AMB Express最新文献_第4页

Demographic drivers of gut microbiome diversity. 肠道微生物群多样性的人口驱动因素。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-08-08 DOI: 10.1186/s13568-025-01921-6

Hannaneh Kabir, Matilda Holtz, Justine Choueiri, Mohammad Reza Kaazempur Mofrad

{"title":"Demographic drivers of gut microbiome diversity.","authors":"Hannaneh Kabir, Matilda Holtz, Justine Choueiri, Mohammad Reza Kaazempur Mofrad","doi":"10.1186/s13568-025-01921-6","DOIUrl":"10.1186/s13568-025-01921-6","url":null,"abstract":"The gut microbiome plays a central role in orchestrating metabolic, immune, and neurological functions essential for human health. While extensive research has explored the effects of diseases and pathological conditions on gut microbiome composition, the influence of demographic factors remains underexplored, limiting our understanding of microbiome variations in disease states. This study addresses this gap by investigating the impact of demographic variables, including age, sex, and geography, on gut microbiome diversity in healthy individuals. Using the American Gut Project's extensive dataset and the QIIME2 bioinformatics pipeline, we conducted a comprehensive analysis of microbial profiles across diverse demographic groups. Our results revealed significant age-related shifts in microbial richness and composition, and geographic location strongly influenced phylogenetic diversity. In contrast, sex exhibited limited impact on microbial diversity within healthy BMI ranges. These findings highlight the critical role of demographic factors in shaping gut microbiome diversity, providing a foundational framework to better contextualize disease-related microbiome variations and advance personalized healthcare approaches.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"115"},"PeriodicalIF":3.7,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12334389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into the biological activities of Fagonia Arabica L. and its phytochemical constituents. 阿拉比卡Fagonia L.的生物活性及其植物化学成分研究。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-08-01 DOI: 10.1186/s13568-025-01918-1

Nashaat N Mahmoud, Alsayed E Mekky, Esawy Mahmoud, Mayada M El-Azab, Muhammad I Haggag

{"title":"Insight into the biological activities of Fagonia Arabica L. and its phytochemical constituents.","authors":"Nashaat N Mahmoud, Alsayed E Mekky, Esawy Mahmoud, Mayada M El-Azab, Muhammad I Haggag","doi":"10.1186/s13568-025-01918-1","DOIUrl":"10.1186/s13568-025-01918-1","url":null,"abstract":"In Egypt, the genus Fagonia is found in all the deserts of Egypt and the Sinai Peninsula. It is widely used to treat many diseases as a home remedy without sufficient scientific studies, especially Fagonia arabica L. Therefore, the purpose of this work was to evaluate the comprehensive phytochemical profile and biological evaluation of F. Arabica using HPLC method, antimicrobial activity, antioxidants, and cytotoxicity assays. The results showed that the phytochemical components detected in the aerial flowering parts of F. arabica were tannins, steroids, terpenoids, diterpenes, glycosides, saponins, flavonoids, phenols, alkaloids, fixed oils, carbohydrates, proteins, and amino acids. HPLC highlighted the presence of fifteen phenolic compounds present in F. arabica aqueous extract, and the highest of them were chlorogenic acid, gallic acid, and catechin. The antibacterial study of F. arabica extract showed significant inhibitory actions against Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii with zone diameters of 20.0, 22.0, 23.0, 21.0, 17.0, and 18.0 mm, respectively. The extract of aerial flowering parts of F. arabica showed high value (IC50 = 46.25 µg mL- 1) in its free radical scavenging activity. The F. arabica extract showed low cytotoxicity against the Vero and A549 cell lines with IC50 values of 317.09 µg mL- 1 and 178.08 µg/mL, respectively. Therefore, the F. arabica extract is used as antibacterial and anticancer agents due to their high content of phytochemicals and antioxidants and can be applied in innovative pharmaceutical and medical fields.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"114"},"PeriodicalIF":3.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12316655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction Note: Novel adjuvant nano-vaccine induced immune response against Acinetobacter baumannii. 撤稿注：新型佐剂纳米疫苗诱导免疫应答鲍曼不动杆菌。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-08-01 DOI: 10.1186/s13568-025-01923-4

Tohid Piri-Gharaghie, Abbas Doosti, Seyed Abbas Mirzaei

引用次数: 0

Characterization and genomics of phage Henu2_3 against K1 Klebsiella pneumoniae and its efficacy in animal models. 抗K1肺炎克雷伯菌噬菌体Henu2_3的鉴定、基因组学及动物模型疗效研究

IF 3.7 3区生物学

AMB Express Pub Date : 2025-07-30 DOI: 10.1186/s13568-025-01919-0

Qiming Li, Jiaqi Li, Yanyang Zhao, Shuai Guo, Mengzhe Liu, Xiaoyu Shi, Li Wang, Zhigang Liu, Tieshan Teng

{"title":"Characterization and genomics of phage Henu2_3 against K1 Klebsiella pneumoniae and its efficacy in animal models.","authors":"Qiming Li, Jiaqi Li, Yanyang Zhao, Shuai Guo, Mengzhe Liu, Xiaoyu Shi, Li Wang, Zhigang Liu, Tieshan Teng","doi":"10.1186/s13568-025-01919-0","DOIUrl":"10.1186/s13568-025-01919-0","url":null,"abstract":"The emergence and spread of multidrug-resistant and super-resistant bacterial strains have positioned phage therapy as a highly promising approach for future infection treatments. Klebsiella pneumoniae, a leading cause of clinical infections and hospital-acquired diseases, is responsible for over 95% of infections within the Klebsiella genus. Phages targeting K. pneumoniae exhibit remarkable diversity, with numerous isolates identified against various serotypes of this pathogen. In this study, we described the isolation and characterization of a novel bacteriophage from hospital sewage, Henu2_3, which targeted clinical isolates of K1-type K. pneumoniae. Transmission electron microscopy revealed that phage Henu2_3 possessed an icosahedral head and podovirus morphotype. The phage genome comprises 42,878 base pairs with a G + C content of 53.97%, encoding 54 putative open reading frames. One-step growth curve analysis demonstrated that phage Henu2_3 has a latency period of 10 min and an average burst size of 215 phage particles per infected cell. Additionally, Henu2_3 exhibited remarkable stability, tolerating temperatures up to 60℃ and maintaining maximum viability across a broad pH range of 4 to 12. In vivo infection models demonstrated that phage Henu2_3 markedly enhanced animal survival and decreased bacterial burden in target organs. These properties highlight its potential as a therapeutic agent against K. pneumoniae infections.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"112"},"PeriodicalIF":3.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12311074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SETDB1 knockdown boosts recombinant protein in CHO cells via epigenetic transcriptional silencing. SETDB1敲低通过表观遗传转录沉默增强CHO细胞中的重组蛋白。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-07-26 DOI: 10.1186/s13568-025-01914-5

Jiang-Tao Lu, Miao Zhang, Yan-Ping Gao, Lu-Lu Yang, Zhao-Ming Cui, Ming-Ming Han, Xi Zhang, Hong-Yan Xu, Xiao-Yin Wang, Le-Le Qiu, Zi-Chun Hua, Tian-Yun Wang, Yan-Long Jia

{"title":"SETDB1 knockdown boosts recombinant protein in CHO cells via epigenetic transcriptional silencing.","authors":"Jiang-Tao Lu, Miao Zhang, Yan-Ping Gao, Lu-Lu Yang, Zhao-Ming Cui, Ming-Ming Han, Xi Zhang, Hong-Yan Xu, Xiao-Yin Wang, Le-Le Qiu, Zi-Chun Hua, Tian-Yun Wang, Yan-Long Jia","doi":"10.1186/s13568-025-01914-5","DOIUrl":"10.1186/s13568-025-01914-5","url":null,"abstract":"Chinese hamster ovary (CHO) cells serve as the predominant mammalian expression system for recombinant protein production. However, clonal heterogeneity and instability in recombinant protein expression remain significant challenges. SET domain bifurcated histone lysine methyltransferase 1 (SETDB1) catalyzes histone 3 lysine 9 trimethylation (H3K9me3), a critical epigenetic modification regulating gene expression. Despite its functional importance, the dynamics of SETDB1 expression and its regulatory impacts in CHO cells remain poorly characterised. Through transcriptomic analysis of high- and low monoclonal antibody (mAb)-producing CHO clones, we identified SETDB1 as a key modulator of mAb expression. Notably, SETDB1 expression exhibited an inverse correlation with recombinant protein levels in transfected CHO cells. SETDB1-knockdown (SETDB1-KD) CHO cells demonstrated significantly enhanced recombinant antibody-specific productivity. The use of small-molecule SETDB1 inhibitors resulted in a comparable enhancement of transgene expression to that observed with SETDB1 silencing. During extended cultivation, recombinant protein production progressively diminished concurrent with declining SETDB1-KD efficacy. This temporal correlation demonstrates that SETDB1-mediated epigenetic regulation is essential for maintaining both recombinant protein titers and chromosomal stability in industrial biomanufacturing processes. Transcriptome dynamics analysis revealed that SETDB1 silencing induces transcriptional pattern remodeling in transfected cells. These findings elucidate SETDB1's regulatory role in CHO cells and provide actionable insights for the optimisation of recombinant protein production through cell engineering strategies.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"110"},"PeriodicalIF":3.7,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12297104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cucurbitacin B alleviates DSS-induced ulcerative colitis by improving gut microbiota disorder in C57BL/6 mice. 葫芦素B通过改善C57BL/6小鼠肠道菌群紊乱来缓解dss诱导的溃疡性结肠炎。

IF 3.7 3区生物学

AMB Express Pub Date : 2025-07-26 DOI: 10.1186/s13568-025-01922-5

Jichen Li, Xiaodi Shen, Xia Wu, Fan Zhao, Wenling Tang, Minglan Wu, Fang Hu, Xingjiang Hu, Fei Wang, Qiao Zhang

{"title":"Cucurbitacin B alleviates DSS-induced ulcerative colitis by improving gut microbiota disorder in C57BL/6 mice.","authors":"Jichen Li, Xiaodi Shen, Xia Wu, Fan Zhao, Wenling Tang, Minglan Wu, Fang Hu, Xingjiang Hu, Fei Wang, Qiao Zhang","doi":"10.1186/s13568-025-01922-5","DOIUrl":"10.1186/s13568-025-01922-5","url":null,"abstract":"Cucurbitacin B (CuB) is a triterpenoid compound derived from various medicinal plants, demonstrating potential anti-inflammatory, antioxidant, and neuroprotective properties, as well as significant anti-tumor effects. However, its efficacy in treating ulcerative colitis (UC) remains unclear. To investigate the therapeutic potential of CuB, a dextran sulfate sodium (DSS)-induced UC model in mice was employed, along with gut microbiota analysis. The results revealed that CuB significantly alleviated clinical symptoms, improved colonic tissue damage, and suppressed pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 in colon tissues. Additionally, CuB was associated with changes in specific microbial populations, including the upregulation of Muribaculaceae, Rikenellaceae_RC9_gut_group, Muribaculum, and Bifidobacterium, and the downregulation of Desulfovibrionaceae, Helicobacter, Escherichia-Shigella, Streptococcus, Candidatus_Saccharimonas, and Clostridium, which may contribute to the recovery of colon injury. This study provides preliminary evidence supporting CuB's therapeutic potential in DSS-induced colitis by enhancing gut microbiota diversity. CuB shows promise as potential treatment for UC and other conditions related to disruptions in intestinal flora homeostasis.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"111"},"PeriodicalIF":3.7,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12297160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Zalaria obscura Y1223 hydrolases: implications for lignocellulose conversion. 黑扎霉Y1223水解酶的表征：木质纤维素转化的意义。

IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-23 DOI: 10.1186/s13568-025-01907-4

Jeremy J Boonzaier, Rodney S Hart, Justin W Hoff, Heinrich W du Plessis, Riaan den Haan

{"title":"Characterization of Zalaria obscura Y1223 hydrolases: implications for lignocellulose conversion.","authors":"Jeremy J Boonzaier, Rodney S Hart, Justin W Hoff, Heinrich W du Plessis, Riaan den Haan","doi":"10.1186/s13568-025-01907-4","DOIUrl":"10.1186/s13568-025-01907-4","url":null,"abstract":"Overconsumption of fossil fuel reserves and its adverse effects has sparked interest in the production of second-generation biofuels due to the abundance of lignocellulosic waste and potential energy crops. However, processing costs associated with depolymerization of the cellulose crystalline structure have stalled advancement in cellulosic ethanol production. Current investigations range from identification of novel enzymes for lignocellulose hydrolysis to consolidation of enzyme production into a singular alcohol producing microorganism to potentially reduce cost for commercial processing. In this study, a total of 828 non-Saccharomyces and black yeasts were screened for cellulolytic and xylanolytic enzyme activities, whereby 60 isolates were identified that exhibited activity for at least one of the enzymes tested. In doing so, a novel Zalaria obscura strain (Z. obscura Y1223) was identified and assessed for enzyme activity in multiple growth media. Semi-quantitative assays showed that Z. obscura Y1223 produced cellulases optimally in media containing yeast extract, peptone and oat bran, with a pH range between pH 5 and 6 and at 30 °C. Maximum xylanase activity (20.5 U/L/OD600) was attained using synthetic complete media supplemented with xylo-oligosaccharides and maximum cellulase activity (7.51 U/L/OD600 endoglucanase, 1.302 U/L/OD600 β-glucosidase) was attained when grown in media containing yeast extract, peptone and oat bran. To our knowledge, this is the first study to quantify the cellulolytic and xylanolytic enzyme activities of a Zalaria spp., which provides key insight into the availability of unexplored cellulolytic enzymes that could inform the design of organisms engineered for consolidated bioprocessing.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"109"},"PeriodicalIF":3.5,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12287495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Milk yield, rumen fermentation, and microbiota of Shami goats fed diets supplemented with spirulina and yeast. 添加螺旋藻和酵母对沙米山羊产奶量、瘤胃发酵和微生物群的影响。

IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-21 DOI: 10.1186/s13568-025-01916-3

Alaa Emara Rabee, Moustafa Mohamed M Ghandour, Ahmed M Sallam, Osama Raef, Eman A Elwakeel, Ebrahim A Sabra, Adel M Abdel-Wahed, Salah Abo Bakr, Hanan Saad ElSamahy, Amal Amin Hamed, Mebarek Lamara

{"title":"Milk yield, rumen fermentation, and microbiota of Shami goats fed diets supplemented with spirulina and yeast.","authors":"Alaa Emara Rabee, Moustafa Mohamed M Ghandour, Ahmed M Sallam, Osama Raef, Eman A Elwakeel, Ebrahim A Sabra, Adel M Abdel-Wahed, Salah Abo Bakr, Hanan Saad ElSamahy, Amal Amin Hamed, Mebarek Lamara","doi":"10.1186/s13568-025-01916-3","DOIUrl":"10.1186/s13568-025-01916-3","url":null,"abstract":"Microalgae and live yeast have gained interest in improving animal performance. This study evaluated the effect of supplementation with Spirulina platensis, Saccharomyces cerevisiae, or their combination on the in vitro and in vivo rumen fermentation, rumen microbiota, and milk yield and composition of lactating Shami goats. The in vitro experiment included four diets: non-supplemented basal diet consisted of Alfalfa hay and a concentrate feed mixture (C); basal diet supplemented with 1% Saccharomyces (Y) based on dry matter; basal diet supplemented with 1% Spirulina (A); and basal diet supplemented with 1% of a mixture of Saccharomyces and Spirulina (AY). In the in vivo experiment, twenty-one lactating goats were divided into three groups (n = 7) to receive one of three diets: C, A, and AY. Group AY had higher in vitro gas production, dry matter digestion (DMD), and volatile fatty acids (VFA) (p < 0.05). Milk yield and feed efficiency were higher in groups A and AY compared to group C. Group AY goats exhibited higher rumen total VFA, acetic, and propionic, while group A showed higher butyric acid. Lower predicted methane was observed in group AY. Groups A and AY showed distinctive microbial communities. The bacterial community was dominated by phylum Bacteroidota, and genera Prevotella and Rikenellaceae RC9 gut group, which were higher in the AY group. The archaeal community was dominated by the genus Methanobrevibacter, which had a lower prevalence in group AY. The combination of live yeast and Spirulina improved rumen fermentation and the milk yield; therefore, it could be used as a feed additive for lactating goats.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"108"},"PeriodicalIF":3.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12279650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin-conjugated magnetic nanoparticles inhibit Staphylococcus aureus growth and biofilm formation via downregulation of Coa and Hla genes. 槲皮素偶联磁性纳米颗粒通过下调辅酶a和Hla基因抑制金黄色葡萄球菌生长和生物膜形成。

IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-15 DOI: 10.1186/s13568-025-01915-4

Alireza Habibi, Masoumeh Pakpour Roudsari, Seyyed Mahdi ZiaZiabari

{"title":"Quercetin-conjugated magnetic nanoparticles inhibit Staphylococcus aureus growth and biofilm formation via downregulation of Coa and Hla genes.","authors":"Alireza Habibi, Masoumeh Pakpour Roudsari, Seyyed Mahdi ZiaZiabari","doi":"10.1186/s13568-025-01915-4","DOIUrl":"10.1186/s13568-025-01915-4","url":null,"abstract":"The research investigated the effects of that magnetic nanoparticles coated with the plant-based flavonoid quercetin have on the growth of Staphylococcus aureus. The synthesis of magnetic nanoparticles proceeded through co-precipitation and further involved quercetin coating using a dextran stabilizer. A combined product of these materials received the chemical name Fe3O4@Dex-QT. The study included multiple analytical tests such as FTIR (Fourier Transform Infrared Spectroscopy), FE-SEM (Field Emission Scanning Electron Microscopy), EDS (Energy-Dispersive X-Ray Spectroscopy) and XRD (X-ray diffraction) together with cellular and molecular assays. Biofilm detection experiments showed that 95% of studied microorganisms had moderate-to-strong biofilm formation abilities. The examined strains that formed biofilms exhibited complete biofilm inhibition at a minimum concentration of 512 μg/mL. Post-treatment with Fe3O4@Dex-QT nanoparticles, the Coa and Hla genes were expressed at 30 and 20% levels respectively, as determined through RT-PCR analysis. The research uncovered that Fe3O4@Dex-QT nanoparticles demonstrate successful antibacterial properties against S. aureus strains, making them viable for substituting conventional antibiotics.","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"107"},"PeriodicalIF":3.5,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12263539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mendelian randomization analysis of gut microbiota-immune cell interactions in malignant neoplasm of nasopharynx. 鼻咽部恶性肿瘤中肠道微生物-免疫细胞相互作用的孟德尔随机分析。

IF 3.5 3区生物学

AMB Express Pub Date : 2025-07-15 DOI: 10.1186/s13568-025-01909-2

Qicong Chen, Gang Wang, Jingcheng Shu, Xiaosu Zou, Weiwei Miao, Wenqian Nong, Min Li, Guiping Lan, Wenlin Huang, Xueying Huang, Honglin Luo, Shenhong Qu

{"title":"Mendelian randomization analysis of gut microbiota-immune cell interactions in malignant neoplasm of nasopharynx.","authors":"Qicong Chen, Gang Wang, Jingcheng Shu, Xiaosu Zou, Weiwei Miao, Wenqian Nong, Min Li, Guiping Lan, Wenlin Huang, Xueying Huang, Honglin Luo, Shenhong Qu","doi":"10.1186/s13568-025-01909-2","DOIUrl":"10.1186/s13568-025-01909-2","url":null,"abstract":"Observational studies have suggested associations among the gut microbiome, immune cells, and the risk of malignant neoplasms of nasopharynx. However, the causality of these relationships remains unclear. Thus, we conducted multiple Mendelian Randomization analyses to estimate the causal association of gut microbiota with the risk of malignant neoplasms of nasopharynx and to evaluate the mediating effect of immune cells on this causal pathway. Genetic variants extracted from genome-wide association studies of human gut microbiota compositions (n = 211), immune cell traits (n = 731) and malignant neoplasms of nasopharynx served as instrumental variables for calculating causal associations and mediating effects. Four gut microbiota compositions and eight immune cell traits exhibited detrimental causal effects, while three gut microbiota compositions and fifteen immune cell traits demonstrated protective effects. Interestingly, the causal association of genus Candidatus Soleaferrea id.11350 was no longer significant after adjusting for two established immune cell traits (HLA DR + + monocyte %leukocyte and HLA DR + + monocyte % monocyte). Moreover, HLA DR + + monocyte %leukocyte exhibited a mediating effect (OR 0.75, 95% CI 0.59-0.96) on the causal pathway of genus Candidatus Soleaferrea id.11350-malignant neoplasms of nasopharynx, with a mediating proportion of 21.59%. To our knowledge, this study is the first to identify potential therapeutic targets and elucidate mechanistic insights for malignant neoplasms of nasopharynx interventions involving gut microbiota and immune cell traits; however, these findings warrant further validation through adequately powered randomized clinical trials (RCTs).","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"106"},"PeriodicalIF":3.5,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12263519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0