IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-26 DOI: 10.1186/s13568-025-01914-5

Jiang-Tao Lu, Miao Zhang, Yan-Ping Gao, Lu-Lu Yang, Zhao-Ming Cui, Ming-Ming Han, Xi Zhang, Hong-Yan Xu, Xiao-Yin Wang, Le-Le Qiu, Zi-Chun Hua, Tian-Yun Wang, Yan-Long Jia

{"title":"SETDB1 knockdown boosts recombinant protein in CHO cells via epigenetic transcriptional silencing.","authors":"Jiang-Tao Lu, Miao Zhang, Yan-Ping Gao, Lu-Lu Yang, Zhao-Ming Cui, Ming-Ming Han, Xi Zhang, Hong-Yan Xu, Xiao-Yin Wang, Le-Le Qiu, Zi-Chun Hua, Tian-Yun Wang, Yan-Long Jia","doi":"10.1186/s13568-025-01914-5","DOIUrl":"https://doi.org/10.1186/s13568-025-01914-5","url":null,"abstract":"Chinese hamster ovary (CHO) cells serve as the predominant mammalian expression system for recombinant protein production. However, clonal heterogeneity and instability in recombinant protein expression remain significant challenges. SET domain bifurcated histone lysine methyltransferase 1 (SETDB1) catalyzes histone 3 lysine 9 trimethylation (H3K9me3), a critical epigenetic modification regulating gene expression. Despite its functional importance, the dynamics of SETDB1 expression and its regulatory impacts in CHO cells remain poorly characterised. Through transcriptomic analysis of high- and low monoclonal antibody (mAb)-producing CHO clones, we identified SETDB1 as a key modulator of mAb expression. Notably, SETDB1 expression exhibited an inverse correlation with recombinant protein levels in transfected CHO cells. SETDB1-knockdown (SETDB1-KD) CHO cells demonstrated significantly enhanced recombinant antibody-specific productivity. The use of small-molecule SETDB1 inhibitors resulted in a comparable enhancement of transgene expression to that observed with SETDB1 silencing. During extended cultivation, recombinant protein production progressively diminished concurrent with declining SETDB1-KD efficacy. This temporal correlation demonstrates that SETDB1-mediated epigenetic regulation is essential for maintaining both recombinant protein titers and chromosomal stability in industrial biomanufacturing processes. Transcriptome dynamics analysis revealed that SETDB1 silencing induces transcriptional pattern remodeling in transfected cells. These findings elucidate SETDB1's regulatory role in CHO cells and provide actionable insights for the optimisation of recombinant protein production through cell engineering strategies.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"110"},"PeriodicalIF":3.5,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Zalaria obscura Y1223 hydrolases: implications for lignocellulose conversion. 黑扎霉Y1223水解酶的表征：木质纤维素转化的意义。

IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-23 DOI: 10.1186/s13568-025-01907-4

Jeremy J Boonzaier, Rodney S Hart, Justin W Hoff, Heinrich W du Plessis, Riaan den Haan

{"title":"Characterization of Zalaria obscura Y1223 hydrolases: implications for lignocellulose conversion.","authors":"Jeremy J Boonzaier, Rodney S Hart, Justin W Hoff, Heinrich W du Plessis, Riaan den Haan","doi":"10.1186/s13568-025-01907-4","DOIUrl":"10.1186/s13568-025-01907-4","url":null,"abstract":"Overconsumption of fossil fuel reserves and its adverse effects has sparked interest in the production of second-generation biofuels due to the abundance of lignocellulosic waste and potential energy crops. However, processing costs associated with depolymerization of the cellulose crystalline structure have stalled advancement in cellulosic ethanol production. Current investigations range from identification of novel enzymes for lignocellulose hydrolysis to consolidation of enzyme production into a singular alcohol producing microorganism to potentially reduce cost for commercial processing. In this study, a total of 828 non-Saccharomyces and black yeasts were screened for cellulolytic and xylanolytic enzyme activities, whereby 60 isolates were identified that exhibited activity for at least one of the enzymes tested. In doing so, a novel Zalaria obscura strain (Z. obscura Y1223) was identified and assessed for enzyme activity in multiple growth media. Semi-quantitative assays showed that Z. obscura Y1223 produced cellulases optimally in media containing yeast extract, peptone and oat bran, with a pH range between pH 5 and 6 and at 30 °C. Maximum xylanase activity (20.5 U/L/OD600) was attained using synthetic complete media supplemented with xylo-oligosaccharides and maximum cellulase activity (7.51 U/L/OD600 endoglucanase, 1.302 U/L/OD600 β-glucosidase) was attained when grown in media containing yeast extract, peptone and oat bran. To our knowledge, this is the first study to quantify the cellulolytic and xylanolytic enzyme activities of a Zalaria spp., which provides key insight into the availability of unexplored cellulolytic enzymes that could inform the design of organisms engineered for consolidated bioprocessing.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"109"},"PeriodicalIF":3.5,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12287495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Milk yield, rumen fermentation, and microbiota of Shami goats fed diets supplemented with spirulina and yeast. 添加螺旋藻和酵母对沙米山羊产奶量、瘤胃发酵和微生物群的影响。

IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-21 DOI: 10.1186/s13568-025-01916-3

Alaa Emara Rabee, Moustafa Mohamed M Ghandour, Ahmed M Sallam, Osama Raef, Eman A Elwakeel, Ebrahim A Sabra, Adel M Abdel-Wahed, Salah Abo Bakr, Hanan Saad ElSamahy, Amal Amin Hamed, Mebarek Lamara

{"title":"Milk yield, rumen fermentation, and microbiota of Shami goats fed diets supplemented with spirulina and yeast.","authors":"Alaa Emara Rabee, Moustafa Mohamed M Ghandour, Ahmed M Sallam, Osama Raef, Eman A Elwakeel, Ebrahim A Sabra, Adel M Abdel-Wahed, Salah Abo Bakr, Hanan Saad ElSamahy, Amal Amin Hamed, Mebarek Lamara","doi":"10.1186/s13568-025-01916-3","DOIUrl":"10.1186/s13568-025-01916-3","url":null,"abstract":"Microalgae and live yeast have gained interest in improving animal performance. This study evaluated the effect of supplementation with Spirulina platensis, Saccharomyces cerevisiae, or their combination on the in vitro and in vivo rumen fermentation, rumen microbiota, and milk yield and composition of lactating Shami goats. The in vitro experiment included four diets: non-supplemented basal diet consisted of Alfalfa hay and a concentrate feed mixture (C); basal diet supplemented with 1% Saccharomyces (Y) based on dry matter; basal diet supplemented with 1% Spirulina (A); and basal diet supplemented with 1% of a mixture of Saccharomyces and Spirulina (AY). In the in vivo experiment, twenty-one lactating goats were divided into three groups (n = 7) to receive one of three diets: C, A, and AY. Group AY had higher in vitro gas production, dry matter digestion (DMD), and volatile fatty acids (VFA) (p < 0.05). Milk yield and feed efficiency were higher in groups A and AY compared to group C. Group AY goats exhibited higher rumen total VFA, acetic, and propionic, while group A showed higher butyric acid. Lower predicted methane was observed in group AY. Groups A and AY showed distinctive microbial communities. The bacterial community was dominated by phylum Bacteroidota, and genera Prevotella and Rikenellaceae RC9 gut group, which were higher in the AY group. The archaeal community was dominated by the genus Methanobrevibacter, which had a lower prevalence in group AY. The combination of live yeast and Spirulina improved rumen fermentation and the milk yield; therefore, it could be used as a feed additive for lactating goats.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"108"},"PeriodicalIF":3.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12279650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin-conjugated magnetic nanoparticles inhibit Staphylococcus aureus growth and biofilm formation via downregulation of Coa and Hla genes. 槲皮素偶联磁性纳米颗粒通过下调辅酶a和Hla基因抑制金黄色葡萄球菌生长和生物膜形成。

IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-15 DOI: 10.1186/s13568-025-01915-4

Alireza Habibi, Masoumeh Pakpour Roudsari, Seyyed Mahdi ZiaZiabari

{"title":"Quercetin-conjugated magnetic nanoparticles inhibit Staphylococcus aureus growth and biofilm formation via downregulation of Coa and Hla genes.","authors":"Alireza Habibi, Masoumeh Pakpour Roudsari, Seyyed Mahdi ZiaZiabari","doi":"10.1186/s13568-025-01915-4","DOIUrl":"10.1186/s13568-025-01915-4","url":null,"abstract":"The research investigated the effects of that magnetic nanoparticles coated with the plant-based flavonoid quercetin have on the growth of Staphylococcus aureus. The synthesis of magnetic nanoparticles proceeded through co-precipitation and further involved quercetin coating using a dextran stabilizer. A combined product of these materials received the chemical name Fe3O4@Dex-QT. The study included multiple analytical tests such as FTIR (Fourier Transform Infrared Spectroscopy), FE-SEM (Field Emission Scanning Electron Microscopy), EDS (Energy-Dispersive X-Ray Spectroscopy) and XRD (X-ray diffraction) together with cellular and molecular assays. Biofilm detection experiments showed that 95% of studied microorganisms had moderate-to-strong biofilm formation abilities. The examined strains that formed biofilms exhibited complete biofilm inhibition at a minimum concentration of 512 μg/mL. Post-treatment with Fe3O4@Dex-QT nanoparticles, the Coa and Hla genes were expressed at 30 and 20% levels respectively, as determined through RT-PCR analysis. The research uncovered that Fe3O4@Dex-QT nanoparticles demonstrate successful antibacterial properties against S. aureus strains, making them viable for substituting conventional antibiotics.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"107"},"PeriodicalIF":3.5,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12263539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mendelian randomization analysis of gut microbiota-immune cell interactions in malignant neoplasm of nasopharynx. 鼻咽部恶性肿瘤中肠道微生物-免疫细胞相互作用的孟德尔随机分析。

IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-15 DOI: 10.1186/s13568-025-01909-2

Qicong Chen, Gang Wang, Jingcheng Shu, Xiaosu Zou, Weiwei Miao, Wenqian Nong, Min Li, Guiping Lan, Wenlin Huang, Xueying Huang, Honglin Luo, Shenhong Qu

{"title":"Mendelian randomization analysis of gut microbiota-immune cell interactions in malignant neoplasm of nasopharynx.","authors":"Qicong Chen, Gang Wang, Jingcheng Shu, Xiaosu Zou, Weiwei Miao, Wenqian Nong, Min Li, Guiping Lan, Wenlin Huang, Xueying Huang, Honglin Luo, Shenhong Qu","doi":"10.1186/s13568-025-01909-2","DOIUrl":"10.1186/s13568-025-01909-2","url":null,"abstract":"Observational studies have suggested associations among the gut microbiome, immune cells, and the risk of malignant neoplasms of nasopharynx. However, the causality of these relationships remains unclear. Thus, we conducted multiple Mendelian Randomization analyses to estimate the causal association of gut microbiota with the risk of malignant neoplasms of nasopharynx and to evaluate the mediating effect of immune cells on this causal pathway. Genetic variants extracted from genome-wide association studies of human gut microbiota compositions (n = 211), immune cell traits (n = 731) and malignant neoplasms of nasopharynx served as instrumental variables for calculating causal associations and mediating effects. Four gut microbiota compositions and eight immune cell traits exhibited detrimental causal effects, while three gut microbiota compositions and fifteen immune cell traits demonstrated protective effects. Interestingly, the causal association of genus Candidatus Soleaferrea id.11350 was no longer significant after adjusting for two established immune cell traits (HLA DR + + monocyte %leukocyte and HLA DR + + monocyte % monocyte). Moreover, HLA DR + + monocyte %leukocyte exhibited a mediating effect (OR 0.75, 95% CI 0.59-0.96) on the causal pathway of genus Candidatus Soleaferrea id.11350-malignant neoplasms of nasopharynx, with a mediating proportion of 21.59%. To our knowledge, this study is the first to identify potential therapeutic targets and elucidate mechanistic insights for malignant neoplasms of nasopharynx interventions involving gut microbiota and immune cell traits; however, these findings warrant further validation through adequately powered randomized clinical trials (RCTs).","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"106"},"PeriodicalIF":3.5,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12263519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expediting multiple biological properties of main bioactive compounds of Mentha pulegium L. 促进薄荷主要生物活性化合物的多种生物学特性。

IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-14 DOI: 10.1186/s13568-025-01911-8

Nasreddine El Omari, Oumayma Aguerd, Abdelaali Balahbib, Naoual El Menyiy, Mohammed Amanullah, Jactty Chew, Long Chiau Ming, Abdelhakim Bouyahya

{"title":"Expediting multiple biological properties of main bioactive compounds of Mentha pulegium L.","authors":"Nasreddine El Omari, Oumayma Aguerd, Abdelaali Balahbib, Naoual El Menyiy, Mohammed Amanullah, Jactty Chew, Long Chiau Ming, Abdelhakim Bouyahya","doi":"10.1186/s13568-025-01911-8","DOIUrl":"10.1186/s13568-025-01911-8","url":null,"abstract":"This study aimed to evaluate the antidiabetic, dermatoprotective, and antibacterial properties of Mentha pulegium L. essential oil (MPEO), harvested in Ouezzane, northwest Morocco. Gas chromatography-mass spectrometry (GC-MS) analysis identified pulegone (41.0%) and menthone (21.2%) as the major constituents. The antibacterial activity was assessed using the micro-broth dilution method, while antidiabetic and dermatoprotective effects were evaluated via in vitro inhibition of α-amylase, α-glucosidase, tyrosinase, and elastase. Additionally, molecular docking was used to assess interactions between key enzymes and major compounds. Both menthone and pulegone exhibited strong antibacterial effects against Staphylococcus aureus and Listeria monocytogenes, with MIC and MBC values ranging from 0.125 to 0.25 µg/mL. Remarkably, menthone and pulegone demonstrated MIC = MBC = 0.125 µg/mL against S. aureus and L. monocytogenes, respectively. In antidiabetic assays, all tested compounds outperformed acarbose. Menthone showed the best inhibition with IC₅₀ values of 149.32 ± 4.16 and 108.39 ± 4.08 μg/mL for α-amylase and α-glucosidase, respectively. MPEO also displayed potent dermatoprotective activity, with IC₅₀ values of 56.23 ± 2.24 μg/mL (tyrosinase) and 90.36 ± 2.26 μg/mL (elastase), compared to quercetin. In silico analysis confirmed strong binding affinities of pulegone and menthone to target enzymes involved in glucose regulation. These findings highlight MPEO as a promising natural source of bioactive compounds with antibacterial, antidiabetic, and dermatoprotective properties, supporting its potential use in pharmaceutical and therapeutic applications.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"105"},"PeriodicalIF":3.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12259508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design a multi-epitope vaccine candidate against Acinetobacter baumannii using advanced computational methods. 利用先进的计算方法设计一种针对鲍曼不动杆菌的多表位候选疫苗。

IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-12 DOI: 10.1186/s13568-025-01913-6

Hana Heidarinia, Elahe Tajbakhsh, Yadollah Bahrami, Mosayeb Rostamian

{"title":"Design a multi-epitope vaccine candidate against Acinetobacter baumannii using advanced computational methods.","authors":"Hana Heidarinia, Elahe Tajbakhsh, Yadollah Bahrami, Mosayeb Rostamian","doi":"10.1186/s13568-025-01913-6","DOIUrl":"10.1186/s13568-025-01913-6","url":null,"abstract":"Acinetobacter baumannii is a significant cause of hospital-acquired infections and is often resistant to multiple antibiotics. In this study, we designed a multi-epitope vaccine candidate using the outer membrane protein K (OmpK) of A. baumannii. T-cell and B-cell epitopes were predicted, and the best epitopes were selected for multi-epitope design. For selecting the best epitopes, many in silico studies, as well as molecular docking of epitope-HLAs, were performed. The multi-epitope was designed using β-defensin as an adjuvant, PADRE sequence as an immunogenicity enhancer, and appropriate linkers. The tertiary structure of the multi-epitope was obtained using modeling and several molecular dynamics (MD)-based refinements. The sequence and/or 3D model of the multi-epitope was investigated for physicochemical, structural, in silico cloning, conformational B epitope prediction, immune response simulation, molecular docking for assay binding to toll-like receptors (TLRs), and deformability studies. The results showed that the multi-epitope construct is favorable in the case of immunogenicity, physicochemical properties, structure, binding to TLRs, solubility, stability, toxicity, allergenicity, and cross-reactivity. This multi-epitope vaccine candidate has the potential to elicit multiple immune responses against A. baumannii. However, in vitro and in vivo experimental tests are needed to validate its efficacy as a potential vaccine candidate.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"103"},"PeriodicalIF":3.5,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking Reishi's secrets: nutritional and medicinal traits of Ganoderma lucidum isolated from tree bark in Egypt. 解开灵芝的秘密：从埃及树皮中分离的灵芝的营养和药用特性。

IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-12 DOI: 10.1186/s13568-025-01905-6

Ramy M Shady, Mona H El-Gayar, Eman El-Gebaly, Hatem S Abbass, Mahmoud A Yassein

{"title":"Unlocking Reishi's secrets: nutritional and medicinal traits of Ganoderma lucidum isolated from tree bark in Egypt.","authors":"Ramy M Shady, Mona H El-Gayar, Eman El-Gebaly, Hatem S Abbass, Mahmoud A Yassein","doi":"10.1186/s13568-025-01905-6","DOIUrl":"10.1186/s13568-025-01905-6","url":null,"abstract":"Ganoderma lucidum is an edible mushroom with high consumption rates in many cultures especially East Asian. This study assessed the in-vitro antimicrobial, antitumor, antiviral as well as antioxidant capacity of Ganoderma lucidum water extract (GLWE). Different microorganisms (standard strain bacteria and clinical isolates) were affected by mushroom aqueous extract, with inhibition zone diameters ranging between 16.1 and 38.8 mm. The MTT assay was used to evaluate antitumor efficacy against three distinct tumor cell lines: HepG-2 (liver tumor cell line), MCF-7 (breast tumor cell line), in addition to Caco2 (colorectal carcinoma cell line). GLWE was found to be safe for Vero cells (CC50 5237 ± 1.6 µg/mL) and had detectable anticancer efficacy against all tested cell lines with IC50 ranges from 99.06 to 198.1 µg/mL. The Petricevich and Mendonça technique was used to test the antiviral activity against herpes simplex virus-1 together with Human adeno virus-7. The findings demonstrated modest antiviral activity, with EC₅₀ values of 493.8 ± 0.98 and 607.1 ± 1.9 µg/mL and corresponding selectivity indices of 10.6 and 8.62, respectively. On studying the antioxidant capacity of GLWE by using DPPH and ABTS, a potential activity with IC50 values of 23.69 ± 0.85 and 14.79 ± 0.92 µg /mL, respectively, were obtained. Liquid chromatography-mass spectrometry in addition to high-performance liquid chromatography analysis indicated a diverse range of compounds including Ganoderic acids, Lucidenic acids, Ganolucidic acids, and Ganoderenic acids which are characteristic triterpenoids to the genus Ganoderma, as well as some polyphenols and flavonoids. In addition, some water/fat soluble vitamins were also detected, as well as proteins and carbohydrates contributing to the high nutritional value of the studied mushroom.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"104"},"PeriodicalIF":3.5,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative evaluation of live attenuated and killed tachyzoites as vaccine candidates for toxoplasmosis. 减毒活速虫与灭活速虫作为弓形虫病候选疫苗的比较评价。

IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-10 DOI: 10.1186/s13568-025-01889-3

Eman E El Shanawany, Eman H Abdel-Rahman, Waleed A Nemr, Soad E Hassan, Noha M F Hassan, Hassan M Desouky, Rabab Zalat, Amany Ebrahim Nofal, Raafat M Shaapan, Salwa Sami Younis

引用次数: 0

Monitoring complete ammonia oxidizers bacteria: relevant players for nitrogen removal from wastewater. 监测完全氨氧化细菌：废水中氮去除的相关参与者。

IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-04 DOI: 10.1186/s13568-025-01878-6

Javier Duque, Leire Besga-Oyanarte, Miguel De Celis, Susana Serrano, José Luis Alonso, Antonio Santos, Lucía Arregui

{"title":"Monitoring complete ammonia oxidizers bacteria: relevant players for nitrogen removal from wastewater.","authors":"Javier Duque, Leire Besga-Oyanarte, Miguel De Celis, Susana Serrano, José Luis Alonso, Antonio Santos, Lucía Arregui","doi":"10.1186/s13568-025-01878-6","DOIUrl":"10.1186/s13568-025-01878-6","url":null,"abstract":"COMAMMOX (Complete Ammonia Oxidizers) bacteria seem to play an important role in nitrification, being able to directly transform ammonia into nitrates. Due to the relevance of this process in WWTPs, different approaches were used in this work to monitor COMAMMOX bacteria, as well as other microorganisms involved in the biological removal of nitrogen occurring in the bioreactor of a full-scale WWTP. This facility operates with low dissolved oxygen concentrations and prolonged sludge retention times. Fluorescence in situ hybridization confirmed the presence of COMAMMOX bacteria showing their distribution within the activated sludge flocs. Primers targeting the amoA gene, which encodes the A subunit of the ammonia monooxygenase enzyme, were employed as genetic markers in PCR techniques to simultaneously detect and amplify both COMAMMOX clades A and B, as well as specifically clade A, clade B, or various COMAMMOX Nitrospira species (Ca. N. nitrosa, Ca. N. inopinata, and Ca. N. nitrificans). Using digital PCR, a set of these primers enabled the estimation of the proportion of amoA COMAMMOX genes versus other nitrifiers (AOB, AOA and NOB). The data obtained not only confirmed the presence of COMAMMOX bacteria, but also their abundance, comparable to that of NOB and AOB. The 16S rRNA gene-based meta-taxonomy analysis gave complementary data about the microbial diversity and community composition within the samples. The results also highlight the biases of each technology used as monitoring tools, which must be accurate for the effective management of wastewater treatment plants (WWTPs) and for exploiting the potential of COMAMMOX bacteria.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"101"},"PeriodicalIF":3.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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