Acta pathologica et microbiologica Scandinavica. Section C, Immunology最新文献_第4页

In vitro influence of endotoxin on human mononuclear phagocyte structure and function. 2. Enhancement of the expression of cytoststic and cytolytic activity of normal and lymphokine-activated monocytes. 内毒素对人单核吞噬细胞结构和功能的体外影响。2. 增强正常和淋巴因子活化单核细胞的细胞增殖和细胞溶解活性的表达。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-12-01

J Hammerstrøm

{"title":"In vitro influence of endotoxin on human mononuclear phagocyte structure and function. 2. Enhancement of the expression of cytoststic and cytolytic activity of normal and lymphokine-activated monocytes.","authors":"J Hammerstrøm","doi":"","DOIUrl":"","url":null,"abstract":"The presence of non-toxic concentrations of E. coli endotoxin (LPS) during the in vitro interaction of normal human monocytes and a human tumour cell line (NHIK 3025) enhanced monocyte-mediated target cell cytostasis and cytolysis. Monocyte responsiveness to LPS was greatest at an intermediate stage of in vitro differentiation. The expression of cytostatic and cytolytic activity by human monocytes activated with mediators from Corynebacterium parvum-stimulated human lymphocytes was also enhanced by LPS. Lymphokine activation did not induce additional LPS responsiveness in the monocytes. Monocytes activated with lymphokines and subsequently deactivated by in vitro culture did not show any increase in LPS responsiveness. A soluble cytostatic factor, which is probably not cold thymidine, was released from monocytes exposed first to lymphokines and then to LPS. While LPS is ineffective as an induction signal of monocyte cytotoxicity to tumour cells in this system, it enhances the expression of cytotoxicity induced by prolonged in vitro culture or lymphokine activation.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"87 6","pages":"391-9"},"PeriodicalIF":0.0,"publicationDate":"1979-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11604740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro influence of endotoxin on human mononuclear phagocyte structure and function. 1. Depression of protein synthesis, phagocytosis of Candida albicans and induction of cytostatic activity. 内毒素对人单核吞噬细胞结构和功能的体外影响。1. 抑制蛋白合成，吞噬白色念珠菌，诱导细胞抑制活性。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-12-01

J Hammerstrøm, G Unsgaard

{"title":"In vitro influence of endotoxin on human mononuclear phagocyte structure and function. 1. Depression of protein synthesis, phagocytosis of Candida albicans and induction of cytostatic activity.","authors":"J Hammerstrøm, G Unsgaard","doi":"","DOIUrl":"","url":null,"abstract":"The effect of E. coli endotoxin (LPS) on human monocytes and peritoneal macrophages (PEC) during in vitro differentiation was studied. Short-term (less than 24 hours) exposure to LPS in concentrations up to 50 microgram/ml did not affect monocyte survival or 51Cr-release, but concentrations of 10 microgram/ml or more reduced monocyte survival when LPS exposure was prolonged to 72 h. Undifferentiated monocytes seemed to be sensitive to this effect. Monocyte and PEC protein synthesis was reduced by nontoxic LPS concentrations in both undifferentiated and differentiated cells. LPS exposure reduced monocyte ingestion and degradation of 125I-labelled Candida albicans, dependent on time and dosage. The induction of monocyte- and PEC-mediated cytoststic activity to tumour cells induced by prolonged in vitro culture was also impaired by LPS. The morphological alterations induced in mononuclear phagocytes by LPS included a changes distribution of cells in the monolayer, changes in membrane structure and apparent reduction of lysosomes. LPS thus interferes adversely with several aspects of human mononuclear phagocyte in vitro differentiation.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"87 6","pages":"381-9"},"PeriodicalIF":0.0,"publicationDate":"1979-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11604739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased serum IgE in Hodgkin's disease is of polyclonal origin. 霍奇金病血清IgE升高是多克隆起源。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-12-01

T O Landaas, O Grimmer, H E Heier, T Godal

引用次数: 0

The influence of specific antibodies and cellular immunity in the induction of tolerance of chemotactic activity caused by Bacteroides fragilis lipopolysaccharide. 特异性抗体和细胞免疫在诱导脆弱拟杆菌脂多糖趋化活性耐受中的影响。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-10-01

K Sveen, T Hofstad

{"title":"The influence of specific antibodies and cellular immunity in the induction of tolerance of chemotactic activity caused by Bacteroides fragilis lipopolysaccharide.","authors":"K Sveen, T Hofstad","doi":"","DOIUrl":"","url":null,"abstract":"Exudate aspirated from wound chambers implanted subcutaneously on rabbits at different time intervals after local lipopolysaccharide (LPS) injection, showed a peak of chemotactic mediator concentration in vitro about two hours before the peak of polymorphonuclear lekocytes accumulated in vivo was demonstrated. Injection of LPS locally into the wound chambers three days after the first injection of LPS showed a reduced number of PMNs accumulated in the exudate. Antibodies to the LPS preparation were demonstrated in the exudate and serum by indirect haemagglutination of sheep erythrocytes before the second LPS injection. This antibody titre increased up to two weeks after the first LPS injection, and was slightly higher in the serum than in the exudate. Also, a migration inhibition factor (MIF) activity was demonstrated in the exudates formed. This MIF activity of the exudates increased after the second LPS injection. The increased titre of specific antibody may indicate an accelerated clearance of LPS, and the MIF activity may indicate a reduced response of PMNs to chemotactic mediators. However, the involvement of other biological mechanisms contributing to the decreased response, cannot be excluded.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"87 5","pages":"341-5"},"PeriodicalIF":0.0,"publicationDate":"1979-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11337217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isoelectric focusing of macrophage culture media and the effect of the fractions on the synthesis of DNA and collagen by fibroblasts. 巨噬细胞培养基的等电聚焦及其组分对成纤维细胞合成DNA和胶原的影响。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-10-01

M Jalkanen, J Peltonen, E Kulonen

引用次数: 0

Non-specifically bound IgG and Fc gamma receptors in human malignant tissues. 人恶性组织中非特异性结合的IgG和Fc γ受体。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-10-01

F Wesenberg

引用次数: 0

Cytostatic and phagocytic capacity of lymphokine-activated human monocytes. 淋巴因子激活的人单核细胞的细胞抑制和吞噬能力。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-10-01

G Unsgaard

{"title":"Cytostatic and phagocytic capacity of lymphokine-activated human monocytes.","authors":"G Unsgaard","doi":"","DOIUrl":"","url":null,"abstract":"Human monocytes were rendered cytostatic to the human cell line NHIK 3025 by exposure to lymphokine supernatants (LS) from BCG-stimulated lymphocytes. Exposure to LS for 1, 2 and 4 h induced a considerable cytostatic capacity in the monocytes. However, a stronger cytostatic effect was acquired by exposure to LS for 24 h and 72 h. The phagocytosis of 125I-labelled Candida albicans by LS-activated monocytes was compared with phagocytosis by monocytes treated with control supernatants (CS). The ingestion was increased by short exposure to LS. However, a 72-h exposure to LS induced a decreased ingestion capacity. The capacity of the LS-activated monocytes to digest ingested C. albicans was suppressed. DNA synthesis was increased in the LS-activated monocytes, while protein synthesis was not significantly influenced. The cytostatic capacity of LS-activated monocytes was abolished by culture for 24 h after removal of LS. Following removal of LS or CS with subsequent culture for 4 days, morphological and functional signs of differentiation were less marked in the LS-treated than in the CS-treated cells. The survival was also reduced in the former cells. However, these cells were strongly reactivated by re-exposure to LS.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"87 5","pages":"325-32"},"PeriodicalIF":0.0,"publicationDate":"1979-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11599768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adhesion and locomotion of human leukocytes in vitro; importance of protein coating; effect of lidocain, ethanol and endotoxin. 人白细胞在体外的粘附和运动;蛋白质包衣的重要性;利多卡因、乙醇及内毒素的作用。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-10-01

A Schreiner, G Hopen

引用次数: 0

Autoantibodies in serum and sputum from patients with cystic fibrosis. 囊性纤维化患者血清和痰中的自身抗体。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-10-01

P O Schiøtz, E M Egeskjold, N Høiby, H Permin

{"title":"Autoantibodies in serum and sputum from patients with cystic fibrosis.","authors":"P O Schiøtz, E M Egeskjold, N Høiby, H Permin","doi":"","DOIUrl":"","url":null,"abstract":"Sera from 89 patients with cystic fibrosis (CF) and 88 control persons were examined for the occurrence of rheumatoid factors (RF) of the IgG, IgA and IgM classes by an indirect immunofluorescence method and by the latex fixation slide test. The prevalence of RF-IgG was significantly higher (88%) (p less than 0.0005) among the CF patients than among the control persons (7%), while no difference was found between the two groups with regard to RF of the IgA or IgM classes. Fifty-five of the CF patients had chronic Pseudomonas aeruginosa infection in their lungs and two or more precipitins against these bacteria in their sera determined by crossed immunoelectrophoresis. These CF patients did not differ from the 34 CF patients without chronic P. aeruginosa infection, neither with regard to prevalence nor titer of RFs, but there was a positive correlation between the number of P. aeruginosa precipitins in the 55 chronically infected CF patients and their titers of IgG-RF. Nineteen CF patients were examined also for RFs, antinuclear antibodies (ANA) and anti-DNA antibodies in their sputum sol phase and corresponding sera. RFs were demonstrated in the sputum sol phase from 6 of the patients by the latex fixation test, whereas their sera were negative in this test, possibly indicating a local production of RF. Positive reactions for ANA and anti-DNA antibodies were found in 7 and 10 of the sputa respectively, and in higher titers than in the corresponding sera, also suggesting a local production. Titers of autoantibodies in sputum were low and no difference was found between patients with chronic P. aeruginosa infection and patients without P. aeruginosa infection. The possible role of autoantibodies in the patogenesis of pulmonary tissue damage in CF patients is discussed.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"87 5","pages":"319-24"},"PeriodicalIF":0.0,"publicationDate":"1979-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11337338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical demonstration of intracellular immunoglobulin in formalin fixed, paraffin embedded sections, as staining method in diagnostic work. 免疫组织化学显示细胞内免疫球蛋白在福尔马林固定，石蜡包埋切片，作为染色方法在诊断工作。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-08-01

P P Clausen, M Jacobsen, P Johansen, N Thommesen

引用次数: 0