Analytical Sciences最新文献

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SERS detection of uranyl based on MOF-coated gold nanooctahedron hybrid. 基于 MOF 涂层金纳米八面体杂化物的 SERS 检测铀酰。
IF 1.8 4区 化学
Analytical Sciences Pub Date : 2024-08-24 DOI: 10.1007/s44211-024-00646-z
Chao Yuan, Hongwei Ge, Benmei Cao, Suhua Wang
{"title":"SERS detection of uranyl based on MOF-coated gold nanooctahedron hybrid.","authors":"Chao Yuan, Hongwei Ge, Benmei Cao, Suhua Wang","doi":"10.1007/s44211-024-00646-z","DOIUrl":"https://doi.org/10.1007/s44211-024-00646-z","url":null,"abstract":"<p><p>The ability to sensitively and quantitatively detect uranyl in complex samples plays a vital role in environmental monitoring. In this work, an MOF-coated gold (Au) nanohybrid was synthesized for uranyl detection by surface-enhanced Raman scattering (SERS) technology. The MOF shell not only prevents the Au nanoparticles from rapid aggregation, but also effectively enhances the Raman signal of uranyl. A detection limit of as low as 0.5 μM could be achieved in solution, which could be comparable to the previously reported ones from SERS-based approaches. Moreover, the prepared SERS-active substrate was also applied to uranyl detection in real samples.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: New stability indicating RP-HPLC methods for the determination of related substances and assay of trametinib acetic acid: a mass balance approach 更正:用于测定相关物质和检测曲美替尼乙酸的新型稳定性指示 RP-HPLC 方法:质量平衡法。
IF 1.8 4区 化学
Analytical Sciences Pub Date : 2024-08-21 DOI: 10.1007/s44211-024-00650-3
G. Himabindu, Y. Satyanarayana Reddy, A. V. S. S. Prasad, C. Ramadas, Hemant Kumar Sharma
{"title":"Correction: New stability indicating RP-HPLC methods for the determination of related substances and assay of trametinib acetic acid: a mass balance approach","authors":"G. Himabindu,&nbsp;Y. Satyanarayana Reddy,&nbsp;A. V. S. S. Prasad,&nbsp;C. Ramadas,&nbsp;Hemant Kumar Sharma","doi":"10.1007/s44211-024-00650-3","DOIUrl":"10.1007/s44211-024-00650-3","url":null,"abstract":"","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"40 11","pages":"2097 - 2097"},"PeriodicalIF":1.8,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Combination of on-line sample preconcentration by large-volume dual preconcentration by isotachophoresis and stacking (LDIS) with field-amplified sample injection (FASI) on Y-channel microchips. 在 Y 型通道微芯片上将大容量异向电泳和堆积双重预浓缩(LDIS)在线样品预浓缩与现场放大样品进样(FASI)相结合。
IF 1.8 4区 化学
Analytical Sciences Pub Date : 2024-08-20 DOI: 10.1007/s44211-024-00647-y
Fumihiko Kitagawa, Sora Sato, Tomohiro Suzuki, Takayuki Kawai
{"title":"Combination of on-line sample preconcentration by large-volume dual preconcentration by isotachophoresis and stacking (LDIS) with field-amplified sample injection (FASI) on Y-channel microchips.","authors":"Fumihiko Kitagawa, Sora Sato, Tomohiro Suzuki, Takayuki Kawai","doi":"10.1007/s44211-024-00647-y","DOIUrl":"https://doi.org/10.1007/s44211-024-00647-y","url":null,"abstract":"<p><p>In our previous study, the combination of two on-line sample preconcentration techniques, large-volume sample stacking with an electroosmotic flow (EOF) pump (LVSEP) and transient isotachophoresis (tITP), in microchip electrophoresis (MCE) was developed, which was named large-volume dual preconcentration by isotachophoresis and stacking (LDIS). LDIS was apparently effective for improving the sensitivity and the peak shape. In LDIS, however, there was a limit to the improvement of the sensitivity enhancement factor (SEF) since the amount of analytes to be concentrated was limited to the channel volume. To overcome this issue, in the present article, LDIS was coupled with field-amplified sample injection (FASI) technique on Y-shaped channel microchips. The use of a Y-channel in LDIS-FASI allowed consecutive LVSEP, FASI and tITP enrichments with a simple voltage control. In conventional LVSEP and LDIS analyses of a standard analyte, the SEFs were evaluated to be 2630 and 13,100, respectively, whereas in LDIS-FASI that was increased to 27,900 even at the FASI injection time of 0 s. To achieve higher SEFs, furthermore, the FASI injection time was increased to 150 s, resulting in the best SEF of 58,500. It should be emphasized that the peak width in LDIS-FASI was quite narrow, only 0.3-3.1 s, while in normal LVSEP that was 13 s. Furthermore, the LDIS-FASI technique was applied to the analysis of oligosaccharide mixture. Due to the focusing effect by LDIS-FASI, the resolutions were improved from 0.97-1.57 to 2.08-2.73.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enzyme-aided amplification strategy for sensitive detection of methamphetamine based on fluorescence aptamer sensor. 基于荧光适配体传感器灵敏检测甲基苯丙胺的酶辅助放大策略。
IF 1.8 4区 化学
Analytical Sciences Pub Date : 2024-08-16 DOI: 10.1007/s44211-024-00648-x
Zheyu Wang, Yandan Wang, Yishuo Tong, Weifen Niu
{"title":"Enzyme-aided amplification strategy for sensitive detection of methamphetamine based on fluorescence aptamer sensor.","authors":"Zheyu Wang, Yandan Wang, Yishuo Tong, Weifen Niu","doi":"10.1007/s44211-024-00648-x","DOIUrl":"https://doi.org/10.1007/s44211-024-00648-x","url":null,"abstract":"<p><p>Methamphetamine (METH) abuse poses a serious risk to human health and social stability. It is critical to develop sensitive and selective methods for detecting METH. Here, we develop a fluorescence aptamer sensor to detect METH based on DNA exonuclease III (Exo III), graphene oxide (GO), and FAM-labeled aptamer. First, the sensor used GO's strong binding capacity to adsorb and quench the fluorescence of the aptamer attached to GO surface. When METH was added to the system, the formation of stable complex for aptamer and METH dissociated from the surface of GO, leading to a fluorescence restoration. Then, the fluorescence signal was further amplified by using Exo III to liberate target METH for cyclic hybridization. And the gel electrophoresis experiment further verified the reliability of this strategy. This aptamer sensor exhibited a low detection limit (0.52 nM) and excellent selectivity under optimal conditions. Notably, this sensor has been successfully validated in the detection of METH in urine and saliva samples, exhibited commendable recovery (94.00-104.65%). Its benefits include facile, sensitive, and rapid. Expected to be used in practical METH detection.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Separation of iodate, bromide, nitrite, nitrate, and iodide in seawater by ion chromatography using 1-aminoundecyl group chemically bonded silica columns 利用 1-aminoundecyl 基团化学键合硅胶柱,通过离子色谱法分离海水中的碘酸根、溴化物、亚硝酸盐、硝酸盐和碘化物。
IF 1.8 4区 化学
Analytical Sciences Pub Date : 2024-08-14 DOI: 10.1007/s44211-024-00639-y
Kazuaki Ito, Michinari Noguchi, Yuuta Horioka, Joji Ohshita, Takeshi Hirokawa
{"title":"Separation of iodate, bromide, nitrite, nitrate, and iodide in seawater by ion chromatography using 1-aminoundecyl group chemically bonded silica columns","authors":"Kazuaki Ito,&nbsp;Michinari Noguchi,&nbsp;Yuuta Horioka,&nbsp;Joji Ohshita,&nbsp;Takeshi Hirokawa","doi":"10.1007/s44211-024-00639-y","DOIUrl":"10.1007/s44211-024-00639-y","url":null,"abstract":"<div><p>The separation and detection of six common inorganic anions (iodate (IO<sub>3</sub><sup>−</sup>), bromate (BrO<sub>3</sub><sup>−</sup>), bromide (Br<sup>−</sup>), nitrite (NO<sub>2</sub><sup>−</sup>), nitrate (NO<sub>3</sub><sup>−</sup>), and iodide (I<sup>−</sup>)) in pure water and 35 ‰ artificial seawater were examined by ion chromatography (IC). As packing materials of separation columns, 1-aminoundecyl group chemically bonded silica (AUS) gels were prepared. Separation of the anions in pure water was achieved using separation columns (150 mm × 4.6 mm i.d.) packed with the AUS gels, 0.1 M NaCl + 5 mM phosphate buffer (pH 4.5) as eluent, and a UV detector (wavelength 225 nm). The anions in artificial seawater were separated and detected with a 300 mm-long column without interferences by matrix anions such as chloride (Cl<sup>−</sup>) and sulfate (SO<sub>4</sub><sup>2−</sup>). The stationary phases have high-capacity anion-exchange/hydrophilic/hydrophobic interaction mixed-modes. The IC system was applied to five inorganic anions, IO<sub>3</sub><sup>−</sup>, Br<sup>−</sup>, NO<sub>2</sub><sup>−</sup>, NO<sub>3</sub><sup>−</sup>, and I<sup>−</sup> in seawater of the Seto-Inland Sea, Japan. The detection limits (DLs, <i>S</i>/<i>N</i> = 3) were 11 µg L<sup>−1</sup> (IO<sub>3</sub><sup>−</sup>), 93 (Br<sup>−</sup>), 1.3 (NO<sub>2</sub><sup>−</sup>), 1.4 (NO<sub>3</sub><sup>−</sup>), and 1.1 (I<sup>−</sup>) for a 100-µL sample injection.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"40 11","pages":"2027 - 2032"},"PeriodicalIF":1.8,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-024-00639-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141981532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comprehensive spatial distribution profiling of bioactive emodin in mouse organs using mass spectrometry imaging 利用质谱成像技术全面分析生物活性大黄素在小鼠器官中的空间分布。
IF 1.8 4区 化学
Analytical Sciences Pub Date : 2024-08-13 DOI: 10.1007/s44211-024-00644-1
Si-Qi Han, Xiao-Kang Fu, Wei Ha, Yan-Ping Shi
{"title":"Comprehensive spatial distribution profiling of bioactive emodin in mouse organs using mass spectrometry imaging","authors":"Si-Qi Han,&nbsp;Xiao-Kang Fu,&nbsp;Wei Ha,&nbsp;Yan-Ping Shi","doi":"10.1007/s44211-024-00644-1","DOIUrl":"10.1007/s44211-024-00644-1","url":null,"abstract":"<div><p>Emodin is an important anthraquinone compound with good anti-inflammatory activity in Chinese traditional medicine rhubarb. Detailed spatial distribution information in bio-tissues plays an important role in revealing the pharmacodynamics, toxicology and chemical mechanism of emodin. Herein, the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF-MSI) analytical method was established to obtain information on the spatial and temporal changes of emodin in multiple mouse tissue sections (heart, liver, spleen, lung, kidney, and brain) after intraperitoneal injection of emodin in mice. The measurements were accomplished in the negative ion mode in the range of <i>m/z</i> 250–285 Da with a spatial resolution on 40 µm. It was found that emodin was predominantly distributed in the arteriolar vascular region of the heart, the capsule region of the spleen, and the cortex of the kidney. Moreover, the MALDI-TOF-MSI result implied that emodin might be distributed in the brain. These more detailed spatial distribution information provides the significant reference for investigating the action mechanism of emodin, which cannot be obtained from conventional LC–MS analysis. The distribution trend of emodin in the results of MALDI-TOF-MSI analysis agreed with the ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) results well, demonstrating the complementarity and reliability of the established MALDI-TOF-MSI method. Our work provided a label-free molecular imaging method to investigate the precise spatial distribution of emodin in various organs, which prove great potential in studying the effective substances and mechanism of rhubarb.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"40 11","pages":"2063 - 2073"},"PeriodicalIF":1.8,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of hydroquinone in beverages using colorimetric and electrochemical sensors on paper-based device 在纸质装置上使用比色法和电化学传感器测定饮料中的对苯二酚。
IF 1.8 4区 化学
Analytical Sciences Pub Date : 2024-08-10 DOI: 10.1007/s44211-024-00638-z
Chanya Punthama, Chim Math, Wijitar Dungchai
{"title":"Determination of hydroquinone in beverages using colorimetric and electrochemical sensors on paper-based device","authors":"Chanya Punthama,&nbsp;Chim Math,&nbsp;Wijitar Dungchai","doi":"10.1007/s44211-024-00638-z","DOIUrl":"10.1007/s44211-024-00638-z","url":null,"abstract":"<div><p>Hydroquinone (HQ) is a phenolic compound used in industry processes. We aim to demonstrate a rapid and simple procedure for the determination of HQ. This work has developed two techniques, including colorimetric and electrochemical sensors on paper-based devices. Firstly, we have developed the colorimetric detection for the rapid screening test of HQ using 1.5% 4-(dimethylamino) benzaldehyde with alkaline condition (5 M NaOH). Under suitable conditions, the calibration curve between the intensity and HQ concentration was in the range of 50–500 mg L<sup>−1</sup>. Then, we developed a multi-walled carbon nanotube/graphene oxide/copper/palladium/platinum (MWCNT/GO/Cu/Pd/Pt) onto a screen-printed carbon electrode (SPCE). The optimal amount of MWCNT/GO/Cu/Pd/Pt nanomaterial is 2 mg for HQ detection. The linear concentration range was found in the range 1 to 20 mg L<sup>−1</sup> and a detection limit was found to be 0.40 mg L<sup>−1</sup> (3.6 µM) for HQ. Moreover, the proposed device can be applied to determine HQ in real samples and is inexpensive technique, portable, and low consumer time.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"40 11","pages":"2033 - 2041"},"PeriodicalIF":1.8,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141911371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of hydrophobic modification on biocompatibility of Alaska pollock gelatin microparticles 疏水改性对阿拉斯加狭鳕明胶微粒生物相容性的影响。
IF 1.8 4区 化学
Analytical Sciences Pub Date : 2024-08-09 DOI: 10.1007/s44211-024-00643-2
Ying Chuin Yee, Takeshi Mori, Shima Ito, Tetsushi Taguchi, Yoshiki Katayama
{"title":"Impact of hydrophobic modification on biocompatibility of Alaska pollock gelatin microparticles","authors":"Ying Chuin Yee,&nbsp;Takeshi Mori,&nbsp;Shima Ito,&nbsp;Tetsushi Taguchi,&nbsp;Yoshiki Katayama","doi":"10.1007/s44211-024-00643-2","DOIUrl":"10.1007/s44211-024-00643-2","url":null,"abstract":"<div><p>This study investigates the impact of hydrophobic modification on the immunogenicity, cytotoxicity, and inflammatory response of Alaska pollock gelatin (ApGltn) microparticles (MPs). Gelatin, known for its inherent biocompatibility, was modified with decyl group (C10) to explore potential alterations in its interaction with the immune system. Immunogenicity was evaluated through the measurement of material-specific IgM and IgG responses, indicating no significant increase post-modification. Cytotoxicity against Caco-2 cell lines and NF-κB-mediated LPS-induced inflammation were also assessed, revealing no exacerbation by the modified MPs. Furthermore, C10 modification with different types of linkage such as secondary amine and amide structure did not influence immune reactivity. These findings suggest that C10 modification maintains the non-immunogenicity and biocompatibility of gelatin MPs, supporting their potential use in biomedical applications.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"40 11","pages":"2053 - 2061"},"PeriodicalIF":1.8,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sustainable approaches to analyzing phenolic compounds: a green chemistry perspective 分析酚类化合物的可持续方法:绿色化学视角。
IF 1.8 4区 化学
Analytical Sciences Pub Date : 2024-08-06 DOI: 10.1007/s44211-024-00640-5
Rahul Makhija, Pallavi Barik, Ashish Mehta, Subrahmanya S. Ganti, Vivek Asati
{"title":"Sustainable approaches to analyzing phenolic compounds: a green chemistry perspective","authors":"Rahul Makhija,&nbsp;Pallavi Barik,&nbsp;Ashish Mehta,&nbsp;Subrahmanya S. Ganti,&nbsp;Vivek Asati","doi":"10.1007/s44211-024-00640-5","DOIUrl":"10.1007/s44211-024-00640-5","url":null,"abstract":"<div><p>Innovative and eco-friendly methodologies for the determination of phenolic compounds, showing a paradigm shift in analytical chemistry toward sustainability. Phenolic compounds, valued for their diverse health benefits, have historically been analyzed using methods that often involve hazardous solvents and energy-intensive processes. This review focuses on green analytical chemistry principles, emphasizing sustainability, reduced environmental impact, and analytical efficiency. The use of DES, specifically Ch: Chl-based DES, emerges as a prominent green alternative for extracting phenolic compounds from various sources. The integration of UAE with DES enhances extraction efficiency, contributing to a more sustainable analytical approach. Furthermore, the review highlights the significance of DLLME and SPME in reducing solvent consumption and simplifying extraction procedures. These techniques exemplify the commitment to making phenolic compound analysis environmentally friendly. The incorporation of portable measurement tools, such as smartphones, into analytical methodologies is a notable aspect discussed in the review. Techniques like UA-DLLME leverage portable devices, making phenolic compound determination more accessible and versatile. Anticipating the future, the review foresees ongoing advancements in sustainable analytical approaches, driven by collaborative efforts across diverse disciplines. Novel solvents, extraction techniques, and portable measurement methods are expected to play pivotal roles in the continuous evolution of green analytical methodologies for the analysis of phenolic compounds. The review encapsulates a transformative journey toward environmentally responsible and efficient analytical practices, paving the way for further research and application in diverse analytical settings.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"40 11","pages":"1947 - 1968"},"PeriodicalIF":1.8,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141896561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Design of deep-red emissive forced intercalation-induced light-up peptide as an indicator for the HIV-1 TAR RNA-ligand assay: integration of benzo[c,d]indole-quinoline (BIQ) cyanine dye into Tat peptide 设计深红色发射性强制插层诱导发光肽作为 HIV-1 TAR RNA 配体检测的指示剂:将苯并[c,d]吲哚喹啉(BIQ)氰胺染料整合到 Tat 肽中。
IF 1.8 4区 化学
Analytical Sciences Pub Date : 2024-08-05 DOI: 10.1007/s44211-024-00642-3
Akunna Francess Ujuagu, Yusuke Sato, En Ting Tabitha Lee, Seiichi Nishizawa
{"title":"Design of deep-red emissive forced intercalation-induced light-up peptide as an indicator for the HIV-1 TAR RNA-ligand assay: integration of benzo[c,d]indole-quinoline (BIQ) cyanine dye into Tat peptide","authors":"Akunna Francess Ujuagu,&nbsp;Yusuke Sato,&nbsp;En Ting Tabitha Lee,&nbsp;Seiichi Nishizawa","doi":"10.1007/s44211-024-00642-3","DOIUrl":"10.1007/s44211-024-00642-3","url":null,"abstract":"<div><p>We report on a deep-red emissive fluorogenic peptide probe for human immunodeficiency virus-1 (HIV-1) trans-activation responsive (TAR) RNA as an indicator for fluorescence indicator displacement (FID) assay. The probe design is based on the concept of the forced intercalation of thiazole orange (TO) dyes (FIT) on the peptide backbone, as recently proposed by our group, where the Q (glutamic acid) residue in the Tat peptide (RKKRR-Q-RRR) is replaced with TO as if it were an amino acid surrogate. Here, instead of green emissive TO, we utilized a deep-red emissive benzo[<i>c</i>,<i>d</i>]indole-quinoline (BIQ) cyanine dye developed previously by our group for imaging of nucleolar RNA in living cells. The developed 9-mer FIT peptide (RKKRR-BIQ-RRR; named BIQ-FiLuP) exhibits a significant off–on signaling ability for TAR RNA (λ<sub>em</sub> = 660 nm, <i>I</i>/<i>I</i><sub>0</sub> = 130-fold, Φ<sub>free</sub> = 0.0009, Φ<sub>bound</sub> = 0.052), and the dissociation constant <i>K</i><sub>d</sub> reaches ca. 1 nM. When used in FID assay, BIQ-FiLuP, like TO-based FiLuP, is able to distinguish between competitive and noncompetitive inhibitors, which has never been demonstrated with all previous indicators for TAR RNA. Deep-red emissive BIQ-FiLuP facilitates the evaluation of green to yellow emissive ligands without suffering from optical interference. The combination use with green emissive TO-based FiLuP (λ<sub>em</sub> = 541 nm) would cover the examination of a wide range of fluorescent test compounds.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"40 11","pages":"2089 - 2095"},"PeriodicalIF":1.8,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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