杨梅素在白血病细胞中的抗炎潜力:硅学和体外探索

IF 1.8 Q3 PHARMACOLOGY & PHARMACY
Khushbu Nishad, Talambedu Usha, H. P. Prashanth Kumar, Nijalingappa Ramesh, Sushil Kumar Middha
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引用次数: 0

摘要

一种名为 "杨梅素 "的传统植物黄酮类化合物以其广泛的功效而闻名,其中包括强大的抗炎、抗癌和抗氧化特性。在本研究中,我们通过观察白血病癌细胞株(THP-1)中 COX-2、IL-6 和 TNF-α 的表达情况,研究了杨梅素(MN)的抗炎潜力。为了进行体外研究,THP-1 在罗斯威尔公园纪念研究所培养基中培养,培养基中添加了 10%的胎牛血清和青霉素(100 U/ml)。细胞活力和细胞毒性测试采用 MTT 法进行。为制备用于室内研究的配体和蛋白质,从蛋白质数据库中检索了目标抗炎蛋白质 COX-2(PDB ID:5KIR)、TNF-(PDB ID:1TNF)和 IL-6(PDB ID:1ALU)的结构。此外,还使用 AutoDock Vina 软件进行了对接实验。与参考药物顺铂(9.46 微克/毫升)相比,MN 的 IC50 值为 90.161 微克/毫升。与 LPS 诱导的对照细胞相比,用 MN 预处理的细胞在几何平均荧光强度测量下,抗炎成分(COX-2、TNF-α 和 IL6)的表达明显降低。此外,在模拟研究中,MN 与 COX-2、TNF-α 和 IL-6 也有很强的相互作用。总之,MN 在实验室和体外研究中都表现出了强大的抗炎作用。要更全面地了解该分子,还需要进一步的体内研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-inflammatory potential of myricetin in leukemia cells: in silico and in vitro exploration

A conventional plant flavonoid known as myricetin is well-known for its wide range of effects, including its strong anti-inflammatory, anti-cancer, and antioxidant characteristics. In this study, we examined anti-inflammatory potential of Myricetin (MN) by looking at the expressions of COX-2, IL-6 and TNF-α in leukemia cancer cell lines (THP-1). The THP-1 was cultured in Roswell Park Memorial Institute medium supplemented with 10% foetal bovine serum and penicillin (100 U/ml) for the purposes of the in-vitro investigations. Cell viability and cytotoxicity testing were done using the MTT assay. To prepare the ligand and proteins for the in-silico studies, The structures of the targeted anti-inflammatory proteins COX-2 (PDB ID: 5KIR), TNF- (PDB ID: 1TNF), and IL-6 (PDB ID: 1ALU) were retrieved from Protein Data Bank. Furthermore, docking experiments were carried out using the AutoDock Vina software. In comparison to the reference drug cisplatin (9.46 µg/ml) the IC50 value for MN was found to be 90.161 µg/ml. The cells pretreated with MN showed a significantly lower expression of anti-inflammatory components (COX-2, TNF-α and IL6) upon measured by geometric mean fluorescence intensity as compared to the LPS-induced control cells. Additionally, MN has shown a strong interaction with COX-2, TNF-α and IL-6 in simulation studies. To conclude, MN exhibits a potent anti-inflammatory effect both in-silico and in-vitro studies. Further, in vivo investigations are needed to gain more comprehensive understanding of the molecule.

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来源期刊
Advances in Traditional Medicine
Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-
CiteScore
4.30
自引率
0.00%
发文量
50
期刊介绍: Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
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