Advances in Traditional Medicine最新文献

{"title":"Curcumin and 2-DG synergistically target the glio-oncogenesis trigger IL-6 and down-regulate the stemness in glioblastoma model in-vitro","authors":"Kavita Peter,&nbsp;Puneet Gandhi,&nbsp;Santosh Kumar Kar","doi":"10.1007/s13596-024-00800-y","DOIUrl":"10.1007/s13596-024-00800-y","url":null,"abstract":"<div><p>Glioblastoma is the most lethal brain tumor; despite available multimodal therapies. This poor survival is an outcome of many contributing factors the major is resistance due to inflammation and the presence of a subset of cells called glial stem-cells (GSCs). A rationale combination of drugs which act by targeting multiple mechanisms to inhibit cancer cells and GSCs would be more potent in abrogating chemoresistance. In this study, we aimed to target cancer hallmarks using a natural polyphenol in combination with 2-deoxyglucose, a glycolytic inhibitor on the heterogeneous human U-87 Glioblastoma model. The U-87 glioblastoma cells were treated with curcumin, 2-deoxyglucose and combination of both. Cell proliferation was determined by MTT assay and combination index was analyzed. Cell cycle inhibition and apoptosis were assessed by flow cytometry. The tumorigenicity of the cells was evaluated by clonogenic and soft agar assay. Migration efficacy of the cells was assessed by wound healing assay while the inflammation trigger Il-6 was assessed by ELISA. The changes in expression of molecular marker of stemness were analyzed by immuno-cytochemistry. Curcumin enhanced the cytotoxic effect of 2-deoxyglucose in GB cancer cells. The combination of CUR (20µM) and 2-DG (4mM) showed a synergistic effect in reducing cell viability with a combination index of less than 0.9. The polyphenol induced apoptosis, arrested the cells in S and G2/M phase of the cell cycle, inhibited anchorage dependent, anchorage independent clone forming efficacy, cell migration capacity as well as stemness while 2-DG downregulated IL-6 expression potently. Our novel combination exhibited the potential to reduce glio-oncogenesis trigger Il-6 and inhibit GSCs responsible for relapse and resistance. The study suggests that our tailor-made combination using established drugs can target multiple cancer signaling molecules to yield optimal therapeutic outcome and is the right approach to address stemness attributed resistance.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"597 - 609"},"PeriodicalIF":1.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of Sphaeranthus indicus floral extract against BPS-induced testicular damage in rats occurs through downregulation of RIPK1/3-MLK-driven necroptosis and Fas-FasL-mediated apoptosis","authors":"Shabnoor Iqbal,&nbsp;Timothy Omara,&nbsp;Ivan Kahwa,&nbsp;Usman Mir Khan","doi":"10.1007/s13596-024-00803-9","DOIUrl":"10.1007/s13596-024-00803-9","url":null,"abstract":"<div><p>Bisphenol S (BPS) is one of the monomers preferred in the manufacturing of polycarbonate plastics. Unfortunately, its estrogenic and genotoxic effects are similar to those of bisphenol A. The protective effects of <i>Sphaeranthus indicus</i> floral extract (SFE) against reprotoxic effects of BPS (50 µg/kg per body weight) in rats exposed to it via drinking water was investigated. Different SFE doses (25, 50, and 100 mg/kg) were administered via oral gavage for 10 weeks. High-performance liquid chromatography (HPLC) results indicated that SFE was rich in polyphenols, with rutin and quercetin being important bioactive molecules modulating BPS-induced necroptosis and apoptosis. Biochemical analyses unveiled that rats administered BPS only exhibited considerable elevation of biomarkers of nitro-oxidative stress, necroptotic (RIPK1/RIPK3 and MLKL), and apoptotic mediators (Fas/FasL and caspase 3/caspase-8). These events caused changes in sperm characteristics (sperm motility, sperm head, and sperm viability), sperm count, and hormonal profile (thyroid stimulating hormone, luteinizing hormone, and follicle-stimulating hormone) of the rats. Histological analysis suggested that there was pronounced sloughing of Sertoli cells, reduced spermatogenic cell density, increased levels of seminiferous tubules, and disorganized morphometric parameters related to seminiferous tubules. The SFE supplementation in rats with BPS-containing water restored nitro-oxidative stress biomarkers, which led to the reduction of necroptosis and apoptosis. Reinstatement of all the biomarkers of oxidative stress, inflammation, necroptosis, and apoptosis after SFE supplementations restored the hormonal profile and normal histoarchitecture of the testes. Virtual screening elucidated that the key regulators of the necroptosis are RIPK3-rutin and RIPK1-quercetin complexes. Further studies are needed to assess its pharmacodynamics, kinetics, and effective concentration for daily use in humans.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"579 - 595"},"PeriodicalIF":1.8,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-024-00803-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annona muricata ethanolic extract protects BALB/c mice against colitis-associated colon cancer through modulation of cytokine levels and KRAS and APC expression","authors":"María del Rosario Huizar-López,&nbsp;Anne Santerre,&nbsp;Jaime Coronilla-Martínez,&nbsp;Xóchitl Rocío Ávila-Dávila,&nbsp;Pablo Cesar Ortiz-Lazareno,&nbsp;Josefina Casas-Solís","doi":"10.1007/s13596-024-00798-3","DOIUrl":"10.1007/s13596-024-00798-3","url":null,"abstract":"<div><p><i>Annona muricata</i> (L.) is used worldwide in folk medicine to treat numerous ailments. In vitro studies have reported that the phytocomponents of this plant have anti-inflammatory, antioxidant, and cytotoxic properties against multiple tumoral cells, including colon cancer cell lines. However, few studies have tested its antitumoral potential in vivo, especially against colitis-associated colon cancer (CAC), whose main risk factor is inflammation. This study assessed, for the first time, the protective properties of <i>A. muricata</i> in a murine model of CAC at the histopathological, immunological, and molecular levels. CAC was induced chemically in BALB/c mice using azoxymethane (AOM) and dextran sulfate salt (DSS). The ethanolic extract of <i>A. muricata</i> leaves was administered preventively or simultaneously with CAC induction. At the end of the experimental period, which lasted six months, colon and liver histopathology were assessed through hematoxylin and eosin staining. Circulating cytokines were evaluated by flow cytometry, and KRAS oncogene and APC tumor-suppressor gene expression by RT-qPCR. The ethanolic extract of <i>A. muricata</i> decreased circulating cytokine levels (IL-10, IL-1β, INF-γ, and IL-12) compared to the AOM-DSS group and protected colon architecture. The preventive administration of <i>A. muricata</i> also decreased the expression of oncogenic KRAS but increased the expression of APC tumor-suppressor mRNA in the colon of BALB/c mice. Administering <i>A. muricata</i> extract alone had no adverse effect on the studied parameters. Hence, our findings support the ethnobotanical use of <i>A. muricata</i>.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"553 - 565"},"PeriodicalIF":1.8,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acupuncture ameliorates liver injury induced by platinum treatment through the Notch signaling pathway","authors":"Dongdong Yu,&nbsp;Wenzhe Wang,&nbsp;Xueyuan Du,&nbsp;Yingchun Teng,&nbsp;Liqin Chao,&nbsp;Huanhuan Zhang,&nbsp;Shidong Fan,&nbsp;Xingyu Wei,&nbsp;Chen Zhang","doi":"10.1007/s13596-024-00799-2","DOIUrl":"10.1007/s13596-024-00799-2","url":null,"abstract":"<div><p>This work aims to investigate whether acupuncture can ameliorate liver injury caused by platinum-based therapy and relevant mechanism. The liver indices of patients receiving and not receiving acupuncture were analyzed based on the clinical statistics, and it was found that after acupuncture treatment, the aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin (ALB), and γ-glutamyltranspeptidase of the patients were significantly improved. Then, the animal experiments were conducted for verification. After treatment with cisplatin (DDP) modeling and acupuncture, the HE and Masson staining results of the livers of mice showed that the liver injuries were reduced, and the indexes of platelets, AST and ALT became normal after acupuncture treatment. The gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that acupuncture may ameliorates liver injury through the Notch signaling pathway. Under the effect of blocker γ-secretase inhibitor (DAPT) for the Notch signaling pathway, the expressions of inflammatory factors and the genes and proteins related to the Notch signaling pathway were all up-regulated, while the alleviation of liver injury by acupuncture was inhibited. These results suggest that acupuncture can ameliorate platinum treatment-induced liver injury through the Notch signaling pathway.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"567 - 578"},"PeriodicalIF":1.8,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-024-00799-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite profiling and wound healing potential of Gynura cusimbua in zebrafish model","authors":"Anuradha Moirangthem,&nbsp;Shandyarani Devi Laishram,&nbsp;Sonia Sougrakpam,&nbsp;Sushma Khuraijam,&nbsp;Sharmila Laishram,&nbsp;Sushil K. Chaudhary,&nbsp;Sachin Jorvekar,&nbsp;Roshan M. Borkar,&nbsp;Pulok K. Mukherjee,&nbsp;Nanaocha Sharma","doi":"10.1007/s13596-024-00788-5","DOIUrl":"10.1007/s13596-024-00788-5","url":null,"abstract":"<div><p><i>Gynura cusimbua</i> (D. Don) S. Moore is a medicinal plant traditionally used to accelerate wound healing. Scientifically, it has been reported to exhibit anti-inflammatory, anti-ulcer, hypoglycemic and anti-angiogenic activities. However, no report on wound healing activity of this plant has been studied scientifically so far. Thus, our study presents a scientific evaluation of wound healing potential of hydroalcoholic extract of <i>Gynura cusimbua</i> leaves. To identify the major phytoconstituents of hydroalcoholic extract, LCMS analysis was carried out. Protein denaturation inhibition method and membrane stabilization test was utilized to check anti- inflammatory activity of the extract. Caudal fin regeneration experiment on adult zebrafish was conducted to study wound healing potential of the extract. Developmental toxicity was studied in zebrafish embryos to rule out toxicity in the model species after systemic administration of the extract. LCMS analysis detected a total of 774 metabolites consisting of flavonoids, terpenoids, polyphenols, lipids, organic acids, phenolic acids as well as cinnamic and coumaric acid derivatives. In vitro anti-inflammatory tests revealed the extract exhibited anti-inflammatory activity in a dose dependent manner. No developmental toxicity was observed after exposure to the extract. Regeneration of caudal fin was observed in fishes exposed to the extract with significant increase in the 800 µg/mL treatment group at 5dpa and 7dpa. Neutrophil accumulation along the wounded area was observed to be highest at 24hpa. To the best of our knowledge, our study is the first report showing the wound healing potential of <i>Gynura cusimbua</i> with no developmental toxicity after exposure in the zebrafish model.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"537 - 551"},"PeriodicalIF":1.8,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactivity-guided isolation and estimation of hepatoprotective marker compounds from Onosma bracteata; a species of the controversial drug ‘Gojihva’","authors":"Udaykumar G. Vegad,&nbsp;Jigna Vadalia,&nbsp;Nira Kalwani,&nbsp;Bhavinkumar Gayakvad,&nbsp;Riya A. Desai,&nbsp;Vaibhav Bhatt,&nbsp;Sanjay P. Chauhan,&nbsp;Devang J. Pandya","doi":"10.1007/s13596-024-00795-6","DOIUrl":"10.1007/s13596-024-00795-6","url":null,"abstract":"<div><p><i>Gojihva</i> or <i>Gaozaban</i>, an Ayurvedic and Unani medicinal plant, is a commercially important drug with a controversial botanical origin. <i>Onosma bracteata</i>, officially considered <i>Gojihva</i> in India, has very few scientific validations of traditional claims, and few attempts have been made to standardise the plant. The successive extracts were screened for in vitro DPPH assay and hepatoprotective activities (HepG2 cells) to evaluate the traditional claim of <i>vishaghna</i> - antitoxic. The methanol extract has the highest antioxidant capacity of 88.48 ± 0.00% at a concentration of 62.5 µg/mL. The methanol extract at a concentration of 125 µg/mL and above exhibited significant protection against paracetamol and carbon tetrachloride-induced toxicity compared to the control group and other extracts. The caffeic acid and β-sitosterol were isolated from methanol extract, with the latter being reported for the first time. A high-performance thin layer chromatography method was developed for estimating two compounds. The content of caffeic acid and β-sitosterol was found to be 19.06 mg/100 g and 40.42 mg/100 g of the plant material, respectively. Our studies have validated the hepatoprotective property and established quality parameters that are useful for standardisation of <i>O. bracteata</i> and distinguishing it from the other species of <i>Gojihva</i>.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"523 - 536"},"PeriodicalIF":1.8,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical components, antioxidant activity and antidiabetic activity (in vitro, in silico and in vivo, streptozotocin-induced diabetic rats) of extracts from Pathum Thani fragrant rice","authors":"Sompong Sansenya,&nbsp;Nopparat Buddhakala,&nbsp;Apirak Payaka,&nbsp;Orathai Sarakul,&nbsp;Moragote Buddhakala,&nbsp;Manatchanok Kongdin,&nbsp;Sasiprapha Asawawibul","doi":"10.1007/s13596-024-00794-7","DOIUrl":"10.1007/s13596-024-00794-7","url":null,"abstract":"<div><p>Rice has been reported to possess a variety of biological activities and non-colored rice demonstrates the activities less potent than colored rice. Pathum Thani fragrant rice is new strain non-colored Thai rice that become highly consumed rice. Due to less potent activity, an increase in activity of the non-colored rice is needed to perform. Cereals are rich in active compounds and exhibit a number of activities. To verify whether combination with cereals could enhance the activities of the non colored rice, therefore this study was carried out to determine chemical components, antioxidant and antidiabetic activities of ethanol extracts from Pathum Thani fragrant rice both individual and combination with cereals, compared to those from Black rice. GC-MS analysis revealed the presence of 32, 48, 63, and 66 chemicals in the ethanol extracts from Pathum Thani fragrant rice extract (PTRE), Pathum Thani fragrant rice extract combined with cereals (PTRCE), Black rice extract (BRE), and Black rice extract combined with cereals (BRCE), respectively. The docking study showed that 9-Octadecadienoic acid (Z, Z)-, one of the predominant chemicals in the extracts interacted in the active site pocket of α-glucosidase and α-amylase. PTRE exhibited antioxidant activity by DPPH scavenging activity and antidiabetic activity by inhibiting α-glucosidase activity, increasing body weight and serum insulin levels and reducing blood glucose levels in STZ diabetic rats. Interestingly, PTRCE exerted higher affective activities higher compared to PTRE. The results indicate that Pathum Thani fragrant rice can be enhanced the activities and developed as a therapeutic agent for management of free radical-related diseases and diabetes mellitus, by combination with cereals.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"509 - 522"},"PeriodicalIF":1.8,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-diarrhoea properties of ethanol extract of Citrullus colocynthis fruit pulp: in vivo and in silico studies","authors":"Asiat Na’Allah,&nbsp;Simbiat Bashir,&nbsp;Mutiu Alabi,&nbsp;Fatai A. Kareem,&nbsp;Gabriel O. Olaifa,&nbsp;Femi J. Afolabi,&nbsp;Sesan Abiodun Aransiola,&nbsp;Chandra Shekhar Seth,&nbsp;Naga Raju Maddela,&nbsp;Ram Prasad","doi":"10.1007/s13596-024-00783-w","DOIUrl":"10.1007/s13596-024-00783-w","url":null,"abstract":"<div><p>This study aimed to establish anti-diarrhoea agents, through in vivo and in silico molecular modelling from the phytoconstituents of <i>Citrullus colocynthis</i> ethanol fruit pulp extract (CCEFPE). The crystal structure of µ (mu) opioid and aquaporin 4 (AQP4) receptors were retrieved from the RCSB database, while the identified compounds from <i>C. colocynthis</i> ethanol fruit pulp extract were sequentially docked via Schrödinger suite. In CCEFPE in vivo anti-diarrhoea studies, control received distilled water (2 mL/kg, p.o.), diarrhoea was administered with castor oil or MgSO₄ (2 mL/kg, p.o.), standard drug was administered 2 mg/kg, p.o. loperamide, while CCEFPE (50, 100 and 150 mg/kg, p.o.) was administered to the treated groups. Seven hit compounds out of which 54 displayed relatively higher free binding energy for mu opioid and AQP4. Swiss ADME predicted compounds 54 to be blood–brain impermeant, have low gastrointestinal absorption, strong druglike-ness, and safety profile among the hits. In the in vivo study, the onset of diarrhoea was significantly (<i>p</i> &lt; 0.05) delayed by CCEFPE. A significant reduction (<i>p</i> &lt; 0.05) in gastrointestinal motility, enteropooling and PGE₂ in diarrhoea-induced groups was also observed after CCEFPE administration. Compound 54, even though it has drug-like properties, displayed a weak interaction with the receptor and hence can be structurally modified to an analogue of loperamide for a better ligand-receptor interaction while the in vivo studies confirmed the anti-diarrhoea activities of <i>C. colocynthis</i> ethanol fruit pulp extract.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"269 - 285"},"PeriodicalIF":1.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbal medicine: a potent booster for stem cell therapy in cardiovascular diseases","authors":"Amir Hossein Kheirkhah,&nbsp;Maria Kavianpour,&nbsp;Shima Ababzadeh","doi":"10.1007/s13596-024-00793-8","DOIUrl":"10.1007/s13596-024-00793-8","url":null,"abstract":"<div><p>The fact that cardiovascular diseases have continued to be the top cause of death across the world over the course of many years hammers home the crucial need of discovering breakthrough therapeutic approaches. Stem cell therapy has emerged as a potentially beneficial method due to the fact that it has the ability to boost functional improvement and facilitate the regeneration of tissue. This has led to the application of stem cell therapy by the medical community. On the other hand, its therapeutic efficacy has been impeded by a variety of obstacles, including insufficient engraftment and survival of transplanted cells. These difficulties have made it difficult for it to be effective. In this context, herbal medicine, which has been utilised for a very long time and has a wide variety of bioactive components, presents itself as a powerful instrument that has the potential to enhance stem cell therapy for cardiovascular diseases.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"395 - 410"},"PeriodicalIF":1.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of silver nanoparticles using Pterorhachis zenkeri: characterization and evaluation of antioxidant, anti-apoptotic, and androgenic properties in TM3 leydig cells exposed to cyclophosphamide","authors":"Patrick Brice Defo Deeh,&nbsp;Nagabhishek Sirpu Natesh,&nbsp;Karthik Alagarsamy,&nbsp;Madan Kumar Arumugam,&nbsp;Ramachandran Dasnamoorthy,&nbsp;Tharunkumar Sivaji,&nbsp;Vinita Vishwakarma","doi":"10.1007/s13596-024-00792-9","DOIUrl":"10.1007/s13596-024-00792-9","url":null,"abstract":"<div><p>We fabricated silver nanoparticles (AgNPs) using <i>Pterorhachis zenkeri</i>, characterized by Atomic Force Microscopy (AFM) and determined their antioxidant potentials in vitro. Results confirmed the fabrication of AgNPs by using <i>P. zenkeri</i> as a bioreducing agent for the first time. AgNPs possessed potent antioxidant activity <i>in vitro.</i> Furthermore, the TM3 cells were treated for 24 h with AgNPs, vitamin E and cyclophosphamide (CP) at different concentrations (25, 50, 100, 250 and 500 µg/ml). The cells morphology, apoptosis, mitochondrial membrane depolarisation (MMD), Reactive Oxygen Species (ROS), caspase 3/9, oxidative stress-related enzymes, testosterone, and the mRNA expression of steroidogenic acute regulatory protein (StAR) were measured. Results revealed that AgNPs, vitamin E and CP decreased the cell viability in a dose-dependent manner, but did not affect the TM3 cells morphology after treatment. The cytotoxicity of CP in TM3 cells was alleviated after AgNPs application. For instance, AgNPs significantly (<i>p</i> &lt; 0.001–0.05) reduced the MMD, ROS production, and caspase 3/9 activities, but increased the activities of superoxide dismutase, catalase and glutathione peroxidase in the TM3 cells. Moreover, AgNPs improved testosterone production by activating StAR machineries. These results indicate that AgNPs/<i>P. zenkeri</i> could be a potential alternative drug in the management of oxidative stress and androgen deficit associated with CP chemotherapy.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"495 - 508"},"PeriodicalIF":1.8,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}