AMB Express最新文献

{"title":"Steering the microbial ecosystem: how ethanol boosts coalbed methane output.","authors":"Yingying Li, Yu Meng, Huairen Zhang, Mingqiang Qiao, Xiuqing Yang","doi":"10.1186/s13568-026-02047-z","DOIUrl":"10.1186/s13568-026-02047-z","url":null,"abstract":"<p><p>Microbial-enhanced coalbed methane (MECBM) technology is considered a key approach to increasing CBM yields. However, the biogenic conversion of coal to methane is limited by coal’s recalcitrant macromolecular structure, which constrains production. Therefore, enhancing microbial degradation efficiency is critical for improving CBM yields. To elucidate the mechanism through which ethanol enhances coal biodegradation and methanogenesis, this study employed an integrated approach combining 13C-stable isotope tracing with multi-omics analyses. This strategy enabled investigation of the bioconversion dynamics related to methane, volatile fatty acids (VFAs), and the ethanol-responsive microbial community. The addition of ethanol (2 mg/mL) markedly enhanced methane and hydrogen production by 10.9-fold and 3.1-fold, respectively. It also significantly elevated the soluble chemical oxygen demand (SCOD) level. Ethanol selectively stimulated the proliferation of key hydrogen-producing acetogens, including Clostridium, Syntrophomonas, and Petrimonas, while concurrently enhancing the cooperative interactions between these bacterial populations and methanogenic archaea. Isotope tracking confirmed that the direct incorporation of ethanol-derived carbon into methane, carbon dioxide, and VFAs. Furthermore, the abundance of key functional genes involved in ethanol oxidation, acetic acid metabolism, and central carbon pathways was enriched. This genetic response correlated with increased organic acids production and enhanced mobilization of soluble compounds derived from coal. Collectively, these findings offer novel insights into the stimulatory effects of ethanol on coal bioconversion and highlight a promising strategy to improve coalbed methane (CBM) recovery and utilization.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13194848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asiatic acid disrupts biofilm formation and shows additive effect with antibiotic against Acinetobacter baumannii.","authors":"Anh Ngoc Xuan Nguyen, Made Rai Dwitya Wiradiputra, Krit Thirapanmethee, Teerawit Audshasai, Pimonrat Ketsawatsomkron, Mullika Traidej Chomnawang","doi":"10.1186/s13568-026-02052-2","DOIUrl":"10.1186/s13568-026-02052-2","url":null,"abstract":"<p><p>Asiatic acid, a bioactive pentacyclic triterpenoid derived from Centella asiatica, has been widely used against infectious wounds and inflammation. Recently, this natural compound has also drawn attention to interfering with bacterial biofilms, a major phenotypic strategy that enables bacteria to survive under stress or antibiotic pressure. Acinetobacter baumannii, a nosocomial pathogen, is well known for its remarkable ability to form biofilms that promote chronic infections and contribute to conventional antibiotic resistance. The present study investigated the inhibitory effect of asiatic acid during the early stages of A. baumannii biofilm development. Despite exhibiting indirect antimicrobial activity, asiatic acid significantly reduced biofilm production in both drug-resistant clinical isolates and A. baumannii ATCC 19606 reference strain. Confocal microscopic evaluations supported the visual attenuation of biofilm structure following treatment. Molecular analyses revealed that asiatic acid downregulated the expression of critical biofilm-associated adhesion genes, csuE and bap. By suppressing these genes, this natural compound may prevent bacteria from surface attachment and interfere with the subsequent maturation of biofilms. Furthermore, asiatic acid additively contributed to antimicrobial activity of standard antibiotics against A. baumannii. These findings support the potential of asiatic acid as an adjunctive agent for the control of biofilm-associated infections caused by A. baumannii, particularly in the context of rising multidrug resistance. Continued investigation into its mechanisms and synergistic combinations may inform the development of novel anti-biofilm therapeutic strategies.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13194852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eicosadienoic acid enhances antibody production in CHO cells by prolonging culture longevity and attenuating apoptosis.","authors":"Xinxin Pei, Xiaoyi Liu, Rui Wang, Tengfei Ma, Buchang Zhang, Changzhi Xu","doi":"10.1186/s13568-026-02050-4","DOIUrl":"10.1186/s13568-026-02050-4","url":null,"abstract":"<p><p>Fatty acids are key nutrients exerting multiple functions in cells. This study aimed to investigate the effect of fatty acids on antibody expression in Chinese hamster ovary (CHO) cells. Among different fatty acids, cis, cis − 11, 14-eicosadienoic acid (EDA, C20:2, Δ11,14-cis) was screened to exert the strongest effect to boost antibody yield in CHO-IgG cells tested. In batch and fed-batch culture processes, the antibody yield was promoted by 48% and 59% with supplementation of 10 µM of EDA respectively. Meanwhile, the culture duration was significantly prolonged while the apoptosis ratios were suppressed by EDA. The addition of EDA also promoted the yield of other antibodies transiently expressed in CHO-K1 cells, including Rituximab, Herceptin, and one anti-uPAR antibody. These findings revealed the potential of EDA in promoting antibody expression in CHO cells, shedding light on the application of functional fatty acids in therapeutic antibody manufacturing.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13194837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brewing water affects microbial community and aromatic profiles during Shaoxing rice wine fermentation.","authors":"Mengwei Zhang, Yuelei Ruan, Songtao Ge, Ying Ling, Xiaowei Han, Rongshe Zhang","doi":"10.1186/s13568-026-02051-3","DOIUrl":"10.1186/s13568-026-02051-3","url":null,"abstract":"<p><p>Shaoxing rice wine (Huangjiu) is characterized by its unique quality and flavor, however, the impact of different brewing water on the microbial community during fermentation and aromatic characteristics of Huangjiu product is less elucidated. In this study, water from Jianhu Lake and Jingling Reservoir in Shaoxing were used for brewing Huangjiu, and the purified water was used as the control. The fermentation mash at different fermentation stages was collected and sequenced to investigate the alterations of bacterial and fungal communities. Besides, the final fermented Huangjiu was analyzed for its volatile compound profiles using Gas Chromatography–Mass Spectrometry (HS-SPME/GC-MS). Water from Jingling Reservoir had higher levels of total nitrogen, chemical oxygen demand, iron and manganese ions, but lower levels of hardness and total viable counts than that from Jianhu Lake. The bacterial and fungal communities during Huangjiu fermentation were significantly different using different brewing water, as assessed by principal coordinates analysis. Besides, bacterial genera, including Enterobacter, Staphylococcus, Pantoea, Leuconostoc, Acinetobacter, Exiguobacterium, Janthinobacterium, and Limnohabitans, were significantly different. The co-occurrence network analysis identified the core genera in bacterial community, including Lactococcus, Leuconostoc, Exiguobacterium, and Pediococcus. HS-SPME/GC-MS results demonstrated 19 volatile compounds were significantly different using different brewing water. The correlation analysis further revealed the key microbial genera which are tightly associated with the alteration in aromatic characteristics using different brewing water, including 16 bacterial and 2 fungal genera. Collectively, our results demonstrated the alterations of microbial communities and aromatic profile of Huangjiu using different brewing water, and provided experimental basis for understanding the significance of brewing water in Huangjiu’s flavor.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13168412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced secretion of an Agrocybe aegerita peroxygenase variant in K. phaffii using the native signal peptide.","authors":"Ginevra Camboni, Rebecca Preece, Katy A S Cornish, Jared Cartwright, Gideon Grogan","doi":"10.1186/s13568-026-02049-x","DOIUrl":"10.1186/s13568-026-02049-x","url":null,"abstract":"<p><p>Unspecific Peroxygenases (UPOs) are enzymes with significant potential as catalysts in synthetic chemistry as they catalyse selective oxygenation reactions on organic substrates at the expense of only hydrogen peroxide as the external oxidant. The demand for UPOs has stimulated considerable research into efficient heterologous expression systems for their production, which have included optimisation of the signal peptide (SP) included upstream of the UPO gene. In this study we report a comparison of the production of the prototypical and widely-used UPO variant from the fungus Agrocybe aegerita (rAaeUPO-PaDa-I-H) using both its native SP and variant SPs evolved for improved heterologous expression in yeast hosts. The results show that improvements in protein production identified using variant SPs in S. cerevisiae are not necessarily extended to the yeast Komagataella phaffii. Indeed in K. phaffii we observed a five to sevenfold improvement in the activity of crude secretates produced using the native fungal SP of AaeUPO, compared with SPs that were evolved for improved expression and screened in S. cerevisiae These findings suggest that superior yields of this widely-used UPO can be obtained from scaled production in K. phaffii by using the native SP from the source fungus.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13168419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics analysis reveals the response mechanism of Pichia kudriavzevii to high-concentration lactic acid stress.","authors":"Weiwei Li, Ning Wang, Jia Zheng, Ahmed H El-Sappah, Huibo Luo, Weiming Jiang, Rongfei Wang, Linjuan Li, Puyu Zhang, Dalong Liao, Hong Wang, Hui Zhu, Muhammad A Mehmood","doi":"10.1186/s13568-026-02046-0","DOIUrl":"10.1186/s13568-026-02046-0","url":null,"abstract":"<p><p>Pichia kudriavzevii tolerance mechanisms are not well understood, even though strong-flavor Baijiu fermentation places a heavy burden on yeast metabolism due to the accumulation of lactic acid (LA). This study utilized growth kinetics, electron mi-croscopy, antioxidant tests, and integrated metabolomic-transcriptomic studies to an-alyze strain Y2-1, which was isolated from Baijiu pit yellow water. Y2-1 showed a de-layed biphasic growth pattern and could withstand up to 80 g/L of LA. It appears that morphological adaptation plays a role in stress resistance, as scanning electron mi-croscopy showed that cells elongated significantly at high LA levels. Enrichment in pyruvate metabolism, glutathione metabolism, and ABC transporter pathways was among the 1,125 differentially expressed genes and 817 different metabolites found using multi-omics analysis. In order to maintain membrane integrity, ergosterol homeo-stasis, and mitochondrial function, mechanistically speaking, LA tolerance entailed three strategies: (i) activating pyruvate metabolism, which channeled lactate into energy metabolism and mitigated proton imbalance; (ii) enhancing the glutathione antioxidant system, which included spermidine synthase upregulation and improved ROS scavenging and intracellular pH buffering; and (iii) induction of ABC transporters (SNQ2, ABCB7). Molecular insights into yeast acid tolerance and a foundation for creating robust strains to boost Baijiu fermentation efficiency are offered by these findings, which indicate a metabolic-transport-linked defense strategy.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13153303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147571833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of growth stage and moisture content on the fermentation profile, chemical composition, and bacterial community structure of triticale silage.","authors":"Mingze Lan, Maoya Li, Yao Lei, Qiming Cheng, Jiachuhan Wang, Yuanyuan Zhao, Xiangjiang He, Guoxiong Hu, Chao Chen","doi":"10.1186/s13568-026-02045-1","DOIUrl":"10.1186/s13568-026-02045-1","url":null,"abstract":"<p><p>Triticale (× Triticosecale wittmack) is a high-yielding and nutritionally balanced forage crop with considerable potential for silage production. This study examined the effects of growth stage and moisture content on the fermentation quality and bacterial community composition of triticale silage by harvesting at heading and dough stages and ensiling at 60%, 70%, and 80% moisture content for 60 days. Results showed that both factors significantly affected nutritional components, pH, lactic acid, acetic acid, and ammonia nitrogen concentrations; silage from the dough stage generally had higher lactic acid levels and lower pH and ammonia nitrogen, indicating more favorable fermentation than that from the heading stage. Ensiling at 70% moisture resulted in higher crude protein and water-soluble carbohydrate contents, lower neutral and acid detergent fiber concentrations, greater lactic acid production, and lower pH, corresponding to improved fermentation characteristics. Bacterial community analysis revealed that 70% moisture enhanced the relative abundances of Firmicutes and Lactiplantibacillus plantarum and reduced Proteobacteria and other undesirable microorganisms. In summary, harvesting triticale at the dough stage with a controlled moisture content of 70% contributes to improved fermentation quality and a beneficial microbial community, providing a theoretical basis and technical support for high-quality triticale silage production in sustainable livestock agriculture.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13144472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147525256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phage-antibiotic combination: a possible approach to combatting multidrug-resistant Klebsiella pneumoniae.","authors":"Nourhan I Abdelhakim, Walaa A Alshareef, Sarra E Saleh, Mahmoud A Yassein","doi":"10.1186/s13568-025-02005-1","DOIUrl":"10.1186/s13568-025-02005-1","url":null,"abstract":"<p><p>Multidrug-resistant Klebsiella pneumoniae is a major global health concern due to its role in severe hospital-acquired infections and resistance to multiple antibiotics. As conventional antibiotics lose effectiveness, bacteriophage (phage) therapy offers a promising alternative. This study assessed phage therapy alone and combined with antibiotics against multidrug-resistant K. pneumoniae using the time-kill assay. A total of 48 clinical isolates were collected and identified as K. pneumoniae. According to their antibiotic susceptibility, 91.7% (n = 44) of the tested isolates were multidrug-resistant. Sewage samples were processed to isolate a phage-coded Kp25, which showed lytic activity against 17 isolates and remained stable within a temperature range of 40–90 °C and a pH range of 3–11. The taxonomic classification of the phage was made clear by the findings of the genomic analysis, which is Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Drexlerviridae; Webervirus; Webervirus domnhall. Phage combination with sub-MIC of levofloxacin and meropenem showed potential antibacterial activity, so that complete in vitro bacterial eradication was achieved starting from 4 h incubation until the end of the incubation period. However, phage combination with sub-MIC of gentamicin showed weak antibacterial activity. These findings highlight the phage combination with levofloxacin and meropenem as an effective strategy against multidrug-resistant K. pneumoniae.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13065991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Process optimization of compound fermented traditional Chinese medicine and its feeding effect on dairy cows.","authors":"Man Zhang, Zhiyi Zhao, Zhewei Zhang, Yanting Sun, Yu Kang, Hui Ma, Yuchang Ning, Xiaozhan Zhang, Fayin Tang, Zhanyong Wei, Chuanzhou Bian, Hongxing Qiao","doi":"10.1186/s13568-026-02038-0","DOIUrl":"10.1186/s13568-026-02038-0","url":null,"abstract":"<p><p>This study evaluated the efficacy of a compound fermented traditional Chinese medicine (TCM) preparation in dairy cows. Five TCMs and three probiotics were selected from 25 candidate herbs and six probiotics based on in vitro antibacterial activity against Staphylococcus aureus and Escherichia coli using agar well diffusion and paper disc diffusion methods. The five TCMs were formulated according to the \"Jun-Chen-Zuo-Shi\" principle, and the optimal solid-state fermentation process was identified through single-factor and orthogonal experiments. The results showed that the optimal conditions included a Bacillus subtilis: Bacillus coagulans: Lactobacillus plantarum ratio of 1:3:1, a moisture content of 32%, an inoculation rate of 10%, a temperature of 31 °C, and a fermentation duration of 5 d. HPLC was used to examine changes in bioactive compound levels before and after fermentation. Fermentation significantly increased the concentrations of Resveratrol, Salvianolic acid B, and Quercetin, whereas the Polydatin content decreased. To determine in vivo effects, the fermented TCM was fed to dairy cows, and the indices of production performance, milk quality, serum biochemical levels, immune function, antioxidant capacity, and hormone levels were evaluated. Additionally, the changes in intestinal flora were analyzed by sequencing rectal contents using 16 S rRNA. The results showed that fermented TCM could improve the production performance of dairy cows and milk quality, enhance humoral immunity, strengthen antioxidant capacity, induce modest shifts in gut microbiota composition, and regulate metabolism. This study provides reliable experimental evidence supporting the development and application of compound fermented TCM as a functional feed additive in dairy production.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13287534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics and mendelian randomization uncover microbial-metabolite-gene interactions in age-related hearing loss.","authors":"Sisi Li, Chunchun Zhou, Qiongmin Zhang","doi":"10.1186/s13568-026-02040-6","DOIUrl":"10.1186/s13568-026-02040-6","url":null,"abstract":"<p><p>Age-related hearing loss (ARHL), as one of the most common sensory impairments among the elderly, has a complex etiology and lacks effective targeted intervention strategies. Through integrated multiomics analysis, we identified 38 ARHL core-associated candidate genes that are predominantly enriched in pathways such as NF-κB signaling, antigen presentation, and oxidative phosphorylation. These findings suggest potential metabolic dysregulation and features consistent with \"inflammatory aging\" in the cochlea. Mendelian randomization analysis further highlighted UROS, C1QB, and GLIPR1 as potential biomarkers with high predictive value (AUC 0.948-0.970) within our dataset. Analysis of immune infiltration revealed a positive association between GLIPR1 and Th2 and neutrophil expression. Through correlation analysis and GutMgene database screening, we observed that FPR2 and GLIPR1 are significantly co-expressed. Molecular docking and molecular dynamics simulations further suggested that FPR2 can bind to taurodeoxycholic acid with high stability. Based on these findings, we hypothesize the existence of a \"Roseburia-FPR2-taurodeoxycholic acid\" regulatory network that may contribute to ARHL. This speculative model provides a novel framework for future investigations into the potential role of the gut-inner ear axis in ARHL.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13287190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147490565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}