Advances in Traditional Medicine最新文献

{"title":"Analysis of Thymus fedtschenkoi var. handelii (Ronniger), a natural provider of ursolic and oleanolic acids with significant pharmaceutical value","authors":"Serkan Yigitkan,&nbsp;Ismail Yener,&nbsp;Mehmet Akdeniz,&nbsp;Eda Cavus Kaya,&nbsp;Mustafa Abdullah Yilmaz,&nbsp;Ferhat Celik,&nbsp;Mehmet Fırat,&nbsp;Abdulselam Ertas","doi":"10.1007/s13596-025-00848-4","DOIUrl":"10.1007/s13596-025-00848-4","url":null,"abstract":"<p>The increasing interest in <i>Thymus</i> species is due to their rich phytochemical content and traditional medicinal uses. <i>Thymus fedtschenkoi</i> var. <i>handelii</i>, an endemic and rarely investigated plant, has been studied only for its essential oil. This study aimed to investigate the chemical and biological properties of the essential oil (EO) and ethanol extract (EE) of <i>T. fedtschenkoi</i> var. <i>handelii</i>. The phenolic content of the species was evaluated using LC-MS/MS and GC-MS/FID was used to analyze the chemical structure of triterpenoids, EO and aroma. Moreover, the cytotoxic and antioxidant properties as well as the suppression of tyrosinase, Hmg-CoA reductase, elastase, urease, angiotensin, and collagenase enzymes were investigated. Linalool (33.92%) and thymol (11.02%) in the EO and α-pinene (17.06%) and eucalyptol (16.20%) in the aroma were determined to be high. The species’ root and aerial component EE shown strong antioxidant activity, particularly in the ABTS, DPPH, and CUPRAC techniques. In the enzyme inhibition studies, the aerial parts and root EE showed high urease enzyme activity (80.84 ± 2.31% and 45.01 ± 0.24 at 100 µg/mL, respectively). Based on LC-MS/MS and GC-MS data, it was determined that the root of the species contained notably high concentrations of rosmarinic acid (9308.04 µg analyte/g extract), while the aerial parts contained quinic (5453.32), malic (2326.19), ursolic (145866.04), betulinic (13296.75) and oleanolic (42465.91) acids. Rich in secondary metabolites like linalool, rosmarinic acid, oleanolic acid, and ursolic acid, and exhibiting notable antioxidant and urease enzyme inhibition capacities, the species holds promise as a source of economically valuable compounds for diverse industries.</p>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"251 - 263"},"PeriodicalIF":1.3,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147338658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-clinical safety evaluation of Mangeum-tang","authors":"Sung-min Hwang,&nbsp;Sung-young Jo,&nbsp;Su-yeong Kim,&nbsp;Jun-sub Kim,&nbsp; Hyun-woo Cho,&nbsp;Jong-Hyun Nho","doi":"10.1007/s13596-025-00844-8","DOIUrl":"10.1007/s13596-025-00844-8","url":null,"abstract":"<div><p>In South Korea, the traditional use of Korean medicine is recognized by the government, with efforts being made to expand its inclusion in health insurance. However, unlike pharmaceutical drugs, traditional medicines are used in clinical practice without proper non-clinical and clinical trial validation, raising criticism and concerns. Mangeum-tang is commonly used for treating sequelae from stroke and neuralgia. The rhizome of <i>Cnidium officinale</i> MAKINO, included in Mangeum-tang, is known to reduce embryonic growth and development in rats and induce mild limb malformations, raising safety concerns. The causes of malformations are diverse, often linked to genotoxicity, prompting toxicity investigations. In vitro, Mangeum-tang did not induce genetic mutations or chromosomal aberration, and in vivo, it did not cause micronucleus formation or acute toxicity in hematopoietic cells. Therefore, Mangeum-tang does not appear to induce acute toxicity or genotoxicity. However, the studies conducted were short-term, and toxicity from repeated administration has not been confirmed. To ensure the safety of Mangeum-tang, further studies, including repeated-dose toxicity tests, carcinogenicity tests, reproductive toxicity tests, and additional research on the genotoxicity of <i>Cnidium officinale</i> MAKINO, are necessary.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"207 - 225"},"PeriodicalIF":1.3,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-025-00844-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147337431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piceatannol: a multifaceted polyphenol—from pharmacological actions to formulation development and clinical trials","authors":"Ravi Kumar Rajan,&nbsp;Bedanta Bhattacharjee,&nbsp;Hemanga Hazarika,&nbsp;Padmanath Pegu,&nbsp;Damanbhalang Rynjah,&nbsp;Maida Engels,&nbsp;Abdul Baquee Ahmed,&nbsp;Muthiah Ramanathan","doi":"10.1007/s13596-025-00845-7","DOIUrl":"10.1007/s13596-025-00845-7","url":null,"abstract":"<div><p>Piceatannol (PCT), a fascinating compound found in plants, shows great potential in drug discovery. It proves to be a superior option, closely related to its well-known counterpart, resveratrol. Thus, updating all pharmaceutical aspects of this intriguing phytomolecule is essential. While resveratrol has stolen the limelight in scientific investigations, concerns have arisen regarding its practical application due to various limitations, such as poor bioavailability and unpredictable effects at different doses. Additionally, its interactions with other drugs, lack of standardization, and limited clinical evidence have cast doubts on its widespread use. PCT, the less investigated sibling, has been gaining momentum, boasting unique advantages over resveratrol and other stilbenoids. It showcases superior bioavailability and greater metabolic stability, suggesting that the body can more effectively utilize it. Moreover, its therapeutic profile holds great promise, surpassing that of resveratrol. This comprehensive review aims to provide updated information on all aspects of PCT, exploring its therapeutic potential, chemistry, biosynthesis, available formulation, pharmacokinetics parameters, and as well as its toxicity profile. It paves the way for a deeper understanding of this remarkable compound and opens doors to further scientific exploration.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 4","pages":"837 - 884"},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145449550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin amplifies gallic acid efficacy via RGD functionalized liposomes on prostate cancer cells","authors":"Reza Mahmoudi,&nbsp;Maryam Tajali Ardakani,&nbsp;Zahra Barmoudeh,&nbsp;Shabnam Tariverdi,&nbsp;Farzad Karimpour,&nbsp;Hassan Bardania","doi":"10.1007/s13596-025-00846-6","DOIUrl":"10.1007/s13596-025-00846-6","url":null,"abstract":"<div><p>In this investigation, gallic acid (GA) and curcumin (Cur) were co-encapsulated into RGD-functionalized liposomes, denoted as GA-Cur-RFL. The synthesized liposomes were characterized, and their anticancer activity was assessed through MTT assays and annexin V/PI apoptosis kit assays conducted on PC-3 prostate cancer cells. Transmission electron microscopy (TEM) analysis revealed liposomes with a uniform morphology, approximately 100 nm in size. Dynamic light scattering (DLS) analysis further indicated a hydrodynamic size of 160.8 ± 2.3 for the liposomes. Cytotoxicity assays revealed that the cytotoxicity of GA exhibited concentration and time dependency. Moreover, the results underscored that Cur significantly enhanced the anticancer effect of GA. Additionally, the functionalization of liposomes with RGD peptide was found to increase the cytotoxicity of liposomes containing both compounds compared to free drugs and drugs encapsulated into simple liposomes. Apoptosis assays yielded compelling results, showcasing a notable improvement in late apoptosis (20% late apoptosis) for GA-Cur-RFL in comparison to non-functionalized nanoliposomes and the free form of compounds (13.6% and 12% late apoptosis, respectively). These findings conclusively demonstrate that the co-administration and delivery of Cur and GA substantially amplify their cytotoxic effects. Importantly, the enhanced apoptosis induction by GA-Cur-RFL in PC-3 cells can be attributed to the RGD modification, facilitating increased cellular absorption of GA and Cur. These insights advance our understanding of the mechanisms involved and hold promise for the development of more effective and targeted cancer therapies.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"227 - 237"},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147336617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo and in vitro evaluation of antidiarrhoeal properties of ethanol leaf and stem bark extract of Vitellaria paradoxa","authors":"Oluwakanyinsola Adeola Salawu,&nbsp;Peace Wayas Micah,&nbsp;Hadiza Mohammed Ibrahim,&nbsp;Abubakar Sadiq Mohammed,&nbsp;Ralph Elon Isaac,&nbsp;Olayide Muideen Oladepo","doi":"10.1007/s13596-025-00847-5","DOIUrl":"10.1007/s13596-025-00847-5","url":null,"abstract":"<div><p>Diarrhoea is a common cause of deaths every year. It is a major public health problem in developing countries. <i>Vitellaria paradoxa</i> is used in Africa particularly in Nigeria and Togo in empirical treatment of diarrhoea. This study evaluated the effectiveness and mode of antidiarrhoeal activity of 50% ethanol leaf and stem bark extract of <i>V. paradoxa</i> in Wistar rats and isolated rabbit ileum. In vivo antidiarrhoeal effects of <i>V. paradoxa</i> at doses of 100, 200 and 400 mg/kg body weight (bw) were evaluated using castor oil-induced diarrhoea, castor oil-induced enteropooling, and gastrointestinal motility models in rats. Isolated rabbit ileum preparation was used to assess the in vitro effect of the extract on acetylcholine- and histamine-induced contractions. The extract significantly (<i>P</i> &lt; 0.05) and dose-dependently inhibited castor oil-induced diarrhoea, intestinal transit was inhibited significantly (<i>P</i> &lt; 0.05) at doses 200 mg/kg and 400 mg/kg while the volume of the intestinal content, Na⁺ and Cl⁻ levels were not significantly increased (<i>P</i> &gt; 0.05) at doses of 100 and 200 mg/kg bw. However, significant (<i>P</i> &lt; 0.05) increase in K⁺ level was obtained at 400 mg/kg bw. Osmotic gap was not significantly (<i>P</i> &gt; 0.05) increased at all doses of extract. Acetylcholine-induced contraction was inhibited significantly (<i>P</i> &lt; 0.05) by the extract with no significant inhibition of histamine-induced contraction on the isolated rabbit ileum. These results suggest that the leaf and stem bark of <i>V. paradoxa</i> may possess antidiarrhoeal activity through antimuscarinic effects of decreased peristalsis and prolonged gastric emptying time.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"239 - 249"},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147336599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of leaf extracts of Moringa oleifera Lam. On testosterone enanthate-induced polycystic ovary syndrome (PCOS) in mice","authors":"Shakeela Khalid,&nbsp;Muhammad Arshad,&nbsp;Farzana Siddque,&nbsp;Muhammad Sajid Arshad,&nbsp;Marina Sajid,&nbsp;Waseem Khalid,&nbsp;Zaira Aziz,&nbsp;Muhammad Zubair Khalid,&nbsp;Sulaiman Ali Alharbi,&nbsp;Mohammad Javed Ansari,&nbsp;Abdellah Zinedine,&nbsp;Elena Bartkiene,&nbsp;João Miguel Rocha","doi":"10.1007/s13596-025-00843-9","DOIUrl":"10.1007/s13596-025-00843-9","url":null,"abstract":"<div><p>Polycystic ovary syndrome (PCOS) is a multifactorial endocrinological and metabolic disorder that affects women of reproductive age. It is often associated with hyperandrogenism, obesity, anovulation, infertility and insulin resistance. In this study, the imminent efficacy of MoLP (<i>Moringa oleifera</i> leaf powder) and MoLE (<i>M. oleifera</i> leaf extract) in treating PCOS in female albino mice was explored. Female albino mice were injected with testosterone enanthate [1.0 mg/100 g body weight (b.w.)] for 35-days to induce PCOS. For the treatment, mice were administered with <i>M. oleifera</i> leaf powder (250 and 500 mg/kg b.w.), <i>M. oleifera</i> leaf extract (250 and 500 mg/kg b.w.), and metformin (250 mg/kg b.w.) for 14 days. Following the intervention, body weight, blood glucose, c-peptide, testosterone, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels and stages of the estrus cycle were measured at 0, 7 and 14 days. The ovaries were examined stereologically to determine the number and diameter of follicles. Results indicated that MoLP, MoLE (500 mg/kg b.w.) and metformin (250 mg/kg b.w.) significantly decreased body weight, blood glucose, c-peptide, testosterone and LH levels while increasing FSH levels and ovary weight over time compared to PCOS-induced mice. Additionally, regulation of the estrus cycle and folliculogenesis in PCOS-induced mice was observed. Overall, the present study revealed that <i>M. oleifera</i> may have the potential as a therapeutic intervention for PCOS.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"187 - 205"},"PeriodicalIF":1.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-025-00843-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147335954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating antimicrobial, anti-biofilm, and cytotoxic effects of titanium dioxide (TiO2) nanoparticles-coated calcium hydroxide for intracanal use","authors":"Khaled Beshr,&nbsp;Hisham M. Elhalabi,&nbsp;Heba Yehia","doi":"10.1007/s13596-025-00841-x","DOIUrl":"10.1007/s13596-025-00841-x","url":null,"abstract":"<div><p>Calcium hydroxide (CH) is widely utilized in endodontic treatments for pulpal capping, sealing root canals and inter-appointment intracanal medication. For decades, it has been unrivaled due to its antibacterial properties, promoting periapical tissue mineralization, and preventing internal resorption. <i>Enterococcus faecalis</i> poses a major challenge to treatment success by resisting the chemomechanical interventions, eliciting apical periodontitis and recurring infection. Metal nanoparticles have gained increasing attention for their physicochemical properties and biological activities. Considered safe, titanium dioxide nanoparticles (TNP) have been incorporated in cosmetics and the food industry. The green synthesis of TNP bestows further advantages such as environmental safety, cost-effectiveness and easier processing. This study aimed to produce TNP using pomegranate peel waste, and use it to coat CH to formulate a novel intracanal medicament (CH-TNP), and assess its antibacterial, anti-biofilm, and biosafety properties compared to TNP and CH alone. CH-TNP’s structure, verified through spectroscopic measurements, revealed pure, smooth spherical particles averaging 182 nm in size, composed of calcium (8.66%), oxygen (70.14%) and titanium (21.9%). All samples showed antibacterial activity against Gram-positive and Gram-negative strains, as well as <i>Candida albicans</i> yeast. Confocal laser scanning microscopy demonstrated that <i>E. faecalis</i> biofilms on tooth roots were effectively eradicated by CH-TNP and CH, while TNP was less efficient. Human fibroblasts WI-38 cytotoxicity assessments using MTT assays yielded IC₅₀ values of 190.7, 162.3 and 691 μg/ml for CH-TNP, TNP and CH, respectively, with all samples eliciting minimal inflammatory response in gingival tissue.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"163 - 176"},"PeriodicalIF":1.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147339134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced anticancer potential of Punica granatum fruit extract in combination with Pheophorbide-a mediated photodynamic therapy on MCF-7 breast cancer cells","authors":"Nosipho Fakudze,&nbsp;Paromita Sarbadhikary,&nbsp;Heidi Abrahamse,&nbsp;Blassan P. George","doi":"10.1007/s13596-025-00840-y","DOIUrl":"10.1007/s13596-025-00840-y","url":null,"abstract":"<div><p>Combination therapy refers to the simultaneous administration of several therapeutic medications and treatment procedures with diverse biochemical and molecular targets and mechanisms of action. This is now used to treat a range of cancers. Furthermore, the search for less toxic natural alternatives has gained importance, which has led researchers’ interest in phytotherapy-based combination therapies. <i>Punica granatum</i> leaf, fruit, seed, and peel extracts, like many other medicinal plants, contains a high concentration of phytochemicals like tannins, anthocyanidins, phenolics, and have shown to be effective in cancer treatment. In this study, the anticancer potentials of <i>P. granatum</i> extract were evaluated in combination with a chlorophyll derivative photosensitizer, pheophorbide-a mediated photodynamic therapy (PDT) against MCF-7, a human breast cancer cell line. The effects of single and combination therapy on the viability and morphology, were examined using, adenosine triphosphate (ATP) assays, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction, and bright field and LIVE/DEAD assay respectively. The apoptotic cell death were determined by FITC-Annexin V/ Propidium Iodide assay, caspase, cytochrome-c, and Bax/Bcl-2 activity. This study demonstrated that combination treatment significantly inhibited the MCF-7 cell growth with an IC₅₀ value of 129 µg/mL compared to extract treatment alone at ~ 289 µg/mL at 24 h. Initiation of apoptosis indicated that it was mediated by the extrinsic and intrinsic pathways according to the enhanced levels of both caspase-8 and caspase-9 enzymes. Our findings suggest that a combination of phytocompound-based therapeutic modalities with different mechanisms of action has the potential to significantly improve cancer treatment efficacy when compared to single-agent therapy.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"143 - 161"},"PeriodicalIF":1.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-025-00840-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147336438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyalthia longifolia var. angustifolia thw.: a review of ethnobotany, phytochemistry, pharmacology applications and future prospects as a green anti-aging agent","authors":"Muhammad Ismaila,&nbsp;Saravanan Dharmaraj,&nbsp;Eugene Ong Boon Beng,&nbsp;Tye Gee Jun,&nbsp;Sreenivasan Sasidharan","doi":"10.1007/s13596-025-00839-5","DOIUrl":"10.1007/s13596-025-00839-5","url":null,"abstract":"<div><p>Aging involves a series of complex physiological changes that progressively impair cellular function. While chronological aging is inevitable, biological aging is influenced by modifiable factors such as oxidative stress, telomere shortening, chronic inflammation, and mitochondrial dysfunction. Recent research highlights the potential of medicinal plants in managing age-related conditions due to their rich phytochemical content. These bioactive compounds can promote cellular repair, scavenge reactive oxygen species (ROS), enhance telomerase activity, and support tissue regeneration. <i>Polyalthia longifolia</i> var. <i>angustifolia</i> (Thw.), a member of the Annonaceae family traditionally used for rejuvenation, has demonstrated significant anti-aging properties in both yeast and animal models. In vitro and in vivo studies, in particular, provide valuable insights into the anti-aging activity of this plant and its potential applications. This review explores the aging process, outlines the pharmacological profile of <i>P. longifolia</i>, and highlights its key anti-aging constituents, particularly flavonoids, tannins, phenolics, and carbohydrates, which are recognized for their well-documented antioxidant, anti-inflammatory, and cellular protective properties. Moreover, <i>P. longifolia</i> has shown promising effects against various age-associated disorders, including diabetes, hypertension, liver and kidney dysfunctions, inflammation, and oxidative damage. These benefits are largely attributed to its ability to modulate inflammatory pathways, minimize oxidative stress, and regulate abnormal cell proliferation, thereby supporting healthy aging. With its diverse pharmacological properties and abundant bioactive compounds, <i>P. longifolia</i> emerges as a promising natural agent in the field of anti-aging research. Its ethnobotanical significance, phytochemical richness, and therapeutic applications suggest strong potential for development into a sustainable, plant-based strategy to mitigate aging and related health issues.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 4","pages":"813 - 835"},"PeriodicalIF":1.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145449589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential of natural deep eutectic solvent in polyphenol extraction of Timonius flavescens (Jacq) Baker as an anti-breast cancer agent and its toxicity evaluation","authors":"Muhammad Fauzan Lubis,&nbsp;Retno Murwanti,&nbsp;Poppy Anjelisa Zaitun Hasibuan,&nbsp;Sumaiyah Sumaiyah,&nbsp;Nur Aira Juwita,&nbsp;Ana Yulyana,&nbsp;Ririn Astyka,&nbsp;Sri Yuliasmi,&nbsp;Lokot Donna Lubis,&nbsp;Wardiyah Daulay,&nbsp;Khairani Fitri","doi":"10.1007/s13596-025-00838-6","DOIUrl":"10.1007/s13596-025-00838-6","url":null,"abstract":"<div><p>Breast cancer remains a major global health challenge, necessitating the discovery of new therapeutic agents with enhanced efficacy and minimal side effects. This study explores the potential of <i>Timonius flavescens</i> (Jacq) Baker leaf extract, rich in polyphenols, as an anti-breast cancer agent. A green solvent system using Natural Deep Eutectic Solvent (NADES), composed of choline chloride and glucose, was optimized via Central Composite Design (CCD) to maximize the extraction of phenolic and flavonoid compounds. At an MoR of 2.084 and water content of 29.581%, the optimized extract yielded a superior total phenolic content (TPC) of 66.54 ± 0.586 mg GAE/g and total flavonoid content (TFC) of 28.89 ± 0.26 mg QE/g, compared to conventional ethanol extraction. In addition, the optimized extract revealed higher antioxidant activity based on DPPH assays. In vitro cytotoxicity assessments against MCF-7, T47D, and 4T1 breast cancer cell lines revealed a dose-dependent reduction in cell viability, with IC₅₀ values of 116.20 µg/mL, 258.64 µg/mL, and 235.03 µg/mL, respectively. The extract induced G0-G1 cell cycle arrest and apoptosis via p53 upregulation and cyclin D1 downregulation, as confirmed by flow cytometry and immunocytochemistry. Additionally, an acute toxicity evaluation in mice indicated the extract’s safety, showing no significant adverse effects on hematological and biochemical parameters or organ histology. These findings highlight the therapeutic potential of <i>T. flavescens</i> leaf extract as a natural anti-breast cancer agent, supporting its further investigation in preclinical and clinical studies. Additionally, this study underscores the efficacy of NADES-based extraction in enhancing bioactive compound recovery, promoting green chemistry in pharmaceutical research.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"117 - 141"},"PeriodicalIF":1.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147342570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}