Food & Function最新文献

{"title":"Investigating the modulatory effects of Pu-erh tea on the gut microbiota in ameliorating hyperuricemia induced by circadian rhythm disruption†","authors":"Sibo Zhao, Hongli Cao, Fanwei Sun, Mianhong Xu, Xinghua Wang, Jielin Jiang, Liyong Luo and Liang Zeng","doi":"10.1039/D4FO05659K","DOIUrl":"10.1039/D4FO05659K","url":null,"abstract":"<p >Circadian rhythm disruption (CRD) can induce a variety of metabolic disorders. Our previous laboratory studies have shown that Pu-erh tea could alleviate CRD-induced syndromes, including obesity, intestinal dysfunction, and tryptophan metabolism disorders. However, its potential protective mechanism against CRD-induced hyperuricaemia remains unclear. In this work, we found that polyphenols of Pu-erh tea were significantly released in the stage of intestinal digestion, which might promote their interaction with gut microbes. Through animal experiments, C57BL6/J mice were given water or different doses of Pu-erh tea for 60 days, followed by a 90-day CRD, the lifestyle of modern individuals who frequently stay up late. Our results indicated that CRD mice exhibited high serum uric acid levels and gut microbiota disorders. Pu-erh tea intake significantly reshaped the gut microbiome, especially increasing the abundance of <em>Bifidobacterium</em>, <em>Akkermansia</em> and <em>Faecalibaculum</em>, and increased the production of short-chain fatty acids (SCFAs), especially acetic acid, which restored the function of the intestinal barrier. This improvement further regulated oxidative stress pathways (NRF2/HO-1), reduced systemic inflammatory response (IL-6, IL-1β, and TNF-α), restored hepatic function (SOD, MOD, CAT, and GSH) and modulated the activity of enzymes related to UA metabolism in the liver (XOD and ADA). Finally, Pu-erh tea intake promoted the excretion of UA and reduced the levels of UA and xanthine in the serum. Moreover, the results of antibiotic experiments showed that the UA improvement effect of Pu-erh tea depended on the existence of the gut microbiota. Collectively, Pu-erh tea intake has the potential to prevent CRD-induced hyperuricaemia by reshaping the gut microbiota.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 7","pages":" 2669-2686"},"PeriodicalIF":5.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative effects of different sugar substitutes: Mogroside V, stevioside, sucralose, and erythritol on intestinal health in a type 2 diabetes mellitus mouse†","authors":"Huaxue Huang, Sha Liu, Zhi Peng, Bin Wang, Shuang Zhan, Sirui Huang, Wei Li, Dai Liu, Xiulian Yang, Yizhun Zhu and Wenjun Xiao","doi":"10.1039/D4FO04446K","DOIUrl":"10.1039/D4FO04446K","url":null,"abstract":"<p >Intestinal health disorders significantly contribute to the development of type 2 diabetes mellitus (T2DM). Sugar substitutes such as mogroside V (MOG), stevioside (ST), sucralose (TGS), and erythritol (ERT), are increasingly used in T2DM management as alternatives to sucrose (SUC). However, their effects on intestinal health in T2DM have not been fully compared. In the present study, we established a T2DM mouse model using a high-fat diet and streptozotocin injection. These mice were treated with equal doses of SUC, MOG, ST, TGS, or ERT for 4 weeks to evaluate the effects of these sugar substitutes on intestinal health in T2DM. T2DM mice exhibited increased intestinal permeability, reduced goblet cell numbers, elevated pro-inflammatory cytokine levels, and alterations in both gut microbiota and metabolite composition. After 4 weeks of treatment, MOG showed the most significant benefits. MOG activates the PI3K/AKT pathway, enhancing the expression of tight junction proteins, which improves intestinal barrier function and reduces permeability. This is accompanied by NF-κB inhibition, leading to reduced pro-inflammatory cytokine production and increased mucus secretion. These changes help maintain healthy gut microbiota and metabolites, preventing pathogenic bacteria from entering the bloodstream. ST downregulates NF-κB to alleviate intestinal inflammation and improves gut microbiota and metabolic homeostasis in T2DM. ERT has less beneficial effects. TGS and SUC reduce intestinal inflammation and have a better effect on the duodenum. However, TGS has a negative effect on the colon microbiota and metabolites, whereas SUC has a negative effect on the colon microbiota alone. MOG improved intestinal health in T2DM by modulating the PI3K/AKT and NF-κB pathways, whereas ST primarily modulated NF-κB to alleviate intestinal inflammation. Both treatments were effective, with MOG showing the best performance. Therefore, MOG can be considered a viable alternative to SUC for T2DM management.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 5","pages":" 2108-2123"},"PeriodicalIF":5.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of long-term moderate red wine consumption in CD1 female mice","authors":"Emilia Garcia-Moruno, Ana-Belén Blázquez, Nereida Jiménez de Oya, Ana Esteban, Silvia Motta, Antonella Costantini, Pedro J. Sánchez-Cordón, Eva Pericuesta, Miguel A. Martín-Acebes, Alfonso Gutiérrez-Adán and Juan-Carlos Saiz","doi":"10.1039/D5FO00435G","DOIUrl":"10.1039/D5FO00435G","url":null,"abstract":"<p >In the early 80s, the so-called “French paradox” was proposed, that is, a correlation between wine consumption, a diet rich in fats, and low mortality from coronary disease. Conversely, it is well established that excessive alcohol consumption increases the risk of cirrhosis and cancer, but few studies have investigated the effects of moderate alcohol consumption. However, all these conclusions were derived from epidemiological population studies that may be subject to distortions due to multiple factors. Here, the effect of moderate consumption of red wine on health throughout life was examined in a murine model. Different variables were evaluated in groups of female animals that were fed a standard or a fat diet throughout their adult life and given water, or wine or alcohol diluted in water in proportions similar to what is considered moderate consumption in humans. Our results showed few differences in most of the analyzed variables (body weight, liver profile and survival rate) between the different female mouse groups. The most remarkable findings were observed in the fat-diet groups that showed more frequent and severe liver lesions and a lower average ovarian weight. Moreover, moderate and prolonged ethanol consumption significantly affected telomere length only when the diet was high in fat, whereas wine consumption showed no difference compared to water, pointing to a possible predominant role of the compounds, particularly polyphenols present in wine. On the other hand, wine-drinking mice fed a fat diet had more oocytes than those in the ethanol-drinking group. Overall, our data suggest that long-term moderate red wine consumption does not substantially influence the health of female mice.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 7","pages":" 2656-2668"},"PeriodicalIF":5.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fo/d5fo00435g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indian lychee honey ameliorates hepatic glucose uptake by regulating the ChREBP/Glut4 axis under insulin-resistant conditions†","authors":"Chandrachur Ghosh, Tathagata Kundu, Tiyasa Pathak, Saakshi Saini, Neeladrisingha Das, Surendra Saini, Debabrata Sircar, Prabhat Kumar and Partha Roy","doi":"10.1039/D4FO03900A","DOIUrl":"10.1039/D4FO03900A","url":null,"abstract":"<p >Many traditional treatments include honey owing to its magnificent health beneficiary effects. Recent studies have demonstrated the potent anti-diabetic activity of honey. However, its actual mechanism of action remains elusive. Moreover, being rich in sugar (75%–80%), its role in maintaining glucose homeostasis remains questionable. Although the polyphenol content of honey aids its hypoglycaemic activity, the small quantity of bioactive compounds in honey (0.5%–1.0%) may not be solely responsible for this. In the current study, an attempt was made to understand the role of Indian lychee honey (LyH) in regulating blood glucose levels under diabetic conditions. This study investigated whether LyH, although rich in sugars, can be used as an alternative to regulate glucose and lipid homeostasis under insulin-resistant conditions by regulating the ChREBP/Glut4 signalling pathway. This study was first performed <em>in vitro</em> in palmitic acid-induced insulin-resistant HepG2 cells. Various assays, such as FACS, GCMS, qRT-PCR, immunoblot and ChIP-qPCR, were performed to establish the anti-hyperglycaemic role of LyH <em>in vitro</em>. The <em>in vitro</em> results were subsequently confirmed <em>in vivo</em> using a high-fat diet-induced diabetic C57BL/6 mice model. The <em>in vivo</em> study was supported by several experiments, such as examining blood parameters, histopathology, double-immunohistochemistry and ELISA. Finally, the finding was validated by comparing it with a couple of GEO datasets from the NCBI database. This study found that LyH is an excellent choice for regulating blood sugar levels under diabetic conditions without significant harmful side effects. Moreover, LyH showed excellent hepatic glucose uptake activity in an insulin-independent manner. This activity is mainly governed by sugars as its main ingredient. LyH treatment also regulates hepatic lipid homeostasis by maintaining a balance between saturated and unsaturated fatty acids in insulin-resistant HepG2 cells. Further, sugar, when supplemented individually, caused severe inflammation, which was validated through histopathology, ELISA and IHC. Collectively, the findings of this study indicate that Indian LyH provides a better food matrix (the right proportion of sugars and different bioactive compounds), which significantly improves hyperglycemia and inflammation under diabetic conditions by regulating the hepatic ChREBP/Glut4 axis.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 5","pages":" 2031-2056"},"PeriodicalIF":5.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From in silico screening to in vivo validation in zebrafish – a framework for reeling in the right psychobiotics†","authors":"Benjamin Valderrama, Isabelle Daly, Eoin Gunnigle, Kenneth J. O'Riordan, Maciej Chichlowski, Sagarika Banerjee, Alicja A. Skowronski, Neeraj Pandey, John F. Cryan, Gerard Clarke and Jatin Nagpal","doi":"10.1039/D4FO03932G","DOIUrl":"10.1039/D4FO03932G","url":null,"abstract":"<p >The potential of gut bacteria to interact with the nervous system is now well known. Therefore, the characterization of bacterial strains that can modulate signalling pathways of the nervous system is a topic of growing interest, as it represents a potential alternative therapeutic target to treat central nervous system disorders. However, a streamlined screening framework is required to guide the rational identification and selection of such bacteria, known as psychobiotics. In this work, we introduce a framework that integrates <em>in silico</em>, <em>in vitro</em> and <em>in vivo</em> approaches to identify psychobiotic candidates capable of both metabolising prebiotics of interest and producing neuroactive molecules. To prove the effectiveness of the approach, we characterized a bacterial strain, <em>Lactiplantibacillus plantarum</em> APC2688, for its capacity to modulate the GABAergic system and alter the stress-related behaviour of zebrafish larvae. In brief, <em>in silico</em> analyses of the genomic content of APC2688 identified it as capable of degrading different prebiotics and producing neuroactive compounds known to modulate the stress response in animal models. Then, <em>in vitro</em> results confirmed the ability of this strain to produce GABA, tryptophan and acetate, while growing with the candidate prebiotics of interest, fructooligosaccharides (FOS), galactooligosaccharides (GOS) and inositol. <em>In vivo</em> experiments demonstrated that the administration of bacterial supernatants induced changes in the expression of <em>gad1</em> and <em>gabra1</em> in zebrafish larvae, two essential genes in the GABAergic signalling pathway, and altered the anxiety-like behaviour of the larvae. These results highlight the efficiency of our framework in integrating orthogonal approaches to discover and characterise bacteria capable of modulating the microbiome–gut–brain axis.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 5","pages":" 2018-2030"},"PeriodicalIF":5.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fo/d4fo03932g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationships of sarcopenia symptoms and dietary patterns with lung cancer risk: a prospective cohort study†","authors":"Huijun Zhou, Fubin Liu, Jingyi Xu, Xixuan Wang, Yu Peng, Peng Wang, Changyu Si, Jianxiao Gong, Jiale Gu, Ailing Qin and Fangfang Song","doi":"10.1039/D4FO03332A","DOIUrl":"10.1039/D4FO03332A","url":null,"abstract":"<p > <em>Background</em>: Few studies focused on the effects of sarcopenia on lung cancer in the general population and optimizing nutritional intake may be a feasible way to manage sarcopenia. We sought to systematically investigate the associations of sarcopenia symptoms with lung cancer incidence and mortality in the general population, and whether dietary patterns could modify these risks. <em>Methods</em>: A total of 361 763 participants (mean age: 56.2 years; and men: 46.7%) were included in this prospective UK Biobank study. Sarcopenia symptoms (low handgrip strength, low muscle mass and slow walking pace) were determined according to European Working Group of Sarcopenia in Older People 2 (EWGSOP2) criteria. Individuals without any of the three sarcopenia symptoms were classified as the normal control group. Based on the baseline data from food frequency questionnaires, primary dietary patterns were identified through principal component analysis. Cox proportional hazards models were used to examine associations between sarcopenia symptoms as well as dietary patterns and lung cancer. Then we explored the joint effects of sarcopenia symptoms and dietary patterns on lung cancer risk. <em>Results</em>: A total of 3532 incident lung cancer cases and 2073 deaths were documented during a median follow-up of 12 years. All the sarcopenia symptoms were associated with a higher risk of lung cancer incidence than the normal control group, especially in people aged &lt;60 (<em>P</em><small>_interaction</small> &lt; 0.05). Particularly, a stronger association was observed for slow walking pace with incidence (hazard ratio [HR]: 1.49, 95% confidence interval [CI]: 1.34–1.65) and mortality (HR: 1.54, 95% CI: 1.35–1.75) of lung cancer. Higher adherence to the wholegrain pattern was associated with a greater reduction in the risk of lung cancer incidence (HR<small>_{Q4<em>vs.</em>Q1}</small>: 0.71, 95% CI: 0.65–0.79) and mortality (HR<small>_{Q4<em>vs.</em>Q1}</small>: 0.68, 95% CI: 0.60–0.77). The joint analysis demonstrated that the risk of lung cancer-related outcomes associated with low handgrip strength gradually reduced as the quartile of wholegrain pattern scores increased (<em>P</em><small>_trend</small> &lt; 0.05). <em>Conclusions</em>: Our study indicated that individuals with sarcopenia symptoms suffered a higher risk of lung cancer incidence and mortality even in younger age. A diet abundant in whole grains may help to improve sarcopenia symptoms and reduce adverse lung cancer-related outcomes associated with low handgrip strength.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 6","pages":" 2432-2443"},"PeriodicalIF":5.1,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fo/d4fo03332a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Protective effects of potential probiotic Lactobacillus rhamnosus (MTCC-5897) fermented whey on reinforcement of intestinal epithelial barrier function in a colitis-induced murine model","authors":"Harpreet Kaur, Taruna Gupta, Suman Kapila and Rajeev Kapila","doi":"10.1039/D5FO90014J","DOIUrl":"10.1039/D5FO90014J","url":null,"abstract":"<p >Correction for ‘Protective effects of potential probiotic <em>Lactobacillus rhamnosus</em> (MTCC-5897) fermented whey on reinforcement of intestinal epithelial barrier function in a colitis-induced murine model’ by Harpreet Kaur <em>et al.</em>, <em>Food Funct.</em>, 2021, <strong>12</strong>, 6102–6116, https://doi.org/10.1039/D0FO02641G.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 5","pages":" 2156-2157"},"PeriodicalIF":5.1,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fo/d5fo90014j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tamarindus indica sub-products as potential tools for simultaneous management of diabetes and obesity†","authors":"Gustavo Henrique Souza, Beatriz Paes Silva, Gabriel Arcanjo Viana Neto, Tiane C. Finimundy, Thalita Faleiro Demito Santos, Paulo Sergio Alves Bueno, Felipe de Oliveira Souza, Eduardo J. Pilau, Lillian Barros, Jurandir F. Comar, Livia Bracht, Rosane M. Peralta, Adelar Bracht and Anacharis B. Sá-Nakanishi","doi":"10.1039/D4FO04536J","DOIUrl":"10.1039/D4FO04536J","url":null,"abstract":"<p >Aiming at valorizing industrial wastes of tamarind, the present work evaluated the actions of seed, leaf and peel extracts on starch and fat absorption through starch and triglyceride tolerance tests in mice. The actions of all extracts on the α-amylase and lipase were also characterized using classical kinetic assays. All extracts inhibited starch digestion <em>in vivo</em>, but the seed extract was the most effective one with an ID<small>₅₀</small> of 151.4 mg kg<small>⁻¹</small>. The mechanism behind this inhibition probably involves the pancreatic α-amylase, which was strongly inhibited by the seed extract under <em>in vitro</em> conditions (IC<small>₅₀</small> = 13.3 μg mL<small>⁻¹</small>) and much less strongly by the leaf and peel extracts (IC<small>₅₀</small> values in the vicinity of 400 μg mL<small>⁻¹</small>). The pancreatic lipase, conversely, was inhibited solely by the seed extract, with an IC<small>₅₀</small> of 31.5 μg mL<small>⁻¹</small>. As a consequence of this property, the seed extract also inhibited triglyceride absorption, as indicated by olive oil tolerance tests, which revealed an ID<small>₅₀</small> of 214.9 mg kg<small>⁻¹</small>. The seed extract also possessed the strongest antioxidant capacity and the highest content in phenolic groups. Chemical analyses revealed that all extracts present a great variety of phenolic compounds and that the seed extract possesses at least 5 unique compounds (ursodiol, masoprocol, lenticin, chenodeoxycolic acid and 13-keto-9<em>Z</em>,11<em>E</em>-octadecadienoic acid), which, according to docking studies, could be involved in the inhibition of both α-amylase and lipase. The overall conclusion is that the tamarind seed extract displays great potential for being used in the management of obesity and diabetes.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 5","pages":" 1969-1991"},"PeriodicalIF":5.1,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the suitability of sunflower press-cake proteins in formulated sports beverages†","authors":"Girotto Francesca, Ceccanti Costanza, Narra Federica and Piazza Laura","doi":"10.1039/D4FO04530K","DOIUrl":"10.1039/D4FO04530K","url":null,"abstract":"<p >In a context where whey proteins currently dominate the protein sports beverage sector and pea proteins are usually commercialized as protein sources, this study proposes using sunflower press-cake, which contains approximately 44% protein, as an alternative and sustainable protein source. After the extraction from the press-cake, sunflower proteins were dispersed in an aqueous medium with varying xanthan gum concentrations (0.2%, 0.4% and 0.6% w/v) to simulate protein-rich sports beverages. Their performance was compared to that of whey and pea proteins, each at a 10% concentration. To enhance protein dispersibility and align with the trends of alkaline beverages, a K–P buffer at pH 9 was used as the dispersion medium, and 0.2% caffeine was included for cognitive benefits. Pasteurized dispersions were tested for rheological behavior and physical stability at 4 °C. Sunflower proteins, with a total polyphenolic content (TPC) of 35.2 mg<small>_GAE</small> g<small>_DB</small><small>⁻¹</small>, outperformed whey (0.8 mg<small>_GAE</small> g<small>_DB</small><small>⁻¹</small>) and pea (2.8 mg<small>_GAE</small> g<small>_DB</small><small>⁻¹</small>) proteins. Sunflower dispersions exhibited a significantly lower volatile profile than those enriched with pea or whey proteins, reducing the need for odor-masking agents. Additionally, sunflower dispersions had a lower flow index than whey or pea dispersions, indicating easier processing. Despite the promising flow behaviour and optimal physical stability (stability index &lt; 1.0), <em>in vitro</em> bioaccessibility analysis revealed a similar percentage of protein bioaccessibility between pea and whey dispersions, while sunflower ones had the lowest percentage. TPC bioaccessibility followed a similar trend. These findings highlight the feasibility of incorporating sunflower proteins into sports beverages, broadening options for formulators and promoting sustainability by repurposing agricultural by-products and adopting plant-based proteins.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 5","pages":" 1992-2003"},"PeriodicalIF":5.1,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fo/d4fo04530k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of consumption of anthocyanin-rich products on NMR lipoprotein subclasses and biomarkers in hypercholesterolemic subjects: a randomized controlled trial (the AppleCOR study)†","authors":"A. Pedret, E. Llauradó, L. Calderón-Pérez, J. Companys, L. Pla-Pagà, P. Salamanca, B. A. Sandoval-Ramírez, M. Besora-Moreno, Ú. Catalán, S. Fernández-Castillejo, I. Ludwig, A. Macià, L. Rubió-Piqué, M. Sampson, A. T. Remaley, R. M. Valls, M. J. Motilva and R. Solà","doi":"10.1039/D4FO02949F","DOIUrl":"10.1039/D4FO02949F","url":null,"abstract":"<p >Our aim was to assess the effect of intake of anthocyanin biofortified red-fleshed apples (RFA) <em>versus</em> that of common white apples (WFA) without anthocyanins on the NMR lipoprotein subfraction profile and other NMR metabolites. Additionally, an aronia infusion (AI) arm, matching the anthocyanin content and profile of the RFA, was included. A 6-week, randomized, parallel study was conducted in hypercholesterolemic subjects (<em>n</em> = 121). Anthocyanin-rich products (RFA and AI) decreased LDLc; ApoB; total, large, and small LDL-P; LDL size; TG/HDL ratio; and large TRL, <em>versus</em> WFA. All treatments significantly decreased HDLc, ApoA1, and total HDL-P, with the most significant reductions after RFA treatment. RFA significantly decreased large HDL-P compared to WFA and AI, while medium HDL-P decreased significantly after AI compared to WFA. Anthocyanin-rich products decreased GlycA and alanine and increased acetoacetate <em>versus</em> WFA. WFA and RFA decreased plasma citrate <em>versus</em> AI. Thus, anthocyanin-rich products provided greater protection against CVD risk than WFA.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 6","pages":" 2279-2290"},"PeriodicalIF":5.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fo/d4fo02949f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}