Analytical Sciences最新文献_第5页

Squaraine dye as a fluorescent probe for highly sensitive detection of pyrophosphate and alkaline phosphatase Squaraine染料作为荧光探针用于焦磷酸盐和碱性磷酸酶的高灵敏度检测。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-12-26 DOI: 10.1007/s44211-024-00697-2

Wenxuan Zhu, Shuhua Zhao, Bei Yu, Yucun Tao, Chaoyang Wang, Lei Shi, Xiufeng Zhang, Jing Meng, Chengqiu Wu, Chunguang Ding, Lijia Yu

{"title":"Squaraine dye as a fluorescent probe for highly sensitive detection of pyrophosphate and alkaline phosphatase","authors":"Wenxuan Zhu, Shuhua Zhao, Bei Yu, Yucun Tao, Chaoyang Wang, Lei Shi, Xiufeng Zhang, Jing Meng, Chengqiu Wu, Chunguang Ding, Lijia Yu","doi":"10.1007/s44211-024-00697-2","DOIUrl":"10.1007/s44211-024-00697-2","url":null,"abstract":"<div><p>We synthesized a squaraine dye (F-0) to develop a method for detecting pyrophosphate (PPi) and alkaline phosphatase (ALP) by modulating the fluorescence of F-0. The fluorescence intensity of the F-0 system was quenched upon the addition of Cu<sup>2+</sup> ions; however, it was restored when PPi was introduced due to the formation of a complex between PPi and Cu<sup>2+</sup>. Since ALP can hydrolyze PPi, the fluorescence of the system was quenched again upon the addition of ALP. Based on these principles, we established a fluorescent probe that exhibits an \"off–on–off\" fluorescence response. The detection limits of this method for PPi and ALP were 103 nmol dm<sup>−3</sup> and 0.18 U dm<sup>−3</sup>, respectively. Moreover, this method demonstrates good selectivity and specificity and can be applied to the detection of PPi in actual samples.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 3","pages":"289 - 296"},"PeriodicalIF":1.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An optical BOD biosensor based on intracellular ATP measurements in genetically modified Saccharomyces cerevisiae. 基于转基因酿酒酵母胞内ATP测量的光学生化需氧量生物传感器。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-12-23 DOI: 10.1007/s44211-024-00706-4

Yuto Kanno, Yuta Tateno, Takuo Akimoto

{"title":"An optical BOD biosensor based on intracellular ATP measurements in genetically modified Saccharomyces cerevisiae.","authors":"Yuto Kanno, Yuta Tateno, Takuo Akimoto","doi":"10.1007/s44211-024-00706-4","DOIUrl":"https://doi.org/10.1007/s44211-024-00706-4","url":null,"abstract":"<p><p>A biosensor for biochemical oxygen demand (BOD) was developed based on intracellular 5'-adenosine triphosphate (ATP) measurements in Saccharomyces cerevisiae. Intracellular ATP was measured using an engineered protein named ATeam, comprising a bacterial F<sub>0</sub>F<sub>1</sub>-ATP synthase ε subunit sandwiched between cyan fluorescent protein and mVenus, a modified yellow fluorescent protein. Because the binding of ATP to ATeam induces changes in the fluorescence spectra owing to Fӧrster resonance energy transfer, S. cerevisiae expressing ATeam is expected to show spectral changes owing to the intracellular ATP produced by the metabolism of the BOD sample. A glycogen phosphorylase knockout S. cerevisiae strain expressing ATeam was prepared, and the fluorescence spectra of the strain were analyzed. Changes in the fluorescence spectra of glucose in the medium were observed, which exhibited a linear relationship with the glucose concentration (0-100 mg/L, R<sup>2</sup> = 0.970). Responses to lactose, fructose, sucrose, Glu, Asp, His, and Gly were evaluated and compared with typical BOD measurements. The results of this comparison suggest that a BOD biosensor based on intracellular ATP can be used for BOD measurements. A BOD standard solution comprising glucose and glutamic acid (GGA) was calibrated across a concentration range of 0 to 100 mg/L. Finally, simulated real samples were prepared using real pond water and GGA was measured. The correlation between the BOD value evaluated using intracellular ATP and that evaluated using the 5-day BOD test showed a linear relationship with R<sup>2</sup> = 0.944.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitivity-enhanced potentiometric measurement by incorporating graphitic carbon nitride into the ion-to-electron transducer of potassium ion-selective electrodes. 通过将石墨氮化碳纳入钾离子选择电极的离子-电子换能器的灵敏度增强电位测量。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-12-20 DOI: 10.1007/s44211-024-00705-5

Mingpeng Yang, Jinghan Cheng, Xingqiang Zhao, Jun Cai, Wangping Zhou

{"title":"Sensitivity-enhanced potentiometric measurement by incorporating graphitic carbon nitride into the ion-to-electron transducer of potassium ion-selective electrodes.","authors":"Mingpeng Yang, Jinghan Cheng, Xingqiang Zhao, Jun Cai, Wangping Zhou","doi":"10.1007/s44211-024-00705-5","DOIUrl":"https://doi.org/10.1007/s44211-024-00705-5","url":null,"abstract":"<p><p>In recent years, wearable sweat sensors have garnered significant attention for real-time monitoring of human physiological information because of their ability to continuously and non-invasively detect multiple sweat biomarkers. Among these, potentiometric sensors stand out for their low power consumption, low cost, compact design, and real-time monitoring capabilities, making them an ideal alternative for sweat analysis. However, enhancing the sensitivity of ion-selective electrodes (ISEs), a critical parameter of potentiometric sensors, remains a challenging research focus. In this work, the sensitivity of K<sup>+</sup> ISEs was significantly enhanced by doping two-dimensional nanoparticles graphitic carbon nitride (g-C₃N₄) into the ion-to-electron transducer of the electrode via electrodeposition. The calibration curve slope of the K<sup>+</sup> potentiometric sensors with doped g-C<sub>3</sub>N<sub>4</sub> reached 59.6 mV/dec, representing a 33% increase in sensitivity compared to the control sensor without g-C₃N₄. Furthermore, the developed sensors demonstrated excellent repeatability, and anti-interference capabilities. Finally, the feasibility of the prepared sensors was further validated in artificial sweat. The large specific surface area of g-C₃N₄ combined with the excellent conductivity of PEDOT: PSS, significantly improved the sensitivity of ISEs in this study. This innovative approach paves a new avenue for the application of two-dimensional materials in potentiometric sensors, potentially advancing the field of real-time sweat analysis.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142863106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient single-channel current measurements of the human BK channel using a liposome-immobilized gold probe. 利用脂质体固定金探针对人体BK通道进行有效的单通道电流测量。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-12-20 DOI: 10.1007/s44211-024-00707-3

Minako Hirano, Mami Asakura, Toru Ide

{"title":"Efficient single-channel current measurements of the human BK channel using a liposome-immobilized gold probe.","authors":"Minako Hirano, Mami Asakura, Toru Ide","doi":"10.1007/s44211-024-00707-3","DOIUrl":"https://doi.org/10.1007/s44211-024-00707-3","url":null,"abstract":"<p><p>The human BK channel (hBK) is an essential membrane protein that regulates various biological functions, and its dysfunction leads to serious diseases. Understanding the biophysical properties of hBK channels is crucial for drug development. Artificial lipid bilayer recording is used to measure biophysical properties at the single-channel level. However, this technique is time-consuming and complicated; thus, its measurement efficiency is very low. Previously, we developed a novel technique to improve the measurement efficiency by rapidly forming lipid bilayer membranes and incorporating ion channels into the membrane using a hydrophilically modified gold probe. To further improve our technique for application to the hBK channel, we combined it using the gold probe with a liposome fusion method. Using a probe on which liposomes containing hBK channels were immobilized, the channels were efficiently incorporated into the lipid bilayer membrane, and the measured channel currents showed the current characteristics of the hBK channel. This technique will be useful for the efficient measurements of the channel properties of hBK and other biologically important channels.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic labeling of RNA using ribonucleoside analogs enables the evaluation of RNA synthesis and degradation rates. 利用核糖核苷类似物对RNA进行代谢标记，可以评估RNA的合成和降解率。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-12-19 DOI: 10.1007/s44211-024-00704-6

Hidenori Tani

引用次数: 0

Bromine isotope splitting in vibrational spectra of bromoform by time-resolved transient transmission spectroscopy 时间分辨瞬态透射光谱法研究溴仿振动光谱中溴同位素的分裂。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-12-16 DOI: 10.1007/s44211-024-00702-8

W. Gadomski, K. Polok, K. Skała, B. Ratajska-Gadomska

{"title":"Bromine isotope splitting in vibrational spectra of bromoform by time-resolved transient transmission spectroscopy","authors":"W. Gadomski, K. Polok, K. Skała, B. Ratajska-Gadomska","doi":"10.1007/s44211-024-00702-8","DOIUrl":"10.1007/s44211-024-00702-8","url":null,"abstract":"<div><p>The femtosecond pump–probe technique, i.e. the transient transmission spectroscopy, has been used for the first time, to detect the vibrational spectra of symmetric fundamentals ν<sub>2</sub> and ν<sub>3</sub> in bromoform and chloroform. The spectra were obtained by fast Fourier transforms of the time domain signals. For both, CHCl<sub>3</sub> and CHBr<sub>3</sub>, there are four isotopologues contributing to the spectra, due to the existence of two stable isotopes; chlorine, <sup>35</sup>Cl and <sup>37</sup>Cl, and bromine, <sup>79</sup>Br and <sup>81</sup>Br, respectively. While for chloroform the isotope splitting of the ν<sub>3</sub> spectral band can be observed even in the spontaneous Raman scattering, for bromoform it is not detectable. Herewith we show that using the time domain spectroscopy and the windowed Fourier transform method we can provide the high resolution spectrum of the ν<sub>3</sub> fundamental in bromoform, in which the contributions of all isotopologues are well distinguishable. The data have been collected for few volume concentrations of the studied liquids diluted in the neutral solvent CCl<sub>4</sub>. It is shown that the intensity pattern of the spectra evolves with decreasing concentration and for the ν<sub>3</sub> fundamental it reaches the natural abundance pattern at a very high dilution. The simple theoretical model, which treats the molecules in a liquid as interacting oscillators, allows us to explain the dependence of the shape of the spectrum on the strength of the intermolecular interactions.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 3","pages":"211 - 224"},"PeriodicalIF":1.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyper-Raman spectroscopy of non-proteinogenic amino acids 非蛋白质氨基酸的超拉曼光谱。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-12-13 DOI: 10.1007/s44211-024-00698-1

Tsung-Han Liu, Masanari Okuno

{"title":"Hyper-Raman spectroscopy of non-proteinogenic amino acids","authors":"Tsung-Han Liu, Masanari Okuno","doi":"10.1007/s44211-024-00698-1","DOIUrl":"10.1007/s44211-024-00698-1","url":null,"abstract":"<div><p>We report 532-nm and 1064-nm excited hyper-Raman (HR) spectra of representative non-proteinogenic amino acids, including α-, β-, and γ-amino acids. Different from the common 20 proteinogenic amino acids, natural non-proteinogenic amino acids cannot be incorporated into proteins during translation, while they are indispensable as intermediates in many processes like biosynthesis and neurotransmitters. In 532-nm excited HR spectra, the COO<sup>─</sup> symmetric stretching bands are commonly intense, and the NH<sub>3</sub><sup>+</sup> bands are clearly observable. In addition, based on the reported IR and Raman study, we found that some HR bands are IR-active but Raman-inactive. In contrast, HR signals with the 1064-nm excitation are much weaker than the 532-nm excitation. Nevertheless, we observed the COO<sup>─</sup> scissoring band unexpectedly, much stronger than other bands with the 1064-nm excitation. Our results suggest that the electronic resonance effect plays a role in enabling us to detect HR signals in the UV region readily. We expect that this study provides a supplementary reference for HR spectroscopy of natural amino acids.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 3","pages":"201 - 210"},"PeriodicalIF":1.8,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-024-00698-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extension of the molar absorption coefficient for non-ideal mixtures: an application to aqueous monovalent alcohol solutions. 非理想混合物摩尔吸收系数的扩展：一价醇水溶液的应用。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-12-13 DOI: 10.1007/s44211-024-00703-7

Akifumi Ikehata

{"title":"Extension of the molar absorption coefficient for non-ideal mixtures: an application to aqueous monovalent alcohol solutions.","authors":"Akifumi Ikehata","doi":"10.1007/s44211-024-00703-7","DOIUrl":"https://doi.org/10.1007/s44211-024-00703-7","url":null,"abstract":"<p><p>The hydration state of the alcohols was investigated using the extended molar absorption coefficient, which redefines the molar absorption coefficient as a differential coefficient of concentration. The extended molar absorption coefficient is a function of the concentration calculated from the difference in absorbance, and is consistent with the conventional molar absorption coefficient, allowing a complete quantitative comparison. The quantitative performance was verified using IR and NIR absorption spectra of aqueous solutions of monovalent alcohols (methanol, ethanol, 1-propanol, 2-propanol, and tert-butanol) that were soluble in water at any mixing ratio. Extended molar absorption coefficient spectra were calculated for the combination bands of water, which were further separated by multivariate curve resolution-alternating least squares (MCR-ALS) into molecular species with different peak wavenumbers: strongly hydrogen-bonded (SHB), weakly hydrogen-bonded (WHB), and free OH species. The number of water species that change when one alcohol molecule increases, i.e., the perturbed hydration number (PHN), was calculated by comparison with the conventional molar absorption coefficient of pure water. The calculated PHN indicates that the numbers of SHB and WHB species are reversed at approximately 20 wt%, and that the free OH species increase at higher alcohol concentrations and are more pronounced for alcohols with bulky alkyl groups. These results provide a quantitative answer to the long-debated question of anomalies in water-alcohol mixing.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic SERS spectral analysis based on data augmentation and attention mechanism strategy 基于数据增强和注意机制策略的抗生素SERS谱分析。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-12-11 DOI: 10.1007/s44211-024-00695-4

Hang Zhao, Min Zhou, Chunlin Liu, Hongheng Sun, Panshuo Zhang, Jun Ma, Xiaofeng Shi

{"title":"Antibiotic SERS spectral analysis based on data augmentation and attention mechanism strategy","authors":"Hang Zhao, Min Zhou, Chunlin Liu, Hongheng Sun, Panshuo Zhang, Jun Ma, Xiaofeng Shi","doi":"10.1007/s44211-024-00695-4","DOIUrl":"10.1007/s44211-024-00695-4","url":null,"abstract":"<div><p>The analysis of Raman spectrum data has gradually transitioned into the era of machine learning. However, it is still constrained by the challenge of acquiring large volumes of raw data and the issue of losing characteristic information from spectral data. In this paper, we propose a strategy that combines data amplification and attention mechanisms for analyzing antibiotic spectral data. Firstly, a Generative Adversarial Network was employed to amplify the SERS spectrum of eight antibiotics by 10 times, to augment the dataset to fulfill the requirements of the neural network. Then, the amplified data is input into a one-dimensional convolutional neural network with an attentional mechanism module, which enables a more accurate capture of spectral feature information. The one-dimensional convolutional neural network achieved a 97.5% accuracy in classifying eight antibiotics. The accuracy of the four mixtures within the same class was 89.4%.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 3","pages":"251 - 261"},"PeriodicalIF":1.8,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoionization of pyrenemethylamine-labeled oligosaccharides: a new MALDI-TOF precursor ion-type for efficient fragmentation 芘甲胺标记低聚糖的光电离：一种新的MALDI-TOF前体离子型高效碎片化。

IF 1.8 4区化学

Analytical Sciences Pub Date : 2024-12-10 DOI: 10.1007/s44211-024-00700-w

Andreas H. Franz, Nathan Ta, Ella Nguyen, Kendall Bromley, Hana Rosenblatt, Aprilrose Fabro, Geoff Lin-Cereghino

{"title":"Photoionization of pyrenemethylamine-labeled oligosaccharides: a new MALDI-TOF precursor ion-type for efficient fragmentation","authors":"Andreas H. Franz, Nathan Ta, Ella Nguyen, Kendall Bromley, Hana Rosenblatt, Aprilrose Fabro, Geoff Lin-Cereghino","doi":"10.1007/s44211-024-00700-w","DOIUrl":"10.1007/s44211-024-00700-w","url":null,"abstract":"<div><p>Oligosaccharides were covalently labeled with 1-pyrenemethylamine (1-PMA) in 15 min at 75 °C with high yield, separated by quantitative HPLC in less than 20 min, and characterized by off-line Matrix-Assisted Laser Desorption/Ionization (MALDI) Time-of-Flight (TOF) mass spectrometry (MS). A new MALDI mass spectral precursor ion for fragmentation studies was observed from 2,5-dihydroxybenzoic acid matrix (DHB, hydroquinone/benzoquinone redox system) through photo-induced reductive elimination. The resulting high-energy protonated glycosylamine provided excellent fragmentation efficiency (Y-, B-ions, and combination losses), with ions covering almost the entire structure of several oligosaccharides at the low picomol level. With the new method, glycans from commercially available standard glycoproteins and glycans from bioengineered β-lactoglobulin expressed in the <i>bgs13</i> mutant of <i>Pichia pastoris</i> were analyzed.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 3","pages":"297 - 310"},"PeriodicalIF":1.8,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0