Food & Function最新文献

{"title":"Correction: Effects of the DailyColors™ polyphenol supplement on serum proteome, cognitive function, and health in older adults at risk of cognitive and functional decline","authors":"Mary O'Leary, Joanna L. Bowtell, Megan Richards, Esra Bozbaş, Abbie Palmer, Kate Stych, Monica Meng, Adam Bloomfield, Lauren Struszczak, Jack Pritchard, Celeste Lugtmeijer, George Vere, Raif Yücel, Ana Rodriguez-Mateos, Zicheng Zhang, Jonathan C. Y. Tang, Clive Ballard and Anne Corbett","doi":"10.1039/D5FO90052B","DOIUrl":"10.1039/D5FO90052B","url":null,"abstract":"<p >Correction for ‘Effects of the DailyColors™ polyphenol supplement on serum proteome, cognitive function, and health in older adults at risk of cognitive and functional decline’ by Mary O'Leary <em>et al.</em>, <em>Food Funct.</em>, 2025, <strong>16</strong>, 4505–4520, https://doi.org/10.1039/d4fo06259k.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 13","pages":" 5652-5652"},"PeriodicalIF":5.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fo/d5fo90052b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The preventive effect of solubilized sturgeon oil on dextran sulfate sodium-induced ulcerative colitis via inflammation attenuation and intestinal microbiota regulation†","authors":"Ji-Hyun Park, Yong-Kwang Lee, Chong-Kil Lee, Hee-Sung Lee, Jeong-Hwan Kim, Min-Hyeok Lee, Yu-Jin Seo, Hwangeui Cho and Chan-Su Park","doi":"10.1039/D5FO01038A","DOIUrl":"10.1039/D5FO01038A","url":null,"abstract":"<p >Ulcerative colitis (UC), an inflammatory bowel disease, causes inflammation in the colonic mucosa. The pathogenesis of UC is closely linked to abnormalities in the gastrointestinal microbiota and immune response. Current treatments for UC primarily alleviate symptoms but are associated with several drawbacks, particularly with prolonged use. Therefore, there is a crucial need to explore novel treatment strategies. Solubilized sturgeon oil (SSO) has gained prominence for its anti-inflammatory effects in various contexts; however, its efficacy in UC has not yet been investigated. In this study, we investigated the preventive effects and underlying mechanisms of SSO in a dextran sulfate sodium-induced UC model. Oral administration of SSO significantly alleviated colitis severity by improving weight, disease activity index, and colon length. Moreover, SSO significantly downregulated the expression of interleukin (IL)-1β, IL-6, tumor necrosis factor-alpha, cyclooxygenase-2, and nitric oxide synthase, while enhancing the expression of tight junction-related proteins such as occludin and ZO-1 in colonic tissues. Additionally, SSO inhibited the activation of lipopolysaccharide-stimulated dendritic cells and macrophages and induced alterations in the gut microbiota, with increased <em>Firmicutes</em> and decreased <em>Bacteroidetes</em> abundances. Furthermore, colitis and gastrointestinal inflammation were alleviated in recipient mice that received fecal transplants from SSO-treated mice. These findings indicate that SSO is a promising natural therapeutic agent for preventing colitis by regulating the gastrointestinal microbiota and suppressing the hyperactivation of myeloid cells.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 13","pages":" 5421-5434"},"PeriodicalIF":5.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bifidobacterium breve M-16V alleviates cow's milk allergy by regulating the gut microbiota and metabolites in human microbiota-associated mice†","authors":"Huming Shao, Fangfang Min, Tianliang Bai, Yan Liu, Shuangyan Zheng, Yong Wu, Can Di, Min Lin, Xin Li and Hongbing Chen","doi":"10.1039/D5FO02012C","DOIUrl":"10.1039/D5FO02012C","url":null,"abstract":"<p >Cow's milk allergy (CMA) is one of the most common food allergies, especially in infants and young children. Growing evidence from animal studies has shown that some specific probiotics can alleviate CMA, but clinical evidence remains insufficient due to certain limitations. In the present study, we transplanted fecal material from three CMA children into antibiotic-pretreated mice (hum-CMA mice) to mimic the intestinal microecology of allergic individuals, followed by allergen sensitization and <em>Bifidobacterium breve</em> (<em>B</em>. <em>breve</em>) M-16V intervention. Our results showed that <em>B</em>. <em>breve</em> M-16V effectively ameliorated CMA symptoms and allergy-related indicators in hum-CMA mice. Moreover, <em>B</em>. <em>breve</em> M-16V differentially affected the composition of intestinal microbes, but the abundance of beneficial bacteria, such as short-chain fatty acid-producing bacteria, was consistently elevated in all three groups of hum-CMA mice. Subsequent untargeted metabolomics analyses revealed that <em>B</em>. <em>breve</em> M-16V improved the pattern of serum metabolites, and these differential metabolites were mainly involved in glutathione metabolism, glycerophospholipid metabolism, and tryptophan metabolism. All the findings indicate that <em>B</em>. <em>breve</em> M-16V can alleviate the anaphylactic reaction in hum-CMA mice by regulating the intestinal microbiota and metabolites, providing a valuable scientific basis for the clinical application of probiotics in food allergy.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 14","pages":" 5885-5899"},"PeriodicalIF":5.1,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation with beetroot modulates gut microbiota-derived diacylglycerol biosynthesis to enhance anti-tumor immunity in MMTV-PyMT (FVB) mice†","authors":"Jie Fu, Jianglin Chen, Jianyu Ni, Xia Liu, Qinghua Yao, Qiyang Shou and Huiying Fu","doi":"10.1039/D5FO01116G","DOIUrl":"10.1039/D5FO01116G","url":null,"abstract":"<p >Recent studies have established a strong connection between gut microbiota and the tumor immune microenvironment. Beetroot (BRT) has emerged as a promising functional food for cancer prevention and treatment, although its specific effects on breast cancer remain unclear. This study investigates the impact of BRT on polyomavirus middle T antigen (PyMT)-induced breast cancer by examining the gut microbiome, metabolites, and immune responses. Dietary supplementation of 80 g BRT powder per 1000 g of standard diet (8% w/w, 80 g per kg) significantly inhibited tumor growth and metastasis in mouse mammary tumor virus (MMTV)-PyMT (FVB) mice, while simultaneously enhancing anti-tumor immunity. 16S rRNA sequencing revealed that BRT supplementation altered the gut microbiota, notably increasing the abundance of <em>Lachnospiraceae</em> and <em>Ruminococcaceae</em>. An antibiotic (ABX) experiment confirmed that BRT restores gut microbiota balance, thereby enhancing anti-tumor immunity. Metabolomics and ELISA assays demonstrated that BRT regulates diacylglycerol (DAG) biosynthesis through microbiota modulation. Notably, diacylglycerol kinase <em>ζ</em> (DGK<em>ζ</em>) inhibitors enhance CD8+ T cell responses by increasing DAG levels, thereby activating Mitogen-Activated Protein Kinase (MAPK) and Protein Kinase C (PKC) signaling pathways. Collectively, our findings suggest that microbiota-derived DAG plays a key role in BRT-mediated enhancement of anti-tumor immunity.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 12","pages":" 5166-5187"},"PeriodicalIF":5.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human milk oligosaccharides mitigate infant diarrhea and anxiety-like behaviors via gut microbiota modulation in an EPEC O127 infection model†","authors":"Wenxiu Wang, Zhuo Liu, Ying He, Xue Wang, Jiabin Ma, Jingyi Ma, Pengfei Chang, Huidong Huang, Tian Yuan, Rui Guo, Yibing Ning and Zhigang Liu","doi":"10.1039/D5FO01854D","DOIUrl":"10.1039/D5FO01854D","url":null,"abstract":"<p >Infant diarrhea remains a leading cause of morbidity and mortality worldwide. Although human milk oligosaccharides (HMOs) have been clinically linked to reduced pathogen colonization in mice, their preventive effects on infant diarrhea and the underlying mechanisms remain unclear. In this study, one-week-old diarrhea C57BL/6J mice were induced with enteropathogenic <em>Escherichia coli</em> O127 (EPEC O127) to establish an infant diarrhea model. Meanwhile, the mice received HMOs containing 2′-fucosyllactose (2′-FL, 1 g kg<small>⁻¹</small>) and/or lacto-<em>N</em>-neotetraose (LNnT, 0.3 g kg<small>⁻¹</small>) for three weeks. The results showed that HMOs significantly alleviated diarrhea symptoms, mitigated anxiety-like behaviors, reduced colon inflammation, and restored gut barrier integrity. Notably, HMOs reshaped the gut microbiota by increasing the relative abundance of short-chain fatty acid (SCFA)-producing bacteria (<em>e.g.</em>, <em>Lacrimispora</em>), thereby elevating fecal SCFA levels while decreasing <em>Escherichia</em> and other harmful taxa. These findings suggest that HMOs can effectively relieve EPEC-induced infant diarrhea by modulating the composition and metabolic function of the gut microbiota. Overall, this study highlights the potential of HMOs as a dietary intervention for alleviating infant diarrhea and provides new insights into their application in infant health.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 14","pages":" 5757-5770"},"PeriodicalIF":5.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vanillin alleviates oxidative stress-mediated neuronal pyroptosis induced in rats by isoproterenol via SIRT1/NOX4/ROS/TXNIP/NLRP3 signaling pathway†","authors":"Asmaa M. Abdelghafour, Mohamed Mahrous and Mahmoud E. Zaher","doi":"10.1039/D4FO06458E","DOIUrl":"10.1039/D4FO06458E","url":null,"abstract":"<p >Neurodegenerative diseases (NDs) are considered a worldwide health concern that influences a large portion of the aging population. Recently, oxidative stress-mediated pyroptosis has emerged as an innovative therapeutic approach for many NDs including Alzheimer's disease (AD). Therefore, we aimed to examine the potential neuronal anti-pyroptotic activity of vanillin (VA) and to investigate the molecular mechanisms through which VA may ameliorate brain injury induced in rats including its effects on oxidative stress and pyroptosis, which play major roles in the pathogenesis of several NDs including AD. Neurotoxicity was induced in rats by two subcutaneous injections of isoproterenol (ISP) (100 mg kg<small>⁻¹</small>). ISP intervention lasted only two days, where the interval between each injection was 24 hours. After that, the rats were treated with VA (100 mg kg<small>⁻¹</small> day<small>⁻¹</small>, orally <em>via</em> gavage) for four weeks. ISP provoked neuronal oxidative stress that was characterized by elevated brain contents of reactive oxygen species (ROS), nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) and malonaldehyde (MDA) along with decreased brain contents of sirtuin 1 (SIRT1), glutathione (GSH) and superoxide dismutase (SOD). Furthermore, ISP increased the brain mRNA levels of thioredoxin-interacting protein (<em>TXNIP</em>), nod-like receptor-pyrin domain-containing 3 (<em>NLRP3</em>), apoptosis-associated speck-like protein containing a CARD (<em>ASC</em>), and <em>caspase-1</em> in addition to the brain contents of TXNIP, NLRP3, ASC, cleaved caspase-1, gasdermin D-N terminal (GSDMD-N), interleukin-18 (IL-18) and interleukin-1β (IL-1β), resulting in pyroptosis. Treatment with VA ameliorated ISP-induced oxidative stress, pyroptosis and histopathological changes <em>via</em> SIRT1/NOX4/ROS/TXNIP/NLRP3 signaling. Our findings suggest a novel therapeutic value for VA in ameliorating neurotoxicity <em>via</em> targeting oxidative stress-mediated pyroptosis.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 13","pages":" 5312-5325"},"PeriodicalIF":5.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PhenolQuest: a new reference FFQ for a more precise and comparable assessment of (poly)phenol intake in Europe†","authors":"Dorian Teissandier, Charlotte Gillet, Soline Chaumont, Sylvie Mercier, Marie Lefebvre, Wiame Lasri, Aïcha Djama Guedi, Marie Taillandier, Nathalie Hanet, Liliana Jimenez, Laetitia Demaretz, Christine Morand and Claudine Manach","doi":"10.1039/D4FO06326K","DOIUrl":"10.1039/D4FO06326K","url":null,"abstract":"<p >(Poly)phenols represent a large class of bioactive compounds present in our diet. An increasing number of epidemiological, clinical and pre-clinical studies are demonstrating the beneficial effects of (poly)phenols on various health parameters, parlicularly in relation to the prevention of cardiometabolic diseases. However, fully understanding their potential in preventive nutrition requires significant improvements in the assessment of individuals’ exposure to a variety of (poly)phenols. One of the current limitations is the use of standard Food Frequency Questionnaires (FFQs) that are not specifically designed for that purpose and as a consequence lack precision and accuracy for the assessment of (poly)phenol intake. We developed the FFQ PhenolQuest, specially designed to assess with a high level of details the dietary intake of 120 (poly)phenols belonging to all main families, in populations following a European-style diet. It includes 188 foods with a number of additional variations (<em>n</em> = 75). Special effort was made to optimize the questionnaire's design and usability. In particular, the consumption frequency options were expanded compared to the standard FFQ and tailored to the different food groups. An application test conducted on a French population panel of 110 subjects demonstrated PhenolQuest's ability to capture the diversity of (poly)phenol source consumption patterns. PhenolQuest is shared in integrality for free use and further validation by the community. This article presents the challenges and strategies used for its development, as well as its scope of use.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 14","pages":" 5791-5808"},"PeriodicalIF":5.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fo/d4fo06326k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Avenanthramide C from underutilized oat glumes exhibits dual anti-oxidant and anti-thrombotic effects in oxidative damage and thrombosis models†","authors":"Li-Wei Wang, Jiang-Hong An, Meng-Ran Zhao, Li-Li Jiang, Hua Sun, Fang Liu, Ya-Ru Tang, Xiao-Qi Ren, Xing-Ran Dong, Jing-Da Yuan, Jiang-Feng He and Jie Zhao","doi":"10.1039/D5FO00366K","DOIUrl":"10.1039/D5FO00366K","url":null,"abstract":"<p >Avenanthramides (Avns), particularly Avn-C, are unique phenolic compounds found in oats, known for their anti-oxidant and anti-inflammatory effects. This study evaluated Avn-C's bioactivity using oxidative damage models in HUVECs induced by <em>t</em>-BOOH and thrombosis models in zebrafish induced by phenylhydrazine (PHZ). Avn-C exhibited potent antioxidant activity, with an IC<small>₅₀</small> value of 7.38 μg mL<small>⁻¹</small> for DPPH radical scavenging. It effectively reduced <em>t</em>-BOOH-induced apoptosis, decreased reactive oxygen species (ROS) levels, inhibited IL-17 signaling, and lowered pro-inflammatory cytokine release, thus mitigating oxidative stress-induced endothelial dysfunction. <em>In vivo</em>, Avn-C significantly alleviated thrombosis symptoms induced by PHZ in zebrafish. To explore enhanced bioactivity applications, we quantified Avns in different oat plants’ parts and growth stages using UHPLC-QqQ-MS. Notably, the glume, often overlooked, contains significantly higher levels of these compounds than the traditionally emphasized oat bran. These findings enhance our understanding of Avn distribution and emphasize the innovative development value of oat glumes.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 13","pages":" 5380-5392"},"PeriodicalIF":5.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal high-fat diet during lactation reduces sialylated milk oligosaccharides and shapes early-life microbiota in rat offspring†","authors":"Lucie Marousez, Marko Verce, Laure Dubernat, Thameur Rakza, Patrice D. Cani, Frédéric Gottrand, Delphine Ley, Amandine Everard, Delphine Eberlé and Jean Lesage","doi":"10.1039/D5FO00559K","DOIUrl":"10.1039/D5FO00559K","url":null,"abstract":"<p >Human milk oligosaccharides (HMOs) promote the development of the infant gut microbiota, notably through the growth of beneficial bacteria. However, the interaction between maternal diet, HMOs and the offspring's gut microbiota remains incompletely understood. We hypothesize that a high-fat (HF) diet influences the composition of HMOs and shapes gut microbiota in offspring. In lactating rat mothers fed with a HF or a low-fat (LF) diet, we measured at mid-lactation, the levels of milk sialylated oligosaccharides (SL-MOs) and the expression of enzymes for synthesizing SL-MOs in the mammary gland. In rat pups from LF or HF mothers, we analysed the caecal microbiota and measured levels of faecal short-chain fatty acids (SCFAs). In addition, in humans, we quantified 24 HMOs in the colostrum of lean and obese mothers. In rat mothers, HF feeding significantly reduced the breast milk 6′SL level but not the 3′SL one and increased the gene-expression level of the St3Gal1 enzyme in the mammary gland. In HF rat pups, the caecal microbiota was drastically modified with increases in α- and β-diversities as well as in the abundance of <em>Parabacteroides</em> and Lachnospiraceae. Levels of faecal SCFAs including acetate, butyrate, isobutyrate, valerate and isovalerate were also increased in HF pups. In humans, maternal obesity only slightly increased DFLNHa concentration in the colostrum. In conclusion, our data suggest that a hyperlipidic diet during lactation may reduce the level of sialylated oligosaccharides in breast milk and alter the abundance of several groups of bacteria in the infant's gut. We also demonstrated that, in humans, obesity did not highly affect levels of HMOs in the colostrum reinforcing previous findings suggesting that being overweight does not influence drastically the HMO profile in breast milk.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 12","pages":" 5123-5132"},"PeriodicalIF":5.1,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fo/d5fo00559k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preventive and controlling effects of N-acetylneuraminic acid in regulating glucose and lipid metabolism disorders via the gut-liver axis in high-fat diet mice","authors":"Wei Zhang, Linlin Zhou, Xinyuan Huang, Xinning Zhao, Hanying Zheng, Dongbei Guo, Xiaoxuan Chen, Lili Pan, Yahui Li and Hongwei Li","doi":"10.1039/D5FO00484E","DOIUrl":"10.1039/D5FO00484E","url":null,"abstract":"<p >A high-fat diet may disrupt sialic acid homeostasis indirectly by altering the gut microbiota and metabolic pathways. Sialic acid interventions have immune-regulatory effects and can improve gut health, but their impact on glucose and lipid metabolism disorders, both before and after obesity induced by a high-fat diet, remains unclear. This study used two models: a preventive experiment (intervention during high-fat diet feeding) and a control experiment (intervention after obesity induction). Each model consisted of a blank control group, a high-fat diet control group, and three <em>N</em>-acetylneuraminic acid (Neu5Ac) intervention groups (low, medium, and high doses), with 12 mice per group. The results showed that both intervention models effectively improved glucose tolerance, and reduced insulin levels, although no significant dose–response relationship was observed. Omics analysis revealed that the intervention increased the abundance of gut microbiota associated with energy metabolism and affected energy metabolism, immune response, and oxidative stress-related signaling pathways in the liver and colon. In the control experiment, inflammation marker levels correlated with improvements in the gut microbiota. Furthermore, the intervention significantly altered the abundance of microbiota linked to glycosylation signaling and metabolic regulation. These findings suggest that Neu5Ac intervention improves gut microbiota structure and function, stabilizes glycan structures, and reduces immune-inflammatory signaling in the gut and liver, thereby lowering systemic inflammation. This helps prevent and control glucose and lipid metabolism disorders induced by a high-fat diet. Notably, the intervention showed stronger effects after obesity had developed due to the high-fat diet.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" 12","pages":" 5151-5165"},"PeriodicalIF":5.1,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/fo/d5fo00484e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}