Analytical Sciences最新文献_第3页

Plasma separation from whole blood on paper-based fluidic devices. 用纸基流体装置分离全血血浆。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-06-01 DOI: 10.1007/s44211-025-00752-6

Tomoki Yabutani

引用次数: 0

Quantitative analysis of related substances in acetaminophen tablets using pre-column derivatization coupled with HPLC-ICP-MS. 柱前衍生联用HPLC-ICP-MS定量分析对乙酰氨基酚片中有关物质。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-06-01 Epub Date: 2025-03-01 DOI: 10.1007/s44211-025-00739-3

Yalan Liu, Yaru Peng, Qiping Wu, Zeneng Cheng, Feifan Xie, Sanwang Li

{"title":"Quantitative analysis of related substances in acetaminophen tablets using pre-column derivatization coupled with HPLC-ICP-MS.","authors":"Yalan Liu, Yaru Peng, Qiping Wu, Zeneng Cheng, Feifan Xie, Sanwang Li","doi":"10.1007/s44211-025-00739-3","DOIUrl":"10.1007/s44211-025-00739-3","url":null,"abstract":"High-performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) is a promising structure-independent technique for accurate quantification of drug-related substances in pharmaceuticals. In this study, we aimed to develop a sensitive HPLC-ICP-MS method, combined with pre-column derivatization, to quantify related substances in acetaminophen tablets. Through this method, we enhanced the sensitivity and accuracy of detecting substances that do not directly respond to ICP-MS. The key advancements include the systematic optimization of derivatization conditions and the establishment of a calibration curve with correlation coefficients greater than 0.999. The method achieved impressive limits of quantification: 0.089 µM for o-acetaminophenol, 0.097 µM for p-nitrophenol, and 0.161 µM for p-aminophenol. It demonstrated suitable accuracy and precision for the following substances: o-acetaminophenol with accuracy between 95.7-104.4% and precision ≤ 4.9%, p-nitrophenol with accuracy between 97.6-101.8% and precision ≤ 2.7%, and p-aminophenol with accuracy between 95.6-102.5% and precision ≤ 3.2%. Comparative analysis with standard HPLC-UV method revealed less than 20% difference in quantification, underscoring the effectiveness of this combined technique. Our study demonstrated the feasibility of combining chemical derivatization with HPLC-ICP-MS as a robust tool for the accurate quantification of drug-related substances, particularly those lacking elements detectable by ICP-MS alone.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":"813-824"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracting accurate PDF data from in situ environment of materials using X-ray diffractometer. 利用x射线衍射仪从材料的原位环境中提取准确的PDF数据。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-06-01 Epub Date: 2025-02-13 DOI: 10.1007/s44211-025-00728-6

Yuji Shiramata, Takayuki Konya, Kentaro Kobayashi, Yui Ishii, Satoshi Hiroi, Hiroki Yamada, Koji Ohara

{"title":"Extracting accurate PDF data from in situ environment of materials using X-ray diffractometer.","authors":"Yuji Shiramata, Takayuki Konya, Kentaro Kobayashi, Yui Ishii, Satoshi Hiroi, Hiroki Yamada, Koji Ohara","doi":"10.1007/s44211-025-00728-6","DOIUrl":"10.1007/s44211-025-00728-6","url":null,"abstract":"Pair distribution function (PDF) analysis is a technique traditionally used at synchrotron radiation facilities, but it is now possible to obtain adequate measurements using laboratory-based equipment as well. In addition, cryo-furnaces that enable in situ measurements while controlling temperature have been well established for some time. PDF analysis requires data from a wide range of reciprocal space, along with measurements at short wavelengths. However, the development of time-resolved PDF measurements, which can be captured within a short time span, has also made significant progress. In this study, we demonstrate the reliability of PDF measurements using laboratory equipment and perform a local structure analysis of materials exhibiting an interesting structural phase transition in LaCu6-xAgx through temperature-dependent in situ PDF analysis. By comparing these results with those obtained from synchrotron sources, we provide further validation of the laboratory-based measurements. Our results confirm that in situ PDF measurements obtained with laboratory-based equipment are reliable and effective for studying structural disorder in various materials.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":"777-782"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemiluminescence immunoassay using ionic-liquid submicron particles for prostate-specific antigen determination. 离子液体亚微米粒子电化学发光免疫法测定前列腺特异性抗原。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-06-01 Epub Date: 2025-02-21 DOI: 10.1007/s44211-025-00734-8

Aki Tanaka, Yuki Kiguchi, Shigehiko Takegami

{"title":"Electrochemiluminescence immunoassay using ionic-liquid submicron particles for prostate-specific antigen determination.","authors":"Aki Tanaka, Yuki Kiguchi, Shigehiko Takegami","doi":"10.1007/s44211-025-00734-8","DOIUrl":"10.1007/s44211-025-00734-8","url":null,"abstract":"In this study, ionic-liquid submicron particles (ILSPs) encapsulating the luminophore tris(2',2-bipyridyl)ruthenium (II) ([Ru(bpy)3]2+) were developed as a carrier for an electrochemiluminescence immunoassay (ECLIA). The ILSPs were applied to quantitative determination of the model analyte prostate-specific antigen (PSA). The electrochemiluminescence of [Ru(bpy)3]2+ was measured with 2-(dibutylamino)ethanol as a co-reactant in nine ionic liquids (ILs). The electrochemiluminescence intensity was higher in 1-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide ([BMIM][TFSA]) and N-(2-methoxyethyl)-N-methyl pyrrolidinium bis(trifluoromethanesulfonyl)imide ([MEMP][TFSA]) than the other ILs. ILSPs were prepared using polyoxyethylene sorbitan monolaurate and sorbitan monooleate as surfactants and either [BMIM][TFSA] or [MEMP][TFSA]. The [BMIM][TFSA] ILSPs had a mean particle size of 244 nm and zeta potential of - 21.0 mV, and the [MEMP][TFSA] ILSPs had a mean particle size of 293 nm and zeta potential of - 17.9 mV. Microscope images showed that ILSPs were IL-in-water emulsions that completely encapsulated [Ru(bpy)3]2+. The ILSPs with [BMIM][TFSA] were more stable than those with [MEMP][TFSA], and [BMIM][TFSA] ILSPs was selected as a carrier in ECLIA for PSA determination. The calibration curve of PSA for ECLIA using the [BMIM][TFSA] ILSPs showed a good linear relationship (y = 0.29x + 4.02, r = 0.95) for the PSA concentration range of 100 pg/mL-100 μg/mL. The limit of detection and limit of quantification were 544 pg/mL and 35 ng/mL, respectively. Our results demonstrate that ECLIA using ILSPs can be used to easily determine the PSA concentration even with ILSPs in the particle state.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":"793-801"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Raman spectroscopic analysis of carbonates on a CY-type carbonaceous chondrite Yamato 980115: unexpectedly abundant terrestrial weathering products. cy型碳质球粒陨石Yamato 980115碳酸盐的拉曼光谱分析：意外丰富的陆地风化产物。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-06-01 Epub Date: 2025-02-24 DOI: 10.1007/s44211-025-00731-x

Shu-Hei Urashima, Hayato Tsuchiya, Naoya Imae, Akira Yamaguchi, Hiroharu Yui

{"title":"Raman spectroscopic analysis of carbonates on a CY-type carbonaceous chondrite Yamato 980115: unexpectedly abundant terrestrial weathering products.","authors":"Shu-Hei Urashima, Hayato Tsuchiya, Naoya Imae, Akira Yamaguchi, Hiroharu Yui","doi":"10.1007/s44211-025-00731-x","DOIUrl":"10.1007/s44211-025-00731-x","url":null,"abstract":"This study presents Raman spectra of carbonates on a carbonaceous chondrite, Yamato (Y) 980115. Y 980115 is an Antarctic meteorite and is originally classified as CI1-type. Since CI1-type meteorites are expected to record information of aqueous alteration of carbonaceous matters in the primitive solar photosphere or planetesimals, extensive studies have been performed. Among a variety of minerals on meteorites, carbonates are one of environment indicators because its cation composition depends on thermochemical properties of the aqueous solution when they were precipitated. Although the minerals on meteorites are usually identified by elemental composition analysis techniques such as backscattered electron imaging, these techniques have a drawback that they lack information of crystal structures so that it is difficult to distinguish some minerals such as ordered or disordered dolomite (CaMg(CO3)2) and a mixture of calcite (CaCO3) and magnesite (MgCO3). The present study employed Raman spectroscopy as a complementary technique to the elemental composition analysis. As a result, surprisingly, while the Mg-bearing carbonates on Y 980115 were expected mainly to be dolomite, the Raman spectra revealed that they are indeed nesquehonite (Mg(HCO3)(OH)·2H2O), which is a typical terrestrial weathering product for Antarctic meteorites. Among 63 carbonate grains found on Y 980115, 60 and 3 grains were assigned to nesquehonite and calcite, respectively, and no dolomite was found. This result indicates that there are unexpectedly abundant terrestrial weathering products for carbonates found on Y 980115.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":"689-695"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of a large antigen through the masking and exposure of a fragment of split luciferase. 通过掩蔽和暴露一个分裂的荧光素酶片段来检测一个大抗原。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-06-01 Epub Date: 2025-04-08 DOI: 10.1007/s44211-025-00754-4

Cheng Qian, Ayumu Ninomiya, Natsuki Shibukawa, Hiroshi Ueda, Takanobu Yasuda, Bo Zhu, Tetsuya Kitaguchi

{"title":"Detection of a large antigen through the masking and exposure of a fragment of split luciferase.","authors":"Cheng Qian, Ayumu Ninomiya, Natsuki Shibukawa, Hiroshi Ueda, Takanobu Yasuda, Bo Zhu, Tetsuya Kitaguchi","doi":"10.1007/s44211-025-00754-4","DOIUrl":"10.1007/s44211-025-00754-4","url":null,"abstract":"We developed PMBiT, an antibody-binding Protein M (PM)-based bioluminescent probe that detects large antigens via luciferase reconstitution by exposing a luciferase fragment. Detection is achieved by exploiting the principle that the antibody, large antigen, and PM cannot form a complex simultaneously. PMBiT was prepared by conjugating PM with a HiBiT-based peptide from split NanoLuc luciferase through an Azide-DBCO click reaction. It retained its binding activity to the antibody and showed bioluminescence upon reconstitution of the luciferase with LgBiT, the other fragment of the split NanoLuc. Mixing PMBiT with various IgG antibodies resulted in decreased bioluminescence. In contrast, when PMBiT was mixed with IgG bound to its large antigen, such as human C-reactive protein, a dose-dependent increase in bioluminescence was obtained. Molecular dynamics simulations of PM showed that two regions in the C-terminus contribute to steric clashes with antigens owing to their relatively rigid structures. Furthermore, in silico analysis of the structure suggested that the antigen size was the primary factor blocking the binding of PMBiT to IgG for antigen detection. An immunoassay utilizing PMBiT does not require genetic manipulation of antibodies, allowing for seamless and scalable antibody replacement, and will advance the future of on-site detection and rapid diagnostics.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":"857-865"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of the damaging effect of IR radiation on DNA by electrochemical methods and investigation of this effect in the presence of chitosan. 电化学法测定红外辐射对DNA的破坏作用，并在壳聚糖的存在下研究这种影响。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-06-01 Epub Date: 2025-02-17 DOI: 10.1007/s44211-025-00727-7

Pelin Uysal Çalıcıoğlu, Fatma Akpınar, Kübra Gençdağ Şensoy, Şerife Gökçe Çalışkan, Mihrican Muti

{"title":"Determination of the damaging effect of IR radiation on DNA by electrochemical methods and investigation of this effect in the presence of chitosan.","authors":"Pelin Uysal Çalıcıoğlu, Fatma Akpınar, Kübra Gençdağ Şensoy, Şerife Gökçe Çalışkan, Mihrican Muti","doi":"10.1007/s44211-025-00727-7","DOIUrl":"10.1007/s44211-025-00727-7","url":null,"abstract":"Infrared (IR) rays released from natural and artificial sources reach the layers of the skin and cause different effects. The most important effects of IR rays are the deterioration of the structure of proteins in the skin and the inadequacy of the skin's defense system. Early diagnosis of DNA damage caused by IR rays and discovery of products with antioxidant properties that will reduce the effects of damage are important in the prevention and treatment of possible diseases. This study aimed to determine the harmful effects of IR rays on DNA by measuring the guanine oxidation signal. Within the scope of the study, electrodes with DNA immobilized on their surfaces were exposed to IR irradiation and the change in guanine oxidation was detected electrochemically. A 57% decrease in guanine oxidation signal was observed as a result of IR exposure, and this rate decreased in the presence of chitosan. The results obtained show that chitosan is effective in preventing DNA damage caused by IR rays and suggest that chitosan-supported products can be used to protect the skin from the harmful effects of sun rays. Furthermore, molecular docking analyses indicated no interaction between chitosan and collagen protein. This suggests that the addition of chitosan to any cosmetic product applied to the skin is unlikely to induce DNA damage attributable to chitosan.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":"783-792"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The latest developments of near-infrared fluorescent probes from NIR-I to NIR-II for bioimaging. 生物成像用近红外荧光探针从nir - 1到NIR-II的最新进展。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-06-01 Epub Date: 2025-02-28 DOI: 10.1007/s44211-025-00735-7

Yuzuka Kuronuma, Ryuji Watanabe, Yuki Hiruta

引用次数: 0

Ratiometric electrochemical aptasensor based on functionalized graphene nanocomposites for detection of CA19-9. 基于功能化石墨烯纳米复合材料的比例电化学感应传感器检测CA19-9。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-06-01 Epub Date: 2025-03-06 DOI: 10.1007/s44211-025-00743-7

Feifei Lin, Xiaosha Li, Jian Zhang, Hailin Zhang, Zemiao Zhang, Lijie Hou, Bingjun Shen, Lihong Jin

{"title":"Ratiometric electrochemical aptasensor based on functionalized graphene nanocomposites for detection of CA19-9.","authors":"Feifei Lin, Xiaosha Li, Jian Zhang, Hailin Zhang, Zemiao Zhang, Lijie Hou, Bingjun Shen, Lihong Jin","doi":"10.1007/s44211-025-00743-7","DOIUrl":"10.1007/s44211-025-00743-7","url":null,"abstract":"A ratiometric electrochemical aptasensor was constructed based on rGO-cMWCNTs/AuNPs functionalized graphene nanocomposites for the detection of CA19-9 in serum samples. First, rGO-cMWCNTs/AuNPs nanocomposites were modified on the surface of glassy carbon electrodes. Second, the aptamer complementary chain labeled with anthraquinone-2-carboxylic acid (cDNA-AQ) was covalently linked by Au-S. Finally, the hybrid chain was formed with ferrocene (Fc)-labeled Apt-Fc by base pairing, and a sensing interface with specific target recognition and electrochemical signal amplification was constructed. In this study, the ratio of response signal to internal reference signal (IFc/IAQ) was used as the target molecular response signal to achieve the quantitative detection of CA19-9. The ratiometric electrochemical biosensor based on aptamer molecular recognition has good selectivity, reproducibility and stability. Under the optimal reaction conditions, the logarithm of CA19-9 concentration had a good linear relationship with IFc/IAQ, the linear range was 1.0-1.0 × 106 mU/mL, the detection limit was 0.65 mU/mL, and the recovery rate in CA19-9 serum was 94.12-97.83%. The results show that the sensor has potential practical application prospects, and provides a simple and feasible strategy for the rapid and accurate detection of other tumor markers.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":"825-838"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of iodide in concentrated chloride springs by reversed-phase high-performance liquid chromatography with ultraviolet detection. 反相高效液相色谱-紫外检测法测定浓氯化物泉中的碘。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-06-01 Epub Date: 2025-02-23 DOI: 10.1007/s44211-025-00732-w

Tomoko Kemmei, Azusa Yudo, Shuji Kodama, Atsushi Yamamoto, Yoshinori Inoue, Shigehiro Kagaya, Kazuichi Hayakawa

引用次数: 0