AMB Express最新文献

{"title":"Expediting multiple biological properties of main bioactive compounds of Mentha pulegium L.","authors":"Nasreddine El Omari, Oumayma Aguerd, Abdelaali Balahbib, Naoual El Menyiy, Mohammed Amanullah, Jactty Chew, Long Chiau Ming, Abdelhakim Bouyahya","doi":"10.1186/s13568-025-01911-8","DOIUrl":"10.1186/s13568-025-01911-8","url":null,"abstract":"<p><p>This study aimed to evaluate the antidiabetic, dermatoprotective, and antibacterial properties of Mentha pulegium L. essential oil (MPEO), harvested in Ouezzane, northwest Morocco. Gas chromatography-mass spectrometry (GC-MS) analysis identified pulegone (41.0%) and menthone (21.2%) as the major constituents. The antibacterial activity was assessed using the micro-broth dilution method, while antidiabetic and dermatoprotective effects were evaluated via in vitro inhibition of α-amylase, α-glucosidase, tyrosinase, and elastase. Additionally, molecular docking was used to assess interactions between key enzymes and major compounds. Both menthone and pulegone exhibited strong antibacterial effects against Staphylococcus aureus and Listeria monocytogenes, with MIC and MBC values ranging from 0.125 to 0.25 µg/mL. Remarkably, menthone and pulegone demonstrated MIC = MBC = 0.125 µg/mL against S. aureus and L. monocytogenes, respectively. In antidiabetic assays, all tested compounds outperformed acarbose. Menthone showed the best inhibition with IC₅₀ values of 149.32 ± 4.16 and 108.39 ± 4.08 μg/mL for α-amylase and α-glucosidase, respectively. MPEO also displayed potent dermatoprotective activity, with IC₅₀ values of 56.23 ± 2.24 μg/mL (tyrosinase) and 90.36 ± 2.26 μg/mL (elastase), compared to quercetin. In silico analysis confirmed strong binding affinities of pulegone and menthone to target enzymes involved in glucose regulation. These findings highlight MPEO as a promising natural source of bioactive compounds with antibacterial, antidiabetic, and dermatoprotective properties, supporting its potential use in pharmaceutical and therapeutic applications.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"105"},"PeriodicalIF":3.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12259508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design a multi-epitope vaccine candidate against Acinetobacter baumannii using advanced computational methods.","authors":"Hana Heidarinia, Elahe Tajbakhsh, Yadollah Bahrami, Mosayeb Rostamian","doi":"10.1186/s13568-025-01913-6","DOIUrl":"10.1186/s13568-025-01913-6","url":null,"abstract":"<p><p>Acinetobacter baumannii is a significant cause of hospital-acquired infections and is often resistant to multiple antibiotics. In this study, we designed a multi-epitope vaccine candidate using the outer membrane protein K (OmpK) of A. baumannii. T-cell and B-cell epitopes were predicted, and the best epitopes were selected for multi-epitope design. For selecting the best epitopes, many in silico studies, as well as molecular docking of epitope-HLAs, were performed. The multi-epitope was designed using β-defensin as an adjuvant, PADRE sequence as an immunogenicity enhancer, and appropriate linkers. The tertiary structure of the multi-epitope was obtained using modeling and several molecular dynamics (MD)-based refinements. The sequence and/or 3D model of the multi-epitope was investigated for physicochemical, structural, in silico cloning, conformational B epitope prediction, immune response simulation, molecular docking for assay binding to toll-like receptors (TLRs), and deformability studies. The results showed that the multi-epitope construct is favorable in the case of immunogenicity, physicochemical properties, structure, binding to TLRs, solubility, stability, toxicity, allergenicity, and cross-reactivity. This multi-epitope vaccine candidate has the potential to elicit multiple immune responses against A. baumannii. However, in vitro and in vivo experimental tests are needed to validate its efficacy as a potential vaccine candidate.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"103"},"PeriodicalIF":3.5,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking Reishi's secrets: nutritional and medicinal traits of Ganoderma lucidum isolated from tree bark in Egypt.","authors":"Ramy M Shady, Mona H El-Gayar, Eman El-Gebaly, Hatem S Abbass, Mahmoud A Yassein","doi":"10.1186/s13568-025-01905-6","DOIUrl":"10.1186/s13568-025-01905-6","url":null,"abstract":"<p><p>Ganoderma lucidum is an edible mushroom with high consumption rates in many cultures especially East Asian. This study assessed the in-vitro antimicrobial, antitumor, antiviral as well as antioxidant capacity of Ganoderma lucidum water extract (GLWE). Different microorganisms (standard strain bacteria and clinical isolates) were affected by mushroom aqueous extract, with inhibition zone diameters ranging between 16.1 and 38.8 mm. The MTT assay was used to evaluate antitumor efficacy against three distinct tumor cell lines: HepG-2 (liver tumor cell line), MCF-7 (breast tumor cell line), in addition to Caco2 (colorectal carcinoma cell line). GLWE was found to be safe for Vero cells (CC₅₀ 5237 ± 1.6 µg/mL) and had detectable anticancer efficacy against all tested cell lines with IC₅₀ ranges from 99.06 to 198.1 µg/mL. The Petricevich and Mendonça technique was used to test the antiviral activity against herpes simplex virus-1 together with Human adeno virus-7. The findings demonstrated modest antiviral activity, with EC₅₀ values of 493.8 ± 0.98 and 607.1 ± 1.9 µg/mL and corresponding selectivity indices of 10.6 and 8.62, respectively. On studying the antioxidant capacity of GLWE by using DPPH and ABTS, a potential activity with IC₅₀ values of 23.69 ± 0.85 and 14.79 ± 0.92 µg /mL, respectively, were obtained. Liquid chromatography-mass spectrometry in addition to high-performance liquid chromatography analysis indicated a diverse range of compounds including Ganoderic acids, Lucidenic acids, Ganolucidic acids, and Ganoderenic acids which are characteristic triterpenoids to the genus Ganoderma, as well as some polyphenols and flavonoids. In addition, some water/fat soluble vitamins were also detected, as well as proteins and carbohydrates contributing to the high nutritional value of the studied mushroom.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"104"},"PeriodicalIF":3.5,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of live attenuated and killed tachyzoites as vaccine candidates for toxoplasmosis.","authors":"Eman E El Shanawany, Eman H Abdel-Rahman, Waleed A Nemr, Soad E Hassan, Noha M F Hassan, Hassan M Desouky, Rabab Zalat, Amany Ebrahim Nofal, Raafat M Shaapan, Salwa Sami Younis","doi":"10.1186/s13568-025-01889-3","DOIUrl":"10.1186/s13568-025-01889-3","url":null,"abstract":"","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"102"},"PeriodicalIF":3.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring complete ammonia oxidizers bacteria: relevant players for nitrogen removal from wastewater.","authors":"Javier Duque, Leire Besga-Oyanarte, Miguel De Celis, Susana Serrano, José Luis Alonso, Antonio Santos, Lucía Arregui","doi":"10.1186/s13568-025-01878-6","DOIUrl":"10.1186/s13568-025-01878-6","url":null,"abstract":"<p><p>COMAMMOX (Complete Ammonia Oxidizers) bacteria seem to play an important role in nitrification, being able to directly transform ammonia into nitrates. Due to the relevance of this process in WWTPs, different approaches were used in this work to monitor COMAMMOX bacteria, as well as other microorganisms involved in the biological removal of nitrogen occurring in the bioreactor of a full-scale WWTP. This facility operates with low dissolved oxygen concentrations and prolonged sludge retention times. Fluorescence in situ hybridization confirmed the presence of COMAMMOX bacteria showing their distribution within the activated sludge flocs. Primers targeting the amoA gene, which encodes the A subunit of the ammonia monooxygenase enzyme, were employed as genetic markers in PCR techniques to simultaneously detect and amplify both COMAMMOX clades A and B, as well as specifically clade A, clade B, or various COMAMMOX Nitrospira species (Ca. N. nitrosa, Ca. N. inopinata, and Ca. N. nitrificans). Using digital PCR, a set of these primers enabled the estimation of the proportion of amoA COMAMMOX genes versus other nitrifiers (AOB, AOA and NOB). The data obtained not only confirmed the presence of COMAMMOX bacteria, but also their abundance, comparable to that of NOB and AOB. The 16S rRNA gene-based meta-taxonomy analysis gave complementary data about the microbial diversity and community composition within the samples. The results also highlight the biases of each technology used as monitoring tools, which must be accurate for the effective management of wastewater treatment plants (WWTPs) and for exploiting the potential of COMAMMOX bacteria.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"101"},"PeriodicalIF":3.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic evidence for causal links between diet, gut microbiota, and hepatobiliary cancer: a Mendelian randomization study.","authors":"Runze Huang, Xin Jin, Qinyu Liu, Xuanci Bai, Yibin Wu, Yixiu Wang, Xigan He, Ziting Jiang, Lu Wang, Weiping Zhu","doi":"10.1186/s13568-025-01910-9","DOIUrl":"10.1186/s13568-025-01910-9","url":null,"abstract":"<p><p>Emerging evidence suggests a complex interplay among dietary habits, gut microbiota, and hepatobiliary cancers, yet the causal relationships remain unclear. Here, we conducted a comprehensive two-sample Mendelian randomization (MR) analysis using genetic instruments from large European cohorts to assess causality among 88 dietary components, 1080 microbiota traits, liver cancer (500 cases, 314,193 controls), and biliary tract cancer (1207 cases, 314,193 controls). We identified significant causal associations of 17 dietary and 101 microbial traits with hepatobiliary cancer risk, while 11 dietary and 70 microbiota traits showed evidence of reverse causality, indicating potential disease-driven alterations. Importantly, a two-step MR mediation analysis revealed that 43 microbial taxa and 6 metabolic pathways significantly mediated dietary influences on hepatobiliary cancer risk; notably, Mollicutes RF9 mediated 31% of the protective effect exerted by zinc on biliary tract cancer. These findings provide genetic evidence delineating gut microbiota as key intermediaries connecting dietary intake to hepatobiliary cancers, highlighting microbiome-targeted dietary strategies as potential preventive interventions. Further research is required to confirm these causal mechanisms and facilitate the development of targeted prevention and therapeutic approaches.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"100"},"PeriodicalIF":3.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mastoparan-S from Sphodromantis viridis exhibits antimicrobial activity by disrupting bacterial membranes.","authors":"Jonggwan Park, Seulbi Lee, Da Dam Kang, Jun Hee Oh, Hyeongsun Kim, Yoonkyung Park","doi":"10.1186/s13568-025-01908-3","DOIUrl":"10.1186/s13568-025-01908-3","url":null,"abstract":"<p><p>The increasing prevalence of antimicrobial resistance underscores the urgent need for alternative treatments. Antimicrobial peptides (AMPs) are considered viable substitutes for conventional antibiotics due to their broad-spectrum activity and unique mechanisms of action. AMPs are an integral component of the innate immune system in invertebrates. Mastoparan-S, a 14-amino acid AMP originally identified in the praying mantis Sphodromantis viridis reportedly exhibits antimicrobial activity against gram-positive and gram-negative bacteria, as well as fungi. In this study, the antibacterial activity and mechanism of action of chemically-synthesized mastoparan-S are evaluated against Staphylococcus aureus and Pseudomonas aeruginosa. Mastoparan-S exhibits potent antibacterial activity against S. aureus and P. aeruginosa, without inducing cytotoxicity or hemolytic activity in RAW264.7 or HaCaT cells. Mastoparan-S effectively inhibits biofilm formation by S. aureus and P. aeruginosa. Mechanistically, mastopran-S kills bacteria by disrupting the bacterial membrane. Collectively, these findings suggest that mastoparan-S is a potential therapeutic agent for treating S. aureus and P. aeruginosa infections.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"99"},"PeriodicalIF":3.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examining miR-196a, miR-196b and NF-κBIα gene expression in colitis mice model.","authors":"Zahra Rafiezadeh, Shima Aboutalebian, Azar Baradaran, Laleh Hoveida, Seyed Hossein Hejazi","doi":"10.1186/s13568-025-01869-7","DOIUrl":"10.1186/s13568-025-01869-7","url":null,"abstract":"<p><p>Bifidobacterium bifidum (B. bifidum) anti-inflammatory characteristics and ability to modify the gut microbiota make it a promising treatment option for Inflammatory Bowel Disease (IBD). The present study investigated the modulatory effects of B. bifidum on the expression of miR-196a, miR-196b, and NF-κBIα genes in a DSS-induced IBD mice model. Thirty male C57BL/6 mice were randomly assigned to five experimental groups (G1-G5). The induction of colitis used 3% DSS in drinking water for 8 days. The Disease Activity Index (DAI) was monitored, and mice were euthanized for further analysis on the 22nd day. The mice were gavaged with 2 × 10⁹ CFU/ml of B. bifidum per day. Histological grading of the colon tissues was done, and the levels of cytokines TNF-α and IL-6 were measured using ELISA. At the gene level, the expression of miR-196a, miR-196b, and NF-κBIα was analyzed by RT-PCR. Weight gain from days 11 to 17 in G3 and G4 with B. bifidum treatment pointed out its therapeutic benefits. Colon length, weight, and spleen weight were significantly decreased in G2 compared to G1 (P ≥ 0.0001). Histopathological examination revealed severe mucosal damage in G2, reduced inflammation in G3, fewer deep wounds in G4, and significant healing in G5. B. bifidum, especially in G5, significantly reduced IL-6 and TNF-α levels (P ≥ 0.0002). Gene expression analysis showed decreases in miR-196a and NF-κBIα and an increase in miR-196b, with G5 showing the most changes (P ≥ 0.0001). In conclusion, B. bifidum demonstrated significant anti-inflammatory effects in the DSS-induced IBD model by modulating miRNA expression and reducing key inflammatory cytokines.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"98"},"PeriodicalIF":3.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota, plasma metabolites, and venous thromboembolism: a Mendelian randomization study.","authors":"Peng-Fei Cheng, Jin-Xi Wang, Ju-Shan Wu, Guang-Ming Li","doi":"10.1186/s13568-025-01903-8","DOIUrl":"10.1186/s13568-025-01903-8","url":null,"abstract":"<p><p>Emerging evidence links gut microbiota (GM) to venous thromboembolism (VTE), deep vein thrombosis (DVT), and pulmonary embolism (PE), but causal insights are limited. Using two-sample Mendelian randomization (MR), we assessed causal effects of 211 GM taxa and 489 plasma metabolites on VTE/DVT/PE. Genetic instruments (single nucleotide polymorphism) for GM and metabolites were leveraged to infer causality, complemented by mediation and pathway analyses. Sixteen GM taxa (e.g., protective Firmicutes, Clostridia; risk-enhancing Bacteroidetes, Desulfovibrionaceae) and 40 metabolites showed causal associations with VTE/DVT/PE. Reverse MR identified 11 GM changes secondary to thrombosis. No pleiotropy or heterogeneity was detected. Ten metabolite-mediated pathways (e.g., arginine biosynthesis) bridged GM to thrombosis, suggesting GM-metabolite interactions as potential biomarkers for thrombotic risk stratification and prognosis.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"97"},"PeriodicalIF":3.5,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus johnsonii HL79 mitigate plateau environment-induced hippocampal dysfunction in mice.","authors":"Baoxing Gan, Xufei Zhang, Jinge Xin, Lixiao Duan, Ning Sun, Yu Chen, Junqi Zeng, Yueying Lian, Hao Li, Hesong Wang, Xueqin Ni, Hailin Ma","doi":"10.1186/s13568-025-01898-2","DOIUrl":"10.1186/s13568-025-01898-2","url":null,"abstract":"<p><p>Plateau environment represents a common terrestrial characterized by multistress conditions including hypobaric hypoxia, low temperature, and intense radiation, yet sustain over 100 million permanent or transient inhabitants. While this extreme environment exerts profound impacts on cerebral architecture and gut microbiota homeostasis, precipitating cognitive deficits and microbiome-derived intestinal pathologies, the mechanistic interplay between plateau environment adaptation and microbial dynamics remains contentious. Here, we employ a microbiota-gut-brain axis framework to investigate whether probiotic intervention can ameliorate hippocampal impairments induced by simulated plateau environment exposure (3500-4000 m) in mice. Through simulated plateau environment exposure experiments, we revealed that extreme high-altitude conditions induced hippocampal memory dysfunction in mice, exacerbated oxidative stress damage in hippocampal tissues, and altered synaptic plasticity-related biomarkers including CREB transcription factor, BDNF protein levels, and electrophysiological power spectra. Administration of HL79 alleviated these burdens, including memory dysfunction and tissue damage, though complete reversal was not achieved. Combined hippocampal transcriptomic analyses suggested that HL79's beneficial effects primarily involved modulation of lipid-related gene expression in the hippocampus, consistent with prior reports of plateau environmental impacts on gene expression. Serum metabolomic results further reinforced this inference that differential metabolites regulated by HL79 are mainly enriched in bile secretion, taurine and hypotaurine metabolism, linoleic acid metabolism, and PPAR signaling pathways, though the precise regulatory mechanisms require further elucidation. This research provides a novel microbiota-gut-brain axis-based regulatory strategy for adaptation to extreme plateau environments and offers new evidence for understanding the relationship between gut microbiota and plateau environment adaptation at high elevations.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"96"},"PeriodicalIF":3.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12179028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}