Specificity of the hydrochloric-acid-modified Crithidia luciliae immunofluorescence assay for detection of antibody to native DNA.

Diagnostic and clinical immunology Pub Date : 1987-01-01
T Henderson, T A Medsger, R D Sontheimer, C E Harmon, J S Deng
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引用次数: 0

Abstract

The Crithidia luciliae immunofluorescence (CLIF) assay is widely used to detect antibodies to native dsDNA in the diagnosis and management of systemic lupus erythematosus (SLE). However, sera from patients with SLE, rheumatoid arthritis, systemic sclerosis, drug-induced lupus erythematosus, and Sjogren's syndrome have given false-positive CLIF results. The frequency was 5% for SLE, 16% for drug-induced LE, and 5% for rheumatoid arthritis. Such false positivity was effectively eliminated by pretreatment of Crithidia luciliae smears with 0.1 N HCl. Hydrochloric acid pretreatment of Crithidia luciliae smears renders the CLIF test more specific for the detection of anti-dsDNA antibodies, without sacrificing its sensitivity and specificity. In the future, modification of routine Crithidia luciliae immunofluorescence with 0.1 N HCl pretreatment is recommended.

盐酸修饰的水仙免疫荧光法检测天然DNA抗体的特异性。
在系统性红斑狼疮(SLE)的诊断和治疗中,黄貂草免疫荧光法(CLIF)被广泛用于检测天然dsDNA抗体。然而,SLE、类风湿关节炎、系统性硬化症、药物性红斑狼疮和干燥综合征患者的血清CLIF结果呈假阳性。SLE为5%,药物性LE为16%,类风湿关节炎为5%。用0.1盐酸对栀子涂片进行预处理,可有效消除假阳性。在不牺牲其敏感性和特异性的前提下,对水仙涂片进行盐酸预处理,可提高CLIF检测抗dsdna抗体的特异性。今后推荐采用0.1盐酸预处理对常规水仙免疫荧光进行修饰。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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