Analytical Sciences最新文献_第3页

Microfluidic technologies for protein crystallography: advances and applications. 蛋白质晶体学的微流控技术：进展与应用。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-21 DOI: 10.1007/s44211-025-00767-z

Masatoshi Maeki, Akihiko Ishida, Manabu Tokeshi

{"title":"Microfluidic technologies for protein crystallography: advances and applications.","authors":"Masatoshi Maeki, Akihiko Ishida, Manabu Tokeshi","doi":"10.1007/s44211-025-00767-z","DOIUrl":"https://doi.org/10.1007/s44211-025-00767-z","url":null,"abstract":"Three-dimensional protein structure determination by X-ray crystallography is essential for understanding biological function and accelerating drug discovery. However, obtaining high-quality protein crystals remains a significant bottleneck. The conventional crystallization methods are often labor-intensive, require large sample volumes, and offer limited control over the crystallization environment. This review summarizes the application of microfluidic technologies to protein crystallography with a focus on their advantages over the conventional crystallization methods. Microfluidic devices enable nanoliter-scale sample handling, precise control over crystallization conditions, and high-throughput screening, addressing major limitations of the conventional approaches. This review introduces various microfluidic platforms, including droplet-based and microwell-based systems, for protein crystallization, crystal growth control, and on-chip X-ray diffraction analysis. The review also covers the use of microfluidics for creating diffusion-controlled crystal growth environments, real-time crystal growth measurement, on-chip X-ray diffraction measurement, and room-temperature X-ray crystallography with automated data processing.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-sensitive biosensor detection of microRNA based on the CRISPR/Cas12a system and exonuclease-assisted target recycling signal amplification. 基于CRISPR/Cas12a系统和外切酶辅助靶标循环信号放大的microRNA超灵敏生物传感器检测。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-21 DOI: 10.1007/s44211-025-00755-3

Xing Yang, Yu Luo, Chunhua Su, Zhimei Huang, Yafang Tang, Liwen Zhang

{"title":"Ultra-sensitive biosensor detection of microRNA based on the CRISPR/Cas12a system and exonuclease-assisted target recycling signal amplification.","authors":"Xing Yang, Yu Luo, Chunhua Su, Zhimei Huang, Yafang Tang, Liwen Zhang","doi":"10.1007/s44211-025-00755-3","DOIUrl":"https://doi.org/10.1007/s44211-025-00755-3","url":null,"abstract":"MicroRNAs (miRNAs) are essential regulators of gene expression and are significantly involved in both preventing and treating a range of diseases. To that end, we developed an ultra-sensitive detection method for miRNA-141 by integrating exonuclease-assisted target recycling signal amplification with the CRISPR/Cas12a system. This method employs a variable hairpin probe (HP) designed to hybridize with miRNA, which, under the action of exonuclease III (ExoIII), cleaves the hairpin probe and triggers target recycling signal amplification. This results in the formation of output DNAs (ODs) containing multiple repeat sequences. The CRISPR/Cas12a system identifies these repeated sequences in ODs through its crRNA component, which in turn triggers the trans-cleavage function of the Cas12a/crRNA complex. It leads to the cleavage of a fluorescently quenched reporter probe. Consequently, this process restores fluorescence, producing a significantly enhanced fluorescent signal that facilitates the detection of miRNA-141, achieving a detection threshold down to 62 fM. This detection approach can specifically differentiate miRNA-141 from other confounding substances and has effectively identified low concentrations of miRNA-141 in actual sample human serum and diverse cancer cell lysates, showcasing its capability for tracing various nucleic acid biomarkers at minimal levels.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Raman spectroscopy for the quantitative and qualitative analyses of solid drug formulations of salbutamol. 固体剂型沙丁胺醇的拉曼光谱定量和定性分析。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-20 DOI: 10.1007/s44211-025-00760-6

Syeda Takmeel Zahra, Noor Ul Sabah, Muhammad Irfan Majeed, Haq Nawaz, Nosheen Rashid, Abdulrahman Alshammari, Norah A Albekairi, Arslan Ali, Sadia Arshad, Ammara Rehman, Aleena Shahzadi, Sonia Yaseen, Muhammad Kashif, Saira Dastgir, Maria Ghafoor

{"title":"Raman spectroscopy for the quantitative and qualitative analyses of solid drug formulations of salbutamol.","authors":"Syeda Takmeel Zahra, Noor Ul Sabah, Muhammad Irfan Majeed, Haq Nawaz, Nosheen Rashid, Abdulrahman Alshammari, Norah A Albekairi, Arslan Ali, Sadia Arshad, Ammara Rehman, Aleena Shahzadi, Sonia Yaseen, Muhammad Kashif, Saira Dastgir, Maria Ghafoor","doi":"10.1007/s44211-025-00760-6","DOIUrl":"10.1007/s44211-025-00760-6","url":null,"abstract":"Inhalable drugs are commonly used to treat different kinds of respiratory disorders, such as asthma, coughing, wheezing, and breathing difficulties. Salbutamol is an inhalable drug which is used to treat symptoms of acute obstructive lungs disease and asthma. In this research, Raman spectroscopy technique has been utilized to determine the quality and quantity of solid dosage forms of salbutamol drug. Eight formulations of solid drug Salbutamol, combination of excipients and API, were prepared and analyzed. The Raman spectral features showed significant change in their intensities as directly related to variation in the concentration of formulation. Raman spectral results were further assessed by principal component analysis (PCA) and partial least squares regression analysis (PLSR) which are very potent chemometric tools for the quantitative and qualitative analyses of the pharmaceutical drugs. PCA was found to be useful in discriminating the acquired Raman spectra of various salbutamol drug formulations in solid forms of dosage. Furthermore, PLSR model developed for analyzing the quantitative relationship between different formulated solid dosages of salbutamol drug. The prediction of model and the prediction of an unknown drug formulation's concentration is performed by PLSR model. A value of 0.9079 mg was found to be for the root mean square error of cross-validation (RMSECV) and good fit regression R2 was found to be 0.998. Moreover, API concentration (w/w) of unknown formulation of the drug was found to be 8.90 mg (w/w) having actual concentration of 8 mg (w/w).","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic paper-based analytical devices: proven applications and future prospects in therapeutic drug monitoring, homeland security, and chemical education. 微流控纸基分析装置：在治疗药物监测、国土安全和化学教育方面的成熟应用和未来前景。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-19 DOI: 10.1007/s44211-025-00758-0

Manabu Tokeshi

引用次数: 0

Elemental and isotopic analyses of individual nanoparticles using single particle inductively coupled plasma mass spectrometry. 使用单粒子电感耦合等离子体质谱分析单个纳米粒子的元素和同位素。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-19 DOI: 10.1007/s44211-025-00766-0

Masaki Nakazato, Takafumi Hirata

引用次数: 0

Innovations in paper-based analytical devices and portable absorption photometers for onsite analysis. 创新的纸质分析设备和便携式吸收光度计的现场分析。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-18 DOI: 10.1007/s44211-025-00764-2

Sasikarn Seetasang, Mika I Umeda, Jianchao Ren, Takashi Kaneta

引用次数: 0

Breath biomarkers for esophageal cancer: identification, quantification, and diagnostic modeling. 食管癌的呼吸生物标志物：鉴定、量化和诊断模型。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-15 DOI: 10.1007/s44211-025-00769-x

Yuke Ren, Fei Wang, Ziyi Zhu, Raojun Luo, Guojun Lv, Haibin Cui

{"title":"Breath biomarkers for esophageal cancer: identification, quantification, and diagnostic modeling.","authors":"Yuke Ren, Fei Wang, Ziyi Zhu, Raojun Luo, Guojun Lv, Haibin Cui","doi":"10.1007/s44211-025-00769-x","DOIUrl":"https://doi.org/10.1007/s44211-025-00769-x","url":null,"abstract":"Esophageal cancer is a major global health issue with a high mortality rate. Early diagnosis is crucial for improving patient outcomes, but traditional diagnostic methods are often invasive and costly. This study explores the potential of exhaled volatile organic compounds (VOCs) as a non-invasive diagnostic tool for esophageal cancer. Using gas chromatography-mass spectrometry (GC-MS), we analyzed the breath samples of 80 esophageal cancer patients and 60 healthy controls, identifying and quantifying over 100 VOCs. The results revealed significant differences in the concentrations of VOCs such as acetone, ethanol, and isoprene between the two groups. A multi-parameter regression diagnostic model based on a neural network algorithm achieved an accuracy of 90.3% in distinguishing esophageal cancer patients from healthy individuals. Further optimization incorporating physiological factors, including smoking, drinking, and dietary habits, improved the model's accuracy to 92.4%, with a specificity of 93.1%, representing a significant improvement over previous studies. These results suggest that VOCs analysis in exhaled breath holds great promise as a non-invasive, cost-effective, and accurate method for early detection of esophageal cancer.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Confocal micro-X-ray fluorescence and total reflection X-ray fluorescence analyses of Ryugu sample in a laboratory environment. 琉球样品的共聚焦微x射线荧光和全反射x射线荧光分析。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-14 DOI: 10.1007/s44211-025-00753-5

Tsugufumi Matsuyama, Sora Yasuda, Koen Janssens, Lee Wah Lim, Yoshio Takahashi, Kouichi Tsuji

{"title":"Confocal micro-X-ray fluorescence and total reflection X-ray fluorescence analyses of Ryugu sample in a laboratory environment.","authors":"Tsugufumi Matsuyama, Sora Yasuda, Koen Janssens, Lee Wah Lim, Yoshio Takahashi, Kouichi Tsuji","doi":"10.1007/s44211-025-00753-5","DOIUrl":"https://doi.org/10.1007/s44211-025-00753-5","url":null,"abstract":"This study analyzed Ryugu samples utilizing confocal micro-X-ray fluorescence (CM-XRF) and total reflection X-ray fluorescence (TXRF), in which information of elemental mapping as well as the content of trace elements could be obtained separately, and respectively. In CM-XRF, polycapillary lenses are placed in front of the X-ray tube and detector, and elemental information in the overlapping region of both focus points was obtained. Three-dimensional elemental distributions could be obtained by scanning a sample; the Ryugu sample was placed on an Al plate with one center, and the images of Fe, Ni, Cr, and Ca were obtained. The outlines of these elements were almost equivalent; however, as the energy of Ca Kα was low, its intensity in the elemental mapping was weak. Non-destructive TXRF measurements were performed to measure the trace elements in the Ryugu sample. We then performed quantitative analysis and determined the concentrations of trace elements such as Ga, Ge, and Se. The concentrations of these elements are higher than those in the CI chondrites. Therefore, we verified that the 3D elemental distributions and quantitative measurements in the Ryugu sample were performed without destruction.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid determination of total colony counts and prediction of shelf life of dried tofu using LF-NMR. 用LF-NMR快速测定干豆腐菌落总数及预测保质期。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-13 DOI: 10.1007/s44211-025-00759-z

Jian Xiao, Alin Xia, Taidong Hou

{"title":"Rapid determination of total colony counts and prediction of shelf life of dried tofu using LF-NMR.","authors":"Jian Xiao, Alin Xia, Taidong Hou","doi":"10.1007/s44211-025-00759-z","DOIUrl":"https://doi.org/10.1007/s44211-025-00759-z","url":null,"abstract":"Dried tofu is a type of bean food that is popular among Chinese people. However, because dried tofu is usually exposed to air during storage or shelf life, this may lead to a rapid increase in colony counts and spoilage, resulting in shortened shelf life. The traditional method for measuring total colony counts is the plate counting method, which is complex and time consuming. Therefore, it is particularly important to explore a method that can quickly, accurately, and non-destructively determine the total colony counts of dried tofu and predict its shelf life. In this study, the samples were measured by low-field nuclear magnetic resonance (LF-NMR) to obtain transverse relaxation data. Then, the total colony counts in dried tofu was determined by plate counting method, and it was used as a reference value. The backpropagation artificial neural network (BP-ANN) was used to analyze the transverse relaxation data. The results show that the BP-ANN model could quickly and accurately predict total colony counts. In addition, the total colony counts predicted by BP-ANN were used to predict the shelf life. Comparing the predicted shelf life with the actual shelf life of dried tofu. The results show that the relative error between them is less than 10%. Thus, the shelf life model established by the BP-ANN predicted value has a certain reliability. This study provides some references for rapid and nondestructive determination of total colony counts and shelf life prediction of dried tofu.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photothermal radiometric image identification of microplastics through near-infrared excitation. 基于近红外激发的微塑料光热辐射图像识别。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-04-10 DOI: 10.1007/s44211-025-00756-2

Takumi Aramaki, Miki Isoda, Akira Harata

引用次数: 0