AMB Express最新文献_第2页

Antiviral activity of selected plant-derived polyphenols against Varicellovirus bovinealpha1 (BoAHV-1). 植物源性多酚对牛水痘病毒1 （BoAHV-1）的抗病毒活性

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-30 DOI: 10.1186/s13568-025-01945-y

Jovana Mitrovic, Verica Aleksic Sabo, Gospava Lazic, Petar Knezevic

{"title":"Antiviral activity of selected plant-derived polyphenols against Varicellovirus bovinealpha1 (BoAHV-1).","authors":"Jovana Mitrovic, Verica Aleksic Sabo, Gospava Lazic, Petar Knezevic","doi":"10.1186/s13568-025-01945-y","DOIUrl":"10.1186/s13568-025-01945-y","url":null,"abstract":"Medicinal plants have been used for centuries in traditional practices to treat a wide range of ailments, including viral infections. Phytochemicals found in these plants represent a promising and largely untapped resource for the development of novel antiviral agents, particularly in light of the limited availability of effective antiviral therapies. This study aimed to evaluate the antiviral potential of five polyphenolic compounds commonly found in medicinal plants: quercetin, rutin, baicalein, (-)-epicatechin, and ethyl gallate against bovine alphaherpesvirus type 1 (BoAHV-1), used as a model for the Orthoherpesviridae family. The cytotoxicity and antiviral activity of the compounds were assessed in vitro using the colorimetric MTT assay. Antiviral effects were quantified based on the concentration required to inhibit 50% of viral replication (IC₅₀). Compounds were tested individually and in combination, with treatments applied before, during, and after viral infection. Expression of the viral gB gene was evaluated using RT-qPCR. Quercetin, rutin, and baicalein exhibited notable antiviral activity, with IC₅₀ values of 16.8 µg mL⁻1, 21.4 µg mL⁻1, and 26.4 µg mL⁻1, respectively, and high selectivity indices (> 15.4, 10.0, and 8.0). In contrast, (-)-epicatechin and ethyl gallate showed no measurable antiviral effect (IC₅₀ > 256 µg mL⁻1). Combinatorial treatments revealed significant synergistic effects. All active compounds reduced gB gene expression, regardless of the timing of treatment. The results support the antiviral potential of certain plant-derived polyphenols, both alone and in synergy, underscoring their promise as candidates for the development of novel antiviral therapies targeting herpesviruses.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"141"},"PeriodicalIF":3.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a newly isolated biosurfactant fengycin produced by Heyndrickxia coagulans strain. 新分离的Heyndrickxia凝固菌制备的生物表面活性剂凤霉素的表征。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-30 DOI: 10.1186/s13568-025-01942-1

Eva Styková, Zdenka Bedlovičová, Natália Šurín Hudáková, Viera Karaffová, Katarína Kalocsaiová, Mangesh Bhide

{"title":"Characterization of a newly isolated biosurfactant fengycin produced by Heyndrickxia coagulans strain.","authors":"Eva Styková, Zdenka Bedlovičová, Natália Šurín Hudáková, Viera Karaffová, Katarína Kalocsaiová, Mangesh Bhide","doi":"10.1186/s13568-025-01942-1","DOIUrl":"10.1186/s13568-025-01942-1","url":null,"abstract":"One of the major global concerns in human and veterinary medicine at present is the antimicrobial resistance to conventional antibiotics. Natural products from microbial sources appear to be the most favorable alternative to current antibiotics. Biosurfactants (BSs) are surface-active compounds synthesised by a wide variety of microorganisms. Fengycin is an important member of the lipopeptide family, with a wide range of applications in different industries and a broad spectrum of antimicrobial activity against pathogenic microbes. The production of fengycin has been detected in various strains of Bacillus spp., but to our knowledge, not in Heyndrickxia coagulans. Strain H. coagulans (formerly known as B. coagulans) Biocenol™ 9FT27 CCM 9014, which exhibited surfactant activity as assessed by oil spreading assay, was characterised using the 16S rRNA sequencing method. PCR screening detected the presence of fenD, indicating the production of the lipopeptide biosurfactant fengycin. The results of UHPLC-DAD, NMR and MALDI-TOF/MS analysis confirmed the production of fengycin by H. coagulans 9FT27. BS was found to significantly (P < 0.0001) inhibit the biofilm formation of Staphylococcus aureus CCM 4223 and MRSA (methicillin-resistant Staphylococcus aureus) at concentrations ranging from 15 to 0.2 mg/mL. Analysis of qRT-PCR results revealed reduced expression of the srtA, gyrB, clfB and icaADB operon genes, which are associated with biofilm formation. Our results indicate the potential of fengycin in the control of biofilm-related infections, especially those caused by antibiotic-resistant S. aureus strains.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"139"},"PeriodicalIF":3.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and probiotic potential of thermotolerant lactic acid bacteria from silage for ensiling crop residues. 青贮作物残茬青贮耐热乳酸菌特性及益生菌潜力研究。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-30 DOI: 10.1186/s13568-025-01941-2

Rashika Srivastava, Bilal Chamadia, Pradip V Behare, Komal Chauhan, Sachin Kumar, Goutam Mondal, Nitin Tyagi

{"title":"Characterization and probiotic potential of thermotolerant lactic acid bacteria from silage for ensiling crop residues.","authors":"Rashika Srivastava, Bilal Chamadia, Pradip V Behare, Komal Chauhan, Sachin Kumar, Goutam Mondal, Nitin Tyagi","doi":"10.1186/s13568-025-01941-2","DOIUrl":"10.1186/s13568-025-01941-2","url":null,"abstract":"Paddy straw, though abundantly available in India, is often burnt in fields, despite its potential as valuable feed resource for livestock. Ensiling provides a practical solution for its utilization, but challenges like low moisture, high fibre content, and unfavourable climatic conditions limit its effectiveness. In recent times, microbial-based approaches, particularly involving lactic acid bacteria (LAB), have shown promise in improving silage quality. However, suitable thermotolerant LAB strains that can perform under Indian field conditions remain unexplored. To address this, 45 Gram-positive, catalase-negative, and vancomycin-resistant LAB were isolated from naturally fermented silage collected from hot and humid regions of the country. Genus-specific 16S rRNA PCR confirmed all isolates as Lactobacilli and out of these, 35 were acid-tolerant, 26 bile-tolerant, and 12 could grow at 47-50 °C. RSB7 showed the maximum growth at elevated temperatures. Among thermotolerant cultures, six each were identified as homofermentative and heterofermentative, and were preserved in the National Collection of Dairy Cultures. RSB13 showed the highest hydrophobicity. RSB2 had superior co-aggregation and antioxidant activity, while RSB9 exhibited strong autoaggregation, ferulic acid esterase activity, and in vitro straw degradability. All isolates were non-hemolytic, non-mucinolytic, and antibiotic-sensitive. These thermotolerant Lactobacillus spp. showed good potential as safe and functional silage inoculants suitable for tropical Indian conditions.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"140"},"PeriodicalIF":3.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-grade green coffee with Bacillus amyloliquefaciens NY124 protease: extraction, purification, and functional synergy of chlorogenic acid and trigonelline. 含解淀粉芽孢杆菌NY124蛋白酶的低级生咖啡：绿原酸和葫芦巴碱的提取、纯化和功能协同。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-29 DOI: 10.1186/s13568-025-01920-7

Ae Eun Im, Deepti Bharti, Geonwoo Lim, Yunha Bak, Jung Choi, Kwang-Yeol Yang, Namhyeon Park, Seung-Hee Nam

{"title":"Low-grade green coffee with Bacillus amyloliquefaciens NY124 protease: extraction, purification, and functional synergy of chlorogenic acid and trigonelline.","authors":"Ae Eun Im, Deepti Bharti, Geonwoo Lim, Yunha Bak, Jung Choi, Kwang-Yeol Yang, Namhyeon Park, Seung-Hee Nam","doi":"10.1186/s13568-025-01920-7","DOIUrl":"10.1186/s13568-025-01920-7","url":null,"abstract":"Defective green coffee beans contain bioactive compounds like chlorogenic acid (CGA) and trigonelline, but high caffeine limits applications. For cost-effective, high-purity industrial use of functional ingredients, this study extracted and purified CGA and trigonelline from low-grade green coffee beans using bacterial enzyme and open column chromatography. Steam pretreatment enhanced the CGA content reaching 35 g/kg of dry weight. Among six strains, Bacillus amyloliquefaciens NY124 was selected for its superior CGA and trigonelline extraction. Its enzyme, a 42 kDa protease, was purified and confirmed by SDS-PAGE and zymography. CGA production doubled with 1.88 U/mL NY124 protease, 7.87% (w/v) green coffee, and 6.85 h via response surface methodology. Diaion HP-20 resins removed 89% of caffeine while silica column chromatography yielded caffeine-free CGA, and trigonelline both achieving high (> 95%) purity. The decaffeinated isolate (Decaf), comprising CGA and trigonelline, exhibited greater functional activity than either fraction alone, highlighting their synergistic effect. Decaf exhibited tenfold higher antioxidant activity than control, along with 52.97% α-glucosidase inhibition (five-fold over control). Decaf isolate demonstrated anti-obesity effects by significantly reducing lipid accumulation by 37% and enhancing lipolysis, evidenced by an 89% increase in free glycerol release compared to the non-treated 3T3-L1 adipocytes.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"138"},"PeriodicalIF":3.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12480324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145190714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phage receptor binding protein and Fc fragment fusion enhances phagocytosis of Y. enterocolitica. 噬菌体受体结合蛋白与Fc片段融合增强小肠结肠炎菌的吞噬作用。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-26 DOI: 10.1186/s13568-025-01948-9

Karolina Filik-Matyjaszczyk, Irwin Matyjaszczyk, Marzena Ciesielska, Bożena Szermer-Olearnik, Krzysztof Mikołajczyk, Andrzej Gamian

{"title":"Phage receptor binding protein and Fc fragment fusion enhances phagocytosis of Y. enterocolitica.","authors":"Karolina Filik-Matyjaszczyk, Irwin Matyjaszczyk, Marzena Ciesielska, Bożena Szermer-Olearnik, Krzysztof Mikołajczyk, Andrzej Gamian","doi":"10.1186/s13568-025-01948-9","DOIUrl":"10.1186/s13568-025-01948-9","url":null,"abstract":"Bacteriophage receptor binding proteins (RBPs) belong to a group of proteins that are components of the bacteriophage tail. RBPs are mainly responsible for recognizing receptors on the bacterial host surface and enabling infection and subsequent progeny phage multiplication. Our previous research suggested that recombinant tail fiber gp17 (TFPgp17) protein from Yersinia enterocolitica phage φYeO3-12 recognizes serotype O:3 with high specificity and could be used as a diagnostic tool. In this paper, we study the possibility of using modified TFPgp17 in fusion with IgG1 Fc immunoglobulin fragment (Fc_TFPgp17) to improve the efficacy of pathogen recognition by phagocytic cells, opsonization of bacterial cells, and then phagocytosis by the THP-1 macrophages and the HL-60 neutrophils. We demonstrate that the Fc_TFPgp17 protein can bind both bacterial and phagocytic cells, due to the presence of the RBP domain and the IgG1 Fc fragment, respectively. Additionally, it contributes to the better recognition of bacterial cells and their subsequent phagocytosis by phagocytes. Additionally, we proved that glycosylation of Fc_TFPgp17 which occurred during protein production, harms the ability to recognize Y. enterocolitica O:3. Fc_TFPgp17 is a bispecific protein that possesses two biological functions: (I) recognized Y. enterocolitica O:3 cells via phage RBP (TFPgp17) and (II) recognized phagocytic cells via Fc-fragment IgG1 immunoglobulin. In this work, we demonstrated that the recombined bispecific protein facilitates more effective recognition and opsonization of the pathogenic bacterial cell, leading to its subsequent phagocytosis.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"135"},"PeriodicalIF":3.7,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput profiling of fungal diversity and physicochemical dynamics in strong-aroma Daqu. 强香气大曲真菌多样性及理化动态的高通量分析。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-26 DOI: 10.1186/s13568-025-01944-z

Lan Chaohua, Zhou Wei, Zhou Xiaoli, Guo Zhengxiang, Peng Tao, Jiang Renjie, Wang Lingxiang, Cheng Xinkai, Ye Beiling, He Chaojiu

引用次数: 0

Correction: Antimicrobial and molluscicidal activities of Egyptian soil-derived Streptomyces rochei. 更正：埃及土源罗氏链霉菌的抗菌和杀软体活性。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-26 DOI: 10.1186/s13568-025-01959-6

Nora Elfeky, Aya Abd Elsalam, Sabha El-Sabbagh, Asmaa Abdel-Motleb

引用次数: 0

Sexual behavior influences the abundance of Prevotellaceae family in men who have sex with men. 性行为影响男男性行为者普氏菌科菌群的丰度。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-26 DOI: 10.1186/s13568-025-01957-8

Haijiao Zeng, Jiaming Jiang, Kangjie Li, Cong Zhang, Xiaohua Zhong, Pinyi Chen, Tian Liu, Yingcheng Liu, Wenlong Li, Biao Xie, Xiaoni Zhong

引用次数: 0

Propeptide-mediated enhancement of hyperthermophilic subtilisin-like protease expression in Escherichia coli. 前肽介导的大肠杆菌中嗜热枯草杆菌样蛋白酶表达的增强。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-26 DOI: 10.1186/s13568-025-01952-z

Ryo Uehara, Shuto Nishizaki, Hiroshi Amesaka, Kazufumi Takano, Hiroyoshi Matsumura, Shun-Ichi Tanaka

引用次数: 0

Repurposing rupatadine as topical treatment against methicillin-resistant Staphylococcus aureus. 重新利用鲁帕他定作为局部治疗抗甲氧西林金黄色葡萄球菌。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-23 DOI: 10.1186/s13568-025-01947-w

Asmaa Farag, Dalia El-Damasy, Dalia Soliman, Galal Yahya, Kareem Ibrahim

{"title":"Repurposing rupatadine as topical treatment against methicillin-resistant Staphylococcus aureus.","authors":"Asmaa Farag, Dalia El-Damasy, Dalia Soliman, Galal Yahya, Kareem Ibrahim","doi":"10.1186/s13568-025-01947-w","DOIUrl":"10.1186/s13568-025-01947-w","url":null,"abstract":"This study evaluates the potential of repurposed non-antibiotic drugs as possible treatments for methicillin-resistant Staphylococcus aureus (MRSA) skin infection, through a comprehensive approach combining in silico, in vitro, and in vivo methodologies. From 198 protein targets previously identified through multi-omics analysis, 10 essential, druggable, broad-spectrum targets were selected. Three promising non-antibiotic ligands (rupatadine, orlistat, and citric acid) were evaluated through minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), molecular docking, cytotoxicity testing, and a murine model of MRSA skin infection. Rupatadine, orlistat, and citric acid showed MICs of 0.031 mg/mL, 1.5 mg/mL, and 2.5 mg/mL, respectively. Rupatadine demonstrated the most potent anti-MRSA activity, with molecular docking revealing favorable binding energy (- 3.45 kcal/mol) to Aminoacyl transferase (FemA). Serial passage experiments over eight passages showed no resistance development against rupatadine in one MRSA strain and only twofold increase in another, compared to 8-13 fold increases observed with conventional antibiotics. Checkerboard assays revealed synergistic effects between rupatadine and β-lactam antibiotics (cefazolin and cefotaxime, FICI = 0.3). FemA is essential in cell wall biosynthesis and represents an essential target for anti-MRSA agents. Functional enrichment and pathway network analyses revealed significant disruption of biological processes governing peptidoglycan biosynthesis and cell wall biogenesis, which indicates the possibility of rupatadine compromising S. aureus cell wall integrity through interference with peptidoglycan assembly pathways, supporting its potential as a repurposed anti-MRSA agent. Rupatadine showed favorable tolerance on human skin fibroblast cells (IC50: 1150 µg/mL), and significantly reduced bacterial count in a murine model.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"132"},"PeriodicalIF":3.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0