Advances in Traditional Medicine最新文献

{"title":"Enhanced pain threshold with the addition of neiguan and Hegu to cervical Jiaji electroacupuncture: a pre-post study","authors":"Oanh Thi Kim Ngo,&nbsp;Thanh Tran Van Nguyen,&nbsp;Nguyen Lam Vuong,&nbsp;Dieu-Thuong Thi Trinh","doi":"10.1007/s13596-025-00823-z","DOIUrl":"10.1007/s13596-025-00823-z","url":null,"abstract":"<div><p>The analgesic effects of 100-Hz electroacupuncture (EA) are well-documented. Jiaji C1-C4 (Ex-B2 C1-C4) have been shown to increase pressure pain threshold (PPT) across V1, V2, V3, C2-C7 dermatomes, corresponding to the cranial, facial, cervical, and upper limb regions. Hegu (LI4) and Neiguan (PC6) are located on dermatomes influenced by Ex-B2 C1-C4. This study investigates the effects of 100-Hz EA at the combination of PC6, LI4, Ex-B2 C1-C4 versus Ex-B2 C1-C4 alone. A total of 120 healthy volunteers were assigned to receive either right-sided (RE) or bilateral electroacupuncture (BE). Both groups underwent 100-Hz EA at Ex-B2 C1-C4 in stage 1 and the combination of PC6, LI4, Ex-B2 C1-C4 in stage 2, with a 7-day interval between interventions. The main outcome was the post-intervention PPT at each stage, measured at 18 locations across V1, V2, V3, C2-C7 dermatomes. After stage 1, RE group showed significant PPT increases at the right V3, C2-C7, and left V2, C3, C4, C7 dermatomes, while BE group improved across all dermatomes. Following stage 2, RE group had significant PPT increases at the right V1, V2, V3, C2, C3, C6, C7 dermatomes compared to stage 1 but not on the left, whereas BE group showed improvements across all dermatomes. No significant PPT differences were found between the groups. EA at 100-Hz on PC6, LI4, Ex-B2 C1-C4 effectively increases PPT, with BE producing more widespread effects than RE. This supports its clinical for managing pain in the cranial, facial, cervical, and upper limb regions.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"801 - 812"},"PeriodicalIF":1.3,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the antiproliferative properties of Amaryllidaceae plant species and their bioactive compounds on brain tumour cell lines","authors":"Sylvester I. Omoruyi,&nbsp;Tanya N. Augustine,&nbsp;Lawrence Mabasa,&nbsp;Ahmed A. Hussein,&nbsp;Vuyo Mavumengwana","doi":"10.1007/s13596-025-00818-w","DOIUrl":"10.1007/s13596-025-00818-w","url":null,"abstract":"<div><p>Glioblastoma multiforme is considered the most aggressive type of brain tumour due to its highly invasive properties that make complete surgical resection almost impossible and treatment very challenging. The current treatment for glioblastoma involves surgery followed by radiotherapy and chemotherapy. Despite these treatment options, tumour recurrence and toxicity from the chemotherapeutic agents remain problematic, which calls for novel treatment approaches. In this study, we investigate the antiproliferative activities of three <i>Amaryllidaceae</i> plant species, <i>Crossyne flava</i>, <i>Amaryllis belladonna</i>, and <i>Boophone haemanthiodes,</i> as well as their isolated bioactive compounds on U87 and U251 glioblastoma cell lines, with H9C2 cardiac myocyte used as a normal cell line. The effect of plant extracts and compounds on cell viability and long-term survival was determined using the MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] and clonogenic assay, respectively. Additionally, the ATP levels and apoptosis-inducing potential of the plant extracts and compounds were determined using the Promega Mitochondrial ToxGlo™ and Caspase-Glo™ 3/7 assay kits, respectively. The results reveal that both plant extracts and compounds induce cytotoxicity in glioblastoma cell lines, and the extracts also inhibit the long-term survival of U87 and U251 cells. The extracts were also selective to the cancer cells when the selectivity index was calculated. Furthermore, the plant extracts and compounds inhibited ATP production in the cancer cells, while induction of apoptosis was only evident in the compound-treated cells. Overall, our findings suggest that the <i>Amaryllidaceae</i> plant family could be a rich source of botanicals and phytochemicals that might be effective against glioblastoma.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"787 - 799"},"PeriodicalIF":1.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-025-00818-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the cognitive benefits of Eleusine Indica (L.) Gaertn in alleviating scopolamine-induced dementia in wistar rats","authors":"Neelakanth M. Jeedi,&nbsp;Reenal R. Naik,&nbsp;Shrishail K. Nimbal,&nbsp;Santosh B. Patil","doi":"10.1007/s13596-025-00819-9","DOIUrl":"10.1007/s13596-025-00819-9","url":null,"abstract":"<div><p>The loss of cognitive abilities, such as reasoning, memory, and thinking, is known as dementia. The therapeutic value of medicinal plants often arises from their antioxidants. <i>Eleusine indica’s</i> antioxidant activity in the extract is attributed to its phenolic components and abundant secondary metabolites. This study aims to discover the influence of <i>Eleusine indica</i> extract at 100 and 200 mg/kg on cognitive enhancement in scopolamine induced memory impairment in Wistar rats. Hydroethanolic extract of <i>Eleusine indica</i> was made using cold maceration followed by Soxhlet instrument, and phytochemical tests were analyzed. Dementia was induced by administration of scopolamine at the dose of 3 mg/kg. The efficacy of the extract 100 and 200 mg/kg, p.o and the standard drug piracetam 400 mg/ kg p.o. in enhancing information recall in animals was assessed through a range of experimental models, including the elevated plus maze, Morris Water Maze, and light-dark model. Biomarkers such as acetylcholinesterase and dopamine in the brain were measured along with various oxidative stress markers. Histopathology of the brain cortex and hippocampus were studied. Treatment with <i>Eleusine indica</i> extract at 200 mg/kg significantly reversed the pathogenesis induced by scopolamine, leading to an increase in acetylcholinesterase and dopamine levels. It is evident that decreases in transfer latency in elevated plus maze and Step through latency in Morris Water Maze. <i>Eleusine indica</i> extract showed strong antioxidant potency. Phytoconstituents in extract possesses free radical scavenging ability and protecting the brain structure and functions.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"777 - 785"},"PeriodicalIF":1.3,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition and biological activity of ethanolic leaf extract from Tillandsia usneoides in a murine model of acute myeloid leukemia","authors":"Laura Rojas,&nbsp;Paola Lasso,&nbsp;Natalia Murillo,&nbsp;Geison M. Costa,&nbsp;Susana Fiorentino","doi":"10.1007/s13596-024-00807-5","DOIUrl":"10.1007/s13596-024-00807-5","url":null,"abstract":"<div><p>Medicinal plants constitute a valuable reservoir for discovering novel therapeutic compounds that target leukemia and various other forms of cancer. Plants of the genus <i>Tillandsia</i>, such as <i>T. recurvata</i>, have different ethnobotanical uses, including the treatment of hemorrhoids, gastritis, arthritis, ulcers, sore throats, cancer, and diabetes. Specifically, <i>T. usneoides</i> has been used by the indigenous Zenúes in the Urabá region for diabetes management. However, few studies have been published on <i>T. usneoides</i>. The aim of this work was to determine the chemical composition of <i>T. usneoides</i> extracts and evaluate their biological activity in vitro and in vivo in a murine model of acute myeloid leukemia. The chemical composition of the extracts and fractions were analyzed by chromatographic techniques revealed the presence of cycloartane-type triterpenes and methoxylated flavonoids. The in vitro cytotoxic effects on the breast cancer (4T1 and MCF-7), melanoma (B16-F10), and leukemia (K562 and DA-3/ER-GM) cell lines of the ethanolic extract and fractions were evaluated. Furthermore, <i>T. usneoides</i> extract decreased the proliferation rate of DA-3/ER-GM cells, as well as their glucose consumption, and exerted a pro-oxidant effect. Despite the in vitro cytotoxic effects exerted on the murine leukemia cell line, the ethanolic extract of <i>T. usneoides</i> did not exhibit antitumor activity in a murine model of acute myeloid leukemia, which suggests that ex vivo analysis has no direct correlation with the in vivo effect. This observation also highlights the role of the microenvironment in regulating the activity of antitumor molecules, particularly the extract studied here. Therefore, for the development of phytomedicines, as for other antitumor molecules, extensive in vitro and in vivo analyzes are warranted to demonstrate the various interactions necessary to control tumor growth.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"763 - 776"},"PeriodicalIF":1.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-024-00807-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of intestinal tight junction assembly by Coffea arabica pulp aqueous extract: mechanism of action and role of SIRT-1","authors":"Pichayapa Sukmak,&nbsp;Laongdao Thongnak,&nbsp;Wanapas Wachiradejkul,&nbsp;Jakkapong Inchai,&nbsp;Nichapa Chindaduangratn,&nbsp;Natnicha Kitti-udom,&nbsp;Thaam Limwattananon,&nbsp;Nuttakritta Choksukchalalai,&nbsp;Wilasinee Satianrapapong,&nbsp;Sunisa Hankan,&nbsp;Doungporn Amornlerdpison,&nbsp;Atcharaporn Ontawong,&nbsp;Nattaphong Akrimajirachoote,&nbsp;Chanat Aonbangkhen,&nbsp;Chatchai Muanprasat,&nbsp;Chutima S. Vaddhanaphuti,&nbsp;Pawin Pongkorpsakol","doi":"10.1007/s13596-025-00817-x","DOIUrl":"10.1007/s13596-025-00817-x","url":null,"abstract":"<div><p>Intestinal tight junction disruption is considered as one of key pathogenic factors of several diseases including inflammatory bowel diseases. At present, there is no FDA-approved drug targeting intestinal tight junction recovery. <i>Coffea arabica</i> pulp is an agricultural waste but its aqueous extract contains a number of polyphenol-rich, bioactive compounds. The main aim of this study was to elucidate the pharmacological effects of <i>Coffea arabica</i> pulp aqueous extract (CPE) on intestinal tight junction re-assembly. Transepithelial electrical resistance (TER) measurement indicated that CPE significantly enhanced TER across the intestinal epithelial-like T84 cell monolayers in a time- and dose-dependent manner with a maximal effect being observed at 1,000 µg/ml. MTT assay and nuclear staining indicated that CPE had no cytotoxic effect on T84 cells. Fluorescein isothiocyanate (FITC)-dextran permeability assay demonstrated that CPE suppressed intestinal tight junction-dependent leak pathway permeability. In addition, the effect of CPE on enhancing intestinal tight junction assembly was not affected by inhibitors of calcium/calmodulin-dependent protein kinase kinase β (CaMKKβ), AMP-activated protein kinase (AMPK), and extracellular signal-regulated kinase (ERK). Surprisingly, sirtuin-1 (SIRT-1) inhibitors abrogated CPE-induced tight junction assembly in T84 cell monolayers. Furthermore, immunostaining indicated that CPE enhanced re-distribution of occludin and zonula occludens-1 (ZO-1) to cell junction region via SIRT-1-dependent mechanism. Collectively, CPE may be useful in the treatment of diseases related to intestinal tight junction disruption.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"319 - 329"},"PeriodicalIF":1.8,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tripterygium wilfordii attenuates IgE-mediated type I allergy by regulating Th1/Th2 balance","authors":"Xueran Xiao,&nbsp;Fei Li,&nbsp;Tongyun Long,&nbsp;Feng Xu,&nbsp;Chengyan Zhou,&nbsp;Yanfen Zhang,&nbsp;Zhongcheng Liu","doi":"10.1007/s13596-024-00815-5","DOIUrl":"10.1007/s13596-024-00815-5","url":null,"abstract":"<div><p><i>Tripterygium wilfordii</i> Hook. f. (TWHF) is a traditional Chinese medicine with multiple pharmacological activities, yet its therapeutic effects on type I hypersensitivity has not been clarified. This study investigated the treatment and potential mechanisms of TWHF and its major bioactive component Triptolide (TPL) on type I allergy. The treatment mechanism was predicted by network pharmacology. <i>Tripterygium wilfordii</i> Polycoride Tablet (TWPT) and TPL were selected as therapeutic agents for type I allergy in RBL-2H3 cell and in PCA and PSA models.25 molecular targets were screened, and IL4, IL2, PIK3CG and CXCR4 that have a strong correlation with TWHF anti-type I allergy. Furthermore, TWHF may regulate type I allergy via Th1 and Th2 cell differentiation, PI3K-Akt and calcium signaling pathway. The results in <i>vitro</i> experiments showed that TWHF can dose-dependently reduce the release of β-hex and histamine induced by IgE, based on cell morphology, intracellular Ca²⁺ concentration, cell apoptosis rate and TNF-α release, TWHF was found to significantly inhibit RBL-2H3 cell degranulation, and TWPT was more effective than TPL in treating type I allergy. Results in <i>vivo</i> indicated that TWHF can dose-dependently inhibit plasma dye exudation and tissue edema in ears, backs and paws in PCA and PSA models. In addition, we also discovered that TWHF could regulate Th1/Th2 balance in PSA model in <i>vivo</i>. In conclusion, TWHF has the potential to be utilized as a medication to treat type I allergy. This work provides new perspectives on the pharmacological mechanism of TWHF.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"747 - 762"},"PeriodicalIF":1.3,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo anti-plasmodial activity of alpha-onocerin and artesunate combination against Plasmodium berghei infected mice","authors":"Jacob Hammurabi Kwansah-Obresi,&nbsp;Aliu Moomin,&nbsp;Arnold Donkor Forkuo,&nbsp;Aaron Opoku Antwi,&nbsp;John Nii Adotey Addotey,&nbsp;Paa Kofi Tawiah Adu-Gyamfi,&nbsp;Abubakar Ibn Sidik,&nbsp;Kwesi Boadu Mensah","doi":"10.1007/s13596-024-00812-8","DOIUrl":"10.1007/s13596-024-00812-8","url":null,"abstract":"<div><p>Alpha-onocerin is a triterpenoid derived from <i>Huperzia phlegmaria</i> which is used in ethnomedicine for the treatment of fever, headaches, pains and malaria. This study was conducted to evaluate the safety, antipyretic and anti-plasmodial activity of alpha-onocerin and artesunate (ART) co-administration in ICR mice for use in traditional medicine.</p><p>The anti-plasmodial effects of alpha-onocerin (10, 30, 100, 300 mg/kg) and ART (1, 2, 4, 8, 16 mg/kg) were assessed in <i>P. berghei</i>-infected mice. Alpha-onocerin /ART were administered with a fixed dose combination of their median effective doses (ED_50s) to determine the experimental ED₅₀ (Z_exp). An isobologram was developed to identify the nature of the interaction by comparing Z_exp with the theoretical ED₅₀ (Z_add).</p><p>Alpha-onocerin (300 mg/kg) showed a similar chemosuppression (93.51 ± 2.15%) to ART (2 mg/kg, i.p.) of 97.02 ± 0.27% in the 4-day suppressive test as well as in the prophylactic test with chemosuppression at 54.94% and 69.76% for alpha-onocerin (300 mg/kg) and artesunate (2 mg/kg, i.p.) respectively. All doses of alpha-onocerin significantly (<i>p</i> &lt; 0.05) reduced pyrexia in 1 h and 2 h after their administration in the baker’s yeast test. ED₅₀s for ART and alpha-onocerin were 1.33 ± 0.11 and 13.64 ± 0.22 mg/kg, respectively. The Z_add, Z_exp and interaction index for alpha-onocerin /ART co-administration were 7.49 ± 3.46, 1.61 ± 0.78 and 0.22 respectively. The Z_exp for alpha-onocerin /ART laid below the additive isobole indicating significant (<i>p</i> &lt; 0.001) synergistic activity with ART.</p><p>Alpha-onocerin showed analgesic effects, antipyretic and synergistic anti-plasmodial effects in <i>P. berghei</i>-infected mice.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"735 - 746"},"PeriodicalIF":1.3,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-024-00812-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loranthus parasiticus extract ameliorates hyperglycemia and improves insulin sensitivity in C57BL/Ksj-db/db mice","authors":"Jung Kyung Lee,&nbsp;Jae Eun Park,&nbsp;Ji Sook Han","doi":"10.1007/s13596-024-00813-7","DOIUrl":"10.1007/s13596-024-00813-7","url":null,"abstract":"<div><p><i>Loranthus parasiticus</i> Merr. (<i>L. parasiticus</i>) is a semiparasitic plant and it has antidiabetic effects. But potential application of <i>L. parasiticus</i> to improve insulin sensitivity in mice with type 2 diabetes remains unexplored.</p><p>Herein, we aimed to investigate the potential antidiabetic effects of <i>L. parasiticus</i> extract (LPE) on hyperglycemia and insulin sensitivity in C57BL/Ksj-db/db mice. C57BL/Ksj-db/db mice were divided into three groups: diabetic control, rosiglitazone, and LPE. Db/db-control group was fed a standard semi-synthetic diet (AIN-93 G), db/db-RG group was fed AIN-93 G supplemented with rosiglitazone (RG) (0.005%, w/w), and db/db-LPE group was fed AIN-93 G supplemented with LPE (0.5%, w/w) for 6 weeks. Mice supplemented with LPE exhibited significantly lower blood glucose and glycosylated hemoglobin levels than diabetic control mice. Compared with diabetic control mice, LPE-supplemented mice exhibited a significant reduction in the homeostatic index of insulin resistance. LPE supplementation stimulated the pIRS ^Tyr612 and Akt^Ser473, as well as the activation of PI3K in the skeletal muscle insulin signaling pathway. Furthermore, LPE supplementation significantly increased the pAMPK^Thr172 and ACC^Ser79 and the expression of plasma membrane GLUT4. LPE supplementation improves insulin sensitivity and alleviates hyperglycemia in diabetic mice.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"309 - 317"},"PeriodicalIF":1.8,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ellagic acid suppresses β-defensin2 antimicrobial peptide and CCL20 chemokine in psoriasis-like HaCaT human keratinocyte","authors":"Yea Ju Han,&nbsp;Hui Su Chung,&nbsp;Hyung Seo Hwang","doi":"10.1007/s13596-024-00814-6","DOIUrl":"10.1007/s13596-024-00814-6","url":null,"abstract":"<div><p>Psoriasis is an incurable skin disease with a prevalence of 2–5% worldwide. The main lesions of psoriasis are epidermal hyper-proliferation, skin barrier damage, and excessive inflammatory response. As existing treatments clearly have a lot of limitations to psoriasis patient cure, so it is needed for solutions through natural product-based alternative research. Ellagic acid, a yellow-colored plant-derived polyphenol compound existed much in <i>punica granatum L.</i>, is known to have anti-inflammatory and whitening activity but rarely been reported on psoriasis. So, we aimed to study the psoriasis control and mechanism of action at the molecular and cellular level by ellagic acid. First, the cytotoxic concentration was measured using the CCK-8 assay. As a result, no cytotoxicity was observed up to 20 μg/mL concentration, so it was applied to all subsequent experiments. Ellagic acid suppressed the mRNA expression of antimicrobial peptides (AMPs) such as β-defensin2, which are characteristically overexpressed in psoriasis lesions. In addition, it downregulated the mRNA expression level of inflammatory cytokines and chemokines such as CXC motif chemokine ligand 8 (CXCL8) and CC motif chemokine ligand 20 (CCL20). Moreover, we observed that ellagic acid significantly inhibited IκB-phosphorylation in signal pathway through Western blot. Lastly, the trans-epithelial electrical resistance (TEER) assay results also confirmed the skin barrier recovery effect due to the anti-inflammatory activity of ellagic acid. These results suggest ellagic acid has a very high possibility of being developed as a raw material could be applied to patients with sensitive skin or intractable skin diseases like psoriasis.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"723 - 733"},"PeriodicalIF":1.3,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cucurbita ficifolia regulates the secretomes of adipocytes and macrophages in co-culture, breaking meta-inflammation","authors":"F. J. Alarcon-Aguilar,&nbsp;R. W. Rosiles-Alanis,&nbsp;G. Blancas-Flores,&nbsp;M. A. Fortis-Barrera,&nbsp;J. L. Flores-Saenz,&nbsp;E. F. Alarcon-Villaseñor,&nbsp;A. Giacoman-Martínez,&nbsp;B. Mora-Ramiro,&nbsp;J. C. Almanza-Pérez","doi":"10.1007/s13596-024-00809-3","DOIUrl":"10.1007/s13596-024-00809-3","url":null,"abstract":"<div><p><i>Cucurbita ficifolia’s</i> fruit aqueous extract has been attributed with antidiabetic and anti-inflammatory properties, offering a promising alternative in addressing meta-inflammation. Meta-inflammation plays a pivotal role in the development of metabolic dysfunction, contributes to conditions such as obesity, diabetes mellitus, and metabolic syndrome. The interactions between adipocytes and macrophages within adipose tissue cause the onset of meta-inflammation, leading to disruptions in the secretomes and transcriptomes of inflammatory cytokines among other factors. We evaluated the effects of the aqueous extract from the fruit of <i>Cucurbita ficifolia</i> on the secretomes and transcriptomes of 3T3-L1 adipocytes and RAW-264.7 macrophages, by exchange of their conditioned mediums and in co-culture conditions. Adipocytes incubated with conditionate medium from macrophages treated with the extract exhibited an increase in GLUT-4 expression, and the protein release of TNFα, accompanied by a reduction in IL-1β. In contrast, macrophages incubated with conditionate medium from adipocytes treated with the extract exhibited inhibition the release of TNFα, IL-6, and IL-10, with TLR-4 expression reduction. Interestingly, under the conditions of indirect simultaneous co-culture, the release of TNFα, IL-6, and IL-1β was disrupted, increasing PPARγ expression in 3T3-L1 adipocytes. The salicin, compound reported in <i>Cucurbita ficifolia</i> extract, had a similar effect in this last model. The aqueous extract of <i>Cucurbita ficifolia</i> demonstrates the ability to suppress meta-inflammation by correcting the secretion patterns of TNFα and IL-6 in co-culture conditions. It effectively modulates the interactions between 3T3-L1 adipocytes and RAW-264.7 macrophages, leading to an improvement in the inflammatory cytokines profile.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"709 - 721"},"PeriodicalIF":1.3,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-024-00809-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}