AMB Express最新文献

{"title":"Correction to: Production of highly cytotoxic and low immunogenic L-asparaginase from Stenotrophomonas maltophilia EMCC2297.","authors":"Nada A Abdelrazek, Sarra E Saleh, Marwa M Raafat, Amal E Ali, Mohammad M Aboulwafa","doi":"10.1186/s13568-025-01848-y","DOIUrl":"10.1186/s13568-025-01848-y","url":null,"abstract":"","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"57"},"PeriodicalIF":3.5,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional characterization of glycosyltransferase-related biomarkers for tuberculosis diagnosis.","authors":"Yibiao Zhang, Xiaoyun Ye, Jiancong Xu, Jixiao He, Xiaobing Lu","doi":"10.1186/s13568-025-01870-0","DOIUrl":"10.1186/s13568-025-01870-0","url":null,"abstract":"<p><p>Tuberculosis (TB) is an infectious disease that presents a serious risk to public health. Glycosyltransferase-related genes (GTRGs) are instrumental in assessing the risk of latent tuberculosis infection progressing to active TB. This study aims to develop novel, accurate, and effective diagnostic markers to enhance the early diagnosis and precision treatment of TB. We employed Weighted Gene Co-expression Network Analysis (WGCNA) to explore key genes that are notably linked toTB. In addition, we employed single-sample Gene Set Enrichment Analysis (ssGSEA) to examine the differences in immune cell infiltration between normal tissues and those affected by TB. The effectiveness of the potential biomarkers was evaluated through Receiver Operating Characteristic (ROC) curves and their expression patterns. We also conducted single-gene enrichment analysis to explore the biological functions and pathway activities linked to the characteristic genes. Finally, we constructed a competitive endogenous RNA (ceRNA) network to elucidate the potential regulatory mechanisms governing these genes. Through the screening of hub genes and differentially expressed genes from the GTRGs, we identified two potential biomarkers: B4GALT5 and KCNJ2. Evaluation results indicated that these characteristic genes displayed strong diagnostic performance in both the training and validation cohorts. Moreover, single-gene enrichment analysis revealed that these genes were primarily enriched in apoptosis pathways closely associated with TB treatment. Additionally, the construction of the mRNA-miRNA-lncRNA network identified 82 miRNAs and 65 lncRNAs. This study elucidates the roles of GTRGs in TB, identifies biomarkers associated with these groups, and establishes the lncRNA expression profile of characteristic genes. These findings provide a theoretical foundation for the early diagnosis of TB.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"56"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-virulence effect of photoactivated nano-quercetin by diode laser on Aggregatibacter actinomycetemcomitans.","authors":"Sara Monem Moharrer, Maryam Pourhajibagher, Arash Azizi, Arezoo Alaee","doi":"10.1186/s13568-025-01868-8","DOIUrl":"10.1186/s13568-025-01868-8","url":null,"abstract":"<p><p>Photodynamic therapy (PDT) is an innovative, non-invasive, and selective treatment approach that has gained attention as an adjunctive treatment in periodontitis. Aggregatibacter actinomycetemcomitans is an opportunistic periodontopathogen known for its ability to form biofilms. The objective of this in vitro study was to determine the anti-virulence effect of nano-quercetin (N-QT)-mediated PDT on the expression of a gene associated with biofilm formation of A. actinomycetemcomitans. After synthesis and characterization of N-QT by transmission electron microscopes (TEM), dynamic light scattering (DLS), zeta potential analysis, and fourier transform infrared spectroscopy (FTIR) analysis, the effects of different concentrations of N-QT and energy densities of diode laser alone on cell viability of A. actinomycetemcomitans were evaluated. A. actinomycetemcomitans was then treated by sub-lethal doses of diode laser emitting light in the presence of sub-lethal doses of N-QT. Ultimately, the sub-lethal dose of PDT was used to investigate the metabolic activity of A. actinomycetemcomitans, the generation of reactive oxygen species (ROS), and changes in rcpA, qseB, and qseC genes expression using the quantitative real-time polymerase chain reaction (qRT-PCR) method. According to the results, TEM, DLS, zeta potential, and FTIR confirmed the synthesis of N-QT. PDT with concentrations of 16 to 512 µg/mL of N-QT alone and irradiation for 6 min with an energy density of 154.8 J/cm² alone caused a significant decrease in bacterial count (P < 0.05). PDT with 8 µg/mL N-QT and 5-min irradiation achieved the highest metabolic activity reduction (42.9%), while 2 µg/mL N-QT and 4-min irradiation yielded the lowest reduction (12.3%). Using 8 µg/mL of N-QT with light exposure for 4 and 5 min at energy densities of 103.9 and 129 J/cm² led to ROS levels that were 2.67- and 2.83-fold higher than those in the control cells, respectively (P < 0.05). Additionally, the sub-lethal dose of PDT with 5 min of irradiation in the presence of 2 µg/mL of N-QT resulted in a 2.62-, 2.85-, and 3.21-fold decrease in the expression levels of the rcpA, qseB, and qseC genes, respectively (P < 0.05). PDT utilizing N-QT and diode laser shows promise in suppressing cellular survival and emerges as a viable strategy for targeting A. actinomycetemcomitans virulence.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"55"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel Enterococcus durans with antimicrobial, anti-diabetes and anti-alzheimer activities isolated from Egypt.","authors":"Hussam H Arafat, Mahmoud A Shoulkamy, Mohamed M Imam, Amany M A Ali","doi":"10.1186/s13568-025-01836-2","DOIUrl":"10.1186/s13568-025-01836-2","url":null,"abstract":"<p><p>Enterococcus sp. is a subset of lactic acid bacteria that have beneficial effects on human health including prevention of gut chronic diseases, controlling Alzheimer and diabetes. The study involves morphological and biochemical identification of Enterococcus sp. and 16S rRNA gene sequencing methods, alongside an exploration of it's antimicrobial, anti-diabetes and anti-Alzheimer efficacy. The cell-free supernatant (CFS) derived from this isolate (Enterococcus durans) exhibited a significant antibacterial activity against both Gram-positive (Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis) with inhibition zones of 31.1, 33 and 27 mm, respectively and Gram-negative (Escherichia coli, Klebsiella pneumoniae, Salmonella typhi) bacteria, recording inhibition zones of 24, 25 and 30 mm, respectively. The least values of MIC and MBC were 15.62 µg/ml and 31.25 µg/ml, respectively; in case of Bacillus subtilis and Staphylococcus aureus. The highest MIC and MBC were 62.5 µg/ml and 250 µg/ml respectively, when testing CFS against Klebsiella pneumonia. Notably, the stability of CFS was maintained at various temperatures, including autoclaving conditions (121 ℃). The isolate displayed tolerance across a wide pH range (2.5-9.5), with enhanced activity observed at acidic pH levels. Butyrylcholinesterase inhibition was estimated to be 84.6%, while amylase inhibition was 97.6% & 94.2%, respectively. GC-MS revealed metabolites not defined previously in enterococci: 1H-purin-6-amine, [(2-fluorophenyl) methyl]-(29.72%), hexadecanoic acid, 2, 3 dihydroxypro polyester (18.60%), oleic acid (11.60%) and 9-octadecenamide (6.54%). Hence, our strain is a reservoir of strong bioactive compounds, with antimicrobial, anti-diabetes and anti- Alzheimer potentials.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"54"},"PeriodicalIF":3.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11947379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"His⁷⁰ of Acetivibrio alkalicellulosi Cel5A is important for efficient hydrolysis of short cellodextrins.","authors":"Mengxiang Jia, Yangyang Chen, Jingting Wang, Jiahan Wang, Yihua Ma, Yujiao Wang, Qian Ma, Yiheng Zhang, Weidong Liu, Kuanqing Liu","doi":"10.1186/s13568-025-01858-w","DOIUrl":"10.1186/s13568-025-01858-w","url":null,"abstract":"<p><p>Cellulose, a linear glucan linked by β-1,4 glycosidic bonds, is the most abundant renewable polysaccharide on earth. Complete enzymatic hydrolysis of cellulose liberates the readily metabolizable glucose that could be further converted to valuable biocommodities, and essential to this process are cellulases that hydrolyze the β-1,4 glycosidic bonds. Cellulases are among the most intensively studied and best understood enzymes, and many key residues have been uncovered and interrogated with respect to their functions in catalysis and/or substrate binding. However, it remains to be explored whether additional residues, especially in many poorly characterized cellulases such as processive endoglucanases, might also be functionally important. Here, we investigated a processive endoglucanase from an alkaliphilic bacterium Acetivibrio alkalicellulosi AaCel5A that consists of a glycohydrolase family 5 (GH5) domain and two tandem carbohydrate-binding module family 6 (CBM6) domains. Via structure-guided engineering, we uncovered the functional importance of a previously underexplored but relatively conserved histidine (histidine70 or His⁷⁰). His⁷⁰ itself appears to be largely dispensable for hydrolyzing β-1,4 glycosidic bonds, but it is important for efficient hydrolysis of short cellodextrins such as cellotriose, cellotetraose, and cellopentaose, likely through its ability to coordinate substrate binding. Our work thus provides important mechanistic insights into how processive endoglucanases may act on short cellodextrins.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"53"},"PeriodicalIF":3.5,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfo-phospho-vanillin method for screening Aurantiochytrium strains with high docosahexaenoic acid levels.","authors":"Person Pesona Renta, Ta-Yu Huang, Ping-Hao Yu, Anna C-C Jang, Yi-Min Chen","doi":"10.1186/s13568-025-01859-9","DOIUrl":"10.1186/s13568-025-01859-9","url":null,"abstract":"<p><p>Aurantiochytrium are protists found in marine and estuarine environments, known for producing high quantities of omega-3 fatty acids, particularly docosahexaenoic acid (DHA). The commercial viability of this species is currently hindered by the lack of reliable screening methods for the rapid identification of strains with high DHA content. This study developed a high-throughput screening platform based on the sulfo-phospho-vanillin (SPV) reaction, which produces a pink chromophore upon reacting with C-C double bonds in lipids. Analysis of 200 strains derived through the UV mutagenesis of the Aurantiochytrium limacinum strain BL10 revealed 7 strains that exhibited significantly elevated SPV reactivity, compared to the naïve strain (P < 0.01). Gas chromatography-mass spectrometry analysis revealed that in 4 of the 7 strains, DHA levels were significantly higher than those of the naïve strain. Among those 4 strains, the SPV reactivity values of two strains were precisely correlated with their DHA/DPA content. The SPV reaction protocol proposed in this paper has considerable potential for the high-throughput screening of Aurantiochytrium strains particularly those derived through UV mutagenesis.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"52"},"PeriodicalIF":3.5,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction of grape seeds by different solvents affects the activities of the resultant extract.","authors":"Kareem Tarek, Alyaa Farid, Gehan Safwat","doi":"10.1186/s13568-025-01851-3","DOIUrl":"10.1186/s13568-025-01851-3","url":null,"abstract":"<p><p>Phenolic compounds are concentrated in grape seeds; 60-70% of the extractable grape phenols are found in the seeds. The focus of this research was to isolate the phytochemicals from grape seed and to determine their ability to prevent haemolysis, their antioxidant and microbiological activities. By using the extraction procedure, three solvents were used (distilled water, ethanol and methanol). A high-performance liquid chromatographic (HPLC) test was performed to analyse the phenolic compounds and flavonoids content that were used to determine the efficiency of the various solvents used in the extraction process. All the variables under study, namely yield percentage, phenolic component concentration, and flavonoid content got significantly affected by the choice of the solvent used. The flavonoid content of the extracts was in the order methanolic extract > ethanolic extract > water extract. The methanolic extract of the grape seeds exhibited the most powerful antioxidant and hemolysis inhibitory effects among the three extracts, followed by the ethanolic and water extracts. The antibacterial activity of methanolic extract was found to be higher as compared to the ethanolic extract against Staphylococcus aureus. The antibacterial activity of the ethanolic and methanolic extracts against Salmonella enteritidis, Bacillus subtilis, Aspergillus niger and Escherichia coli were found to be equivalent. In conclusion, grape seeds contained several bioactive compounds that exerted an antioxidant, hemolysis inhibition and anti-microbial activities. These activities depends on the concentration of phenolic compounds and flavonoids in the grape seed extracts. Methanol was the superior solvent in the extraction process followed by ethanol.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"51"},"PeriodicalIF":3.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis and metabolic engineering of natural sweeteners.","authors":"Bengui Fan, Xiqin Liang, Yichi Li, Mingkai Li, Tongle Yu, Yuan Qin, Bohan Li, Tianyue An, Guoli Wang","doi":"10.1186/s13568-025-01864-y","DOIUrl":"10.1186/s13568-025-01864-y","url":null,"abstract":"<p><p>Natural sweeteners have attracted widespread attention because they are eco-friendly, healthy, low in calories, and tasty. The demand for natural sweeteners is increasing together with the popularity of green, low-carbon, sustainable development. With the development of synthetic biology, microbial cell factories have emerged as an effective method to produce large amounts of natural sweeteners. This technology has significantly progressed in recent years. This review summarizes the pathways and the enzymes related to the biosynthesis of natural sweeteners, such as mogrosides, steviol glycosides, glycyrrhizin, glycyrrhetinic acid, phlorizin, trilobatin, erythritol, sorbitol, mannitol, thaumatin, monellin, and brazzein. Moreover, it focuses on the research about the microbial production of these natural sweeteners using synthetic biology methods, aiming to provide a reference for future research on the production of natural sweeteners.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"50"},"PeriodicalIF":3.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of Staphylococcus aureus-infected diabetic wounds by melatonin loaded nanocarriers.","authors":"Alyaa Farid, Ayah Mohsen, Bassant Nasser, Habiba Alaa, Mariam Abdelaziz, Maryam Mustafa, Mustafa Mansour, Nourhan Adel, Salma Magdy, Salma Mohsen, Samah Adel, Sarah Ibrahim, Shaymaa Abdel-Rahman, Sohaila Mohamed, Yomna El-Karamany","doi":"10.1186/s13568-025-01854-0","DOIUrl":"10.1186/s13568-025-01854-0","url":null,"abstract":"<p><p>One of the complication of diabetes mellitus is chronic wounds. The healing of wounds in diabetic patients is retarded by the elevation in the pro-inflammatory cytokines secretion and free radicles accumulation. Wound management in diabetic patients requires preventing bacterial biofilm development. Due to the wound healing activity of chitosan (CS), lecithin (Le) and melatonin (M), the present study aimed to load melatonin on CS/Le NPs and examine their effect on diabetic wounds infected with Staphylococcus aureus. Melatonin loaded chitosan/lecithin nanoparticles (M-CS/Le NPs) were physically characterized and their antioxidant, anti-inflammatory and antimicrobial activities were examined in vitro. Male Sprague Dawley rats included two division (non-diabetic and diabetic) which were further divided in nine groups. Diabetes induction and follow up throughout the experimental period was confirmed by measuring the levels of fructosamine and blood glucose. Full-thickness wounds was induced in both non-diabetic and diabetic animals followed by infection with Staphylococcus aureus according to the experimental design. The wound healing effect of M-CS/Le NPs was evaluated through measurements of the oxidative stress, inflammatory cytokines and apoptotic proteins. Our results showed the anti-microbial, free radical scavenging and hemolysis inhibition effects of M-CS/Le NPs in vitro. Moreover, the preparation of M-CS/Le NPs decreased the dose of used melatonin (when compared to free melatonin). M-CS/Le NPs significantly decreased the wound area percent in treated infected wounds of both non-diabetic and diabetic rats more than free melatonin or unloaded CS/Le NPs. In conclusion, M-CS/Le NPs promoted the wound healing in Staphylococcus aureus-infected wounds in diabetic rats.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"46"},"PeriodicalIF":3.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of African swine fever virus by a blue latex microsphere immunochromatographic strip.","authors":"Yanli Hu, Yingjun Xia, Huiwen Mo, Li Zhang, Wenjian Cao, Rui Fang, Junlong Zhao","doi":"10.1186/s13568-025-01857-x","DOIUrl":"10.1186/s13568-025-01857-x","url":null,"abstract":"<p><p>African swine fever (ASF) is a contagious viral disease in pigs, caused by the African swine fever virus (ASFV). Currently, there are no effective vaccines and drugs for ASFV, making diagnostic methods crucial for the prevention and control of ASF. In this study, the ASFV p30 protein was successfully expressed using a prokaryotic expression system and used as an immunogen to prepare monoclonal antibody (mAb) 2A5. The antigenic epitope recognized by the mAb 2A5 was identified as ⁵⁸VKYDIVKSARIYAGQGY⁷⁴. Then, a blue latex microsphere immunochromatographic strip method for detecting ASFV antigens was established. Wherein a rabbit polyclonal antibody (pAb) against p30 stored in our laboratory was coupled with blue latex microspheres to prepare an immune probe, with mAb 2A5 as the test line and goat anti-rabbit IgG as the control line. The immunochromatographic strip exhibited excellent sensitivity, with a minimum detection limit of 10 ng/mL for the p30 protein. It demonstrated good specificity, showing no cross-reactivity with PRRSV, PCV2, PPV, and PEDV. Moreover, it maintained good stability and could be stored for at least 100 days at 37 °C. The concordance rate between the immunochromatographic strip and qPCR test kit was 92.2%. The immunochromatographic strip method can meet the requirements of clinical testing and provide technical support for ASFV diagnosis.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"15 1","pages":"48"},"PeriodicalIF":3.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}