Advances in Traditional Medicine最新文献

{"title":"Leea macrophylla root extract possess potential cytotoxicity in vitro against HepG2, MCF7 cells and in vivo in EAC xenografted model","authors":"Prafulla Kumar Sahu,&nbsp;Roja Sahu,&nbsp;Durga Prasad Mishra,&nbsp;Kota Padmaja,&nbsp;Soumya Kiran Mishra","doi":"10.1007/s13596-025-00836-8","DOIUrl":"10.1007/s13596-025-00836-8","url":null,"abstract":"<div><p>Natural products, a potent reservoir of novel anticancer compounds with fewer side effects, provide a novel treatment modality to counter challenging global cancer situations. The present study investigated the anticancer activity of the ethanolic extract of <i>Leea macrophylla</i> roots (EELM). This study employed a multivariate approach to optimize the extraction of <i>Leea macrophylla</i> tuberous roots for maximum therapeutic efficiency. Cytotoxicity assessment was performed in vitro using the MTT assay, where optimized EELM exhibited 90% and 77% cytotoxicity against HepG2 and MCF-7 cells, respectively. An Ehrlich ascites carcinoma (EAC)-induced Swiss albino mouse model was used to examine the efficacy of EELM and compared with 5-fluorouracil (5-FU). Treatment with EELM significantly reduced body weight, ascitic tumor volume, and improved the lifespan of malignant mice (<i>P</i> &lt; 0.001). After EELM, all hematological parameters returned to normal. The therapy groups had considerably less angiogenesis than the induced control group. The histopathology of significant organs showed no signs of cancer in any of the treatment groups. GC‒MS analysis of EELM revealed that its anticancer activity was attributed to the presence of 13-Octadecenoic acid, methyl ester; Methyl 10,11-octadecadienoate; Tris (tert-butyldimethylsilyloxy)arsane; and Methyl 25-methylheptacosanoate, which are found to have greater affinity towards mTOR, a key regulator of the PI3K-Akt-mTOR pathway involved in cancer progression. However, this claim needs to be validated through western blot and RT-PCR. These results establish <i>Leea macrophylla</i> as a potent anticancer agent due to its significant ability to inhibit tumors in both in vitro and in vivo cancer models.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"79 - 99"},"PeriodicalIF":1.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147341115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laportea aestuans (L.) Chew leaves extract ameliorates hyperglycaemia-mediated oxido-inflammatory stress in high-fat diet/streptozotocin-induced diabetes in rats","authors":"Shaza Ibrahim Musa,&nbsp;Abiodun A. Adeyemi,&nbsp;Abayomi M. Ajayi","doi":"10.1007/s13596-025-00833-x","DOIUrl":"10.1007/s13596-025-00833-x","url":null,"abstract":"<div><p><i>Laportea aestuans</i> leaves also known as tropical nettle weed is used in ethnomedicine for managing diabetes, ulcers and inflammation. This study evaluated the ameliorative effect on hyperglycaemia-mediated oxido-inflammatory stress in reno-hepatic tissues of high-fat diet/streptozotocin-induced diabetes in rats. Male Wistar rats fed on HFD and injected with STZ (35 mg/kg) were divided into four groups (n = 6) and treated with distilled water, EELA (200 and 400 mg/kg) and metformin (250 mg/kg). A group fed on normal diet only served as control. The treatment was administered orally for 21 days, fasting blood glucose (FBG) was measured on day 7, 14 and 21. Liver enzymes, lipid profile, inflammatory cytokines and oxidative stress parameters were assessed in serum, hepatic and renal tissues, respectively. The in vitro α-amylase and α-glucosidase inhibitory activities of EELA and its fractions were also determined. Treatment of the diabetic rats with LA significantly (<i>p</i> &lt; 0.05) reduced FBG relative to the diabetic control. Liver enzymes were reduced insignificantly, howbeit lipid profile and atherogenic index was significantly reduced. The levels of CRP, IL-6 and TNF-α in serum and liver were significantly reduced in EELA-treated rats when compared with diabetic control. Malondialdehyde was significantly reduced while GSH, catalase, SOD and GST increased in hepatic and renal tissues of EELA-treated rats. EELA and its fractions demonstrated inhibitory activity in α-amylase and α-glucosidase assay. <i>Laportea aestuans</i> leaves exhibited significant antidiabetic effects in HFD/STZ-induced diabetic rats possibly through reduction in inflammation, oxidative stress, dyslipidemia, and inhibition of carbohydrate digesting enzymes.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"47 - 61"},"PeriodicalIF":1.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147339127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and efficacy investigation of carnosine, an endogenous dipeptide against hypobaric hypoxia induced muscle protein loss","authors":"Akshita Kumar,&nbsp;Iti Garg,&nbsp;Geetha Suryakumar,&nbsp;Richa Rathor","doi":"10.1007/s13596-025-00835-9","DOIUrl":"10.1007/s13596-025-00835-9","url":null,"abstract":"<div><h3>Background</h3><p>An urgent requirement necessitates maintenance of skeletal muscle health and physical performance during high altitude stress. Here, we deal with preclinical evaluation of a novel protective approach via supplementation of carnosine, an endogenous pleiotropic dipeptide. Protective efficacy of carnosine supplementation was investigated in hypobaric hypoxia (HH) exposed rat models. Further, safety study was also performed after prolonged oral administration of carnosine in rats.</p><h3>Results</h3><p>Oral supplementation with carnosine showed robust muscle protection at the dose of 50 mg/kg via increasing cross-sectional area (CSA) of myofibers. Co-supplementation of carnosine ameliorated HH induced muscle protein loss via decreasing calpain3, glucose regulating protein–78 (GRP-78) and myostatin activity and this was linked with increased Akt activity. Additionaly, carnosine also improved muscle strength via regulating energy metabolism (PGC-1α), myogenesis (Myo-G) and enhancing creatinine phosphokinase (CPK) activity. Further, safety efficacy of carnosine was also evaluated via administering different doses of carnosine for prolong period. The results suggested progressive increase in body weight, food intake, skeletal muscle weight and that was associated with enhance grip strength and myogenesis process. Interestingly, myostatin (a negative regulator of muscle mass) was declined in carnosine supplementated rats.</p><h3>Conclusion</h3><p>Carnosine showed no toxicity even when administered for long period. Ergo, mechanistic data depicted to ameliorate HH linked muscle protein loss at lower dose via influencing multiple deleterious pathways. Taken together, the results propose that endogenous pleiotropic dipeptide is a strong candidate for managing skeletal muscle health during high altitude stress condition.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"63 - 77"},"PeriodicalIF":1.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147338202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the therapeutic potential of Murraya koenigii: a comprehensive study on the neuroprotective effects against rotenone-induced Parkinson’s disease in mice","authors":"Rabia Anjum,&nbsp;Chand Raza,&nbsp;Mehwish Faheem","doi":"10.1007/s13596-025-00837-7","DOIUrl":"10.1007/s13596-025-00837-7","url":null,"abstract":"<div><p>Parkinson’s disease (PD) is the second most common neurodegenerative disease. <i>Murraya koenigii</i> (MK), often known as curry tree, with known neuroprotective properties against dementia and the oxidative stress, malfunctioning of mitochondria and scavenging activity in rotenone mediated impairments. Rotenone induces oxidative stress in CNS leading to PD-like motor dysfunctions in rodents. This study aimed to evaluate the neuroprotective effects of MK leaves extract on motor impairments, neurochemical variables, anti-oxidative indices, and expression of selected genes in a rotenone-induced mice model of neurodegeneration. PD symptoms were induced in male Swiss albino mice by treating them with 2.5 mg/kg rotenone for three weeks. The protective impact of MK at the dose of 200 mg/kg (in rotenone-exposed mice) was assessed through antioxidant tests, histological and striatal dopamine levels, beam walk, pole climb down, spontaneous locomotion and gene expression analysis. The outcomes demonstrated that MK treatment improved striatal dopamine levels in the mouse brain and reduced motor impairments. Nuclear erythroid 2-related factor 2 (Nrf-2), superoxide dismutase (Sod-2), tyrosine hydroxylase (Th) and dopamine receptors (Drd-2) and oxidative stress were profoundly modulated after 200 mg/kg MK extract administration. These findings suggest the therapeutic potential of <i>Murraya koenigii</i> extract, possibly mediated through its antioxidant properties, highlighting its prospective clinical applications in protecting the nigral dopaminergic neurons.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"101 - 115"},"PeriodicalIF":1.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147338203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological changes induced by geraniol: a route-comparative toxicity study in rats following single and 14-day repeated doses","authors":"Asad Ullah Faiz Ghalib,&nbsp;Aimen Inamullah,&nbsp;Zehra Batool","doi":"10.1007/s13596-025-00827-9","DOIUrl":"10.1007/s13596-025-00827-9","url":null,"abstract":"<div><p>Geraniol, a widely used substance in pharmacological research, fragrance, and cosmetic industries, but it is devoid of safety profile. This study aimed to evaluate the acute toxicity of geraniol using 24 h and 14 d toxicity models via oral (p.o.), intraperitoneal (i.p.), and intranasal (i.n.) routes to establish its toxicological thresholds and potential mechanism of toxicity via in-silico approach. In 24 h acute toxicity study, geraniol administered at 2500 mg/kg (p.o.), 5000 mg/kg (p.o.), and 100 mg/kg (i.n.) doses did not cause mortality. While i.p. administration resulted in dose-dependent mortality, with an LD50 of 950 mg/kg determined for this route. The 14 d toxicity study revealed no mortality at doses up to 400 mg/kg (p.o.), 150 mg/kg (i.p.), and 50 mg/kg (i.n.), highlighting its safety at these thresholds. Behavioural, hematological, and biochemical analyses revealed significant hepatotoxic effects, particularly at higher doses administered through the p.o. and i.p. routes, accompanied by hepato-histopathological alterations. These effects may be associated with potential CYP2E1-mediated mechanisms, implicating metabolic activation as a contributor of geraniol-induced hepatoxicity. Other vital organs, including brain, lungs, heart, and kidneys, remained unaffected in both studies. This comprehensive investigation identifies the safety limits of geraniol through different routes of administration and underscores its hepatotoxic potential at elevated doses. These findings emphasize the need for dose optimization and further mechanistic studies to evaluate the relevance of CYP2E1 involvement. Geraniol demonstrates a favorable safety profile within defined limits, but its hepatotoxic effects at higher doses highlight the importance of careful monitoring in therapeutic and commercial applications.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 4","pages":"1035 - 1053"},"PeriodicalIF":1.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145449657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of inflammatory, oxidative, and apoptotic pathways by cirsimaritin in colitis: A mechanistic approach","authors":"Abdelrahim Alqudah,&nbsp;Esam Qnais,&nbsp;Omar Gammoh,&nbsp;Yousra Bseiso,&nbsp;Mohammed Wedyan,&nbsp;Mohammad Alqudah,&nbsp;Amani A. Harb,&nbsp;Alaa A. A. Aljabali,&nbsp;Anwar M. Alnakhli,&nbsp;Sireen Abdul Rahim Shilbayeh","doi":"10.1007/s13596-025-00832-y","DOIUrl":"10.1007/s13596-025-00832-y","url":null,"abstract":"<div><h3>Background</h3><p>Colitis is a chronic inflammatory condition characterized by significant tissue damage and oxidative stress. Cirsimaritin, a natural dimethoxyflavone, has potential therapeutic effects, but its efficacy in treating colitis and modulating inflammation, oxidative stress, apoptosis, and histological damage remains to be fully explored.</p><h3>Methods</h3><p>In this study, colitis was induced in experimental models to evaluate the pathophysiological changes. We assessed the capacity of total antioxidant (TAC), activities of superoxide dismutase (SOD) and catalase (CAT), levels of oxidative stress markers (Malondialdehyde [MDA] and Nitric Oxide [NO]), and the gene expression of key inflammatory and apoptotic markers (NF-κB, IL-6, TNF-α, iNOS, IL-1β, TLR4, Bax, Bcl-2, Caspase-3, and Caspase-8). The therapeutic effects of cirsimaritin were analyzed through its ability to modulate these biomarkers and histopathological damage.</p><h3>Results</h3><p>The occurrence of experimental colitis produced remarkable decreases in the activities of TAC, SOD, and CAT (<i>p</i> &lt;.05) and elevation of oxidative stress markers (levels of MDA and NO <i>p</i> &lt;.01). The expression of inflammatory and apoptotic genes was significantly increased (<i>p</i> &lt;.001 for TNF-α, IL-6, TLR4, IL-1β, iNOS, NF-κB; <i>p</i> &lt;.05 for Bax, Caspase-3, and Caspase-8). Cirsimaritin treatment was able to effectively alleviate these changes, decreasing levels of inflammatory cytokines and oxidative stress markers (<i>p</i> &lt;.01) and improving TAC and antioxidant enzyme activities (<i>p</i> &lt;.05); furthermore, cirsimaritin treatment was able to down-regulate apoptotic gene expression (<i>p</i> &lt;.05). The histopathological assessment revealed improved tissue architecture in cirsimaritin-treated groups.</p><h3>Conclusion</h3><p>Cirsimaritin ameliorates colitis by inhibiting inflammation, oxidative damage, and apoptosis in the colon, suggesting its possible therapeutic use.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 4","pages":"1019 - 1033"},"PeriodicalIF":1.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145449615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Film-forming spray loaded with Salvia rosmarinus essential oil: preparation, characterization and successful treatment of alopecia by hair restoration density","authors":"Marcila Martins de Oliveira,&nbsp;Mariana Dalmagro,&nbsp;Mariana Moraes Pinc,&nbsp;Mariana Caroline Maccari,&nbsp;Camila da Silva,&nbsp;Inara Staub Prochnau,&nbsp;Kelen Menezes Flores Rossi de Aguiar,&nbsp;Ezilda Jacomassi,&nbsp;Daniela de Cássia Faglioni Boleta-Ceranto,&nbsp;Jaqueline Hoscheid","doi":"10.1007/s13596-025-00831-z","DOIUrl":"10.1007/s13596-025-00831-z","url":null,"abstract":"<div><p>Androgenetic alopecia is the main reason for seeking dermatological treatments in men. Consequently, natural sources with potential for hair revitalization, such as <i>Salvia rosmarinus</i> Spenn. (rosemary) essential oil, are under investigation. In this study, a film-forming spray incorporating rosemary essential oil was developed to promote hair density restoration. The essential oil was characterized using gas chromatography coupled with mass spectrometry, and three formulations were prepared for topical application in spray form: a negative control formulation consisting solely of an alcoholic solution with Eudragit and propylene glycol; a formulation loaded with 1.0% rosemary essential oil; and a positive control loaded with 0.25% finasteride. Physicochemical characterization of the formulations was conducted, and hair restoration density was evaluated in volunteers over 16 weeks. The major compounds in the essential oil were α-pinene, 1,8-cineole, verbenone, and geraniol. The formulations were classified as adhesive and transparent. The incorporation of essential oil did not interfere with the drying time or the volume delivered, but it slightly increased the pH. However, the pH of the formulations remained stable over a 12-month storage period. The films showed a dense and thin structure; there was no skin permeation. Clinical results showed that the film-forming spray loaded with rosemary and the positive control increased capillary density. Therefore, the film-forming spray loaded with rosemary possesses characteristics suitable for topical application, with potential efficacy in treating alopecia by promoting hair density restoration.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 4","pages":"1007 - 1017"},"PeriodicalIF":1.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145449614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosmarinic acid rich fraction from Orthosiphon aristatus (Blume) Miq. leaves induces apoptosis by inhibiting the rapamycin signalling pathway","authors":"Siti Hasyimah Suhaimi,&nbsp;Rosnani Hasham,&nbsp;Mohamad Khairul Hafiz Idris,&nbsp;Nik Nurul Najihah Nik Mat Daud,&nbsp;Mohd Amir Asyraf Mohd Hamzah","doi":"10.1007/s13596-025-00830-0","DOIUrl":"10.1007/s13596-025-00830-0","url":null,"abstract":"<div><p>The mammalian target of the rapamycin (mTOR) pathway is critical for cell survival, metabolism, growth, and protein synthesis. Nevertheless, its hyperactivation can lead to the tumour development and progression. Consequently, inhibiting the phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) and mTOR signalling routes is an effective approach to initiate apoptosis in cancer cells. <i>Orthosiphon aristatus</i> (Blume) Miq.has been reported to exhibit anticancer properties against many cancer cell lines. Notably, the rosmarinic acid-rich fraction of <i>Orthosiphon aristatus</i> (Blume) Miq. (RA-OA) have been reported to significantly contribute to the antiproliferative activities on DU-145 cell line. In this study, the antiproliferative mechanism of RA-OA was explored via PI3K/Akt/mTOR pathway using in vitro experiments and in silico molecular docking. For in vitro study, annexin V staining and flow cytometry were utilized to determine apoptosis and cell cycle distribution, respectively. A quantitative reverse transcription-polymerase chain reaction was conducted to detect the changes in PI3K, Akt, mTOR and PTEN gene expression after treatment with RA-OA while molecular docking was performed to evaluate the binding affinity of rosmarinic acid towards the key proteins in the PI3K, Akt and mTOR pathway. Apoptosis was significantly induced in cells treated with the RA-OA, where the cell cycle progression of DU-145 cell line was arrested in the S phase. The RA-OA treatment has lowered PI3K, Akt and mTOR while enhancing PTEN gene expression. RA molecule exhibited strong binding (inhibition) affinity towards PI3K, Akt, mTOR proteins with docking energies of -9.10–7.58 kcal/mol. In conclusion, antiproliferative of RA-OA occurs by inhibiting the gene expression of PI3K/Akt/mTOR pathway and its protein phosphorylation.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 4","pages":"995 - 1005"},"PeriodicalIF":1.3,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145449647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethnomedicinal knowledge of the marginalized Kumal ethnic community on utilization of medicinal plants in Nepal","authors":"Chandra Bahadur Thapa,&nbsp;Munesh Ratna Gubhaju,&nbsp;Lal Bahadur Thapa","doi":"10.1007/s13596-025-00828-8","DOIUrl":"10.1007/s13596-025-00828-8","url":null,"abstract":"<div><p>Documentation of indigenous knowledge not only preserves invaluable cultural insights but also enriches scientific research by bridging gaps between traditional wisdom and modern scientific approaches. This study documents indigenous practices on medicinal plants of a marginalized Kumal community in Nepal. Information about the medicinal plants was collected by interview with the knowledgeable household members (60 households) using standard questionnaire and focus group discussions. Eighty-two plant species used by Kumal community were reported during the study for the treatments of 35 ailments under 12 use categories. Sixty-nine plant species were dicots, 11 were monocots and 2 species were Pteridophytes. Asteraceae as the commonly used family, herbaceous species as habit, roots-rhizomes and corms as the medicinal parts, infusion and decoction as the form of application were mostly cited by the informants. The plant species <i>Zanthoxylum armatum, Litsea cubeba, Swertia chirayita, Terminalia chebula, Crateva unilocularis, Bergenia ciliata</i> had high value of frequency of citation and relative frequency of citation. Kumals are not only knowledgeable on the diversity of medicinal plants but also, they are skillful to recognize the distribution of phytochemicals among the plant parts and effectiveness of use forms against different ailments. The respiratory trouble use category had the greatest F_IC indicating that this illness is more prevalent in the study area.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 4","pages":"983 - 993"},"PeriodicalIF":1.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145449462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA DBH-antisense RNA 1 modulates the miR-612/SOX4 axis as a CeRNA to regulate the properties of liver cancer stem cells and their resistance to lenvatinib","authors":"Guang-zhen Wu,&nbsp;Yang Liu,&nbsp;Yi-wei Ren,&nbsp;Ting-ting Fan,&nbsp;Teng Zhao,&nbsp;Zhi-ping Huang,&nbsp;Kai Lu","doi":"10.1007/s13596-025-00829-7","DOIUrl":"10.1007/s13596-025-00829-7","url":null,"abstract":"<div><p>Existing studies reported that Long non-coding RNA (lncRNA) modulates the stemness of CSCs. Here, we found that LncRNA DBH-antisense RNA 1(AS1) is upregulated in liver CSCs. Forced LncRNA DBH-AS1 promotes tumorigenesis and liver CSCs self-renewal. On the other hand, LncRNA DBH-AS1 expression knockdown prevents liver CSCs from self-renewing. Mechanistically, LncRNA DBH-AS1 mediated regulation of SRY-box transcription factor 4 (SOX4), acting as a ceRNA to sponge miR-612 in liver CSCs. LncRNA DBH-AS1 enhanced liver CSCs self-renew and tumorigenesis via upregulating SOX4. Furthermore, liver cells with LncRNA DBH-AS1 knockdown are susceptible to apoptosis caused by Lenvatinib. Furthermore, the sensitivity of HCC cells with LncRNA DBH-AS1 knockdown to lenvatinib-induced cell death could be reversed by adding SOX4. In conclusion, experimental evidence revealed that METTL3-dependent m⁶A methylation was the mechanism mediating the elevated lncRNADBH-AS1 in HCC. This study demonstrated the critical role that LncRNA DBH-AS1 plays in the carcinogenesis and self-renewal of liver CSCs, which renders it the perfect target for HCC therapy.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 4","pages":"971 - 981"},"PeriodicalIF":1.3,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145449555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}