Advances in Traditional Medicine最新文献

{"title":"Therapeutic potential of freeze-dried Kaempferia galanga herbal formulation in alleviating hyperglycemia in diabetic mice: an in-vivo and in-silico investigation","authors":"Flavius Phrangsngi Nonglang,&nbsp;Revelbornstar Snaitang,&nbsp;Dhritiman Roy,&nbsp;Shankupar Rynjah,&nbsp;Surya Bhan","doi":"10.1007/s13596-024-00784-9","DOIUrl":"10.1007/s13596-024-00784-9","url":null,"abstract":"<div><p>A never-ending search is being made for a natural remedy to cure diabetes, which is one of the biggest worldwide health concerns. <i>Kaempferia galanga</i> (<i>K. galanga</i>), an important medicinal herb, is reported to have various pharmacological effects. This study aims to conduct in-depth research on the antihyperglycemic activity of <i>K galanga</i> in streptozotocin (STZ)-induced diabetic mice and the mechanism by which it elicits its response. For 21 days, various dosages of freeze-dried <i>K. galanga</i> ethanolic extract (KGE) therapy were given intraperitoneally in STZ-induced diabetic mice. KGE at 350 mg/kg b.w. was selected as the ideal dose. Acute toxicology studies have shown that KGE is non-toxic. Protein and gene expression studies targeting various metabolic pathways show that in the diabetic mice treated with KGE, there was stimulation of AKT, inhibition of glycogen synthase kinase-3, stimulation of glucokinase (GK), and inhibition of phosphoenolpyruvate carboxykinase (PEPCK) expression. Histopathological studies show the protective effect of herbal KGE on the integrity of the mice's pancreas. Molecular docking studies was performed in which the bio-active compound ethyl-p-methoxy cinnamate (EPMC) found in KGE shows good binding energy with the target protein AKT, GSK3, insulin receptor (IR), protein-tyrosine phosphatase 1B (PTB1B), GK, and PEPCK, indicating a good binding interaction. ADMET studies also revealed that EPMC has desirable physicochemical and pharmacokinetic properties and very low toxicity (LD 50 7900 mg/kg) compared to standard metformin (LD50 680 mg/kg). Herbal KGE and EPMC show a pleiotropic therapeutic effect and may be a potent adjunct and drug, respectively, in attenuating diabetes.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"247 - 267"},"PeriodicalIF":1.8,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of biological activities of in vitro grown Sesamum orientale plant extract on the cell cultures: wound healing and antiproliferation","authors":"Sevil Yeniocak,&nbsp;Selin Galatalı,&nbsp;İrem Demir,&nbsp;Aysel Uğur,&nbsp;Nurdan Saraç,&nbsp;Ergun Kaya","doi":"10.1007/s13596-024-00791-w","DOIUrl":"10.1007/s13596-024-00791-w","url":null,"abstract":"<div><p>Sesame has gained significant attention in the pharmaceutical industry for its antifungal, antiproliferative, antioxidant, and antiproliferative properties, as well as its use as a nutritional supplement. Additionally, sesame is a good source of vitamins B and E and has shown potential as a food that can lower serum cholesterol, balance blood pressure, and aid wound healing. In vitro grown <i>Sesamum orientale</i> L. cv. \"Gökova\" are presently undergoing tests to determine their cytotoxic, wound-healing, and antiproliferative properties using L929 fibroblasts, A549 lung epithelial, and MCF-7 breast cell lines. The IC₅₀ values for A549 were 922.73 g ml⁻¹ (extract) and 33.09 g ml⁻¹ (cisplatin), 1837.07 g ml⁻¹ (extract) and 19.27 g ml⁻¹ (cisplatin), and for L929 they were 154.70 g ml⁻¹ (extract). The subcytotoxic dosages of the extract demonstrated wound healing in L929 fibroblast cell cultures within 48 h. The transcripts of the <i>BCL-XL</i> gene, which exhibit an inverse correlation with the apoptotic pathway, and the transcripts of the <i>Cas3</i> and <i>Cas9</i>, which show a positive correlation with the apoptotic pathway, were scrutinized to ascertain the anti-cancer properties. The study revealed a significant increase in gene expression in A549 and MCF-7 lines after exposure to plant extract or cisplatin. The findings suggest that sesame extract can promote wound healing at lower doses and exhibit antiproliferative activity at higher doses. The study provides a promising foundation for the development of a pharmacological drug that offers wound-healing and/or antiproliferative effects. The product has potential applications across a range of future projects and can be utilised with numerous cell lines.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"233 - 246"},"PeriodicalIF":1.8,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The synthesis, application and therapeutic perspectives of medicinal plants-based solid lipid nanoparticles: a comprehensive review","authors":"MohammadSadeq Mottaqi,&nbsp;Mohammad Amin Langarizadeh,&nbsp;Neda Molaali,&nbsp;Masoud Rezaei,&nbsp;Alieh Ameri,&nbsp;Hamid Forootanfar","doi":"10.1007/s13596-024-00787-6","DOIUrl":"10.1007/s13596-024-00787-6","url":null,"abstract":"<div><p>Lipid-based drug carriers have gained massive amounts of interest in the last two-decade period as a way to deliver drugs that are not particularly water-soluble. Pharmaceutical uses of lipid nanocarriers include carrying and administering a wide range of therapeutic ingredients. Solid lipid nanoparticles (SLNs) were designed and formulated to rise above the disadvantages of existing colloidal systems, including microemulsions, liposomes and polymeric nanoparticles, by offering benefits such as a favorable release profile and targeted medication distribution while having great physical integrity. We sought to evaluate the recent findings on the current issue to provide a comprehensive perspective of the subject utilizing an extensive literature search to pinpoint the latest scientific reports on this subject. The focus of this paper is on the research employing SLNs for delivery of medicinal plants and testing them in vitro and in vivo. There are a number various standard variables affecting the release of compounds from SLNs throughout all administration routes. Further, the current paper comprehensively elucidates assorted prospects of SLNs. Next, the investigated SLNs encapsulating herbal compounds in scientific articles are reviewed in details. As many herbal compounds are hydrophobic substances, the SLNs encapsulating them can be beneficial in various therapeutic applications. The most studies herbal compounds are Curcumin, followed by Silibinin and Artemisinin.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"69 - 105"},"PeriodicalIF":1.8,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical, elemental, morphological and toxicological characteristics of traditional Indian Siddha formulation: Kasthuri Karuppu","authors":"N. Kannan,&nbsp;Anil Kumar NV,&nbsp;S. Balaji","doi":"10.1007/s13596-024-00790-x","DOIUrl":"10.1007/s13596-024-00790-x","url":null,"abstract":"<div><p>The chemical, elemental, morphological and toxicological characteristics of organometallic formulation (<i>Kasthuri Karuppu</i>) were assessed using UV-spectrophotometer, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, ICP-OES, Energy dispersive X-ray, XRD and Zeta potential. The physical properties such as size (10–80 nm), surface area (3.1 m²/g), surface charge (-36mV), and bandgap energy (3.6 eV) were correlated with reactivity. Based on the elemental distribution of Hg, As and S, the proportion favours HgS that is less toxic and reactive. There were no toxic effects of the tested formulation at concentration 60 µg/mL against normal and cancer cells (observed during 24 h). However, the increase in time duration (72 h) had lethal effects in both the cells. The developmental toxicity of the organometallic formulation was assessed in Zebrafish embryos. The safe dosage was found to be 100 µg/mL. The toxicity of the formulation in vitro and in vivo is based on the exposure time and dosage.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"221 - 231"},"PeriodicalIF":1.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-024-00790-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictors of high phytotherapy usage among women with cancer: the impact of cancer duration, symptoms, and psychosocial factors","authors":"Nadia Ouzennou ,&nbsp;Mohamed Aboufaras,&nbsp;Rajae Najib,&nbsp;Asmaa Lakhdissi,&nbsp;Karima Selmaoui","doi":"10.1007/s13596-024-00789-4","DOIUrl":"10.1007/s13596-024-00789-4","url":null,"abstract":"<div><p>Objective: Gynecological and Breast Cancers (GCBC) are extremely common worldwide, including in Morocco. Patients suffering from these forms of cancer often turn to complementary and alternative medicine (CAM), particularly phytotherapy. However, there has been no research on Moroccan patients using this medicine. We aim to assess the frequency and factors involved in the use of phytotherapy. Methods: This was a cross-sectional analytical study. We interviewed 317 patients in an oncology unit. We calculated the 12-month prevalence of herbal medicine use and identified predictors of herbal medicine use by multivariate analysis using SPSS software. Results: The prevalence of patients using phytotherapy was 40.7%. Patients used 48 plant species. The most commonly used plants were <i>Marrubium vulgare</i> L., <i>Allium cepa</i> L. and <i>Retama monosperma</i> (L.) Boiss. This use was associated with a cancer duration greater than one year (<i>p</i> &lt; 0.05; OR = 0.414), pain (<i>p</i> &lt; 0.01; OR = 2.711), anorexia (<i>p</i> &lt; 0.05; OR = 2.079), the social impact of the disease (<i>p</i> &lt; 0.01; OR = 4.140), the expected benefits of C&amp;CM (<i>p</i> &lt; 0.000; OR = 3.453), and the social norms of relatives (<i>p</i> &lt; 0.000; OR = 3.502). Conclusion: The use of herbal medicine as CAM among women with is highly prevalent. The user profile identified in this study deviates from the patterns observed in the international literature. Further research is warranted in different regions of Morocco, exploring various CAM modalities, and employing alternative methodological approaches. The use of herbal medicine as CAM in women with GCBC is widespread. The profile of users identified in this study deviates from patterns observed in the international literature. Further research is warranted in different regions of Morocco, exploring different CAM modalities and using other methodological approaches.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"485 - 494"},"PeriodicalIF":1.8,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of natural inhibitors for osteoarthritis targeting inflammatory pathway with pharmacoinformatics and molecular docking","authors":"Rok Su Shim,&nbsp;Fahad Hassan Shah,&nbsp;Young Seok Eom,&nbsp;Saad Salman,&nbsp;Song Ja Kim","doi":"10.1007/s13596-024-00782-x","DOIUrl":"10.1007/s13596-024-00782-x","url":null,"abstract":"<div><p><i>Ziziphus jujuba</i> is a traditional Chinese medicinal plant, whose fruit (<i>Jujubae fructus</i>) has been highly utilized in herbal medicine for decades. The fruit of this plant contains a wide variety of phytochemicals having efficacious biological activity. These phytochemicals have been utilized in this study to discover potential drug candidates in preventing osteoarthritis (OA) progression using network pharmacology and molecular docking method. The phytochemicals information of <i>Jujubae fructus</i> was obtained from the TCMSP database, which were screened for druglikeness and oral bioavailability. Screened compounds were explored for Network pharmacology using BATMAN-TCM database and Molecular Docking with IGEMDOCK 2.1. Obtained compounds from this analysis were used to predict their ADMET properties. Network pharmacology analysis showed that Mairin, Oleanonic acid, Oleanolic acid, and Stigmasterol reduce inflammation and activate Peroxisome Proliferator-Activated Receptors (PPARs) signaling pathway to intervene with OA progression. Molecular docking predicted that Mairin, Oleanonic acid, and Stigmasterol reduce inflammation by targeting Janus-kinase-2 protein. All these compounds have moderate ADMET qualities, which need to be calibrated to counteract their toxic effect and increase the drug effectivity. Mairin, Oleanonic acid, and Stigmasterol are the potential phytochemicals of <i>Jujubae fructus</i> playing a therapeutic role in OA. These compounds can be further explored for invitro and clinical studies.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"211 - 219"},"PeriodicalIF":1.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer activity of thymoquinone and its combinations with doxorubicin and linseed oil in the treatment of xenograft tumors","authors":"Oleg Shadyro,&nbsp;Anna Sosnovskaya,&nbsp;Irina Edimecheva,&nbsp;Lana Kireicikova,&nbsp;Svetlana Samovich,&nbsp;Boris Dubovik,&nbsp;Sergei Krasny,&nbsp;Dmitry Tzerkovsky","doi":"10.1007/s13596-024-00785-8","DOIUrl":"10.1007/s13596-024-00785-8","url":null,"abstract":"<div><p>Thymoquinone (TQ), the main bioactive component of Nigella sativa, has shown promising anticarcinogenic activity in extensive preclinical studies. This study aimed to evaluate the antitumor activity of TQ, in a wide range of doses with monotherapy and combined use with known the chemotherapeutic drug doxorubicin (Dox) and linseed oil (LO) in a rat model with Pliss lymphosarcoma (PLS) and a mouse model with Lewis lung adenocarcinoma (LLC). Animals was administered orally of TQ daily for 12 days at doses of 5, 10 and 30 mg/kg of body weight (rats) and 5 mg/kg (mice) from the 7th day after subcutaneous transplantation of tumors, as well as combinations of TQ with Dox (5 mg/kg, once at the start of treatment by i.p.) and LO (3 ml/kg, orally). It was found that TQ in the studied doses significantly suppresses the growth of the PLS and the LLC tumors (<i>p</i> &lt; 0.001) and increases the frequency of complete tumor regression (FCR) (<i>p</i> &lt; 0.001) compared to control. TQ, especially (TQ + LO) were able to effectively potentiates the antitumor effect of Dox when used in combination, reducing the PLS tumor volume at the end of treatment by 1.9–2.7 times (<i>p</i> &lt; 0.009), increasing the FCR tumors 60 days after the start of treatment by 2.7–3.7 times (<i>p</i> &lt; 0.02) (PLS) and by 1.5 times (<i>p</i> ≤ 0.05) (LLC), as well as reducing the LLC frequency of metastasis compared to Dox monotherapy. The results strongly suggest that TQ and its combination with LO have clinical potential as an adjuvant in cancer chemotherapy using Dox.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"197 - 209"},"PeriodicalIF":1.8,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-fatigue effects of low-molecular-weight brewer’s yeast extracts on mice","authors":"Minsu Pyeon,&nbsp;Jiwon Lee,&nbsp;Myungmi Moon,&nbsp;Jeonghoon Park,&nbsp;Ahyuk Kim,&nbsp;Seonyoung Im,&nbsp;Sooan Kim,&nbsp;Junho H. Lee,&nbsp;Gyung-Hyun Jo","doi":"10.1007/s13596-024-00786-7","DOIUrl":"10.1007/s13596-024-00786-7","url":null,"abstract":"<div><p>Muscle mass naturally declines with age; however, sudden and significant muscle loss is considered a pathological condition. This decline in physical function increases the risk of falls and fractures; additionally, maintaining daily life activities can become challenging, leading to further complications. The World Health Organization has classified sarcopenia as a disease because affected individuals exhibit a mortality rate up to double that of those without the condition. Brewer’s yeast, a traditional nutritional supplement, has garnered recent attention for its potential role in muscle synthesis. This study focuses on the efficacy of low-molecular-weight yeast extract, specifically that with a molecular weight &lt; 10 kDa, in enhancing muscle growth and regeneration. Yeast extracts are known for their high bioavailability and capacity to facilitate efficient absorption and utilization within the body. Our research involved a series of in vitro and in vivo experiments aimed at elucidating the effects of these extracts on muscle cells. The results demonstrated a significant increase in protein synthesis within muscle cells treated with low-molecular-weight yeast extracts. Additionally, these extracts promoted muscle cell growth and differentiation, further supporting their role in muscle development. Detailed analysis revealed that the yeast extracts enhanced the activation of key signaling pathways involved in muscle protein synthesis and cell differentiation. In the rota-rod test, all yeast extract-treated groups exhibited an increasing trend in exercise performance compared with the control group. These findings underscore the potential of low-molecular-weight yeast extracts as a valuable component in muscle synthesis and regeneration. Furthermore, this study highlights the broader implications of these findings for the growing older population. In addition, it suggests that low-molecular-weight yeast extracts can be incorporated into dietary supplements aimed at addressing sarcopenia and muscle loss in older adults by enhancing muscle growth and recovery.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"475 - 484"},"PeriodicalIF":1.8,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of acupressure on migraine: a systematic review and meta-analysis","authors":"Arya Asadizeidabadi,&nbsp;Seyedmohammadamin Hosseini,&nbsp;Ali Ataei,&nbsp;Hamidreza Amiri,&nbsp;Daniel Shafiei,&nbsp;Mandana Dehghan,&nbsp;Seyede Zakieh Modares Mosalla","doi":"10.1007/s13596-024-00780-z","DOIUrl":"10.1007/s13596-024-00780-z","url":null,"abstract":"<div><h3>Introduction</h3><p>Migraine, a prevalent neurological disorder, affects about 14.4% of the global population, ranking among the leading causes of disability, especially in young women. Acupressure, a non-invasive therapy akin to acupuncture, has emerged as a potential intervention for migraine management. However, its effectiveness remains inconclusive due to the lack of comprehensive systematic reviews. This study aims to systematic review and meta-analysis existing literature to investigate the effect of acupressure on migraine symptoms. <i>Methods</i> Adhering to PRISMA guidelines, a comprehensive search strategy was employed across multiple databases. Eligible studies included randomized controlled trials (RCTs), cohort studies and case reports involving individuals diagnosed with various migraine types. Data synthesis and analysis were conducted using STATA 14, with meta-analyses performed on available quantitative data. <i>Results</i> From 250 initial records, 12 studies were included, comprising ten RCTs, one cohort study, and one case report, with six RCTs included in the meta-analysis. Results showed mixed findings regarding acupressure’s impact on migraine symptoms, with improvements noted in headache intensity, frequency, duration, and nausea. However, substantial heterogeneity among studies and methodological limitations were identified, limiting the conclusiveness of the evidence. <i>Conclusion</i> Despite promising results, this review underscores the need for further research. Methodologically robust studies with larger sample sizes are warranted to elucidate acupressure’s role in migraine treatment. Nonetheless, continued investigation into complementary therapies like acupressure holds promise for providing non-invasive options for migraine management.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"385 - 393"},"PeriodicalIF":1.8,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethanol extract of black shallot (Allium ascalonicum Linnaeus) for breast cancer prevention: evidence from a DMBA-induced mouse model","authors":"Thi Phuong Nhung Tran,&nbsp;Thi Thanh Nha Tran","doi":"10.1007/s13596-024-00781-y","DOIUrl":"10.1007/s13596-024-00781-y","url":null,"abstract":"<div><p>Breast cancer is the leading cause of cancer-related mortality, posing challenges in treatment due to high costs, numerous side effects, and systemic toxicity. Consequently, many patients seek natural compounds with low toxicity and potential anticancer and pain-relief properties. The ethanol extract of black shallot (EEBS) from <i>Allium ascalonicum</i> Linnaeus has shown promise due to its antioxidant properties and ability to induce apoptosis in various cancer cell lines. This study investigates the effect of EEBS on breast cancer prevention in a DMBA-induced mammary tumor model in mice. EEBS was administered at doses of 300 to 500 mg/kg body weight (BW), with tamoxifen (3.3 mg/kg BW) serving as a standard reference drug. DMBA-treated mice were confirmed to develop mammary tumors (<i>p</i> &lt; 0.05). In contrast, mice dosed with EEBS exhibited slower tumor progression (<i>p</i> &lt; 0.05), indicating the extract’s promising therapeutic potential. EEBS significantly reduced tumor volumes, incidence rates, and TNF-α levels (<i>p</i> &lt; 0.05) in a dose-dependent manner, achieving a maximum reduction in tumor volume of 65.8% and a minimum incidence rate of 24 ± 5.48% at the dose of 500 mg/kg. Treatment with EEBS also considerably improved hematological parameters (WBC, LYM, RBC, HGB, HCT, and PLT) and reduced hepatic and renal function indicators (AST, ALT, ALP, GLU, BIL, URE, CRE, URA) (<i>p</i> &lt; 0.05), reflecting its protective effects on liver and kidney functions. Histopathological examination further confirmed the efficacy of EEBS in inhibiting tumor progression. Importantly, no mortality was observed, highlighting the safety of the experimental treatments. These findings suggest that black shallot extract could be a potential therapeutic agent for breast cancer prevention and for mitigating DMBA-induced toxicity.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"459 - 474"},"PeriodicalIF":1.8,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}