AMB Express最新文献

{"title":"Engineered Prx-LCA2 fusion protein restores oxidative skin damage via enhanced intracellular peroxidase delivery.","authors":"Di Liu, Yafen Zhan, Jiaqi Qu, Hongping Qiao, Na Li, Yeli Zhang, Xiaoying Wu","doi":"10.1186/s13568-025-01906-5","DOIUrl":"10.1186/s13568-025-01906-5","url":null,"abstract":"<p><p>This study developed a novel antioxidant fusion protein Prx-LCA2 by conjugating peroxidase Prx with the LCA2 carrier derived from Escherichia coli heat-labile enterotoxin, aiming to achieve efficient intracellular delivery for oxidative damage remediation. The fusion protein Prx-LCA2 was successfully expressed in E. coli and purified. Fluorescence labeling demonstrated efficient cellular internalization of the fusion protein. In vitro, H₂O₂-induced oxidative stress in A431 cells was alleviated by Prx-LCA2 treatment, as evidenced by increased cell viability, reduced ROS levels, enhanced antioxidant enzyme activities, and decreased levels of MDA and PCG. In vivo, H₂O₂-induced skin oxidative damage in mice was significantly ameliorated by Prx-LCA2 treatment, including improvement in antioxidant enzyme activities and reduction in oxidative damage markers (MDA, PCG and 8-OHdG). Additionally, Prx-LCA2 increased HYP content in the skin, indicating improved collagen integrity. Histological analysis of mouse skin further confirmed the therapeutic efficacy of Prx-LCA2. The enterotoxin-derived carrier system exhibited excellent biosafety profile with no observed cytotoxicity or skin irritation. This microbial-based protein engineering strategy provides a promising platform for transdermal delivery of antioxidant therapeutics.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"94"},"PeriodicalIF":3.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12177121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and optimization of polyglutamic acid from Bacillus licheniformis: effect of low levels of gamma radiation.","authors":"Riham M Youssef, Reham Samir, Ola M Gomaa, Hala N ElHifnawi, Mohamed A Ramadan","doi":"10.1186/s13568-025-01897-3","DOIUrl":"10.1186/s13568-025-01897-3","url":null,"abstract":"<p><p>Polyglutamic acid (PGA), has several beneficial characteristics and advantageous uses. The microbial biopolymer is released from a variety of organisms, particularly Bacillus species. Soil samples from various sectors of Egypt were gathered and evaluated for the production of PGA. According to the current study results, isolate (L) was the most powerful producer and was identified by 16S rRNA as Bacillus licheniformis. A multi-factorial Plackett-Burman layout was used to optimize PGA bacterial synthesis. Mass spectrometry, thin-layer chromatography, Fourier transform infrared spectroscopy (FTIR), and amino acid analysis were employed to characterize the product. It was also investigated how the produced polymer was affected by low gamma irradiation doses. In comparison to Bacillus subtilis (ATCC6633), Bacillus licheniformis showed an over 33% inclination in PGA yield under optimal conditions. FTIR spectra demonstrated that PGA functional groups were successfully unchanged after exposure to low levels of gamma radiation similar to the doses given to cancer patients under radiotherapy. Thermal Gravimetric Analysis (TGA) showed 2 phase weight loss for gamma irradiated PGA, emphasizing that the polymer showed thermal stability after exposure to gamma radiation. These results were confirmed by Dynamic light scattering (DLS) and liquid chromatography mass spectrometry. Finally, the isolated Bacillus licheniformis strain can be considered as a prospective source of PGA for future industrial and medicinal uses. Additionally, this could be a promising result for the use of PGA as a drug carrier for cancer patients undergoing radiation therapy.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"93"},"PeriodicalIF":3.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bidirectional two-sample Mendelian randomization reveals causal link between genetic blood metabolites and tuberculosis.","authors":"Zhenyu Shi, Chenyi Zhao, Xiaowen Yu, Dingding Zhao, Yongqiang Li","doi":"10.1186/s13568-025-01901-w","DOIUrl":"10.1186/s13568-025-01901-w","url":null,"abstract":"<p><p>Tuberculosis (TB), caused by infectious agent Mycobacterium tuberculosis (Mtb) seriously poses a great threat to health. An array of metabolites generated by metabolic pathways are essential for Mtb pathophysiology. However, a specific causal relationship between TB and human metabolites remains indistinct. This study aimed to investigate the relationship between 1400 metabolites and TB by Mendelian randomization (MR) analysis. In this study, a total of 1400 metabolites were utilized as exposure factors, while TB-related data served as the outcomes. And TwoSampleMR package and R software were adopted to perform this MR analysis. Various regression fitting methods were employed to conduct MR analysis, including inverse variance weighted (IVW), MR-Egger, weighted median, simple mode, and weighted mode. In addition, potential biases arising from linkage disequilibrium and weak instrumental variables were considered. Metabolites that failed to meet the criteria in both the heterogeneity and pleiotropy tests were considered to have no substantial causal influence on the results, ensuring the robustness and reliability of our analysis. IVW analysis showed that six human metabolites exhibited a significant causal influence (P < 0.05) on TB. Among them, dodecanedioate, myristoleate (14:1n5), and 1-(1-enyl-palmitoyl)-2-arachidonoyl-GPE(p-16:0/20:4) demonstrated a strong causally positive effect on TB, indicating that with the increase of these metabolites, TB progressed robustly. Glycerol 3-phosphate, sphingomyelin (d18:1/20:2, d18:2/20:1, and d16:1/22:2), and 2-methylserine were significantly negatively associated with TB, an increase in these metabolites inhibited TB progression. This is the first time to reveal the causal effects of human metabolites on TB through MR, and the metabolites may be potential biomarkers candidate for TB diagnosis, and monitoring these metabolites might have great clinic significance for TB diagnosis and treatment in the future.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"92"},"PeriodicalIF":3.5,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the trimethylamine-degrading genes in the human gut microbiome.","authors":"Yi-Ran Chen, Li-Dan Chen, Lin-Jie Zheng","doi":"10.1186/s13568-025-01902-9","DOIUrl":"10.1186/s13568-025-01902-9","url":null,"abstract":"<p><p>Trimethylamine (TMA), produced by gut microbes, is a precursor to a risk factor for cardiovascular diseases. Currently, TMA-degrading bacteria in the human gut have rarely been studied. This study combined TMA-enriched cultures (from 104 young male stool samples) with metagenomic profiling to identify key microbial players of TMA degradation. The results showed that the contribution of Enterococcus to methane metabolism was significantly higher in TMA-enriched culture samples. The 68.58% up-regulation of dmd-tmd (dimethylamine/trimethylamine dehydrogenase) in the TMA-enriched group indicated that the anaerobic dehydrogenase pathway participated in TMA metabolism. Notably, we first identified that taxa containing dmd-tmd belonged to Christensenella timonensis. The up-regulation of genes involved in methanogenesis (M00563) as well as the significant enrichment of M00563 (Reporter Score = 2.223) indicated that the methanogenesis pathway may play a role. We constructed gene databases for genes involved in the anaerobic dehydrogenase pathway (1526 sequences for dmd-tmd, 1319 sequences for mauA, and 326 sequences for mauB, respectively) and the aerobic oxidation pathway (2146 sequences for tmm, 1445 sequences for tdm, and 1519 sequences for dmm, respectively) based on genomes from the Integrated Microbial Genome (IMG) database, most of which belong to Pseudomonadota. Screening gut metagenomes with these databases revealed low sequence identity (< 70%), possibly because of the underrepresentation of gut-specific genomes from IMG. This study links Christensenella timonensis to TMA degradation, providing potential targets for microbiota modulation and a gene-centric framework to advance the characterization of gut microbial TMA metabolism.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"91"},"PeriodicalIF":3.5,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory potential of Toxoplasma gondii lysate in C57BL/6 mouse model of autoimmune encephalomyelitis.","authors":"Maryam Hajizadeh, Seyedmousa Motavallihaghi, Elham Badakhsh, Tohid Kazemi, Abdol Sattar Pagheh, Kareem Hatam-Nahavandi, Ehsan Ahmadpour","doi":"10.1186/s13568-025-01900-x","DOIUrl":"10.1186/s13568-025-01900-x","url":null,"abstract":"<p><p>Multiple sclerosis (MS) is a debilitating autoimmune disease characterized by chronic inflammation and demyelination within the central nervous system. Immunotherapy is an essential part of managing MS symptoms and progression. This study aimed to evaluate the immunomodulatory effects of Toxoplasma gondii lysate antigens (TLA) in a C57BL/6 mouse model of experimental autoimmune encephalomyelitis (EAE). Eighteen mice were randomly assigned into three groups: healthy controls, EAE-induced mice, and EAE-induced mice treated with TLA. Clinical scores were recorded daily for all mice. To assess immune responses, mRNA and protein expression levels in isolated splenocytes were measured using quantitative real-time PCR and ELISA, respectively. In addition, histological analyses were performed to evaluate inflammation and demyelination across the experimental groups. TLA-treated mice exhibited significantly reduced clinical scores and demyelination compared to the untreated EAE group. The treatment also decreased inflammatory cell infiltration in CNS tissues. At the molecular level, TLA modulated cytokine expression by downregulating pro-inflammatory markers (IFN-γ, IL-17, RORγT) and upregulating anti-inflammatory and regulatory markers (IL-4, GATA3, FOXP3, TGF-β, TNF-α). TLA treatment demonstrated immunomodulatory and neuroprotective effects in EAE mice, suggesting its potential as a prophylactic or therapeutic agent in MS. These findings support further exploration of parasitic antigens in the context of autoimmune neuroinflammatory diseases.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"89"},"PeriodicalIF":3.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12158863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ochratoxin A reduction in wine fermentation: evaluating the potential of Lachancea thermotolerans.","authors":"Javier Vicente, Daniel Vidal, Wendu Tesfaye, Fernando Calderón, Fernando García, Santiago Benito","doi":"10.1186/s13568-025-01896-4","DOIUrl":"10.1186/s13568-025-01896-4","url":null,"abstract":"<p><p>Ochratoxin A is a mycotoxin commonly found in wine, primarily produced by fungal species from the Aspergillus and Penicillium genera. Due to its nephrotoxic, neurotoxic, immunotoxic, and carcinogenic properties, ochratoxin A contamination in wine is a significant concern for public health. This study investigates the potential of Lachancea thermotolerans in reducing ochratoxin A levels during wine fermentation, evaluating its fermentative performance and impact on key enological parameters. Fermentation trials with 32 L. thermotolerans strains demonstrated considerable variability in fermentation kinetics, ethanol production, and sugar consumption. The yeast exhibited strain-dependent variability in the production of organic acids, including succinic and lactic acid, leading to significant differences in total acidity and pH. Additionally, L. thermotolerans produced glycerol levels comparable to or exceeding those of Saccharomyces cerevisiae. The ability of L. thermotolerans to reduce ochratoxin A was highly strain-dependent, with reductions ranging widely. The most effective strains achieved ochratoxin A reductions exceeding those previously reported for S. cerevisiae. However, an inverse correlation was observed between ochratoxin A reduction and polyphenol retention, suggesting that strains with high ochratoxin A adsorption may also bind anthocyanins and polyphenols, affecting wine color and structure. These findings highlight L. thermotolerans as a promising non-Saccharomyces yeast for mitigating ochratoxin A contamination in wine while contributing positively to acidity modulation and sensory attributes. The study underscores the importance of strain selection to balance ochratoxin A detoxification with desirable enological properties, particularly in regions where contamination poses a significant challenge to wine safety and quality.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"90"},"PeriodicalIF":3.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12158886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Control of avocado anthracnose by carposphere-associated Kosakonia cowanii VG1 for agricultural applications.","authors":"Marco V Gallardo-Camarena, Frédérique Reverchon, Alfonso Méndez-Bravo, Mario A Torres-Acosta, Cuauhtémoc Licona-Cassani","doi":"10.1186/s13568-025-01894-6","DOIUrl":"10.1186/s13568-025-01894-6","url":null,"abstract":"<p><p>Avocado anthracnose, a devastating fungal disease caused by Colletotrichum gloeosporioides and Colletotrichum acutatum, represents the most significant postharvest challenge for avocado production. This study evaluates the biocontrol potential of bacterial strains isolated from different niches within the avocado ecosystem, including fruits, roots, trunks and nectar. Among these isolates, the bacterium Kosakonia cowanii VG1, isolated from avocado carposphere, demonstrated the highest in vitro antagonistic activity, inhibiting mycelial growth and spore germination of both Colletrotrichum species by 70% and 65%, respectively. In vivo experiments with K. cowanii VG1 reduced disease severity by 60% in avocado fruits compared to untreated controls. Genomic analysis of K. cowanii VG1 revealed the absence of virulence factors, ensuring safety for human health. Additionally, fermentation analysis demonstrated that K. cowanii VG1 can be efficiently cultured at large scales. Additionally, a techno-economic evaluation showed that its production costs are competitive, ranging from $0.08-$0.15 per dose. These findings highlight the potential of K. cowanii VG1 as a promising, safe, and cost-effective biocontrol agent for managing avocado anthracnose, offering a sustainable alternative to synthetic fungicides.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"88"},"PeriodicalIF":3.5,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of probiotic treatment on the intestinal microbial community of Haliotis diversicolor.","authors":"Ruixuan Wang, Juan Wang, Daguang Tang, Bing Li, Jianjian Huang, Xiaozhi Lin, Yun Li, Wenju Xu, Weifeng Gao, Jiangyong Wang, Hui Zhu","doi":"10.1186/s13568-025-01885-7","DOIUrl":"10.1186/s13568-025-01885-7","url":null,"abstract":"<p><p>Probiotic treatment is an effective method for enhancing growth performance and improving intestinal flora in aquaculture species. This study examined the effects of three candidate-probiotics (Bacillus, photosynthetic bacteria, and Lactobacillus) on the rate of weight gain and the intestinal flora of abalone juveniles. Haliotis diversicolor was fed a probiotic-supplemented diet for 30 days. The abalones fed with Lactobacillus showed a more significant weight gain rate than those in the Bacillus, photosynthetic bacteria, and control groups. Through 16S rRNA high-throughput sequencing, 12,490 amplicon sequence variants (ASVs) were obtained from the abalone intestinal tract microbiome. After a short feeding period (5 days), the Bacillus and photosynthetic bacteria-treated groups showed an increased abundance of Proteobacteria in the abalone digestive tract. In the Lactobacillus-treated group, the quantity of Proteobacteria decreased, and the abundance of Bacteroidota increased. After 30 days of feeding, the abundance of Proteobacteria and Bacteroidetes at the phylum level was more significant in the Bacillus-treated group and photosynthetic bacteria-treated group than in the controls. The Lactobacillus-treated group showed an increase in the quantity of Proteobacteria and Chloroflexi. The dominant flora of the three probiotic treated groups changed slightly with respect to the control group. After a short period of feeding (5 days), the abundance of Rhodobacteraceae (at the genus level) in the abalone digestive tract increased in the Bacillus- and photosynthetic bacteria-treated groups, whereas in the Lactobacillus-treated group, Rhodobacteraceae decreased, and Maribacter increased in abundance. After 30 days of feeding, Bacteroidetes and Ruegeria were higher in the Bacillus-treated group than in the control group. Marinirhabdus and Bacteroidetes increased in the photosynthetic bacteria-treated group, and Roseivivax and Ruegeria increased in the Lactobacillus-treated group. The three probiotic-treated groups had higher microbial diversity than the control group. Therefore, our findings confirmed that adding Bacillus, photosynthetic bacteria, and Lactobacillus to the abalone diet increased abalones' weight gain rate and altered their intestinal microbiome composition.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"87"},"PeriodicalIF":3.5,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organic fertilizers and Azotobacter: effects on onion growth, yield, metabolites, and soil fertility.","authors":"Waleed M Ali, Sadoun M E Sultan, Ahmed M Ali, Hassan M Al-Sayed, Mohamed Ahmed Mahmoud, Hanan G Ismail, Islam I Teiba, Ahmed Fathy Yousef","doi":"10.1186/s13568-025-01895-5","DOIUrl":"10.1186/s13568-025-01895-5","url":null,"abstract":"","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"86"},"PeriodicalIF":3.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology of lumpy skin disease in Northeast India and a new method for rapid field diagnosis.","authors":"Samir Das, Kandhan Srinivas, Arockiasamy Arun Prince Milton, Sabia Khan, Lavinia Wahlang, Hosterson Kylla, Gundallahalli Bayyappa Manjunatha Reddy, Sharanagouda S Patil, Erica Lawai Lyngdoh, Pebam Chandrima Devi, Sandeep Ghatak, Kekungu-U Puro, Arnab Sen","doi":"10.1186/s13568-025-01891-9","DOIUrl":"10.1186/s13568-025-01891-9","url":null,"abstract":"<p><p>Lumpy Skin Disease (LSD), a viral disease affecting cattle and related species, entered India in 2019, with first outbreak reported in Odisha, and has since caused significant economic losses to the Indian livestock sector. Following its entry, the disease spread rapidly to various states, including the northeastern region which is known for its porous borders. A total of 56 clinical samples (whole blood, skin scrapings, nasal swab, skin lesions and serum) were collected from various outbreaks of LSD in Meghalaya. World Organisation for Animal Health (WOAH)-recommended probe-based real-time polymerase chain reaction (PCR) targeting LSDV126 region detected Lumpy skin disease virus (LSDV) in 42 out of 56 clinical samples. Additionally, 182 contemporary serum samples were screened using commercial ELISA kit which indicated an apparent seroprevalence of 11.54% (95% confidence interval [CI] 7.7-17%) and a true prevalence of 12.3% (95% CI 7.86-18.53%). Three representative samples were subjected to partial sequencing-based phylogenetic analysis targeting the LSDVp32 and LSDV117 regions which confirmed the diagnosis of LSD and also revealed circulation of the Kenyan field strain-associated LSDV. Furthermore, a novel isothermal approach exploiting Saltatory Rolling Circle Amplification (SRCA) mechanism was also explored with WOAH-recommended conventional PCR primers which yielded results comparable to real-time PCR-based diagnostic methods with a minimum detection limit of 10 copies/µL of the standard plasmid. The availability of this rapid and reliable diagnostic assay could be harnessed for early diagnosis of LSD, especially in resource-limited and field laboratory settings and ultimately aid in the timely implementation of control strategies.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"85"},"PeriodicalIF":3.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}