Analytical Sciences最新文献

{"title":"Determination of hydrogen peroxide in commercial glycerin using electron spin resonance and chemiluminescence assay","authors":"Gotaro Shiota,&nbsp;Noriko Tajima,&nbsp;Hidetoshi Arakawa,&nbsp;Masaru Kato","doi":"10.1007/s44211-025-00861-2","DOIUrl":"10.1007/s44211-025-00861-2","url":null,"abstract":"<p>Glycerin is widely used in pharmaceuticals, cosmetics, and foods. In recent years, it has been mainly derived from plant-based materials, particularly palm oil. The most abundant polyphenol in palm oil is 3-<i>O</i>-caffeoylshikimic acid (CSA). Polyphenols reportedly react with oxygen to generate hydrogen peroxide (H₂O₂). In this study, we investigated the generation of H₂O₂ from CSA and palm-derived glycerin using the bis(2,4,6-trichlorophenyl)oxalate (TCPO) chemiluminescence method and electron spin resonance spectroscopy. H₂O₂ concentrations in the range of 10⁻⁴ to 10⁻⁶ mol/L were detected in commercial glycerin samples. In addition, the H₂O₂ concentrations in a commercial glycerin varied widely between batches, even among samples of glycerin produced by the same company. However, the concentrations were approximately 1/1000 of that permitted in disinfectants used in pharmaceuticals, indicating extremely low biological toxicity. Furthermore, the TCPO chemiluminescence method proved to be highly sensitive and precise, even in viscous glycerin samples, showing potential for application in quality control of glycerin formulations and related industrial products.</p>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 1","pages":"65 - 70"},"PeriodicalIF":2.0,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145803022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermochemolysis-gas chromatographic detection of oxidized fatty acids in a single Drosophila melanogaster individual","authors":"Yasuyuki Ishida,&nbsp;Misaki Haba,&nbsp;Noa Komoda,&nbsp;Nobuo Kido,&nbsp;Atsushi Watanabe","doi":"10.1007/s44211-025-00860-3","DOIUrl":"10.1007/s44211-025-00860-3","url":null,"abstract":"<div><p>Thermochemolysis-gas chromatography–mass spectrometry (Thermochemolysis-GC–MS) was applied to detect fatty acid oxidation products in a single <i>Drosophila melanogaster</i> individual (dry weight: 100–400 µg). Linoleic acid oxidized under UV irradiation was first analyzed to identify characteristic oxidation products, including azelaic acid. Using tetramethylammonium hydroxide (TMAH) as an organic alkali reagent, azelaic acid was successfully detected as its dimethyl ester in <i>D. melanogaster</i> individuals reared on linoleic acid–supplemented diets. The use of <i>m/z</i> 185 as a diagnostic ion in extracted ion chromatograms allowed for sensitive determination of azelaic acid in control and linoleic acid–treated <i>D. melanogaster</i> individuals. The treated groups exhibited notable increases in azelaic acid content, which demonstrates the method’s ability to evaluate statistically significant differences based on individual-level data.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 1","pages":"59 - 64"},"PeriodicalIF":2.0,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing peroxidase-like activity of Cu/Ag nanoclusters nanozyme by G/C-rich sequence for colorimetric determination of ochratoxin A","authors":"Shaojie Xia,&nbsp;Zhenyuan Yang,&nbsp;Xiaohui Sun,&nbsp;Junjie Huang,&nbsp;Kuan Peng,&nbsp;Yonghong Wang","doi":"10.1007/s44211-025-00857-y","DOIUrl":"10.1007/s44211-025-00857-y","url":null,"abstract":"<div><p>Ochratoxin A (OTA), a highly toxic mycotoxin, extensively contaminates a wide range of food commodities. Given increasing demands for sensitive and user-friendly detection platforms, we developed a colorimetric OTA biosensor leveraging copper-silver nanoclusters (Cu/Ag NCs), whose peroxidase-like activity is augmented via G/C-rich DNA hybridization. The system employs two tailored DNA strands: a template strand featuring a cytosine-rich domain for NC synthesis flanked by terminal recognition segments and an enhancer strand containing a guanine-rich signal amplification domain and a complementary linker. Upon OTA binding with the aptamer, the apt-template complex dissociates, facilitating template-enhancer hybridization to form G/C-rich double-stranded DNA. This structural transition substantially enhances the peroxidase-like activity of DNA-Cu/Ag NCs, accelerating the oxidation of 3,3′,5,5′-tetramethylbenzidine by H₂O₂ while simultaneously amplifying the colorimetric signal. Quantitative OTA detection is accomplished by monitoring this dynamic color response. The biosensor demonstrates a semi-log linear detection range from 5.0 nM to 1.0 μM with a detection limit of 5.0 nM. Furthermore, the specific test has high selectivity for other common mycotoxins, and its applicability has been validated through real sample analysis, suggesting that the DNA-Cu/Ag NCs biosensor used for OTA detection holds promising practical applications in food samples.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 2","pages":"79 - 88"},"PeriodicalIF":2.0,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s44211-025-00857-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aggregation-induced emission luminogens for volatile organic compound detection","authors":"Muneeba Amin,&nbsp;Iqra Gulzar,&nbsp;Qurashi Najmu Saqib,&nbsp;Muzaffer Ahmad,&nbsp;Adil Majeed Rather,&nbsp;Nusrat Shafi,&nbsp;Waseem A. Wani,&nbsp;Jahangir Ahmad Rather,&nbsp;Waheed Ahmad Khanday,&nbsp;Abdul Haleem Wani,&nbsp;Akhtar Hussain Malik,&nbsp;Faiza Habib","doi":"10.1007/s44211-025-00856-z","DOIUrl":"10.1007/s44211-025-00856-z","url":null,"abstract":"<div><p>Volatile organic compounds (VOCs) are crucial components in many daily-use products, yet they pose a significant threat to public health and the environment, even in minute amounts, due to their high toxicity. Therefore, it is crucial to develop a reliable method that could track these hazardous vapours with high sensitivity and selectivity. Among various techniques, fluorescence-based detection strategies have demonstrated great efficiency in both qualitative and quantitative monitoring of VOCs in real-time. These methods are attractive due to their low cost and portability, making them suitable for on-site applications. This review explores the latest advancements in aggregation-induced emission (AIE)-based fluorescent probes tailored for VOC detection across diverse platforms, highlighting their selectivity, sensitivity, and real-world applications. The use of AIE events has been justified for the development of selective and sensitive sensors for VOC detection. Furthermore, the present work investigates the various sensing mechanisms involved in predicting the AIE nature of the fluorophores. Additionally, we discuss existing challenges and propose future directions for designing next-generation AIE-based VOC sensors with enhanced specificity, stability, and practical usability. Coupled with the latest advancements, AIE-based fluorescence sensing of VOCs could contribute to the structural design of future smart VOC sensors that are highly efficient and effective.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 1","pages":"3 - 20"},"PeriodicalIF":2.0,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145712941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent development of surface treatment of polytetrafluoroethylene","authors":"Ryoichi Ishimatsu","doi":"10.1007/s44211-025-00853-2","DOIUrl":"10.1007/s44211-025-00853-2","url":null,"abstract":"","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 12","pages":"1825 - 1827"},"PeriodicalIF":2.0,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145584859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of surface-functionalized power-free microchip for breast cancer cell-derived extracellular vesicle detection","authors":"Ryo Ishihara,&nbsp;Ryo Matsui,&nbsp;Tadaaki Nakajima,&nbsp;Hinako Yokohari,&nbsp;Ren Ogata,&nbsp;Kotomi Katori,&nbsp;Kazuo Hosokawa,&nbsp;Mizuo Maeda,&nbsp;Akihiko Kikuchi","doi":"10.1007/s44211-025-00854-1","DOIUrl":"10.1007/s44211-025-00854-1","url":null,"abstract":"<div><p>Extracellular vesicles (EVs) have emerged as promising biomarkers for liquid biopsies because of their responsiveness to various physiological conditions. However, their dynamic physical properties pose significant analytical challenges, which necessitates the development of novel detection methods. In this study, a portable surface-functionalized power-free microchip (SF-PF microchip) was developed that enables the detection of EVs without external pumps. In particular, the SF-PF microchip specifically detected EVs derived from metastatic breast cancer cells with a limit of detection of 5.0 × 10¹⁰ particles/mL. Moreover, the SF-PF microchip required only 2.0 µL of sample volume and completed the detection within approximately 20 min, offering a rapid and convenient approach for EV analysis. Furthermore, the SF-PF microchip successfully detected EVs in serum samples, which highlights its potential for clinical application in liquid biopsies.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"42 1","pages":"37 - 47"},"PeriodicalIF":2.0,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145562474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sample pretreatment approaches for improving versatility and convenience in gas chromatographic analysis","authors":"Yasuyuki Ishida","doi":"10.1007/s44211-025-00848-z","DOIUrl":"10.1007/s44211-025-00848-z","url":null,"abstract":"","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 11","pages":"1695 - 1696"},"PeriodicalIF":2.0,"publicationDate":"2025-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145476361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomineralized gold nanoparticle-based colorimetric sensing platform for ascorbic acid detection","authors":"Zhuqing Wang,&nbsp;Junyuan Zhang,&nbsp;Yuetong Ma,&nbsp;Mengran Zhang,&nbsp;Huixue Zhang,&nbsp;Bing Yang,&nbsp;Qiaorong Tang","doi":"10.1007/s44211-025-00852-3","DOIUrl":"10.1007/s44211-025-00852-3","url":null,"abstract":"<div><p>Ascorbic acid (AA), a critical regulator of redox homeostasis, plays a vital role in disease prevention. Thus, the development of a rapid and precise detection method is urgently needed. In this work, a colorimetric sensing platform based on bioinspired mineralization-synthesized gold nanoparticles (AuNPs) was proposed for the visual and rapid detection of AA. The as-prepared AuNPs exhibited uniform morphology and excellent oxidase-like activity, efficiently catalyzing 3,3',5,5'-tetramethylbenzidine (TMB) oxidation to produce a characteristic 650 nm absorption peak. However, in the presence of AA, TMB oxidation was significantly inhibited due to the reducing property of AA. The color change was visually detectable within 5 min. The absorbance at 650 nm linearly decreased with the AA concentration in the range of 1–60 μM (R² = 0.999). The detection limit was estimated to 0.27 μM. Besides, the selectivity was excellent against common interfering species. The detection of AA in complex fruit samples was realized, and the recovery rate was 98.5–106.1%. Owing to the simplicity, cost-effectiveness, high sensitivity, and rapid response, this colorimetric sensing strategy provides new insights into the on-site monitoring of antioxidants in biological samples.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 12","pages":"1893 - 1900"},"PeriodicalIF":2.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solid-phase extraction of ethyleneamines in river water prior to their determination by high-performance liquid chromatography with ultraviolet detection","authors":"Azusa Yudo,&nbsp;Tomoko Kemmei,&nbsp;Shuji Kodama,&nbsp;Yoshinori Inoue,&nbsp;Shigehiro Kagaya","doi":"10.1007/s44211-025-00850-5","DOIUrl":"10.1007/s44211-025-00850-5","url":null,"abstract":"<div><p>A method based on solid-phase extraction (SPE) has been developed for the preconcentration of three kinds of ethyleneamines (EAs) [ethylenediamine (EDA), diethylenetriamine (DETA), and triethylenetetramine (TETA)] in river water for their high-performance liquid chromatographic (HPLC) determination by using on-line complexation with Cu(II) ion and ultraviolet (UV) detection. EAs, which are protonated in acidic and neutral conditions, could be concentrated by using cation-exchange SPE. However, inorganic cations and humic substances, often present in river water, interfered with this preconcentration. Inorganic cations, such as Ca and Mg ions, compete with EAs in cation-exchange processes. This interference could be reduced by masking them with ethylenediaminetetraacetic acid. Humic substances, such as humic acid and fluvic acid, seem to be due to the electrostatic interaction of EAs with them in the sample solution. This interference was reduced by passing the sample solution through an anion-exchange cartridge before the cation-exchange SPE. The interacted EAs remained in the anion-exchange cartridge; they could be eluted by washing out the anion-exchange cartridge with diluted HCl. In this washing process, although fulvic acid was eluted at the same time, it could be removed by passing the eluate through a reversed-phase cartridge connected in a series. After removing these interferences, EAs could be extraced with the cation-exchange SPE and quantitatively eluted by passing Cu(II) solution through the cartridge. The proposed method of including the HPLC–UV combined with the SPE was available for the determination of EAs in river water at concentrations as low as 0.015 µM for EDA and DETA and 0.05 µM for TETA. The high recoveries (83%–103%) and repeatabilities (RSD 1.2%–4.3%) were obtained for EAs from two river water samples spiked with two different concentrations of EAs. The proposed method was applied to analyses of river water samples taken from four rivers in Toyama Prefecture, Japan.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 12","pages":"1883 - 1892"},"PeriodicalIF":2.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virus inactivation effect of hypochlorite solution containing nitrate ions","authors":"Erina Satake,&nbsp;Maho Ishiyama,&nbsp;Masaki Sekiguchi,&nbsp;Atsushi Sasaki,&nbsp;Shigekazu Yano,&nbsp;Masatoshi Endo","doi":"10.1007/s44211-025-00831-8","DOIUrl":"10.1007/s44211-025-00831-8","url":null,"abstract":"<div><p>Hypochlorous acid (HClO) is widely used as a disinfectant due to its strong oxidizing power, but its efficacy is highly dependent on pH and decreases under neutral to alkaline conditions. In addition, its high concentration poses safety and environmental concerns. In this study, we investigated the enhancement of virucidal activity at lower HClO concentrations through the formation of monochloramine (NH₂Cl) by supplementing with ammonium-form nitrogen (NH₄⁺–N) or nitrate-form nitrogen (NO₃⁻–N) under different pH conditions. Using bacteriophage φ6 as an enveloped virus model, plaque assays revealed that virus inactivation was most effective at pH 8 when 15–20 ppm of NH₄⁺–N was added to 30 ppm HClO solution, with over 90% reduction in infectivity. Under acidic conditions (pH 6), the formation of NH₂Cl was limited due to the rapid consumption of free chlorine and insufficient generation of NH₃, resulting in reduced virucidal efficacy; however, increasing the amount of NH₄⁺–N led to improved inactivation effects. In contrast, at pH 8, NH₂Cl formation was enhanced, contributing to improved disinfection efficiency. Similar effects were observed when NO₃⁻–N was used, likely due to its reduction to NH₄⁺ in the presence of organic matter. These results demonstrate that optimizing pH and nitrogen sources can improve the efficacy of low-concentration HClO solutions by generating NH₂Cl in situ, offering a safer and more sustainable approach to viral disinfection.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7802,"journal":{"name":"Analytical Sciences","volume":"41 12","pages":"1853 - 1862"},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}