AMB Express最新文献

{"title":"Clinical correlation study of non-tuberculous mycobacterial isolates from bronchoalveolar lavage fluid.","authors":"Hongye Ning, Guiqing He, Yanhong Mei, Jiandan Yu, Jichan Shi, Xiaoya Cui, Chaochao Qiu, Xiangao Jiang","doi":"10.1186/s13568-025-01842-4","DOIUrl":"10.1186/s13568-025-01842-4","url":null,"abstract":"<p><p>Non-tuberculous mycobacterial (NTM) infections have emerged as a significant public health concern, posing a threat to human health. This study aims to identify various NTM strains from bronchoalveolar lavage fluid, assess their drug resistance profiles, and investigate the risk factors associated with NTM disease. Gene chip technology was used to identify NTM strains. The broth microdilution method assessed the drug sensitivity of isolated NTM pathogenic bacteria, determining their minimum inhibitory concentrations (MICs). Logistic regression analysis identified potential risk factors for NTM disease. Results showed the slow-growing NTM strains isolated from bronchoalveolar lavage fluid to be predominantly Mycobacterium avium and Mycobacterium intracellulare, accounting for 32.05% and 29.49% of the isolates, respectively. The rapidly growing NTM strains were mainly Mycobacterium chelonae and Mycobacterium abscessus, each constituting 25.64% of the isolates. Mycobacterium avium was found to be sensitive to clarithromycin, while linezolid demonstrated high antibacterial efficacy against Mycobacterium intracellulare. In drug susceptibility testing of Mycobacterium chelonae and Mycobacterium abscessus, amikacin exhibited the highest sensitivity, followed by clarithromycin. For patients with NTM-positive cultures, the risk factors for NTM lung disease included age (45-60 years, > 60 years), a smoking history exceeding 10 years, chronic obstructive pulmonary disease (COPD), bronchiectasis, immunocompromised status, and the presence of thin-walled pulmonary cavities. Collectively, this study elucidates the distribution of NTM strains, their drug susceptibility profiles, and key risk factors for NTM lung disease, highlighting the need for proactive screening, early intervention, and targeted preventive strategies to improve diagnosis and optimize treatment outcomes.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"40"},"PeriodicalIF":3.5,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virulence genes, efflux pumps, and molecular typing of Klebsiella pneumoniae isolates from North Iran.","authors":"Arta Hafezi Birgani, Hamid Reza Goli, Seyed Davar Siadat, Abolfazl Fateh, Vajihe Sadat Nikbin, Fatemeh Sakhaee, Fatemeh Ashrafian, Mohammad Reza Haghshenas, Mehrdad Gholami","doi":"10.1186/s13568-025-01845-1","DOIUrl":"10.1186/s13568-025-01845-1","url":null,"abstract":"<p><p>Resistant and virulent strains of Klebsiella pneumoniae (K. pneumoniae) are rapidly disseminated among both hospitalized patients and communities, therefore, the identification of the genes responsible for virulence and resistance, along with the clonal relatedness of these strains, could be beneficial in the management of the dissemination of these strains among patients. The aim of the present study was to assess antibiotic susceptibility, virulence and resistance genes, as well as the genetic relatedness of K. pneumoniae strains isolated from patients admitted to four hospitals in Mazandaran, Iran. A total of 95 K. pneumoniae were gathered from hospitalized patients. All isolates were confirmed using biochemical and conventional microbiological methods, followed by the assessment of susceptibility patterns through disk diffusion and the detection of resistance and virulence genes using conventional PCR. The genetic diversity of clinical isolates was determined using the Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) technique. The resistance frequencies varied, with the highest being for ampicillin/sulbactam (95.8%) and the lowest for fosfomycin (3.2%). Only one strain displayed a non-MDR profile against all antibiotics tested. Virulence-associated genes were detected, such as mrkD (90.5%), fimH (80%), entB (92.6%), iutA (25.3%), and ybtS (68.4%). Genes associated with efflux pumps and outer membrane porins included acrAB (98.9%), tolC (95.8%), mdtK (83.2%), ompK35 (95.8%), and ompK36 (92.6%). Based on ERIC-PCR patterns with a 90% similarity, the isolates were categorized into 17 distinct clusters. While the majority of isolates had a same profile and were grouped in the predominant pattern, 11 isolates were identified as singletons. Our study indicates that the prevalence of MDR K. pneumoniae carrying virulence genes and exhibiting close relatedness underscores the urgent need for effective strategies to control and prevent infections caused by K. pneumoniae.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"36"},"PeriodicalIF":3.5,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Best among the key molecular diagnostic markers of bacterial vaginosis.","authors":"Tongyang Deng, Xiangquan Song, Qiumei Liao, Ying Zheng, Hong Sun, Lianzhen Zhang, Xuejia Chen","doi":"10.1186/s13568-025-01847-z","DOIUrl":"10.1186/s13568-025-01847-z","url":null,"abstract":"<p><p>To assess bacterial vaginosis (BV)-related primary molecular diagnostic markers of Lactobacillus crispatus, Gardnerella vaginalis, Fannyhessea vaginae, bacterial vaginosis-associated bacteria 2 (BVAB-2), Megasphaera-1 and Megasphaera-2 and to discover molecular diagnostic indicators of BV with the most economic value for the efficient diagnosis of BV.All vaginal secretion specimens, including 122 BV-positive cases and 130 BV-negative controls were collected. First, quantitative polymerase chain reaction (PCR) was used to determine the levels of above the six bacteria. Then, the detection rates, sensitivity, specificity, diagnostic threshold, and receiver operating characteristic (ROC) curve were compared. Megasphaera-1 and Megasphaera-2 were detected in the BV-positive group, with a low detection rate of 35.25% and 19.67% respectively. The sensitivity and specificity of the above four bacteria were 95.90%/72.31%, 82.79%/92.48%, 72.13%/95.38%, and 56.56%/94.62% respectively, using the cut-off value for the diagnosis of BV. When combinations of L. crispatus with G. vaginalis, F. vaginae, and BVAB-2 were performed respectively, their sensitivity and specificity were 99.29%/97.79%, 98.86%/98.72%, and 98.22%/98.51% in sequence.It is difficult to diagnose BV using only one species, however, combinations of L. crispatus with G. vaginalis or F. vaginae showed a better diagnostic effect, particularly with the combination of L. crispatus and G. vaginalis.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"35"},"PeriodicalIF":3.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A method for creating in-frame insertions of fluorescent proteins in non-model gram-negative bacteria.","authors":"Darshan Chandramowli, Bart Devreese","doi":"10.1186/s13568-025-01844-2","DOIUrl":"10.1186/s13568-025-01844-2","url":null,"abstract":"<p><p>The use of fluorescent proteins to study protein expression and localisation has become common practice in the life sciences. While methods to create gene fusions and replacements with fluorescent proteins in model organisms have rapidly developed, there exist far fewer well-established protocols applicable to non-model bacteria. Here, we present a comprehensive account of an allelic-exchange-based mutagenesis strategy using the I-SceI endonuclease in a clinical strain of S. maltophilia. We demonstrate the use of this strategy for the creation of in-frame insertions of fluorescent proteins and entire gene replacements for the purposes of studying protein localisation and expression. This protocol requires minimal setup, and once optimised, can produce mutants in a matter of weeks. We expect this strategy to be of use for laboratories working with poorly-characterised strains and/or bacteria for which information is scarce.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"34"},"PeriodicalIF":3.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reprofiling lamivudine as an antibiofilm and anti-pathogenic agent against Pseudomonas aeruginosa.","authors":"Othman Yahya Alyahyawy, Raafat M Munshi, Shaimaa M Badr-Eldin, Hibah Mubarak Aldawsari, Walaa Abualsunun, Hisham A Abbas, Ibrahim M Salem, Wael A H Hegazy, Shaimaa I Nazeih","doi":"10.1186/s13568-025-01835-3","DOIUrl":"10.1186/s13568-025-01835-3","url":null,"abstract":"<p><p>Resistance to antibiotics is a critical growing public health problem that needs urgent action to combat. To avoid the stress on bacterial growth that evokes the development of resistance, anti-virulence agents can be an attractive strategy as they do not target bacterial growth. There are FDA approved drugs have been screened for their anti-virulence activities. Lamivudine (LAM) is a synthetic nucleoside analogue used as an antiretroviral in treatment of HIV and can be used in treatment of HBV. The present study aimed to assess the anti-virulence activities of LAM against a clinically important pathogen Pseudomonas aeruginosa. The LAM's antibiofilm and anti-virulence activities were evaluated. The impact of LAM on the quorum sensing (QS) systems which control the production of these virulence factors was assessed virtually and by quantification of the expression of QS-encoding genes. Furthermore, in vivo mice protection assay was conducted to attest the LAM's anti-pathogenic activity. The current findings elaborated the promising anti-pathogenic and anti-QS activities of LAM. LAM interfered with biofilm formation in P. aeruginosa PAO1 strain. Moreover, swarming motility and production of pyocyanin and protease were significantly diminished. At the molecular level, LAM downregulated the QS-encoding genes LasI, LasR, RhlR, PqsA and PqsR. Additionally, the detailed in silico docking and molecular simulation studies showed the considered high LAM's ability to bind and hinder the QS receptors in the P. aeruginosa. In an agreement with in vitro and in silico, the in vivo results showed the LAM full protection of mice against P. aeruginosa. In conclusion, LAM could be repurposed to be employed as adjunct therapy with traditional antibiotics for treating serious pseudomonal infections.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"33"},"PeriodicalIF":3.5,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Staphylococcal SplA and SplB serine proteases target ubiquitin(-like) specific proteases.","authors":"Felix L Glinka, Ole Schmöker, Abhishek K Singh, Leif Steil, Christian Hentschker, Uwe Völker, Dominique Böttcher, Michael Lammers, Clemens Cammann, Ulrike Seifert, Elke Krüger, Michael Naumann, Barbara M Bröker, Uwe T Bornscheuer","doi":"10.1186/s13568-025-01841-5","DOIUrl":"10.1186/s13568-025-01841-5","url":null,"abstract":"<p><p>Staphylococcus aureus is a Gram-positive opportunistic pathogen that has colonized nearly 30% of the human population and can cause life-threatening infections. S. aureus exports a variety of virulence factors, such as a novel set of extracellular serine protease-like proteins (Spls). Spls are expressed by most clinical isolates of S. aureus, but their pathophysiological substrates and role during the infection are largely unknown. Here we characterized the substrate and cleavage specificity of recombinantly expressed SplA and SplB proteins. We identified a group of ubiquitin or ubiquitin-like modifying enzymes including deubiquitinating enzymes from human as well as from bacterial sources to be so far unknown SplA and SplB substrates. Distinct cleavage sites within these substrates for SplA (YLY^↓T, FMY^↓N) and SplB (VCD^↓S) were identified by mass spectrometry and confirmed by site-directed mutagenesis of the target proteins. Since many cellular immune signaling pathways are tightly regulated by ubiquitination, the specific cleavage of ubiquitin modifying enzymes strongly suggests a specific role of Spls in manipulating immune signaling and in competing with other bacteria.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"32"},"PeriodicalIF":3.5,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of ferroptosis-related key genes in tuberculosis by bioinformatics methods.","authors":"Wenchuan Zhang, Dongxue Zhu, Hong Jiang, Limei Wang","doi":"10.1186/s13568-025-01839-z","DOIUrl":"10.1186/s13568-025-01839-z","url":null,"abstract":"<p><p>Tuberculosis, induced by Mycobacterium tuberculosis (Mtb), continues to pose a significant global public health challenge. Ferroptosis has emerged as a pivotal factor in tuberculosis pathogenesis, however, the mechanism has not yet been fully clarified. Therefore, the aim of this study was to hypothesize and validate potential ferroptosis-related genes in Mtb infection through bioinformatics analysis, thereby offering insights for further investigation. The mRNA microarray expression profile datasets were sourced from the Gene Expression Omnibus. The differentially expressed genes (DEGs) were derived using GEO2R. Subsequently, the shared DEGs between the GSE174566 and GSE227851 datasets were intersected with the genes in the ferroptosis database. The ferroptosis-associated shared DEGs (Ferr-sDEGs) were validated in the GSE20050 dataset. They were subjected to PPI, Cytoscape and Friends analysis, the infiltration correlation of immune cells and qRT-PCR. A total of 11 Ferr-sDEGs were identified, and 9 genes were validated. These analyses revealed that the key Ferr-sDEGs contributed to ferroptosis during Mtb infection and these key Ferr-sDEGs were relatively independent, implying that ferroptosis may be triggered by various mechanisms. Concurrently, the infiltration and correlation analysis demonstrated that multiple types of immune cells could be activated by the key Ferr-sDEGs. Ultimately, qRT-PCR validated that the expression levels of key Ferr-sDEGs. In conclusion, ferroptosis serves a pivotal function in the pathogenesis of tuberculosis. IL1B, PTGS2, TNFAIP3, HMOX1, SOCS1, CD82, and NUPR1 may be vital genes associated with the ferroptosis induced by Mtb infection.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"31"},"PeriodicalIF":3.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11845653/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 12-year surveillance study on distribution and antimicrobial resistance of gram-positive bacteria in Iran.","authors":"Shayan Salehi Rad, Hassan Pourmoshtagh, Sahar Sabour, Shiva Nazari, Sina Sohrabizadeh, Taher Azimi","doi":"10.1186/s13568-025-01833-5","DOIUrl":"10.1186/s13568-025-01833-5","url":null,"abstract":"<p><p>Strengthening knowledge through surveillance and research is one of the main steps in treating antibiotic-resistant infections. The present study was therefore designed to analyse long-term antimicrobial resistance trends over the 12 years in Iran. Data from the patient's clinical specimens were collected over 12 years from January 2012 until December 2023. Antimicrobial susceptibility patterns of gram-positive bacteria (GPB) were identified using the Kirby-Bauer disk diffusion method (DDM) and resistance proportions to most commonly used antibiotics were calculated for each GPB. All data were analysed using descriptive statistics. A total of 30,548 cultures were positive for GPB. Staphylococcus aureus (S. aureus) with 59.5% and coagulase-negative staphylococci (CoNS) with 26.6% frequently isolated from blood specimens. S. aureus was the frequently isolated organism in ≥ 6 years of age groups. S. aureus isolates showed the highest and lowest resistance rates to penicillin (91.8%) and vancomycin (1.1%), respectively. Nitrofurantoin (10.5%) was the most effective antimicrobial agent on Enterococcus spp. Time trends in antibiotic resistance revealed a sharp increase for several antibiotics: from 33.9% in 2013 to 54.5% in 2023 for gentamicin; from 51.2% in 2013 to 76.4% in 2023 for ceftriaxone; from 34.5% in 2013 to 54.8% in 2023 for imipenem; and from 66.7% in 2012 to 81.8% in 2023 for cefixime. The present research suggests that vancomycin and nitrofurantoin are effective antibiotics against infections caused by Staphylococcus and Enterococcus spp., respectively. This data revealed that the continuous monitoring of antimicrobial resistance (AMR) patterns is necessary for selecting the suitable drug.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"30"},"PeriodicalIF":3.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11825410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the Withania somnifera alkaloids potential for cure of neurodegenerative disease: an in-silico study.","authors":"Ruili Chen, Dongdong Zhang, Anis Ahmad Chaudhary, Salah-Ud-Din Khan, Saeed Vohra, Yusra Vohra, Nini Li, Shahanavaj Khan","doi":"10.1186/s13568-025-01826-4","DOIUrl":"10.1186/s13568-025-01826-4","url":null,"abstract":"<p><p>This study investigates the interaction of alkaloids of Withania somnifera with Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), an enzyme implicated in the pathology of various neurodegenerative diseases. Initially, the Withania somnifera phytochemicals were optimised then molecular dockings were performed. The molecular docking results identified key interactions between these alkaloids and active sites of the enzyme. Several alkaloids from Withania somnifera exhibited better binding affinities to GAPDH such as Withanolides G, I, and K with binding affinity of -10.1, -10, and - 10 kcal/mol. The binding orientations and stability of these withanolide derivatives complexes GAPDH were investigated through comprehensive molecular dynamics simulations over 100 nanoseconds. The molecular dynamics simulations revealed stable interactions over the simulation period, suggesting a strong binding propensity. Furthermore, we determined the free energy binding profiles with GAPDH using BAR method, whichresulted in improved free energybinding forWithanolide-G and I.The results envisage that withanolides showed excellent bindings and stable interaction with the GAPDH, making them an effective therapeutic agent. The current study providesan excellent platform for developing new therapeutic agents derived from Withania somniferaagainst neurodegenerative diseasesassociated with GAPDH malfunction. These novel results open novel avenues for future research in drug discovery, particularly in developing novel treatments for Alzheimer's, Parkinson's, and other neurodegenerative diseases where GAPDH dysfunction plays crucial roles.Furthermore, experimental validation is required to validate the results of the current study.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"29"},"PeriodicalIF":3.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11825968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Substrate-dependent lipid and β-carotene production in engineered Yarrowia lipolytica: a comparative study.","authors":"Raúl Robles-Iglesias, Jean-Marc Nicaud, María C Veiga, Christian Kennes","doi":"10.1186/s13568-025-01834-4","DOIUrl":"10.1186/s13568-025-01834-4","url":null,"abstract":"<p><p>This study evaluates the influence of various substrates (glucose, glycerol, and acetic acid) on the growth and metabolite production of Yarrowia lipolytica in fed-batch bioreactors. The primary aim is to understand how substrate choice impacts lipid and β-carotene production, critical for bioenergy and bioproducts. The study demonstrates that the choice of substrate significantly influences biomass yield, lipid content, and β-carotene levels. Among the substrates tested, glycerol yielded the highest biomass concentration of 5.31 g/L. Glucose led to the highest lipid content, with a yield of 35.8% (g lipids/g biomass), while acetic acid resulted in the highest lipid concentration, reaching 1.42 g/L. In terms of β-carotene production, glucose showed the highest content per cell at 63.3 mg/g, whereas glycerol led to the highest overall concentration of 202 mg/L. These findings highlight Y. lipolytica's versatility and potential as a flexible platform to produce lipids and β-carotene, which are essential for developing sustainable biofuels and bioproducts. The study underscores the significant variations in metabolite production based on substrate choice, emphasizing on the importance of tailored strategies to optimize industrial applications. Further research may explore optimizing fermentation conditions to enhance production yields, making this yeast a viable option for various biotechnological applications.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"27"},"PeriodicalIF":3.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11807037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}