Advances in Traditional Medicine最新文献

{"title":"Hepatoprotective role of Pueraria tuberosa water extract (PTWE) in CCl4-induced liver injury through different signaling pathways","authors":"Prerana Aditi,&nbsp;Vahab Ali,&nbsp;Mayank Choubey,&nbsp;Munichandra Babu Tirumalasetty,&nbsp;Harsh Pandey,&nbsp;Shivani Srivastava,&nbsp;Yamini Bhusan Tripathi","doi":"10.1007/s13596-024-00810-w","DOIUrl":"10.1007/s13596-024-00810-w","url":null,"abstract":"<div><p>Liver damage is one of the leading diseases, resulting in high morbidity. It is more relevant in the context of bad food habits, environmental pollution, and biohazards. The present study aimed to investigate the role of semi-purified water extract of <i>Pueraria tuberosa</i> on carbon tetrachloride (CCl4)-induced liver injury and also its mechanism of action regarding transcriptomic status in liver tissue about inflammation, hypoxia, and apoptosis. Liver injury was induced in Charles foster rats via intraperitoneal injection (IP) of CCl4, 0.1 mg/100gm body weight, diluted with olive oil (30%) twice a week for 20 days. PTWE was given via oral route daily simultaneously with CCl4 at the dose of 50 mg/100 g and 100 mg/100 g body weight. On 21st day all rats were sacrificed. Biochemical tests and histological studies were done. mRNA expression of bcl-2, caspase-3, bax, and GAPDH and protein expression of iNOS, BCL-2, HIF-1α, VEGF, β-Tubulin was done. Simultaneous treatment of PTWE with CCl4 decreased the level of NO, PC, SGOT, SGPT, ALP, LPO, and iNOS, HIF-1α, VEGF, bax, and caspase-3 expression. In addition, PTWE increased the SOD, Catalase, GSH level, and bcl-2 expression as well as normalized the architecture of hepatic tissue. Immunohistochemical staining showed the decreased accumulation of CD45, VEGF, α-SMA, collagen, and desmin after PTWE treatment. This study suggests that PTWE inhibits fibrosis by reducing the accumulation of α-SMA, collagen, and desmin in CCl4-induced toxicity. The mechanism of protective action is through its anti-inflammatory (iNOS, NO, CD45), anti-apoptotic (bcl-2, bax, caspase-3), anti-hypoxic (HIF-1α, VEGF), and anti-fibrotic (α-SMA, collagen, desmin) potentials.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"693 - 708"},"PeriodicalIF":1.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginger-derived nanovesicles inhibit breast cancer cell proliferation by induction of apoptosis","authors":"Vinayak Sharma,&nbsp;Eshu Singhal Sinha,&nbsp;Jagtar Singh","doi":"10.1007/s13596-024-00811-9","DOIUrl":"10.1007/s13596-024-00811-9","url":null,"abstract":"<div><p>Breast cancer is a prevalent and life-threatening disease affecting women globally. Despite advances in cancer treatment, there is a pressing need for effective and low-toxicity anticancer agents. Recent research has highlighted the potential of nanosized extracellular vesicles derived from edible plants in modulating cell function and facilitating biomolecule transport between cells. Mounting evidences suggests the anticancer potential of nanovesicles derived various edible plants against different types of cancer. However, the potential of nanovesicles derived from ginger in treating breast cancer remains unexplored.</p><p>In this study, we aimed to investigate the therapeutic effects of ginger derived nanovesicles (GiDNVs) on breast cancer cell lines (MDA-MB-231 and MDA-MB-468). To accomplish our goal, we isolated nanovesicles from ginger using the differential centrifugation method and characterized their size and integrity by employing DLS and FESEM. Subsequently, we evaluated the therapeutic effects of these GiDNVs on breast cancer cells. Our results demonstrated that GiDNVs inhibited cancer cell proliferation in a dosage and time-dependent way. Furthermore, flow cytometry analyses and DAPI staining demonstrated that the anti-proliferative effect of GiDNVs was attributed to the induction of apoptosis in cancer cells. Additionally, western blotting analysis showed that the apoptotic effect of GiDNVs on breast cancer cell was mainly mediated modulation by bcl-2, bax and caspase-3. This study contributes to the development of novel and effective anticancer agents with minimal toxicity. In nutstell these findings highlight the ability of GiDNVs as a novel and effective anti-cancerous agent for breast cancer treatment.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"297 - 308"},"PeriodicalIF":1.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annona glabra crude extract ameliorates rheumatoid arthritis in experimental animals: Radiological and histopathological investigation","authors":"Dhirendra Vishwakarma,&nbsp;Shanti Bhushan Mishra,&nbsp;Nishi Gupta,&nbsp;Anil Kumar Singh,&nbsp;Shradhanjali Singh","doi":"10.1007/s13596-024-00801-x","DOIUrl":"10.1007/s13596-024-00801-x","url":null,"abstract":"<div><p>The plant <i>Annona glabra</i> L. (Annonaceae) has a history of traditional use in treating various ailments such as inflammation, joint pain, and arthritis. Despite this, there has been limited pharmacological research on its effectiveness for rheumatoid arthritis. This study explores the anti-arthritic properties and phytochemical composition of an aqueous-ethanolic extract (50:50) prepared from <i>Annona glabra</i> leaves. The study assessed the anti-arthritic potential through in vivo testing with Complete Freund Adjuvant models at doses of 100 and 200 mg/kg. Additionally, both qualitative and quantitative phytochemical analysis were conducted to identify the various compounds present in the extract. The findings demonstrated a dose-dependent anti-arthritic response in the Complete Freund Adjuvant model, displaying enhanced protection against arthritic lesions and body weight changes. Furthermore, <i>Annona glabra</i> exhibited significant improvements in altered hematological parameters, rheumatoid factor, and mitigated radiographic and histopathological alterations. The quantitative phytochemical analysis identified phenolic compounds and flavonoids in the extract. These results support the traditional efficacy of <i>Annona glabra</i> as a promising anti-arthritic agent, potentially suitable for inclusion in herbal formulations for the treatment of rheumatoid arthritis.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"679 - 691"},"PeriodicalIF":1.3,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-adipogenic activity of oleoresin from Nigella sativa L. seeds via modulation of PPAR-γ and C/EBP-α expression in 3T3-L1 adipocytes","authors":"Isha Gupta,&nbsp;Muthukumar Serva Peddha","doi":"10.1007/s13596-024-00808-4","DOIUrl":"10.1007/s13596-024-00808-4","url":null,"abstract":"<div><p>The in vitro antiadipogenic properties of oleoresin obtained from Nigella sativa L.(black cumin) seeds was evaluated in this study using 3T3-L1 cells as model cell line. Despite containing beneficial compounds like polyphenols, thymoquinone, flavonoids, and unsaturated fatty acids known for their antioxidant properties, its role in obesity management is not fully understood. We hypothesized that black cumin seed oleoresin treatment would inhibit differentiation of 3T3-L1 preadipocytes by regulating expression of transcription factors, peroxisome proliferator-activated receptor γ (PPAR-γ) and CCAAT enhancer binding protein α (C/EBP-α). Cells were cultured and differentiated for 8 days in Dulbecco modified Eagle media (DMEM) containing 10% fetal bovine serum, both in the absence (control) and presence of 25, 50 and 100 µg/ml oleoresin. Oil red O staining after differentiation revealed a dose-dependent, substantial reduction in lipid droplet accumulation in the differentiated adipocytes treated with oleoresin than differentiated control (DC) group. Molecular analysis revealed downregulation in cellular induction of PPAR-γ, C/EBP-α and sterol regulatory element-binding protein 1 (SREBP1) transcription factors. Furthermore, mRNA expression of particular target genes of these transcription factors— adiponectin, adipocyte fatty acid binding protein (aP2), leptin and fatty acid synthase (FAS) was also dose dependently inhibited in differentiated adipocytes by oleoresin treatment. The activation of adenosine monophosphate protein kinase (AMPK) and phosphorylation of its substrate acetyl-CoA carboxylase (ACC) was detected by western blotting showing a significant increase in their phosphorylated protein levels. Collectively, these findings suggest that black cumin seed oleoresin showed anti-obesity effects by inhibiting lipid droplet formation and modulating molecular pathways involved in adipogenesis, highlighting its potential as a natural therapeutic agent for obesity and related metabolic disorders.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><img></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"665 - 677"},"PeriodicalIF":1.3,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"p-Coumaric acid modulates PGC1-α and TFAM expression to protect cardiomyocytes from doxorubicin toxicity via mitochondrial biogenesis","authors":"Kottayath Govindan Nevin,&nbsp;Sunitha Mary Chacko,&nbsp;DhanyaKrishnan Radhakrishnan,&nbsp;Sini Hariharan,&nbsp;Arunkumar Gangadharan","doi":"10.1007/s13596-024-00805-7","DOIUrl":"10.1007/s13596-024-00805-7","url":null,"abstract":"<div><p>At present, there are no alternatives for doxorubicin (Dox), which is associated with cardiotoxicity. Countering the negative effects of Dox with natural compounds is gaining interest among the scientific community. Prior studies demonstrated that <i>p</i>-Coumaric acid (<i>p</i>CA) decreased the toxicity caused by Dox in cardiomyocytes and is expected to work through multiple cellular mechanisms. This study aimed to clarify the fundamental mechanism of how <i>p</i>CA protects the heart. Cardiomyocyte cells (H9c2) were treated with Dox, <i>p</i>CA, and a combination of <i>p</i>CA and Dox. After 24 h of treatment, gene expression of COX 2, NF-κß, PGC1-α, cytb, and TFAM was measured. Swiss ADME analysis was conducted to assess the drug-likeness characteristics, Pro-Tox for toxicity properties of <i>p</i>CA, and in silico analysis was performed to evaluate the interaction of <i>p</i>CA with Keap 1 protein. The mRNA expressions of COX-2 and NF-κß (p65) were found to be upregulated in Dox-treated cells among the different treatment groups whereas, the activity was found to be greatly reduced in <i>p</i>CA groups before and during Dox treatment. mRNA levels of PGC1-α, TFAM, and Cyt b were found to be upregulated in the <i>p</i>CA pre and cotreated groups when compared to Dox-treated cells indicating the involvement of mitochondrial biogenesis. In silico analysis showed that <i>p</i>CA has druggable properties with no toxicity, while it interacted with keap 1 protein. The results demonstrate that Dox treatment may aggravate myocardial injury which is alleviated by <i>p</i>CA by modulating the PGC1α and keap1 pathways thereby boosting mitochondrial biogenesis.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"653 - 663"},"PeriodicalIF":1.3,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eggplant (Solanum melongena L.) extract improves glucose uptake in LPS-induced inflammation in Caco-2/macrophages co-culture model","authors":"Atcharaporn Ontawong,&nbsp;Doungporn Amornlerdpison,&nbsp;Teerawat Rattanaphot,&nbsp;Sirinat Pengnet,&nbsp;Kullanat Kuntakhut","doi":"10.1007/s13596-024-00806-6","DOIUrl":"10.1007/s13596-024-00806-6","url":null,"abstract":"<div><p>The inflammatory bowel disease (IBD) patients have increased intestinal glucose absorption associated with hyperglycemia and diabetes mellitus. Thus, IBD treatment might minimize diabetic mellitus risk. Eggplant extract is frequently prescribed in traditional Chinese medicine and is effective against various diseases, including cancer, cardiovascular disease, and respiratory infections. It contains active compounds; chlorogenic acid (CGA) exhibits anti-inflammatory and anti-hyperglycemic effects. However, the effect of eggplant extract (EPE) on intestinal glucose uptake in IBD is unknown. Thus, this study uses LPS-induced inflammation in a co-culture model to mimic IBD. This work determined the impact of EPE glucose transport in the inflammatory co-culture model—the caco-2 cells in a transwell insert plate on LPS-induced inflammatory THP-1-derived macrophages. Caco-2 cells were treated with various doses of EPE. Inflammatory parameters and intestinal glucose transport function of Caco-2 cells were studied. <i>It was observed that</i> EPE inhibited proinflammatory cytokine gene expression and production. Furthermore, EPE inhibited intestinal glucose absorption under inflammatory conditions. These data suggested that EPE may attenuate inflammatory conditions and glucose absorption in the inflammatory co-cultures model; however, the molecular mechanisms underlying the effects of EPE must be evaluated through further investigation.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"287 - 295"},"PeriodicalIF":1.8,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of apoptosis induction in KG1a leukemia cells by the Ginkgo biloba extract (EGb)","authors":"Farshid Babaei,&nbsp;Soroor Abdzadeh,&nbsp;Nadereh Bakhshandeh,&nbsp;Zahra Derakhshandeh,&nbsp;Mohammad Javad Dehghan-Nayeri,&nbsp;Mohammad Reza Sarafraz Ardakani,&nbsp;Esmaeil Babaei,&nbsp;Majid Mahdavi","doi":"10.1007/s13596-024-00804-8","DOIUrl":"10.1007/s13596-024-00804-8","url":null,"abstract":"<div><p><i>Ginkgo biloba</i> is a traditional medicinal herb with anti-cancer, anti-viral, anti-inflammatory, and liver protective properties. In here, we investigated several variables related to <i>Ginkgo biloba</i> extract (EGb)-induced apoptosis in human acute myeloid leukemia KG1a cells. The MTT method was used to quantify cell survival. Following Hoechst 33,258 staining, an inverted microscope to examine the appearance of apoptotic cells was used. Furthermore, to determine the ratio of necrotic to apoptotic cells, annexin V-FITC, propidium iodide (PI) double staining was performed. Using flow cytometry analysis, alterations in the cell cycle were examined. Using the appropriate reagent kits, the activities of caspase-3 was examined. Alterations in multiple apoptosis-associated gene expression were evaluated using a quantitative real-time PCR technique. The MTT results demonstrated that EGb dramatically and dose-dependently reduced the viability of KG1a cells. EGb causes KG1a cells to contract and produce more apoptotic bodies according to observations of their morphology. According to cell cycle studies, EGb arrested the cells in the S phase of cell cycle. The validity of the cell cycle data was confirmed by the decrease in cyclin A expression, and the increase in the expression of p53, p21, cyclin E, and CDK2. qRT-PCR analysis revealed that EGb induces apoptosis by reducing Survivin, raising Noxa, and causing a time-dependent increase in Bax/Bcl-2 transcript. These results suggest EGb induces cell death, potentially offering a promising treatment approach for acute myeloid leukemia.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"639 - 651"},"PeriodicalIF":1.3,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the therapeutic potential of Ixora extract: a comprehensive review of mediated studies","authors":"Rabiyah Nadeem,&nbsp;Muhammad Imran,&nbsp;Zohaib Saeed,&nbsp;Muhammad Pervaiz,&nbsp;Umer Younas","doi":"10.1007/s13596-024-00797-4","DOIUrl":"10.1007/s13596-024-00797-4","url":null,"abstract":"<div><p>Traditional Herbal Medicine Systems, commonly known as Complementary and Alternative Systems of Medicine, is currently one of the most extensively researched subjects. The genus <i>Ixora</i> belongs to the family Rubiaceae, which is commonly referred to as the Madder family. It comprises 500 species and is one of the most investigated herbs today. For centuries, ethnic groups have incorporated various parts of the <i>Ixora</i> plant (flowers, leaves, stems, and roots) into Ayurvedic medicine to treat a range of ailments, including hepatic disorders, diarrhea, cancer, tumors, microbial infections, antioxidants, pain, ulcers, anti-tumor properties, and anti-inflammatory effects. Due to the extensive use of the <i>Ixora</i> genus in traditional medicine, researchers were compelled to examine the phytochemical composition of its various species. Multiple studies on the genus <i>Ixora</i>, which belongs to the Rubiaceae family, have uncovered a rich assortment of phenolic compounds. These include flavonoids, tannins, aromatic acrid oils, poly-sterols, saponins, carbohydrates, fatty acids, peptides, and terpenoids. Over the past 50 years, extensive phytochemical studies have been conducted on the genus <i>Ixora</i>, these studies have not only revealed the composition of the plant extract, but they have also helped to develop a comprehensive pharmacological profile. In doing so, these studies have provided scientific evidence supporting the ethno-medicinal uses of the plant. Every part of various <i>Ixora</i> species is said to possess beneficial properties which have added to its value as a medicinal plant. The purpose of this review is to thoroughly analyze the phytochemical and pharmacological properties of the <i>Ixora</i> genus. Along the way present an updated and categorized compilation of the scientific evidence of its medicinal properties. As a result, it aims to inspire scholars to pursue additional research on this particular genus.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"107 - 144"},"PeriodicalIF":1.8,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethnobotanical study of woody medicinal plants used in the traditional pharmacopoeia in the three sub-division of the Mayo-Tsanaga department (Far North, Cameroon)","authors":"Nai Emmanuel,&nbsp;Tchobsala Daniel,&nbsp;Megueni Clautilde","doi":"10.1007/s13596-024-00796-5","DOIUrl":"10.1007/s13596-024-00796-5","url":null,"abstract":"<div><p>The conservation and rational management of medicinal plants require preliminary studies of ethnobotanical. It is for this reason that the present study on the woody medicinal plants of the three sub-division of Mayo-Tsanaga subjected to anarchic exploitation was undertaken. The aim of this study was to contribute to a better understanding of woody medicinal plants used in traditional pharmacopoeia, with a view to their sustainable management for conservation and use by the population. The study was carried out with herbalists, traditional practitioners, phytotherapists or any other person who uses plants for therapeutic purposes in three sub-divisions of the Mayo-Tsanaga division through the use of ethnobotanical survey forms. The results were processed using Statgraphics plus 5.0 version 11.0 and the Excel spreadsheet program. Following our series of surveys, the studies enabled us to identify 97 plant species used in the traditional medicine, belonging to 72 genera and 31 families. The most represented families are <i>Fabaceaea</i> (21 species), <i>Combretaceae</i> (9 species), <i>Anacardiaceae</i> (8 species) and <i>Malvaceae</i> (7 species). The most popular species used in traditional pharmacopoeia were <i>Boswellia dalzielii</i> (81.3%), <i>Khaya senegalensis</i> (80.3%), <i>Ximenia americana</i> (78.2%), <i>Bauhinia rufescens</i> (73.4%), <i>Tamarindus indica</i> (77.4%), <i>Haematostaphis barteri</i> (73.6%), <i>Sarcocephalus latifolius</i> (73.9%), <i>Kigelia africana</i> (71.8%), <i>Guiera senegalensis</i> (70.8%) and <i>Sclerocarya birrea</i> (69.3%).The results of this study showed that the bark was the most frequently plant part used in the preparation of medicine. The majority of traditional medicines were prepared in the form of decoction and oral route is their common mode of administration. This study is a source of information that contribute to the knowledge of medicinal plant flora with a view to sustainable management and to safeguard of the local popular know-how. It can also be used as a database for developing this valuable resource with a view of discovering new active ingredients for use in pharmacology and the pharmaceutical industry.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"621 - 637"},"PeriodicalIF":1.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The possible involvement of KATP channels in cholestatic pruritus in mice","authors":"Arya Afrooghe,&nbsp;Maryam Shayan,&nbsp;Nazgol-Sadat Haddadi,&nbsp;Asma Rashki,&nbsp;Shahabaddin Solaimanian,&nbsp;Maryam Shokrian Zeini,&nbsp;Ahmad-Reza Dehpour","doi":"10.1007/s13596-024-00802-w","DOIUrl":"10.1007/s13596-024-00802-w","url":null,"abstract":"<div><p>Cholestatic itch dramatically impairs the quality of life of affected patients; however, the therapeutic choices are limited and far from sufficient. ATP-sensitive potassium channels (K_ATP channels) contribute to transmitting signals, including itch. Here, we investigated the role of K_ATP channels in cholestatic pruritus in mice induced by bile duct ligation (BDL). K_ATP channel openers (diazoxide and minoxidil) and blockers (glibenclamide) were administered via the intraperitoneal route in BDL and sham-operated (Sham) mice. After the open-field test, the scratching behavior in response to the intradermal vehicle injection was videotaped for one hour. We investigated the probable alterations in the dermal expression of genes encoding the K_ATP channel (<i>Kcnj8</i> and <i>Kcnj11</i>) via RT-qPCR analysis. We detected increased scratching responses in cholestatic mice regarding intradermal vehicle injection, representing cholestatic pruritus. K_ATP channel openers, diazoxide (10 mg/kg, i.p.), and minoxidil (10 mg/kg, i.p.) attenuated scratching responses in the bile duct ligated mice. On the other hand, K_ATP channel blocker glibenclamide (3 mg/kg, i.p.) intensified the scratching behavior. Moreover, pre-treatment with the sub-effective dose of glibenclamide (1 mg/kg, i.p.) reversed the anti-pruritic effects of diazoxide (10 mg/kg, i.p.) and minoxidil (10 mg/kg, i.p.). The open-field test revealed that the scratching behavior was not affected by locomotor activity. Our finding of RT-qPCR analysis also showed an increase in the expression of <i>Kcnj11</i> in BDL mice. We conclude that K_ATP channels are possibly involved in cholestatic itch. Further studies are needed to elucidate the other associations between cholestasis and itch.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 3","pages":"611 - 619"},"PeriodicalIF":1.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}