AMB Express最新文献

{"title":"Method for automated high performance closed batch cultivation of gas-utilizing methanogens.","authors":"Walter Hofmann, Marco Orthofer, Nicolás Salas Wallach, Aquilla Ruddyard, Markus Ungerank, Christian Paulik, Simon K-M R Rittmann","doi":"10.1186/s13568-025-01872-y","DOIUrl":"https://doi.org/10.1186/s13568-025-01872-y","url":null,"abstract":"<p><p>To advance the utilization of microbial cell factories in gas fermentation processes, their physiological and biotechnological characteristics must be understood. Here, we report on the construction and operation of a novel device, the Gas and Pressure Controller (GPC), which is specifically designed for the automated control of the headspace gas pressure of closed cultivation bottles and facilitates automated gassing, sparging, monitoring and regulation of the headspace volume operated in closed batch cultivation mode in real time.As proof of concept, the physiological and biotechnological characteristics of four autotrophic, hydrogenotrophic methanogenic archaea were examined to quantify novel physiological limits through the elimination of gas limitation during growth and methane formation. We determined unprecedented high maximum specific methane productivity (qCH₄) values for: Methanothermobacter marburgensis of 169.59 ± 12.52 mmol g^- 1 h^- 1, Methanotorris igneus of 420.21 ± 89.46 mmol g^- 1 h^- 1, Methanocaldococcus jannaschii of 364.52 ± 25.50 mmol g^- 1 h^- 1 and Methanocaldococcus villosus of 356.38 ± 20.79 mmol g^- 1 h^- 1. Obtained qCH₄ of M. marburgensis is more than 10-fold higher compared to conventional closed batch cultivation set-ups and as high as the highest reported qCH₄ value of M. marburgensis from fed-batch gas fermentation in stirred tank bioreactors. Furthermore, the GPC demonstrated reliable functionality with Methanococcus maripaludis, operating safely and autonomous during long term cultivation. This novel device enables optimal headspace pressure control, providing flexibility in application for various gas-fermenting biotechnological processes. It facilitates near optimal cultivation conditions in semi-continuous closed batch cultivation mode, the analysis of limiting factors in high-throughput experimental design and allows for automated biomass production of autotrophic, hydrogenotrophic methanogens.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"67"},"PeriodicalIF":3.5,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect evaluation of repairing cement-mortar microbeams by microbial induced carbonate precipitation.","authors":"Peifeng Huang, Xinhua Yang, Yue Dai","doi":"10.1186/s13568-025-01881-x","DOIUrl":"https://doi.org/10.1186/s13568-025-01881-x","url":null,"abstract":"<p><p>The technique of microbially induced calcium carbonate precipitation (MICP) has a bright prospect in the repair of concrete structures with diseases, so the evaluation of the repair effect and its influencing factors are very important issues for civil engineers. In this paper, multi-phase mixed precipitate models are established by using the random particle generation and packing algorithm. Combined with the cohesive zone model, the deformation and failure behavior of notched cement-mortar microbeams before and after repair under three-point bending loading are numerically simulated. The recovery rate is proposed to characterize the repair effect of microbeam. The repair effect and the influences of the proportion of crystalline phases in the precipitate, particle size and notch location on it are evaluated. It is found that the recovery rate of peak load of microbeam decreases from 22.16 to 20.60% as the proportion of calcite increases from 0 to 1 for the combination case of calcite and vaterite in the particles of the precipitate. However, for the combination case of calcite and aragonite, as the proportion of calcite increases from 0 to 1, the recovery rate of peak load decreases from 35.01 to 20.77%. For only calcite grains as the particles of the precipitate, the recovery rate of peak load increases from 12.73 to 36.85% when the particle size increases from 2  to 3.4 μm. When the distance between the notch center and the microbeam midspan increases from 0 to 40 μm, the recovery rate of peak load increases from 20.44 to 77.26%. The effects of the proportion of crystalline phases, particle size and notch location on the repairing effect of microbeams can be explained from the population of matrix-particle interface and stress concentration degree in precipitate. Considering that the precipitate compositions can be regulated by the control of environmental and process parameters, the research in this paper is of great significance for the engineering application of MICP technique.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"66"},"PeriodicalIF":3.5,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144059102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel thermostable lytic phage vB_EF_Enf3_CCASU-2024-3 against clinical Enterococcus faecium and Enterococcus faecalis.","authors":"Rana M Amr, Amr S Bishr, Bishoy T Saad, Mohammad Y Alshahrani, Khaled M Aboshanab, Nadia A Hassouna","doi":"10.1186/s13568-025-01871-z","DOIUrl":"https://doi.org/10.1186/s13568-025-01871-z","url":null,"abstract":"<p><p>Enterococci, Gram-positive bacteria, have become a major concern in healthcare settings due to their significant virulence and antibiotic resistance. This research focuses on isolating, phenotypic, and genotypic analysis of enterococci-specific lytic phages to be used as potential candidates in combating multidrug-resistant (MDR) Enterococcus clinical isolates. The virulence of Enterococcus isolates was analyzed by testing for gelatinase and biofilm formation. The phage(s) was isolated from a sewage sample, then purified, propagated, and physiochemically analyzed. The phage was examined using transmission electron microscopy, and the whole genome sequence (WGS) was performed. Sixety-five clinical enterococci including, 27 (41.5%), 33 (50.7%) 3 (4.6%), and 2 (3%) E. faecalis, E. faecium, E. avium, and E. durans, respectively were isolated. Linezolid, teicoplanin, chloramphenicol, and vancomycin exhibited the lowest resistance. Twenty-five (38.5%) isolates were both gelatinase- and biofilm-producers. A novel lytic vB_EF_Enf3 phage belonging to Caudoviricetes class, characterized by an icosahedral head with a diameter of 100 ± 5 nm and a tail measuring 70 ± 5 nm in length was isolated. The phage demonstrated good thermal stability, and viability across various pH levels and exhibited a broad- spectrum of activity against E. faecium and E. faecalis. The vB_EF_Enf3 phage (36,202 bp length) harbored 36 open reading frames (ORFs) with a GC content of 34.4% (GenBank accession, PP747318). In conclusion, a novel thermostable lytic bacteriophage vB_EF_Enf3, belonging to class Caudoviricetes, was isolated from sewage showing broad-spectrum potent lytic activity against E. faecium and E. faecalis and maintained stability under various extreme conditions, including temperature, and pH fluctuations.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"65"},"PeriodicalIF":3.5,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12033158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of alkaline lipase by Aspergillus terreus AUMC 15762 for laundry application.","authors":"Osama Abdel-Hafeez Mohamed Al-Bedak, Ahmed Mohamed Ahmed Ali Ramadan, Hussein H El-Sheikh, Reda M Shehata","doi":"10.1186/s13568-025-01865-x","DOIUrl":"https://doi.org/10.1186/s13568-025-01865-x","url":null,"abstract":"<p><p>Lipases are extraordinarily critical co-factor-independent enzymes with profound economic consequences. They are utilized extensively in production of fine chemicals, food, textile, pulp and paper, laundry, and biodiesel sectors. In the current study, the lipolytic activity of 141 fungal isolates-representing 21 genera and 38 species-that were isolated from samples of desert soil gathered from the Governorates of Sohag, Qena, and Aswan were examined. Of the 74 isolates showed positive lipase activity, 40 were high lipase producers. In terms of lipase production, Aspergillus terreus AUMC 15762 was the most effective strain. To enhance the synthesis of lipase from Aspergillus terreus AUMC 15762, Plackett-Burman design (PBD) was employed. For the maximal amount of lipase synthesis (103.3 U/mL), ammonium sulphate was required after three days at 25 °C, pH 4.0, and 3.0 g/L. Through the use of Trilite MA 12 anion exchanger and Sephadex G-100 column chromatography, lipase was purified 17.79 times and achieved 64.714 kDa molecular weight on SDS-PAGE. The highest possible specific activity of 3867.85 ± 214.28 U/mg was attained at pH 8.0 and 40 °C. The addition of KCl and ZnSO₄ raised the lipase specific activity by 115.42%. K_m of 19.0 mg/mL and V_max of 1000 μmol/min were determined for the pure lipase. The effects of 20 U/mL pure lipase on corn and olive oily spots were examined in this work at pH 8.0 and 40 °C. The pure lipase completely removed oil contamination from fiber surfaces, as evidenced by the oily spots' separation from the white cotton textiles after 60 min. This work offers a lipase produced from Aspergillus terreus species that showed promise for industrial laundry applications.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"64"},"PeriodicalIF":3.5,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11994561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mendelian randomization study of lipid species reveals causal relationship with syphilis.","authors":"Weifeng Shen, Hui Zhou, Wei Wang, Wei Li","doi":"10.1186/s13568-025-01873-x","DOIUrl":"https://doi.org/10.1186/s13568-025-01873-x","url":null,"abstract":"<p><p>Although some studies have reported a possible association between lipid and the development and progression of syphilis, the overall causal relationship between lipid and syphilis remains unclear. Data abstracted from extensive genome-wide association studies were utilized to pinpoint genetic variations linked to 179 distinct lipid species. Subsequently, these variations served as instrumental variables in Mendelian Randomization (MR) analyses, aimed at evaluating the causal impact of these lipid species on the occurrence of syphilis. A range of methods, including Weighted Mode, Weighted Median, Simple Mode, MR Egger, and Inverse Variance Weighted, were employed to determine the causal influence. For the purpose of sensitivity analysis, techniques such as Inverse Variance Weighted, MR-Egger, the MR Steiger test, the MR-Egger Intercept Test, and MR-PRESSO were applied. Additionally, Multivariate Mendelian Randomization (MVMR) analyses were conducted to directly assess the causal effect of lipid species on the risk of syphilis. Sterol ester (SE) and phosphatidylcholine (PC) could potentially impact syphilis risk. Specifically, SE (27:1/16:0), SE (27:1/18:2), SE (27:1/18:3), SE (27:1/20:3), and SE (27:1/22:6) were linked to an elevated risk of syphilis. PC (18:2_0:0) was linked to an elevated risk of syphilis. In contrast, PC (16:1_18:0) exhibited a protective role against syphilis. No heterogeneity or horizontal pleiotropy was detected. SE (27:1/16:0), SE (27:1/18:2), SE(27:1/18:3), SE (27:1/20:3), and SE (27:1/22:6) were no longer significantly associated with syphilis in the MVMR analysis (P>0.05). In addition, the previously observed effect of PC (18:2_0:0) on syphilis in univariate MR Was no longer significant in MVMR analysis. However, genetically predicted PC (16:1_18:0) was still significantly negatively associated with syphilis, consistent with univariate MR analysis. We observed that hereditary SE and PC levels appear to be associated with syphilis susceptibility. Future research is needed to understand the mechanisms behind this supposed causation and to develop corresponding treatment strategies.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"63"},"PeriodicalIF":3.5,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11981995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of triterpenoids biosynthesis in Athelia termitophila as a source of natural products.","authors":"Fangcheng Hu, Yonggang Fang, Zahid Khan, Lianxi Xing","doi":"10.1186/s13568-025-01840-6","DOIUrl":"10.1186/s13568-025-01840-6","url":null,"abstract":"<p><p>Triterpenoids exhibit considerable potential and are extensively utilized in both food and pharmaceutical industries. However, Athelia termitophila (TMB) naturally harbors only trace amounts of these compounds. Consequently, this study sought to optimize the composition of the culture medium and its cultivation parameters to augment both triterpenoids biosynthesis and TMB biomass. To this end, a series of systematic experiments were carried out. At the outset, a One Factor at a Time (OFAT) approach was employed to identify key culture components and conditions. Based on the OFAT findings, six factors were selected for further investigation using the Plackett-Burman design (PBD) to assess their influence on triterpenoids production and biomass yield. The PBD outcomes pinpointed three critical factors-cultural duration, yeast extract powder, and KH₂PO₄-each of which was subjected to further optimization through the Box-Behnken design (BBD). The BBD analysis determined the optimal culture medium and conditions: 30 g/L corn starch, 13.44 g/L yeast extract powder, 4.74 g/L KH₂PO₄, a liquid-to-volume ratio of 130/250 mL, 6% inoculum volumes, and a cultivation period of 7.8 days. Upon optimization, both triterpenoids yield (1.9-fold increase) and mycelial biomass (1.66-fold increase) were significantly enhanced compared to the unoptimized medium. This study not only provides a robust methodology for enhancing triterpenoids content and mycelial biomass in TMB, but also contributes novel insights into the biosynthesis of triterpenoids.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"61"},"PeriodicalIF":3.5,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Bifidobacterium animalis subsp. lactis BB-12 in mice with acute pancreatitis.","authors":"Bingbing Du, Ren Yan, Xiaoxiang Hu, Jing Lou, Yixin Zhu, Yini Shao, Huiyong Jiang, Yingying Hao, Longxian Lv","doi":"10.1186/s13568-025-01867-9","DOIUrl":"10.1186/s13568-025-01867-9","url":null,"abstract":"<p><p>Acute pancreatitis (AP) is a prevalent acute gastrointestinal disease, which may be prevented and alleviated by probiotics. Bifidobacterium animalis subsp. lactis BB-12 (BB-12) is a widely studied probiotic strain; however, its specific effects in this context remain unexplored. In this study, we aimed to investigate the prophylactic and therapeutic effects of BB-12 in AP. Our findings revealed that BB-12 administration via gavage significantly reduced pathological pancreatic damage and serum amylase activity. Microbiome analysis showed that BB-12 treatment significantly increased the relative abundance of Ligilactobacillus and decreased that of Bilophila in the gut microbiota of mice with AP. Transcriptome analysis revealed that BB-12 mitigated the AP-induced dysregulation of several pathways, specifically attenuating the upregulation of the pancreatic secretion and ascorbate and aldarate metabolism pathways while reversing the downregulation of the ribosome, oxidative phosphorylation, and thermogenesis pathways. Spearman's correlation analysis revealed a positive correlation between the abundances of Bilophila and ASF356 and serum amylase activity. Furthermore, the abundances of Bilophila and ASF356 were significantly correlated with BB-12-regulated pancreatic genes and were predominantly enriched in the ribosome pathway. In conclusion, BB-12 pretreatment alleviated AP, likely by regulating the abundance of intestinal Lactobacillus, Bilophila, and ASF356, as well as the pancreatic secretion, ascorbate and aldarate metabolism, oxidative phosphorylation, ribosome, and thermogenesis pathways.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"62"},"PeriodicalIF":3.5,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotransformation of quercetin by Bacillus subtilis and anticancer activity evaluation: in vitro and in Silico.","authors":"Salsabeel N El Gendy, Amira K Elmotayam, Reham Samir, Marwa I Ezzat, Mahmoud T Abo-Elfadl, Aly M El Sayed","doi":"10.1186/s13568-025-01860-2","DOIUrl":"10.1186/s13568-025-01860-2","url":null,"abstract":"<p><p>Biotransformation of quercetin by Bacillus subtilis ATCC 23,857 yielded a major metabolite; apigenin-7-O-pentoside (A7P) and 9 other phenolic metabolites. They were elucidated by LC-ESI-TOF-MS/MS analysis. Furthermore, the cytotoxic potential of the biotransformed products was compared to their parent substrate (S) using MTT assay against 2 cancer cell lines; A549 and Caco-2. After 72 h. incubation, the BPs showed concentration-dependent cytotoxic activity against Caco-2 and A549 with IC₅₀ equal to 0.32 and 0.78 mg/ml, respectively. Both BPs and S were safe on the normal human skin fibroblast (hFB) cell line. Molecular docking confirmed the high binding affinity of A7P towards phosphatidyl inositide 3-kinase delta (PI3Kδ) and Cathepsin B protein (CatB) expressed in lung and colorectal cancer cells compared to quercetin suggesting higher anticancer activity. The present findings suggest that the application of biotransformation techniques using bacteria as B. subtilis can enhance the biological activity of flavonoids by generating bioactive metabolites.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"58"},"PeriodicalIF":3.5,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11965081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the microorganisms biodiversity associated with sponge species in the red sea through 18S ribosomal RNA gene sequencing.","authors":"Samah S Abuzahrah","doi":"10.1186/s13568-025-01863-z","DOIUrl":"10.1186/s13568-025-01863-z","url":null,"abstract":"<p><p>Around the world, sponges play a significant role in marine ecosystems, and a wide variety of sponge species can be found in the coast of Red Sea of Saudi Arabia. The unique environmental conditions of the Red Sea, including warm, oligotrophic water and high salinity, have encouraged the growth of abundant sponge fauna. Our study aims to investigate the biodiversity, taxonomic composition, and phylogenetic relationships of eukaryotic organisms linked with sponges in the Red Sea off the coast of Saudi Arabia and infer the possible ecological roles and functional contributions of the identified eukaryotic taxa to sponge health and ecosystem functioning. The study investigated the microbial diversity, focusing on the genera Hyalosynedra sp., Navicula sp., Papiliocellulus sp., Psammodictyon sp., Pynococcus sp., Ostreococcus sp., Micromonas sp., and other unclassified species. Our metagenomic analysis and phylogenetic evaluation revealed a deep and diverse microbial community, with each genus performing significant ecological roles, including nutrient cycling, primary production, and contributing to marine food networks. Moreover, these genera display promising biotechnological prospects, including uses in bioremediation, biofuel production, and the synthesis of high-value biomolecules. Comparative analysis with other marine regions has focused on both the similarities and unique aspects of the Red Sea microbial community, which are influenced by its distinct environmental conditions. The gained findings contribute to a deeper understanding of the ecological dynamics in the Red Sea and open new avenues for biotechnological exploration in marine ecosystems.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"60"},"PeriodicalIF":3.5,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11965075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota, blood metabolites, & pan-cancer: a bidirectional Mendelian randomization & mediation analysis.","authors":"Biqing Luan, Yang Yang, Qizhi Yang, Zhiqiang Li, Zhihui Xu, Yaqin Chen, Meiting Wang, Wenlin Chen, Fei Ge","doi":"10.1186/s13568-025-01866-w","DOIUrl":"10.1186/s13568-025-01866-w","url":null,"abstract":"<p><p>We propose using Mendelian randomization analysis on GWAS data and MetaboAnalyst to model gut microbiota, metabolic pathways, blood metabolites, and cancer risk. We examined 473 gut microbiota, 205 pathways, 1400 metabolites, and 8 cancers. Results were validated through bidirectional two-sample Mendelian Randomization (MR), heterogeneity tests, and pathway enrichment, leading to a mediation pathway model. We identified 129 gut microbiota, 57 pathways, and 463 metabolites linked to cancer, and 34 significant plasma pathways. 15 microbiota, 8 pathways, and 58 metabolites implicated in multiple cancers. Eight plasma metabolic pathways are involved in the development of multiple types of cancer. Through Multivariate Mendelian Randomization (MVMR) and mediation analysis, we found 9 mediation pathways, offering novel targets and research directions for cancer pathogenesis and treatment.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"59"},"PeriodicalIF":3.5,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11965084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}