AMB Express最新文献_第2页

Correction: Antimicrobial and molluscicidal activities of Egyptian soil-derived Streptomyces rochei. 更正：埃及土源罗氏链霉菌的抗菌和杀软体活性。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-26 DOI: 10.1186/s13568-025-01959-6

Nora Elfeky, Aya Abd Elsalam, Sabha El-Sabbagh, Asmaa Abdel-Motleb

引用次数: 0

Sexual behavior influences the abundance of Prevotellaceae family in men who have sex with men. 性行为影响男男性行为者普氏菌科菌群的丰度。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-26 DOI: 10.1186/s13568-025-01957-8

Haijiao Zeng, Jiaming Jiang, Kangjie Li, Cong Zhang, Xiaohua Zhong, Pinyi Chen, Tian Liu, Yingcheng Liu, Wenlong Li, Biao Xie, Xiaoni Zhong

引用次数: 0

Propeptide-mediated enhancement of hyperthermophilic subtilisin-like protease expression in Escherichia coli. 前肽介导的大肠杆菌中嗜热枯草杆菌样蛋白酶表达的增强。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-26 DOI: 10.1186/s13568-025-01952-z

Ryo Uehara, Shuto Nishizaki, Hiroshi Amesaka, Kazufumi Takano, Hiroyoshi Matsumura, Shun-Ichi Tanaka

引用次数: 0

Repurposing rupatadine as topical treatment against methicillin-resistant Staphylococcus aureus. 重新利用鲁帕他定作为局部治疗抗甲氧西林金黄色葡萄球菌。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-23 DOI: 10.1186/s13568-025-01947-w

Asmaa Farag, Dalia El-Damasy, Dalia Soliman, Galal Yahya, Kareem Ibrahim

{"title":"Repurposing rupatadine as topical treatment against methicillin-resistant Staphylococcus aureus.","authors":"Asmaa Farag, Dalia El-Damasy, Dalia Soliman, Galal Yahya, Kareem Ibrahim","doi":"10.1186/s13568-025-01947-w","DOIUrl":"10.1186/s13568-025-01947-w","url":null,"abstract":"This study evaluates the potential of repurposed non-antibiotic drugs as possible treatments for methicillin-resistant Staphylococcus aureus (MRSA) skin infection, through a comprehensive approach combining in silico, in vitro, and in vivo methodologies. From 198 protein targets previously identified through multi-omics analysis, 10 essential, druggable, broad-spectrum targets were selected. Three promising non-antibiotic ligands (rupatadine, orlistat, and citric acid) were evaluated through minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), molecular docking, cytotoxicity testing, and a murine model of MRSA skin infection. Rupatadine, orlistat, and citric acid showed MICs of 0.031 mg/mL, 1.5 mg/mL, and 2.5 mg/mL, respectively. Rupatadine demonstrated the most potent anti-MRSA activity, with molecular docking revealing favorable binding energy (- 3.45 kcal/mol) to Aminoacyl transferase (FemA). Serial passage experiments over eight passages showed no resistance development against rupatadine in one MRSA strain and only twofold increase in another, compared to 8-13 fold increases observed with conventional antibiotics. Checkerboard assays revealed synergistic effects between rupatadine and β-lactam antibiotics (cefazolin and cefotaxime, FICI = 0.3). FemA is essential in cell wall biosynthesis and represents an essential target for anti-MRSA agents. Functional enrichment and pathway network analyses revealed significant disruption of biological processes governing peptidoglycan biosynthesis and cell wall biogenesis, which indicates the possibility of rupatadine compromising S. aureus cell wall integrity through interference with peptidoglycan assembly pathways, supporting its potential as a repurposed anti-MRSA agent. Rupatadine showed favorable tolerance on human skin fibroblast cells (IC50: 1150 µg/mL), and significantly reduced bacterial count in a murine model.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"132"},"PeriodicalIF":3.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Deciphering the Withania somnifera alkaloids potential for cure of neurodegenerative disease: an in-silico study. 更正：破译苦藤生物碱治疗神经退行性疾病的潜力：一项计算机研究。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-17 DOI: 10.1186/s13568-025-01939-w

Ruili Chen, Dongdong Zhang, Anis Ahmad Chaudhary, Salah-Ud-Din Khan, Saeed Vohra, Yusra Vohra, Nini Li, Shahanavaj Khan

引用次数: 0

Antibacterial, antibiofilm and cytotoxic activity of synthesized metal-incorporated mesoporous silica nanoparticles. 合成金属掺入介孔二氧化硅纳米颗粒的抗菌、抗生物膜和细胞毒活性。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-17 DOI: 10.1186/s13568-025-01938-x

Suzan Shehata, Yomna N Elkholy, Mai S A Hussien, Ibrahim S Yahia, Khaled M Aboshanab

{"title":"Antibacterial, antibiofilm and cytotoxic activity of synthesized metal-incorporated mesoporous silica nanoparticles.","authors":"Suzan Shehata, Yomna N Elkholy, Mai S A Hussien, Ibrahim S Yahia, Khaled M Aboshanab","doi":"10.1186/s13568-025-01938-x","DOIUrl":"10.1186/s13568-025-01938-x","url":null,"abstract":"Antibiotic misuse has triggered a global health crisis due to the rise of antimicrobial-resistant microorganisms. Recent studies suggest that nanoparticles (NPs), particularly silver nanoparticles, may provide a solution. However, their toxicity necessitates the development of novel carriers to enhance targeting while minimizing cytotoxicity. This study aimed to synthesize, characterize mesoporous silica nanoparticles (MSN) incorporated with silver alone or combined with zinc, and evaluate their antibacterial, antibiofilm, and cytotoxic properties. Characterization through X-ray diffraction (XRD) and scanning electron microscopy (SEM), revealed particle sizes between 413 and 560 nm. Antibacterial activity was assessed via the broth-dilution method against Staphylococcus aureus ATCC 25,923, Escherichia coli ATCC 25,922, Methicillin-resistant Staphylococcus aureus (MRSA) ATCC 33,591, and five multidrug-resistant pathogens (MDR). Two types of silver-incorporated mesoporous silica nanoparticles were synthesized: Ag/MCM-48 (10 mmol Ag) and Ag/Zn/MCM-48 (10 mmol Ag + 10 mmol Zn). The minimum inhibitory and minimum bactericidal concentrations ranged from 7.8 to 31.25 and 7.8 to 62.5 µg/mL, respectively. Ag/MCM-48 NPs exhibited 78% antibiofilm activity against MRSA ATCC 33,591, while Ag/Zn/MCM-48 NPs displayed up to 90% antibiofilm activity against MDR bacteria. Cytotoxicity assays revealed promising results, with CC50 values of 169.16 ± 6.43 µg/mL and 19.95 ± 0.63 µg/mL for Ag/Zn/MCM-48 NPs and Ag/MCM-48 NPs against Vero cell line, respectively. The IC50 values of Ag/Zn/MCM-48 and Ag/MCM-48 NPs were 62.17 ± 2.15 µg/mL and 3.58 ± 0.12 µg/mL against Caco-2 cell line, respectively. Accordingly, our synthesized silver/zinc incorporated mesoporous silica NPs present a safer antibacterial and antibiofilm agent with lower cytotoxicity than silver NPs, making them a promising alternative for combating MDR bacteria.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"130"},"PeriodicalIF":3.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12443651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Comparative evaluation of live attenuated and killed tachyzoites as vaccine candidates for toxoplasmosis. 更正：对减毒活速虫和灭活速虫作为弓形虫病候选疫苗的比较评价。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-09-11 DOI: 10.1186/s13568-025-01936-z

Eman E El Shanawany, Eman H Abdel-Rahman, Waleed A Nemr, Soad E Hassan, Noha M F Hassan, Hassan M Desouky, Rabab Zalat, Amany Ebrahim Nofal, Raafat M Shaapan, Salwa Sami Younis

引用次数: 0

Synbiotic microencapsulation of Lactiplantibacillus plantarum-lentinan for enhanced growth in broilers. 植物乳杆菌-香菇多糖合成微囊化促进肉鸡生长。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-08-31 DOI: 10.1186/s13568-025-01929-y

Liyang Guo, Lixia Yang, Kun Wang, Weiqi Liu, Shun Wang, Yongjie Zhang, Rui Li, Zhiyong Wu, Chunli Chen

引用次数: 0

Genome-wide analysis of Burkholderia for the management of antimicrobial-resistant in cystic fibrosis patients. 伯克氏菌全基因组分析对囊性纤维化患者抗菌素耐药的管理。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-08-26 DOI: 10.1186/s13568-025-01926-1

Vijayavidhya Magizhvannan, Shanthi Veerappapillai

{"title":"Genome-wide analysis of Burkholderia for the management of antimicrobial-resistant in cystic fibrosis patients.","authors":"Vijayavidhya Magizhvannan, Shanthi Veerappapillai","doi":"10.1186/s13568-025-01926-1","DOIUrl":"10.1186/s13568-025-01926-1","url":null,"abstract":"Burkholderia is a significant pathogen that causes disease burden across the globe. In particular, Burkholderia cenocepacia and Burkholderia multivorans are the predominant isolates that infect people with cystic fibrosis (CF) and cause hospital-acquired infections. Understanding antimicrobial resistance and virulent factors among these species is of great significance for addressing this growing resistance burden. Initially, we retrieved 75 complete genome sequences of B. cenocepacia and B. multivorans from NCBI database and analysed them for antimicrobial resistance (AMR) and virulent factors. This yielded 368 antimicrobial resistance genes and 202 virulent factors after removing the duplicates. Further, a comprehensive interaction network was constructed using STRING, which was visualized and analysed using Cytoscape. Through cytoHubba and MCODE analysis, eight key hub genes FliF, FliG, FliM, FliS, FlgB, FlgC, FlgD and FlgK were identified. Additionally, a non-homology analysis was conducted to ensure that the key nodes do not exhibit similarity with the human genome and gut microbiota. Functional enrichment analysis revealed their significant role in the flagellar assembly pathway, particularly in bacterial motility, colonization and biofilm formation. Notably, seven hub genes were enriched in bacterial-type flagellum-dependent cell motility pathway and cellular localization. It is worth noting that 17,967 phytochemicals were exploited to identify the potent hit compounds against each of the identified hub genes. Interestingly, the hit molecules were found to form several key interactions with the targets, indicating their potential as promising therapeutic agents for combating AMR. Overall, the identified hub genes and their potent inhibitors present compelling targets for novel antimicrobial therapies in CF, underscoring the need for future experimental validation.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"127"},"PeriodicalIF":3.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treponema pallidum inhibits CD4+ T-cell proliferation through METAP2: insights from Mendelian randomization analysis. 梅毒螺旋体通过METAP2抑制CD4+ t细胞增殖：来自孟德尔随机化分析的见解

IF 3.7 3区生物学

AMB Express Pub Date : 2025-08-25 DOI: 10.1186/s13568-025-01940-3

Zhaoping Liu, Xiaohong Zhang, Ting Lin, Xuan Ding, Han Yu, Jiangchen Yao, Ke Gao, Yimou Wu, Feijun Zhao

{"title":"Treponema pallidum inhibits CD4+ T-cell proliferation through METAP2: insights from Mendelian randomization analysis.","authors":"Zhaoping Liu, Xiaohong Zhang, Ting Lin, Xuan Ding, Han Yu, Jiangchen Yao, Ke Gao, Yimou Wu, Feijun Zhao","doi":"10.1186/s13568-025-01940-3","DOIUrl":"10.1186/s13568-025-01940-3","url":null,"abstract":"Neurosyphilis (NS) is a chronic central nervous system infection caused by Treponema pallidum. Owing to its diverse clinical manifestations and the limited sensitivity of current diagnostic methods, NS is difficult to diagnose. Understanding the molecular mechanisms of NS and identifying reliable biomarkers are essential for improving diagnostic and therapeutic strategies. This study employed Mendelian randomization (MR) analysis to explore the causal relationships among protein ratio quantitative trait loci (rQTLs), cerebrospinal fluid (CSF) metabolites, and syphilis risk at various stages. The results revealed that several rQTLs, including CD46/TNFRSF14 and TBC1D23/TBC1D5, were closely associated with syphilis risk, whereas others, such as BANK1/HEXIM1 and GOPC/HEXIM1, exhibited protective effects. Mediation analysis further identified key CSF metabolites, such as N-acetyltaurine and bilirubin, as important mediators linking rQTLs and syphilis progression. Through integrated analysis of cis-proteins from rQTLs and transcriptomic data from CD4 + T-cells of NS patients, METAP2 was identified as a key biomarker in NS, with the potential mechanisms elucidated. Importantly, T. pallidum may inhibit CD4 + T-cell proliferation by modulating METAP2, thereby accelerating disease progression. These findings offer new insights into the pathogenesis of NS and highlight METAP2 as a potential biomarker, laying a foundation for improving diagnostic and therapeutic strategies.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"126"},"PeriodicalIF":3.7,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12379665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0