Advances in Traditional Medicine最新文献

{"title":"Enhancement of intestinal tight junction assembly by Coffea arabica pulp aqueous extract: mechanism of action and role of SIRT-1","authors":"Pichayapa Sukmak,&nbsp;Laongdao Thongnak,&nbsp;Wanapas Wachiradejkul,&nbsp;Jakkapong Inchai,&nbsp;Nichapa Chindaduangratn,&nbsp;Natnicha Kitti-udom,&nbsp;Thaam Limwattananon,&nbsp;Nuttakritta Choksukchalalai,&nbsp;Wilasinee Satianrapapong,&nbsp;Sunisa Hankan,&nbsp;Doungporn Amornlerdpison,&nbsp;Atcharaporn Ontawong,&nbsp;Nattaphong Akrimajirachoote,&nbsp;Chanat Aonbangkhen,&nbsp;Chatchai Muanprasat,&nbsp;Chutima S. Vaddhanaphuti,&nbsp;Pawin Pongkorpsakol","doi":"10.1007/s13596-025-00817-x","DOIUrl":"10.1007/s13596-025-00817-x","url":null,"abstract":"<div><p>Intestinal tight junction disruption is considered as one of key pathogenic factors of several diseases including inflammatory bowel diseases. At present, there is no FDA-approved drug targeting intestinal tight junction recovery. <i>Coffea arabica</i> pulp is an agricultural waste but its aqueous extract contains a number of polyphenol-rich, bioactive compounds. The main aim of this study was to elucidate the pharmacological effects of <i>Coffea arabica</i> pulp aqueous extract (CPE) on intestinal tight junction re-assembly. Transepithelial electrical resistance (TER) measurement indicated that CPE significantly enhanced TER across the intestinal epithelial-like T84 cell monolayers in a time- and dose-dependent manner with a maximal effect being observed at 1,000 µg/ml. MTT assay and nuclear staining indicated that CPE had no cytotoxic effect on T84 cells. Fluorescein isothiocyanate (FITC)-dextran permeability assay demonstrated that CPE suppressed intestinal tight junction-dependent leak pathway permeability. In addition, the effect of CPE on enhancing intestinal tight junction assembly was not affected by inhibitors of calcium/calmodulin-dependent protein kinase kinase β (CaMKKβ), AMP-activated protein kinase (AMPK), and extracellular signal-regulated kinase (ERK). Surprisingly, sirtuin-1 (SIRT-1) inhibitors abrogated CPE-induced tight junction assembly in T84 cell monolayers. Furthermore, immunostaining indicated that CPE enhanced re-distribution of occludin and zonula occludens-1 (ZO-1) to cell junction region via SIRT-1-dependent mechanism. Collectively, CPE may be useful in the treatment of diseases related to intestinal tight junction disruption.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"319 - 329"},"PeriodicalIF":1.8,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loranthus parasiticus extract ameliorates hyperglycemia and improves insulin sensitivity in C57BL/Ksj-db/db mice","authors":"Jung Kyung Lee,&nbsp;Jae Eun Park,&nbsp;Ji Sook Han","doi":"10.1007/s13596-024-00813-7","DOIUrl":"10.1007/s13596-024-00813-7","url":null,"abstract":"<div><p><i>Loranthus parasiticus</i> Merr. (<i>L. parasiticus</i>) is a semiparasitic plant and it has antidiabetic effects. But potential application of <i>L. parasiticus</i> to improve insulin sensitivity in mice with type 2 diabetes remains unexplored.</p><p>Herein, we aimed to investigate the potential antidiabetic effects of <i>L. parasiticus</i> extract (LPE) on hyperglycemia and insulin sensitivity in C57BL/Ksj-db/db mice. C57BL/Ksj-db/db mice were divided into three groups: diabetic control, rosiglitazone, and LPE. Db/db-control group was fed a standard semi-synthetic diet (AIN-93 G), db/db-RG group was fed AIN-93 G supplemented with rosiglitazone (RG) (0.005%, w/w), and db/db-LPE group was fed AIN-93 G supplemented with LPE (0.5%, w/w) for 6 weeks. Mice supplemented with LPE exhibited significantly lower blood glucose and glycosylated hemoglobin levels than diabetic control mice. Compared with diabetic control mice, LPE-supplemented mice exhibited a significant reduction in the homeostatic index of insulin resistance. LPE supplementation stimulated the pIRS ^Tyr612 and Akt^Ser473, as well as the activation of PI3K in the skeletal muscle insulin signaling pathway. Furthermore, LPE supplementation significantly increased the pAMPK^Thr172 and ACC^Ser79 and the expression of plasma membrane GLUT4. LPE supplementation improves insulin sensitivity and alleviates hyperglycemia in diabetic mice.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"309 - 317"},"PeriodicalIF":1.8,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginger-derived nanovesicles inhibit breast cancer cell proliferation by induction of apoptosis","authors":"Vinayak Sharma,&nbsp;Eshu Singhal Sinha,&nbsp;Jagtar Singh","doi":"10.1007/s13596-024-00811-9","DOIUrl":"10.1007/s13596-024-00811-9","url":null,"abstract":"<div><p>Breast cancer is a prevalent and life-threatening disease affecting women globally. Despite advances in cancer treatment, there is a pressing need for effective and low-toxicity anticancer agents. Recent research has highlighted the potential of nanosized extracellular vesicles derived from edible plants in modulating cell function and facilitating biomolecule transport between cells. Mounting evidences suggests the anticancer potential of nanovesicles derived various edible plants against different types of cancer. However, the potential of nanovesicles derived from ginger in treating breast cancer remains unexplored.</p><p>In this study, we aimed to investigate the therapeutic effects of ginger derived nanovesicles (GiDNVs) on breast cancer cell lines (MDA-MB-231 and MDA-MB-468). To accomplish our goal, we isolated nanovesicles from ginger using the differential centrifugation method and characterized their size and integrity by employing DLS and FESEM. Subsequently, we evaluated the therapeutic effects of these GiDNVs on breast cancer cells. Our results demonstrated that GiDNVs inhibited cancer cell proliferation in a dosage and time-dependent way. Furthermore, flow cytometry analyses and DAPI staining demonstrated that the anti-proliferative effect of GiDNVs was attributed to the induction of apoptosis in cancer cells. Additionally, western blotting analysis showed that the apoptotic effect of GiDNVs on breast cancer cell was mainly mediated modulation by bcl-2, bax and caspase-3. This study contributes to the development of novel and effective anticancer agents with minimal toxicity. In nutstell these findings highlight the ability of GiDNVs as a novel and effective anti-cancerous agent for breast cancer treatment.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"297 - 308"},"PeriodicalIF":1.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eggplant (Solanum melongena L.) extract improves glucose uptake in LPS-induced inflammation in Caco-2/macrophages co-culture model","authors":"Atcharaporn Ontawong,&nbsp;Doungporn Amornlerdpison,&nbsp;Teerawat Rattanaphot,&nbsp;Sirinat Pengnet,&nbsp;Kullanat Kuntakhut","doi":"10.1007/s13596-024-00806-6","DOIUrl":"10.1007/s13596-024-00806-6","url":null,"abstract":"<div><p>The inflammatory bowel disease (IBD) patients have increased intestinal glucose absorption associated with hyperglycemia and diabetes mellitus. Thus, IBD treatment might minimize diabetic mellitus risk. Eggplant extract is frequently prescribed in traditional Chinese medicine and is effective against various diseases, including cancer, cardiovascular disease, and respiratory infections. It contains active compounds; chlorogenic acid (CGA) exhibits anti-inflammatory and anti-hyperglycemic effects. However, the effect of eggplant extract (EPE) on intestinal glucose uptake in IBD is unknown. Thus, this study uses LPS-induced inflammation in a co-culture model to mimic IBD. This work determined the impact of EPE glucose transport in the inflammatory co-culture model—the caco-2 cells in a transwell insert plate on LPS-induced inflammatory THP-1-derived macrophages. Caco-2 cells were treated with various doses of EPE. Inflammatory parameters and intestinal glucose transport function of Caco-2 cells were studied. <i>It was observed that</i> EPE inhibited proinflammatory cytokine gene expression and production. Furthermore, EPE inhibited intestinal glucose absorption under inflammatory conditions. These data suggested that EPE may attenuate inflammatory conditions and glucose absorption in the inflammatory co-cultures model; however, the molecular mechanisms underlying the effects of EPE must be evaluated through further investigation.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"287 - 295"},"PeriodicalIF":1.8,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the therapeutic potential of Ixora extract: a comprehensive review of mediated studies","authors":"Rabiyah Nadeem,&nbsp;Muhammad Imran,&nbsp;Zohaib Saeed,&nbsp;Muhammad Pervaiz,&nbsp;Umer Younas","doi":"10.1007/s13596-024-00797-4","DOIUrl":"10.1007/s13596-024-00797-4","url":null,"abstract":"<div><p>Traditional Herbal Medicine Systems, commonly known as Complementary and Alternative Systems of Medicine, is currently one of the most extensively researched subjects. The genus <i>Ixora</i> belongs to the family Rubiaceae, which is commonly referred to as the Madder family. It comprises 500 species and is one of the most investigated herbs today. For centuries, ethnic groups have incorporated various parts of the <i>Ixora</i> plant (flowers, leaves, stems, and roots) into Ayurvedic medicine to treat a range of ailments, including hepatic disorders, diarrhea, cancer, tumors, microbial infections, antioxidants, pain, ulcers, anti-tumor properties, and anti-inflammatory effects. Due to the extensive use of the <i>Ixora</i> genus in traditional medicine, researchers were compelled to examine the phytochemical composition of its various species. Multiple studies on the genus <i>Ixora</i>, which belongs to the Rubiaceae family, have uncovered a rich assortment of phenolic compounds. These include flavonoids, tannins, aromatic acrid oils, poly-sterols, saponins, carbohydrates, fatty acids, peptides, and terpenoids. Over the past 50 years, extensive phytochemical studies have been conducted on the genus <i>Ixora</i>, these studies have not only revealed the composition of the plant extract, but they have also helped to develop a comprehensive pharmacological profile. In doing so, these studies have provided scientific evidence supporting the ethno-medicinal uses of the plant. Every part of various <i>Ixora</i> species is said to possess beneficial properties which have added to its value as a medicinal plant. The purpose of this review is to thoroughly analyze the phytochemical and pharmacological properties of the <i>Ixora</i> genus. Along the way present an updated and categorized compilation of the scientific evidence of its medicinal properties. As a result, it aims to inspire scholars to pursue additional research on this particular genus.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"107 - 144"},"PeriodicalIF":1.8,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin and 2-DG synergistically target the glio-oncogenesis trigger IL-6 and down-regulate the stemness in glioblastoma model in-vitro","authors":"Kavita Peter,&nbsp;Puneet Gandhi,&nbsp;Santosh Kumar Kar","doi":"10.1007/s13596-024-00800-y","DOIUrl":"10.1007/s13596-024-00800-y","url":null,"abstract":"<div><p>Glioblastoma is the most lethal brain tumor; despite available multimodal therapies. This poor survival is an outcome of many contributing factors the major is resistance due to inflammation and the presence of a subset of cells called glial stem-cells (GSCs). A rationale combination of drugs which act by targeting multiple mechanisms to inhibit cancer cells and GSCs would be more potent in abrogating chemoresistance. In this study, we aimed to target cancer hallmarks using a natural polyphenol in combination with 2-deoxyglucose, a glycolytic inhibitor on the heterogeneous human U-87 Glioblastoma model. The U-87 glioblastoma cells were treated with curcumin, 2-deoxyglucose and combination of both. Cell proliferation was determined by MTT assay and combination index was analyzed. Cell cycle inhibition and apoptosis were assessed by flow cytometry. The tumorigenicity of the cells was evaluated by clonogenic and soft agar assay. Migration efficacy of the cells was assessed by wound healing assay while the inflammation trigger Il-6 was assessed by ELISA. The changes in expression of molecular marker of stemness were analyzed by immuno-cytochemistry. Curcumin enhanced the cytotoxic effect of 2-deoxyglucose in GB cancer cells. The combination of CUR (20µM) and 2-DG (4mM) showed a synergistic effect in reducing cell viability with a combination index of less than 0.9. The polyphenol induced apoptosis, arrested the cells in S and G2/M phase of the cell cycle, inhibited anchorage dependent, anchorage independent clone forming efficacy, cell migration capacity as well as stemness while 2-DG downregulated IL-6 expression potently. Our novel combination exhibited the potential to reduce glio-oncogenesis trigger Il-6 and inhibit GSCs responsible for relapse and resistance. The study suggests that our tailor-made combination using established drugs can target multiple cancer signaling molecules to yield optimal therapeutic outcome and is the right approach to address stemness attributed resistance.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"597 - 609"},"PeriodicalIF":1.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of Sphaeranthus indicus floral extract against BPS-induced testicular damage in rats occurs through downregulation of RIPK1/3-MLK-driven necroptosis and Fas-FasL-mediated apoptosis","authors":"Shabnoor Iqbal,&nbsp;Timothy Omara,&nbsp;Ivan Kahwa,&nbsp;Usman Mir Khan","doi":"10.1007/s13596-024-00803-9","DOIUrl":"10.1007/s13596-024-00803-9","url":null,"abstract":"<div><p>Bisphenol S (BPS) is one of the monomers preferred in the manufacturing of polycarbonate plastics. Unfortunately, its estrogenic and genotoxic effects are similar to those of bisphenol A. The protective effects of <i>Sphaeranthus indicus</i> floral extract (SFE) against reprotoxic effects of BPS (50 µg/kg per body weight) in rats exposed to it via drinking water was investigated. Different SFE doses (25, 50, and 100 mg/kg) were administered via oral gavage for 10 weeks. High-performance liquid chromatography (HPLC) results indicated that SFE was rich in polyphenols, with rutin and quercetin being important bioactive molecules modulating BPS-induced necroptosis and apoptosis. Biochemical analyses unveiled that rats administered BPS only exhibited considerable elevation of biomarkers of nitro-oxidative stress, necroptotic (RIPK1/RIPK3 and MLKL), and apoptotic mediators (Fas/FasL and caspase 3/caspase-8). These events caused changes in sperm characteristics (sperm motility, sperm head, and sperm viability), sperm count, and hormonal profile (thyroid stimulating hormone, luteinizing hormone, and follicle-stimulating hormone) of the rats. Histological analysis suggested that there was pronounced sloughing of Sertoli cells, reduced spermatogenic cell density, increased levels of seminiferous tubules, and disorganized morphometric parameters related to seminiferous tubules. The SFE supplementation in rats with BPS-containing water restored nitro-oxidative stress biomarkers, which led to the reduction of necroptosis and apoptosis. Reinstatement of all the biomarkers of oxidative stress, inflammation, necroptosis, and apoptosis after SFE supplementations restored the hormonal profile and normal histoarchitecture of the testes. Virtual screening elucidated that the key regulators of the necroptosis are RIPK3-rutin and RIPK1-quercetin complexes. Further studies are needed to assess its pharmacodynamics, kinetics, and effective concentration for daily use in humans.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"579 - 595"},"PeriodicalIF":1.8,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-024-00803-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annona muricata ethanolic extract protects BALB/c mice against colitis-associated colon cancer through modulation of cytokine levels and KRAS and APC expression","authors":"María del Rosario Huizar-López,&nbsp;Anne Santerre,&nbsp;Jaime Coronilla-Martínez,&nbsp;Xóchitl Rocío Ávila-Dávila,&nbsp;Pablo Cesar Ortiz-Lazareno,&nbsp;Josefina Casas-Solís","doi":"10.1007/s13596-024-00798-3","DOIUrl":"10.1007/s13596-024-00798-3","url":null,"abstract":"<div><p><i>Annona muricata</i> (L.) is used worldwide in folk medicine to treat numerous ailments. In vitro studies have reported that the phytocomponents of this plant have anti-inflammatory, antioxidant, and cytotoxic properties against multiple tumoral cells, including colon cancer cell lines. However, few studies have tested its antitumoral potential in vivo, especially against colitis-associated colon cancer (CAC), whose main risk factor is inflammation. This study assessed, for the first time, the protective properties of <i>A. muricata</i> in a murine model of CAC at the histopathological, immunological, and molecular levels. CAC was induced chemically in BALB/c mice using azoxymethane (AOM) and dextran sulfate salt (DSS). The ethanolic extract of <i>A. muricata</i> leaves was administered preventively or simultaneously with CAC induction. At the end of the experimental period, which lasted six months, colon and liver histopathology were assessed through hematoxylin and eosin staining. Circulating cytokines were evaluated by flow cytometry, and KRAS oncogene and APC tumor-suppressor gene expression by RT-qPCR. The ethanolic extract of <i>A. muricata</i> decreased circulating cytokine levels (IL-10, IL-1β, INF-γ, and IL-12) compared to the AOM-DSS group and protected colon architecture. The preventive administration of <i>A. muricata</i> also decreased the expression of oncogenic KRAS but increased the expression of APC tumor-suppressor mRNA in the colon of BALB/c mice. Administering <i>A. muricata</i> extract alone had no adverse effect on the studied parameters. Hence, our findings support the ethnobotanical use of <i>A. muricata</i>.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"553 - 565"},"PeriodicalIF":1.8,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acupuncture ameliorates liver injury induced by platinum treatment through the Notch signaling pathway","authors":"Dongdong Yu,&nbsp;Wenzhe Wang,&nbsp;Xueyuan Du,&nbsp;Yingchun Teng,&nbsp;Liqin Chao,&nbsp;Huanhuan Zhang,&nbsp;Shidong Fan,&nbsp;Xingyu Wei,&nbsp;Chen Zhang","doi":"10.1007/s13596-024-00799-2","DOIUrl":"10.1007/s13596-024-00799-2","url":null,"abstract":"<div><p>This work aims to investigate whether acupuncture can ameliorate liver injury caused by platinum-based therapy and relevant mechanism. The liver indices of patients receiving and not receiving acupuncture were analyzed based on the clinical statistics, and it was found that after acupuncture treatment, the aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin (ALB), and γ-glutamyltranspeptidase of the patients were significantly improved. Then, the animal experiments were conducted for verification. After treatment with cisplatin (DDP) modeling and acupuncture, the HE and Masson staining results of the livers of mice showed that the liver injuries were reduced, and the indexes of platelets, AST and ALT became normal after acupuncture treatment. The gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that acupuncture may ameliorates liver injury through the Notch signaling pathway. Under the effect of blocker γ-secretase inhibitor (DAPT) for the Notch signaling pathway, the expressions of inflammatory factors and the genes and proteins related to the Notch signaling pathway were all up-regulated, while the alleviation of liver injury by acupuncture was inhibited. These results suggest that acupuncture can ameliorate platinum treatment-induced liver injury through the Notch signaling pathway.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"567 - 578"},"PeriodicalIF":1.8,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-024-00799-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite profiling and wound healing potential of Gynura cusimbua in zebrafish model","authors":"Anuradha Moirangthem,&nbsp;Shandyarani Devi Laishram,&nbsp;Sonia Sougrakpam,&nbsp;Sushma Khuraijam,&nbsp;Sharmila Laishram,&nbsp;Sushil K. Chaudhary,&nbsp;Sachin Jorvekar,&nbsp;Roshan M. Borkar,&nbsp;Pulok K. Mukherjee,&nbsp;Nanaocha Sharma","doi":"10.1007/s13596-024-00788-5","DOIUrl":"10.1007/s13596-024-00788-5","url":null,"abstract":"<div><p><i>Gynura cusimbua</i> (D. Don) S. Moore is a medicinal plant traditionally used to accelerate wound healing. Scientifically, it has been reported to exhibit anti-inflammatory, anti-ulcer, hypoglycemic and anti-angiogenic activities. However, no report on wound healing activity of this plant has been studied scientifically so far. Thus, our study presents a scientific evaluation of wound healing potential of hydroalcoholic extract of <i>Gynura cusimbua</i> leaves. To identify the major phytoconstituents of hydroalcoholic extract, LCMS analysis was carried out. Protein denaturation inhibition method and membrane stabilization test was utilized to check anti- inflammatory activity of the extract. Caudal fin regeneration experiment on adult zebrafish was conducted to study wound healing potential of the extract. Developmental toxicity was studied in zebrafish embryos to rule out toxicity in the model species after systemic administration of the extract. LCMS analysis detected a total of 774 metabolites consisting of flavonoids, terpenoids, polyphenols, lipids, organic acids, phenolic acids as well as cinnamic and coumaric acid derivatives. In vitro anti-inflammatory tests revealed the extract exhibited anti-inflammatory activity in a dose dependent manner. No developmental toxicity was observed after exposure to the extract. Regeneration of caudal fin was observed in fishes exposed to the extract with significant increase in the 800 µg/mL treatment group at 5dpa and 7dpa. Neutrophil accumulation along the wounded area was observed to be highest at 24hpa. To the best of our knowledge, our study is the first report showing the wound healing potential of <i>Gynura cusimbua</i> with no developmental toxicity after exposure in the zebrafish model.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"537 - 551"},"PeriodicalIF":1.8,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}