Network pharmacology and experimental validation to explore the molecular mechanisms of kidney and blood refreshing recipe for the treatment of intrauterine adhesions

IF 1.8 Q3 PHARMACOLOGY & PHARMACY

Advances in Traditional Medicine Pub Date : 2024-02-09 DOI:10.1007/s13596-023-00741-y

Renzhi Hu, Min Xia, Shuangya Weng, Zhi Chen, Zhen Wang, Xuemei Zou, Yan Zhang, Yiming Chen, Sisi Tang

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引用次数: 0

Abstract

Kidney and blood refreshing recipe formula is usually used to effectively treat intrauterine adhesions (IUAs). However, little is known about the underlying mechanism of kidney and blood refreshing recipes. We investigated the pharmacological mechanism of Bushen Yixue recipe on IUA through pharmacological and experimental validation. By network pharmacological analysis, 183 active constituents of the kidney and blood refreshing recipe were screened out (retrieved from TCMSP and TCM-ID). According to Venn diagram analysis, there were 102 common targets of the kidney and blood refreshing recipe. PPI analysis of the above common targets showed that there were 102 nodes and 1678 edges. Subsequently, GO enrichment and KEGG pathway analyses were also performed, which resulted in 1582 biological processes, 111 molecular functions, 82 cell components, and 174 related pathways. The active component of the kidney and blood refreshing recipe was also determined by liquid mass spectrometry. Finally, a rat model of IUA was constructed and tested in vivo. After kidney and blood refreshing recipe treatment, HE staining, Masson staining and immunohistochemistry (TGF-β1, SDF-1, VEGF) were performed on the uterine tissues of rats. The results showed that fibrosis in the uterine tissue was inhibited. The kidney and blood refreshing recipe inhibits fibrosis by inhibiting the expression of genes typical of fibrosis.

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通过网络药理学和实验验证，探索治疗宫腔内粘连的补肾养血方的分子机制

补肾养血方通常用于有效治疗宫腔内粘连（IUA）。然而，人们对补肾活血方的内在机制知之甚少。我们通过药理和实验验证，研究了布神益血方治疗宫腔粘连的药理机制。通过网络药理分析，筛选出补肾养血方中的 183 种有效成分（检索自 TCMSP 和 TCM-ID）。根据维恩图分析，补肾养血方有 102 个共同靶点。对上述共同靶标的 PPI 分析表明，共有 102 个节点和 1678 条边。随后，还进行了 GO 富集和 KEGG 通路分析，得出了 1582 个生物过程、111 个分子功能、82 个细胞成分和 174 条相关通路。此外，还通过液质联用法测定了肾脏和血液清凉配方中的活性成分。最后，构建了 IUA 大鼠模型并进行了体内试验。经过补肾养血方治疗后，对大鼠子宫组织进行了 HE 染色、Masson 染色和免疫组化（TGF-β1、SDF-1、VEGF）。结果表明，子宫组织的纤维化得到了抑制。肾脏和血液清凉配方通过抑制纤维化典型基因的表达来抑制纤维化。

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来源期刊

Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-

CiteScore

4.30

自引率

0.00%

发文量

期刊介绍： Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.