Microfluidic devices integrated with track-etched porous membranes for cell-based bioanalysis.

Abstract

Microfluidic devices integrated with track-etched porous membranes are useful for cell-based bioanalysis. In this mini review, our latest achievements based on membrane-integrated microfluidic devices are reviewed. The membrane-integrated microfluidic devices in parallel configuration have been employed to develop a microfluidic model of microcirculation and for the development of nanomedicines. Co-culture of endothelial cells with tumor cells and parallel permeation assays through keratinocyte cell layer have also been demonstrated. Membrane-integrated microfluidic devices in perpendicular configuration have been employed to simulate extravasation of tumor cells and to evaluate the efficacy of anti-inflammatory drug on the function of keratinocyte cell layer formed on the membrane. These microfluidic devices have unique features and a lot of advantages, so we hope that they are utilized as a powerful tool for cell-based bioanalysis.