Mastoparan-S from Sphodromantis viridis exhibits antimicrobial activity by disrupting bacterial membranes.

IF 3.7 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jonggwan Park, Seulbi Lee, Da Dam Kang, Jun Hee Oh, Hyeongsun Kim, Yoonkyung Park
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引用次数: 0

Abstract

The increasing prevalence of antimicrobial resistance underscores the urgent need for alternative treatments. Antimicrobial peptides (AMPs) are considered viable substitutes for conventional antibiotics due to their broad-spectrum activity and unique mechanisms of action. AMPs are an integral component of the innate immune system in invertebrates. Mastoparan-S, a 14-amino acid AMP originally identified in the praying mantis Sphodromantis viridis reportedly exhibits antimicrobial activity against gram-positive and gram-negative bacteria, as well as fungi. In this study, the antibacterial activity and mechanism of action of chemically-synthesized mastoparan-S are evaluated against Staphylococcus aureus and Pseudomonas aeruginosa. Mastoparan-S exhibits potent antibacterial activity against S. aureus and P. aeruginosa, without inducing cytotoxicity or hemolytic activity in RAW264.7 or HaCaT cells. Mastoparan-S effectively inhibits biofilm formation by S. aureus and P. aeruginosa. Mechanistically, mastopran-S kills bacteria by disrupting the bacterial membrane. Collectively, these findings suggest that mastoparan-S is a potential therapeutic agent for treating S. aureus and P. aeruginosa infections.

来自病毒性眼炎的Mastoparan-S通过破坏细菌膜表现出抗菌活性。
抗菌素耐药性的日益流行突出表明迫切需要替代治疗。抗菌肽因其广谱活性和独特的作用机制被认为是传统抗生素的可行替代品。amp是无脊椎动物先天免疫系统的一个组成部分。Mastoparan-S是一种由14个氨基酸组成的AMP,最初在螳螂(phhodromantis viridis)中发现,据报道,它对革兰氏阳性和革兰氏阴性细菌以及真菌具有抗菌活性。本研究对化学合成的mastoparan-S对金黄色葡萄球菌和铜绿假单胞菌的抑菌活性和作用机制进行了评价。Mastoparan-S对金黄色葡萄球菌和铜绿假单胞菌具有有效的抗菌活性,而不会对RAW264.7或HaCaT细胞产生细胞毒性或溶血活性。Mastoparan-S有效抑制金黄色葡萄球菌和铜绿假单胞菌形成生物膜。从机械上讲,mastopran-S通过破坏细菌膜来杀死细菌。总之,这些发现表明,mastoparan-S是治疗金黄色葡萄球菌和铜绿假单胞菌感染的潜在治疗药物。
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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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