Analytical Sciences最新文献

{"title":"Nitrogen-doped carbon nanodots as a biocompatible FRET donor for caspase-3 detection and chemotherapy response evaluation in osteosarcoma cell lysates","authors":"Qingwei Wang,&nbsp;Zheng Xu,&nbsp;Deluo Wu,&nbsp;Yao Zhang,&nbsp;Jian’an Li,&nbsp;Fuyuan Ma","doi":"10.1007/s44211-026-00956-4","DOIUrl":"10.1007/s44211-026-00956-4","url":null,"abstract":"<div><p>Osteosarcoma is a highly aggressive bone malignancy requiring precise tools for early diagnosis and effective evaluation of chemotherapy efficacy. Here, we report an “off–on” fluorescent nanosensor based on nitrogen-doped carbon nanodots (N-CNDs) for the sensitive and selective detection of caspase-3 activity, a key apoptosis executor. The sensor is constructed by conjugating carboxyl-functionalized N-CNDs with a BHQ1-labeled peptide substrate containing the caspase-3-specific DEVD cleavage motif. In the absence of caspase-3, fluorescence is quenched via FRET. Upon caspase-3 activation, the peptide is cleaved, releasing BHQ1 and restoring fluorescence. The nanosensor exhibits high sensitivity (detection limit of 0.0106 U/mL), excellent selectivity, and robust performance in complex biological media. As a proof-of-concept application, it detects elevated caspase-3 activity in osteosarcoma cell lysates (MG-63) compared to normal osteoblast lysates (hFOB1.19) and enables quantitative, dose-dependent assessment of apoptosis induced by cisplatin and doxorubicin directly in cancer cell lysates. With its current format, this cell-lysate-based platform provides a practical and sensitive tool for preclinical drug screening and rapid comparison of chemotherapeutic potency, while laying a foundation for future adaptation toward live-cell and in vivo imaging applications.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 10","pages":"993 - 1008"},"PeriodicalIF":2.0,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148710988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applicability of HILIC-SPE pretreatment for the quantitation of N2-Ethyl-2’-deoxyguanosine in LC-ESI/MS/MS","authors":"Hiroya Murakami,&nbsp;Ibuki Sakai,&nbsp;Soo Takasu,&nbsp;Yukihiro Esaka,&nbsp;Yoshinori Inoue,&nbsp;Norio Teshima","doi":"10.1007/s44211-026-00957-3","DOIUrl":"10.1007/s44211-026-00957-3","url":null,"abstract":"<div><p>DNA adducts analysis is crucial for elucidating the mechanism of mutagenesis and carcinogenesis induced by various chemical compounds. Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC–MS/MS) is commonly employed to determine DNA adduct. Pretreatment is essential in DNA adduct analysis due to a significant concentration disparity between normal 2’-deoxynucleosides (dNs) and DNA adducts. The level of DNA adducts is typically assessed by the ratio of DNA adducts to normal 2’-deoxyguanosine (dG) when the DNA adducts originate from dG. Consequently, the determination of dG and DNA adducts is necessary for accurate evaluation. Reversed-phase (RP)-solid-phase extraction (SPE) is the first choice for pretreatment of DNA adducts analysis. This pretreatment using RP-SPE effectively enriches DNA adducts while removing a substantial amount of dNs, particularly thymidine (T) and 2’-deoxyadenosine (dA), which may suppress the ionization of DNA adducts in the electrospray ionization (ESI) process. However, two separate measurements are required to determine the concentrations of dG and DNA adducts, as dG is removed during the SPE process. Therefore, an SPE method that simultaneously extracts dG and DNA adducts is desirable. In this study, we investigated the applicability of hydrophilic interaction chromatography (HILIC)-type SPE sorbents developed by our team, for the pretreatment of DNA adduct analysis. <i>N</i>²-ethyl-2’-deoxyguanosine (EtdG), an acetaldehyde-derived DNA adduct, was selected as a model DNA adduct. By optimizing the washing and eluting solutions, dG and EtdG were simultaneously extracted from the sample solution while eliminating T and dA. This pretreatment method enables the simultaneous measurements of dG and DNA adducts concentration, thereby enhancing the accuracy and rapidity of the DNA adducts analysis.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 10","pages":"1009 - 1016"},"PeriodicalIF":2.0,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148719986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activated carbon–coated particles as adsorbent for packed columns in gas chromatography","authors":"Ikuo Ueta,&nbsp;Takuma Okamura,&nbsp;Masanori Nagao,&nbsp;Naoya Miyajima,&nbsp;Yoshihiro Saito","doi":"10.1007/s44211-026-00952-8","DOIUrl":"10.1007/s44211-026-00952-8","url":null,"abstract":"<div><p>This study reports the first development of gas chromatographic columns packed with activated carbon (AC)-coated adsorbent particles. The AC-coated adsorbent particles were prepared by coating an AC layer on solid support particles using a vacuum carbon coater. Glass beads, diatomite, a porous polymer, and activated alumina particles were investigated as the support materials. The AC layer was uniformly coated on the surface of each support material, achieving a thickness of approximately 300–400 nm after 500 coating cycles. The AC-coated particles were packed in a stainless steel tube with an inner diameter of 1.0 mm and a length of 1.0 m to prepare a packed capillary column. The retention performance of the AC-coated particle-packed columns was evaluated using a capillary gas chromatograph coupled with a flame ionization detector. Strong retention for typical low-molecular-weight organic compounds, including methane, ethane, and propane, was observed. The retention power increased with increasing number of AC-coating cycles and support surface area. The theoretical plate numbers were lower for adsorbents coated on nonporous and low-porous supports, and higher for supports with a large specific surface area. The results demonstrated that the present AC-coating method readily affords AC adsorbents with high retention for typical volatile compounds.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><img></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 10","pages":"1051 - 1056"},"PeriodicalIF":2.0,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148683372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Raman scattering spectroscopic analysis on the structural changes in the micelles of a CTAB-NapTS system","authors":"Ishige Yuki,&nbsp;Kenichi Oguchi,&nbsp;Hiroharu Yui","doi":"10.1007/s44211-026-00950-w","DOIUrl":"10.1007/s44211-026-00950-w","url":null,"abstract":"<div><p>Condensed micelle solutions are ubiquitously seen and utilized in foods, medicine, oil recovery, and so on. It is well-known that micelles formed from ionic surfactants drastically change their aggregate morphologies from spherical to short, rigid rod-like, and occasionally to long, winding worm-like structures as the concentration of the co-existing salts is varied. In general, these large-scale morphological changes induced by additive salt have been directly assessed by X-ray or neutron scattering spectroscopies. However, industrial processes are often carried out under open-system and atmospheric conditions, thus they show transient and dynamic changes of their structures and resultant viscoelastic properties according to the environmental changes. Here we examined whether Raman scattering spectroscopy is applicable for detecting such large-scale morphological changes in micelles by monitoring the corresponding changes occurring in the local packing environment of neighboring surfactant alkyl chains. As a representative micelle-salt system, cetyltrimethylammonium bromide (CTAB) and sodium <i>p</i>-toluene sulfonate (Na<i>p</i>TS) were studied by varying their concentration ratio. As a result, it was proposed that the CH₂ twisting and scissoring modes were useful indices to discriminate the morphological changes in micelles. These results provide a useful index for monitoring the transient structural changes of micelle solutions and elucidating the mechanism of the resultant changes in viscoelastic properties in situ and on-site monitoring.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 10","pages":"953 - 963"},"PeriodicalIF":2.0,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-026-00950-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148676863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solvent-based post-processing to fill layer gaps in FFF-printed polypropylene fluidic chips and their application to flow injection analysis","authors":"Tomohisa Yamashita","doi":"10.1007/s44211-026-00954-6","DOIUrl":"10.1007/s44211-026-00954-6","url":null,"abstract":"<div><p>The development of new analytical tools remains a powerful approach in analytical chemistry, and three-dimensional (3D) printing has gained increasing popularity as a manufacturing technique in recent years. The fabrication of experimental tools using 3D printers has attracted attention in many fields. However, objects fabricated by fused filament fabrication (FFF) may contain gaps between printed paths, and which can cause leakage when liquid is introduced. In this study, solvent-based post-processing was performed in order to fill layer gaps in FFF-printed polypropylene fluidic chips. As methods, a compression process and solvent-based post-processing were applied. In the compression process, a polypropylene fluidic chip was compressed using a compression tool while being heated. After this compression process, solvent-based post-processing was conducted. The post-processed chip was used to measure peak profile during flow-injection measurement. As a result, peak profiles were obtained, and no leakage from the chip was observed during the measurements.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 10","pages":"965 - 974"},"PeriodicalIF":2.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-026-00954-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A bromocresol green electropolymerized film-modified pencil graphite electrode for sensitive differential pulse voltammetric detection of tinidazole","authors":"Mehmet Güneş,&nbsp;Serkan Karakaya,&nbsp;Yusuf Dilgin","doi":"10.1007/s44211-026-00953-7","DOIUrl":"10.1007/s44211-026-00953-7","url":null,"abstract":"<div><p>A novel and highly sensitive electrochemical sensor based on an electropolymerized bromocresol green (BCG) film-modified pencil graphite electrode (poly(BCG)@PGE) was developed for the determination of tinidazole (TNDZ). The electropolymerization of BCG on the electrode surface significantly enhanced the electrochemical response toward TNDZ by improving electron-transfer kinetics and increasing the effective surface area. The fabrication and surface characteristics of the modified electrode were systematically investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and Fourier-transform infrared spectroscopy (FT-IR). The electrochemical behavior of TNDZ was studied using CV and differential pulse voltammetry (DPV). Key experimental parameters affecting sensor performance, including the pH of the electrolyte solution, monomer concentration, and number of electropolymerization cycles, were optimized. Under the optimized conditions, the proposed sensor exhibited a wide linear dynamic range (LDR) from 0.4 to 500 µM for TNDZ determination, with a high sensitivity of 1402 µA·mM⁻¹·cm⁻² and a low limit of detection (LOD) of 0.11 µM. The practical applicability of the developed voltammetric sensor was successfully demonstrated by the determination of TNDZ in artificial blood, pharmaceutical formulation, and spring water samples. The obtained results showed satisfactory recoveries with high precision and accuracy, confirming the reliability and potential applicability of the poly(BCG)@PGE sensor for sensitive electrochemical monitoring of TNDZ in complex matrices.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 10","pages":"975 - 992"},"PeriodicalIF":2.0,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148629221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of trace gold in sub-microliter samples by surface-enhanced laser-induced breakdown spectroscopy using porous silicon with a single laser shot","authors":"Yui Sugita,&nbsp;Ayumu Matsumoto,&nbsp;Hiroto Torigoe,&nbsp;Kiichi Kuroda,&nbsp;Shinji Yae","doi":"10.1007/s44211-026-00951-9","DOIUrl":"10.1007/s44211-026-00951-9","url":null,"abstract":"<div><p>Surface-enhanced laser-induced breakdown spectroscopy (SELIBS) using porous silicon (Si) is an attractive technique for liquid analysis in terms of requiring only microvolume samples, simple pretreatment, and a low plasma generation threshold. These features make SELIBS particularly suitable for industrial process monitoring, such as wet metal recovery processes. In our previous studies, we have analyzed sample solutions with volumes of approximately 10 µL by accumulating multiple spectra measured over the spread area of the sample. Reducing the volume of sample solutions and enabling rapid analysis remain important challenges for practical applications. In this study, we demonstrate single-shot analysis of trace gold (Au) in sub-microliter solution samples by SELIBS using porous Si, representing a step toward practical use. The emission line of Au was successfully observed using only 0.5 µL of sample and a single laser shot with an energy of 2.0 mJ. A calibration curve for Au was built using the Au intensity normalized by the Si intensity in the Au concentration range of 0.5–5.0 ppm (mg/L). From this calibration curve, a coefficient of determination of 0.997 was obtained. The limit of detection was estimated to be 0.48 ppm based on the Au intensity. The relative errors of predicted Au concentrations in additionally analyzed samples using the Au/Si calibration curve were approximately 10%. The proposed method has a potential for in-situ monitoring of wet metal recovery processes.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 10","pages":"941 - 951"},"PeriodicalIF":2.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the bulk: engineering analytical functions with nanofluidics and nanostructures","authors":"Yan Xu","doi":"10.1007/s44211-026-00934-w","DOIUrl":"10.1007/s44211-026-00934-w","url":null,"abstract":"<div><div><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 8","pages":"653 - 655"},"PeriodicalIF":2.0,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148552908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive electrochemical sensing of imidacloprid based on graphdiyne/carbon nanotubes nanohybrids","authors":"Yong Zhao,&nbsp;Jianshan Bai,&nbsp;Youzhi Su,&nbsp;Wenchuan Weng,&nbsp;Zhenghua Xu,&nbsp;Qing Liu","doi":"10.1007/s44211-026-00949-3","DOIUrl":"10.1007/s44211-026-00949-3","url":null,"abstract":"<div><p>As a representative member of neonicotinoid insecticides, imidacloprid (IMI) has seen growing application in agricultural seed coating treatments, raising widespread concerns over its associated environmental accumulation and food chain contamination. Graphdiyne (GDY), an emerging star carbon nanomaterial following graphene, has demonstrated exceptional versatility in analytical sensing; however, its application for electrochemical detection of IMI remains unexplored. Herein, we reported the fabrication and systematic characterization of a novel electrochemical sensing platform based on graphdiyne-coated carbon nanotube (GDY/CNTs) nanohybrids, synthesized via a facile in-situ growth strategy, for sensitive IMI detection. Key operational parameters including PBS buffer pH and GDY/CNTs modification loading were systematically optimized to enhance sensing performance. Under optimal conditions, the GDY/CNTs sensor exhibited a wide linearity of 0.01–10 µM, with a low limit of detection of 3.2 nM and a high sensitivity of 1.4656 µA·µM⁻¹. Importantly, the developed sensor achieved satisfactory recovery rates (≥ 95%) in real sample analysis, revealing its practical applicability. This work established a reliable GDY-based electrochemical approach for rapid, sensitive, and accurate IMI detection in complex matrices, expanding the analytical applications of GDY-based materials.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 10","pages":"1033 - 1039"},"PeriodicalIF":2.0,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148547699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method for correcting the temperature dependence of field-type glass electrode pH sensors","authors":"Kei Okamura,&nbsp;Takuroh Noguchi,&nbsp;Mayumi Hatta","doi":"10.1007/s44211-026-00948-4","DOIUrl":"10.1007/s44211-026-00948-4","url":null,"abstract":"<div><p>Seawater pH measurement is essential for monitoring ocean acidification and its biogeochemical impacts. Glass electrode pH sensors are widely used for in situ applications due to their ease of operation, low power consumption, and no reagent requirements. However, their accuracy can be affected by temperature-dependent effects, particularly those associated with the internal reference solution. Conventional calibration methods based on the Nernst response do not explicitly account for temperature-induced variations in the internal solution, which may lead to systematic bias under conditions with large temperature gradients. In this study, a correction method is proposed to address temperature-dependent errors by modeling the internal pH (pH_in). The model incorporates two primary temperature-dependent contributions: the intrinsic temperature dependence of the phosphate buffer and potential shifts caused by activity changes in saturated KCl. These effects are combined through the electrode response factor (<i>f</i>_R), defined as the ratio of the observed electrode slope to the theoretical Nernst slope, to estimate pH_in as a function of temperature and correct measured pH values to a reference temperature of 25 °C. Application of the method to vertical seawater profiles demonstrated a reduction in deviation from colorimetric reference measurements. The corrected results showed reduced temperature-dependent bias across depth. This approach provides a practical framework for improving the reliability of in situ glass electrode pH measurements under varying thermal conditions. The proposed method addresses equilibrium temperature-dependent effects of the internal solution but does not explicitly account for transient hysteresis, pressure effects, or long-term sensor drift.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 10","pages":"1027 - 1032"},"PeriodicalIF":2.0,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-026-00948-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148534925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}