Characteristics of antibodies adsorbed on the DNA immunoadsorbent, agarose poly-L-lysine-DNA.

Diagnostic and clinical immunology Pub Date : 1987-01-01
P A Belmonte, L B Keil, V A DeBari
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Abstract

Agarose-poly-L-lysine (Ag-(lys)n-DNA) has been used to bind DNA for assay of anti-DNA antibodies (ab). In this work, an algorithmic approach has been used to classify antinuclear ab (ANA) as being directed against native DNA (dsDNA), denatured DNA (ssDNA), DNA-protein complexes (deoxyribonucleoprotein; DNP), and against antigens which are independent of DNA (iDNA). These ab were subjected to Ag-(lys)n-DNA, and the selectivity of this adsorbent for the various specificities of ab was determined. The DNA on the columns was left untreated or treated with S1 nuclease, this being effected either by treating the DNA prior to introducing it onto the columns or by adding S1 nuclease to the columns after the DNA was bound. Ag-(lys)n-DNA adsorbs ab directed against ssDNA and DNP as well as ab to dsDNA; iDNA ab are not adsorbed. S1 nuclease treatment does not effectively remove ssDNA regions from the Ag-(lys)n-DNA, but it does result in the abolition of the adsorption of a population of ab which are in the anti-DNP sera and contribute to the total ANA load. While anti-iDNA ab are not adsorbed onto the columns, they do contribute to the ANA titer, unlike anti-ssDNA ab which are adsorbed onto the Ag-(lys)n-DNA but do not contribute to the ANA titer. We conclude that Ag-(lys)n-DNA bears antigenic sites for dsDNA, ssDNA, and DNP ab and suggest that our understanding of the characteristic ab-binding profile of this versatile immunoadsorbent may have applications in the study of autoimmune diseases.

抗体在DNA免疫吸附剂琼脂糖聚赖氨酸DNA上的吸附特性。
琼脂糖-聚l -赖氨酸(Ag-(lys)n-DNA)已被用于结合DNA检测抗DNA抗体(ab)。在这项工作中,使用了一种算法方法将抗核抗体(ANA)分类为针对天然DNA (dsDNA),变性DNA (ssDNA), DNA-蛋白质复合物(脱氧核糖核蛋白;DNP),并针对独立于DNA的抗原(iDNA)。将这些抗体置于Ag-(lys)n-DNA中,并测定了这种吸附剂对各种抗体的选择性。柱上的DNA未经处理或用S1核酸酶处理,这可以通过在将DNA引入柱上之前处理DNA或在DNA结合后将S1核酸酶添加到柱上来实现。Ag-(lys)n-DNA吸附针对ssDNA和DNP的ab,以及针对dsDNA的ab;dna ab不被吸附。S1核酸酶处理不能有效地去除Ag-(lys)n-DNA上的ssDNA区域,但它确实导致了抗dnp血清中ab群吸附的消除,并有助于总ANA负荷。虽然抗idna抗体不被吸附到柱上,但它们确实有助于抗ANA滴度,不像抗ssdna抗体被吸附到Ag-(lys)n-DNA上,但不有助于抗ANA滴度。我们得出结论,Ag-(lys)n-DNA具有dsDNA、ssDNA和DNP抗体的抗原位点,并提示我们对这种多功能免疫吸附剂的特异性抗体结合谱的理解可能在自身免疫性疾病的研究中具有应用价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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