Analytical Sciences最新文献_第8页

Advances in microfluidic chip technology for cell analysis 用于细胞分析的微流控芯片技术进展。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-09-30 DOI: 10.1007/s44211-025-00851-4

Luxi Shu, Yan Zhang

{"title":"Advances in microfluidic chip technology for cell analysis","authors":"Luxi Shu, Yan Zhang","doi":"10.1007/s44211-025-00851-4","DOIUrl":"10.1007/s44211-025-00851-4","url":null,"abstract":"<div><p>Microfluidic chips play a crucial role in the field of cell analysis. These miniature chips integrate seamlessly across key cell analysis stages, including sample preparation, cell culture, sorting, lysis, and detection. They possess many advantages such as miniaturization, integration, automation, and portability. The size of their microchannels is comparable to that of cells, thus enabling research to be conducted at the single-cell or even subcellular organelle level. They can also simulate the physiological conditions in vivo, conduct non-destructive or minimally destructive detections, and meet the requirements of high-throughput cell analysis, which is beneficial for parallel operations and continuous analysis. This article reviews the technological progress of microfluidic chips, focusing on three major directions: cell sorting and enrichment, single-cell analysis, and dynamic microenvironment simulation. The article also analyzes the challenges faced by this technology, such as cell damage control, handling of multicellular heterogeneity, data interpretation, etc., and proposes coping strategies such as the development of new biomaterials, multimodal integration technology, and artificial intelligence assistance. By combining cutting-edge technologies such as nanotechnology, 3D printing, and organoid culture, the functions of microfluidic chips can be further expanded to enable the simulation and analysis of more complex biological systems and provide important technical support for cell biology research and clinical translation.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 12","pages":"1829 - 1851"},"PeriodicalIF":2.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Recent topics in X-ray absorption spectroscopy: integrating theory, instrumentation, and applications x射线吸收光谱学的最新课题：集成理论、仪器和应用

IF 2 4区化学

Analytical Sciences Pub Date : 2025-09-26 DOI: 10.1007/s44211-025-00840-7

Daitaro Ishikawa

引用次数: 0

Rolling circle transcription-based fluorescent aptasensor for highly sensitive and onsite detection of microcystin-LR 基于滚动环转录的荧光适体传感器，用于高灵敏度和现场检测微囊藻毒素。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-09-19 DOI: 10.1007/s44211-025-00839-0

Shuran Yang, Yuling Xu, Cheng Zhang, Ke Xu, Zhiyong Hu, Zhiqiang Xia

{"title":"Rolling circle transcription-based fluorescent aptasensor for highly sensitive and onsite detection of microcystin-LR","authors":"Shuran Yang, Yuling Xu, Cheng Zhang, Ke Xu, Zhiyong Hu, Zhiqiang Xia","doi":"10.1007/s44211-025-00839-0","DOIUrl":"10.1007/s44211-025-00839-0","url":null,"abstract":"<div><p>Onsite and sensitive detection of microcystin-LR (MC-LR) is crucial for monitoring environmental pollution and protecting human health, particularly in the context of increasing cyanobacterial harmful algal blooms (CyanoHABs). Herein, this study reports a rolling circle transcription (RCT)-based fluorescent aptasensor designed for rapid and quantitative onsite detection of MC-LR in real water samples. Aptamers specific to MC-LR were immobilized onto magnetic beads (MBs) and hybridized with blocker complementary DNA strands to form the MB aptasensor complex. Upon recognition and binding of MC-LR molecules in water samples, the blocker DNA strands were displaced and subsequently cyclized into circular DNA structures via T4 DNA ligase-mediated ligation. These circular DNA templates initiated RCT, generating abundant fluorescent Mango RNA reporters. The resulting fluorescent signals exhibited a robust positive correlation with MC-LR concentration, with a detection limit of 39 pM and a linear range from 0.01 to 3000 nM, demonstrating high sensitivity and specificity. The aptasensor showed excellent specificity against structural analogs and common interferents. Recovery tests in Haihe River samples yielded rates between 98.33% and 101.17%, with relative standard deviations below 5%. This sensing platform offers a simple operational procedure, minimal sample preparation, and excellent applicability for real environmental water monitoring. Thus, the developed RCT-based aptasensor provides a promising, cost-effective alternative for onsite MC-LR detection and contributes significantly to environmental safety and public health management.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 11","pages":"1779 - 1790"},"PeriodicalIF":2.0,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A new approach for determining trace platinum concentrations in macroalgae and evaluating tissue-specific distribution 测定大型藻类中痕量铂浓度和评估组织特异性分布的新方法。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-09-18 DOI: 10.1007/s44211-025-00849-y

Ziwei Li, Asami S. Mashio, Kuo H. Wong, Hiroshi Hasegawa

{"title":"A new approach for determining trace platinum concentrations in macroalgae and evaluating tissue-specific distribution","authors":"Ziwei Li, Asami S. Mashio, Kuo H. Wong, Hiroshi Hasegawa","doi":"10.1007/s44211-025-00849-y","DOIUrl":"10.1007/s44211-025-00849-y","url":null,"abstract":"<div><p>Platinum (Pt), extensively used in automotive catalytic converters and anti-cancer drugs, has led to elevated concentrations in the environment. Monitoring Pt levels in coastal ecosystems is crucial as excessive accumulation may cause ecotoxicological impacts. In this study, <i>Undaria pinnatifida</i>, a common edible macroalgae in Japan known for its high affinity for platinum group elements, was evaluated as a biomonitor for monitoring Pt concentrations and assessing its anthropogenic influences in coastal areas. An analytical protocol using inductively coupled plasma mass spectrometry (ICP-MS) was developed and optimized for Pt quantification in macroalgal tissues. The optimal digestion conditions included 0.24 mL/g of hydrofluoric acid and the addition of 7 mL/g of hydrochloric acid, which effectively reduced white precipitate formation and improved Pt recovery. The established method demonstrated excellent sensitivity, achieving a method blank of 16 ppt and a detection limit of 5 ppt, surpassing previously reported approaches. Applying this optimized protocol, tissue-specific Pt concentrations were determined in different parts of <i>U. pinnatifida</i> collected from various coastal zones. The results revealed significant spatial variations in Pt concentrations among plant organs: concentrations in blades, stipes, and sporophylls decreased from the outer bay to the inner bay, while holdfasts concentrations exhibited the opposite trend. These findings suggest distinct Pt accumulation behaviors between above-ground and below-ground tissues of <i>U. pinnatifida</i>, underscoring its potential for monitoring spatial variations of Pt in coastal environments.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 12","pages":"1875 - 1882"},"PeriodicalIF":2.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statistical quantification of SERS signals in microfluidic flow using AuNP-bound polystyrene microparticles 利用aunp结合聚苯乙烯微粒统计量化微流态中SERS信号。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-09-09 DOI: 10.1007/s44211-025-00846-1

Shu Mingcong, Akihisa Miyagawa, Shoji Ishizaka

{"title":"Statistical quantification of SERS signals in microfluidic flow using AuNP-bound polystyrene microparticles","authors":"Shu Mingcong, Akihisa Miyagawa, Shoji Ishizaka","doi":"10.1007/s44211-025-00846-1","DOIUrl":"10.1007/s44211-025-00846-1","url":null,"abstract":"<div><p>Surface-enhanced Raman scattering (SERS) is a powerful analytical technique; however, its quantitative application has been limited by the instability of substrates and significant signal fluctuations. In this study, we demonstrated that 4-aminobenzenethiol (4-ATP) can be quantitatively detected through statistical analysis of SERS signal intensity distributions obtained using citrate-stabilized AuNPs, biotin-functionalized AuNPs, and gold nanoparticle (AuNP)-bound polystyrene (PS) microparticles. Raman spectra obtained in bulk aqueous solution under static conditions showed that the detection sensitivity of 4-ATP using AuNP-bound PS microparticles was approximately twice that achieved with citrate-stabilized AuNPs or biotin-modified AuNPs. Furthermore, the detection sensitivity of 4-ATP was enhanced by introducing AuNP-bound PS microparticles into a microfluidic chip and delivering an aqueous 4-ATP solution under controlled flow conditions. Analysis of the flow rate dependence of SERS signal intensity revealed that the optimal detection sensitivity for 4-ATP was achieved at a flow rate of 0.66 μL·min⁻<sup>1</sup>, with a corresponding detection limit of 1.9 μM under these conditions. These results demonstrate that AuNP-bound PS microparticles introduced into the microfluidic chip serve as efficient SERS substrates, enabling highly sensitive and quantitative detection under flow conditions.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 11","pages":"1801 - 1809"},"PeriodicalIF":2.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and application of pretreatment for the analysis of typical per- and polyfluoroalkyl substances (PFAAs) in drinking water: a systematic evaluation of filter membranes and SPE Sorbents 饮用水中典型的全氟烷基和多氟烷基物质（PFAAs）分析的预处理优化与应用：过滤膜和SPE吸附剂的系统评价。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-09-09 DOI: 10.1007/s44211-025-00847-0

Duwang Li, Wanyuan He, Lingling Liu, Wanlu Ren, Yiqi Wan, Huiping Deng, Hongwei Yang

{"title":"Optimization and application of pretreatment for the analysis of typical per- and polyfluoroalkyl substances (PFAAs) in drinking water: a systematic evaluation of filter membranes and SPE Sorbents","authors":"Duwang Li, Wanyuan He, Lingling Liu, Wanlu Ren, Yiqi Wan, Huiping Deng, Hongwei Yang","doi":"10.1007/s44211-025-00847-0","DOIUrl":"10.1007/s44211-025-00847-0","url":null,"abstract":"<div><p>The accurate detection of trace perfluoroalkyl acids (PFAAs) in drinking water remains challenging due to nonspecific adsorption losses during pretreatment. This study systematically evaluated the adsorption behaviors of 11 PFAAs across five filtration membranes and four solid-phase extraction (SPE) sorbents to establish an optimized analytical protocol. Results demonstrated that glass fiber (GL) filters minimized PFAAs retention (94.85% recovery in aqueous matrices) through electrostatic repulsion, outperforming other membranes such as polypropylene(PP). Among SPE sorbents, Weak Anion Exchange (WAX) exhibited the highest recovery rates among the sorbents evaluated (100.04% ± 2.74% recovery) via a dual adsorption mechanism combining amine-mediated ion exchange and hydrophobic interactions, achieving 30% higher efficiency than Hydrophile-Lipophile Balance (HLB) sorbents. The optimized method achieved low detection limits (LOD: 0.016–0.324 ng/L), high accuracy (spiked recovery: 80.12–115.76%) and precision (RSD < 10%). Method application revealed significantly lower PFAAs contamination in reservoir-sourced finished water compared to river/lake-derived supplies in the mid-Yangtze River region. This work effectively mitigates critical pretreatment biases in PFAAs analysis and provides a reliable solution to address the urgent need for monitoring PFAAs contamination in drinking water systems.</p><h3>Graphic abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 12","pages":"1863 - 1873"},"PeriodicalIF":2.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of phenylphosphonic acid zinc salt in poly(lactic acid) using thermally assisted methylation pyrolysis–gas chromatography/mass spectrometry 热辅助甲基化热解-气相色谱/质谱法测定聚乳酸中苯基膦酸锌盐。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-09-08 DOI: 10.1007/s44211-025-00845-2

Kazuko Matsui, Makoto Niwa, Atsushi Watanabe, Chuichi Watanabe, Norio Teramae

{"title":"Determination of phenylphosphonic acid zinc salt in poly(lactic acid) using thermally assisted methylation pyrolysis–gas chromatography/mass spectrometry","authors":"Kazuko Matsui, Makoto Niwa, Atsushi Watanabe, Chuichi Watanabe, Norio Teramae","doi":"10.1007/s44211-025-00845-2","DOIUrl":"10.1007/s44211-025-00845-2","url":null,"abstract":"<div><p>Biomass-based polymers such as poly(lactic acid) (PLA) have attracted much attention, because they are renewable, biocompatible, and nontoxic to the environment and have been used in various fields such as biomedical, agricultural, and food packaging industries. However, one of the common drawbacks of PLA-based materials is their low glass transition temperature in the amorphous state, while adding phenylphosphonic acid zinc salt (PPA-Zn) as a nucleating agent was found to be a promising method to improve the physical property of PLA. On the other hand, degradation of PLA-based materials in the environment may cause the pollution from the metal of a nucleating agent in PLA and quantification of nucleating agents in polymers is of interest. Polar phosphonic acid, a ligand in PPA-Zn, is not favorable for the analysis by gas chromatography/mass spectrometry (GC/MS). Such compounds containing polar groups are known to be effectively analyzed by GC/MS in combination with thermally assisted methylation reaction. In this study, we report on determination of PPA-Zn in PLA by coupling thermally assisted methylation with pyrolysis–GC/MS.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 11","pages":"1817 - 1823"},"PeriodicalIF":2.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus 金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌快速检测方法研究进展。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-09-06 DOI: 10.1007/s44211-025-00843-4

Linlin Zhuang, Mengling Zhu, Xinya Chen, Xiaoming Liu, Tianyu Yuan, Chuang Zhou, Xue Lian, Yunming Xu, Yu Zhang, Qiuping Shen

{"title":"Advances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus","authors":"Linlin Zhuang, Mengling Zhu, Xinya Chen, Xiaoming Liu, Tianyu Yuan, Chuang Zhou, Xue Lian, Yunming Xu, Yu Zhang, Qiuping Shen","doi":"10.1007/s44211-025-00843-4","DOIUrl":"10.1007/s44211-025-00843-4","url":null,"abstract":"<div><p><i>Staphylococcus aureus</i> (<i>S. aureus</i>) and methicillin-resistant <i>S. aureus</i> (MRSA) are important pathogens that are closely associated with hospital-acquired and community-acquired infections. Rapid and accurate detection methods are important for early diagnosis and treatment of diseases. This paper reviews the latest research progress in the field of rapid detection of <i>S. aureus</i> and MRSA, including chromogenic media, immunological detection methods (e.g., enzyme-linked immunosorbent assays, lateral flow assays, colorimetric/fluorescent immunoassays, etc.), nucleic acid tests (including polymerase chain reaction-based methods, isothermal amplification techniques), biosensors, and novel emerging methods, etc. The advantages and disadvantages of these methods are systematically analyzed and discussed. Future research should focus on improving analytical sensitivity and specificity, shortening detection time, reducing detection cost, and promoting the integration of multiple platforms and intelligent analysis, especially for the development of technologies for point-of-care testing. These efforts are crucial to addressing the pressing need for rapid clinical diagnosis.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 11","pages":"1715 - 1747"},"PeriodicalIF":2.0,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In ovo sexing of chicken embryos using spectroscopic analysis 用光谱分析测定鸡胚胎的卵性别

IF 2 4区化学

Analytical Sciences Pub Date : 2025-09-03 DOI: 10.1007/s44211-025-00824-7

Mika Ishigaki

引用次数: 0

Investigation of experimental factors in in-capillary continuous enzyme assay to obtain plateau response by electrophoretically mediated microanalysis 毛细管内连续酶分析中电泳介导微量分析获得平台响应的实验因素研究。

IF 2 4区化学

Analytical Sciences Pub Date : 2025-08-28 DOI: 10.1007/s44211-025-00844-3

Toshio Takayanagi, Risa Okazaki, Karen Gotoda, Masanori Mine, Hitoshi Mizuguchi

{"title":"Investigation of experimental factors in in-capillary continuous enzyme assay to obtain plateau response by electrophoretically mediated microanalysis","authors":"Toshio Takayanagi, Risa Okazaki, Karen Gotoda, Masanori Mine, Hitoshi Mizuguchi","doi":"10.1007/s44211-025-00844-3","DOIUrl":"10.1007/s44211-025-00844-3","url":null,"abstract":"<div><p>In-capillary enzymatic reaction was investigated in electrophoretically mediated microanalysis to achieve plateau response of the reaction product. Hydrolysis of 4-nitrophenyl phosphate (NPP) with alkaline phosphatase (ALP) was used as a model enzymatic reaction. The reaction product of 4-nitrophenol (NP) was photometrically detected as a plateau response based on the continuous enzymatic reaction in the separation capillary and the ongoing electrophoretic resolution of the product from the reaction zone. Two types of injection were compared between an enzyme injection and a substrate injection, whereas the counterpart reagent was contained in the separation buffer. In both approaches, the plateau response of NP developed linearly with increasing sample injection period, as well as the enzyme concentration. At low separation voltages, NP accumulated at the reaction zone, and the plateau height was inversely proportional to the applied voltage. Comparing enzyme and substrate injection methods, a stable plateau response was more easily achieved with the enzyme injection. It is because the concentration of the substrate in the injected plug decreases during the electrophoresis, leading to a reduced reaction rate. In contract, enzyme injection maintains consistent reaction conditions. The Michaelis–Menten constant was determined with the plateau response by both injection modes, and the values were comparable with each other.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 11","pages":"1791 - 1799"},"PeriodicalIF":2.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0