Analytical Sciences最新文献_第3页

Chemiluminescence detection of kanamycin by DNA aptamer regulating peroxidase-like activity of Co3O4 nanoparticles. 通过 DNA 配体调节 Co3O4 纳米粒子的过氧化物酶样活性对卡那霉素进行化学发光检测。

IF 1.6 4区化学

Analytical Sciences Pub Date : 2024-09-17 DOI: 10.1007/s44211-024-00672-x

Xuxin Zhang,Yihao Li,Shaojie Xia,Zhenyuan Yang,Baiyun Zhang,Yonghong Wang

{"title":"Chemiluminescence detection of kanamycin by DNA aptamer regulating peroxidase-like activity of Co3O4 nanoparticles.","authors":"Xuxin Zhang,Yihao Li,Shaojie Xia,Zhenyuan Yang,Baiyun Zhang,Yonghong Wang","doi":"10.1007/s44211-024-00672-x","DOIUrl":"https://doi.org/10.1007/s44211-024-00672-x","url":null,"abstract":"Kanamycin (KAN) is widely used as a growth hormone analog and an antibacterial agent. However, abuse of this substance has resulted in the accumulation of excessive residue levels in foods of animal origin, which presents a significant risk to human health. A chemiluminescent aptasensor was constructed for the rapid quantitative detection of KAN by combining the properties of Co3O4 nanoparticles (Co3O4 NPs) nanozyme activity and DNA aptamer with high specificity. The DNA aptamer/Co3O4 NPs nanozyme regulated the chemiluminescence signal by exploiting the chemiluminescent properties of luminol oxidation by H2O2. Specific binding of KAN to the aptamer led to the formation of a steric hindrance block in the solution, which inhibited the activity of nanozyme and reduced signal intensity. The degree of signal reduction is related to the concentration of KAN. Under optimal conditions, there was good linearity between KAN concentration and chemiluminescence signal intensity in the range of 0.5-8.0 μΜ, with a detection limit of 0.26 μΜ. The detection system performed well in the presence of competing antibiotics and was virtually unaffected. The method was also suitable for the detection of KAN in milk samples with sample recoveries of 97.8%-99.1%. The chemiluminescence sensor has the advantages of low cost, specificity, and sensitivity, and does not require an external light source or modification of the nucleic acid aptamer which makes it a promising candidate for applications in the field of food detection.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"53 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142258156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lateral flow immunoassay device to detect staphylococcal enterotoxin B (SEB) in durian candy. 检测榴莲糖中的葡萄球菌肠毒素 B (SEB) 的侧流免疫测定装置。

IF 1.6 4区化学

Analytical Sciences Pub Date : 2024-09-13 DOI: 10.1007/s44211-024-00665-w

Paquito E Relox,Katherine Ann C Israel,Yuki Hiruta,Daniel Citterio

{"title":"Lateral flow immunoassay device to detect staphylococcal enterotoxin B (SEB) in durian candy.","authors":"Paquito E Relox,Katherine Ann C Israel,Yuki Hiruta,Daniel Citterio","doi":"10.1007/s44211-024-00665-w","DOIUrl":"https://doi.org/10.1007/s44211-024-00665-w","url":null,"abstract":"Staphylococcal enterotoxin B (SEB), a potent enterotoxin produced by Staphylococcus aureus, has been implicated in incidences of Staphylococcal food poisoning in the Philippines. The use of lateral flow immunoassay devices to detect this toxin in solid food samples, like durian candy, at the point of sampling is constrained by the requirement for sample purification (e.g. centrifugation). This problem is also true with the other applications of LFIA devices on food samples. To overcome this challenge, a lateral flow immunoassay (LFIA) device capable of detecting SEB in unpurified durian candy sample was developed in this study. A modified LFIA device was assembled with three layers of glass fiber pads functioning as sample pads instead of a conventional cellulose fiber pad. Unlike with the cellulose fiber pad, the glass fiber sample pads acted as filter and allowed the flow of a 1:5 dilution of durian candy. The LFIA device applied to spiked 1:5 diluted durian candy samples achieved a visual limit of detection of 5 ng/mL for SEB, which is twofold lower than reported for previous LFIA devices designed to detect SEB in food samples.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"15 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142258155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-tech innovation: biomimetic solid-contact potentiometric sensor for nanomolar-level atrazine detection. 低技术创新：用于检测纳摩尔级莠去津的仿生物固体接触电位传感器。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-09-12 DOI: 10.1007/s44211-024-00664-x

Anjana Kolarveetil Rajagopalan, Saumya Varghese, Aneesh Padmanabhan, Theertha Thayyullathil

{"title":"Low-tech innovation: biomimetic solid-contact potentiometric sensor for nanomolar-level atrazine detection.","authors":"Anjana Kolarveetil Rajagopalan, Saumya Varghese, Aneesh Padmanabhan, Theertha Thayyullathil","doi":"10.1007/s44211-024-00664-x","DOIUrl":"https://doi.org/10.1007/s44211-024-00664-x","url":null,"abstract":"Despite the fact that there are already a number of solid-contact-based ion-selective electrodes designed for atrazine detection, our ground-breaking contribution lies in introducing the first-ever atrazine potentioselectrode, enabling the ultra-sensitive detection of atrazine at nanomolar levels. Solid-contact ion-selective electrodes can offer advantages, such as improved stability, reproducibility, sensitivity, and selectivity compared to their liquid-contact counterparts. Here, a biomimetic potentiometric sensor for Atrazine was developed using economic, light weight, and flexible carbon cloth as solid-contact material. Our methodology entails the synthesis of a molecularly imprinted polymer (MIP) through straightforward precipitation polymerization, showcasing a streamlined and efficient method for creating highly specific molecular recognition elements. The validation of template removal is confirmed via meticulous analysis employing EDX and FTIR techniques, ensuring the efficacy of our methodology. The resulting sensing membrane are casted by dispersing the MIP in 2-nitrophenyl octyl ether plasticizer and embedding it within a PVC matrix containing sodium tetraphenyl borate as a lipophilic additive. The developed sensor responds to atrazine in the pH range of 2.8-3.3 over a wide concentration range of 1 × 10-8 M to 1 × 10-5 M & 1 × 10-5 M to 1 × 10-1 M with respective slopes of 29.2 mv & 58.7 mV and a limit of detection of 1 × 10-9 M. An impressive feature of this sensor lies in its swift response time, registering a rapid reaction within a mere 10 s. Emphasize the sensor's commendable attributes of reproducibility, selectivity, and sensitivity underscoring its successful application in field monitoring.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A QuEChERS method based on octadecyl-bonded hectorite for the determination of ten mycotoxins in yak ghee. 基于十八烷基键合蛭石的 QuEChERS 方法测定牦牛酥油中的十种霉菌毒素。

IF 1.6 4区化学

Analytical Sciences Pub Date : 2024-09-11 DOI: 10.1007/s44211-024-00667-8

Jun Wang,Hai-Yan Yang,Jia-Jia Fan,Zi-Han Xu,Zai-Hui Pang,Yuan Feng,Na Wei

{"title":"A QuEChERS method based on octadecyl-bonded hectorite for the determination of ten mycotoxins in yak ghee.","authors":"Jun Wang,Hai-Yan Yang,Jia-Jia Fan,Zi-Han Xu,Zai-Hui Pang,Yuan Feng,Na Wei","doi":"10.1007/s44211-024-00667-8","DOIUrl":"https://doi.org/10.1007/s44211-024-00667-8","url":null,"abstract":"To develop a clean-up material suitable for high-fat food matrices for detecting mycotoxins in yak ghee, an octadecyl-bonded hectorite (Hectorite@NHCO(CH2)17CH3) was synthesized through multi-step chemical reactions. A modified QuEChERS-HPLC-MS/MS method for detecting ten mycotoxins in sesame oil in yak ghee was established using Hectorite@NHCO(CH2)17CH3 as clean-up agent. It involved extracting mycotoxin contaminants using acidified acetonitrile and employing the Hectorite@NHCO(CH2)17CH3 to remove interfering substances from the extract. The purified samples were then analyzed using HPLC-MS/MS. Within a linear range of 1.0-500 μg/kg, there was a good linear relationship between the quantification ion peak area of the target analytes and the corresponding concentrations (R2 ≥ 0.9991). The limit of detection (LOD) ranged from 0.10 μg/kg to 18.62 μg/kg and the limit of quantitation (LOQ) ranged 0.32-62.07 μg/kg. The recoveries at low, medium and high concentrations (25, 100 and 500 μg/kg) ranged from 72.2% to 113.9%, with relative standard deviations (RSD) between 3.2% and 17.5%. The intra-day and inter-day precision met experimental requirements. The proposed method was characterized by a high accuracy and precision, and it could cater to the current demand for detecting ten mycotoxins in yak ghee.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"72 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142185250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potentiometry in determining the antioxidant capacity of compounds of different hydrophilicity in the joint presence using mixed solvents and surfactants. 使用混合溶剂和表面活性剂测定不同亲水性化合物抗氧化能力的电位计。

IF 1.6 4区化学

Analytical Sciences Pub Date : 2024-09-10 DOI: 10.1007/s44211-024-00662-z

Elena Salimgareeva,Violetta Starcheusova,Elena Gerasimova,Daria Gordeeva,Alla Ivanova

{"title":"Potentiometry in determining the antioxidant capacity of compounds of different hydrophilicity in the joint presence using mixed solvents and surfactants.","authors":"Elena Salimgareeva,Violetta Starcheusova,Elena Gerasimova,Daria Gordeeva,Alla Ivanova","doi":"10.1007/s44211-024-00662-z","DOIUrl":"https://doi.org/10.1007/s44211-024-00662-z","url":null,"abstract":"In this work, for the first time, the potentiometric method was used to determine the antioxidant capacity (AOC) of compounds of different hydrophilicity in the joint presence using mixed solvents and surfactants. The AOC of model solutions of antioxidants of different hydrophilicity was determined separately and in the joint presence in the media of phosphate buffer-surfactant and mixed solvents-surfactant, using as an example the ascorbic acid and the α-tocopherol. It was shown that the surfactant Triton X-100 is able to solubilize α-tocopherol under the selected conditions, allows to obtain reproducible and accurate results, and has less effect on the equilibrium rate of the K3[Fe(CN)6]/K4[Fe(CN)6] system. Phosphate buffer-ethanol and phosphate buffer-acetonitrile media in the 3:2 ratio with Triton X-100 (5 mmol/dm3) were chosen as the analysis conditions (RSD ≤ 6%). The range of determined concentrations was (0.006-0.5) m mmol/dm3 in phosphate buffer-ethanol and (0.006-0.3) m mmol/dm3 in phosphate buffer-acetonitrile. AOC of raw materials infusions was determined in selected media. The positive correlation with the well-known Folin-Ciocalteu assay was obtained.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"310 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142224260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of stopped-flow hyper-CEST NMR method on recirculating hyperpolarization system as applied to void space analysis in polymers. 开发应用于聚合物空隙分析的循环超极化系统停流超 CEST NMR 方法。

IF 1.6 4区化学

Analytical Sciences Pub Date : 2024-09-10 DOI: 10.1007/s44211-024-00661-0

Hideaki Fujiwara,Hirohiko Imai,Atsuomi Kimura

{"title":"Development of stopped-flow hyper-CEST NMR method on recirculating hyperpolarization system as applied to void space analysis in polymers.","authors":"Hideaki Fujiwara,Hirohiko Imai,Atsuomi Kimura","doi":"10.1007/s44211-024-00661-0","DOIUrl":"https://doi.org/10.1007/s44211-024-00661-0","url":null,"abstract":"129Xe NMR spectroscopy of polymers can provide important information on void spaces, sometimes called free volume, in polymers. Unfortunately, the spectroscopy's low sensitivity has limited its widespread use in both academic and industrial research. In order to overcome such a difficult situation, hyper-CEST method which employs hyperpolarization and CEST techniques, is examined after the introduction of recirculation and subtraction modes. Alongside the incorporated stopped-flow technique, these modes were very efficient in detecting very weak hidden signals from cellulose nanofiber (CNF) and silk fibroin (SF) films and in discussing the void space in these polymers. From the analysis of detailed saturation frequency dependence in the increment of 100 Hz, the chemical shifts of hidden peaks were successfully determined to give reasonable values for the size of void space in CNF and SF. Application on thermoplastic polyurethane film also supported our method of analysis. The subtraction mode was very efficient in judging the presence or absence of any peak at a fixed saturation frequency. These facts support that the mode will surely be useful in the future exploratory study of very weak hidden signals.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"36 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142185251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of cell signaling profiles induced by DNA aptamer-based FGFR1 agonist. 基于 DNA aptamer 的 FGFR1 激动剂诱导的细胞信号谱分析。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-09-09 DOI: 10.1007/s44211-024-00660-1

Junya Hoshiyama, Yuri Hayata, Akihiro Eguchi, Jumpei Morimoto, Ryosuke Ueki, Shinsuke Sando

{"title":"Analysis of cell signaling profiles induced by DNA aptamer-based FGFR1 agonist.","authors":"Junya Hoshiyama, Yuri Hayata, Akihiro Eguchi, Jumpei Morimoto, Ryosuke Ueki, Shinsuke Sando","doi":"10.1007/s44211-024-00660-1","DOIUrl":"https://doi.org/10.1007/s44211-024-00660-1","url":null,"abstract":"DNA aptamers have attracted attention as an alternative modality for biomolecules due to their excellent target binding specificity and thermal stability, and they are also expected to be applied as artificial agonists for receptor proteins. DNA aptamer agonist TD0 targeting the receptor of fibroblast growth factor (FGFR), which plays an important role in the fields of wound healing and regenerative medicine, has been reported to induce cellular responses as well as its native ligands. However, it was also noted that there were some different responses upon long-term stimulation, suggesting that the intracellular signals induced by DNA aptamer agonist TD0 are different from those of natural ligands. In this paper, we comprehensively analyzed the intracellular signals induced by DNA aptamer agonist TD0 targeting FGFR1, and compared them with those by natural protein ligand FGF2. It was found that the intracellular signals were highly similar for short-term stimulation. On the other hand, the receptor and the downstream cellular signals showed different activation behaviors for long-time stimulation. Evaluating the stability and sustained activity of DNA aptamer agonist TD0 and FGF2 in the medium suggested that ligand stability may be important in properly regulating cellular responses.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Size-dependent As(V) adsorption of reduced graphene oxide/magnetite nanocomposites. 还原氧化石墨烯/磁铁矿纳米复合材料对 As(V) 的吸附与尺寸有关。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-09-06 DOI: 10.1007/s44211-024-00657-w

Duc Dung Mai, Thanh Loan To, Thi Hang Bui, Thi Kim Lien Nguyen, Thi Kim Phuong Luong, Thi Lan Nguyen

引用次数: 0

Fabrication of sulphur-doped graphitic carbon nitride anchored Ag@AgCl electrocatalyst for the sensing of chloramphenicol. 用于氯霉素传感的掺硫氮化石墨碳锚定 Ag@AgCl 电催化剂的制备。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-09-06 DOI: 10.1007/s44211-024-00658-9

Suparna Kallakkattil, Yarradoddappa Venkataramanappa

{"title":"Fabrication of sulphur-doped graphitic carbon nitride anchored Ag@AgCl electrocatalyst for the sensing of chloramphenicol.","authors":"Suparna Kallakkattil, Yarradoddappa Venkataramanappa","doi":"10.1007/s44211-024-00658-9","DOIUrl":"https://doi.org/10.1007/s44211-024-00658-9","url":null,"abstract":"We have developed sulphur-doped graphitic carbon nitride (S-GCN) anchored Ag@AgCl electrocatalyst through a green technique for the first time for the electrochemical sensing of chloramphenicol. The Ag@AgCl nanoparticles were synthesized using Rhoeo discolor (Tradescantia spathacea) plant extract without the use of any external halide source. As per our knowledge, this is the first time Rhoeo discolor (Tradescantia spathacea) plant extract was used for the synthesis of Ag@AgCl nanoparticles without the use of any external halide source. Using sonochemical technique, the green synthesized nanoparticle was combined with S-GCN to form Ag@AgCl/S-GCN electrocatalyst. The synthesized materials were characterized by suitable techniques such as UV-visible spectroscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and elemental analysis. The electrocatalytic reduction mechanism of chloramphenicol was studied with the help of electrochemical impedance spectroscopy, cyclic voltammetry, and linear sweep voltammetry. The Ag@AgCl/S-GCN modified electrode has shown a linear response in the range of 1 to 650 μM, with a LOD of 420 <math><mtext>nM</mtext></math> . Further, the practical application of the developed sensor was analyzed using real samples such as milk and honey and satisfactory recovery rates were observed.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142144979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fluorescent probe for monitoring carboxylesterases in pulmonary cells under permissive hypercapnia condition. 在允许性高碳酸血症条件下监测肺细胞中羧酸酯酶的荧光探针。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-09-06 DOI: 10.1007/s44211-024-00659-8

Xiao-Ping Wu, Na Yang, Qing-Qing Liu, Zhong-Quan Zhu

引用次数: 0