抗K1肺炎克雷伯菌噬菌体Henu2_3的鉴定、基因组学及动物模型疗效研究

IF 3.7 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Qiming Li, Jiaqi Li, Yanyang Zhao, Shuai Guo, Mengzhe Liu, Xiaoyu Shi, Li Wang, Zhigang Liu, Tieshan Teng
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引用次数: 0

摘要

多药耐药和超耐药菌株的出现和传播使噬菌体治疗成为未来感染治疗的一种非常有前途的方法。肺炎克雷伯菌是临床感染和医院获得性疾病的主要原因,是克雷伯菌属中95%以上感染的原因。针对肺炎克雷伯菌的噬菌体表现出显著的多样性,有许多分离物被鉴定出对抗这种病原体的不同血清型。在这项研究中,我们从医院污水中分离并鉴定了一种新的噬菌体Henu2_3,该噬菌体针对临床分离的k1型肺炎克雷伯菌。透射电镜显示噬菌体Henu2_3具有二十面体头部和足病毒形态。噬菌体基因组包含42,878个碱基对,G + C含量为53.97%,编码54个假定的开放阅读框。一步生长曲线分析表明,噬菌体Henu2_3的潜伏期为10 min,平均每个感染细胞爆发215个噬菌体颗粒。此外,Henu2_3表现出显著的稳定性,可以耐受高达60℃的温度,并在4至12的广泛pH范围内保持最大的活力。体内感染模型表明,噬菌体Henu2_3可显著提高动物存活率,降低靶器官细菌负荷。这些特性突出了其作为治疗肺炎克雷伯菌感染的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization and genomics of phage Henu2_3 against K1 Klebsiella pneumoniae and its efficacy in animal models.

The emergence and spread of multidrug-resistant and super-resistant bacterial strains have positioned phage therapy as a highly promising approach for future infection treatments. Klebsiella pneumoniae, a leading cause of clinical infections and hospital-acquired diseases, is responsible for over 95% of infections within the Klebsiella genus. Phages targeting K. pneumoniae exhibit remarkable diversity, with numerous isolates identified against various serotypes of this pathogen. In this study, we described the isolation and characterization of a novel bacteriophage from hospital sewage, Henu2_3, which targeted clinical isolates of K1-type K. pneumoniae. Transmission electron microscopy revealed that phage Henu2_3 possessed an icosahedral head and podovirus morphotype. The phage genome comprises 42,878 base pairs with a G + C content of 53.97%, encoding 54 putative open reading frames. One-step growth curve analysis demonstrated that phage Henu2_3 has a latency period of 10 min and an average burst size of 215 phage particles per infected cell. Additionally, Henu2_3 exhibited remarkable stability, tolerating temperatures up to 60℃ and maintaining maximum viability across a broad pH range of 4 to 12. In vivo infection models demonstrated that phage Henu2_3 markedly enhanced animal survival and decreased bacterial burden in target organs. These properties highlight its potential as a therapeutic agent against K. pneumoniae infections.

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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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