Analytical Sciences最新文献_第5页

Multimodal machine learning for integrating heterogeneous analytical systems 集成异构分析系统的多模态机器学习。

IF 2 4区化学

Analytical Sciences Pub Date : 2026-06-15 DOI: 10.1007/s44211-026-00942-w

Shun Muroga, Hideaki Nakajima, Taiyo Shimizu, Kazufumi Kobashi, Kenji Hata

{"title":"Multimodal machine learning for integrating heterogeneous analytical systems","authors":"Shun Muroga, Hideaki Nakajima, Taiyo Shimizu, Kazufumi Kobashi, Kenji Hata","doi":"10.1007/s44211-026-00942-w","DOIUrl":"10.1007/s44211-026-00942-w","url":null,"abstract":"<div><p>Understanding structure–property relationships in complex materials requires integrating complementary measurements across multiple length scales. Here we propose an interpretable “multimodal” machine learning framework that unifies heterogeneous analytical systems for end-to-end characterization, demonstrated on carbon nanotube (CNT) films whose properties are highly sensitive to microstructural variations. Quantitative morphology descriptors are extracted from SEM images via binarization, skeletonization, and network analysis, capturing curvature, orientation, intersection density, and void geometry. These SEM-derived features are fused with Raman indicators of crystallinity/defect states, specific surface area from gas adsorption, and electrical surface resistivity. Multi-dimensional visualization using radar plots and UMAP reveals clear clustering of CNT films according to crystallinity and entanglements. Regression models trained on the multimodal feature set show that nonlinear approaches, particularly XGBoost, achieve the best predictive accuracy under leave-one-out cross-validation. Feature-importance analysis further provides physically meaningful interpretations: surface resistivity is primarily governed by junction-to-junction transport length scales, crystallinity/defect-related metrics, and network connectivity, whereas specific surface area is dominated by intersection density and void size. The proposed multimodal machine learning framework offers a proof-of-concept demonstration for data-driven and explainable characterization of complex materials using multimodal machine learning.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 7","pages":"635 - 643"},"PeriodicalIF":2.0,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-026-00942-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous detection of multiple sdhB SNPs in Botrytis cinerea using a universal quenching probe–based LAMP assay 基于通用淬火探针的LAMP法同时检测灰葡萄孢中多个sdhB snp

IF 2 4区化学

Analytical Sciences Pub Date : 2026-06-15 DOI: 10.1007/s44211-026-00938-6

Shiro Fukuta, Hiroko Kojima-Ando, Ken-ichiro Mori, Tomoaki Hara, Masami Tsuzuki, Reina Inagaki, Kenta Tsunekawa, Yuta Uchida, Mizuho Arakawa, Kensuke Yamada

{"title":"Simultaneous detection of multiple sdhB SNPs in Botrytis cinerea using a universal quenching probe–based LAMP assay","authors":"Shiro Fukuta, Hiroko Kojima-Ando, Ken-ichiro Mori, Tomoaki Hara, Masami Tsuzuki, Reina Inagaki, Kenta Tsunekawa, Yuta Uchida, Mizuho Arakawa, Kensuke Yamada","doi":"10.1007/s44211-026-00938-6","DOIUrl":"10.1007/s44211-026-00938-6","url":null,"abstract":"<div><p>Succinate dehydrogenase inhibitors (SDHIs) are widely used to control <i>Botrytis cinerea</i>, but resistance caused by multiple point mutations in the <i>sdh</i> genes has become a major concern. To enable rapid and accurate identification of SDHI‑resistant genotypes, we developed a loop‑mediated isothermal amplification (LAMP) assay using a universal quenching probe (UQProbe LAMP) capable of simultaneously detecting multiple single nucleotide polymorphisms (SNPs) within the same target region. Using a single UQProbe and a common LAMP primer set, the method successfully distinguished four <i>sdh</i>B genotypes—wild type, H272R, H272Y, and H272V—based on characteristic melting temperatures (approximately 56 °C, 50 °C, 44.5 °C, and 38.5 °C, respectively). This single‑probe, multi‑SNP detection strategy eliminates the need for mutation‑specific primers or multiple probes, providing a simple and efficient workflow for SNP typing. The UQProbe LAMP system developed in this study offers a rapid, cost‑effective, and versatile platform for multiplex SNP discrimination, applicable not only to fungicide resistance monitoring but also to a wide range of genetic diagnostic applications requiring precise SNP detection.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><img></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 9","pages":"887 - 893"},"PeriodicalIF":2.0,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148248996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconstruction of molecular vibrational spectra from light–molecular vibration coupling spectra using deep learning 基于深度学习的光-分子振动耦合谱重建分子振动谱。

IF 2 4区化学

Analytical Sciences Pub Date : 2026-06-11 DOI: 10.1007/s44211-026-00933-x

Yoshiaki Nishijima, Saoki Imai, Haruto Maeda

{"title":"Reconstruction of molecular vibrational spectra from light–molecular vibration coupling spectra using deep learning","authors":"Yoshiaki Nishijima, Saoki Imai, Haruto Maeda","doi":"10.1007/s44211-026-00933-x","DOIUrl":"10.1007/s44211-026-00933-x","url":null,"abstract":"<div><p>Light–molecular vibration coupling on metasurfaces induces complex optical phenomena, such as Fano resonance and Rabi splitting, which complicate molecular identification via conventional spectroscopic analysis. In this study, we developed an analytical system that uses deep feature learning to directly extract molecule-specific absorption information from these complex spectra. For a molecular vibration model with a single peak, we evaluated a DenseNet-169 convolutional neural network (CNN) model using 1,496 spectra generated via finite-difference time-domain (FDTD) simulations; however, the results were suboptimal. In contrast, for a two-peak molecular vibration model, we trained multiple CNN models, including DenseNet-169, on a dataset of 80,267 spectra. Consequently, we successfully reconstructed absorption coefficients with an extremely high accuracy, achieving a mean coefficient of determination (<span>(R^2)</span>) of 0.9209, even in complex systems with overlapping vibrational peaks. This approach demonstrates significant potential as a foundational technology for next-generation molecular sensing.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 7","pages":"619 - 624"},"PeriodicalIF":2.0,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-026-00933-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148216130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring Cd2+ and Pb2+ in natural water with an electrochemical flow cell using three-electrode system 用三电极电化学流动电池监测天然水中的Cd2+和Pb2+。

IF 2 4区化学

Analytical Sciences Pub Date : 2026-06-09 DOI: 10.1007/s44211-026-00936-8

Huizhen Huang, Zhiwei Lai

{"title":"Monitoring Cd2+ and Pb2+ in natural water with an electrochemical flow cell using three-electrode system","authors":"Huizhen Huang, Zhiwei Lai","doi":"10.1007/s44211-026-00936-8","DOIUrl":"10.1007/s44211-026-00936-8","url":null,"abstract":"<div><p>A highly advanced three-electrode system integrated with an electrochemical flow cell has been meticulously developed for the precise determination of heavy metal ions in water, utilizing a mercury-modified electrode through differential pulse anodic stripping voltammetry. The electrochemical flow cell has been ingeniously designed to effectively replace conventional and outdated three-electrode systems. By employing this innovative system in conjunction with the mercury-modified electrode, a strikingly low limit of detection (0.31 µg L<sup>− 1</sup> for Cd<sup>2+</sup> and 0.75 µg L<sup>− 1</sup> for Pb<sup>2+</sup>) and an impressively wide range for determination (0–60 µg L<sup>− 1</sup> for Cd<sup>2+</sup> and 0–100 µg L<sup>− 1</sup> for Pb<sup>2+</sup>) have been successfully achieved, fully meeting the stringent requirements for drinking water analysis. A relative standard deviation (RSD) of 5.34% for Cd<sup>2+</sup> and 2.66% for Pb<sup>2+</sup> proving the precision of the method. This system realizes real-time monitoring of Cd<sup>2+</sup> and Pb<sup>2+</sup> in natural water, featuring high accuracy and efficiency. Finally, the anti-shaking test convincingly demonstrated that the system has great potential for on-site monitoring, thus opening up a new path for water quality assessment.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 9","pages":"847 - 853"},"PeriodicalIF":2.0,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A general framework for extrapolation-aware prediction reliability in forward and inverse analyses of Gaussian mixture regression models 高斯混合回归模型正逆分析中外推感知预测可靠性的一般框架。

IF 2 4区化学

Analytical Sciences Pub Date : 2026-06-01 DOI: 10.1007/s44211-026-00924-y

Hiromasa Kaneko

{"title":"A general framework for extrapolation-aware prediction reliability in forward and inverse analyses of Gaussian mixture regression models","authors":"Hiromasa Kaneko","doi":"10.1007/s44211-026-00924-y","DOIUrl":"10.1007/s44211-026-00924-y","url":null,"abstract":"<div><p>Gaussian mixture regression (GMR) and direct inverse analysis (DIA) are powerful tools for molecular, material, and process design, but their reliability decreases when extrapolation occurs beyond the training data. To address this challenge, an index of extrapolation (IoE) that evaluates extrapolation potential and prediction trustworthiness in both forward and inverse analyses of GMR models is proposed. Defined as the negative logarithm of the probability density function, the IoE enables stable identification of interpolation-like and extrapolation-like regions. Validation with numerical simulations confirmed its ability to distinguish these regions, while applications to data sets of organic solubility, inorganic superconductivity, and batch processes demonstrated that high-IoE regions correspond to reduced prediction reliability, where prediction errors may show larger dispersion. The proposed IoE provides a practical and generalizable framework to assess applicability domains, enhancing the interpretability and reliability of machine learning models. This advancement strengthens data-driven design and supports more efficient discovery of novel molecules, materials, and processes.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><img></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 7","pages":"609 - 617"},"PeriodicalIF":2.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-026-00924-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148136603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First implementation of a polymer–polymer aqueous two-phase system to improve lateral-flow immunoassay sensitivity via dextranase integration on paper 首次实现聚合物-聚合物水两相系统，通过纸面上的葡聚糖酶整合来提高横向流动免疫测定的灵敏度。

IF 2 4区化学

Analytical Sciences Pub Date : 2026-05-29 DOI: 10.1007/s44211-026-00925-x

Frances D. Nicklen, Christina H. Hadeed, Fiona Zhang, Alyssa Pama, Daniel T. Kamei

{"title":"First implementation of a polymer–polymer aqueous two-phase system to improve lateral-flow immunoassay sensitivity via dextranase integration on paper","authors":"Frances D. Nicklen, Christina H. Hadeed, Fiona Zhang, Alyssa Pama, Daniel T. Kamei","doi":"10.1007/s44211-026-00925-x","DOIUrl":"10.1007/s44211-026-00925-x","url":null,"abstract":"<div><p>Lateral-flow immunoassays (LFAs) are commonly used to detect proteins in bodily fluids, providing rapid and accessible patient sample testing at the point of care (POC). Although commercially successful, LFAs suffer from low sensitivity. To improve their sensitivity, our group was the first to implement polymer-salt and micellar aqueous two-phase systems (ATPSs) to preconcentrate various targets into a smaller volume prior to LFA application. For small hydrophilic protein targets that distribute fairly evenly in polymer-salt and micellar ATPSs, polymer–polymer ATPSs may be used for preconcentration instead. A poly(ethylene glycol)-dextran ATPS can preconcentrate proteins into the dextran-rich bottom phase, but has not been integrated with LFAs due to poor sample flow caused by the highly viscous dextran-rich phase. To address this limitation, we implemented the enzyme dextranase (DN) to degrade dextran in the bottom phase sample, reducing its viscosity before LFA application. Enzymatic studies were performed to investigate various conditions, which were then further evaluated with imbibition studies to demonstrate improved fluid flow on paper. Subsequently, the ATPS was combined with DN to achieve a tenfold improvement in the limit of detection (LOD) for the protein transferrin spiked in artificial saliva when compared to a conventional LFA setup. To reduce liquid handling steps and thus improve POC accessibility, DN was dehydrated on paper and applied to the LFA to achieve a similar tenfold improvement in sensitivity. By integrating DN, this study represents the first successful use of a polymer–polymer ATPS to improve LFA sensitivity, and introduces the possibility of integrating a different class of ATPSs with the LFA.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 8","pages":"745 - 758"},"PeriodicalIF":2.0,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148052073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced peroxidase-like activity of L-glutamic acid-coated Fe3O4 quantum dots for colorimetric detection of L-cysteine in serum and milk l -谷氨酸包被Fe3O4量子点增强过氧化物酶样活性比色法检测血清和牛奶中的l -半胱氨酸。

IF 2 4区化学

Analytical Sciences Pub Date : 2026-05-28 DOI: 10.1007/s44211-026-00931-z

Shipei Cheng, Jinhua Liang, Guidan Huang, Hongying Ye, Mengdan Yang, Bingjian Lin, Jinfang Nie, Wenyuan Zhu, Yun Zhang

{"title":"Enhanced peroxidase-like activity of L-glutamic acid-coated Fe3O4 quantum dots for colorimetric detection of L-cysteine in serum and milk","authors":"Shipei Cheng, Jinhua Liang, Guidan Huang, Hongying Ye, Mengdan Yang, Bingjian Lin, Jinfang Nie, Wenyuan Zhu, Yun Zhang","doi":"10.1007/s44211-026-00931-z","DOIUrl":"10.1007/s44211-026-00931-z","url":null,"abstract":"<div><p>A novel colorimetric sensing platform was developed for rapid detection of L-cysteine (L-Cys) in human serum and milk samples, utilizing L-glutamic acid-coated Fe<sub>3</sub>O<sub>4</sub> quantum dots (Glu-Fe<sub>3</sub>O<sub>4</sub> QDs) with enhanced peroxidase-like activity. The Glu-Fe<sub>3</sub>O<sub>4</sub> QDs were synthesized via a two-step hydrothermal method, where L-glutamic acid modification significantly improved both catalytic performance and stability compared to unmodified Fe<sub>3</sub>O<sub>4</sub> nanoparticles. Characterization by TEM, XPS, and FTIR confirmed the ultrasmall size (2.57 ± 0.78 nm) and successful surface functionalization with carboxyl/amino groups. The nanozymes catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H<sub>2</sub>O<sub>2</sub>, generating a blue color change measurable at 652 nm. L-Cys suppressed this reaction by competitively consuming hydroxyl radicals, enabling quantitative detection with a linear range of 5–50 µM (R<sup>2</sup> = 0.997) and a low detection limit of 0.73 µM. The method exhibited high selectivity against interfering amino acids and ions, and achieved recoveries of 96.99–107.59% in spiked serum and milk samples with RSDs < 3.86%. This work provides a simple, cost-effective tool for monitoring L-Cys in complex biological matrices, with potential applications in food safety and clinical diagnostics.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div><div><p>Ultrasmall L-glutamic acid-coated Fe3O4 quantum dots (Glu-Fe3O4 QDs) were synthesized, exhibiting enhanced peroxidase-like activity that catalyzes TMB-H2O2 chromogenic reaction. The system enables rapid colorimetric detection of L-cysteine in human serum and milk with high selectivity and a detection limit of 0.73 μM</p></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 8","pages":"779 - 785"},"PeriodicalIF":2.0,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148040812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sensitive photoelectrochemical biosensor based on DepAu/TiO2 and T-Hg2+-T structure 基于DepAu/TiO2和T-Hg2+-T结构的灵敏光电化学生物传感器。

IF 2 4区化学

Analytical Sciences Pub Date : 2026-05-28 DOI: 10.1007/s44211-026-00929-7

Mengjie Li, Li Gong, Yuanbo Ding, Yiwen He

{"title":"A sensitive photoelectrochemical biosensor based on DepAu/TiO2 and T-Hg2+-T structure","authors":"Mengjie Li, Li Gong, Yuanbo Ding, Yiwen He","doi":"10.1007/s44211-026-00929-7","DOIUrl":"10.1007/s44211-026-00929-7","url":null,"abstract":"<div><p>Herein, a sensitive photoelectrochemical (PEC) biosensor was designed for the detection of mercury ion (Hg<sup>2+</sup>) based on deposited gold nanoparticles (DepAu) enhanced titanium dioxide (TiO<sub>2</sub>) accompanied by thymine-Hg<sup>2+</sup>-thymine (T-Hg<sup>2+</sup>-T) structure. TiO<sub>2</sub>-modified glassy carbon electrode (GCE) exhibited a baseline anodic photocurrent, which was substantially enhanced upon electrochemical deposition of Au NPs. This PEC signal amplification was ascribed to the dual contributions of the improved photon utilization and the facilitated charge transfer. The electrode was then subjected to stepwise modification with T-rich DNA followed by 1-hexanethiol (HT), each incremental layer engendering a concomitant decrement in the photocurrent response. Ultimately, the introduction of target Hg<sup>2+</sup> enabled specific coordination with the T-rich DNA sequence, facilitating the formation of a stable T-Hg<sup>2+</sup>-T complex architecture on the GCE surface. This coordination event substantially amplified interfacial steric hindrance, thereby impeding electron transfer and effectuating pronounced photocurrent suppression. The quenched PEC response provided a quantitative metric for Hg<sup>2+</sup> determination. The sensitive and selective detection of Hg<sup>2+</sup> was achieved in the range of 100 pmol/L to 100 µmol/L, with the detection limit of 33.3 pmol/L. This PEC biosensing platform holds considerable promise for extending to the detection of diverse heavy metal ions and other environmental contaminants by rational design of recognition elements.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 8","pages":"769 - 778"},"PeriodicalIF":2.0,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148040770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence quenching-based methods for pharmaceutical analysis: a comprehensive review of principles, probes, and applications 基于荧光猝灭的药物分析方法：原理、探针和应用的综合综述。

IF 2 4区化学

Analytical Sciences Pub Date : 2026-05-27 DOI: 10.1007/s44211-026-00923-z

Meriem Dadouch, Teffaha Fergoug, Youcef Bouhadda, Derouicha Matmour

{"title":"Fluorescence quenching-based methods for pharmaceutical analysis: a comprehensive review of principles, probes, and applications","authors":"Meriem Dadouch, Teffaha Fergoug, Youcef Bouhadda, Derouicha Matmour","doi":"10.1007/s44211-026-00923-z","DOIUrl":"10.1007/s44211-026-00923-z","url":null,"abstract":"<p>The fluorescence quenching or turn-off strategy is an emerging method for determination of fluorescent and non-fluorescent active principal ingredients (API). It is gaining a growing interest in advanced pharmaceutical applications due to its high sensitivity and selectivity. The present review represents a comprehensive and up-to-date overview of the quantitative determination of drugs by fluorescence quenching-based methods with a special focus on probes for the first time. The theoretical fundamentals of fluorescence quenching, including static and dynamic quenching as well as the inner filter effect are presented. Subsequently, the key analytical and methodological considerations of fluorescence quenching-based methods, including analytical parameters (probe, solvent, pH), instrumentation, analytical performance with emphasis on application of green and white analytical chemistry principles, are highlighted. Finally, the properties of fluorescent probes (organic molecules, inorganic elements, salts, complexes, enzyme-catalyzed products, nanomaterials, nanocomposites, and hybrid materials) and their analytical approaches for drug determination are systematically appraised and summarized with relevant analytical conditions and parameters of the methods. The review also highlighted the advances and challenges of fluorescence quenching-based analytical methods.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 8","pages":"657 - 675"},"PeriodicalIF":2.0,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148025680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small-molecule-based CUT&RUN for G-quadruplex DNA using a cyclic naphthalene diimide–copper complex 使用环二亚胺-铜络合物的基于小分子的g -四重体DNA的CUT&RUN。

IF 2 4区化学

Analytical Sciences Pub Date : 2026-05-26 DOI: 10.1007/s44211-026-00928-8

Yukina Sanada, Satoshi Fujii, Shigeori Takenaka, Shinobu Sato

{"title":"Small-molecule-based CUT&RUN for G-quadruplex DNA using a cyclic naphthalene diimide–copper complex","authors":"Yukina Sanada, Satoshi Fujii, Shigeori Takenaka, Shinobu Sato","doi":"10.1007/s44211-026-00928-8","DOIUrl":"10.1007/s44211-026-00928-8","url":null,"abstract":"<div><p>G-quadruplex (G4) DNA structures are widely distributed in biologically important genomic regions and are involved in gene regulation and genome stability. However, experimental identification of G4 sites in chromatin remains challenging. Here, we report a multifunctional small-molecule ligand, cNDI-CuGGHE, that integrates selective G4 recognition and copper-mediated DNA cleavage within a single molecular scaffold. Spectroscopic analysis showed that cNDI-CuGGHE preferentially binds to various G4 structures through terminal stacking on the G-quartet plane with binding constants on the order of 10⁶ M⁻¹ and significantly stabilizes G4 DNA compared with duplex DNA. Gel electrophoresis confirmed efficient and structure-dependent DNA cleavage in the presence of sodium ascorbate and hydrogen peroxide. Genomic DNA recovered from a cNDI-CuGGHE-based CUT&RUN-like assay in HeLa cells was analyzed by quantitative PCR using primers flanking the G4-forming regions of the c<i>-kit</i> and c-<i>myc</i> promoters. The reduced amplification of G4-spanning regions was consistent with preferential DNA cleavage at G4 sites in chromatin. These results demonstrate the feasibility of using small-molecule ligands as alternatives to antibody–enzyme conjugates for G4-targeted chromatin analysis.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 8","pages":"759 - 767"},"PeriodicalIF":2.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-026-00928-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148025695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0