Diagnostic and clinical immunology最新文献_第7页

A simultaneous flow cytometric measurement of neutrophil phagocytosis and oxidative burst in whole blood. 流式细胞术同时测定全血中性粒细胞吞噬和氧化破裂。

Diagnostic and clinical immunology Pub Date : 1987-01-01

L S Trinkle, S R Wellhausen, K R McLeish

引用次数: 0

Measurement of antineutrophil antibodies by flow cytometry: simultaneous detection of antibodies against monocytes and lymphocytes. 流式细胞术测定抗中性粒细胞抗体:同时检测抗单核细胞和淋巴细胞的抗体。

Diagnostic and clinical immunology Pub Date : 1987-01-01

J P Robinson, R E Duque, L A Boxer, P A Ward, J L Hudson

{"title":"Measurement of antineutrophil antibodies by flow cytometry: simultaneous detection of antibodies against monocytes and lymphocytes.","authors":"J P Robinson, R E Duque, L A Boxer, P A Ward, J L Hudson","doi":"","DOIUrl":"","url":null,"abstract":"We describe a flow cytometric technique which detects the presence of antineutrophil antibodies (NABs) in human serum. The technique provides a qualitative as well as a semiquantitative screen and provides an excellent method for monitoring the presence of antineutrophil antibodies in patients with suspected autoimmune neutropenia. Using light-scatter gating, individual populations consisting of neutrophils, monocytes, and lymphocytes can be examined simultaneously for the presence of antibody. The methodology utilizes an indirect immunofluorescence technique with FITC-labeled goat antihuman F(ab')2 antibody and fixed leukocyte suspensions. Furthermore, by utilizing class-specific FITC-labeled second antibody, significant information can be ascertained regarding the class, cell specificity, and quantity of detected antibody. Formalin fixation of neutrophils prevented pinocytosis of the fluorochrome, significantly reducing background fluorescence. Twenty-five normal subjects provided baseline antibody levels for each class. Of 92 patients with suspected autoimmune neutropenia, 27 had class IgG alone and seven were positive for both IgG and IgM class NABs. During treatment, IgG levels varied. IgM NABs alone were detected in four patients. Fifty-four patients had undetectable antibody. Antibodies were detected against monocytes in several of the IgG-positive patients. Two sera contained both IgA and IgG NABs. One serum contained IgA and IgG antibodies against monocytes. No IgD antibodies were detected in any sera tested. Some sera tested contained antibodies against lymphocytes--however, only in those sera which also contained antibodies to other cell types.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 4","pages":"163-70"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14448668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complement activation in fetuses: assessment by the levels of complement components and split products in cord blood. 胎儿补体激活:通过脐带血中补体成分和分裂产物水平的评估。

Diagnostic and clinical immunology Pub Date : 1987-01-01

A Miyano, M Nakayama, T Fujita, H Kitajima, S Imai, A Shimizu

引用次数: 0

Ultrastructural morphometric analysis of nuclear contour irregularity in normal cord and adult blood: correlation with distinct lymphocyte subpopulations. 正常脐带血和成人血核轮廓不规则的超微结构形态计量学分析:与不同淋巴细胞亚群的相关性。

Diagnostic and clinical immunology Pub Date : 1987-01-01

C M Payne, M J Hicks, C G Bjore, R Kibler

{"title":"Ultrastructural morphometric analysis of nuclear contour irregularity in normal cord and adult blood: correlation with distinct lymphocyte subpopulations.","authors":"C M Payne, M J Hicks, C G Bjore, R Kibler","doi":"","DOIUrl":"","url":null,"abstract":"Cord blood lymphocytes obtained from ten healthy newborns and peripheral blood obtained from ten healthy adults were compared by using ultrastructural morphometric methods. Cord blood lymphocytes could be distinguished from adult lymphocytes on the basis of nuclear contour irregularity (form factor) and perimeter (P less than .001). The mean nuclear form factor (FF) value of the cord lymphocytes, as a group, was 0.603 +/- 0.034; the mean nuclear FF value of adult lymphocytes was 0.755 +/- 0.032. Lymphocyte subpopulations were quantitated by flow cytometric and immunofluorescence techniques and correlated with nuclear contour irregularity (mean FF values) by using linear regression analysis. Overall, the correlations between nuclear contour irregularity and the proportions of distinct lymphocyte subpopulations were opposite for cord and adult blood. Increased nuclear contour irregularity was associated with increased proportions of immature lymphocytes (non-T, non-B lymphocyte population) in the neonate and with increased proportions of T cells in the adult. Of the T cells, increased nuclear contour irregularity showed a better correlation with increased proportions of T helper cells compared with T suppressor cells. A plot of the distribution of FF values indicated two populations of lymphocytes having convoluted and nonconvoluted nuclei, respectively. No true Sézary cells could be identified in cord blood with ultrastructural morphometric criteria, despite the abundance of cerebriform mononuclear cells identified by visual inspection. The lymphocyte with the most irregular nuclear contour was found in an adult and had an FF value of 0.132 (13% of a circle). The contribution of this study to the diagnosis of cutaneous T cell lymphomas and the Sézary syndrome is discussed.","PeriodicalId":77705,"journal":{"name":"Diagnostic and clinical immunology","volume":"5 1","pages":"41-53"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14716724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monoclonal antibody recognizing a human neuroblastoma-associated antigen. 识别人神经母细胞瘤相关抗原的单克隆抗体。

Diagnostic and clinical immunology Pub Date : 1987-01-01

R G Smith, C P Reynolds

引用次数: 0

Receptors for mouse erythrocytes on human immature B lymphocytes: still a valuable tool in immunohaematology. 小鼠红细胞在人未成熟B淋巴细胞上的受体:在免疫血液学中仍然是一个有价值的工具。

Diagnostic and clinical immunology Pub Date : 1987-01-01

P Y Youinou, P M Lydyard

引用次数: 0