AMB Express最新文献

{"title":"Effects of probiotic treatment on the intestinal microbial community of Haliotis diversicolor.","authors":"Ruixuan Wang, Juan Wang, Daguang Tang, Bing Li, Jianjian Huang, Xiaozhi Lin, Yun Li, Wenju Xu, Weifeng Gao, Jiangyong Wang, Hui Zhu","doi":"10.1186/s13568-025-01885-7","DOIUrl":"10.1186/s13568-025-01885-7","url":null,"abstract":"<p><p>Probiotic treatment is an effective method for enhancing growth performance and improving intestinal flora in aquaculture species. This study examined the effects of three candidate-probiotics (Bacillus, photosynthetic bacteria, and Lactobacillus) on the rate of weight gain and the intestinal flora of abalone juveniles. Haliotis diversicolor was fed a probiotic-supplemented diet for 30 days. The abalones fed with Lactobacillus showed a more significant weight gain rate than those in the Bacillus, photosynthetic bacteria, and control groups. Through 16S rRNA high-throughput sequencing, 12,490 amplicon sequence variants (ASVs) were obtained from the abalone intestinal tract microbiome. After a short feeding period (5 days), the Bacillus and photosynthetic bacteria-treated groups showed an increased abundance of Proteobacteria in the abalone digestive tract. In the Lactobacillus-treated group, the quantity of Proteobacteria decreased, and the abundance of Bacteroidota increased. After 30 days of feeding, the abundance of Proteobacteria and Bacteroidetes at the phylum level was more significant in the Bacillus-treated group and photosynthetic bacteria-treated group than in the controls. The Lactobacillus-treated group showed an increase in the quantity of Proteobacteria and Chloroflexi. The dominant flora of the three probiotic treated groups changed slightly with respect to the control group. After a short period of feeding (5 days), the abundance of Rhodobacteraceae (at the genus level) in the abalone digestive tract increased in the Bacillus- and photosynthetic bacteria-treated groups, whereas in the Lactobacillus-treated group, Rhodobacteraceae decreased, and Maribacter increased in abundance. After 30 days of feeding, Bacteroidetes and Ruegeria were higher in the Bacillus-treated group than in the control group. Marinirhabdus and Bacteroidetes increased in the photosynthetic bacteria-treated group, and Roseivivax and Ruegeria increased in the Lactobacillus-treated group. The three probiotic-treated groups had higher microbial diversity than the control group. Therefore, our findings confirmed that adding Bacillus, photosynthetic bacteria, and Lactobacillus to the abalone diet increased abalones' weight gain rate and altered their intestinal microbiome composition.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"87"},"PeriodicalIF":3.5,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12126437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organic fertilizers and Azotobacter: effects on onion growth, yield, metabolites, and soil fertility.","authors":"Waleed M Ali, Sadoun M E Sultan, Ahmed M Ali, Hassan M Al-Sayed, Mohamed Ahmed Mahmoud, Hanan G Ismail, Islam I Teiba, Ahmed Fathy Yousef","doi":"10.1186/s13568-025-01895-5","DOIUrl":"10.1186/s13568-025-01895-5","url":null,"abstract":"","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"86"},"PeriodicalIF":3.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology of lumpy skin disease in Northeast India and a new method for rapid field diagnosis.","authors":"Samir Das, Kandhan Srinivas, Arockiasamy Arun Prince Milton, Sabia Khan, Lavinia Wahlang, Hosterson Kylla, Gundallahalli Bayyappa Manjunatha Reddy, Sharanagouda S Patil, Erica Lawai Lyngdoh, Pebam Chandrima Devi, Sandeep Ghatak, Kekungu-U Puro, Arnab Sen","doi":"10.1186/s13568-025-01891-9","DOIUrl":"10.1186/s13568-025-01891-9","url":null,"abstract":"<p><p>Lumpy Skin Disease (LSD), a viral disease affecting cattle and related species, entered India in 2019, with first outbreak reported in Odisha, and has since caused significant economic losses to the Indian livestock sector. Following its entry, the disease spread rapidly to various states, including the northeastern region which is known for its porous borders. A total of 56 clinical samples (whole blood, skin scrapings, nasal swab, skin lesions and serum) were collected from various outbreaks of LSD in Meghalaya. World Organisation for Animal Health (WOAH)-recommended probe-based real-time polymerase chain reaction (PCR) targeting LSDV126 region detected Lumpy skin disease virus (LSDV) in 42 out of 56 clinical samples. Additionally, 182 contemporary serum samples were screened using commercial ELISA kit which indicated an apparent seroprevalence of 11.54% (95% confidence interval [CI] 7.7-17%) and a true prevalence of 12.3% (95% CI 7.86-18.53%). Three representative samples were subjected to partial sequencing-based phylogenetic analysis targeting the LSDVp32 and LSDV117 regions which confirmed the diagnosis of LSD and also revealed circulation of the Kenyan field strain-associated LSDV. Furthermore, a novel isothermal approach exploiting Saltatory Rolling Circle Amplification (SRCA) mechanism was also explored with WOAH-recommended conventional PCR primers which yielded results comparable to real-time PCR-based diagnostic methods with a minimum detection limit of 10 copies/µL of the standard plasmid. The availability of this rapid and reliable diagnostic assay could be harnessed for early diagnosis of LSD, especially in resource-limited and field laboratory settings and ultimately aid in the timely implementation of control strategies.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"85"},"PeriodicalIF":3.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of predictors for bacterial meningitis diagnosis based on transcriptomics and genetic analysis.","authors":"Hexiang Jiang, Xibing Yu, Jingyan Fan, Houhui Song, Yang Yang","doi":"10.1186/s13568-025-01893-7","DOIUrl":"10.1186/s13568-025-01893-7","url":null,"abstract":"<p><p>Bacterial meningitis (BM) requires rapid intervention, especially in immunocompromised populations. Understanding early immune responses is crucial, as they precede clinical symptoms; however, comprehensive studies remain limited. This research investigates immune-related genes to improve BM diagnosis and treatment. Mendelian randomization, differential gene expression analysis, and co-expression network analysis identified key genes associated with BM. Immune cell ratio calculations and infiltration analyses demonstrated altered immune cell proportions. Spearman correlation analysis revealed relationships between gene expression and immune cell types. Single-cell RNA sequencing, gene set enrichment analysis, and pseudotime analysis explored changes in gene expression and cell proportions across disease stages, focusing on the roles of key genes in specific immune cells. Ring Finger Protein 144B (RNF144B) was identified as a risk gene predominantly expressed in monocytes and neutrophils. Conversely, FYN Proto-Oncogene (FYN) was identified as a protective gene primarily associated with NKT cells. During BM onset, increased RNF144B expression positively correlated with elevated neutrophil levels, while reduced FYN expression correlated with decreased NKT cell levels. During remission and recovery, RNF144B expression and neutrophil proportions decreased, whereas FYN expression and NKT cell proportions increased. NKT cells appeared to play a protective role, with FYN potentially modulating T-cell receptor function in these cells, thereby reducing BM risk. RNF144B and FYN expression exhibit opposing trends in peripheral blood across BM stages, suggesting their potential as biomarkers for diagnosis and monitoring. These findings provide a valuable reference for early intervention strategies and personalized treatment approaches tailored to specific disease stages in the clinic.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"84"},"PeriodicalIF":3.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of UV-C radiation on chemical profile and pharmaceutical application in vitro of Aloe vera oil.","authors":"Sulaiman A Alsalamah, Mohammed Ibrahim Alghonaim, Tarek M Abdelghany, Mutasem S Almehayawi, Samy Selim, Mohanned T Alharbi","doi":"10.1186/s13568-025-01884-8","DOIUrl":"10.1186/s13568-025-01884-8","url":null,"abstract":"<p><p>The uncontrolled exposure to ultra violet (UV) radiation have many adverse impacts on human health. UV could be divided into three main classes according to wave length range where UV-C (200-280) is one of these classes. Aloe vera oil is a common product in markets with many cosmetic and medicinal applications. The purpose of this investigation is to expose the oil to UV-C at a dose of 17.0 mJ/cm² (254 nm) for 0, 30, and 60 min to examine the impact of change of exposure periods on the alteration in chemical composition of Aloe vera oil as well as its various biological activities. GC-MS examination of various oil forms reveled the proportional elevation in the number of compounds in the oil with increasing time of exposure to UV-C where linoleic acid ethyl ester and ethyl oleate were the most common compounds in oil forms with emergence of trace molecules and their derivatives from various chemical classes especially in the exposed oil to radiation especially after 60 min. The antimicrobial action towards Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumonia, and Salmonella typhi) and fungi (Aspergillus niger and Candida albicans) reflecting the successive to boost the activity versus examined microbes except for A. niger with inhibition zones ranged from 16 ± 1 to 24 ± 1mm, 18 ± 1 to 25 ± 1mm, and 22 ± 1 to 26 ± 1 mm using unexposed A. vera oil, exposed A. vera oil to UV for 30, and 60 min, respectively against tested microorganisms. Moreover, exposure to radiation at various time points resulted in substantial reduction of minimal inhibitory concentration (MIC) as well as minimal bactericidal concentration (MBC) for the applied oil forms. For instance the MIC values were 62.5 ± 0.1, 31.25 ± 0.1, and 15.62 ± 0.1 µg/ml for S. aureus, 125 ± 0.2, 62.5 ± 0.2, and 31.25 ± 0.2 µg/ml for B. subtilis, moreover MBC values were 125 ± 0.1, 125 ± 0.2, and 31.25 ± 0.3 µg/ml for S. aureus, 250 ± 0.3, 125 ± 0.2, and 31.25 ± 0.3 µg/ml for B. subtilis using unexposed A. vera oil, exposed A. vera oil to UV for 30, and 60 min, respectively. There is a notable improvement of anti-biofilm action of examined oil especially upon using 25% of MBC. Furthermore, antioxidant, anti-inflammatory, anticancer, and wound healing capabilities of the tested oils dramatically raised with increase time of exposure. Future studies are required to detect the maximal beneficial gain upon exposure to various natural oils UV-C.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"83"},"PeriodicalIF":3.5,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Silver/Zinc@Lignin nanoparticles using peanut shells: photocatalytic and biological activity.","authors":"Mahshid Shafiee, Zahra Sabouri, Asma Mostafapour, Majid Darroudi","doi":"10.1186/s13568-025-01892-8","DOIUrl":"10.1186/s13568-025-01892-8","url":null,"abstract":"<p><p>In this paper, zinc and silver-doped lignin nanoparticles (Ag/Zn@Lignin nanoparticles) were synthesized in two steps using peanut outer shells. The optical properties of NPs were analyzed through UV-Vis and PL measurements, while X-ray analysis approved their crystal structure. The surface characteristics and chemical composition of NPs were identified using FTIR analysis. TEM images of Ag/Zn@Lignin nanoparticles showed spherical morphology with an average size of about 22.71 nm. The antibacterial activity of Ag/Zn@Lignin nanoparticles was assessed against gram-negative and gram-positive bacteria, and the results exhibited strong antibacterial properties against the pathogenic strain compared to the standard. The MTT test results indicated that Ag/Zn@Lignin nanoparticles were more sensitive against cancer cells (Huh-7) in comparison with normal cells (L929), and IC₅₀ values were reported as 122.9 and 389 µg/mL in order. Also, photocatalytic effects displayed the high ability of Ag/Zn@Lignin nanoparticles to degrade organic pigments such as Rhodamine B (98.8%, RhB) and Methylene blue (98.8%, MB) under UV light.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"82"},"PeriodicalIF":3.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12106268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the benefits of seed oils: a comprehensive study on their role in functional foods.","authors":"Marwa A Kamel, Amira A Gamal, Sayeda A Abdelhamid, Marwa M El-Said, Tamer M El-Messery, Hamdy A Zahran","doi":"10.1186/s13568-025-01875-9","DOIUrl":"10.1186/s13568-025-01875-9","url":null,"abstract":"<p><p>There has been a growing interest in functional foods in recent years to improve health and boost immunity, particularly since the COVID-19 pandemic, which reflects their significant role in promoting health and preventing various diseases, especially metabolic disorders. This study investigated the antimicrobial, antioxidant, anticoagulant, and prebiotic activities of six different oils: Calotropis procera oil (CPO), Chia seed oil (CSO), Moringa oil (MO), Neem oil (NO), Black seed oil (BSO), and Wheat germ oil (WGO) and their potential applications in health and nutrition. The DPPH and ABTS assays were used to evaluate the antioxidant activity of these oils. A good diffusion assay and minimum inhibitory concentrations (MIC) method were used to investigate the antimicrobial activity against pathogenic bacteria and fungi of human interest. Also, the prebiotic activities of oils were tested on three probiotic strains of Lactobacillus to evaluate their role in promoting the growth of beneficial bacteria against the pathogenic E. coli. Furthermore, the haematological effect of these oils was investigated in vitro through measuring their anticoagulant, and Fibrinolytic activity. The results demonstrated that DPPH assay revealed that CPO and WGO exhibited the highest antioxidant activity with IC50 values of 15.2 µg/mL and 18.7 µg/mL, respectively, while BSO showed the least activity with an IC50 of 45.3 µg/mL. Antimicrobial activity, assessed using inhibition zone diameters, showed that CPO had the strongest effect against Staphylococcus aureus with a zone of 22 mm, followed by CSO at 19 mm. In terms of anticoagulant activity, CSO demonstrated the most potent fibrinolytic effect with a clot lysis percentage of 78%, while MO exhibited weaker activity at 35%. Prebiotic testing revealed that individual oils had limited effects on Lactobacillus growth, but a synergistic blend enhanced growth by 25% compared to controls. Overall, this study highlights the diverse health benefits of these oils and their potential as functional food ingredients that could contribute to improved health.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"81"},"PeriodicalIF":3.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12106174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced production and purification of L-asparaginase from Bacillus paralicheniformis AUMC B-516 with potent cytotoxicity against MCF-7 cell lines.","authors":"Abdullah Abobakr Saleh, Hamdy M El-Aref, Azza M Ezzeldin, Rania M Ewida, Osama A M Al-Bedak","doi":"10.1186/s13568-025-01890-w","DOIUrl":"10.1186/s13568-025-01890-w","url":null,"abstract":"<p><p>L-asparaginase is an important enzyme used in the pharmaceutical and food industries. Nowadays, bacterial species represent the main source of microbial synthesis for L-asparaginase. But studies aimed at improving production yields and new methods that use various microbes to expand the scope of application for the generated enzyme are also necessary for industrial manufacturing. This study focused on the isolation of Bacillus paralicheniformis AUMC B-516 and the optimization of L-asparaginase production under submerged fermentation. The enzyme was purified and characterized, followed by an evaluation of its cytotoxic effects against the MCF-7 human breast cancer cell line. The results revealed potent anticancer activity, highlighting the potential application of the purified enzyme in cancer therapeutics. Bacillus paralicheniformis AUMC B-516 was utilized for the biosynthesis of L-asparaginase (116.4 U/mL) after 48 h in the presence of 0.2% glucose and 1.0% L-asparagine at 35 °C and pH 8.0. Two-step chromatography (DEAE-cellulose and Sephacryl S200 HR) achieved a 12-fold purification, resulting in an enzyme specific activity of 4087.6 U/mg. For pure L-asparaginase that contained L-asparagine, the Km and Vmax values were 6.22 × 10^-2 mM and 120.75 µmol/min, respectively. Quantitative assessment of DNA fragmentation in MCF‑7 cells treated with B. paralicheniformis B-516' pure L-asparaginase was performed (22.2 ± 1.36%) and the drug doxorubicin (23.9 ± 0.93%) were significantly greater than those in the negative control cells (8.9 ± 0.83%). MCF-7 cells treated with 1000, 500, 250, 125, 62.5, or 31.25 µg/mL B. paralicheniformis AUMC B-516' pure L-asparaginase showed considerable cytotoxicity, with an IC₅₀ of 49.3 µg/mL. Biochemical analyses revealed significant increases in aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities, while blood glucose, other electrolyte levels, and indicators of renal function remained unchanged. These findings highlight B. paralicheniformis AUMC B-516 as a promising source of L-asparaginase for future biotechnological and pharmaceutical applications.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"80"},"PeriodicalIF":3.5,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: A method for creating in-frame insertions of fluorescent proteins in non-model gram-negative bacteria.","authors":"Darshan Chandramowli, Bart Devreese","doi":"10.1186/s13568-025-01883-9","DOIUrl":"10.1186/s13568-025-01883-9","url":null,"abstract":"","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"78"},"PeriodicalIF":3.5,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromatographic fingerprinting and antibiofilm effect of Ziziphus jujuba fraction on Pseudomonas aeruginosa.","authors":"Mohamed Turkey, Jilan A Nazeam","doi":"10.1186/s13568-025-01886-6","DOIUrl":"10.1186/s13568-025-01886-6","url":null,"abstract":"<p><p>Pseudomonas aeruginosa represents a critical global health threat, particularly affecting immunocompromised individuals, as well as patients with wounds and burn injuries. The increasing prevalence of multidrug-resistant (MDR) P. aeruginosa strains has significantly reduced the efficacy of conventional antimicrobial therapies, underscoring the urgent need for new, effective therapeutic alternatives. Plant-derived secondary metabolites have emerged as promising candidates due to their diverse bioactivities and favorable safety profiles. This study investigated the antimicrobial and anti-virulence potential of purified aqueous fractions of Ziziphus jujuba (ZJ) seeds against MDR P. aeruginosa clinical isolates. LC-ESI-MS/MS-MRM fingerprinting identified 33 compounds, including five predominant phenolics: 3,4-dihydroxybenzoic acid, gallic acid, syringic acid, chlorogenic acid, and ferulic acid. One hundred clinical isolates were evaluated for antibiotic sensitivity and biofilm-forming ability. The ZJ fraction exhibited potent antibacterial activity, with a minimum inhibitory concentration (MIC) of 1.56 mg/ml and significantly inhibited biofilm formation by approximately 70%. Additionally, quantitative real-time PCR showed a marked downregulation the key quorum-sensing genes lasI (45%), rhlI (42%), and rhlR (34%) (p ≤ 0.05). These findings reveal, for the first time, that the aqueous fraction of Z. jujuba seeds not only inhibits bacterial proliferation, but also attenuates biofilm formation and virulence gene expression in MDR-P. aeruginosa. These results highlight the potential of ZJ fraction as a promising plant-based antimicrobial agent. Further in vivo investigations and mechanistic studies are warranted to validate its clinical applicability and therapeutic efficacy.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"79"},"PeriodicalIF":3.5,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}