{"title":"Effects of Crataegus orientalis fruit extract on lipid accumulation in oleic acid-induced HepG2 cells","authors":"Kader Fatma Algül, Turgut Şekerler, Ali Şen, Gizem Gülmez, Azize Şener","doi":"10.1007/s13596-024-00750-5","DOIUrl":null,"url":null,"abstract":"<div><p>During the recent years, much great deal of research has been conducted on potential use of certain herbs and nutraceuticals to manage nonalcoholic fatty liver disease (NAFLD), specifically with those strong biological activities and antioxidant ability. In our research, the goal is to investigation the protective efficacy of the ethanol extract of <i>Crataegus orientalis</i> fruits (COE) aganist lipid accumulation in oleic acid (OA)-induced hepatocellular carcinoma cells (HepG2) at the molecular level. Firstly, antioxidant activity and lipoxygenase activity of the <i>C. orientalis</i> were determined in vitro<i>.</i> For induction of lipid accumulation in HepG2, we established an in vitro model using 1 mM OA. The <i>C. orientalis</i> was evaluated for their ability to prevent from lipid accumulation and effects on sterol regulatory element binding protein-1c (SREBP-1c), acetyl CoA carboxylase (ACC1) and fatty acid synthase (FAS) mRNA levels involved in lipid metabolism. The <i>C. orientalis</i> exhibited moderate antioxidant activity against ABTS and DPPH radicals and a significant anti-inflammatory effect against the 5-lipoxygenase enzyme. The results demonstrated that the COE (100 µg/ml) inhibited lipid accumulation (<i>p</i> < 0.01) and was effective in reducing triglyceride (TG) concentration (<i>p</i> < 0.001) and production of reactive oxygen species (ROS) in HepG2 cells (<i>p</i> < 0.05). <i>C. orientalis</i> suppressed mRNA expression of lipid metabolism enzymes (ACC1, FAS) and transcription factor SREBP-1c (<i>p</i> < 0.001, <i>p</i> < 0.001 and <i>p</i> < 0.01, respectively). Our findings recommend that <i>C. orientalis</i> fruits are potentially protective against lipid accumulation in hepatocytes and may have beneficial effects in the treatment of the NAFLD.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 3","pages":"923 - 933"},"PeriodicalIF":1.8000,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Traditional Medicine","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s13596-024-00750-5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0
Abstract
During the recent years, much great deal of research has been conducted on potential use of certain herbs and nutraceuticals to manage nonalcoholic fatty liver disease (NAFLD), specifically with those strong biological activities and antioxidant ability. In our research, the goal is to investigation the protective efficacy of the ethanol extract of Crataegus orientalis fruits (COE) aganist lipid accumulation in oleic acid (OA)-induced hepatocellular carcinoma cells (HepG2) at the molecular level. Firstly, antioxidant activity and lipoxygenase activity of the C. orientalis were determined in vitro. For induction of lipid accumulation in HepG2, we established an in vitro model using 1 mM OA. The C. orientalis was evaluated for their ability to prevent from lipid accumulation and effects on sterol regulatory element binding protein-1c (SREBP-1c), acetyl CoA carboxylase (ACC1) and fatty acid synthase (FAS) mRNA levels involved in lipid metabolism. The C. orientalis exhibited moderate antioxidant activity against ABTS and DPPH radicals and a significant anti-inflammatory effect against the 5-lipoxygenase enzyme. The results demonstrated that the COE (100 µg/ml) inhibited lipid accumulation (p < 0.01) and was effective in reducing triglyceride (TG) concentration (p < 0.001) and production of reactive oxygen species (ROS) in HepG2 cells (p < 0.05). C. orientalis suppressed mRNA expression of lipid metabolism enzymes (ACC1, FAS) and transcription factor SREBP-1c (p < 0.001, p < 0.001 and p < 0.01, respectively). Our findings recommend that C. orientalis fruits are potentially protective against lipid accumulation in hepatocytes and may have beneficial effects in the treatment of the NAFLD.
期刊介绍：
Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
