Analytical and quantitative cytology and histology最新文献_第6页

Brownian motion curve-based textural classification and its application in cancer diagnosis. 基于布朗运动曲线的纹理分类及其在癌症诊断中的应用。

Analytical and quantitative cytology and histology Pub Date : 2011-06-01

Muthu Rama Krishnan Mookiah, Pratik Shah, Chandan Chakraborty, Ajoy K Ray

{"title":"Brownian motion curve-based textural classification and its application in cancer diagnosis.","authors":"Muthu Rama Krishnan Mookiah, Pratik Shah, Chandan Chakraborty, Ajoy K Ray","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To develop an automated diagnostic methodology based on textural features of the oral mucosal epithelium to discriminate normal and oral submucous fibrosis (OSF).Study design: A total of 83 normal and 29 OSF images from histopathologic sections of the oral mucosa are considered. The proposed diagnostic mechanism consists of two parts: feature extraction using Brownian motion curve (BMC) and design ofa suitable classifier. The discrimination ability of the features has been substantiated by statistical tests. An error back-propagation neural network (BPNN) is used to classify OSF vs. normal.Results: In development of an automated oral cancer diagnostic module, BMC has played an important role in characterizing textural features of the oral images. Fisher's linear discriminant analysis yields 100% sensitivity and 85% specificity, whereas BPNN leads to 92.31% sensitivity and 100% specificity, respectively.Conclusion: In addition to intensity and morphology-based features, textural features are also very important, especially in histopathologic diagnosis of oral cancer. In view of this, a set of textural features are extracted using the BMC for the diagnosis of OSF. Finally, a textural classifier is designed using BPNN, which leads to a diagnostic performance with 96.43% accuracy. (Anal Quant","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 3","pages":"158-68"},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30191907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA damage in women with cervical neoplasia evaluated by DNA breakage detection-fluorescence in situ hybridization. DNA断裂检测-荧光原位杂交评价宫颈肿瘤妇女DNA损伤。

Analytical and quantitative cytology and histology Pub Date : 2011-06-01

Elva I Cortés-Gutiérrez, Martha I Dávila-Rodríguez, José Luís Fernandez, Carmen López-Fernández, Jaime Gosálvez

{"title":"DNA damage in women with cervical neoplasia evaluated by DNA breakage detection-fluorescence in situ hybridization.","authors":"Elva I Cortés-Gutiérrez, Martha I Dávila-Rodríguez, José Luís Fernandez, Carmen López-Fernández, Jaime Gosálvez","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To evaluate the association between the progressive stages of cervical dysplasia and DNA damage.Study design: A hospital-based, unmatched case-control study was performed. DNA damage levels in the cervical epithelial cells of 30 women (10 with low-grade squamous intraepithelial lesions [LSIL], 10 with high-grade SIL [HSILI, and 10 with no cervical lesions) were evaluated using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique. DNA damage levels were measured quantitatively using image analysis after whole-genomic DNA hybridization.Results: LSIL patients presented a hybridization signal 20 times greater than the signal in control individuals, which reflected the basal level of DNA damage detected, and HSIL patients showed 100 times the basal control signal. The Kruskal-Wallis test showed differences between both patient groups and the control and between the patients with LSIL and HSIL.Conclusion: The DBD-FISH technique is easy to apply to cervical scrapings and provides prompt results. Our findings confirm that the grade of a cervical lesion correlates with the degree of genomic instability.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 3","pages":"175-81"},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30191909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computer-aided diagnosis of melanocytic skin tumors by use of confocal laser scanning microscopy images. 激光共聚焦扫描显微图像在黑素细胞性皮肤肿瘤计算机辅助诊断中的应用。

Analytical and quantitative cytology and histology Pub Date : 2011-04-01

Marco Wiltgen, Marcus Bloice, Silvia Koller, Rainer Hoffmann-Wellenhof, Josef Smolle, Armin Gerger

{"title":"Computer-aided diagnosis of melanocytic skin tumors by use of confocal laser scanning microscopy images.","authors":"Marco Wiltgen, Marcus Bloice, Silvia Koller, Rainer Hoffmann-Wellenhof, Josef Smolle, Armin Gerger","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To check the applicability of machine learning algorithms for the computer-aided diagnosis of confocal laser scanning microscopy (CLSM) views of skin lesions.Study design: Features, based on spectral properties of the wavelet transform, are very suitable for the automatic analysis because architectural structures at different scales play an important role in diagnosis of CLSM views. The images are discriminated by several machine learning algorithms, based on Bayes-, tree-, rule-, function (numeric)-, and lazy-classifiers.Results: The function and lazy classifiers delivered best classification results. However, these algorithms deliver no information about the inference mechanism leading to the classification. The tree classifiers provided better results than the rule classifiers. To obtain more insight into the inference process, and to compare it with the diagnostic guidelines of the dermopathologists, we combined the advantages of tree, numerical, and rule classifiers and choose the classification and regression trees (CART) algorithm, which automatically generates accurate inferring rules. The classification results were relocated to the images by use of the inferring rules as diagnostic aid.Conclusion: The discriminated elements of the skin lesions images show tissue with features in good accordance with typical diagnostic CLSM features.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 2","pages":"85-100"},"PeriodicalIF":0.0,"publicationDate":"2011-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30191378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simplification of grading papillary urothelial neoplasia using a reduced set of diagnostic features. 用一组简化的诊断特征简化乳头状尿路上皮瘤的分级。

Analytical and quantitative cytology and histology Pub Date : 2011-04-01

Björn L Isfoss, Bernard Majak, Christer Busch, Geir J Braathen

{"title":"Simplification of grading papillary urothelial neoplasia using a reduced set of diagnostic features.","authors":"Björn L Isfoss, Bernard Majak, Christer Busch, Geir J Braathen","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To determine whether a reduced set of the histopathologic features used in internationally accepted classifications is capable of accurately grading papillary urothelial neoplasms (PUN).Study design: All surgical specimens from urinary bladders received during a 2-year period were reexamined by an expert uropathologist for assessing the accuracy of original nonexpert PUN grading and staging. Thirteen histopathologic features entailing 32 attributes were evaluated with regard to prediction of expert grade. Patients were followed for 35-59 months (mean, 47).Results: A total of 88 PUN specimens could be analyzed completely including follow-up specimens. Agreement between original and expert grade was 71% for low-grade and 87% for high-grade PUN, with overall kappa = 0.53. The histomorphologic features most predictive of expert grade were architectural disorder, variability of nuclear enlargement, and absence of umbrella cells. Neither individual histomorphologic attributes nor their combinations were as predictive of expert pathologist grade as original diagnoses.Conclusion: Improvements in PUN grading and prognostication are not likely to be accomplished by only reducing the number of histomorphologic features currently recommended by the World Health Organization and International Society of Urological Pathology.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 2","pages":"68-74"},"PeriodicalIF":0.0,"publicationDate":"2011-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30193987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Penile cancer: different histopathologic types with a critical clinical approach. 阴茎癌:不同的组织病理类型与关键的临床方法。

Analytical and quantitative cytology and histology Pub Date : 2011-04-01

Alessandra Filosa, Andrea Fabiani

引用次数: 0

Differential expression of estrogen and progesterone receptors in endometrial polyps and adjacent endometrium in postmenopausal women. 绝经后妇女子宫内膜息肉及邻近子宫内膜中雌激素和孕激素受体的差异表达。

Analytical and quantitative cytology and histology Pub Date : 2011-04-01

Soraia de Carvalho, Adriana Bittencourt Campaner, Sônia Maria Rolim Rosa Lima, Maria Antonieta Longo Galvão Silva, Paulo Augusto Ayroza Ribeiro

{"title":"Differential expression of estrogen and progesterone receptors in endometrial polyps and adjacent endometrium in postmenopausal women.","authors":"Soraia de Carvalho, Adriana Bittencourt Campaner, Sônia Maria Rolim Rosa Lima, Maria Antonieta Longo Galvão Silva, Paulo Augusto Ayroza Ribeiro","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To evaluate the immunoexpression of estrogen receptor (ER) and progesterone receptor (PR) in gland and stromal cells of endometrial polyps in postmenopausal women.Study design: Thirty postmenopausal patients underwent operative hysteroscopies because of benign endometrial polyps. The polyps were identified and subsequently completely removed. A section of normal-appearing endometrium adjacent to the polyp base was also obtained for the control group. The presence of ER and PR was investigated in the gland and stromal cells in the polyps and adjacent endometrium using immunohistochemistry. The slides were evaluated by semiquantitative analysis.Results: Endometrium and endometrial polyps showed a significantly higher proportion of positive cells in the glands than in the stroma for both ER (p < 0.000 and p < 0.000, respectively) and PR (p = 0.002 and p = 0.002, respectively). Polyps showed a significantly higher proportion of positive cells in glands and stroma than in the endometrium, concerning ER (p < 0.000 and p = 0.001, respectively) and also for PR (p = 0.021 and p = 0.008, respectively).Conclusion: Our data suggest that steroid receptors present a crucial role in the physiopathology of the endometrial polyps in postmenopausal women.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 2","pages":"61-7"},"PeriodicalIF":0.0,"publicationDate":"2011-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30193986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fractal and lacunar dimension of the chorionic villi of molar pregnancy. 磨牙妊娠绒毛膜绒毛的分形和腔隙维数。

Analytical and quantitative cytology and histology Pub Date : 2011-04-01

Sandeep Kumar Arora, Pranab Dey

{"title":"Fractal and lacunar dimension of the chorionic villi of molar pregnancy.","authors":"Sandeep Kumar Arora, Pranab Dey","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To explore the role of fractal geometry to differentiate molar pregnancies from normal chorionic villi.Study design: We selected at random 15 cases each of first-trimester normal chorionic villi, complete hydatidiform mole, and partial mole. Fractal dimension (FD) and lacunar dimension (LD) were measured in each case using the box counting method. FD and LD were compared in the three groups.Results: Mean FD was highest in the normal chorionic villi (1.7520), followed by partial mole (1.6696) and complete mole (1.6438). Analysis of variance (ANOVA) showed a significant difference of FD in normal villi vs. complete (p < 0.001) and partial mole (p < 0.001). There was no significant difference of FD between complete and the partial mole. The mean LD of normal, partial, and complete molar villi was 0.5067 +/- 0.6944, 0.6063 +/- 0.09670, and 0.5551 +/- 0.11277, respectively. The mean LD was significantly increased between partial mole and normal villi (ANOVA, p < 0.006). However, there was no significant difference of LD between the partial and complete mole and between complete mole and normal villi.Conclusion: The measurement of FD and LD along with the histopathology of the lesions may be helpful to distinguish molar and nonmolar villi.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 2","pages":"82-4"},"PeriodicalIF":0.0,"publicationDate":"2011-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30191377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphometric analysis of DNA ploidy and nuclear cell cycle regulators in Ewing's sarcoma/primitive neuroectodermal tumor. Ewing肉瘤/原始神经外胚层肿瘤DNA倍体和核细胞周期调控因子的形态计量学分析。

Analytical and quantitative cytology and histology Pub Date : 2011-04-01

Isidro Machado, Amparo Ruiz-Sauri, José A López-Guerrero, Antonio Llombart-Bosch

{"title":"Morphometric analysis of DNA ploidy and nuclear cell cycle regulators in Ewing's sarcoma/primitive neuroectodermal tumor.","authors":"Isidro Machado, Amparo Ruiz-Sauri, José A López-Guerrero, Antonio Llombart-Bosch","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the morphometry of DNA ploidy as a prognostic factor, compare qualitative and quantitative methods using immunohistochemical markers, and evaluate the utility of morphometry in the differential diagnosis of Ewing's sarcoma family of tumor (ESFT) subtypes and small round cell tumors (SRCTs) of bone.Study design: A total of 111 genetically confirmed ESFTs and 19 SRCTs were evaluated for DNA content and immunohistochemistry for p53, p16, and Ki-67 using morphometric analysis. Qualitative evaluation was performed using light microscopy.Results: Of the ESFT cases, 81% were aneuploid. Overall, no statistical differences were observed regarding the nuclear area or roundness between ESFT and SRCT and between ESFT subtypes. p53 and p16 revealed high concordance between qualitative and quantitative methods and a lower agreement using Ki-67. Nuclear roundness showed prognostic significance in the univariate analysis for proportional risk survival but no independent prognostically significant variable was obtained after multivariate analysis.Conclusion: DNA content, nuclear area, or roundness cannot distinguish between ESFT and other SRCT. Morphometric analysis cannot discriminate between ESFT histologic subtypes. Quantitative measurements of IHC parameters are more precise than conventional measurements, particularly in discrepant cases (--vs. +, ++ vs. +++). DNA ploidy is not a prognostic factor in ESFT.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 2","pages":"101-10"},"PeriodicalIF":0.0,"publicationDate":"2011-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30191379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of microsatellite instability in young patients with laryngeal carcinoma. 微卫星不稳定在年轻喉癌患者中的作用。

Analytical and quantitative cytology and histology Pub Date : 2011-04-01

Laura Conde, Susana Moyano, Isabel Vilaseca, Miguel Moragas, Antonio Cardesa, Alfons Nadal

引用次数: 0

Diagnosis of intraurothelial neoplasia. Interobserver variation and the value of individual histopathologic attributes. 内皮内瘤变的诊断。观察者之间的差异和个体组织病理学属性的价值。

Analytical and quantitative cytology and histology Pub Date : 2011-04-01

Björn L Isfoss, Bernard Majak, Christer Busch, Geir J Braathen

{"title":"Diagnosis of intraurothelial neoplasia. Interobserver variation and the value of individual histopathologic attributes.","authors":"Björn L Isfoss, Bernard Majak, Christer Busch, Geir J Braathen","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To determine interobserver variation in histopathologic diagnosis of carcinoma in situ (CIS) and dysplasia (collectively intraurothelial neoplasia [IUN]) of the bladder and identify histomorphologic features important for diagnosis.Study design: A total of 272 consecutive bladder tissue samples were re-evaluated blindly by two general pathologists and one uropathologist for IUN. Discrepancies were resolved jointly. Fifteen histopathologic attributes were evaluated for prediction of diagnosis. Followup revealed recurrence and progression rates for each diagnostic category.Results: Thirty-six percent of specimens contained no evaluable flat mucosa; 51% percent of specimens from papillary urothelial neoplasia (PUN) cases showed CIS. General pathologists detected 56-69% of CIS and 8-42% of dysplasia. Histopathologic features most predictive for CIS were nuclear size, variation in nuclear shape, loss of maturation, loss of polarity, and architectural disorder. None of these individually or in combination exceeded general pathologists' diagnostic accuracy. IUN was not predictive of recurrence or progress.Conclusion: Using material mostly consisting of flat mucosa gratuitously provided in PUN resection specimens, IUN carries no prognostic value. General histopathologists detect IUN poorly to moderately, and the five most discriminatory histomorphologic features are insufficient for diagnosis. Interobserver agreement for dysplasia is dismal. Absent flat mucosa in PUN resections predicts recurrence.","PeriodicalId":76995,"journal":{"name":"Analytical and quantitative cytology and histology","volume":"33 2","pages":"75-81"},"PeriodicalIF":0.0,"publicationDate":"2011-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30193988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0