Advances in Traditional Medicine最新文献

{"title":"Effects of Crataegus orientalis fruit extract on lipid accumulation in oleic acid-induced HepG2 cells","authors":"Kader Fatma Algül,&nbsp;Turgut Şekerler,&nbsp;Ali Şen,&nbsp;Gizem Gülmez,&nbsp;Azize Şener","doi":"10.1007/s13596-024-00750-5","DOIUrl":"10.1007/s13596-024-00750-5","url":null,"abstract":"<div><p>During the recent years, much great deal of research has been conducted on potential use of certain herbs and nutraceuticals to manage nonalcoholic fatty liver disease (NAFLD), specifically with those strong biological activities and antioxidant ability. In our research, the goal is to investigation the protective efficacy of the ethanol extract of <i>Crataegus orientalis</i> fruits (COE) aganist lipid accumulation in oleic acid (OA)-induced hepatocellular carcinoma cells (HepG2) at the molecular level. Firstly, antioxidant activity and lipoxygenase activity of the <i>C. orientalis</i> were determined in vitro<i>.</i> For induction of lipid accumulation in HepG2, we established an in vitro model using 1 mM OA. The <i>C. orientalis</i> was evaluated for their ability to prevent from lipid accumulation and effects on sterol regulatory element binding protein-1c (SREBP-1c), acetyl CoA carboxylase (ACC1) and fatty acid synthase (FAS) mRNA levels involved in lipid metabolism. The <i>C. orientalis</i> exhibited moderate antioxidant activity against ABTS and DPPH radicals and a significant anti-inflammatory effect against the 5-lipoxygenase enzyme. The results demonstrated that the COE (100 µg/ml) inhibited lipid accumulation (<i>p</i> &lt; 0.01) and was effective in reducing triglyceride (TG) concentration (<i>p</i> &lt; 0.001) and production of reactive oxygen species (ROS) in HepG2 cells (<i>p</i> &lt; 0.05). <i>C. orientalis</i> suppressed mRNA expression of lipid metabolism enzymes (ACC1, FAS) and transcription factor SREBP-1c (<i>p</i> &lt; 0.001, <i>p</i> &lt; 0.001 and <i>p</i> &lt; 0.01, respectively). Our findings recommend that <i>C. orientalis</i> fruits are potentially protective against lipid accumulation in hepatocytes and may have beneficial effects in the treatment of the NAFLD.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"923 - 933"},"PeriodicalIF":1.8,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140653547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precision medicine engaging nucleoside analogues: New advances to a conventional arsenal","authors":"Heba Yehia","doi":"10.1007/s13596-024-00747-0","DOIUrl":"10.1007/s13596-024-00747-0","url":null,"abstract":"<div><p>Precision medicine (PM) is increasingly being described as a paradigm shift in our understanding of medicine and as the future of clinical practice since it promises the possibility to individualize the healthcare in order to achieve optimal treatment with minimal adversity. Advances in the fields of omics, next generation sequencing (NGS), artificial intelligence (AI) and big data have strongly augmented this approach and rendered it clinically feasible instead of relying on the conventional nosology and broad symptomatic presentations. Oncology has, so far, benefited the most from this strategy as evidenced by genetic markers and biomarkers-guided pharmacotherapeutics that have found their way into guidelines and protocols. Meanwhile, nucleoside analogues, that represent a fundamental class in solid and hematologic tumors treatment, are still far from being adequately included in this trending modality due to lacking sufficient valid clinical proofs. The well-studied drugs’ paths, however, pave the way towards this goal, hoping to transfer the hype into bedside reality.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"27 - 37"},"PeriodicalIF":1.8,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140714048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vivo assessment of wound healing activity of halibut oil cream in rat model of excision wound","authors":"S. T. Shukla,&nbsp;Anu Kaushik,&nbsp;Samiullah Allahbaksh Auti,&nbsp;Dinesh Kumar,&nbsp;Supriya Kumar Das","doi":"10.1007/s13596-024-00748-z","DOIUrl":"10.1007/s13596-024-00748-z","url":null,"abstract":"<div><p>This study explores the effects of halibut oil cream on different phases of wound healing and assess its potential as a supplement for promoting wound healing in rats. The study randomly assigned five groups of male wistar rats to receive different treatments: a vehicle-control group (water-soluble cream base), a positive-control group (Povidone-iodine ointment, PI-5%), and three halibut oil cream formulation (HBOF) treatment groups with strengths of 3, 9, and 27%. An excision wound model was used to induce injury and daily as well as terminal wound healing indices such as wound area contraction, relative wound area percentage, inflammatory and proliferative phases, oxidative stress, and cytokine levels were recorded. Compared to the vehicle control group, both the PI-5% and HBOF treatment groups significantly promoted wound healing. They demonstrated reduced wound size and oxidative stress in regenerated skin tissue. Histopathological examination revealed a marked increase in collagen production, and analysis of cytokine levels indicated enhanced efficacy. These effects can be attributed to the high hydroxyproline content of halibut oil. The findings of this study suggest that topically applying halibut oil cream may serve as a useful supplement for promoting wound healing in rats. The omega-3 fatty acids and other components present such as vitamin A and D in the cream formulation demonstrated beneficial effects on wound size reduction, oxidative stress reduction, collagen production, and cytokine efficacy.</p><h3>Graphical abstract</h3> <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div><p> Graphical Abstract depicting the experimental procedure for studying wound healing. Albino Wistar rats were utilized to create a 500 mm² wound, with varying concentrations of HBOF applied topically from day (D) 1 to 15. The control group received treatment with a water-soluble cream base, while the standard group was treated with Povidone Iodine 5%. On day 16, the skin surrounding the wounds of all animals was carefully dissected, and biochemical, cytokine level, and histopathological evaluations were performed on the skin specimens. Throughout the study, measurements were taken for body weight, the inflammatory phase (D1-5), the proliferative phase (D5-15), and wound area contraction (D1-15).</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"909 - 922"},"PeriodicalIF":1.8,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140717204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An evaluation of the antioxidant capacities of Dillenia excelsa extracts and its wound healing activity in Wistar rats","authors":"Fatin Sauli,&nbsp;Hartini M. Yasin,&nbsp;Norhayati Ahmad,&nbsp;Fairuzeta Ja’afar","doi":"10.1007/s13596-024-00746-1","DOIUrl":"10.1007/s13596-024-00746-1","url":null,"abstract":"<div><p><i>Dillenia excelsa</i>, a medicinal plant found in Brunei Darussalam, has been traditionally used by locals for treating various ailments. The present study aims to investigate the presence of phytochemicals, antioxidant properties, antibacterial activities and wound healing potential of methanolic extracts of the leaves, fruits, flowers and bark of <i>Dillenia excelsa</i>. Phytochemical screening using frothing test, Salkowski test, Dragendorff’s test and ferric chloride test revealed the presence of saponins, steroids, terpenoids, alkaloids and tannins. The total phenolic content (TPC) investigated using Folin-Ciocalteu reagent and total flavonoid contents (TFC) assessed using aluminium chloride colorimetric assay yielded the highest value of 106.3 ± 2.6 mg gallic acid equivalent per gram (GAE/g) of crude leaves extract and 23.1 ± 0.5 mg quercetin equivalent per gram (QE/g) of crude leaves extract, respectively. The antioxidant capacities of the extracts were assessed and showed the leaves extract has the highest 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and ferric ion reducing antioxidant power with the value of 156.2 ± 59.3 mg GAE/g of crude extract and 2.77 ± 0.33 mg Trolox equivalent per gram (TE/g) of crude extract, respectively. Antibacterial activities against two gram-positive and two gram-negative strains using agar well diffusion method revealed only the flowers extract showed inhibition against <i>Bacillus subtilis</i> with minimum inhibitory concentration of 40 mg/mL. The in vivo <i>w</i>ound healing potential was also carried out using male Wistar rats. The high dose leaves extract (50% w/w) showed the highest reduction in wound size at day 7, 10 and day 14 compared to the control groups and other treatment groups. Based on this present study, <i>D. excelsa</i> was shown to have the potential to promote wound healing and may serve as part of the armamentarium to traditional and complementary medicine.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"891 - 908"},"PeriodicalIF":1.8,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140732694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Galla Turcica alleviates gingiva inflammation and alveolar bone resorption via regulating Th1/Th17 in a mouse model of periodontitis","authors":"Yakun Han,&nbsp;Chengcheng Yu,&nbsp;Yan Yu","doi":"10.1007/s13596-024-00745-2","DOIUrl":"10.1007/s13596-024-00745-2","url":null,"abstract":"<div><p>It establishes that inflammatory disorder of Th1 and Th17 cells promotes alveolar bone damage during periodontitis. It is proved that Galla Turcica has the function of anti-inflammation and immuno-modulation. We are going to uncover the potential effect of Galla Turcica in bone protection and T helper cell regulation during periodontitis. Experimental periodontitis animal models were chosen for the presented research. We recorded the bleeding on probing, tooth mobility, the alveolar bone resorption rate of target teeth, proportion of CD4 + T-bet + , CD4 + ror-γt + subsets in gingiva and peripheral blood, L-2, IFN-γ, IL-17, RANKL concentration in GCF and peripheral blood sera. In addition, we performed cell culture experiments to illustrate how Galla Turcica affects Th1 and Th17 cell differentiation and function. The proportion of CD4 + T-bet + , CD4 + ror-γt + subsets, and the mRNA level and concentration of IL-2, IFN-γ, IL-17, and RANKL in cultured cells or culture media were detected by real-time PCR and ELISA. We established that Galla Turcica administration not just remedies periodontal bleeding and tooth movement, but reduce alveolar bone resorption rate. Also, we find Galla Turcica administration reduced gingiva Th17 cell proportion and GCF RANKL, IL-17 concentration. In cell culture experiments, Galla Turcica decreases Th17 cell differentiation, and IL-17 transcription and secretion. Moreover, our study showed that Galla Turcica reduces STAT expression and phosphorylation, which prevent Th17 cell differentiation. The outcomes of our study highlight the functions and possible mechanisms of Galla Turcica engaged in periodontal inflammations, and reveal the considerable effect of Galla Turcica in regulating Th17 cell differentiation and their likely contribution to alveolar bone resorption during periodontitis.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"881 - 890"},"PeriodicalIF":1.8,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140364091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of aromatherapy with Damask rose on the sleep quality of mothers with premature newborns: a quasi-experimental study","authors":"Fatemeh Hashemi,&nbsp;Ghazaleh Heydarirad,&nbsp;Fatemeh Asadollah,&nbsp;Lida Nikfarid","doi":"10.1007/s13596-024-00744-3","DOIUrl":"10.1007/s13596-024-00744-3","url":null,"abstract":"<div><p>This quasi-experimental study aimed to investigate the impact of Damask rose (DR) aromatherapy on the sleep quality of mothers with preterm infants in neonatal intensive care units (NICUs). Seventy-five eligible mothers were non-randomly divided into intervention and control groups. The control group received routine care, while the intervention group received 10% DR aromatherapy 20 min before bedtime for 10 nights. Data collection included a demographic questionnaire and the Pittsburgh Sleep Quality Index (PSQI). Statistical analysis was conducted using Chi-square, Mann–Whitney, and Wilcoxon tests with a significance level of <i>p</i> &lt; 0.05 (SPSS 16). Results revealed a significant improvement in sleep quality and its subscales in the intervention group (<i>p</i> &lt; 0.05). Although both groups showed improved total sleep scores (<i>p</i> &lt; 0.05), the intervention group exhibited a substantially greater improvement (8.31 ± 1.82 to 5.62 ± 1.91) compared to the control group (9.95 ± 2.89 to 9.47 ± 3.30), as indicated by the estimated effect size. This study demonstrates that DR aromatherapy can enhance the sleep quality of mothers with preterm infants in NICUs, suggesting its potential as a supportive intervention in neonatal care settings.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"871 - 879"},"PeriodicalIF":1.8,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140381772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive potential and chemical characterization of Planaxis sulcatus (Phylum: Mollusca, Family: Planaxidae) solvent extracts","authors":"Rasha E. M. Ali,&nbsp;Khaled M. Zayed,&nbsp;Ahmed A. Hamed,&nbsp;Mohamed R. Habib,&nbsp;Hanaa A. M. Abu ElEinin,&nbsp;Mosad A. Ghareeb,&nbsp;Rasha M. Gad El-Karim","doi":"10.1007/s13596-024-00743-4","DOIUrl":"10.1007/s13596-024-00743-4","url":null,"abstract":"<div><p>The world urgently needs to discover new drugs to meet the challenges that threaten human health. Marine molluscs are known as natural sources of various bioactive compounds. In the present study, to evaluate the biological activity of the marine snail <i>Planaxis sulcatus</i>, soft tissues of the snail were extracted using acetone (Me₂CO), ethanol (EtOH) and ethyl acetate (EtOAc)<i>. </i>All extracts were tested for their total antioxidant capacity, total phenolic and DPPH free radical scavenging activity. The extracts were also tested in vitro for their antimicrobial activities. The results showed that, EtOAc extract of <i>P. sulcatus</i> had the highest antioxidant capacity and total phenolic content, followed by acetone extract. Concerning antimicrobial and antibiofilm activities of tested extracts, EtOH extract showed a good antimicrobial and antibiofilm activity against <i>Klebsiella pneumoniae, Staphylococcus aureus and Proteus vulgaris</i>. The most promising extract, EtOH extract, was processed for cytotoxicity testing and GC–MS analysis based on antimicrobial testing. Ethanol extract revealed a significant cytotoxic effect against hepatocellular carcinoma (HePG-2) and colorectal colon cancer cell lines. GC–MS analysis indicated the presence of 41 compounds in the EtOH extract with hexadecanoic acid, ethyl ester and octadecanoic acid, ethyl ester as the main ingredients consisting 16.55% and 14.56% of the total content, respectively. These data suggest that <i>P. sulcatus</i> could be a good source for many compounds with antimicrobial and anticancer potentials.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"859 - 870"},"PeriodicalIF":1.8,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142412905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosemary (Rosmarinus officinalis L.) improves biochemical outcomes in diabetes mellitus: a systematic review and meta-analysis of animal studies","authors":"Virginia Moura Oliveira,&nbsp;Letícia Rafaela Silveira,&nbsp;Kitete Tunda Bunnel,&nbsp;Caroline Pereira Domingueti,&nbsp;André Oliveira Baldoni,&nbsp;Nayara Ragi Baldoni,&nbsp;Renê Oliveira do Couto","doi":"10.1007/s13596-024-00742-5","DOIUrl":"10.1007/s13596-024-00742-5","url":null,"abstract":"<div><p>We report on the systematic review and meta-analysis concerning the efficacy <i>of R. officinalis</i> in treating diabetes mellitus (DM) in animals. This study followed the PRISMA guideline and the protocol was registered in PROSPERO (CRD42021250556). The research was duplicated in the PubMed, Scopus, ScienceDirect, Web of Science, and Virtual Health Library (VHL) databases until December 31st, 2022. No restrictions have been set for language publication. Twenty-three (23) experimental studies of type-1 diabetes mellitus (T1DM) met the eligibility criteria and were included in the qualitative analysis, whereas eighteen (18) underwent a meta-analysis. The <i>R. officinalis</i> derivatives significantly decreased fasting plasma glucose (MD: −120.84 [95% CI; −157.09, −84.59]); increased insulin release (MD; +3.73 [95% CI; +3.17, +4.29]); dwindled blood urea nitrogen (MD: −24.84 [95% CI; −34.78, −14.90]) and creatinine (MD: −0.40 [95% CI; −0.74, −0.06]) levels; and ameliorated liver function or repaired liver damage by decreasing ALT (MD: −36.42; [95% CI; −55.69, −17.14]) and AST (MD: −24.05 [95% CI; −37.84, −10.27]) enzyme levels compared to vehicle control group. Moreover, <i>R. officinalis</i> derivatives improved the lipid profile of diabetic animals by reducing LDL-c levels (MD: −11.74 [95% CI; −21.27, −2.21]). <i>R. officinalis</i> is a nutraceutical that may help in the management of T1DM and its complications. However, some gaps need to be taken into account for this evidence. Greater attention is needed for an analytical standardization of Rosemary extracts besides the demand for high-quality clinical studies dealing with the efficacy of this phytomedicine.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"1 - 26"},"PeriodicalIF":1.8,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology and experimental validation to explore the molecular mechanisms of kidney and blood refreshing recipe for the treatment of intrauterine adhesions","authors":"Renzhi Hu,&nbsp;Min Xia,&nbsp;Shuangya Weng,&nbsp;Zhi Chen,&nbsp;Zhen Wang,&nbsp;Xuemei Zou,&nbsp;Yan Zhang,&nbsp;Yiming Chen,&nbsp;Sisi Tang","doi":"10.1007/s13596-023-00741-y","DOIUrl":"10.1007/s13596-023-00741-y","url":null,"abstract":"<div><p>Kidney and blood refreshing recipe formula is usually used to effectively treat intrauterine adhesions (IUAs). However, little is known about the underlying mechanism of kidney and blood refreshing recipes. We investigated the pharmacological mechanism of Bushen Yixue recipe on IUA through pharmacological and experimental validation. By network pharmacological analysis, 183 active constituents of the kidney and blood refreshing recipe were screened out (retrieved from TCMSP and TCM-ID). According to Venn diagram analysis, there were 102 common targets of the kidney and blood refreshing recipe. PPI analysis of the above common targets showed that there were 102 nodes and 1678 edges. Subsequently, GO enrichment and KEGG pathway analyses were also performed, which resulted in 1582 biological processes, 111 molecular functions, 82 cell components, and 174 related pathways. The active component of the kidney and blood refreshing recipe was also determined by liquid mass spectrometry. Finally, a rat model of IUA was constructed and tested in vivo. After kidney and blood refreshing recipe treatment, HE staining, Masson staining and immunohistochemistry (TGF-β1, SDF-1, VEGF) were performed on the uterine tissues of rats. The results showed that fibrosis in the uterine tissue was inhibited. The kidney and blood refreshing recipe inhibits fibrosis by inhibiting the expression of genes typical of fibrosis.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"845 - 857"},"PeriodicalIF":1.8,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-023-00741-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139850655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory potential of myricetin in leukemia cells: in silico and in vitro exploration","authors":"Khushbu Nishad,&nbsp;Talambedu Usha,&nbsp;H. P. Prashanth Kumar,&nbsp;Nijalingappa Ramesh,&nbsp;Sushil Kumar Middha","doi":"10.1007/s13596-023-00740-z","DOIUrl":"10.1007/s13596-023-00740-z","url":null,"abstract":"<div><p>A conventional plant flavonoid known as myricetin is well-known for its wide range of effects, including its strong anti-inflammatory, anti-cancer, and antioxidant characteristics. In this study, we examined anti-inflammatory potential of Myricetin (MN) by looking at the expressions of COX-2, IL-6 and TNF-α in leukemia cancer cell lines (THP-1). The THP-1 was cultured in Roswell Park Memorial Institute medium supplemented with 10% foetal bovine serum and penicillin (100 U/ml) for the purposes of the in-vitro investigations. Cell viability and cytotoxicity testing were done using the MTT assay. To prepare the ligand and proteins for the in-silico studies, The structures of the targeted anti-inflammatory proteins COX-2 (PDB ID: 5KIR), TNF- (PDB ID: 1TNF), and IL-6 (PDB ID: 1ALU) were retrieved from Protein Data Bank. Furthermore, docking experiments were carried out using the AutoDock Vina software. In comparison to the reference drug cisplatin (9.46 µg/ml) the IC50 value for MN was found to be 90.161 µg/ml. The cells pretreated with MN showed a significantly lower expression of anti-inflammatory components (COX-2, TNF-α and IL6) upon measured by geometric mean fluorescence intensity as compared to the LPS-induced control cells. Additionally, MN has shown a strong interaction with COX-2, TNF-α and IL-6 in simulation studies. To conclude, MN exhibits a potent anti-inflammatory effect both in-silico and in-vitro studies. Further, in vivo investigations are needed to gain more comprehensive understanding of the molecule.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"835 - 844"},"PeriodicalIF":1.8,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139862978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}