Advances in Traditional Medicine最新文献

{"title":"The biological effects of Rosa rugosa extract on keratinocyte differentiation and enhancement of skin barrier function","authors":"Minju Jeong,&nbsp;Jeongmin Cho,&nbsp;Dohyo Lim,&nbsp;Myungsoo Choi,&nbsp;Yukyung Park,&nbsp;Yeonhee Cheong,&nbsp;Youngwoong Kang,&nbsp;Inhee Kang,&nbsp;Sooah Kim,&nbsp;Dongwon Kim","doi":"10.1007/s13596-024-00778-7","DOIUrl":"10.1007/s13596-024-00778-7","url":null,"abstract":"<div><p>We investigated the biological activity of <i>Rosa rugosa (R. rugosa</i>) extract in human epidermal keratinocytes. To assess the antioxidant capacity of the extract, various concentrations (0.1, 0.5, 1, 5, 10, 50 mg/ml) were tested against DPPH and ABTS free radicals. The results showed antioxidant activities of 45% at 5 mg/ml, 60% at 10 mg/ml, and 82% at 50 mg/ml. Cell toxicity experiments using human keratinocytes revealed no cytotoxicity up to 10 μg/ml, and the cells exhibited a differentiated morphology. We confirmed the efficacy of the extract in keratinocytes, with a significant increase in the expression of keratinocyte differentiation factors Keratin (KRT)1 and KRT10. Moreover, the expression of genes related to skin barrier function, including Filaggrin (FLG), Involucrin (IVL), Loricrin (LOR), and Claudin1 (CLDN1), significantly increased. In an in vitro atopic model experiment treating human keratinocytes with Interleukin (IL)-4/IL-13, the <i>R. rugosa</i> extract maintained the expression of FLG protein. Overall, <i>R. rugosa</i> demonstrated high antioxidant activity and was found to be a safe material for human keratinocytes. Furthermore, it induced keratinocyte differentiation, particularly increasing the expression of FLG, IVL, LOR, and CLDN1, which are components of the skin barrier. While additional research is needed to validate these experimental results, <i>R. rugosa</i> extract holds promise as a novel ingredient for the development of cosmetics or pharmaceuticals that could alleviate inflammatory skin conditions such as atopic dermatitis or psoriasis.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"451 - 457"},"PeriodicalIF":1.8,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer potential of Calligonum comosum L.’Her. and Calligonum crinitum Boiss plants from United Arab Emirates","authors":"Wesam Khader,&nbsp;Mohamed K. Kayali,&nbsp;Tawfik M. Taj Eddin,&nbsp;Abdul Hadi S. S. S. Alameri,&nbsp;Mughair Abdul Aziz,&nbsp;Jaleel Kizhakkayil,&nbsp;Annadurai Senthilkumar,&nbsp;Ayesha S. Al Dhaheri,&nbsp;Abdul Jaleel","doi":"10.1007/s13596-024-00773-y","DOIUrl":"10.1007/s13596-024-00773-y","url":null,"abstract":"<div><p>Indigenous plants from arid regions are known for their diverse applications in traditional medicine. These plants are particularly promising as potential anticancer agents due to their ability to produce a wide range of secondary metabolites. The present study addressed the problem of identifying and evaluating the anticancer properties of crude extracts from indigenous arid-region plants, <i>Calligonum comosum</i> L.’Her. and <i>Calligonum crinitum</i> Boiss., to determine their efficacy against human breast (MCF-7) and human colon adenocarcinoma (Caco-2) cell lines. Through various solvent-treated leaf extracts, including hexane, methanol, acetone, and chloroform, we aimed to determine the antiproliferative activities and identify extracts with promising efficacy in inhibiting cancer cell viability. The morphological changes and anticancer effects induced by these solvent-treated <i>C. comosum</i> and <i>C. crinitum</i> leaf extracts were investigated. Our results indicated that the use of acetone isolated leaf extracts on the MCF-7 cells displayed apoptotic morphological changes. Similarly, the MTT cytotoxicity assay revealed that the acetone-treated extracts of <i>C. comosum</i> and <i>C. crinitum</i> showed a significant inhibitory effect on MCF-7 cell lines, with IC₅₀ values of 24.62 μg/ml and 23.41 μg/ml, respectively. Conversely, the other solvents resulted in higher IC₅₀ values for inhibiting the MCF-7 cell proliferation. While for the Caco-2 cell lines, we observed remarkable antiproliferation activity, with IC₅₀ values of 37.76 µg/ml and 74.88 µg/ml for the <i>C. comosum</i> and <i>C. crinitum</i> chloroform-isolated extracts, respectively, in comparison to the hexane, methanol, and acetone-isolated extracts. These findings suggest that the extracts of <i>C. comosum</i> and <i>C. crinitum</i> possess significant potential for inhibiting the viability of MCF-7 and Caco-2 cancer cell lines, indicating their possible use as anticancer agents.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"439 - 450"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the anti-inflammatory potential of zingiber officinale on adjuvant-induced arthritis","authors":"Great Iruoghene Edo,&nbsp;Favour Ogheneoruese Onoharigho,&nbsp;Khawla A. Kasar,&nbsp;Irene Ebosereme Ainyanbhor,&nbsp;Agatha Ngukuran Jikah","doi":"10.1007/s13596-024-00779-6","DOIUrl":"10.1007/s13596-024-00779-6","url":null,"abstract":"<div><p>Ginger <i>(Zingiber officinale)</i> is an herb utilized all over the world for its extensive phytochemical properties. This study aims to evaluate the anti-inflammatory effects of <i>Zingiber officinale</i>. Arthritis was induced in the rats used in the experiment using Complete Freund’s adjuvant (CFA). Gas chromatography-Mass spectroscopy (GC-MS) was used to screen for bioactive components present in ginger. For ten days, <i>Zingiber officinale</i> ethanolic extract (62.5, 125, and 250 mg/kg/day) was administered orally to the rats. Edema in the paws was measured before and 10 days after <i>Zingiber officinale</i> treatment in order to identify pathological alterations. Additionally, cytokine levels (TNF-α, IL-1β, and IL-6) were assessed in plasma. When compared to the control group, the CFA-arthritis induced group had edema in their paws and knee joints considerably reduced upon treated with <i>Zingiber officinale</i> at days 6, 8, and 10. Rats in the groups given <i>Zingiber officinale</i> also showed a significant decrease in cytokine levels and also recovered from the pathological alterations brought on by CFA. Twenty bioactive metabolites were found when the extracts of the ethanol, hexane, and chloroform was analyzed using GC-MS. There were no carbohydrates or steroids in the chloroform and n-hexane extract. Protein and cardiac glycoside were also missing. In summary, our findings demonstrated that <i>Zingiber officinale</i> treatment was successful in preventing CFA-induced arthritis through its cytokine level regulation anti-inflammatory property.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"187 - 196"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer effect of ethanolic extract of Rheum ribes L. rhizomes: assessment of dose and time-dependent effects in cell-based models","authors":"Zeina Nehme,&nbsp;Fatima Ghandour,&nbsp;Akram Hijazi,&nbsp;Mona Diab-Assaf,&nbsp;Racha Karaky","doi":"10.1007/s13596-024-00772-z","DOIUrl":"10.1007/s13596-024-00772-z","url":null,"abstract":"<div><h3>Introduction</h3><p>Medicinal plants have been traditionally used in the treatment of various diseases including cancer, however, their IC₅₀ determined in vitro are hardly attained in patients. <i>Rheum ribes L</i>. is an indigenous medicinal plant shown to possess anticancer activity due to high polyphenolic compounds abundance. Our objective was to assess if long-term exposure to plant extract concentrations lower than IC₅₀ may still possess an anticancer potential.</p><h3>Methods</h3><p>We used the ethanolic extract of rhizomes of <i>Rheum ribes L.</i> (EERR) and assessed its effect on proliferation, clonogenicity, and senescence in cell lines-based models.</p><h3>Results</h3><p>Our results showed that EERR has a short-term antiproliferative activity on non-small cell lung carcinoma A549, breast cancer MCF7, and glioblastoma SF268 cell lines with IC₅₀ varying from 100 to 255 µg/mL. Additionally, EERR decreased the population doubling level and the clonogenic ability of the three cancer cell lines at lower concentrations (10 to 100 µg/mL) and with longer treatment protocols. This was accompanied by a significant increase in the senescence-associated β-galactosidase activity, suggesting that EERR induces senescence at these concentrations.</p><h3>Conclusion</h3><p>EERR displayed anticancer activity at concentrations 2- to 10-fold lower than the IC₅₀ in three different cancer cell lines and should be further investigated as it may provide novel therapeutic avenues for cancer treatment.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"175 - 185"},"PeriodicalIF":1.8,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jatropha tanjorensis leaf extracts attenuate adrenaline-induced myocardial cell injury via modulation of cardio-inflammatory biomarkers in Wistar rats","authors":"Idara Asuquo Okon,&nbsp;Elizabeth Bassey Umoren,&nbsp;Emmanuel Uchechukwu Modo,&nbsp;Providence Idabie Brown,&nbsp;Joseph Igbo Enya,&nbsp;Daniel Udofia Owu","doi":"10.1007/s13596-024-00776-9","DOIUrl":"10.1007/s13596-024-00776-9","url":null,"abstract":"<div><p>Myocardial injury (MI) has remain a global concern due to high mortality rate associated with cardiovascular diseases. <i>Jatropha tanjorensis (J. tanjorensis)</i> is a medicinal herb with proven medicinal properties. This study investigated the level of cardio-inflammatory response in adrenaline-induced MI in rats treated with <i>J. tanjorensis</i>. Twenty Wistar rats were randomly divided into four groups (<i>n</i> = 5). Group 1 served as Control. Group 2 was induced with MI using 2 mg/kg bodyweight of adrenaline administered subcutaneously for two days at 24 h interval between the first and second administrations. Group 3 received 200 mg/kg body weight of methanol extract of <i>J. tanjorensis</i> orally for 14days while Group 4 were induced with MI using 2 mg/kg body weight of adrenaline and treated with 200 mg/kg of <i>J. tanjorensis</i> leaf extract for 14 days. Blood was collected via cardiac puncture for biochemical analysis. <i>J. tanjorensis</i> reduced adrenaline-induced MI by lowering serum concentration of ALT, AST and ALP, pro-inflammatory (MDA, CRP and IL-6) and cardiac injury markers (CK-MB, Troponin-I). SOD and NO level were also raised in <i>J. tanjorensis</i>-treated animals. The extract also restored histoarchitectural changes in the cardiac muscle. <i>Jatropha tanjorensis</i> mitigates cardio-inflammatory response and restores cyto-architecture of the cardiac muscle in adrenaline-induced MI rats.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"165 - 173"},"PeriodicalIF":1.8,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141798698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxicity and apoptotic effect of Methanolic extract of Red Sea seagrass, Enhalus acoroides (Linn.f.) Royle, against hepatocellular carcinoma cell line (HepG-2) and phytochemical characterization","authors":"Shimaa I. A. Mohamed,&nbsp;Amgad El Shaffai,&nbsp;Shaymaa M.M. Yahya,&nbsp;Walaa S.A. Mettwally,&nbsp;Ghada H. Elsayed","doi":"10.1007/s13596-024-00775-w","DOIUrl":"10.1007/s13596-024-00775-w","url":null,"abstract":"<div><p>Hepatocellular carcinoma is considered as one of the most serious cancer types. The main objective of this research is to explore <i>Enhalus acoroides (EA)</i> (L.f.) Royle’s cytotoxic effects and methods of action on HepG-2 (hepatocellular carcinoma cell line). For the purpose of identifying the phenolic compounds in EA, high performance liquid chromatography (HPLC) was applied. Using the MTT assay, the anti-proliferative effects on HepG-2 were assessed. The cell cycle arrest and apoptotic processes were investigated using flow cytometry. An analysis was performed to determine how seagrass influenced the mitochondrial membrane potential (ΔψM). The impact of Caspases 3/7 was evaluated using the colorimetric assay. Finally, the expression level of gene expression using quantitative RT-PCR was investigated. The seagrass alcoholic extract effectively and concentration-dependently reduced the growth of HepG-2 cells without inflicting any adverse effects to normal human skin fibroblast HSF. EA primarily triggers apoptosis which results in cell death. A significant decrease in the G0/G1 phase and an increase of cells at S phase were indicators of the S phase cell cycle arrest. The results reflected the depletion in (ΔψM) in HepG-2 dose-dependently. Following therapy for 24 h, caspase 3/7 activities were enhanced. In conclusion, a quantitative real-time RT-PCR investigation for EA seagrass on HepG-2 demonstrated downregulated expression levels of the survival, BCL-2, CDC-2, and CC2DA1 genes. Collectively, the seagrass <i>EA</i> shows great promise as a hepatocellular cancer treatment and possesses the capacity to induce apoptosis in HepG-2.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><img></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"425 - 437"},"PeriodicalIF":1.8,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141807493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative efficacy of four potent bioactive molecules of fenugreek seed in holistic management of type 1 diabetes","authors":"Jayjeet Majumdar,&nbsp;Aniruddha Chatterjee,&nbsp;Suman Bhusan Chakraborty,&nbsp;Pratip Chakraborty,&nbsp;Supriti Sarkar","doi":"10.1007/s13596-024-00774-x","DOIUrl":"10.1007/s13596-024-00774-x","url":null,"abstract":"<div><p>Fenugreek seed extract (FSE) is known to possess antidiabetic properties. The current study evaluated the comparative potency of 4 different components of FSE (Trigonelline, 4-Hydroxyisoleucine, Diosgenin, and Galactomannan) in amelioration of diabetes-induced hyperglycaemia; metabolic and stress disorders. FSE and the four bioactives were fed to normal and alloxan-induced type-1 diabetic rats for four weeks. Changes in pancreatic hormones, lipid profile, antioxidant enzymes, and expression of Glucose transporter-2 (GLUT-2) and Glucagon-like peptide-1 receptor (GLP-1R) were evaluated. Key findings revealed the highest levels of serum insulin and GLP-1; islet cell regeneration, increased Dipeptidyl peptidase-IV enzyme inhibition, significant recovery of GLUT-2 and GLP-1R expression, and maximum levels of low-density lipoprotein receptors (LDLR) in trigonelline fed diabetic rats. The same bioactive also caused the maximum increase in various antioxidant enzymes with decreased serum malondialdehyde (MDA) levels in diabetic rats. The 4-Hydroxyisoleucine showed antidiabetic properties either equal to or less significantly as compared to trigonelline. Diosgenin on the other hand caused maximum stimulation only in lipid metabolism whereas galactomannan couldn’t produce significant changes in any parameters over the other 3 components. The results indicated trigonelline is the most desirable component of FSE and may be used as a phytotherapeutic agent for the holistic management of type-1 diabetes.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 2","pages":"411 - 424"},"PeriodicalIF":1.8,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141822149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro and in-silico evaluation of anticancer potential of Oxalis corniculata","authors":"Meghna Ravishankar Valakatte,&nbsp;Vibha Viswanath,&nbsp;Manasa Aithal,&nbsp;Prashantha Karunakar,&nbsp;Shanti Koppala Narayanappa","doi":"10.1007/s13596-024-00777-8","DOIUrl":"10.1007/s13596-024-00777-8","url":null,"abstract":"<div><p>Plant secondary metabolites have been gaining significant attention as potential cancer therapeutics in recent years. In several cases, these are present in plants that are commonly found in the surrounding environment. This study explores the anticancer properties of the methanolic extract of <i>Oxalis corniculata</i>, a common creeper plant known to have medicinal properties. The phytochemical characterization of the extract was performed by biochemical tests and gas chromatography mass spectrometry (GCMS). Cytotoxicity studies on the HCT-116 cell line have shown that the extract can inhibit cell proliferation with an IC₅₀ value of 119.498 µg/mL. Through molecular docking and molecular dynamics simulation, it has been observed that two constituents of the extract namely Desulphosinigrin and d-Glycero-d-ido-heptose exhibit strong and specific interactions with the caspase binding site of the X-linked inhibitor of apoptosis protein (XIAP). Thus, these compounds may be capable of inhibiting XIAP in the cellular environment, thereby promoting apoptosis, resulting in the death of cancer cells.</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 4","pages":"1229 - 1239"},"PeriodicalIF":1.8,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141644104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review on pleiotropic effects and therapeutic potential of soy lecithin","authors":"Shubhada V. Mangrulkar,&nbsp;Sayli S. Kulkarni,&nbsp;Pratiksha V. Nanepag,&nbsp;Pankaj S. Neje,&nbsp;D. R. Chaple,&nbsp;Brijesh G. Taksande,&nbsp;Milind J. Umekar","doi":"10.1007/s13596-024-00770-1","DOIUrl":"10.1007/s13596-024-00770-1","url":null,"abstract":"<div><p>Lecithin is one of the most useful and valuable by-products of the oilseed industry and has long been a crucial component of a wide range of both food and non-food items. Lecithin obtained from soybean (Soy) is called soy lecithin and is composed of triglycerides, fatty acids, pigments, sterols, steroid glycosides, esters, tocopherols, and carbohydrates. Lecithin serves a variety of industrial purposes in food as well as non-food industries. Soy lecithin has grown in importance as a component of nutraceuticals and food supplements during the past few decades. Soy lecithin primarily consists of phospholipids including phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS) which plays a major role in biological and structural processes such as cellular signalling and membrane transport. Soy lecithin has exceptional biocompatibility and is amphipathic in nature. Because of these special qualities, soy lecithin is best suited to be used as a major pharmacological excipient, and it is broadly used in drug delivery systems. It also has a significant role in medicine as it is an antioxidant, increases biliary secretions, maintains cholesterol levels, storehouse of choline, and supports the synthesis of crucial neurotransmitters involved in memory recall and storage. The core intent of this review is to investigate and update the information on the therapeutic importance of soy lecithin and highlight its various other commercial roles in the pharmaceutical industries and food industries.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"25 1","pages":"145 - 164"},"PeriodicalIF":1.8,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141650641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of a traditional herbal formulation (Azaraghi Majoon) on opium use disorder in male rats","authors":"Tayyebe Tavakoli,&nbsp;Leila Mohammad Taghizadeh kashani,&nbsp;Meysam Shirzad,&nbsp;Mohammad Reza Memarzadeh,&nbsp;Ali Shamsizadeh,&nbsp;Ayat Kaeidi,&nbsp;Jalal Hassanshahi,&nbsp;Mahsa Hassanipour","doi":"10.1007/s13596-024-00771-0","DOIUrl":"10.1007/s13596-024-00771-0","url":null,"abstract":"<div><p>Opium Use Disorder (OUD) has the highest rate of opioid use disorder in Iran. The present study was designed to investigate the effect of Azaraghi Majoon (AM), a traditional Persian compound, on a rat model of OUD. AM was prepared from a combination of 14 herbs plus honey, standardized by strychnine and brucine using high-performance liquid chromatography, and administered by gavage. One hundred twenty male mice were used to investigate the effect of AM on the manifestations of opium withdrawal syndrome and craving for opium. Naloxone-precipitated withdrawal signs and conditioned place preference (CPP) test were used to assess the scopes mentioned above of OUD, respectively. A modified schedule of opium dependence was used to assess physical dependence (last for eight days). 50 mg/ml/kg intraperitoneal opium and 50, 100, or 150 mg/ml/kg gavaged AM in different groups were administered in the CPP paradigm. In the CPP test, treating opium-addicted animals with AM (50 and 150 mg/kg for time and 50, 100, and 150 mg/kg for frequency) resulted in the extinction of preference for drug compartments. Also, the administration of AM decreased the number of jumping, diarrhea, rearing, and grooming following naloxone-precipitated opium withdrawal signs. As AM could improve both physical and psychological withdrawal signs of opium use disorder in rats in this study, the authors suggest consideration of clinical trials to investigate possible beneficial effects of AM in OUD patients.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 4","pages":"1215 - 1227"},"PeriodicalIF":1.8,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141672115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}