Analytical Sciences最新文献_第4页

Exploring liquid-liquid phase separation in vitro and in vivo using multimodal nonlinear optical imaging. 利用多模态非线性光学成像技术探索体外和体内液-液相分离。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-06-01 Epub Date: 2025-03-20 DOI: 10.1007/s44211-025-00747-3

Yusuke Murakami, Mia Obuchi, Hiroshi Kamizawa, Shinichi Miyazaki, Akihiro Kishimura, Ryosuke Oketani, Kotaro Hiramatsu, Philippe Leproux, Yu Hayashi, Kentaro Shiraki, Hideaki Kano

引用次数: 0

Ultra-sensitive biosensor detection of microRNA based on the CRISPR/Cas12a system and exonuclease-assisted target recycling signal amplification. 基于CRISPR/Cas12a系统和外切酶辅助靶标循环信号放大的microRNA超灵敏生物传感器检测。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-06-01 Epub Date: 2025-04-21 DOI: 10.1007/s44211-025-00755-3

Xing Yang, Yu Luo, Chunhua Su, Zhimei Huang, Yafang Tang, Liwen Zhang

{"title":"Ultra-sensitive biosensor detection of microRNA based on the CRISPR/Cas12a system and exonuclease-assisted target recycling signal amplification.","authors":"Xing Yang, Yu Luo, Chunhua Su, Zhimei Huang, Yafang Tang, Liwen Zhang","doi":"10.1007/s44211-025-00755-3","DOIUrl":"10.1007/s44211-025-00755-3","url":null,"abstract":"MicroRNAs (miRNAs) are essential regulators of gene expression and are significantly involved in both preventing and treating a range of diseases. To that end, we developed an ultra-sensitive detection method for miRNA-141 by integrating exonuclease-assisted target recycling signal amplification with the CRISPR/Cas12a system. This method employs a variable hairpin probe (HP) designed to hybridize with miRNA, which, under the action of exonuclease III (ExoIII), cleaves the hairpin probe and triggers target recycling signal amplification. This results in the formation of output DNAs (ODs) containing multiple repeat sequences. The CRISPR/Cas12a system identifies these repeated sequences in ODs through its crRNA component, which in turn triggers the trans-cleavage function of the Cas12a/crRNA complex. It leads to the cleavage of a fluorescently quenched reporter probe. Consequently, this process restores fluorescence, producing a significantly enhanced fluorescent signal that facilitates the detection of miRNA-141, achieving a detection threshold down to 62 fM. This detection approach can specifically differentiate miRNA-141 from other confounding substances and has effectively identified low concentrations of miRNA-141 in actual sample human serum and diverse cancer cell lysates, showcasing its capability for tracing various nucleic acid biomarkers at minimal levels.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":"867-876"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent applications of near infrared to pharmaceutical process monitoring and quality control. 近红外技术在制药过程监测和质量控制中的最新应用。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-05-25 DOI: 10.1007/s44211-025-00794-w

Chao Zhang, Yuanyuan Liu, Zhiqiang Tang, Xuejun Chen, Hui Zhang, Nan Liu, Changhong Huo, Zengming Wang, Aiping Zheng

{"title":"Recent applications of near infrared to pharmaceutical process monitoring and quality control.","authors":"Chao Zhang, Yuanyuan Liu, Zhiqiang Tang, Xuejun Chen, Hui Zhang, Nan Liu, Changhong Huo, Zengming Wang, Aiping Zheng","doi":"10.1007/s44211-025-00794-w","DOIUrl":"https://doi.org/10.1007/s44211-025-00794-w","url":null,"abstract":"NIR is a rapid, non-destructive analytical technique that leverages the absorption properties of molecules in the near-infrared region. This technology has become indispensable in the pharmaceutical industry due to its efficiency in analyzing solids, liquids, and various pharmaceutical preparations across numerous applications, including drug discovery, process monitoring, and quality control. With the advent of portable NIR instruments, the technology has gained a pivotal role in PAT, optimizing manufacturing processes and ensuring consistent product quality for real-time product release. This review reports on the remarkable versatility of NIR spectroscopy in the pharmaceutical field, covering a wide range of qualitative determinations, such as sample identification, crystal morphology, and eutectic analysis. It also addresses the quantitative analysis of samples and in-line applications in continuous manufacturing processes, including granulation, drying, tableting, and coating. The fundamentals of NIR spectroscopy, as well as its applications in botanical and biological products, are discussed. Additionally, the review examines the prospects and challenges associated with using NIR spectroscopic techniques. NIR spectroscopy served as an effective and powerful tool for both qualitative and quantitative analysis of pharmaceuticals and for monitoring continuous production.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ion-transfer voltammetry at a supported fluorous liquid membrane | water interface. 负载型含氟液膜|水界面的离子转移伏安法。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-05-23 DOI: 10.1007/s44211-025-00793-x

Kohei Uematsu, Yusuke Iwai, Yuki Nara, Minato Tanaka, Hajime Katano

{"title":"Ion-transfer voltammetry at a supported fluorous liquid membrane | water interface.","authors":"Kohei Uematsu, Yusuke Iwai, Yuki Nara, Minato Tanaka, Hajime Katano","doi":"10.1007/s44211-025-00793-x","DOIUrl":"https://doi.org/10.1007/s44211-025-00793-x","url":null,"abstract":"Ion-transfer reactions at the supported fluorous liquid membrane (SFLM) | water (W) interface were examined using cyclic voltammetry. The SFLM was constructed by impregnating a polytetrafluoroethylene or polyvinylidene fluoride microporous filter with a fluorous solvent, 1H,1H,5H-octafluoropentyl-1,1,2,2-tetrafluoroethyl ether (OFE). Various ions, including tetraphenylarsonium and tetraphenylborate, exhibited nearly reversible voltammetric waves for the ion-transfer reaction at the SFLM | W interface, enabling the determination of formal ion-transfer potentials. The formal Gibbs transfer energies calculated from these potentials were in good agreement with those determined from the conventional OFE | W interface, particularly when the polytetrafluoroethylene membrane was used. This indicates that the fluorophilic property of OFE was maintained in the SFLM. The supported liquid membrane is an effective means of providing a liquid | liquid interface with mechanical stability and ease of handling. Additionally, it enables a marked reduction in the volume of the expensive fluorous solvent required for electrochemical measurements. Therefore, the SFLM system is advantageous for liquid | liquid electrochemical studies and applications using fluorous solvents.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of ene reaction products of Cookson-type fluorescent reagents and their application for recognizing amyloid β aggregation. cookson型荧光试剂烯反应产物的表征及其在β淀粉样蛋白聚集识别中的应用。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-05-23 DOI: 10.1007/s44211-025-00795-9

Kotone Nojiri, Yusuke Hatakawa, Seon Hwa Lee, Tomoyuki Oe

{"title":"Characterization of ene reaction products of Cookson-type fluorescent reagents and their application for recognizing amyloid β aggregation.","authors":"Kotone Nojiri, Yusuke Hatakawa, Seon Hwa Lee, Tomoyuki Oe","doi":"10.1007/s44211-025-00795-9","DOIUrl":"https://doi.org/10.1007/s44211-025-00795-9","url":null,"abstract":"The Cookson reagent (4-phenyl-1,2,4-triazoline-3,5-dione, PTAD) is a powerful dienophile for the Diels-Alder reaction and is used as a protecting group for conjugated dienes in organic chemistry. PTAD also undergoes a selective ene reaction with tyrosine (Tyr) residues in aqueous solvents. In this study, we investigated the ene reaction products with Tyr using two Cookson-type fluorescent reagents, 4-(1-naphthyl)-1,2,4-triazoline-3,5-dione (NTAD) and 4-[2-(6,7-dimethoxy-4-methyl-3-oxo-3,4-dihydroquinoxalyl)ethyl]-1,2,4-triazoline-3,5-dione (DMEQTAD). The fluorescence properties were examined to develop peptide probes using Tyr itself, angiotensin II, and amyloid β40 (Aβ40) as models. NTAD-Tyr showed major changes in fluorescence spectra based on the conditions of the surrounding environment, such as pH, solvent hydrophobicity, and human serum albumin concentration. The increase in the fluorescence intensity of NTAD-Tyr was greater than that of DMEQTAD-Tyr, especially for pH and human serum albumin. Furthermore, a wavelength shift (blue shift) was observed for NTAD-Tyr but not for DMEQTAD-Tyr. Therefore, NTAD was used for the following peptide experiments. NTAD-Aβ40 showed an unexpected red shift in a hydrophobic solvent, which was the opposite shift to NTAD-Tyr and NTAD-Ang II. In the aggregation study of Aβ40 co-incubated with NTAD-Aβ40, the fluorescence of NTAD-Aβ40 increased and reached a plateau earlier than the common thioflavin T method for recognizing β-sheet formation.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature-modulated separation of therapeutic cells, viral vectors, and exosomes using functional polymers. 使用功能聚合物对治疗细胞、病毒载体和外泌体进行温度调节分离。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-05-23 DOI: 10.1007/s44211-025-00785-x

Kenichi Nagase, Hideko Kanazawa

引用次数: 0

Direct insertion of an ion channel immobilized on a soft agarose gel bead into a lipid bilayer: an optimized method. 将固定在软琼脂糖凝胶球上的离子通道直接插入脂质双分子层：一种优化方法。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-05-20 DOI: 10.1007/s44211-025-00792-y

Mami Asakura, Shuyan Wang, Minako Hirano, Toru Ide

{"title":"Direct insertion of an ion channel immobilized on a soft agarose gel bead into a lipid bilayer: an optimized method.","authors":"Mami Asakura, Shuyan Wang, Minako Hirano, Toru Ide","doi":"10.1007/s44211-025-00792-y","DOIUrl":"https://doi.org/10.1007/s44211-025-00792-y","url":null,"abstract":"In this paper, we report the development of a device that improves the conventional artificial lipid bilayer method and can measure channel currents more efficiently. Ion channel proteins are an attractive research target in biophysics, because their functions can be measured at the single-molecule level with high time resolution. In addition, they have attracted attention as targets for drug discovery because of their crucial roles in vivo. Although electrophysiological methods are powerful tools for studying channel proteins, they suffer from low measurement efficiency and require considerable skill. In our previous paper, we reported that by immobilizing channel proteins on agarose gel beads and forming an artificial lipid bilayer on the bead surface, we simultaneously solved two problems that had been hindering the efficiency of the artificial bilayer method: the time-consuming formation of artificial lipid bilayers and the time-consuming incorporation of channels into artificial bilayers. Previous studies have utilized crosslinked hard beads; however, here we show that channel current measurement can be achieved more simply and efficiently using non-crosslinked soft beads. In this study, we detailed the process of immobilizing channel proteins on the surface of non-crosslinked beads through chemical modification, allowing us to measure their channel activity. This method enables current measurements without the need for stringent bead size selection or high negative pressure.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a frontal analysis capillary electrophoresis coupled with time-of-flight mass spectrometry for determining the equilibrium dissociation constant between cyclophilin A and cyclosporin A. 毛细管电泳-飞行时间质谱联用测定亲环素a和环孢素a平衡解离常数的研究进展。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-05-19 DOI: 10.1007/s44211-025-00790-0

Jia Tang, Fan Shui, Li Deng, Yujie Zhang, Qibing Mei, Min Wang, Jing Zeng

{"title":"Development of a frontal analysis capillary electrophoresis coupled with time-of-flight mass spectrometry for determining the equilibrium dissociation constant between cyclophilin A and cyclosporin A.","authors":"Jia Tang, Fan Shui, Li Deng, Yujie Zhang, Qibing Mei, Min Wang, Jing Zeng","doi":"10.1007/s44211-025-00790-0","DOIUrl":"https://doi.org/10.1007/s44211-025-00790-0","url":null,"abstract":"Exploring the interactions between ligands and proteins in vitro and in vivo is of irreplaceable significance for disease prevention, diagnosis, therapeutic development, and drug discovery. Herein, we have developed a novel method for determining the equilibrium dissociation constant (Kd) using frontal analysis capillary electrophoresis coupled with time-of-flight mass spectrometry (FACE-TOF-MS). This method enables the rapid determination of the Kd value for the interaction between cyclophilin A and cyclosporin A. Furthermore, the reliability of this method was validated by comparing it with bio-layer interferometry (BLI). The Kd values determined by FACE-TOF-MS and BLI were 3.32 μM and 0.60 μM, respectively, which fall within an acceptable range when considering previous reports using various methods. This study introduces a novel approach for analyzing molecular interactions, offering potential specificity for simultaneously determining the Kd values of multiple ligands in complex samples, particularly in the areas of biomarker discovery, high-throughput lead compound screening, and therapeutic target validation.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A label-free electrochemical biosensor based on rGO/Ppy nanocomposite for detection of Herpes simplex virus type 1. 基于氧化石墨烯/聚吡啶纳米复合材料的无标记电化学生物传感器检测1型单纯疱疹病毒。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-05-19 DOI: 10.1007/s44211-025-00779-9

Hamed Sadegh Mazji, Fatemeh Zeydi, Amir Hossein Esfandiari, Ehsan Aryan, Seyed Abdolrahim Rezaee, Seyedeh Belin Tavakoly Sany, Zahra Meshkat, Behnaz Hatamluyi

{"title":"A label-free electrochemical biosensor based on rGO/Ppy nanocomposite for detection of Herpes simplex virus type 1.","authors":"Hamed Sadegh Mazji, Fatemeh Zeydi, Amir Hossein Esfandiari, Ehsan Aryan, Seyed Abdolrahim Rezaee, Seyedeh Belin Tavakoly Sany, Zahra Meshkat, Behnaz Hatamluyi","doi":"10.1007/s44211-025-00779-9","DOIUrl":"https://doi.org/10.1007/s44211-025-00779-9","url":null,"abstract":"The herpes simplex virus type 1 (HSV-1) is a prevalent herpes virus found in many regions worldwide, and it infects only humans as its natural hosts. These viruses cause a wide range of acute, latent, and chronic infections in humans, as the virus can cause latent infections as well as diseases such as primary and recurrent oropharyngeal disease, primary and recurrent genital disease, and herpes simplex infection. Therefore, a fast response, cost-effective, sensitive, and selective detection method for HSV-1 is much needed. Here, we present an electrochemical biosensor that is sensitive and selective for the accurate measurement of HSV-1. The glassy carbon electrode was coated with a reduced graphene oxide and polypyrrole nanocomposite (rGO/Ppy-NCs) for modification. The next step involved linking a specific antisense single-stranded DNA oligonucleotide for HSV-1 to the rGO/Ppy-NCs through a π-stacking interaction. The process of DNA hybridization was assessed through the measurement of changes in the free guanine peak current with changing ssDNA target concentrations by employing the method called differential pulse voltammetry (DPV). This biosensor showed a consistent response to synthetic ssDNA target, ranging from 1.0 × 10-15 to 1.0 × 10-11 M, with a detection limit of 0.83 fM. The biosensor's performance was put to the test using a cell culture sample positive for HSV-1, a positive HTLV-1 sample, and a PCR-confirmed negative sample. The obtained results exhibited excellent detection potential of the proposed biosensor as a sensitive and specific biosensing system.","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of baicalin concentration within scutellaria based on terahertz spectroscopy by analyzing aligned principal component. 用对准主成分分析太赫兹光谱预测黄芩中黄芩苷浓度。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2025-05-13 DOI: 10.1007/s44211-025-00786-w

Yizhang Li, Yongbin Guo, Zhongmin Wang, Xiaodi Dong, Guiyun Cao, Xiuewei Yang, Dongyue Han, Zhaoqing Meng, Feng Qi

引用次数: 0