American journal of dentistry最新文献

{"title":"Evaluation of tooth color, whiteness changes and sensitivity following laser-assisted in-office tooth bleaching using Er,Cr:YSGG laser: A prospective clinical study.","authors":"Athina Tsaltampasi, Dimitrios Dionysopoulos, Dimitrios Strakas, Constantinos Papadopoulos, Effimia Koumpia, Pantelis Kouros, Moschos Papadopoulos, Kosmas Tolidis","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>This prospective clinical study evaluated the tooth color (ΔΕ*ab and ΔΕ₀₀) and whiteness (ΔWID) changes, as well as tooth sensitivity (TS) induced after performing in-office tooth bleaching with or without light-activation of the bleaching gel using Er,Cr:YSGG laser irradiation.</p><p><strong>Methods: </strong>20 subjects were enrolled for the study. The bleaching session was conducted with two 20-minute sessions of a 40% hydrogen peroxide bleaching agent assisted by Er,Cr:YSGG laser (2,780 nm) at the left hemiarch and without laser activation on the right hemiarch. ΔΕ*ab, ΔΕ₀₀ and ΔWID were evaluated in central incisors and canines using a clinical spectrophotometer immediately, 7 days and 30 days after the bleaching procedure. TS was evaluated during the bleaching treatments in both hemiarches of the patients using a visual analogue scale.</p><p><strong>Results: </strong>The results revealed a significant increase in tooth color and whiteness changes following the tooth bleaching in both laser-treated and conventional groups and in all teeth. Tooth color and whiteness changes were observed immediately after the different treatments and were gradually reduced after 7 and 30 days (P< 0.05). Between the conventional and laser-assisted technique, there were statistically significant differences only in canines (P< 0.05) and in lower central incisors (P< 0.05). Mild TS was noticed in 85% of the patients, without differences between the bleaching treatments (P> 0.05).</p><p><strong>Clinical significance: </strong>Er,Cr:YSGG laser-assisted tooth bleaching treatment may enhance tooth color and whiteness changes but not in all the examined tooth types, without inducing more intense tooth sensitivity compared to the conventional technique.</p>","PeriodicalId":7538,"journal":{"name":"American journal of dentistry","volume":"38 2","pages":"71-78"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of immediate dentin sealing and temporary cement removal methods on microtensile bond strength of a universal resin cement.","authors":"Oğulcan Yücel, Muharrem Erhan Çömlekoğlu, Mine Dündar Çömlekoülu, Ege Çolak, Makbule Heval Şahan","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the impact of different surface cleaning methods and immediate dentin sealing (IDS) on the bond strength of a universal adhesive resin cement to dentin, using a 3D-printed restorative material.</p><p><strong>Methods: </strong>Extracted human third molars were prepared and divided into two groups: with and without IDS. Temporary restorations were cemented onto the prepared tooth surfaces. Five surface cleaning methods were applied to both groups: pumice, pumice with soap, sandblasting, EDTA, and laser. 3D printed resin blocks were fabricated and bonded to the teeth using a universal adhesive resin cement. After thermal cycling and water storage, microtensile bond strength tests were conducted. Failure modes were analyzed using light microscopy and SEM. Statistical analyses were made.</p><p><strong>Results: </strong>Microtensile bond strength (µTBS) was significantly influenced by both surface cleaning methods and IDS application. Sandblasting and laser cleaning resulted in the highest µTBS values, particularly when combined with IDS. IDS significantly increased µTBS compared to the control group, regardless of the cleaning method. SEM analysis revealed that adhesive failure was the predominant failure mode in most groups, with some mixed failures observed.</p><p><strong>Clinical significance: </strong>Prepared tooth surface cleaning procedures increased the microtensile bond strength of the universal adhesive resin cement regardless of immediate dentin sealing.</p>","PeriodicalId":7538,"journal":{"name":"American journal of dentistry","volume":"38 2","pages":"79-86"},"PeriodicalIF":0.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bleaching of stained teeth using Cu-coupled TiO₂ nanoparticles using hydrogen peroxide and blue light.","authors":"Byeong Jin Gu, Sungae Son, Jeong-Kil Park, Yong Hoon Kwon, Franklin Garcia-Godoy","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the efficacy of copper-doped titanium dioxide (Cu-TiO₂) nanoparticles (NPs) in bleaching the stained teeth when subjected to hydrogen peroxide (H₂O₂) and blue light irradiation.</p><p><strong>Methods: </strong>Cu-TiO₂ NPs were synthesized by mixing TiO₂ and Cu, followed by calcination. Their light absorbance was measured. Photocatalytic reactions were evaluated using methylene blue (MB), 3,3',5,5'-tetramethylbenzidine (TMB), and terephthalic acid (TA) assays. Color changes in stained teeth were monitored under various conditions, including the presence or absence of 3% H₂O₂ and light irradiation. Additionally, the cytotoxicity of Cu-TiO₂ NPs on normal oral cells (periodontal ligament fibroblast: PDLF) was evaluated.</p><p><strong>Results: </strong>Unlike pristine TiO₂, Cu-TiO₂ exhibited a slightly increased absorbance in the 600-800 nm range due to the incorporated Cu. Cu-TiO₂ demonstrated high MB degradation and a marginal increase in peak intensity at 650 nm upon combined H₂O₂ and light irradiation. The TA assay showed a gradual increase in peak fluorescence intensity near 425 nm with increasing light exposure. Stained teeth treated concurrently with Cu-TiO₂ NPs, 3% H₂O₂, and laser irradiation exhibited significantly superior color changes. Importantly, the tested Cu-TiO₂ NPs displayed negligible toxicity to PDLF at concentrations up to 500 ppm.</p><p><strong>Clinical significance: </strong>Cu-TiO₂ NPs, when utilized with 3% H₂O₂ and blue laser, offer enhanced bleaching efficacy through photoinduced radical generation compared to 15% H₂O₂ alone, while maintaining negligible damage to normal oral cells at concentrations of 500 ppm or higher.</p>","PeriodicalId":7538,"journal":{"name":"American journal of dentistry","volume":"39 2","pages":"101-104"},"PeriodicalIF":1.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical effects of stannous fluoride dentifrice on peri-implant mucositis, plaque microbiome, and oxidative stress.","authors":"Malgorzata Klukowska, Niranjan Ramji, Alejandra Muñoz Bodnar, Ping Hu, Hao Ye, Sancai Xie, Lijuan Li, Julie Ashe, Tim Reichling, Jiazhen Wang, Kimberly Milleman, Jeffery Milleman","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>This single-center, single-treatment, 4-week study evaluated the efficacy of a stannous fluoride (SnF₂) dentifrice in reducing peri-implant mucositis while assessing changes in biomarkers and the oral microbiome profile.</p><p><strong>Methods: </strong>24 healthy participants 18 years of age or older with osseointegrated implants were included, with 19 participants having mucositis and five without mucositis. The non-mucositis participants served as a reference group for microbiome and biomarker assessments (baseline comparison). All participants used the same 0.454% SnF₂ dentifrice (Crest Pro-Health Sensitive and Enamel Shield) and a soft manual toothbrush twice daily throughout the study. Participants received Modified Gingival Index (MGI) and Gingival Bleeding Index (GBI) assessments at Baseline - Day 1 and at Week 4 - Day 1. Subgingival plaque and peri-implant crevicular fluid (PICF) were collected on Baseline - Day 2 and Week 4 - Day 2 for oxidative stress markers, bacterial endotoxins, proinflammatory cytokines and 16S analysis.</p><p><strong>Results: </strong>After 4 weeks, participants with mucositis experienced a significant reduction from baseline in MGI and GBI scores (63.4% and 79.3%, respectively) and in the number of bleeding sites (72.5% based on GBI analysis). Microbiome analysis of subgingival plaque showed that mucositis was associated with a higher relative abundance of disease-associated genera (Fusobacterium, Porphyromonas, Treponema, and Prevotella) and a lower relative abundance of commensal genera (Rothia and Actinomyces). Alpha diversity was higher in the mucositis group compared to the non-mucositis group at baseline. By Week 4, the profile of participants with mucositis had shifted to align more closely with that of non-mucositis participants. Participants with mucositis showed significant reduction in biomarkers related to bacterial insult, plaque virulence, oxidative stress, and inflammation. Simulated pathway and process analysis revealed that multiple categories of genes were associated with a state of mucositis, and 4 weeks of use of the experimental dentifrice downregulated several virulence-associated genes.</p><p><strong>Clinical significance: </strong>In participants with mucositis, use of a SnF₂ dentifrice for 4 weeks reduced clinical signs and key biomarkers of peri-implant inflammation and shifted the oral microbiome toward a healthier profile, highlighting the utility of SnF₂ dentifrice in the control of peri-implant mucositis.</p>","PeriodicalId":7538,"journal":{"name":"American journal of dentistry","volume":"38 2","pages":"59-66"},"PeriodicalIF":1.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of calculus removal with hand and ultrasonic instruments on titanium surfaces.","authors":"Maggie E Eslinger, Vrushali Abhyankar, Franklin Garcia-Godoy, Brian R Morrow, Pooja Ajitsankardas","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the impact of different instruments on dental implants on titanium discs and simultaneously test how effective they were in removing calculus.</p><p><strong>Methods: </strong>Artificial calculus was applied on 36 rectangular areas demarcated on titanium discs. Surface roughness was measured using a stylus profilometer. Calculus was then removed using six different hand or power-driven ultrasonic instruments. Surface roughness measurements D-Ra and D-Rz were then re-calculated. Cleaned distance was divided by total calculus to yield a cleaning ratio and the number of cleaning strokes required to remove the calculus was calculated. All measurements were statistically analyzed independently (two-way ANOVA, Holm-Sidak roughness and cleaning ratios Kruskal-Wallis one-way ANOVA on ranks, and Student-Newman-Keuls, α< 0.05).</p><p><strong>Results: </strong>Ultrasonic magnetostrictive instruments were most efficient in calculus removal and demonstrated a statistically high cleaning ratio percentage (P< 0.001) and required the least number of strokes compared to all the other groups. The resin curettes were the least effective in calculus removal and required the greatest number of strokes. When surface roughness values (D-Ra) were compared between different instruments, statistically significant differences were noted between the resin curettes and ultrasonic magnetostrictive groups, with the resin curette group showing lower values and the Ultrasonic magnetostrictive group showing greater values.</p><p><strong>Clinical significance: </strong>Current research on significance of titanium particles and their possible role in causing periimplantitis emphasizes the importance of using instruments that do not damage the implant surface. Instrumentation causing the least amount of surface roughness should be considered since the risk of damaging the surface outweighs the benefit.</p>","PeriodicalId":7538,"journal":{"name":"American journal of dentistry","volume":"38 1","pages":"22-26"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bond strength evaluation of universal adhesives applied in etch-and-rinse mode on sound and irradiated dentin.","authors":"Anh D Nguyen, Sina Broscheit, Dirk Vordermark, Kerstin Bitter, Reinhard Gerlach, Christian R Gernhardt","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the laboratory microtensile bond strength (µTBS) of universal adhesives applied in etch-and-rinse mode on irradiated and sound human dentin.</p><p><strong>Methods: </strong>Dentin specimens of 135 freshly extracted human third molars were divided in three superordinate groups. Each tooth received either no irradiation (control group), 5 Gy (2 Gy/2 Gy/1 Gy in 3 consecutive days) or the high-level therapeutic dose of 60 Gy (test groups) applied fractionally (2 Gy/day, 5 days/week, 6 weeks). Samples from each group were randomly divided into three subgroups assigned to three established universal adhesives (Futurabond U, AdheSE Universal, Xeno Select) in just the etch-and-rinse application mode (n= 15). Bond strength was assessed with a universal testing machine 15 minutes after application and light curing of the adhesive materials. Statistical analysis was performed using a two-way ANOVA (P< 0.01) and Tukey's test (P< 0.05).</p><p><strong>Results: </strong>Regarding all experimental groups ANOVA showed significant effects on µTBS of dentin irradiation and the used adhesives (P< 0.01, ANOVA). In nearly all groups, a decrease of µTBS was observed when used on highly irradiated dentin. However, comparing the different groups, no significant influence of high-level radiation on the microtensile bond strength of the three universal adhesives assessed in etch-and-rinse application mode could be determined (P> 0.05, Tukey's test).</p><p><strong>Clinical significance: </strong>Within the limitations of this laboratory study, no significant effects of low- and high-level tumor irradiation on the bond strength of universal adhesives can be detected after application in etch-and-rinse mode. Consequently, this mode of application may be considered a viable option for patients who have undergone radiation therapy when using universal adhesive systems for luting restorative materials.</p>","PeriodicalId":7538,"journal":{"name":"American journal of dentistry","volume":"38 1","pages":"15-21"},"PeriodicalIF":1.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of infection control barriers of light curing units on adhesive cementation of glass ceramics to enamel.","authors":"Begüm Ünlü Kurşun, Ayşe Atay, Pinar Mavigözlü, Ebru Karabece Cal","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the effect of different infection control barriers used with light curing units (LCU) on the shear bond strength (SBS) of glass ceramics with various translucencies and thicknesses during adhesive cementation.</p><p><strong>Methods: </strong>60 extracted molar teeth cut in halves mesio-distally to obtain 120 enamel surfaces. Lithium disilicate blocks (GC, Initial Lisi Blocks); 30 High Translucency (HT) 0.5 mm, 30 Low Translucency (LT) 0.5 mm, 30 HT 1.0 mm, 30 LT 1.0 mm discs with 3 mm diameter were produced (n=120). Samples were categorized into three groups according to the infection control barriers (n=40): 1-LCU without barrier, 2-Disposable barrier on LCU (Pinnacle Cure Sleeve), 3-LCU wrapped with stretch film. The SBS test was performed after thermocycling. Failure modes were determined at x20 and classified and statistically analyzed (P< 0.05).</p><p><strong>Results: </strong>Differences between all the groups were significant (P< 0.001). The highest SBS values were obtained as HT LCU 0.5 mm, LT LCU 0.5 mm, HT Sleeve 0.5 mm and LT Sleeve 0.5 mm, respectively. Highest SBS values were seen in HT LCU 0.5 mm (16.51± 0.68), while LT 1.0 mm stretch (10.76± 1.37) showed the lowest values. Mostly mixed failures occurred in the 0.5 mm sample groups showing higher SBS values.</p><p><strong>Clinical significance: </strong>While the application of a sleeve on a LCU may slightly reduce the shear bond strength values of glass ceramics, it can still be beneficial for the cementation of these materials as it helps prevent cross-infection.</p>","PeriodicalId":7538,"journal":{"name":"American journal of dentistry","volume":"39 1","pages":"47-52"},"PeriodicalIF":1.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of bisphosphonates on periodontal diseases in menopausal and postmenopausal women: A systematic review and meta-analysis.","authors":"Linlin Qin, Xinyue Wu, Kunshuang Wu, Hanyue Mai, Ping Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To systematically evaluate the effect of bisphosphonates on periodontal diseases in menopausal and postmenopausal women.</p><p><strong>Methods: </strong>Databases (PubMed, Embase, Web of Science, Cochrane Library databases, Chinese Scientific and Technological Journal database, Wan Fang Data, China Biomedical Literature Database, and Chinese National Knowledge Infrastructure) were searched from inception to July 2024, languages are Chinese and English. Randomized controlled trials (RCTs) reporting the effect of bisphosphonates in menopausal and postmenopausal women with periodontitis were included. The risk of bias was performed using the Cochrane collaboration tool. The primary outcome was clinical attachment loss (CAL), and the secondary outcomes were probing depth (PD) and gingival index (GI). The analysis of the data was performed using Rev Man 5.3 and Stata 16.0.</p><p><strong>Results: </strong>The meta-analysis incorporated four studies that fulfilled the inclusion criteria. In evaluating the efficacy of bisphosphonates against control treatments, there was high heterogeneity observed in CAL (P = 0.0002; I² = 85%) and PD (P< 0.00001; I² = 93%) within the study groups. Meta-analysis showed a significant improvement in CAL gain (MD = - 0.57 mm; 95% CI = -1.04 to -0.11 mm; P< 0.05), PD reduction (MD = - 0.50 mm; 95% CI = -0.96 to -0.05 mm; P< 0.05), and GI reduction (MD = -1.11; 95% CI = -1.22 to -1.01; P< 0.00001) for bisphosphonate treatment vs. bisphosphonate-naïve therapy.</p><p><strong>Clinical significance: </strong>Bisphosphonate treatment seems to be beneficial for managing periodontitis in menopausal and postmenopausal women.</p>","PeriodicalId":7538,"journal":{"name":"American journal of dentistry","volume":"38 1","pages":"33-38"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of remineralization product on the microhardness and surface roughness of enamel after bleaching agents.","authors":"Dilan Kopuz, Özlem Erçin, Dilek Tağtekin, Funda Yanikoğlu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the effect of toothpaste containing sodium fluoride, a commercially available remineralization product, on the microhardness and roughness of enamel after whitening with two different concentrations of hydrogen peroxide.</p><p><strong>Methods: </strong>32 premolar teeth extracted for orthodontic reasons were collected. Initial microhardness and roughness values were measured. All samples were immersed in a coffee solution for 1 week to simulate coloration in the oral environment and ensure standardization. Then, the second microhardness and roughness measurements were obtained. Two samples were separated, and the remaining ones were divided into three groups; Group 1: Group 2: Samples treated with Beka HP 30% bleaching agent; Group 3: Samples kept in distilled water. For Groups 1 and 2, the bleaching procedure was applied in three sessions of 15 minutes each, following the manufacturer's instructions. Afterward, measurements of all samples were repeated. To examine the reversibility of surface changes following the bleaching process, a demineralization-remineralization (pH) cycle was applied. Subsequently, microhardness and roughness measurements were repeated, and two samples from each group were set aside for FE-SEM Field emission scanning electron microscope analysis. A total of eight samples underwent FE-SEM analysis at x1,000 and x5,000 magnifications. Data were analyzed using two-way ANOVA and Kruskal-Wallis tests.</p><p><strong>Results: </strong>Initial microhardness values of all samples were the highest values, with a decrease observed after the bleaching procedure. Even though the microhardness of the samples increased in Groups 1 and 2 after the pH cycle, there was no significant difference (P< 0.05). While the roughness values of the samples decreased after the discoloration (P> 0.05), they increased after the pH cycle and there were significant differences between the 4th measurement and the other measurements (P< 0.05).</p><p><strong>Clinical significance: </strong>The bleaching agent causes changes in the structure of the enamel and reduces microhardness and roughness values. However, remineralizing agents applied during the pH cycle may have a positive effect on microhardness.</p>","PeriodicalId":7538,"journal":{"name":"American journal of dentistry","volume":"38 1","pages":"9-14"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of amino acid-based carbon dots: Effect on oral cancer cells.","authors":"So-Young Park, Wooil Kim, Ae-Ri Pyo, Yong H Kwon, Franklin Garcia-Godoy","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To assess if amino acid-based carbon dots (CDs) eliminate in vitro cancer cells.</p><p><strong>Methods: </strong>CDs were synthesized using cysteine as a precursor and metal and photosensitizer as modifiers. In vitro cell viability tests were performed using cancer and normal cells without and with H₂O₂ and laser irradiation. Methylene blue (MB) solution was used to evaluate the production of ROS by CDs via observing absorbance differences before and after treatments. Extracellular oxygen production was measured in real-time using a probe. The generation of singlet oxygen was spectroscopically evaluated using RNO-ID assay.</p><p><strong>Results: </strong>Within the test conditions, CDs fn-Cei-Ce6 achieved significantly low in vitro cancer cell viability (near to 26% of initial cell count) at 20 ppm concentration, high absorbance difference (Δ) in all wavelengths, oxygen production approximately to 1.2 mg/L, and high absorbance decrease of RNO-ID assay solution all with 100 µM H₂O₂ and laser irradiation conditions.</p><p><strong>Clinical significance: </strong>Amino acid-based carbon dots (CDs) eliminated cancer cells in vitro with near to 74% of initial cancer cells under 100 µM H₂O₂ and laser irradiation conditions. The results may be extended to the clinical potential of fn-Cei-Ce6 in treating tumors in the oral cavity.</p>","PeriodicalId":7538,"journal":{"name":"American journal of dentistry","volume":"38 1","pages":"46-50"},"PeriodicalIF":0.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}