Advances in Traditional Medicine最新文献

{"title":"Evaluation of the Modifying impact of myrtenol on intrahepatic cholestasis and extrahepatic cholestasis in male rats: role of oxidative stress and inflammation","authors":"Mohammad Amin Rajizadeh,&nbsp;Masoumeh Ghasemi,&nbsp;Mohammad Abbas Bejeshk,&nbsp;Mohammad Akhbari,&nbsp;Shadan Saberi,&nbsp;Fatemeh Bagheri,&nbsp;Sedigheh Amiresmaili","doi":"10.1007/s13596-025-00857-3","DOIUrl":"10.1007/s13596-025-00857-3","url":null,"abstract":"<div><p>Cholestatic liver disease occurs due to deficiencies in bile production, secretion, and passage via the biliary system. Cholestatic liver is therapeutic challenge due to limited treatment options and incomplete understanding of its pathophysiology. Myrtenol, which exists in the essential oils of some medicinal plants such as <i>Myrtus communis, Artemisia</i> and <i>Eucalyptus</i>—has demonstrated anti-inflammatory, antioxidant. Our aim of this study was to investigate the effect of myrtenol on liver damage in male rats with intrahepatic and extrahepatic cholestasis. Forty-eight male Wistar rats were randomly divided into two main groups: intrahepatic and extrahepatic cholestasis groups. In the intrahepatic cholestasis group, intrahepatic injury was elicited by intraperitoneal (IP) injection of acetaminophen (500 mg/kg) and included four subgroups: 1. Sham, 2. Acetaminophen (Aceta), 3. Dimethyl sulfoxide (Aseta + DMSO), and 4. Myrtenol (Aseta + Myr). The extrahepatic cholestasis group was induced by common bile duct litigation (BDL) and included four subgroups: (1) Sham, (2) BDL, (3) DMSO, and (4) Myrtenol (BDL + Myr). Myrtenol was administered intraperitoneally for six days. Blood samples and liver samples, were taken for biochemical and histological evaluations. The findings showed that Myrtenol significantly attenuated the increase in liver enzymes, pro-inflammatory cytokines, and oxidant levels caused by intra- and extra-hepatic cholestasis. Myrtenol also increased the decrease in hepatic antioxidant biomarkers. Myrtenol shows promise in reducing cholestatic liver injury by reducing liver enzymes, inflammation, and oxidative stress while restoring antioxidant defenses. However, further preclinical and clinical studies are necessary to confirm its therapeutic potential for human cholestatic diseases.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 2","pages":"433 - 447"},"PeriodicalIF":1.3,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148060692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oligomeric flavonoids from Cyperus rotundus tubers suppress growth, induce apoptosis, and boost antioxidant defense in multiple cancer cell lines","authors":"Amer Talib Tawfeeq,&nbsp;Zaynab Saad Abdulgany,&nbsp;Noah Abdulkhder Mahmood,&nbsp;Esraa Jaafar Saheb,&nbsp;Mohammed Saleh Abbas","doi":"10.1007/s13596-025-00855-5","DOIUrl":"10.1007/s13596-025-00855-5","url":null,"abstract":"<div><p>Medicinal plants have long held therapeutic value in traditional medicine, though their underlying biological mechanisms are often underexplored. The tubers of <i>Cyperus rotundus</i> are rich in bioactive flavonoids, and previous studies confirm their diverse phytochemical composition. We hypothesize that the total oligomeric flavonoid (TOF) fraction from <i>C. rotundus</i> exerts selective anticancer effects via a synthetic lethality-like mechanism—targeting malignant cells while sparing normal ones. This study investigated TOF’s cytotoxic and mechanistic effects on six cancer cell lines: human glioblastoma (AMGM), mouse mammary adenocarcinoma (AMN3), breast cancer (MCF7 and AMJ13), ovarian cancer (SKOV3), cervical carcinoma (HeLa), and rhabdomyosarcoma (RD). Two normal cell lines—rat embryo fibroblasts (REF) and African green monkey kidney cells (Vero)—served as controls. TOF was applied at concentrations of 50–500 µg/mL over 24, 48, and 72 h. Radical scavenging capacity was assessed via DPPH assay, with 100, 200, and 300 µg/mL TOF reducing DPPH by 59.56%, 78.9%, and 93.55% relative to ascorbic acid, confirming strong free radical scavenging activity. MTT assays revealed antiproliferative effects with IC₅₀ values ranging from 253.29 µg/mL (AMJ13 at 72 h) to 8620.68 µg/mL (VERO at 24 h). Cytopathological changes were visualized using crystal violet staining. Apoptosis was confirmed by acridine orange/propidium iodide dual staining, mitochondrial membrane potential disruption, DNA fragmentation, and comet assay—all indicating selective apoptosis in cancer cells while preserving normal cell integrity. Total antioxidant status (TAS) analysis showed increased activity in extracellular fractions post-treatment and reduced activity intracellularly, with the most significant shifts observed in AMJ13 and HeLa cells. Overall, TOF demonstrated potent anticancer properties through apoptosis induction and oxidative stress modulation. These findings support its potential as a selective, plant-derived therapeutic candidate and justify further investigation toward safer, targeted cancer treatments.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 2","pages":"409 - 432"},"PeriodicalIF":1.3,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148061264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo anti-rheumatoid arthritis activity and mechanistic insights of methanol extract of Zanthoxylum usambarense Engl.","authors":"James Kimani Kamau,&nbsp;Mathew Piero Ngugi,&nbsp;Joseph J. N. Ngeranwa","doi":"10.1007/s13596-025-00856-4","DOIUrl":"10.1007/s13596-025-00856-4","url":null,"abstract":"<div><p>This study aimed to determine the in vivo anti-rheumatoid arthritis potential and mechanistic insights of methanol extract of <i>Zanthoxylum usamberense</i>. Thirty rats were randomly classified into normal control, rheumatoid arthritis (RA) control, methotrexate control, and extract-treated (100, 200, and 300 mg/kg body weight) groups. RA was induced using complete Freund’s adjuvant. All treatments were administered orally for 21 days from day 8 post-induction of RA. On day 29, the rats were euthanized, and blood was drawn for hematological parameters analysis. The ankle joint tissue and liver were excised and utilized in gene expression and antioxidant assays, respectively. Phytochemical screening was undertaken using GC–MS and LC–MS. The effect of the extract significantly reduced levels of MDA, CRP, WBCs, and ESR; arthritis scores; ankle joint edema; and indices of spleen and thymus compared to RA control (<i>p</i> &lt; 0.05). The extract also significantly enhanced activities of SOD and GPx and levels of RBCs and Hb relative to RA control (<i>p</i> &lt; 0.05). Further, the extract significantly downregulated levels of <i>NF-κB</i>, <i>IL-1β</i>, <i>IL-6</i>, <i>MMP-3, RANKL</i>, and <i>TNF-α</i> genes, as well as upregulated expression of <i>I-κBα</i>, <i>IL-4</i>, and <i>IL-10</i> genes compared to RA control (<i>p</i> &lt; 0.05). The LC–MS analysis identified phytocompounds such as dihydroberberine, gamma-sanshool, laudanine, dihydrochelerythrine, ochotensine, and quercetin. The GC–MS screening identified phytochemicals such as methyl palmitate, sandaracopimara-8(14),15-diene, and stanozolol. These phytocompounds have been established to exert anti-rheumatoid arthritis effects. In conclusion, the extract exhibits potent anti-rheumatoid arthritis activity and may be a potential candidate in developing alternative agents against RA.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 2","pages":"391 - 407"},"PeriodicalIF":1.3,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148060941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flavonoids and phenols of Sclerocarya birrea fruit exocarp extracts differentially modulating antioxidant activities","authors":"Phazha Bushe Baeti,&nbsp;Keagile Bati,&nbsp;Phenyo Donald Brown,&nbsp;Runner Majinda,&nbsp;Kabo Masisi,&nbsp;Goabaone Gaobotse,&nbsp;Tebogo Elvis Kwape","doi":"10.1007/s13596-025-00854-6","DOIUrl":"10.1007/s13596-025-00854-6","url":null,"abstract":"<div><p>The aim of this study was to evaluate the total content of flavonoids and phenols, in vitro antioxidant and cytoprotective effects of <i>Sclerocarya birrea</i> fruit exocarp extracts. The fruit exocarp was extracted sequentially in Hexane, Chloroform, Ethyl acetate, and Methanol and the antioxidant properties were evaluated using in vitro antioxidant assays and cytoprotective effects against H₂O₂-induced cell death using HeLa cells. Molecular docking using Autodock 4.2.6 was used to assess the binding interactions of <i>Sclerocarya birrea</i> compounds to the antioxidant enzymes. The total flavonoid and total phenolic contents were in the order Methanol &gt; Ethyl acetate &gt; Chloroform &gt; Hexane (<i>P</i> &lt; 0.05) of <i>Sclerocarya birrea</i> fruit exocarp extracts. The antioxidant properties using DPPH, ABTS, and Ferric reducing antioxidant power, and total antioxidant capacity assays revealed significantly higher (<i>P</i> &lt; 0.05) antioxidant activity in the Methanol &gt; Ethyl acetate &gt; Chloroform &gt; Hexane. Using WST-1 assay, H₂O₂-induced cell loss was effectively reduced by preincubating HeLa cells with Ethyl acetate &gt; Methanol &gt; Chloroform &gt; Hexane (<i>P</i> &lt; 0.05) extracts. Methanol and Ethyl acetate extracts also significantly increased (<i>P</i> &lt; 0.05) the activities of Superoxide dismutase, Catalase and reduced Glutathione. Amongst the identified compounds, Myricetin 3-o-α- rhamsoside (C8) emerged as the best compound to bind to both SOD and CAT with binding energies of − 9.1 and − 8.8 kcal/mol respectively. C8 formed hydrogen bonds and electrostatic interactions with SOD and CAT to act as an agonist. Our findings suggest that Methanol and Ethyl acetate <i>Sclerocarya birrea</i> fruit exocarp extracts improved antioxidant capacity and thus have the potential to be developed into antioxidant products that will alleviate oxidative stress linked diseases.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 2","pages":"325 - 337"},"PeriodicalIF":1.3,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13596-025-00854-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148060779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the hepatoprotective activity of the methanolic extract of Cotinus coggygria","authors":"Shahzad Kamran,&nbsp;Sairah Hafeez Kamran,&nbsp;Mohsin Ahmad Ghauri,&nbsp;Ali Raza,&nbsp;Muhammad Ajaib","doi":"10.1007/s13596-025-00853-7","DOIUrl":"10.1007/s13596-025-00853-7","url":null,"abstract":"<div><p>Plants have long been recognized for their therapeutic potential, with numerous species exhibiting diverse pharmacological properties, including hepatoprotective, antioxidant, and anti-inflammatory effects. <i>Cotinus coggygria</i>, a traditionally used medicinal plant, has demonstrated various biological activities, yet its hepatoprotective potential against drug-induced liver toxicity remains underexplored. This study aimed to evaluate the hepatoprotective effect of the methanolic extract of <i>Cotinus coggygria</i> in paracetamol-induced acute and subacute liver toxicity in rabbits, addressing a critical research gap in exploring natural substances as hepatoprotective agents. Biochemical and histopathological analyses were conducted to assess liver function and tissue integrity. In acute toxicity studies, pretreatment with 250 mg and 500 mg of the plant extract significantly ameliorated (<i>p</i> &lt; 0.05) paracetamol-induced hepatocyte damage, as evidenced by improved biochemical markers and histopathological findings. A lower dose (100 mg and 200 mg) exhibited significant hepatoprotective effects in the subacute hepatotoxicity model. To ensure the safety of prolonged use, in vitro cytotoxicity was evaluated using lower doses of the extract, e.g. 6.25 µM to 800 µM, using normal human hepatic cell lines THLE-3 and IHH, via MTT assay. The results confirmed no evidence of cytotoxic effects on healthy liver cells. These findings highlight the dose-dependent hepatoprotective efficacy of <i>Cotinus coggygria</i> methanolic extract, supported by robust methodological approaches and conclusive biochemical and histological evidence. The study concludes that <i>Cotinus coggygria</i> extract holds significant promise as a natural hepatoprotective agent against paracetamol-induced liver injury, warranting further clinical and molecular investigations to validate its optimized therapeutic potential.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 2","pages":"339 - 348"},"PeriodicalIF":1.3,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148061052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shalaparni’s dual therapeutic potential in rheumatoid arthritis and depression: a bioinformatics study","authors":"Nidhi Joshi,&nbsp;Anupama Modi,&nbsp;Swayamprabha Samantaray,&nbsp;Shrinal Vasa,&nbsp;Shan Shibu,&nbsp;Yashpalsinh Jadeja,&nbsp;Rajeshkumar G. Chaudhari,&nbsp;Trivima Sharma,&nbsp;Bhavin Parekh","doi":"10.1007/s13596-025-00842-w","DOIUrl":"10.1007/s13596-025-00842-w","url":null,"abstract":"<div><p>Rheumatoid arthritis (RA) is a chronic inflammatory disease that is frequently accompanied by depression, suggesting shared inflammatory pathways. This study investigated <i>Desmodium gangeticum</i> (Shalaparni), an Ayurvedic herb containing the anti-inflammatory compound Gangetin, as a potential dual-acting therapeutic agent for RA and depression. We employed an integrative bioinformatics approach to explore the potential of Gangetin, a phytoconstituent of Shalaparni, as a dual-acting therapy. Target genes for RA and depression were retrieved from the DisGeNet database, while predicted molecular targets of Shalaparni were identified using SwissTargetPrediction. BioVenn was then used to visualize and identify overlapping genes among the RA-associated, depression-associated, and Shalaparni-target gene sets. A protein–protein interaction network was constructed using STRING and analyzed using Cytoscape. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were conducted using the DAVID tool. Molecular docking of Gangetin, a phytochemical in Shalaparni, was performed with the top identified proteins using CB Dock. We identified 34 differentially expressed genes shared among RA, depression, and Shalaparni. The PPI network revealed significant interactions with hub genes, such as EGFR, BCL2, GSK3β, PIK3CA, and AKT1. Docking studies showed that Gangetin had high binding affinities with AKT1 and PIK3CA, indicating its potential to target key inflammatory pathways involved in both RA and depression. Shalaparni, through its active compound Gangetin, demonstrated potential as a dual-acting therapeutic agent for RA and depression by targeting shared inflammatory pathways. Further experimental validation is required to explore its clinical application.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"177 - 185"},"PeriodicalIF":1.3,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147341691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dang-Gui-Bu-Xue-Tang triggers GM-CSF and IL-4 secretion corporated with increased amount of Immunoglobulins","authors":"Sheng-Teng Huang,&nbsp;Jia-Hau Yen,&nbsp;Yu-Chun Lin,&nbsp;Ming-Ching Kao,&nbsp;Chia-Ju Chuang,&nbsp;Shu-Tuan Chiang,&nbsp;Te-Mao Li","doi":"10.1007/s13596-025-00851-9","DOIUrl":"10.1007/s13596-025-00851-9","url":null,"abstract":"<div><p>Dang-Gui-Bu-Xue-Tang (DGBXT, 當歸補血湯) is a two-herb decoction that is used in traditional Chinese medicine (TCM) to invigorate the blood. DGBXT reportedly enhances red and white blood cell counts and increases bone regeneration in models. However, the effects of DGBXT on immune responses in humans are unknown. All study participants completed questionnaires measuring quality of life, emotional and cognitive functioning: the Functional Assessment of Chronic Illness Therapy-Fatigue (FACIT Fatigue) Scale; the Pittsburgh Sleep Quality Index (PSQI); and the 36-Item Short Form Health Survey (SF-36). The Milliplex assay evaluated cytokine, chemokine and immunoglobulin expression. DNA microarray hybridization examined gene expression. DGBXT improved levels of physical functioning and pain sensations, according to self-reported health-related quality of life data. Feelings of fatigue and sleep quality were also improved by DGBXT. Moreover, DGBXT enhanced the production of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin 4 (IL-4), interleukin 5 (IL-5) and macrophage inflammatory protein-1β (MIP-1β), and upregulated protein levels of immunoglobulins IgG, IgA and IgM, as well as messenger RNA expression of GM-CSF, IL-5 and IgM. We observed a time-dependent, positive correlation between levels of GM-CSF, IL-4 and MIP-1β and a decline in fatigue among these healthy volunteers. DGBXT supplementation appears to enhance the immune response and improve quality of life.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 2","pages":"349 - 360"},"PeriodicalIF":1.3,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148060998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Thymus fedtschenkoi var. handelii (Ronniger), a natural provider of ursolic and oleanolic acids with significant pharmaceutical value","authors":"Serkan Yigitkan,&nbsp;Ismail Yener,&nbsp;Mehmet Akdeniz,&nbsp;Eda Cavus Kaya,&nbsp;Mustafa Abdullah Yilmaz,&nbsp;Ferhat Celik,&nbsp;Mehmet Fırat,&nbsp;Abdulselam Ertas","doi":"10.1007/s13596-025-00848-4","DOIUrl":"10.1007/s13596-025-00848-4","url":null,"abstract":"<p>The increasing interest in <i>Thymus</i> species is due to their rich phytochemical content and traditional medicinal uses. <i>Thymus fedtschenkoi</i> var. <i>handelii</i>, an endemic and rarely investigated plant, has been studied only for its essential oil. This study aimed to investigate the chemical and biological properties of the essential oil (EO) and ethanol extract (EE) of <i>T. fedtschenkoi</i> var. <i>handelii</i>. The phenolic content of the species was evaluated using LC-MS/MS and GC-MS/FID was used to analyze the chemical structure of triterpenoids, EO and aroma. Moreover, the cytotoxic and antioxidant properties as well as the suppression of tyrosinase, Hmg-CoA reductase, elastase, urease, angiotensin, and collagenase enzymes were investigated. Linalool (33.92%) and thymol (11.02%) in the EO and α-pinene (17.06%) and eucalyptol (16.20%) in the aroma were determined to be high. The species’ root and aerial component EE shown strong antioxidant activity, particularly in the ABTS, DPPH, and CUPRAC techniques. In the enzyme inhibition studies, the aerial parts and root EE showed high urease enzyme activity (80.84 ± 2.31% and 45.01 ± 0.24 at 100 µg/mL, respectively). Based on LC-MS/MS and GC-MS data, it was determined that the root of the species contained notably high concentrations of rosmarinic acid (9308.04 µg analyte/g extract), while the aerial parts contained quinic (5453.32), malic (2326.19), ursolic (145866.04), betulinic (13296.75) and oleanolic (42465.91) acids. Rich in secondary metabolites like linalool, rosmarinic acid, oleanolic acid, and ursolic acid, and exhibiting notable antioxidant and urease enzyme inhibition capacities, the species holds promise as a source of economically valuable compounds for diverse industries.</p>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 1","pages":"251 - 263"},"PeriodicalIF":1.3,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147338658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The aqueous extract of the leaves of Maesa lanceolata reverses pain and inflammation in monosodium urate-induced gouty arthritis in rats","authors":"Antoine Nfundiko,&nbsp;Christian Kuete Fofié,&nbsp;Pius Pum Tseuguem,&nbsp;Roger Hermann Sadie Foguieng,&nbsp;Cédric Wamba Koho,&nbsp;Francis Désiré Tatsinkou Bomba,&nbsp;Baharanyi chasing,&nbsp;Bibiane Aimée Wandji,&nbsp;Télesphore Benoît Nguelefack","doi":"10.1007/s13596-025-00849-3","DOIUrl":"10.1007/s13596-025-00849-3","url":null,"abstract":"<div><p>This study aimed to assess the analgesic and anti-inflammatory effects of the aqueous extract of <i>Maesa lanceolata</i> leaves (AEML) on gouty arthritis induced in rats by the intra-articular injection of 5 mg of monosodium urate (MSU) into the left ankle. Following MSU injection, rats were treated for six days with allopurinol (5 mg/kg/day) or AEML at 45, 90, or 180 mg/kg/day. Ankle and paw diameters, as well as pain sensitivity, were measured daily. After treatment, brain and spinal cord tissues were collected to measure nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD) levels. Cytokine levels (IL-1β and TNF-α) were analyzed in the serum and synovial fluid. Histological analysis of the ankle was conducted, and in vitro studies were performed to assess the anti-inflammatory activity of AEML on macrophages under lipopolysaccharide (LPS) or MSU stimulation. AEML significantly reversed MSU-induced hyperalgesia and reduced ankle and paw swelling. It decreased NO and MDA levels and increased SOD activity in the spinal cord. AEML significantly increased GSH levels in the left-brain hemisphere. Histological analysis revealed reduced inflammation in ankle tissues from AEML-treated animals. However, AEML did not significantly reduce serum or synovial cytokine levels. In vitro, AEML was noncytotoxic and reduced NO production but unexpectedly increased cytokine levels under both normal and inflammatory conditions. AEML alleviated gout-induced pain and inflammation, with effects unrelated to cytokine reduction. These findings suggest alternative anti-inflammatory mechanisms, highlighting AEML as potential therapeutic agent for gouty arthritis.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 2","pages":"361 - 374"},"PeriodicalIF":1.3,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148061097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"METTL3-mediated m6A modification of FoxP4 promotes HCC metastasis","authors":"Guang-zhen Wu,&nbsp;Yi-wei Ren,&nbsp;Yang Liu,&nbsp;Teng Zhao,&nbsp;Zhi-ping Huang,&nbsp;Kai Lu","doi":"10.1007/s13596-025-00850-w","DOIUrl":"10.1007/s13596-025-00850-w","url":null,"abstract":"<div><p>METTL3‐catalyzed m⁶A methylation drives oncogenic processes in HCC. Foxp4 was recently shown to be overexpressed in HCC, linked to poor prognosis. However, the function of METTL3-Foxp4 axis in HCC is still mainly unknown. Utilizing immunohistochemistry (IHC) and quantitative real-time PCR (qRT-PCR), it was possible to determine Foxp4 expression in HCC tissues and cell lines. In order to evaluate the Foxp4 m⁶A level in HCC, MeRIP-PCR was performed. To find the target gene’s “reader” protein, RNA immunoprecipitation (RIP) was performed. We looked at the biological function and probable mechanism of METTL3-Foxp4 axis in HCC in this study. This study showed that METTL3 increased Foxp4 mRNA levels in HCC in a m⁶A dependent manner, while elevated m⁶A-modified FOXP4 correlates with reduced OS and RFS in HCC patients. Subsequently, METTL3 and YTHDF1 regulated the expression of Foxp4 in HCC cells by affecting the mRNA stability of Foxp4. Functional tests showed that METTL3/Foxp4 axis drives HCC metastasis. In addition, in a large cohort study, Foxp4 expression was linked well with METTL3 and YTHDF1 in HCC. In clinic, a better OS was further observed in HCC patients with low level of “writer”-METTL3, “reader”-YTHDF1 and “target” -Foxp4. Our study reveals a mechanism by which m⁶A-RNA regulates oncogene activity, which might result in the creation of a therapeutic strategy for the treatment of HCC.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"26 2","pages":"475 - 482"},"PeriodicalIF":1.3,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148061108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}