Agricultural Sciences in China最新文献

{"title":"Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis","authors":"SUN Dong-bo,&nbsp;WU Rui,&nbsp;HE Xian-jing,&nbsp;WANG Shuang,&nbsp;LIN Yun-cheng,&nbsp;HAN Xu,&nbsp;WANG Yue-qiang,&nbsp;GUO Ting-ting,&nbsp;WU Guo-jun,&nbsp;YANG Ke-li","doi":"10.1016/S1671-2927(11)60160-0","DOIUrl":"10.1016/S1671-2927(11)60160-0","url":null,"abstract":"<div><h3>Abstract</h3><p><em>Staphylococcus aureus, Escherichia coli</em> and <em>Bacillus cereus</em> are the major agents of cow endometritis in dairy cows. A multiplex PCR (SEB-mPCR) was established based on the conserved genes of <em>S. aureus, E. coli</em> and <em>B. cereus,</em> and the detection limits were 10³, 10² and 10³ CFU mL-¹, respectively. SEB-mPCR could not amplify genomic DNA of pathogenic bacteria of other common bovine diseases. A total of 309 vaginal discharge samples from cows with endometritis were tested by SEB-mPCR. Of the samples, 23.95% had the three kinds of bacteria detected, 17.15% had <em>S. aureu</em> andii. <em>coli,</em> 9.39% had <em>E. coli</em> and <em>B. cereus,</em> and 9.71% had <em>S. aureus</em> and <em>B. cereus.</em> The rates of infections with <em>S. aureus, E. coli</em> and <em>B. cereuswere</em> 11.35, 16.18 and 9.06%, respectively. Therefore, SEB-mPCR has a potential as a diagnosis tool for endometritis in dairy cows.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 10","pages":"Pages 1624-1629"},"PeriodicalIF":0.0,"publicationDate":"2011-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60160-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Raising on Water Stocking Density Reduces Geese Reproductive Performances via Water Bacteria and Lipopolysaccharide Contaminations in “Geese-Fish” Production System","authors":"Dan-li JIANG ,&nbsp;Li LIU ,&nbsp;Cong-li WANG ,&nbsp;Fang CHEN ,&nbsp;Ai-dong SUN ,&nbsp;Zhen-dan SHI","doi":"10.1016/S1671-2927(11)60139-9","DOIUrl":"10.1016/S1671-2927(11)60139-9","url":null,"abstract":"<div><p>This study was carried out to unravel the mechanism of reductions in production performances in high stocking density geese flocks during summer months in “geese-fish” production system. Experiment 1 observed the water bacterial growth, lipopolysaccharde concentrations in water and geese blood, and geese reproductive performances from summer to winter, in two flocks with varying on water stocking densities. Results showed that counts of total bacteria, <em>Escherichia coli</em> and <em>Salmonella</em> in water, as well as water and geese plasma LPS concentrations, exhibited a tendency decreasing from the highest levels in summer, to intermediate levels in autumn, and to the lowest values in winter. Such seasonal decreases in bacteria and LPS concentrations were associated with similar seasonal decreases in embryo mortality during incubation. In addition, embryos dead or showing development retardation by day 25 of incubation contained copious LPS in allantoic fluid, in contrast to the negligible amount in normal developing embryos. Raising on water stocking density elevated bacteria counts, LPS concentrations in water and geese plasma, and decreased egg fertility but increased embryo mortality during incubation. In experiment 2, exogenous LPS treatment to the geese depressed egg laying, reduced egg hatchability, caused sickness behavior in the goslings hatched. In experiment 3, exogenous LPS directly administered to day 8 and 18 embryos during incubation dose dependently increased mortality and decreased hatchability, and caused sickness behavior in the goslings hatched. It is concluded that the raising on water geese stocking density stimulates pathogenic bacteria growth in water, which <em>via</em> LPS contamination impaires embryo development in incubation and therefore reduces geese reproductive performance and gosling quality during the hot summer months.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 9","pages":"Pages 1459-1466"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60139-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56761702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Defined siRNAs Suppress Nanog and Sox2 Expressions in Mouse ES Cells","authors":"Lei LEI,&nbsp;Lin DOU,&nbsp;Hua-yan WANG","doi":"10.1016/S1671-2927(11)60141-7","DOIUrl":"10.1016/S1671-2927(11)60141-7","url":null,"abstract":"<div><p><em>Nanog, Oct4</em> and <em>Sox2</em> are important transcription factors that are expressed in embryonic stem (ES) cells or embryonic carcinoma (EC) cells, but in most cases they are absent in somatic cells. These factors play a key role to maintain embryonic stem cell self-renew and pluripotency. Down-regulation of <em>Nanog</em> and <em>Sox2</em> gene expression can change multiple gene expression patterns and signal transduction pathways, and will initiate ES cell differentiation. This study was designed to select the efficient small interfering RNA (siRNA) fragments that inhibit <em>Nanog</em> and <em>Sox2</em> gene expression in mouse Jl ES cells and P19 EC cells. Among synthesized siRNAs we screened out the siRNAN301 for <em>Nanog</em> and siRNA S720 for <em>Sox2</em>, which not only down-regulated of <em>Nanog</em> and <em>Sox2</em> gene expression, but also interfered embryoid bodies formation. Our study provided the defined siRNA fragments that could be used to investigate the epigenetic function of <em>Nanog</em> and <em>Sox2</em> genes.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 9","pages":"Pages 1475-1481"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60141-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56761764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Eph-Ephrin A Molecules in Endometrium During Swine Embryo Implantation Examined Using Real-Time RT-PCR","authors":"Yan-feng FU,&nbsp;Jin-luan FU,&nbsp;Lu YANG,&nbsp;Ming-ming TIAN,&nbsp;Wen-cheng CHEN,&nbsp;Ai-guo WANG","doi":"10.1016/S1671-2927(11)60137-5","DOIUrl":"10.1016/S1671-2927(11)60137-5","url":null,"abstract":"<div><p>Erythropoietin-producing hepatocellular receptor and its membrane-bound ligands (Eph-Ephrin) system could regulate some mammalian blastocyst attachment and spreading. In order to investigate the involvement of the Eph-Ephrin system in swine embryo attachment, mRNA expression of Eph-Ephrin molecules in endometrium was examined by real-time RT-PCR during embryo implantation in pigs. The results indicated that mRNA expressions of Eph A5, A7 and Ephrin A5 all continually increased from pregnancy day 13 to 24. Ephrin A3 mRNA expression significantly increased from day 13 to 18 and decreased from day 18 to 24, and the expression was the lowest on pregnancy day 13 and the highest on day 18. However, Ephrin A4 mRNA expression was the lowest on pregnancy day 18 and the highest on day 24, and the expression decreased from day 13 to 18 and increased from day 18 to 24. Furthermore, mRNA expressions of Eph A5 and A7 were both found in other tissues, such as brain, muscle, intestine, stomach, etc. These findings suggest that the Eph-Ephrin system may play an important role in regulating the contact between blastocysts and endometrium during swine embryo implantation.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 9","pages":"Pages 1445-1451"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60137-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56761682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP, ISSR and RAPD Markers","authors":"Guang-qing ZHANG ,&nbsp;Jian-min QI ,&nbsp;Xiao-chen ZHANG ,&nbsp;Ping-ping FNAG ,&nbsp;Jian-guang SU ,&nbsp;Ai-fen TAO ,&nbsp;Tao LAN ,&nbsp;Wei-ren WU ,&nbsp;Ai-min LIU","doi":"10.1016/S1671-2927(11)60127-2","DOIUrl":"10.1016/S1671-2927(11)60127-2","url":null,"abstract":"<div><p>Kenaf (<em>Hibiscus cannabinus</em> L.) is one of the most economically important crops for non-wood fiber production. The objective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semi-wild variety Ga42 and a cultivar Alain kenaf were used as parents to construct an F₂ population consisting of 155 plants. The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplified polymorphism (SRAP), 56 inter-simple sequence repeat (ISSR), and 13 randomly amplified polymorphic DNA (RAPD) markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM between the adjacent markers.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 9","pages":"Pages 1346-1353"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60127-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56761994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Effect of Cinnamon Oil Combined with Thyme or Clove Oil","authors":"Fei LU ,&nbsp;Yi-cheng DING ,&nbsp;Xing-qian YE ,&nbsp;Yu-ting DING","doi":"10.1016/S1671-2927(11)60142-9","DOIUrl":"10.1016/S1671-2927(11)60142-9","url":null,"abstract":"<div><p>Antibacterial effect of cinnamon oil combined with thyme or clove oil was studied in this paper. Agar dilution method was used to determine the minimum inhibitory concentrations (MICs) of the essential oils (EOs) of cinnamon, thyme, and clove oil against three Gram-positive bacteria (<em>Bacillus subtilis, Bacillus cereus</em>, and <em>Staphylococcus aureus</em>), and two Gram-negative bacteria (<em>Escherichia coli</em> and <em>Salmonella typhimurium).</em> The results showed that cinnamon was a promising antibacterial substance with MIC ranged from 0.1 to 0.4 μL mL⁻¹ for the five bacterial species. Agar dilution checkerboard method was used to test the combined antibacterial effect of cinnamon oil with thyme or clove oil. Combination of cinnamon and thyme oil showed an additive effect against all selected bacteria, and combination of cinnamon and clove oil displayed an additive effect against <em>B. subtilis, B. cereus, S. aureus</em>, and an indifferent effect against <em>E. coli</em> and <em>S. typhimurium.</em> Furthermore, gas chromatography-mass spectrometry (GC-MS) measurement was used to analyze the components of the EOs, and the main components of cinnamon, thyme, and clove were cinnamaldehyde, thymol, carvacrol and/<em>p</em>-cymene, and eugenol, respectively.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 9","pages":"Pages 1482-1487"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60142-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56761783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Hina Gene Sequences in Wild (Hordeum spontaneum) and Cultivated (H. vulgare) Barleys","authors":"Wei-tao LI ,&nbsp;Qian-tao JIANG ,&nbsp;Guo-yue CHEN ,&nbsp;Zhi-en PU ,&nbsp;Ya-xi LIU ,&nbsp;Ji-rui WANG ,&nbsp;You-liang ZHENG ,&nbsp;Yu-ming WEI","doi":"10.1016/S1671-2927(11)60124-7","DOIUrl":"10.1016/S1671-2927(11)60124-7","url":null,"abstract":"<div><p>The <em>Hina</em> gene is one of the two known <em>Hin</em> genes for hardness, and its RNA expression is correlated with grain hardness and dry matter digestibility variation. In this study, only one clone of <em>Hina</em> gene was obtained from one barley accession. A total of 121 <em>Hina</em> gene sequences were isolated from 121 wild barley <em>{Hordeum spontaneum)</em> accessions in Israel, Iran, and Turkey, and then their molecular characteristics were compared with 97 <em>Hina</em> gene sequences from 74 cultivated barley <em>(H. vulgäre)</em> lines in Europe and 23 landrace <em>(H. vulgäre)</em> with global distribution and other 26 <em>Hina</em> gene sequences from cultivated barleys <em>(H. vulgäre)</em> with unknown global distribution. C/s-acting regulatory element (CARE) searching revealed that there were different types of regulatory element for the <em>Hina</em> gene in wild and landrace/cultivated barleys. There were six consistent cw-acting binding sites in wild and landrace/cultivated barleys, whereas 8 to 16 inconsistent TATA-boxes were observed. In addition, three special elements (E2Fb, Spl, and boxS) were only observed in wild barley, while one (AT 1 -motif) was only found in landrace/cultivated barley. Forty-four deduced amino acid sequences of HINA from wild and landrace/cultivated barleys were obtained by deleting repetitive amino acid sequences, and they were clustered into two groups on the basis of Neighbor-Joining analysis. However, there was no obvious difference in the amino acid sequences of HINA between wild and landrace/cultivated barleys. Comparing to protein secondary structure of wheat PINA, it was indicated that HINA also existed a signal peptide. In addition, HINA was a hydrophilic protein on the basis of the protein properties and composition.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 9","pages":"Pages 1313-1322"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60124-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56761885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in Organic C Mineralization Between Aerobic and Submerged Conditions in Paddy Soils of Southern Jiangsu Province, China","authors":"Rui-jun HAO ,&nbsp;Zhong-pei LI ,&nbsp;Yu-ping CHE","doi":"10.1016/S1671-2927(11)60134-X","DOIUrl":"10.1016/S1671-2927(11)60134-X","url":null,"abstract":"<div><p>Moisture regime plays a crucial role in the mineralization of soil organic carbon (SOC). In this paper, the dynamics of SOC mineralization in typical paddy soils of Changshu, Jiangsu Province, China, was investigated by incubation test in laboratory. The differences in SOC mineralization under aerobic and submerged conditions of paddy soils were also studied. Results showed that the daily mineralization of SOC under different moisture regimes was significantly different in the whole incubation period, at the beginning of the incubation, it decreased quickly under aerobic condition, but increased rapidly under submerged condition, and both remained constant after 10 d of incubation. The differences in SOC mineralization were found to be mainly at the beginning period of the incubation and decreased along with the incubation time. Thus, the difference was not significantly different at the later incubation period. The respiration intensity, daily and cumulative mineralization of SOC under aerobic condition was 2.26–19.11, 0.96–2.41, and 0.96–2.41 times than those under submerged condition, respectively. Statistic analyses showed that the higher the contents of microbial biomass carbon and nitrogen, the more significant difference in respiration intensity between aerobic and submerged conditions, but the higher the contents of microbial biomass nitrogen and dissolved organic carbon, the more significant difference in daily mineralization of SOC between the two conditions. The decrease in soil microbial activity under submerged condition was the main reason leading to the decrease in respiration intensity, but the decrease in SOC mineralization was also correlated with the changes in dissolved organic carbon over the whole incubation period.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 9","pages":"Pages 1410-1418"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60134-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A MicroRNA Catalog of Swine Umbilical Vein Endothelial Cells Identified by Deep Sequencing","authors":"Chen DAI,&nbsp;Yan-ming ZHANG,&nbsp;Qian ZHANG,&nbsp;Zong-song WU,&nbsp;Wen DENG,&nbsp;Xu ZHANG,&nbsp;Kang-kang GUO,&nbsp;Qing-hai TANG,&nbsp;Bo HOU","doi":"10.1016/S1671-2927(11)60140-5","DOIUrl":"10.1016/S1671-2927(11)60140-5","url":null,"abstract":"<div><p>MicroRNAs (miRNAs) are endogenous ∼22 nt RNAs that play important regulatory roles in targeting mRNAs for cleavage or translational repression. Despite the discovery of increasing numbers of human and mouse miRNAs, little is known about miRNAs from pig. In this study, we sought to extend the repertoire of porcine small regulatory RNAs using Solexa sequencing. We sequenced a library of small RNAs prepared from immortalized swine umbilical vein endothelial cells (SUVECs). We produced over 13.6 million short sequence reads, of which 8547658 perfectly mapped to the pig genome. A bioinformatics pipeline was used to identify authentic mature miRNA sequences. We identified 154 porcine miRNA genes, among which 146 were conserved across species, and 8 were pig-specific miRNA genes. The 146 miRNA genes encoded 116 conserved mature miRNAs and 66 miRNA*. The 8 pig-specific miRNA genes encoded 4 mature miRNAs. Four potential novel miRNAs were identified. The secondary structures of the 154 miRNA genes were predicted; 13 miRNAs have 2 structures, and miR-9 and miR-199 have 4 and 3 structures, respectively. 36 miRNAs were organized into 19 compact clusters. miR-206, miR-21 and miR-378 were the relatively highly expressed miRNAs. In conclusion, Solexa sequencing allowed the successful discovery of known and novel porcine miRNAs with high accuracy and efficiency. Furthermore, our results supply new data to the somewhat insufficient pig miRBase, and are useful for investigating features of the blood-brain barrier, vascular diseases and inflammation.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 9","pages":"Pages 1467-1474"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60140-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56761711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Transgenic Glyphosate-Resistant Rice with G6 Gene Encoding 5-Enolpyruvylshikimate-3-Phosphate Synthase","authors":"Te ZHAO,&nbsp;Chao-yang LIN,&nbsp;Zhi-cheng SHEN","doi":"10.1016/S1671-2927(11)60123-5","DOIUrl":"10.1016/S1671-2927(11)60123-5","url":null,"abstract":"<div><p>Glyphosate-resistant crops have been a huge economic success for genetic engineering. The creating of new glyphosate-resistant plants would increase the available choices for planting and lower the price of genetically modified crop seeds. A novel G6 gene from <em>Pseudomonas putida</em> that encoded 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was previously isolated. The G6 gene was transfected into rice <em>via Agrobacterium-mediated</em> transformation. The transgenic rice obtained was confirmed by PCR, Southern, and Western blots. The lab experiment and field trials further confirmed that the transgenic rice can survive glyphosate spraying at a dose of 8 g L⁻¹. In contrast, conventional rice was killed at a weed control glyphosate spray dose of 1 g L⁻¹. Altogether, the present study showed that the G6 gene works well in rice <em>in vivo</em> for glyphosate-resistance.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 9","pages":"Pages 1307-1312"},"PeriodicalIF":0.0,"publicationDate":"2011-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60123-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56761873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}