Analytical Sciences最新文献_第2页

Advances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus. 金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌快速检测方法研究进展。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-09-06 DOI: 10.1007/s44211-025-00843-4

Linlin Zhuang, Mengling Zhu, Xinya Chen, Xiaoming Liu, Tianyu Yuan, Chuang Zhou, Xue Lian, Yunming Xu, Yu Zhang, Qiuping Shen

{"title":"Advances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus.","authors":"Linlin Zhuang, Mengling Zhu, Xinya Chen, Xiaoming Liu, Tianyu Yuan, Chuang Zhou, Xue Lian, Yunming Xu, Yu Zhang, Qiuping Shen","doi":"10.1007/s44211-025-00843-4","DOIUrl":"https://doi.org/10.1007/s44211-025-00843-4","url":null,"abstract":"<p><p>Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) are important pathogens that are closely associated with hospital-acquired and community-acquired infections. Rapid and accurate detection methods are important for early diagnosis and treatment of diseases. This paper reviews the latest research progress in the field of rapid detection of S. aureus and MRSA, including chromogenic media, immunological detection methods (e.g., enzyme-linked immunosorbent assays, lateral flow assays, colorimetric/fluorescent immunoassays, etc.), nucleic acid tests (including polymerase chain reaction-based methods, isothermal amplification techniques), biosensors, and novel emerging methods, etc. The advantages and disadvantages of these methods are systematically analyzed and discussed. Future research should focus on improving analytical sensitivity and specificity, shortening detection time, reducing detection cost, and promoting the integration of multiple platforms and intelligent analysis, especially for the development of technologies for point-of-care testing. These efforts are crucial to addressing the pressing need for rapid clinical diagnosis.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In ovo sexing of chicken embryos using spectroscopic analysis 用光谱分析测定鸡胚胎的卵性别

IF 2 4区化学

Analytical Sciences Pub Date : 2025-09-03 DOI: 10.1007/s44211-025-00824-7

Mika Ishigaki

引用次数: 0

Investigation of experimental factors in in-capillary continuous enzyme assay to obtain plateau response by electrophoretically mediated microanalysis. 毛细管内连续酶分析中电泳介导微量分析获得平台响应的实验因素研究。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-08-28 DOI: 10.1007/s44211-025-00844-3

Toshio Takayanagi, Risa Okazaki, Karen Gotoda, Masanori Mine, Hitoshi Mizuguchi

{"title":"Investigation of experimental factors in in-capillary continuous enzyme assay to obtain plateau response by electrophoretically mediated microanalysis.","authors":"Toshio Takayanagi, Risa Okazaki, Karen Gotoda, Masanori Mine, Hitoshi Mizuguchi","doi":"10.1007/s44211-025-00844-3","DOIUrl":"https://doi.org/10.1007/s44211-025-00844-3","url":null,"abstract":"<p><p>In-capillary enzymatic reaction was investigated in electrophoretically mediated microanalysis to achieve plateau response of the reaction product. Hydrolysis of 4-nitrophenyl phosphate (NPP) with alkaline phosphatase (ALP) was used as a model enzymatic reaction. The reaction product of 4-nitrophenol (NP) was photometrically detected as a plateau response based on the continuous enzymatic reaction in the separation capillary and the ongoing electrophoretic resolution of the product from the reaction zone. Two types of injection were compared between an enzyme injection and a substrate injection, whereas the counterpart reagent was contained in the separation buffer. In both approaches, the plateau response of NP developed linearly with increasing sample injection period, as well as the enzyme concentration. At low separation voltages, NP accumulated at the reaction zone, and the plateau height was inversely proportional to the applied voltage. Comparing enzyme and substrate injection methods, a stable plateau response was more easily achieved with the enzyme injection. It is because the concentration of the substrate in the injected plug decreases during the electrophoresis, leading to a reduced reaction rate. In contract, enzyme injection maintains consistent reaction conditions. The Michaelis-Menten constant was determined with the plateau response by both injection modes, and the values were comparable with each other.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review of methods for the determination of cellular cholesterol content: principles, advantages, limitations, applications, and perspectives. 细胞胆固醇含量测定方法综述：原理、优点、局限性、应用和展望。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-08-27 DOI: 10.1007/s44211-025-00842-5

Zihang Zhou, Jiangyu Zong, Ning Xu

{"title":"Review of methods for the determination of cellular cholesterol content: principles, advantages, limitations, applications, and perspectives.","authors":"Zihang Zhou, Jiangyu Zong, Ning Xu","doi":"10.1007/s44211-025-00842-5","DOIUrl":"https://doi.org/10.1007/s44211-025-00842-5","url":null,"abstract":"<p><p>Imbalances in cellular cholesterol homeostasis are associated with various diseases, and accurate determination of cholesterol levels and distribution is essential for a thorough understanding of cellular physiopathology. In this review, we comprehensively analyzed various techniques for cellular cholesterol determination. They include indirect methods based on SREBP2 activity monitoring, gas chromatography-liquid chromatography coupling, enzyme analysis, improved Abell-Kendall method, cholesterol-specific probes such as filipin III and cholesterol-dependent cytolysin, and cholesterol analogs such as dehydroergosterol, BODIPY-cholesterol, label-free as well as labeling Raman assays and mass spectrometry imaging techniques such as matrix-assisted laser desorption/ionization mass spectrometry imaging, desorption electrospray ionization mass spectrometry imaging, and nanoscale secondary ion mass spectrometry technology. Principles, advantages, and limitations of each technique are discussed in detail, their characteristics in terms of sensitivity, spatial resolution, and temporal resolution are compared in detail. Finally, suggestions for selecting the technique for different experimental objectives are also given. These findings will help researchers choose the most suitable method according to their own needs, provide strong support for cellular cholesterol research, and promote the development of related fields to better elucidate the significance of cellular cholesterol in normal biologic activities and also its intrinsic relationship with diseases.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laser-induced acoustic desorption coupled with dielectric barrier discharge ionization mass spectrometry for analysis of thermally labile compounds 激光诱导声解吸耦合介质阻挡放电电离质谱分析热不稳定化合物。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-08-22 DOI: 10.1007/s44211-025-00827-4

Xue Xiao, Dan Li, Xiandong Li, Pinchun Kang, Qiao Lu

{"title":"Laser-induced acoustic desorption coupled with dielectric barrier discharge ionization mass spectrometry for analysis of thermally labile compounds","authors":"Xue Xiao, Dan Li, Xiandong Li, Pinchun Kang, Qiao Lu","doi":"10.1007/s44211-025-00827-4","DOIUrl":"10.1007/s44211-025-00827-4","url":null,"abstract":"<div><p>Laser-induced acoustic desorption (LIAD) coupled with dielectric barrier discharge ionization mass spectrometry (DBDI-MS) has been developed as a novel technique for the direct detection of solid samples. LIAD is a “soft” desorption method that enables the volatilization of thermally labile and involatile compounds without significant degradation, while DBDI provides a gentle ionization process. Compared with laser desorption DBDI-MS (LD-DBDI-MS) or laser desorption ionization-MS (LDI-MS), this combination allows for the analysis of fragile molecules with minimal fragmentation. In this study, we optimized the LIAD-DBDI-MS system by adjusting key parameters such as the distance between the titanium foil and the ion source inlet, and the laser power density. The system was successfully employed for the analysis of melamine, copper phthalocyanine, and folic acid, demonstrating its capability to detect molecular species primarily as intact [M + H]⁺ or [M]⁻ ions with minimal or no fragmentation. The results highlight the potential of LIAD-DBDI-MS as a powerful tool for the analysis of complexes and fragile compounds in various fields, including pharmaceuticals and materials science.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 10","pages":"1637 - 1645"},"PeriodicalIF":2.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent progress in functional materials for electrochemical detection of pesticides: modification strategies, performance, and future challenges 农药电化学检测功能材料的最新进展：改性策略、性能和未来挑战。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-08-22 DOI: 10.1007/s44211-025-00838-1

Alvin Lim Teik Zheng, Ellie Yi Lih Teo, Yiu Pang Hung, Juniza Md Saad, Nor Hisam Zamakshshari, Eric Lim Teik Chung, Mohammed Abdillah Ahmad Farid, Yoshito Andou

{"title":"Recent progress in functional materials for electrochemical detection of pesticides: modification strategies, performance, and future challenges","authors":"Alvin Lim Teik Zheng, Ellie Yi Lih Teo, Yiu Pang Hung, Juniza Md Saad, Nor Hisam Zamakshshari, Eric Lim Teik Chung, Mohammed Abdillah Ahmad Farid, Yoshito Andou","doi":"10.1007/s44211-025-00838-1","DOIUrl":"10.1007/s44211-025-00838-1","url":null,"abstract":"<div><p>Widespread pesticides use in agriculture has led to significant groundwater and food contamination, posing severe risks to both human health and the environment. Real-time detection of these emerging contaminants remains a formidable challenge. Electrochemical sensors and biosensors have remained a viable technique for overcoming the limits ascribed to their ultra-sensitivity and rapid detection times as well as excellent sensitivity, reliability, and selectivity. This comprehensive review will encompass the most recent advancements in the utilization of functional materials such as metal–organic framework, MXene, carbon organic framework, carbon nanotubes, and graphene modifiers for the detection of a myriad of pesticides via electrochemical detection techniques, electrode materials, electrolyte solutions, and sample matrices. This review compiles performance data, including detection limits and linear ranges, for various sensing platforms. Finally, the write-up will discuss advanced sensor technology and address the improvements made on electrochemical sensors and biosensors for pesticide detection, which will be important in future studies on pesticide determination.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 10","pages":"1585 - 1606"},"PeriodicalIF":2.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current status of detection strategies for pathogenic Escherichia coli: a brief review and summary 致病性大肠杆菌检测策略的现状：简要回顾与总结。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-08-21 DOI: 10.1007/s44211-025-00836-3

Xudong Xue, Min He, Peng Wang, Yan Zhang

{"title":"Current status of detection strategies for pathogenic Escherichia coli: a brief review and summary","authors":"Xudong Xue, Min He, Peng Wang, Yan Zhang","doi":"10.1007/s44211-025-00836-3","DOIUrl":"10.1007/s44211-025-00836-3","url":null,"abstract":"<div><p>Pathogenic <i>E. coli</i>, a significant concern for researchers and health organizations worldwide, has caused numerous large-scale bacterial infection deaths worldwide. Low-dose infection and high pathogenicity underscore the need for rapid and accurate detection of <i>E. coli</i> to control its infection and protect human health and public health safety. This review outlines the main detection strategies for <i>E. coli</i> in recent years and divides them into six categories: traditional plate colony counting, immunology, optics, molecular biology, electrochemical sensing, and microchip technology. Recent representative articles on <i>E. coli</i> detection are also summarized and analyzed, and their advantages and disadvantages are discussed. Additionally, this review also presents the future direction and prospects of <i>E. coli</i> detection strategies. The main objective of this paper is to provide a comprehensive overview and constructive ideas to develop efficient, advanced, and immediate detection methods for <i>E. coli</i> and pathogenic microorganism detection, emphasizing the need for such strategies.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 10","pages":"1575 - 1584"},"PeriodicalIF":2.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative analysis using LA-ICP-MS and powder samples dispersed in polyvinyl alcohol films. 用LA-ICP-MS和分散在聚乙烯醇薄膜中的粉末样品进行定量分析。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-08-19 DOI: 10.1007/s44211-025-00835-4

Masahiro Kobayashi, Hiromasa Namiki, Hideo Hayashi

{"title":"Quantitative analysis using LA-ICP-MS and powder samples dispersed in polyvinyl alcohol films.","authors":"Masahiro Kobayashi, Hiromasa Namiki, Hideo Hayashi","doi":"10.1007/s44211-025-00835-4","DOIUrl":"10.1007/s44211-025-00835-4","url":null,"abstract":"<p><p>The combination of laser ablation and inductively coupled plasma mass spectrometry (LA-ICP-MS) is a powerful tool for direct analysis of solid samples. However, quantitative analyses by LA-ICP-MS require matrix-matched standard materials, which are not always available. In this study, we have developed a method that enables quantitative analyses of powder samples using LA-ICP-MS. The sample powder is dispersed in a polyvinyl alcohol (PVA) paste, which is a hydrophilic resin, and a film sample is obtained by a screen-printing technique. Analyte concentrations can be determined by a standard-addition method using an analyte-spiked film prepared by mixing standard solutions into the PVA paste. Because of its hydrophilicity, it is possible to increase the ratio of analytes (standard solutions) to the resin. Despite samples being diluted with the resin, this method has enough sensitivity to detect elements at concentrations < 0.1 μg/g. Several types of ceramic powder (SiC, ZrO<sub>2</sub>, and TiO<sub>2</sub>) were analyzed using this method, and all the analytes, the concentrations of which differed by five orders of magnitude (from < 1 μg/g to > 1 wt%), were accurately quantified. This method, in which reliable matrix-matched standard materials are easily tailored for each sample, can be potentially important in quantitative analyses using LA-ICP-MS.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling the combined influence of maturation and organic matter content on terahertz dielectric properties of oil shales. 模拟成熟度和有机质含量对油页岩太赫兹介电性质的综合影响。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-08-19 DOI: 10.1007/s44211-025-00834-5

Federico Sanjuan, Charles Aubourg, Bertrand Fasentieux, Maria Angels Subirana, François Baudin, Maxime Bernier

{"title":"Modeling the combined influence of maturation and organic matter content on terahertz dielectric properties of oil shales.","authors":"Federico Sanjuan, Charles Aubourg, Bertrand Fasentieux, Maria Angels Subirana, François Baudin, Maxime Bernier","doi":"10.1007/s44211-025-00834-5","DOIUrl":"10.1007/s44211-025-00834-5","url":null,"abstract":"<p><p>Maturation and organic matter content are critical factors influencing the hydrocarbon generation potential and economic viability of oil shales. Accurate assessment of these parameters is essential for optimizing extraction techniques and evaluating resource quality. While previous studies have individually linked maturation and organic content to dielectric permittivity in the terahertz (THz) range, their combined influence has not yet been thoroughly investigated. In this study, we explore the interdependence between maturation, organic matter content, and THz dielectric properties. Fifteen oil shale samples from two distinct basins were analyzed using Rock-Eval thermal analysis to quantify organic content, vitrinite reflectance to determine thermal maturity, and THz time-domain spectroscopy to estimate dielectric permittivity. Based on these analyses, we propose an empirical model that links these three parameters through a fitted surface, achieving a 75% accuracy in data representation. These findings provide a more robust framework than previous approaches for characterizing oil shales and enhancing resource evaluation.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple and rapid TLC blotting-based methodology for screening membrane protein-specific lipids 基于薄层色谱印迹的膜蛋白特异性脂质的简单快速筛选方法。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-08-19 DOI: 10.1007/s44211-025-00841-6

Supakorn Wangamnuayporn, Nobuaki Matsumori

{"title":"Simple and rapid TLC blotting-based methodology for screening membrane protein-specific lipids","authors":"Supakorn Wangamnuayporn, Nobuaki Matsumori","doi":"10.1007/s44211-025-00841-6","DOIUrl":"10.1007/s44211-025-00841-6","url":null,"abstract":"<div><p>We present a novel methodology for the rapid and simplified screening of membrane protein (MP)-specific lipids, utilizing thin-layer chromatography (TLC) to separate lipid mixtures, followed by colorimetric detection using our recently developed MP-immobilized gold nanoparticles (AuNPs). After separation, the lipids on the TLC plate were transferred to a polyvinylidene difluoride (PVDF) membrane and visualized colorimetrically with MP-functionalized AuNPs. Lipid species on the TLC or PVDF membrane were further annotated via mass spectrometry. To enhance the visualization of specifically interacting lipids and minimize non-specific binding on the PVDF membrane, several factors, including AuNP size, blocking with bovine serum albumin, and detergent washing, were optimized using bacteriorhodopsin (bR)-immobilized AuNPs. Consequently, S-TGD-1, a known bR-specific lipid, was strongly detected, confirming the effectiveness of this method. We then applied this approach to the potassium channel KcsA and demonstrated that cardiolipin band on the PVDF was most strongly stained by KcsA-immobilized AuNPs. This is consistent with previous reports identifying cardiolipin as a KcsA-specific lipid. To more quantitatively identify MP-specific lipids, we also introduced the Binding Index (BI), which reproduced that S-TGD-1 and cardiolipin are specific to bR and KcsA, respectively. Thus, the utility of this methodology was demonstrated through studies with bR and KcsA. This technique offers a broadly applicable approach for identifying MP-binding lipids without requiring extensive lipid purification or sophisticated instrumentation, providing a versatile tool for studying MP–lipid interactions.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 10","pages":"1669 - 1679"},"PeriodicalIF":2.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0