AMB Express最新文献

{"title":"In vitro and in vivo antitumor activity of Laz-expressing Salmonella Typhimurium YB1 via optimized secretion and regulation.","authors":"Yahia I Abouelenen, Radwa M Ramzy, Alshaymaa Amin Zaki El-Bahy, Christian U Riedel, Khaled M Abou-Aisha, Manar M Mansour","doi":"10.1186/s13568-026-02057-x","DOIUrl":"10.1186/s13568-026-02057-x","url":null,"abstract":"<p><p>Salmonella enterica serovar Typhimurium strain YB1 is a genetically engineered obligate anaerobe with strong tumor-targeting potential. In this study, we evaluated YB1 as a delivery platform for Laz, a lipidated azurin-derived protein, using three secretion strategies: a native lipoprotein signal peptide (SPase II; YDL), a Sec-dependent secretory signal peptide (SPase I; YDN), and a Type III secretion signal (YDP). Laz expression was controlled by the aTc-inducible Pxyl/tet promoter on a pET29a backbone. To eliminate the confounding effects of metabolic burden, we constructed a TetR-matched control plasmid (named YC*) to be used as a comparator. Compared with YC*, Laz-expressing YB1 strains significantly reduced the viability of MCF-7 cells: YDL (50.69 ± 5.51%, p < 0.001), YDN (52.05 ± 4.69%, p < 0.001), and YDP (51.03 ± 3.92%, p < 0.001). This effect was dependent on induction conditions, with stronger responses observed at higher aTc concentrations. In vivo evaluation using the Ehrlich carcinoma mouse model revealed increased tumor necrosis and reduced mitotic activity in bacterially-treated groups. Among the constructs, YDL exhibited higher Laz expression and export, which was associated with more pronounced effects in vitro and consistent trends in vivo. Collectively, these findings support the feasibility of YB1-mediated Laz delivery as a bacterial delivery platform and highlight the importance of matched regulatory architecture in the design of engineered bacterial therapeutics. This study provides a functional evaluation of Laz-associated cytotoxic effects in the YB1 system while indicating areas for further investigation.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"16 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13179408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147947461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis and application of microbiota in fermentation pit muds used for Chinese strong-flavor liquor production.","authors":"Hong Dong, Qingyi Fu, Qingyang Wan, Bin Li, Wanxiang Zhang, Xunduan Huang, Xingjie Chen, Laoji Yang, Bing Peng, Guopai Xie, Hongwen Yang, Buchang Zhang, Yansheng Wang","doi":"10.1186/s13568-026-02070-0","DOIUrl":"10.1186/s13568-026-02070-0","url":null,"abstract":"<p><p>Chinese strong-flavor liquor (CSFL), the primary type of Chinese liquor in terms of both production and sales, is produced through complex fermentation in pits covered with fermentation pit mud (FPM). FPM, which contains complex microbiota, is crucial for the quality of CSFL. Therefore, monitoring the quality of FPM and acquiring high-quality FPM are important for CSFL manufacturers. However, this requires further study because the relationship between the microbiota and FPM quality remains largely unclear. In this study, high- and low-quality FPM samples were selected based on two-year production data, and the microbiota were sequenced with the HiSeq2500 PE250 platform. Compared with low-quality FPMs, high-quality FPMs exhibited more similar microbiota, and a higher abundance of Euryarchaeota and Firmicutes. Furthermore, the relative abundance of five abundant genera including Methanobacterium, Methanosaeta, Clostridium, Syntrophomonas and Aminobacterium, were positively correlated with the quality of FPM and could construct a synergistic network helpful for inhabiting FPM and improving the quality of CSFL. At the species level, the abundance of Clostridium kluyveri and the new biomarker Syntrophomonas wolfei increased in high-quality FPMs and could be rapidly detected using polymerase chain reaction (PCR). By applying functional microbes and knowledge of the microbial ecosystem, artificial FPM was successfully incubated within 50 days, and the yield of high-quality CSFL had an increase of 43.72% compared to that produced with mature FPM. This study provides new insights into further understanding of the microbial ecosystem of FPM and will be useful for producing artificial FPM, as well as for monitoring and improving the quality of FPM used for CSFL production.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13341992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147939540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial assembly and metabolic restructuring following CO₂ injection in subsurface oil reservoir.","authors":"Le-Gang Chen, Lei Zhou, Bo-Wen Wang, Muhammad Javed, Yi-Fan Liu, Shi-Zhong Yang, Ji-Dong Gu, Bo-Zhong Mu","doi":"10.1186/s13568-026-02066-w","DOIUrl":"10.1186/s13568-026-02066-w","url":null,"abstract":"<p><p>Carbon dioxide (CO₂) injection into oil reservoirs is a well-established and promising strategy for enhancing oil recovery while simultaneously sequestering carbon, whose effectiveness is increasingly recognized to be influenced by microbial-mediated mechanisms and biogeochemical processes. However, the impact of CO₂-injected and storage on functional microbial communities and their metabolic potential remains poorly understood. The study employed high-throughput sequencing to analyze the composition and diversity of microbial communities in produced water from three production wells with a long-term (> 20 years) history of CO₂ injection and two adjacent water-flooding ones in Xinli Oilfield, China. The results showed that CO₂-injected wells had significantly higher microbial diversity and distinct metabolic potential relative to the adjacent water-flooding wells. A metabolic difference in the archaeal communities towards hydrogenotrophic and heterotrophic metabolisms, alongside an enrichment of bacterial taxa associated with sulfur and nitrogen cycling was observed. Correlation analysis revealed that microbial differentiation was strong associated with geochemical alteration (especially pH and some inorganic ions), with NH₄⁺ and S^2- identified as key factors in this process. Metagenomic analysis based on high-quality metagenome-assembled genomes (MAGs) further demonstrated that CO₂ injection led to a different profile of microbial metabolic functions relative to the water-flooding condition, characterized by significantly enhancing hydrogenotrophic methanogenesis, dissimilatory sulfate reduction, and denitrification, while diminishing methylotrophic methanogenesis and Wood-Ljungdahl pathway activity. These findings provide new insights into the microbial mechanisms driving carbon transformation in CO₂-flooded oil reservoirs.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13342004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147925668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance among Gram-negative bacteria in bloodstream infections in Shiraz, Iran (2012-2023).","authors":"Mohammad Hossein Nejati Boor Boor, Sahar Sabour, Hadi Ghasemi, Neda Mohagheghzadeh, Hossein Safari, Fatemeh Toranjzard, Zahra Esfandiari, Kosar Choopan, Shiva Nazari, Saeed Soleiman Meigooni, Taher Azimi","doi":"10.1186/s13568-026-02061-1","DOIUrl":"10.1186/s13568-026-02061-1","url":null,"abstract":"<p><p>Regular monitoring and conducting surveillance studies are necessary to control and treat antibiotic-resistant infections on a global scale. The present study aimed to perform a time-trend analysis of the distribution and antibiotic resistance of Gram-negative bacteria (GNB) isolated from bloodstream infections over 12 years in Iran. Throughout a 12-year duration, from January 2012 until the end of December 2023, data from the blood specimens of patients were obtained from three university teaching hospitals in Shiraz, Iran. Antimicrobial susceptibility profiles of GNB were determined using the Kirby-Bauer disk diffusion method (DDM) on Mueller-Hinton agar (MHA) media. A total of 391,762 blood samples were obtained from patients. Among these, 40,397 (10.31%) were confirmed to be positive for GNB. The frequency of GNB was higher among patients aged over 50 years. The highest number of GNB was isolated from the emergency (50.54%) ward. Escherichia coli with 42.65%, and Klebsiella spp. with 17.02% frequently isolated from blood specimens. CAZ was the most effective antimicrobial agent against Pseudomonas aeruginosa (7.16%), Acinetobacter spp. (1.18%), E. coli and Klebsiella spp. (1.17%). The trends observed in antibiotic resistance among GNB from 2012 to 2023 indicate a reduction in resistance levels for certain antibiotics: SXT decreased from 75.41 to 58.85%, TET from 82.39 to 25%, CAZ from 85.21 to 42.86%, AMP from 92.14 to 62.5%, and FEP from 100 to 67.96%. Results demonstrate a general downward trend in antibiotic resistance among GNB. However, variation in antibiotic resistance profile among GNB suggested that follow-up antimicrobial resistance (AMR) programs, along with constant screening and developing antimicrobial stewardship programs in Shiraz, are necessary.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13341989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147873040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary zinc fulvate supplementation on egg quality, serum biochemical indices, and intestinal health of aged laying hens.","authors":"Kai Guo, Yanling Feng, Genwang Xu, Tao Yu, Kai Zhang, Rui Zhao, Bochi Zhang, Liying Du, Chunlei Yang, Xianyi Song","doi":"10.1186/s13568-026-02065-x","DOIUrl":"10.1186/s13568-026-02065-x","url":null,"abstract":"<p><p>Zinc fulvate (ZFA), a chelate of fulvic acid and zinc, exhibits superior bioavailability and multifunctional properties that benefit animal health. This study aimed to evaluate the effects of dietary ZFA supplementation at different levels on egg quality, serum biochemical indices, intestinal morphology, and cecal microbiota composition in aged laying hens. A total of 480 Hy-Line Gray laying hens (63 weeks old) were randomly assigned to four groups with eight replicates each (15 hens per replicate): a control group (CON) fed a basal diet, and three experimental groups supplemented with 600 mg/kg (ZFA 600), 1200 mg/kg (ZFA 1200), and 1800 mg/kg (ZFA 1800) of ZFA, respectively. The experiment lasted for 8 weeks after a 2 weeks adaptation period. The results revealed that: (1) Compared with the CON group, ZFA 600 and ZFA 1200 groups significantly increased eggshell thickness and strength (p < 0.05). (2) ZFA 1200 group had significantly higher serum copper-zinc superoxide dismutase (CuZn-SOD) activity and lower malondialdehyde (MDA) content (p < 0.05), while ZFA 600, ZFA 1200, and ZFA 1800 groups all showed elevated serum albumin (ALB) levels (p < 0.05). (3) ZFA 600 group significantly increased villus height (VH) in the duodenum, jejunum, and ileum, as well as the villus height-to-crypt depth ratio (VH/CD) in the duodenum and jejunum (p < 0.05), while ZFA 1200 group reduced crypt depth (CD) in the duodenum and jejunum (p < 0.05). (4) At the microbial level, ZFA 600 group significantly improved the Simpson and Shannon indices of cecal microbiota (p < 0.05), and increased the relative abundances of beneficial taxa including Firmicutes (Firmicutes_A, Firmicutes_B, Firmicutes_D), Actinobacteriota, Verrucomicrobiota at the phylum level, and Mediterraneibacter_A and Prevotella at the genus level (p < 0.05). Collectively, dietary ZFA supplementation improves eggshell quality, enhances antioxidant capacity, optimizes intestinal morphology, and modulates gut microbiota homeostasis in aged laying hens. Different ZFA doses have distinct advantage targets, and the suitable dose can be selected according to actual breeding needs, e.g., 600 mg/kg ZFA can be used as a recommended addition dose for balancing the overall cecal health of laying hens, while 1200 mg/kg ZFA is suitable for scenarios requiring focused alleviation of oxidative stress. Under the conditions of this study, 600 mg/kg ZFA is recommended as the optimal supplemental level based on overall beneficial effects and physiological rationality. However, the potential risk of excessive zinc intake at high ZFA doses was not fully considered in the present study, and lower doses of ZFA should be further evaluated in future research to meet nutritional standards more precisely.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13323435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147855795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial cultures optimize rhizosphere bacterial community in Phyllostachys edulis forests.","authors":"Zongsheng Yuan, Sifan Wang, Xiaoling Wang, Fang Liu","doi":"10.1186/s13568-026-02064-y","DOIUrl":"10.1186/s13568-026-02064-y","url":null,"abstract":"<p><p>This study examined the impact of endophytic bacterial cultures, isolated from Phyllostachys edulis, on the bacterial communities within the P. edulis rhizome roots and rhizosphere, as well as on soil chemical properties. The mixed microbial cultures consisting of four strains was applied to experimental plots (low-yield forests). Seven rhizome roots samples were collected from P. edulis shoots at 0, 3, and 6 months: both treated and untreated samples were included at each time point. Similarly, seven rhizosphere soil samples were taken from P. edulis shoots at the same intervals, with treated and untreated samples at each time point. These samples were analyzed to assess changes in the rhizome roots endophytic and rhizosphere bacterial communities, as well as shifts in soil nutrient availability and chemical properties. The results indicated that after rarefaction to 93,259 sequences per sample, OTU-based (97% similarity) and Hellinger-transformed analyses revealed that treated samples showed increased bacterial richness (145-229) and Firmicutes abundance (1.9-11.1%) over six months, with directional shifts in community composition toward high-yield reference characteristics. However, Shannon diversity declined moderately (4.24 → 3.65) and remained below reference levels (p < 0.05). RDA identified EC, AP, TP, TK, AK, and AN as significant drivers (r² = 0.408-0.679, p < 0.05). The diversity of endophytic bacteria in P. edulis rhizome roots increased with the duration of microbial cultures application. The relative abundance of Firmicutes was notably influenced by the treatment. These findings demonstrate that microbial cultures positively influence the bacterial communities in both the rhizome roots and rhizosphere soil of P. edulis. Manipulating the bacterial community in P. edulis forest soils through the application of carefully selected beneficial strains appears to be a viable strategy.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13319836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly Cu-TiO₂ nanoparticles from Citrus limon peel: integrated biological and computational evaluation.","authors":"Hansa Gul, Zahida Nasreen, Muhammad Nauman Khan, Maged Mostafa Mahmoud, Mohammed S Almuhayawi, Shadi A Zakai, Tewekel Melese Gemechu, Muhammad Adnan, Alevcan Kaplan","doi":"10.1186/s13568-026-02060-2","DOIUrl":"10.1186/s13568-026-02060-2","url":null,"abstract":"<p><p>The green synthesis of multifunctional nanomaterials from agricultural waste is a major target in sustainable biomedicine. This study reports the eco-friendly, one-pot synthesis of copper-doped titanium dioxide nanoparticles (Cu-TiO₂NPs) using an aqueous peel extract of Citrus limon (Linn.) Burm. f. The formation of the nanoparticles was observed using the ultraviolet-visible spectroscopy, with an absorption edge below 400 nm with a redshift in the visible range (400-650 nm). X-ray diffraction (XRD) confirmed a mixed anatase-rutile crystalline phase, with an average crystallite size of 45.6 nm, and scanning electron microscopy (SEM) showed irregularly shaped agglomerated nanoparticles with an average diameter of 48 ± 0.46 nm. Field Emission Scanning Electron Microscopy (FESEM) revealed irregular, highly agglomerated nanoparticles with a primary size range of 49 ± 07 nm, while X-ray Photoelectron Spectroscopy (XPS) confirmed the oxidation states of Ti, O, and Cu, validating successful doping. Energy-Dispersive X-ray (EDX) analysis further confirmed uniform Cu incorporation into the TiO₂ lattice. In vitro testing showed a dose-dependent antibacterial effect against Staphylococcus aureus (ATCC 9144), Staphylococcus epidermidis (ATCC 12228), and Pseudomonas aeruginosa (ATCC 10145), with minimum inhibitory concentration (MIC) values ranging from 15 to 20 µg/mL. The highest zone of inhibition for Cu-TiO₂NPs was 21.00 ± 0.29 mm against S. epidermidis at 30 µg/mL, similar to the effect observed with ceftriaxone (30 µg/mL). The nanoparticles exhibited high antioxidant capacity, with a ferric reducing antioxidant potential of 67.5 ascorbic acid equivalents (AAE) µg/mL and a 54% 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity at 500 µg/mL. Cytotoxicity was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, which indicated dose-dependent cytotoxicity in human hepatocellular carcinoma (HepG2) cells, with an IC₅₀ of 150 µg/mL after 24 h. The stabilization of the L-ascorbic acid-Cu-TiO₂NPs complex was investigated using Density Functional Theory (DFT), and molecular docking revealed strong interactions with the target bacteria. This study uniquely integrates sustainable waste valorization, comprehensive in vitro bio evaluation, and computational validation of lemon peel-derived Cu-TiO₂NPs for biomedical applications.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13315372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Kaempferol ameliorates Cisplatin induced nephrotoxicity by modulating oxidative stress, inflammation and apoptosis via ERK and NF-κB pathways.","authors":"Zhu Wang, Wansen Sun, Xi Sun, Ye Wang, Meilan Zhou","doi":"10.1186/s13568-026-02059-9","DOIUrl":"10.1186/s13568-026-02059-9","url":null,"abstract":"","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"16 1","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brucella abortus outer membrane protein modulates host immunity via B-cell receptor-associated protein.","authors":"Yasamin Nasiri Khonsari, Reza Habibi, Fatemeh Alami, Rana Mahmoud Mustafa, Mahsa Manafi Varkiani, Armita Banimahdidehkordi, Mehrshad Fekri, Hossein Bakhtar Asl, Mona Moosavi, Ehsan Heidari-Soureshjani","doi":"10.1186/s13568-026-02042-4","DOIUrl":"10.1186/s13568-026-02042-4","url":null,"abstract":"<p><p>Brucella outer membrane protein 10 (OMP10) plays a critical role in host–pathogen interactions, yet its molecular mechanisms in modulating inflammatory and apoptotic pathways remain unclear. Here, we combined in vivo transcriptional profiling with computational analyses to investigate OMP10 function. Oral delivery of OMP10-expressing Lactococcus lactis in mice induced significant upregulation of BCAP31, CASP8, and IL1B, indicating engagement of ER stress, apoptotic, and NF-κB–mediated inflammatory responses. Protein–protein interaction network analysis across STRING confidence thresholds (0.4, 0.7, 0.9) highlighted CASP8 and IL1B as central hubs, with BCAP31 and PACS2 as key intermediates. Sequence-based docking and molecular dynamics simulations identified stable, energetically favorable interactions between OMP10 and BCAP31, PACS2, and TNFSF10, consistent with modulation of ER–MAM signaling and caspase-8 priming. Functional enrichment linked these interactions to unfolded protein response, CASP8 activation, and IL-1β production. Collectively, these results reveal OMP10 as a multifunctional Brucella effector that orchestrates ER stress–inflammation–apoptosis crosstalk and suggest that targeting the OMP10–BCAP31 axis could inform novel therapeutic or vaccine strategies against brucellosis.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13247013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota metabolites drive osteosarcoma progression through JUP activation: a multi-omics mendelian randomization study.","authors":"Qun Zhang, Tianran Li","doi":"10.1186/s13568-026-02056-y","DOIUrl":"10.1186/s13568-026-02056-y","url":null,"abstract":"<p><p>Osteosarcoma exhibits high heterogeneity and lacks effective therapeutic targets. This study integrated Mendelian randomization, transcriptomics, and 16S rRNA sequencing to investigate potential associations between gut microbiota, metabolites, and osteosarcoma. Multi-omics analysis identified junction plakoglobin (JUP) as a candidate node gene. 16S rRNA sequencing revealed reduced abundance of bacteria belonging to the genus Lactobacillus in osteosarcoma patients. Based on database-derived associations from gutMGene, we selected a Lactobacillus-associated metabolite, 5-(3,4-dihydroxyphenyl)-valerolactone (DPV), for molecular docking analysis, which computationally predicted that DPV may bind JUP, though this finding remains to be experimentally validated. JUP expression positively correlated with M0 macrophage infiltration, suggesting a potential link to the immune microenvironment. To explore this axis experimentally, we established JUP-knockdown osteosarcoma cells and examined responses to Lactiplantibacillus plantarum and DPV. Both treatments significantly inhibited proliferation, colony formation, and invasion in JUP-expressing cells, whereas these effects were attenuated in JUP-knockdown cells, suggesting that JUP may play a role. Overall, this study provides a working hypothesis-generating framework linking gut microbiota, microbial metabolites, and JUP in osteosarcoma, presenting this connection as a testable hypothesis rather than an established biological axis. The multi-omics datasets were analyzed separately without formal integrative modeling; therefore, this study is exploratory and the findings should be interpreted as hypothesis-generating. These findings suggest directions for further investigation into microbiota-related strategies in osteosarcoma.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13260498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}