Diagnostic and clinical immunology最新文献_第5页

Correlation between ANA determinations on tissue substrate, WiL-2 cell substrate, and precipitin antibody by double diffusion. 双扩散法测定组织底物、will -2细胞底物与沉淀抗体的相关性。

Diagnostic and clinical immunology Pub Date : 1987-01-01

J S Deng, L W Bair, T A Medsger, R A Sontheimer

引用次数: 0

Human immunoglobulin subclasses. 人免疫球蛋白亚类。

Diagnostic and clinical immunology Pub Date : 1987-01-01

D E Normansell

引用次数: 0

Specificity of the hydrochloric-acid-modified Crithidia luciliae immunofluorescence assay for detection of antibody to native DNA. 盐酸修饰的水仙免疫荧光法检测天然DNA抗体的特异性。

Diagnostic and clinical immunology Pub Date : 1987-01-01

T Henderson, T A Medsger, R D Sontheimer, C E Harmon, J S Deng

引用次数: 0

Difficulties associated with serological diagnosis of Toxoplasma gondii infections. 刚地弓形虫感染血清学诊断的相关困难。

Diagnostic and clinical immunology Pub Date : 1987-01-01

D A Fuccillo, D L Madden, N Tzan, J L Sever

{"title":"Difficulties associated with serological diagnosis of Toxoplasma gondii infections.","authors":"D A Fuccillo, D L Madden, N Tzan, J L Sever","doi":"","DOIUrl":"","url":null,"abstract":"Physicians often rely on serology to help determine whether a patient has had a recent infection with Toxoplasma gondii and as an aid in estimating the possible teratogenic effect on the fetus. For this reason the diagnostic laboratory should take every precaution to avoid misleading results. The best serological analysis is based on a rise in IgG titer with two appropriately spaced serum samples. Also, the presence of a high IgM titer in one serum sample is generally considered to be good evidence that infection has occurred recently. The indirect fluorescent antibody (IFA) test has been the most widely used test for detection of IgG or IgM. Recently enzyme-linked immunosorbent assays (ELISA) have also been developed for this purpose. In this study we reaffirm that false IgM positive results can occur with these tests because of the presence of rheumatoid factor in serum, and false negative results can also occur because of competitive inhibition by specific IgG. We show that a preabsorption of serum with a Staphylococcus/Streptococcus preparation (Staffinoc, MA Bioproducts, Walkersville, MD) removes IgG and IgA and eliminates many of the false reactions. We have also found that elevated levels of specific IgM can persist for at least several years in some women. This suggests that the presence of IgM alone is not always an indication of recent infection.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 1","pages":"8-13"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14424844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of human plasma-associated hepatitis B surface antigens by monoclonal antibodies (MAb): the same MAb can be used as both capture and tracer antibody. 用单克隆抗体(MAb)检测人血浆相关乙型肝炎表面抗原:同一MAb既可作为捕获抗体，也可作为示踪抗体。

Diagnostic and clinical immunology Pub Date : 1987-01-01

K F Chi, M D Scanlon, R Henkel, G Dreesman, J S Seo, J M Bowen, J C Chan

{"title":"Detection of human plasma-associated hepatitis B surface antigens by monoclonal antibodies (MAb): the same MAb can be used as both capture and tracer antibody.","authors":"K F Chi, M D Scanlon, R Henkel, G Dreesman, J S Seo, J M Bowen, J C Chan","doi":"","DOIUrl":"","url":null,"abstract":"We have produced a monoclonal antibody (MAb), designated 11G12, of IgG1 isotype that recognizes group-specific epitopes of the HBsAg. It was demonstrated that the same MAb 11G can be used effectively as both the capture and tracer antibody to detect HBsAg in human plasma samples, using sandwich radioimmune assays (11G-11G sRIA) and sandwich avidin-biotin types of enzymatic assays (11G-11G sABC). The sensitivities for 11G-11G sRIA and 11G-11G sABC is 2.5 ng/ml and 5.0 ng/ml of HBsAg, respectively. Our data suggest that sufficient numbers of the same epitope exist on the HBsAg so that the use of the same MAb as both capture and tracer antibody does not reduce the sensitivity of the assays. The use of MAb 11G as both capture and tracer also minimizes the \"hook effect\" often encountered in testing high concentrations of plasma HBsAg by other assays. We also experimented with a sandwich RIA in which MAb 11G was used as the capture and polyclonal anti-HBs antibody as the tracer and found that such a combination increased the sensitivity of detection to 1.25 ng/ml of HBsAg.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 2","pages":"91-9"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13588951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of antibodies adsorbed on the DNA immunoadsorbent, agarose poly-L-lysine-DNA. 抗体在DNA免疫吸附剂琼脂糖聚赖氨酸DNA上的吸附特性。

Diagnostic and clinical immunology Pub Date : 1987-01-01

P A Belmonte, L B Keil, V A DeBari

{"title":"Characteristics of antibodies adsorbed on the DNA immunoadsorbent, agarose poly-L-lysine-DNA.","authors":"P A Belmonte, L B Keil, V A DeBari","doi":"","DOIUrl":"","url":null,"abstract":"Agarose-poly-L-lysine (Ag-(lys)n-DNA) has been used to bind DNA for assay of anti-DNA antibodies (ab). In this work, an algorithmic approach has been used to classify antinuclear ab (ANA) as being directed against native DNA (dsDNA), denatured DNA (ssDNA), DNA-protein complexes (deoxyribonucleoprotein; DNP), and against antigens which are independent of DNA (iDNA). These ab were subjected to Ag-(lys)n-DNA, and the selectivity of this adsorbent for the various specificities of ab was determined. The DNA on the columns was left untreated or treated with S1 nuclease, this being effected either by treating the DNA prior to introducing it onto the columns or by adding S1 nuclease to the columns after the DNA was bound. Ag-(lys)n-DNA adsorbs ab directed against ssDNA and DNP as well as ab to dsDNA; iDNA ab are not adsorbed. S1 nuclease treatment does not effectively remove ssDNA regions from the Ag-(lys)n-DNA, but it does result in the abolition of the adsorption of a population of ab which are in the anti-DNP sera and contribute to the total ANA load. While anti-iDNA ab are not adsorbed onto the columns, they do contribute to the ANA titer, unlike anti-ssDNA ab which are adsorbed onto the Ag-(lys)n-DNA but do not contribute to the ANA titer. We conclude that Ag-(lys)n-DNA bears antigenic sites for dsDNA, ssDNA, and DNP ab and suggest that our understanding of the characteristic ab-binding profile of this versatile immunoadsorbent may have applications in the study of autoimmune diseases.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 3","pages":"144-50"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13592990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interleukin-1 activity from human cord blood monocytes. 人脐带血单核细胞白细胞介素-1活性。

Diagnostic and clinical immunology Pub Date : 1987-01-01

R W Wilmott, M C Harris, K M Haines, S D Douglas

引用次数: 0

Flow cytometric lymphocyte immunophenotyping in homosexual men with the persistent generalized lymphadenopathy syndrome: a longitudinal study of lymph nodes and blood. 男同性恋者持续性全身性淋巴结病综合征的流式细胞淋巴细胞免疫分型:淋巴结和血液的纵向研究。

Diagnostic and clinical immunology Pub Date : 1987-01-01

R R Turner, D C Boone, A M Levine, P W Nichols, J W Parker

引用次数: 0

Increased levels of soluble interleukin-2 receptor in the serum of patients with human immunodeficiency virus infection. 人类免疫缺陷病毒感染患者血清中可溶性白介素-2受体水平升高。

Diagnostic and clinical immunology Pub Date : 1987-01-01

G Pizzolo, F Vinante, A Sinicco, M Chilosi, C Agostini, A Perini, B Zuppini, G Semenzato, L Battistella, R Foa

{"title":"Increased levels of soluble interleukin-2 receptor in the serum of patients with human immunodeficiency virus infection.","authors":"G Pizzolo, F Vinante, A Sinicco, M Chilosi, C Agostini, A Perini, B Zuppini, G Semenzato, L Battistella, R Foa","doi":"","DOIUrl":"","url":null,"abstract":"The serum levels of a soluble form of the interleukin-2 receptor (sIL-2R) were investigated in 92 patients with human immunodeficiency virus (HIV) infection, ranging from asymptomatic cases to full-blown AIDS. Increased values were found in 69.5% of cases. The overall mean was significantly higher (p less than 0.001) in HIV-infected patients (mean +/- SD = 709.3 +/- 369.4 U/ml) than in the seronegative risk group controls (383.9 +/- 140.5) and normal controls (256.4 +/- 114.5). No major differences were found among the patient groups (asymptomatic infection, persistent generalized lymphadenopathy, symptomatic infection, and full-blown AIDS). These data suggest that the measurement of serum sIL-2R levels may represent a useful biological tool for evaluating T-cell activation phenomena occurring in HIV infection. Since the soluble interleukin-2 receptor maintains the capacity of binding interleukin-2, the increased levels found in HIV infection may play a contributory role towards the in vitro and in vivo impairment of a number of interleukin-2-dependent functions described in this disease. On clinical grounds, the excess of sIL-2R could help to explain the lack of therapeutic effect and little immunological variations following the in vivo administration of interleukin-2.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 4","pages":"180-3"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14253603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CALLA-negative, TdT- and CD7-positive acute lymphoblastic leukemia: a phenotype associated with poor prognosis. calla阴性、TdT-和cd7阳性的急性淋巴细胞白血病:一种与不良预后相关的表型

Diagnostic and clinical immunology Pub Date : 1987-01-01

G De Rossi, M A Aloe Spiriti, A Cafolla, R Gastaldi, F Lo Coco, M Lopez, M Luciani, D Pasqualetti, A M Testi, F Mandelli

引用次数: 0