Acta pathologica et microbiologica Scandinavica. Section C, Immunology最新文献_第3页

A "reverse" solid-phase radio-immuno-assay for IgM-antibodies to hepatitis A virus. A型肝炎病毒igm抗体的“反向”固相放射免疫测定。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1981-04-01

O H Meurman, L Matter, R V Krishna, U H Krech

{"title":"A \"reverse\" solid-phase radio-immuno-assay for IgM-antibodies to hepatitis A virus.","authors":"O H Meurman, L Matter, R V Krishna, U H Krech","doi":"","DOIUrl":"","url":null,"abstract":"A \"reverse\"solid-phase radio-immuno-assay for IgM antibodies to hepatitis A virus (HAV) was developed. Anti-human IgM immunoglobulins were bound on the wells of polyvinylchloride microtiter plates. Serum specimens were incubated in the anti-human IgM coated wells and bound IgM antibodies were then assayed for antigen specificity by subsequent incubations with HAV antigen and 125I-labelled human anti-HAV IgG. The test showed a high sensitivity and specificity for anti-HAV IgM antibodies. No false-positive reactions were observed either in the sera from patients with hepatobiliary disorders other than HAV infection or in the sera containing both rheumatoid factor and anti-HAV IgG antibodies. In acute HAV infections specific IgM antibodies were present already in the first specimens taken within a few days after the onset of jaundice. The persistence of the IgM antibodies was from 4 to 6 months. IgM antibody titers up to 1,000,000 were observed in the acute phase of HAV infection. In routine diagnostic work the titration of the sea was not necessary, since a reliable qualitative result was obtained by testing the sera in a single dilution of 1:100. A similar \"reverse\" immuno-assay principle may be adaptable for the diagnostic determination of IgM antibodies to different viral and microbial antigens.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"89 2","pages":"79-84"},"PeriodicalIF":0.0,"publicationDate":"1981-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17333627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serotype determinant protein of Neisseria Meningitidis. Large scale preparation by direct detergent treatment of the bacterial cells. 脑膜炎奈瑟菌血清型决定蛋白。用直接去污剂处理细菌细胞的大规模制备。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1981-04-01

T B Helting, G Guthöhrlein, F Blackkolb, H Ronneberger

{"title":"Serotype determinant protein of Neisseria Meningitidis. Large scale preparation by direct detergent treatment of the bacterial cells.","authors":"T B Helting, G Guthöhrlein, F Blackkolb, H Ronneberger","doi":"","DOIUrl":"","url":null,"abstract":"Neisseria meningitidis Group B microorganisms, inactivated with phenol and harvested by centrifugation, were subjected to direct treatment with various detergents to solubilize the serotype determinant proteins localized in the outer membrane. Analysis of the data showed that extraction of the cells with detergents provided yields of the serotype protein substantially exceeding those obtained by simple salt extraction of the bacteria. Routinely, more than 2 mg of end product per g of cell mass (wet weight) may be recovered by the present method. By gel chromatographic analysis, the serotype determinant protein was shown to interact with the capsular polysaccharides derived from Group A or C Neisseria meningitidis microorganisms, forming high molecular weight complexes. This interaction markedly enhanced the solubility of the serotype determinant protein. Combined vaccines of the type-specific protein with the group- specific polysaccharides were evaluated for their immunogenic potential in the subcutaneous steel spring implant model. In guinea pigs, amounts corresponding to 10 micrograms completely prevented infection upon challenge with homologous organisms four weeks after immunization. Partial protection was observed with immunizing doses corresponding to 2 micrograms or 0.4 micrograms/animal, respectively. Compared to lyophilized preparations, vaccines adsorbed to a mineral carrier were slightly less effective in inducing protection, whereas inclusion of Bordetella pertussis as a component of the vaccine stimulated the immune response.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"89 2","pages":"69-78"},"PeriodicalIF":0.0,"publicationDate":"1981-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17845881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental Escherichia coli 06 infection in mice. III. Effects of malnutrition, immunization and nutritional restoration. 实验性小鼠大肠杆菌06感染。3营养不良、免疫和营养恢复的影响。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1981-02-01

S Ahlstedt

引用次数: 0

Experimental Escherichia coli 06 infection in mice. IV. On the relative importance of complement factors and antibodies for the host defence. 实验性小鼠大肠杆菌06感染。关于补体因子和抗体对宿主防御的相对重要性。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1981-02-01

S Ahlstedt

{"title":"Experimental Escherichia coli 06 infection in mice. IV. On the relative importance of complement factors and antibodies for the host defence.","authors":"S Ahlstedt","doi":"","DOIUrl":"","url":null,"abstract":"Treatment of CBA mice with cobra venom factor (CVF) decreased their C3 levels to less than 10 per cent of normal and resulted in a simultaneous increase of their susceptibility to intraperitoneal E. coli 06 infection. Animals immunized and treated with CVF had an infection resistance similar to untreated controls but considerably less than mice immunized only. The CVF treatment did not affect the antibody formation after immunization. Untreated or immunized AKR mice deficient in C5 did not show decreased resistance to the infection. Exposure of NMRI mice to preformed antigen-antibody complexes unrelated to the infection did not increase the susceptibility of the animals to infection, neither did it impair their C3 levels. Two out of five freshly frozen (-70 degrees C) normal rabbit sera gave significant, heat-labile protection of mice to the E. coli infection without containing any antibodies detectable with the ELISA. This protective capacity was not abolished with zymosan treatment affecting the levels of complement factors and could be \"natural antibodies\" stimulated from the environment. In testing whether similar protective factors as those found in normal rabbit serum could be raised in CBA mice, such animals were orally given killed E. coli 06 bacteria. This did not affect the resistance to the E. coli infection or to the rise of specific antibodies after immunization.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"89 1","pages":"23-8"},"PeriodicalIF":0.0,"publicationDate":"1981-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18076091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hereditary neurologic disorders, characterized by ataxia. Immunological in vitro parameters and HLA. 遗传性神经系统疾病，以共济失调为特征。体外免疫参数与HLA。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1980-12-01

L Pedersen, P Platz, N E Raun

引用次数: 0

Leukocyte migration in different systems. Effect of colchicin. Kinetics of migration under agarose. 白细胞在不同系统中的迁移。秋水仙素的作用。琼脂糖作用下的迁移动力学。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1980-04-01

A Schreiner, T Kalager, D Vaula

引用次数: 0

Experimental Escherichia coli 06 infection in mice. 1. Effect of immunisation on resistance in relation to 06 antibody levels in mice of different strains. 实验性小鼠大肠杆菌06感染。1. 免疫对不同品系小鼠抗06抗体水平的影响。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1980-02-01

S Ahlstedt

{"title":"Experimental Escherichia coli 06 infection in mice. 1. Effect of immunisation on resistance in relation to 06 antibody levels in mice of different strains.","authors":"S Ahlstedt","doi":"","DOIUrl":"","url":null,"abstract":"The susceptibility to intraperitoneal infection with E. coli 06:k2 bacteria and the increase of resistance after immunization and immune serum injection was analysed in eight mouse strains (CBA, A/J, C3H, C3H/HeJ, C57 B1/6J congenitally athymic C57 B1 nu/nu and their heterozygous nu/+ litter mates as well as NMRI mice). A different susceptibility to the infection was found among the strains. This was not related to endotoxin resistance or thymus deficiency or to the ability of the animals to form antibodies as measured with the enzyme-linked immunosorbent assay, ELISA. Immunization of the animals with 5 x 10(7) E. coli 06:K13 bacteria resulted in a smaller increase in resistance in the less susceptible CBA mice than in the more susceptible A/J or C3H/HeJ mice. This pattern was further accentuated after repeated immunization. The development of resistance by immunization seemed independent of T-cells, since the nu/nu mice were as resistant as the nu/+ mice. The nu/nu mice, however, formed less antibodies after vaccination than did their nu/+ litter mates. The lowest antibody responses were noted in the NMRI mice, but this was accompanied with similar increase in resistance compared with the other strains forming 10-fold more 06 antibodies. Immunization with as little as 102-104 bacteria also resulted in a rise in resistance. This was, however, accompanied by a minute increase in antibody titer. Despite content of minute antibody levels administration of such immune serum gave protection of the recipients. It was concluded that very small amounts of antibodies would provide protection against E. coli infection varying from one mouse strain to another.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"88 1","pages":"39-45"},"PeriodicalIF":0.0,"publicationDate":"1980-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18039694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of the bactericidal capacity of blood granulocytes. Evaluation of a new method and the effect of levamisole. 活化血液中粒细胞的杀菌能力。左旋咪唑新方法及效果评价。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-12-01

J Palmblad, L Engstedt

引用次数: 0

Anticomplementary activity in diffuse and nodular goiters. 弥漫性和结节性甲状腺肿的抗补体活性。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-12-01

U Feldt-Rasmussen, K Bech, J Date, S Thyme, J Hannover Larsen, H Nielsen

{"title":"Anticomplementary activity in diffuse and nodular goiters.","authors":"U Feldt-Rasmussen, K Bech, J Date, S Thyme, J Hannover Larsen, H Nielsen","doi":"","DOIUrl":"","url":null,"abstract":"The occurrence of anticomplementary activity and its correlation to serum thyroglobulin was investigated in 71 patients with thyroid diseases and 63 age and sex matched control subjects. The patients which were subgrouped according to thyroid function and characteristics of the goiter, were examined at the time of diagnosis. The anticomplementary activity was measured by a complement consumption (CC) assay. Sera from patients with Graves' disease and nontoxic diffuse goiter, showed stronger activity than sera from patients with nontoxic goiter. Seventeen of the patients and one of the controls were positive in the CC-assay. The percentage hemoglobin release in this assay was normally distributed using control sera but not for the patient group. There was no correlation either between CC-activity and serum concentrations of thyroglobulin or CC-activity and antibodies to the O-antigen of Yersinia enterocolitica serotype 3, thyroid cytoplasma, thyroglobulin, nuclear factors, streptolysin O, streptococ hyaluronidase, and parietal cells, respectively. However, correlation between the levels of thyroid stimulating immunoglobulins and CC-activity, was noted (Rho = 0.511, P less than 0.05), which suggests that these immunoglobulins also are present as immune complexes. Thyroglobulin--antithyroglobulin complexes preformed in vitro at high thyroglobulin concentration, gave negative results in the CC-assay.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"87 6","pages":"365-70"},"PeriodicalIF":0.0,"publicationDate":"1979-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11785834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Donor-specific cell-mediated cytotoxicity in renal allograft recipients. 同种异体肾移植受者供体特异性细胞介导的细胞毒性。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-12-01

E Dickmeiss

{"title":"Donor-specific cell-mediated cytotoxicity in renal allograft recipients.","authors":"E Dickmeiss","doi":"","DOIUrl":"","url":null,"abstract":"The immunological in vitro responses of cadaver kidney transplanted patients against donor specific target cells were investigated with the test systems for antibody-dependent cell-mediated cytotoxicity (ADCC) and direct cell-mediated cytotoxicity (DCMC). The test systems were used in 58 recipients just before the transplantation. Nine recipients (17%) had positive donor directed ADCC reactivity and of these seven had early rejection episodes, but this outcome was not significantly different from the outcome in the ADCC negative group. Only one recipient showed positive donor directed DCMC reactivity and had early loss of the graft in rejection. The test systems were further used to monitor the donor specific responces after the transplantation in 32 recipients. Positive DCMC reactivities were significantly correlated with clinical rejections but most often positive reactions occurred too late to be of predictive value. The donor specific ADCC reactivity after transplantation was not clearly correlated with rejections. It is concluded that the predictive and diagnostic values of the two test systems were too weak to justify their use as routine methods in the clinical management of the kidney transplanted patient.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"87 6","pages":"371-76"},"PeriodicalIF":0.0,"publicationDate":"1979-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11606179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0