOIUrl":"10.1007/s44211-024-00686-5","url":null,"abstract":"<p><p>Nitroxyl radicals, represented by 2,2,6,6-tetramethylpiperidine N-oxyl (TEMPO), are highly stable organic free radicals with unique properties and are used as functional molecules in various fields. However, TEMPO had low reactivity and sometimes did not provide enough response. Therefore, highly active nitroxyl radical compounds have been developed in which bicyclo and tricyclo structures stabilize the radicals. In this study, we found that nortropine N-oxyl (NNO), a type of highly active nitroxyl radical, can oxidize the 2,2'-dihydroxybiphenyl structure under physiological conditions, and succeeded in the colorimetric quantification of vancomycin containing 2,2'-dihydroxybiphenyl moieties in the molecular structure. The reaction took only a few minutes to complete and could be confirmed with the naked eye, with a quantitative range of 10-100 μM. High-performance analytical probes are expected to be developed that use highly active nitroxyl radical derivatives to replace TEMPO derivatives.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Room temperature quantitative liquid concentration device and application to interleukins analysis in a B-cell culture medium.","authors":"Ruriko Kawanabe, Hidekatsu Tazawa, Kazuma Mawatari, Ayumi Yoshizaki","doi":"10.1007/s44211-024-00688-3","DOIUrl":"https://doi.org/10.1007/s44211-024-00688-3","url":null,"abstract":"<p><p>In biological analysis and medical diagnosis, there is an increasing demand for improving the lower detection limit without deteriorating the quantitativity; however, it is usually challenging. In this study, we utilized a cyclone flow device and established a liquid concentration method. An air cyclone flow induced a liquid cyclone flow in the concentration devices and enhanced the air/liquid interface area, which allowed an effective concentration of liquid from mL to mL at room temperature. The heating temperature and actual temperature inside the liquid were investigated to know the cooling effect of evaporation. The collection efficiency of larger than 98% was confirmed with a standard solution. Finally, the analytical procedure to realize a quantitative concentration was established, and the concentration and quantification of interleukins (IL-8, IL-17, and IL-23) from the supernatant of the B-cell culture medium was demonstrated. The B-cell was stimulated with CD40L, and the supernatant was concentrated 27 times at maximum.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triple-template surface imprinted magnetic polymers for wide-coverage extraction of steroid hormones from human serum.","authors":"Li Wang, Hongyu Wu, Caihong Wang, Yunfei Ma, Zheng Xiang","doi":"10.1007/s44211-024-00690-9","DOIUrl":"https://doi.org/10.1007/s44211-024-00690-9","url":null,"abstract":"<p><p>In this study, novel triple-template magnetic nanospheres by surface imprinting, called triple-template magnetic molecularly imprinted polymers (tri-MMIPs), were prepared for the detection of steroid hormones in human serum. The polymers were constructed by Fe₃O₄ as support, estrone (E1), progesterone (PROG), and estradiol (E2) as triple templates, acrylic acid (AA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The resulting tri-MMIPs were further characterized and applied as sorbents in human serum pretreatment. Coupled with ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry (UHPLC-MS/MS) profiling after derivatization, 15 steroids (E1, 6-ketoE1, 4-OHE1, 16α-OHE1, DHEA, 4-MeOE1, PROG, PREG, 17-OHPROG, 7-DHE2, E2, 2-OHE2, 16-epiE3, E3, 2-MeOE2) were successfully quantified. The developed method exhibited a good recovery (78.9-114%) and low limit of quantitation (LLOQ, 5-10 pg/mL), which indicates a great potential for application in the analysis of multiple steroid hormones in complex biological samples.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}