{"title":"采用片上多路等温扩增的自主微流控流感A/B亚型系统，用于现场可部署监测。","authors":"Si-Ming Lu, Jia-Long Wang, Yang Li, Ling Zhang, Hang-Ping Yao, Li-Guo Liang","doi":"10.1186/s13568-025-01933-2","DOIUrl":null,"url":null,"abstract":"<p><p>Influenza viruses present significant challenges to global health. A rapid recombinase polymerase amplification (RPA)-based detection system for multiple influenza strains (A, H1N1/H2N2/H3N2/H5N1/H7N9 and B) has been developed to address the limitations of 2-h Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) tests. By using optimized primers targeting key viral proteins (M, NA, HA, PA), the method achieves detection in 10 min with 0.99 log linearity (1-10<sup>6</sup> copies/μL). Clinical validation demonstrated 100% sensitivity and specificity, effectively distinguishing infections from healthy controls. This portable platform shows strong potential for point-of-care (POC) applications in resource-limited settings, offering timely diagnosis and epidemic control through its rapid and accurate detection capabilities.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"123"},"PeriodicalIF":3.7000,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12370608/pdf/","citationCount":"0","resultStr":"{\"title\":\"Autonomous microfluidic influenza A/B subtyping system using on-chip multiplex isothermal amplification for field-deployable surveillance.\",\"authors\":\"Si-Ming Lu, Jia-Long Wang, Yang Li, Ling Zhang, Hang-Ping Yao, Li-Guo Liang\",\"doi\":\"10.1186/s13568-025-01933-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Influenza viruses present significant challenges to global health. A rapid recombinase polymerase amplification (RPA)-based detection system for multiple influenza strains (A, H1N1/H2N2/H3N2/H5N1/H7N9 and B) has been developed to address the limitations of 2-h Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) tests. By using optimized primers targeting key viral proteins (M, NA, HA, PA), the method achieves detection in 10 min with 0.99 log linearity (1-10<sup>6</sup> copies/μL). Clinical validation demonstrated 100% sensitivity and specificity, effectively distinguishing infections from healthy controls. This portable platform shows strong potential for point-of-care (POC) applications in resource-limited settings, offering timely diagnosis and epidemic control through its rapid and accurate detection capabilities.</p>\",\"PeriodicalId\":7537,\"journal\":{\"name\":\"AMB Express\",\"volume\":\"15 1\",\"pages\":\"123\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-08-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12370608/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"AMB Express\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1186/s13568-025-01933-2\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"AMB Express","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1186/s13568-025-01933-2","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Autonomous microfluidic influenza A/B subtyping system using on-chip multiplex isothermal amplification for field-deployable surveillance.
Influenza viruses present significant challenges to global health. A rapid recombinase polymerase amplification (RPA)-based detection system for multiple influenza strains (A, H1N1/H2N2/H3N2/H5N1/H7N9 and B) has been developed to address the limitations of 2-h Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) tests. By using optimized primers targeting key viral proteins (M, NA, HA, PA), the method achieves detection in 10 min with 0.99 log linearity (1-106 copies/μL). Clinical validation demonstrated 100% sensitivity and specificity, effectively distinguishing infections from healthy controls. This portable platform shows strong potential for point-of-care (POC) applications in resource-limited settings, offering timely diagnosis and epidemic control through its rapid and accurate detection capabilities.
期刊介绍：
AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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