{"title":"Separation and determination of tellurium(IV) and tellurium(VI) using reversed-phase high-performance liquid chromatography-inductively coupled plasma mass spectrometry.","authors":"Tomohiro Narukawa, Ayaka Wada, Chikako Cheong, Koichi Chiba","doi":"10.1007/s44211-024-00663-y","DOIUrl":"https://doi.org/10.1007/s44211-024-00663-y","url":null,"abstract":"<p><p>A new method for speciation analysis of tellurium(IV) and Te(VI) using high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) has been developed. Tellurium(IV) and Te(VI) were completely separated using a reversed-phase column with an L-cysteine eluent under an isocratic elution condition. The detection limits (3σ) of Te(IV) and Te(VI) monitored by HPLC-ICP-MS at m/z 125 were 1.4 and 0.5 ng g<sup>-1</sup>, respectively. The proposed determination method was precisely applied to assessing total concentrations and chemical species of Te in several standard solutions. The recovery rates of Te(IV) and Te(VI) were almost 100% from the results of the addition-recovery examinations, even when a high matrix sample such as seawater was measured. The method was applied to seawater samples and electronic products, and was proved quite effective for environmental risk assessment.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of molecular sensors based on fluorescent proteins for polarized macrophages identification.","authors":"Udari Kalpana Bandaranayake, Hiroki Sato, Miho Suzuki","doi":"10.1007/s44211-024-00649-w","DOIUrl":"https://doi.org/10.1007/s44211-024-00649-w","url":null,"abstract":"<p><p>Macrophages are a type of white blood cells that play key roles in innate immune responses as a part of cellular immunity for host defence and tissue homeostasis. To perform diverse functions, macrophages show high plasticity by transforming to polarized states. They are mainly identified as unpolarized, pro-inflammatory and antiinflammatory states and termed as M0, M1 and M2 macrophages respectively. Discriminating polarized states is important due to strict implication with inflammatory conditions resulting in many diseases as chronic inflammation, neurodegeneration, and cancer etc. Many polarization protein markers have been identified and applied to investigate expression profiles through PCR and other techniques with antibodies. However, they are time and cost consuming and sometimes show insufficient performances. We focused on the mannose receptor (CD206) as representative marker of M2 macrophage recognising terminal mannose. We developed dose dependent mannosylated fluorescent proteins (FPs) by conjugations with mannose derivative for around 20 modifiable sites on FPs surfaces. Maximum modifications did not spoil various features of FPs. We found further sensitive and specific discriminations among M2, M1 and M0 macrophages after treating polarized macrophages with adequately conditioned FPs compared to already established approaches using anti CD206 antibody through flow cytometric analysis. These results might be derived from direct ligand utilizations and increased avidity due to multivalent bindings with abundantly modified multimeric FPs. Our strategy is simple but addresses disadvantages of preceding methods. Moreover, this strategy is applicable to detect other cell surface receptors as FPs can be modified with ligands or recognizable aptamer like molecules.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of reflective fiber-optic surface plasmon resonance sensor for monitoring scale deposition.","authors":"Takuya Okazaki, Miku Yokose, Yudai Ishii, Akira Ueda, Hideki Kuramitz, Tomoaki Watanabe","doi":"10.1007/s44211-024-00653-0","DOIUrl":"https://doi.org/10.1007/s44211-024-00653-0","url":null,"abstract":"<p><p>A reflective surface plasmon resonance (SPR) sensor was evaluated for real-time monitoring of scale deposition. The sensor consists of an optical fiber, only 5 mm at the gold-coated tip of the sensing area. The effect of silica growth on the sensor response was evaluated using a Na<sub>2</sub>SiO<sub>3</sub> solution. The sensitivity of the sensor to silica was 1.6 ± 0.3 nm per one immersion in the solution of 1000 mg/L (as SiO<sub>2</sub>) at 85 °C and subsequnt air drying, as indicated by the SPR peak shift. The amount of silica deposited on the gold surface was measured by the quartz crystal microbalance method, and the SPR sensitivity of 0.089 nm/ng to silica mass was obtained. The detection limit (3σ) of the SPR sensor was 17 ng, corresponding to a thickness of 2.5 nm for amorphous silica. The SPR sensor was tested in geothermal brine sampled at the Sumikawa Geothermal Power Plant, where a clear SPR shift was observed, suggesting the effectiveness of the SPR sensor for scale monitoring.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of the alkyl chain length of α,ω-dichloroalkane on the Gibbs energy of transfer for functional groups.","authors":"Kohei Uematsu, Erina Tanaka, Takanari Tanaka, Hajime Katano","doi":"10.1007/s44211-024-00656-x","DOIUrl":"10.1007/s44211-024-00656-x","url":null,"abstract":"<p><p>Ion-transfer reactions of alkyl and perfluoroalkyl carboxylate ions (CH<sub>3</sub>(CH<sub>2</sub>)<sub>n-2</sub>COO<sup>-</sup> with n = 8-12 and CF<sub>3</sub>(CF<sub>2</sub>)<sub>n-2</sub>COO<sup>-</sup> with n = 3-9) were investigated at the polarized Cl-(CH<sub>2</sub>)<sub>m</sub>-Cl with m = 2, 4, 6, and 8 (O) | water (W) interface to evaluate the effects of n and m on the solvation energy of the ions, as well as on their methylene and terminal groups. These ions exhibited reversible or quasi-reversible voltammetric waves due to their transfer across the O | W interfaces, enabling the determination of formal potentials and formal Gibbs transfer energies from O to W, <math><mrow><mi>Δ</mi> <msubsup><mi>G</mi> <mrow><mrow><mtext>tr,O</mtext> <mo>→</mo> <mtext>W</mtext></mrow> </mrow> <msup><mn>0</mn> <mo>'</mo></msup> </msubsup> </mrow> </math> . The <math><mrow><mi>Δ</mi> <msubsup><mi>G</mi> <mrow><mrow><mtext>tr,O</mtext> <mo>→</mo> <mtext>W</mtext></mrow> </mrow> <msup><mn>0</mn> <mo>'</mo></msup> </msubsup> </mrow> </math> values for CH<sub>3</sub>(CH<sub>2</sub>)<sub>n-2</sub>COO<sup>-</sup> and CF<sub>3</sub>(CF<sub>2</sub>)<sub>n-2</sub>COO<sup>-</sup> increased linearly with n, allowing the estimation of <math><mrow><mi>Δ</mi> <msubsup><mi>G</mi> <mrow><mrow><mtext>tr,O</mtext> <mo>→</mo> <mtext>W</mtext></mrow> </mrow> <msup><mn>0</mn> <mo>'</mo></msup> </msubsup> </mrow> </math> for methylene and difluoromethylene groups, <math><mrow><mi>Δ</mi> <msubsup><mi>G</mi> <mrow><mrow><mtext>tr,O</mtext> <mo>→</mo> <mtext>W</mtext></mrow> </mrow> <msup><mn>0</mn> <mo>'</mo></msup> </msubsup> </mrow> </math> (CH<sub>2</sub>) and <math><mrow><mi>Δ</mi> <msubsup><mi>G</mi> <mrow><mrow><mtext>tr,O</mtext> <mo>→</mo> <mtext>W</mtext></mrow> </mrow> <msup><mn>0</mn> <mo>'</mo></msup> </msubsup> </mrow> </math> (CF<sub>2</sub>), and for their terminal groups, <math><mrow><mi>Δ</mi> <msubsup><mi>G</mi> <mrow><mrow><mtext>tr,O</mtext> <mo>→</mo> <mtext>W</mtext></mrow> </mrow> <msup><mn>0</mn> <mo>'</mo></msup> </msubsup> </mrow> </math> (COO<sup>-</sup> + CH<sub>3</sub>) and <math><mrow><mi>Δ</mi> <msubsup><mi>G</mi> <mrow><mrow><mtext>tr,O</mtext> <mo>→</mo> <mtext>W</mtext></mrow> </mrow> <msup><mn>0</mn> <mo>'</mo></msup> </msubsup> </mrow> </math> (COO<sup>-</sup> + CF<sub>3</sub>). Whereas the <math><mrow><mi>Δ</mi> <msubsup><mi>G</mi> <mrow><mrow><mtext>tr,O</mtext> <mo>→</mo> <mtext>W</mtext></mrow> </mrow> <msup><mn>0</mn> <mo>'</mo></msup> </msubsup> </mrow> </math> (CH<sub>2</sub>) and <math><mrow><mi>Δ</mi> <msubsup><mi>G</mi> <mrow><mrow><mtext>tr,O</mtext> <mo>→</mo> <mtext>W</mtext></mrow> </mrow> <msup><mn>0</mn> <mo>'</mo></msup> </msubsup> </mrow> </math> (CF<sub>2</sub>) hardly changed with the variation in m, the <math><mrow><mi>Δ</mi> <msubsup><mi>G</mi> <mrow><mrow><mtext>tr,O</mtext> <mo>→</mo> <mtext>W</mtext></mrow> </mrow> <msup><mn>0</mn> <mo>'</mo></msup> </msubsup> </mrow> </math> (COO<sup>-</sup> + CH<sub>3</sub>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142118771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Room-temperature mL-to-μL quantitative liquid concentration device for cyclone flow.","authors":"Hidekatsu Tazawa, Kazuma Mawatari","doi":"10.1007/s44211-024-00654-z","DOIUrl":"https://doi.org/10.1007/s44211-024-00654-z","url":null,"abstract":"<p><p>Highly sensitive quantitative analysis of liquids is required in various fields. Analytical instruments and devices such as chromatography, spectroscopic analysis, DNA sequencers, immunoassay, mass spectrometry, and microfluidic devices are utilized for this purpose. Typically, the sample volume is at the milliliter scale, while the analysis volume is at the microliter scale. Consequently, most of the sample is discarded. Therefore, a universal volume interface is required to quantitatively concentrate samples from milliliter to microliter volume. This study introduces a liquid quantitative function to the cyclone concentration method using a millimeter-scale channel, which is highly suitable for controlling liquids at the microliter scale due to its high fluidic resistance against cyclone flow. This method enables the effective control of liquid concentration by cyclone flow. The optimum channel structure is investigated, and a 33-fold concentration of aqueous solutions is demonstrated. Finally, the concentration device is applied to measure molybdenum ions in a river.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploring the synchronized effect of MWCNT/X-manganate (X-Cu, Zn) nanocomposite for the sensitive and selective electrochemical detection of Hg(II) and Pb(II) in water.","authors":"Xingpu Qi, Ping Liu, Fang Yao, Mengli Zhao, Xuanyu Shen, Zhengyun Wang","doi":"10.1007/s44211-024-00652-1","DOIUrl":"https://doi.org/10.1007/s44211-024-00652-1","url":null,"abstract":"<p><p>The presence of heavy metal ions in the environment is a long-lasting problem that requires the simultaneous detection of Hg(II) and Pb(II) which is both vital and challenging. This present study examines a simplified and effective approach for synthesizing multi-walled carbon nanotube-copper manganese oxide (MWCNT-CuMn<sub>2</sub>O<sub>4</sub>) and multi-walled carbon nanotube-zinc manganese oxide (MWCNT-ZnMn<sub>2</sub>O<sub>4</sub>) nanocomposites for electrochemical detection of heavy metal ions. The nanocomposites MWCNT-CuMn<sub>2</sub>O<sub>4</sub> and MWCNT-ZnMn<sub>2</sub>O<sub>4</sub> exceptional electrochemical performance was evaluated using Square Wave Anodic Stripping Voltammetry (SWASV). The fabricated MWCNT-ZnMn<sub>2</sub>O<sub>4</sub> demonstrated lower values of Electrochemical Impedance Spectroscopy (EIS) with charge transfer resistance (R<sub>ct</sub>) of approximately 34.13 Ω. Remarkably, the MWCNT-ZnMn<sub>2</sub>O<sub>4</sub> electrochemical sensor exhibited the widest linear ranges of 0.5-10 μM with sensitive detection limits (0.011 μM for Hg(II) and 0.014 μM for Pb(II)). Interestingly, the MWCNT-ZnMn<sub>2</sub>O<sub>4</sub> sensor showed excellent capability in detecting Hg(II) and Pb(II) in real water samples with a recovery percentage of 94.1% and 91.3%. Overall, the MWCNT-ZnMn<sub>2</sub>O<sub>4</sub> modified GCE showcased superior selectivity, sensitivity, reproducibility, stability, and repeatability.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative study of machine-and deep-learning based classification algorithms for biomedical Raman spectroscopy (RS): case study of RS based pathogenic microbe identification.","authors":"Sisi Guo, Ruoyu Zhang, Tao Wang, Jianfeng Wang","doi":"10.1007/s44211-024-00645-0","DOIUrl":"10.1007/s44211-024-00645-0","url":null,"abstract":"<p><p>One key aspect pushing the frontiers of biomedical RS is dedicated machine- or deep- learning (ML or DL) algorithms. Yet, systematic comparative study between ML and DL algorithms has not been conducted for biomedical RS, largely due to the limited availability of open-source and large Raman spectra dataset. Therefore we compared typical ML partial least square-discriminant analysis (PLS-DA) and DL one dimensional convolution neural network (1D-CNN) based pathogenic microbe identification on 12,000 Raman spectra from six species of microbe (i.e., K. aerogenes (Klebsiella aerogenes), C. albicans (Candida albicans), C. glabrata (Candida glabrata), Group A Strep. (Group A Streptococcus), E. coli1 (Escherichia coli1), E. coli2 (Escherichia coli2)) when 100%, 75%, 50% and 25% of the 12,000 Raman spectra were retained. The total Raman dataset was analyzed with 80% split for training and 20% for testing. The 100% retained testing dataset accuracy, area under curve (AUC) of the receiver operating characteristic (ROC) curve were 95.25% and 0.997 for 1D-CNN, which are higher than those (89.42% and 0.979) of PLS-DA. Yet, PLS-DA outperforms 1D-CNN for 75%, 50% and 25% retained testing dataset. The resultant accuracies and AUCs demonstrated the performance reliance of PLS-DA and 1D-CNN on Raman spectra number. Besides, both loadings on the latent variables of PLS-DA and the saliency maps of 1D-CNN largely captured Raman peaks arising from DNA and proteins with comparable interpretability. The results of the current work indicated that both ML and DL algorithms should be explored for application-wise Raman spectra identification to select whichever with higher accuracies and AUCs.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Aptamer regulated peroxidase-like activity of cobalt oxyhydroxide nanosheets for colorimetric detection of kanamycin.","authors":"Shuyan Xiao, Jiafeng Wu, Mingqin Kang, Zhongping Dong","doi":"10.1007/s44211-024-00655-y","DOIUrl":"https://doi.org/10.1007/s44211-024-00655-y","url":null,"abstract":"<p><p>A straightforward label-free colorimetric aptasensor utilizing the aptamer-enhanced peroxidase-like activity of cobalt oxyhydroxide (CoOOH) nanosheets has been established for kanamycin detection. In the kanamycin-free state, aptamers adsorb onto the CoOOH surface through electrostatic forces, enhancing the peroxidase-like activity of CoOOH and thereby resulting in a strong absorption signal and a yellow hue in 3,3',5,5'-tetramethylbenzidine (TMB) upon termination of the reaction with a stop solution. Conversely, upon the introduction of kanamycin, aptamers and CoOOH nanosheets compete for binding to kanamycin, resulting in a significant decrease in the number of aptamers bound to CoOOH. As a result, the activity of CoOOH diminishes, leading to a corresponding reduction in coloration and absorbance of the solution. Hence, the quantitative determination of kanamycin could be realized by analyzing the absorbance variations. Under optimal conditions, the aptasensor demonstrated high sensitivity and specificity, with a linear detection range from 500 nM to 5 µM and a detection limit as low as 54.6 nM. Moreover, the aptasensor effectively identified kanamycin in river water samples, achieving a recovery rate between 91.7% and 102.1%. This approach offers good practicability and provides a novel platform for kanamycin detection in environmental samples.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142078907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Doaa A. Abdel-Kader, Adel M. Kamal Eldean, Mohamed M. Abd El-Wahab, Mohamed S. Galal, Elham Y. Hashem
{"title":"Removal of ceftriaxone and cefotaxime antibiotics from industrial wastewater via complexation with Cu(II)","authors":"Doaa A. Abdel-Kader, Adel M. Kamal Eldean, Mohamed M. Abd El-Wahab, Mohamed S. Galal, Elham Y. Hashem","doi":"10.1007/s44211-024-00632-5","DOIUrl":"10.1007/s44211-024-00632-5","url":null,"abstract":"<div><p>A high-performance liquid chromatographic method (HPLC) with UV detection is described for determination of ceftriaxone sodium (CFX) and cefotaxime sodium (CFM) content in pharmaceutical industrial wastewater. These methods are based on the detection of these antibiotics via the formation of chelate complexes with Cu(II). The developed Liquid Chromatographic method offers symmetric peak shape, good resolution and reasonable retention time for both drugs. The removal percentage reached about 100 and 92.1% at pH 7.2 for CFX and CFM, respectively. In UV detection, the removal of the chelating antibiotics were based on forming of chelate complexes with Cu(II) which detected at λ<sub>max<b> =</b></sub> 253 and 244 nm for CFX and CFM, respectively. Linearity, accuracy and precision were found to be acceptable over the concentration range of 5.99–59.86 µg mL<sup>−1</sup> for CFX and 14.33–71.63 µg mL<sup>−1</sup> for CFM. The proposed method can be used for the quality control of industrial wastewater containing CFX and CFM.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"40 11","pages":"1997 - 2004"},"PeriodicalIF":1.8,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-024-00632-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}