AMB Express最新文献_第2页

Mediators linking gut microbiota and sporadic Creutzfeldt-Jakob disease: a Mendelian randomization study. 连接肠道微生物群和散发性克雅氏病的介质：一项孟德尔随机研究

IF 3.7 3区生物学

AMB Express Pub Date : 2025-08-18 DOI: 10.1186/s13568-025-01932-3

Jie Shao, Tengfei Su, Jinyan Wang, Xiang Yin, Yue Lang, Yuxin Fu, Li Cui

{"title":"Mediators linking gut microbiota and sporadic Creutzfeldt-Jakob disease: a Mendelian randomization study.","authors":"Jie Shao, Tengfei Su, Jinyan Wang, Xiang Yin, Yue Lang, Yuxin Fu, Li Cui","doi":"10.1186/s13568-025-01932-3","DOIUrl":"10.1186/s13568-025-01932-3","url":null,"abstract":"While gut microbiome associations with sporadic Creutzfeldt-Jakob disease (sCJD) are recognized, causal mechanisms and mediation via cerebrospinal fluid (CSF) metabolites remain unestablished. Using bidirectional Mendelian randomization (MR) with mediation analysis and integrating genome-wide association study (GWAS) summary statistics from gut microbiota (composition in the FINRISK 2002 prospective cohort, n = 5,959), CSF metabolites (from the Wisconsin Alzheimer's Disease Research Center Registry and Wisconsin Registry for Alzheimer's Prevention, n = 291), and sCJD case-control data (5,208 cases vs. 511,675 controls), we identified five microbial taxa influencing sCJD risk. Protective effects were observed for the family Atopobiaceae [odds ratio (OR) = 0.527, 95% confidence interval (CI) = 0.321-0.864, P = 0.011], the species Enterococcus faecalis (OR = 0.647, 95% CI = 0.427-0.980, P = 0.040), and the genus Lactobacillus (group B) (OR = 0.768, 95% CI = 0.602-0.981, P = 0.035). Conversely, the species Bacteroides eggerthii (OR = 1.228, 95% CI = 1.027-1.468, P = 0.025) and the order Chloroflexales (OR = 3.455, 95% CI = 1.214-9.835, P = 0.020) were pathogenic. Mediation analysis revealed that S-methylcysteine mediates 8.8% of the effect of order Chloroflexales on sCJD risk, establishing it as a significant biological mediator in this pathogenic pathway. These findings provide novel biomarkers for early sCJD risk stratification, identify the family Atopobiaceae, the species Enterococcus faecalis, and the genus Lactobacillus (group B) as probiotic candidates for primary prevention, reveal S-methylcysteine pathway modulation as therapeutic entry points, and establish mechanistic foundations for disrupting gut-CSF transmission in prion diseases.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"120"},"PeriodicalIF":3.7,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12360999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic potential of recombinant IL-22BP in psoriasis: suppression of IL-22/STAT3 signaling in mice. 重组IL-22BP对银屑病的治疗潜力：抑制小鼠IL-22/STAT3信号

IF 3.7 3区生物学

AMB Express Pub Date : 2025-08-18 DOI: 10.1186/s13568-025-01931-4

Xiuze Chen, Tao Zeng, Fang Fang, Qinyu Tian, Yingying Li, Wenlin Zhou, Binqian Gong, Na Tian, Jizhou Zhang, Yechen Xiao

{"title":"Therapeutic potential of recombinant IL-22BP in psoriasis: suppression of IL-22/STAT3 signaling in mice.","authors":"Xiuze Chen, Tao Zeng, Fang Fang, Qinyu Tian, Yingying Li, Wenlin Zhou, Binqian Gong, Na Tian, Jizhou Zhang, Yechen Xiao","doi":"10.1186/s13568-025-01931-4","DOIUrl":"10.1186/s13568-025-01931-4","url":null,"abstract":"Psoriasis is a persistent immune-mediated inflammatory disorder that adversely affects the skin. Interleukin-22 (IL-22) is integral to the development and pathophysiology of psoriasis, and targeting IL-22 may serve as a promising therapeutic approach for treating the condition. IL-22 binding protein (IL-22BP) exhibits a binding affinity for IL-22 that far surpasses that of IL-22RA1 and functions as a natural antagonist of IL-22. Traditional IL-22BP production methods predominantly rely on eukaryotic animal cell expression systems, which generally require complex processes, resulting in low yield and high production costs. This study reports the expression of long-acting IL-22BP with a high yield and purity over 90% in Escherichia coli by fusion with the albumin-binding structural domain ABD. The biological functions of rhIL-22BP-ABD were assessed utilizing cell lines and a murine model. Our findings indicated that rhIL-22BP-ABD successfully suppressed IL-22-induced proliferation of HaCaT cells in vitro and alleviated imiquimod-induced psoriasis inflammation in mice. Furthermore, rhIL-22BP-ABD can effectively inhibit the signaling of its downstream signaling pathway STAT3 and the associated inflammatory factors by binding to IL-22, which is beneficial to the recovery of psoriasis. These findings provide a basis for forthcoming extensive studies on the rhIL-22BP-ABD protein for industrial manufacturing and pharmaceutical development.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"121"},"PeriodicalIF":3.7,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12361007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial activity of fungus comb extracts from Senegalese fungus-farming termites. 塞内加尔养殖白蚁菌冠提取物的抑菌活性。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-08-13 DOI: 10.1186/s13568-025-01917-2

Mapenda Gaye, Amira Zhor Rim Zinai, Nicholas Armstrong, Gaetan Herbette, Valérie Monnier, Hubert Bassene, Georges Diatta, Masse Sambou, Cheikh Sokhna, Didier Raoult, Florence Fenollar, Oleg Mediannikov

{"title":"Antibacterial activity of fungus comb extracts from Senegalese fungus-farming termites.","authors":"Mapenda Gaye, Amira Zhor Rim Zinai, Nicholas Armstrong, Gaetan Herbette, Valérie Monnier, Hubert Bassene, Georges Diatta, Masse Sambou, Cheikh Sokhna, Didier Raoult, Florence Fenollar, Oleg Mediannikov","doi":"10.1186/s13568-025-01917-2","DOIUrl":"10.1186/s13568-025-01917-2","url":null,"abstract":"Fungus-farming termites (Macrotermitinae), predominantly found in Africa, are eusocial insects with significant ecological roles. Historically, they have been valued in traditional medicine, human diets, and livestock feed. These termites share a long-standing symbiotic relationship with Termitomyces fungi, which has evolved over millions of years and is critical to their survival and ecological impact. This mutualism promotes a unique monoculture of Termitomyces in the fungus comb while suppressing fungal and bacterial antagonists, likely due to the comb's structural or chemical properties, sparking interest among researchers. In this study, we conducted an extensive examination of 11 fungus combs associated with five termite species collected in Senegal. Our analysis revealed significant antibacterial properties in the crude extracts of the combs, notably against multidrug-resistant strains. Chemical analyses led to the identification of dicrotalic acid (Meglutol) in the active fractions of two combs from agricultural areas. This compound, likely of plant origin, suggests a link between termite feeding habits and the antimicrobial potential of the combs. Although the exact bioactive compounds responsible for the antimicrobial activity have not yet been fully identified, the presence of various metabolites may explain the maintenance of Termitomyces monocultures and the suppression of pathogens. This also illustrates the complex ecological relationship between Termitomyces and termites, which may work together to produce natural bioactive compounds that suppress pathogens.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"117"},"PeriodicalIF":3.7,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inflammation biomarkers mediate causal inference of the effect of skin microbiota on the risk of allergic diseases. 炎症生物标志物介导皮肤微生物群对过敏性疾病风险影响的因果推断。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-08-12 DOI: 10.1186/s13568-025-01924-3

Yuting Zhang, Yanjuan Wu, Xiaofen Su, Qiming Gan, Yutong Ding, Jingcun Wang, Xinni Wang, Nuofu Zhang, Kang Wu

{"title":"Inflammation biomarkers mediate causal inference of the effect of skin microbiota on the risk of allergic diseases.","authors":"Yuting Zhang, Yanjuan Wu, Xiaofen Su, Qiming Gan, Yutong Ding, Jingcun Wang, Xinni Wang, Nuofu Zhang, Kang Wu","doi":"10.1186/s13568-025-01924-3","DOIUrl":"10.1186/s13568-025-01924-3","url":null,"abstract":"Alterations in skin microbiota composition have been linked to allergic diseases, but the causal relationship remains unclear. To investigate the causal relationship between skin microbiota, allergic diseases, and inflammation biomarkers using Mendelian randomization (MR). We integrated summary statistics from genome-wide association studies (GWAS) of skin microbiota inflammation biomarkers, and seven allergic diseases. Inverse variance weighting (IVW) served as the primary statistical method, with supplementary analyses using MR-Egger regression, weighted median, and Weighted mode. Sensitivity analyses, including Cochran's Q test, MR-Egger intercept test and MR-PRESSO outlier detection, were conducted to validate and stabilize our findings. Two-step MR analyses were performed to identify potential mediating inflammation biomarkers between skin microbiota and allergic diseases.We identified 43 significant causal relationships between the skin microbiota and seven allergic diseases: allergic disease as a whole, asthma (adult, pediatric, allergic), allergic conjunctivitis, allergic rhinitis, atopic dermatitis, allergic urticaria and eczema, which included 20 protective and 23 risk causal relationships, respectively. Mediation analysis showed that specific biomarkers, such as C-C motif chemokine 19 and CD40L receptor levels, Interleukin-18 and TNF-β mediated these associations. This MR study provides robust evidence supporting causal relationships between specific skin microbiota taxa and allergic diseases, as well as potential mediating roles of inflammation biomarkers.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"116"},"PeriodicalIF":3.7,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12343437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Demographic drivers of gut microbiome diversity. 肠道微生物群多样性的人口驱动因素。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-08-08 DOI: 10.1186/s13568-025-01921-6

Hannaneh Kabir, Matilda Holtz, Justine Choueiri, Mohammad Reza Kaazempur Mofrad

{"title":"Demographic drivers of gut microbiome diversity.","authors":"Hannaneh Kabir, Matilda Holtz, Justine Choueiri, Mohammad Reza Kaazempur Mofrad","doi":"10.1186/s13568-025-01921-6","DOIUrl":"10.1186/s13568-025-01921-6","url":null,"abstract":"The gut microbiome plays a central role in orchestrating metabolic, immune, and neurological functions essential for human health. While extensive research has explored the effects of diseases and pathological conditions on gut microbiome composition, the influence of demographic factors remains underexplored, limiting our understanding of microbiome variations in disease states. This study addresses this gap by investigating the impact of demographic variables, including age, sex, and geography, on gut microbiome diversity in healthy individuals. Using the American Gut Project's extensive dataset and the QIIME2 bioinformatics pipeline, we conducted a comprehensive analysis of microbial profiles across diverse demographic groups. Our results revealed significant age-related shifts in microbial richness and composition, and geographic location strongly influenced phylogenetic diversity. In contrast, sex exhibited limited impact on microbial diversity within healthy BMI ranges. These findings highlight the critical role of demographic factors in shaping gut microbiome diversity, providing a foundational framework to better contextualize disease-related microbiome variations and advance personalized healthcare approaches.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"115"},"PeriodicalIF":3.7,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12334389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into the biological activities of Fagonia Arabica L. and its phytochemical constituents. 阿拉比卡Fagonia L.的生物活性及其植物化学成分研究。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-08-01 DOI: 10.1186/s13568-025-01918-1

Nashaat N Mahmoud, Alsayed E Mekky, Esawy Mahmoud, Mayada M El-Azab, Muhammad I Haggag

{"title":"Insight into the biological activities of Fagonia Arabica L. and its phytochemical constituents.","authors":"Nashaat N Mahmoud, Alsayed E Mekky, Esawy Mahmoud, Mayada M El-Azab, Muhammad I Haggag","doi":"10.1186/s13568-025-01918-1","DOIUrl":"10.1186/s13568-025-01918-1","url":null,"abstract":"In Egypt, the genus Fagonia is found in all the deserts of Egypt and the Sinai Peninsula. It is widely used to treat many diseases as a home remedy without sufficient scientific studies, especially Fagonia arabica L. Therefore, the purpose of this work was to evaluate the comprehensive phytochemical profile and biological evaluation of F. Arabica using HPLC method, antimicrobial activity, antioxidants, and cytotoxicity assays. The results showed that the phytochemical components detected in the aerial flowering parts of F. arabica were tannins, steroids, terpenoids, diterpenes, glycosides, saponins, flavonoids, phenols, alkaloids, fixed oils, carbohydrates, proteins, and amino acids. HPLC highlighted the presence of fifteen phenolic compounds present in F. arabica aqueous extract, and the highest of them were chlorogenic acid, gallic acid, and catechin. The antibacterial study of F. arabica extract showed significant inhibitory actions against Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii with zone diameters of 20.0, 22.0, 23.0, 21.0, 17.0, and 18.0 mm, respectively. The extract of aerial flowering parts of F. arabica showed high value (IC50 = 46.25 µg mL- 1) in its free radical scavenging activity. The F. arabica extract showed low cytotoxicity against the Vero and A549 cell lines with IC50 values of 317.09 µg mL- 1 and 178.08 µg/mL, respectively. Therefore, the F. arabica extract is used as antibacterial and anticancer agents due to their high content of phytochemicals and antioxidants and can be applied in innovative pharmaceutical and medical fields.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"114"},"PeriodicalIF":3.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12316655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction Note: Novel adjuvant nano-vaccine induced immune response against Acinetobacter baumannii. 撤稿注：新型佐剂纳米疫苗诱导免疫应答鲍曼不动杆菌。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-08-01 DOI: 10.1186/s13568-025-01923-4

Tohid Piri-Gharaghie, Abbas Doosti, Seyed Abbas Mirzaei

引用次数: 0

Characterization and genomics of phage Henu2_3 against K1 Klebsiella pneumoniae and its efficacy in animal models. 抗K1肺炎克雷伯菌噬菌体Henu2_3的鉴定、基因组学及动物模型疗效研究

IF 3.7 3区生物学

AMB Express Pub Date : 2025-07-30 DOI: 10.1186/s13568-025-01919-0

Qiming Li, Jiaqi Li, Yanyang Zhao, Shuai Guo, Mengzhe Liu, Xiaoyu Shi, Li Wang, Zhigang Liu, Tieshan Teng

{"title":"Characterization and genomics of phage Henu2_3 against K1 Klebsiella pneumoniae and its efficacy in animal models.","authors":"Qiming Li, Jiaqi Li, Yanyang Zhao, Shuai Guo, Mengzhe Liu, Xiaoyu Shi, Li Wang, Zhigang Liu, Tieshan Teng","doi":"10.1186/s13568-025-01919-0","DOIUrl":"10.1186/s13568-025-01919-0","url":null,"abstract":"The emergence and spread of multidrug-resistant and super-resistant bacterial strains have positioned phage therapy as a highly promising approach for future infection treatments. Klebsiella pneumoniae, a leading cause of clinical infections and hospital-acquired diseases, is responsible for over 95% of infections within the Klebsiella genus. Phages targeting K. pneumoniae exhibit remarkable diversity, with numerous isolates identified against various serotypes of this pathogen. In this study, we described the isolation and characterization of a novel bacteriophage from hospital sewage, Henu2_3, which targeted clinical isolates of K1-type K. pneumoniae. Transmission electron microscopy revealed that phage Henu2_3 possessed an icosahedral head and podovirus morphotype. The phage genome comprises 42,878 base pairs with a G + C content of 53.97%, encoding 54 putative open reading frames. One-step growth curve analysis demonstrated that phage Henu2_3 has a latency period of 10 min and an average burst size of 215 phage particles per infected cell. Additionally, Henu2_3 exhibited remarkable stability, tolerating temperatures up to 60℃ and maintaining maximum viability across a broad pH range of 4 to 12. In vivo infection models demonstrated that phage Henu2_3 markedly enhanced animal survival and decreased bacterial burden in target organs. These properties highlight its potential as a therapeutic agent against K. pneumoniae infections.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"112"},"PeriodicalIF":3.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12311074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SETDB1 knockdown boosts recombinant protein in CHO cells via epigenetic transcriptional silencing. SETDB1敲低通过表观遗传转录沉默增强CHO细胞中的重组蛋白。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-07-26 DOI: 10.1186/s13568-025-01914-5

Jiang-Tao Lu, Miao Zhang, Yan-Ping Gao, Lu-Lu Yang, Zhao-Ming Cui, Ming-Ming Han, Xi Zhang, Hong-Yan Xu, Xiao-Yin Wang, Le-Le Qiu, Zi-Chun Hua, Tian-Yun Wang, Yan-Long Jia

{"title":"SETDB1 knockdown boosts recombinant protein in CHO cells via epigenetic transcriptional silencing.","authors":"Jiang-Tao Lu, Miao Zhang, Yan-Ping Gao, Lu-Lu Yang, Zhao-Ming Cui, Ming-Ming Han, Xi Zhang, Hong-Yan Xu, Xiao-Yin Wang, Le-Le Qiu, Zi-Chun Hua, Tian-Yun Wang, Yan-Long Jia","doi":"10.1186/s13568-025-01914-5","DOIUrl":"10.1186/s13568-025-01914-5","url":null,"abstract":"Chinese hamster ovary (CHO) cells serve as the predominant mammalian expression system for recombinant protein production. However, clonal heterogeneity and instability in recombinant protein expression remain significant challenges. SET domain bifurcated histone lysine methyltransferase 1 (SETDB1) catalyzes histone 3 lysine 9 trimethylation (H3K9me3), a critical epigenetic modification regulating gene expression. Despite its functional importance, the dynamics of SETDB1 expression and its regulatory impacts in CHO cells remain poorly characterised. Through transcriptomic analysis of high- and low monoclonal antibody (mAb)-producing CHO clones, we identified SETDB1 as a key modulator of mAb expression. Notably, SETDB1 expression exhibited an inverse correlation with recombinant protein levels in transfected CHO cells. SETDB1-knockdown (SETDB1-KD) CHO cells demonstrated significantly enhanced recombinant antibody-specific productivity. The use of small-molecule SETDB1 inhibitors resulted in a comparable enhancement of transgene expression to that observed with SETDB1 silencing. During extended cultivation, recombinant protein production progressively diminished concurrent with declining SETDB1-KD efficacy. This temporal correlation demonstrates that SETDB1-mediated epigenetic regulation is essential for maintaining both recombinant protein titers and chromosomal stability in industrial biomanufacturing processes. Transcriptome dynamics analysis revealed that SETDB1 silencing induces transcriptional pattern remodeling in transfected cells. These findings elucidate SETDB1's regulatory role in CHO cells and provide actionable insights for the optimisation of recombinant protein production through cell engineering strategies.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"110"},"PeriodicalIF":3.7,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12297104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cucurbitacin B alleviates DSS-induced ulcerative colitis by improving gut microbiota disorder in C57BL/6 mice. 葫芦素B通过改善C57BL/6小鼠肠道菌群紊乱来缓解dss诱导的溃疡性结肠炎。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-07-26 DOI: 10.1186/s13568-025-01922-5

Jichen Li, Xiaodi Shen, Xia Wu, Fan Zhao, Wenling Tang, Minglan Wu, Fang Hu, Xingjiang Hu, Fei Wang, Qiao Zhang

{"title":"Cucurbitacin B alleviates DSS-induced ulcerative colitis by improving gut microbiota disorder in C57BL/6 mice.","authors":"Jichen Li, Xiaodi Shen, Xia Wu, Fan Zhao, Wenling Tang, Minglan Wu, Fang Hu, Xingjiang Hu, Fei Wang, Qiao Zhang","doi":"10.1186/s13568-025-01922-5","DOIUrl":"10.1186/s13568-025-01922-5","url":null,"abstract":"Cucurbitacin B (CuB) is a triterpenoid compound derived from various medicinal plants, demonstrating potential anti-inflammatory, antioxidant, and neuroprotective properties, as well as significant anti-tumor effects. However, its efficacy in treating ulcerative colitis (UC) remains unclear. To investigate the therapeutic potential of CuB, a dextran sulfate sodium (DSS)-induced UC model in mice was employed, along with gut microbiota analysis. The results revealed that CuB significantly alleviated clinical symptoms, improved colonic tissue damage, and suppressed pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 in colon tissues. Additionally, CuB was associated with changes in specific microbial populations, including the upregulation of Muribaculaceae, Rikenellaceae_RC9_gut_group, Muribaculum, and Bifidobacterium, and the downregulation of Desulfovibrionaceae, Helicobacter, Escherichia-Shigella, Streptococcus, Candidatus_Saccharimonas, and Clostridium, which may contribute to the recovery of colon injury. This study provides preliminary evidence supporting CuB's therapeutic potential in DSS-induced colitis by enhancing gut microbiota diversity. CuB shows promise as potential treatment for UC and other conditions related to disruptions in intestinal flora homeostasis.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"111"},"PeriodicalIF":3.7,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12297160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0