Analytical Sciences最新文献_第3页

The usage of color coordinates preserved in microscopic image for elucidation on chemical behavior in confined micro-/nano-spaces.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-02-22 DOI: 10.1007/s44211-025-00733-9

Arinori Inagawa

{"title":"The usage of color coordinates preserved in microscopic image for elucidation on chemical behavior in confined micro-/nano-spaces.","authors":"Arinori Inagawa","doi":"10.1007/s44211-025-00733-9","DOIUrl":"https://doi.org/10.1007/s44211-025-00733-9","url":null,"abstract":"<p><p>Characterizing the properties of micro-/nanomaterials and micro-/nanospaces is crucial due to their widespread use in diverse applications, including analytical methods. While microspectroscopy offers a powerful characterization technique, limitations in acquisition speed and instrument accessibility persist. This study comprehensively explores the use of color coordinates to elucidate the chemical phenomena within confined micro-/nanospaces. Encoding spectral information directly as color coordinates within digital images enables rapid data acquisition. In addition, simple instrumentation that requires only a camera as a detector expands the versatility of solving tasks that occur in microspectroscopy. This work details the fundamental principles of color coordinates and presents illustrative research examples demonstrating their utility in characterizing chemical reactions and physicochemical properties within confined spaces. Herein, the evaluation of the chemical reactions occurring in the confined microspace is explained, including the steel corrosion occurring in the freeze concentration of the salt solutions (FCS) formed in the frozen salt solutions, proton-involved reactions in the FCS, liquid/liquid and solid/gel interface. This work aims to promote the adoption of image processing for colorimetric analysis as a complementary or alternative approach to conventional microspectroscopy.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemiluminescence immunoassay using ionic-liquid submicron particles for prostate-specific antigen determination.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-02-21 DOI: 10.1007/s44211-025-00734-8

Aki Tanaka, Yuki Kiguchi, Shigehiko Takegami

{"title":"Electrochemiluminescence immunoassay using ionic-liquid submicron particles for prostate-specific antigen determination.","authors":"Aki Tanaka, Yuki Kiguchi, Shigehiko Takegami","doi":"10.1007/s44211-025-00734-8","DOIUrl":"https://doi.org/10.1007/s44211-025-00734-8","url":null,"abstract":"<p><p>In this study, ionic-liquid submicron particles (ILSPs) encapsulating the luminophore tris(2',2-bipyridyl)ruthenium (II) ([Ru(bpy)<sub>3</sub>]<sup>2+</sup>) were developed as a carrier for an electrochemiluminescence immunoassay (ECLIA). The ILSPs were applied to quantitative determination of the model analyte prostate-specific antigen (PSA). The electrochemiluminescence of [Ru(bpy)<sub>3</sub>]<sup>2+</sup> was measured with 2-(dibutylamino)ethanol as a co-reactant in nine ionic liquids (ILs). The electrochemiluminescence intensity was higher in 1-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide ([BMIM][TFSA]) and N-(2-methoxyethyl)-N-methyl pyrrolidinium bis(trifluoromethanesulfonyl)imide ([MEMP][TFSA]) than the other ILs. ILSPs were prepared using polyoxyethylene sorbitan monolaurate and sorbitan monooleate as surfactants and either [BMIM][TFSA] or [MEMP][TFSA]. The [BMIM][TFSA] ILSPs had a mean particle size of 244 nm and zeta potential of - 21.0 mV, and the [MEMP][TFSA] ILSPs had a mean particle size of 293 nm and zeta potential of - 17.9 mV. Microscope images showed that ILSPs were IL-in-water emulsions that completely encapsulated [Ru(bpy)<sub>3</sub>]<sup>2+</sup>. The ILSPs with [BMIM][TFSA] were more stable than those with [MEMP][TFSA], and [BMIM][TFSA] ILSPs was selected as a carrier in ECLIA for PSA determination. The calibration curve of PSA for ECLIA using the [BMIM][TFSA] ILSPs showed a good linear relationship (y = 0.29x + 4.02, r = 0.95) for the PSA concentration range of 100 pg/mL-100 μg/mL. The limit of detection and limit of quantification were 544 pg/mL and 35 ng/mL, respectively. Our results demonstrate that ECLIA using ILSPs can be used to easily determine the PSA concentration even with ILSPs in the particle state.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon black imprinted o-diaminobenzene sensing platform for sensitive electrochemical detection of interleukin 6

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-02-21 DOI: 10.1007/s44211-024-00709-1

Xu Cui, Zhiqiang Chen, Longyao Xu, Changwei Wu, Bin Qiu, Chaoming Zhou

{"title":"Carbon black imprinted o-diaminobenzene sensing platform for sensitive electrochemical detection of interleukin 6","authors":"Xu Cui, Zhiqiang Chen, Longyao Xu, Changwei Wu, Bin Qiu, Chaoming Zhou","doi":"10.1007/s44211-024-00709-1","DOIUrl":"10.1007/s44211-024-00709-1","url":null,"abstract":"<div><p>As a typical proinflammatory cytokine, interleukin 6 (IL-6) exhibits great influence on treating diseases (e.g., urocystitis) and adjusting immune. Normally, the IL-6 degree keeps a low content (< 40.0 pg mL<sup>−1</sup>) in bodies, but when its degree becomes higher, several serious conditions (e.g., urocystitis and infections) may occur. Hence, it is very essential to design a highly effective technology for monitoring IL-6 as a biomarker. In the present work, through adopting carbon black (CB) as substrate substance, o-diaminobenzene as electropolymerized monomer and [Fe(CN)<sub>6</sub>]<sup>4−/3−</sup> as the signal probe, a low-cost, sensitive and simple molecularly imprinted polymer (MIP) electrochemical sensing platform was designed to detect the IL-6 degree. The experiments revealed that the current of [Fe(CN)<sub>6</sub>]<sup>4−/3−</sup> probe at the MIP sensor would decrease along with the increase of IL-6 degree. Owing to the prominent properties of CB (e.g., high conductivity and surface area as well as stability) and high target-recognition capability of MIP, the as-proposed MIP sensor offers wide linearity (0.1–800 pg mL<sup>−1</sup>) and low analytical limit (0.03 pg mL<sup>−1</sup>); meanwhile, it possesses good repeatability, stability, and practical applications. It is, thus, expected this simple MIP sensor has some application value for IL-6 detection.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 4","pages":"487 - 494"},"PeriodicalIF":1.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochar-based electrochemical sensors: a tailored approach to environmental monitoring.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-02-18 DOI: 10.1007/s44211-025-00726-8

Alvin Lim Teik Zheng, Ellie Teo Yi Lih, Yiu Pang Hung, Supakorn Boonyuen, Syeed SaifulAzry Osman Al Edrus, Eric Lim Teik Chung, Yoshito Andou

{"title":"Biochar-based electrochemical sensors: a tailored approach to environmental monitoring.","authors":"Alvin Lim Teik Zheng, Ellie Teo Yi Lih, Yiu Pang Hung, Supakorn Boonyuen, Syeed SaifulAzry Osman Al Edrus, Eric Lim Teik Chung, Yoshito Andou","doi":"10.1007/s44211-025-00726-8","DOIUrl":"https://doi.org/10.1007/s44211-025-00726-8","url":null,"abstract":"<p><p>Biochar (BC), often obtained via thermochemical conversion methods of biomass, has emerged as a versatile material with significant potential in electrochemical sensing applications. This review critically examines the recent advancements in the development of BC-based sensors for the electrochemical determination of pharmaceuticals, pesticides, heavy metals, phenolic compounds, and microplastics. BC-based electrochemical sensors have emerged as a promising alternative due to their sustainability, cost-effectiveness, and excellent electrochemical properties. The unique physicochemical properties of BC, including its high surface area, porosity, and functional groups, contribute to its effectiveness as a sensor material. The review begins with an overview of the synthesis methods for BC, highlighting the activation strategies on its structural and electrochemical properties. Next, the functionalization of BC and its integration into electrochemical sensor platforms are explored. The performance of BC-based sensors is evaluated using electrochemical focusing on their sensitivity, selectivity, detection limits, and stability. Future directions for research are proposed, emphasizing the need for further optimization, miniaturization, and integration of BC-based sensors into portable and on-site analytical devices.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Luciferase complementation for cellular assays beyond protein-protein interactions.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-02-18 DOI: 10.1007/s44211-025-00730-y

Genki Kawamura, Takeaki Ozawa

引用次数: 0

Mechanical phenotype of cancer cells and its measurement methodology

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-02-17 DOI: 10.1007/s44211-025-00714-y

Noritada Kaji

引用次数: 0

Determination of the damaging effect of IR radiation on DNA by electrochemical methods and investigation of this effect in the presence of chitosan.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-02-17 DOI: 10.1007/s44211-025-00727-7

Pelin Uysal Çalıcıoğlu, Fatma Akpınar, Kübra Gençdağ Şensoy, Şerife Gökçe Çalışkan, Mihrican Muti

{"title":"Determination of the damaging effect of IR radiation on DNA by electrochemical methods and investigation of this effect in the presence of chitosan.","authors":"Pelin Uysal Çalıcıoğlu, Fatma Akpınar, Kübra Gençdağ Şensoy, Şerife Gökçe Çalışkan, Mihrican Muti","doi":"10.1007/s44211-025-00727-7","DOIUrl":"https://doi.org/10.1007/s44211-025-00727-7","url":null,"abstract":"<p><p>Infrared (IR) rays released from natural and artificial sources reach the layers of the skin and cause different effects. The most important effects of IR rays are the deterioration of the structure of proteins in the skin and the inadequacy of the skin's defense system. Early diagnosis of DNA damage caused by IR rays and discovery of products with antioxidant properties that will reduce the effects of damage are important in the prevention and treatment of possible diseases. This study aimed to determine the harmful effects of IR rays on DNA by measuring the guanine oxidation signal. Within the scope of the study, electrodes with DNA immobilized on their surfaces were exposed to IR irradiation and the change in guanine oxidation was detected electrochemically. A 57% decrease in guanine oxidation signal was observed as a result of IR exposure, and this rate decreased in the presence of chitosan. The results obtained show that chitosan is effective in preventing DNA damage caused by IR rays and suggest that chitosan-supported products can be used to protect the skin from the harmful effects of sun rays. Furthermore, molecular docking analyses indicated no interaction between chitosan and collagen protein. This suggests that the addition of chitosan to any cosmetic product applied to the skin is unlikely to induce DNA damage attributable to chitosan.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracting accurate PDF data from in situ environment of materials using X-ray diffractometer.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-02-13 DOI: 10.1007/s44211-025-00728-6

Yuji Shiramata, Takayuki Konya, Kentaro Kobayashi, Yui Ishii, Satoshi Hiroi, Hiroki Yamada, Koji Ohara

{"title":"Extracting accurate PDF data from in situ environment of materials using X-ray diffractometer.","authors":"Yuji Shiramata, Takayuki Konya, Kentaro Kobayashi, Yui Ishii, Satoshi Hiroi, Hiroki Yamada, Koji Ohara","doi":"10.1007/s44211-025-00728-6","DOIUrl":"https://doi.org/10.1007/s44211-025-00728-6","url":null,"abstract":"<p><p>Pair distribution function (PDF) analysis is a technique traditionally used at synchrotron radiation facilities, but it is now possible to obtain adequate measurements using laboratory-based equipment as well. In addition, cryo-furnaces that enable in situ measurements while controlling temperature have been well established for some time. PDF analysis requires data from a wide range of reciprocal space, along with measurements at short wavelengths. However, the development of time-resolved PDF measurements, which can be captured within a short time span, has also made significant progress. In this study, we demonstrate the reliability of PDF measurements using laboratory equipment and perform a local structure analysis of materials exhibiting an interesting structural phase transition in LaCu<sub>6-x</sub>Ag<sub>x</sub> through temperature-dependent in situ PDF analysis. By comparing these results with those obtained from synchrotron sources, we provide further validation of the laboratory-based measurements. Our results confirm that in situ PDF measurements obtained with laboratory-based equipment are reliable and effective for studying structural disorder in various materials.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in electrochemiluminescence sensing for in vitro cell analysis: a review.

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-02-07 DOI: 10.1007/s44211-025-00723-x

Kosuke Ino, Tomas Mockaitis, Ryota Shikuwa, Kimiharu Oba, Kaoru Hiramoto, Inga Morkvenaite-Vilkonciene, Hiroya Abe, Hitoshi Shiku

{"title":"Recent advances in electrochemiluminescence sensing for in vitro cell analysis: a review.","authors":"Kosuke Ino, Tomas Mockaitis, Ryota Shikuwa, Kimiharu Oba, Kaoru Hiramoto, Inga Morkvenaite-Vilkonciene, Hiroya Abe, Hitoshi Shiku","doi":"10.1007/s44211-025-00723-x","DOIUrl":"https://doi.org/10.1007/s44211-025-00723-x","url":null,"abstract":"<p><p>Electrochemiluminescence (ECL) is a chemiluminescence phenomenon triggered by electrochemical reactions and is widely used for (bio)chemical analyses and electrochemical bioimaging. Compared to fluorescence sensing, ECL sensing reduces background noise by eliminating autofluorescence associated with excitation light. In addition, compared with conventional electrochemical imaging with scanning electrochemical microscopes, ECL imaging is faster as it requires no scanning. Furthermore, unlike electrode arrays, ECL devices can function without complex wiring, simplifying their construction. These characteristics render ECL sensing a useful analytical tool. Recently, ECL sensing has been widely used for in vitro cell analysis due to high demand for biochips in regenerative medicine, drug screening, and microphysiological systems. This review focuses on recent advancements in ECL-based cell analysis with applications for the detection of H<sub>2</sub>O<sub>2</sub>, respiration activity, cell adhesion, lipid membranes, and bipolar electrochemistry-based devices.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accurate evaluation of diffusion coefficient for electroactive analytes in human serum samples using nitrogen-terminated sputtered carbon film electrode

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-02-07 DOI: 10.1007/s44211-025-00718-8

Saki Ohta, Tomonori Ozawa, Shunsuke Shiba, Tatsuhiko Yajima, Tomoyuki Kamata, Dai Kato, Osamu Niwa

{"title":"Accurate evaluation of diffusion coefficient for electroactive analytes in human serum samples using nitrogen-terminated sputtered carbon film electrode","authors":"Saki Ohta, Tomonori Ozawa, Shunsuke Shiba, Tatsuhiko Yajima, Tomoyuki Kamata, Dai Kato, Osamu Niwa","doi":"10.1007/s44211-025-00718-8","DOIUrl":"10.1007/s44211-025-00718-8","url":null,"abstract":"<div><p>We have developed an N-terminated carbon film electrode that allows accurate determination of the diffusion coefficient of electroactive molecules dissolved in a highly concentrated serum protein solution. The carbon film electrode was formed by the unbalanced magnetron sputtering (UBM) method. Then, nitrogen functional groups were introduced by employing NH<sub>3</sub> or H<sub>2</sub>O plasma treatment. Cyclic voltammetry measurements with ferricyanide ion ([Fe(CN)<sub>6</sub>]<sup>3−</sup>) showed that the N-terminated carbon film electrode exhibited great anti-fouling property against simulated serum proteins (50 mg/mL human serum albumin and 15 mg/mL γ-globulin dissolved in 1 M KCl solution). In contrast, glassy carbon, H<sub>2</sub>O plasma-treated, and especially untreated carbon film electrodes were subject to severe electrode fouling, making it difficult to electrochemically determine the diffusion coefficient of the [Fe(CN)<sub>6</sub>]<sup>3−</sup> ion. The control experiment using less adsorptive ethylene glycol as a viscosity modifier showed that the increase in viscosity is a main factor of the decrease in diffusion coefficient for nitrogen plasma treated electrode, which is not significantly affected by the possible interaction between [Fe(CN)<sub>6</sub>]<sup>3−</sup> ions and serum proteins. Finally, we applied the electrode for the electrochemical analysis of acetaminophen dissolved in phosphate buffer (0.1 M, pH = 7.0), which suggests that NH<sub>3</sub> plasma-treated carbon film exhibits the lowest Δ<i>E</i> increase when we compare Δ<i>E</i> with and without proteins and also a more stable peak current in continuous voltametric measurements compared with other carbon electrodes.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 4","pages":"439 - 446"},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0